CN209024558U - A kind of cell culture bottle filter - Google Patents
A kind of cell culture bottle filter Download PDFInfo
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- CN209024558U CN209024558U CN201821705034.0U CN201821705034U CN209024558U CN 209024558 U CN209024558 U CN 209024558U CN 201821705034 U CN201821705034 U CN 201821705034U CN 209024558 U CN209024558 U CN 209024558U
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- filter
- cylinder ring
- cell culture
- cell
- culture bottle
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Abstract
The utility model discloses cell culture bottle filters, can effective filter out PBS and will not make cell conglomeration.A kind of cell culture bottle filter, including cylinder ring and filter mantle, filter mantle is mutually fixed with cylinder ring, and filter mantle stretches out and is exposed to the lower section of cylinder ring from the lower section of cylinder ring, the bottom of the cylinder ring is equipped with a circle groove, the slot opening is downward, and the lateral wall of groove is equipped with whorl of bottleneck, and the filter mantle encloses the closed bowl structure in bottom by multiple filters.
Description
Technical field
The utility model relates to field of cell culture, specially a kind of cell culture bottle filter.
Background technique
In attached cell culture, cell is covered with behind Tissue Culture Flask bottom to further cultivate, and is needed to pass on, be passed on
Add culture medium culture after needing to digest cell from Tissue Culture Flask with digestive ferment and then clean using PBS in the process.Mesh
Preceding common method is: being added after digestion enzymic digestion falls off to cell rounding after removing cell culture supernatant with containing 10% tire ox
PBS cleans cell after serum stops, and the cell after cleaning removes PBS after being centrifuged using 1000RPM 10min, but this process is extremely
It is few to need to clean twice, relatively time-consuming, it is unfavorable for improving efficiency, meanwhile, centrifugal process is only applicable to because of the limitation of method
Small-scale scientific research or research and development are difficult to realize large-scale industrialization production.In centrifugal process damage, shadow can be generated to cell
Ring the activity of cell next cultivated.And cell can generate some fragments during the growth process, and centrifugal process cannot be effective
Remove fragment.After centrifugation, cell tight is combined together under the action of centrifugal force and shearing force, can allow cell conglomeration, conglomeration
Cell be not easy to disperse or disperse not exclusively, then can make cell among cell mass in default of nutriment and it is dead,
Increase the death rate of cell.
Utility model content
The purpose of this utility model is to provide a kind of cell culture bottle filter, PBS and not can be effective filtered out
It can make cell conglomeration.
To achieve the above object, the utility model provides the following technical solutions:
A kind of cell culture bottle filter, including cylinder ring and filter mantle, filter mantle is mutually fixed with cylinder ring, and is filtered
The lower section for stretching out and being exposed to cylinder ring from the lower section of cylinder ring is covered, the bottom of the cylinder ring is equipped with a circle groove, described recessed
Channel opening is downward, and the lateral wall of groove is equipped with whorl of bottleneck, and the filter mantle encloses the closed bowl in bottom by multiple filters
Shape structure.
Preferred embodiment on the basis of above scheme and as above scheme: per a piece of filter (4) to mistake
The central concave of filter bowl.
Compared with prior art, the utility model has the beneficial effects that due on the cylinder ring of cell culture bottle filter
Equipped with whorl of bottleneck, filter device can be fixed on to the bottleneck of Tissue Culture Flask in application, operating process is very convenient, can be fast
Speed be filtered cleaning and etc., multiple filters be recessed inwardly enclose the closed bowl structure in bottom increase cell with
The contact area of filter effectively increases the efficiency of filtering, and the filter hole on filter can effectively remove cell
Fragment does not need to carry out other operations, the effect of the mechanical force avoided, greatly reduces the conglomeration rate of cell.This programme is set
The filter device of meter can manufacture different specifications according to production requirement, and the potential with extensive expanding production facilitates industry
Change production application.
Detailed description of the invention
Fig. 1 is the utility model section structural schematic diagram；
Fig. 2 is the utility model overall structure diagram.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
The utility model provides a kind of technical solution referring to FIG. 1-2: a kind of cell culture bottle filter, including cylinder
Ring 1, the cylinder ring 1 are in hollow structure, are equipped with a circle groove 2 in the bottom of cylinder ring 1, Open Side Down for the groove 2, groove
2 lateral wall is equipped with whorl of bottleneck.It whereby, in specific application, can be by cell culture bottle filter and Tissue Culture Flask phase
It screws up, the two is made to be fixed to each other.
The filter mantle 3 encloses the closed bowl structure in bottom by multiple filters 4, and the filter 4 is in blade
Shape and multiple filter hole are equipped on sheet body, aperture is routinely 0.75 μm, and the collar extension of the filter mantle 3 and 1 bottom of cylinder ring connect
It connects.
In the present embodiment, per a piece of filter 4 to the central concave of filter mantle.Since filter is tied at recess
It is whole to avoid the occurrence of filter hole caused by filter area deficiency so that there is filter biggish surface area to participate in filtering for structure
It is covered by cell fragment and loses filtering function.
Working principle: the filter device is equipped with the groove 2 with clamp device, and the lateral wall of groove 2 is equipped with whorl of bottleneck
Structure can cooperate with the bottleneck of Tissue Culture Flask tightens, so that filter device is fixed on the bottleneck of Tissue Culture Flask, operates
Journey is very convenient, can be rapidly performed by filtering cleaning and etc., multiple filters 4, which are recessed inwardly, encloses the closed bowl in bottom
Shape structure increases the contact area of cell Yu filter 4, effectively increases the efficiency of filtering, and 0.75 μ on filter 4
M filter hole can effectively remove cell fragment, not need to carry out other operations, and the effect of the mechanical force avoided is dropped significantly
The low conglomeration rate of cell.The filter device of this programme design can manufacture different specifications according to production requirement, have big rule
The potential of mould expanding production, facilitates industrialized production and application.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (2)
1. a kind of cell culture bottle filter, it is characterised in that: including cylinder ring (1) and filter mantle (3), filter mantle and cylinder ring
It is mutually fixed, and filter mantle stretches out and is exposed to the lower section of cylinder ring from the lower section of cylinder ring, and the bottom of the cylinder ring (1) is set
There is a circle groove (2), Open Side Down for the groove (2), and the lateral wall of groove is equipped with whorl of bottleneck, and the filter mantle (3) is by more
A filter (4) encloses the closed bowl structure in bottom.
2. cell culture bottle filter according to claim 1, it is characterised in that: per a piece of filter (4) to
The central concave of filter mantle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821705034.0U CN209024558U (en) | 2018-10-19 | 2018-10-19 | A kind of cell culture bottle filter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821705034.0U CN209024558U (en) | 2018-10-19 | 2018-10-19 | A kind of cell culture bottle filter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209024558U true CN209024558U (en) | 2019-06-25 |
Family
ID=66907948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821705034.0U Active CN209024558U (en) | 2018-10-19 | 2018-10-19 | A kind of cell culture bottle filter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209024558U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280733A (en) * | 2020-11-02 | 2021-01-29 | 上海坤爱生物科技股份有限公司 | Extraction method of amniotic stem cells |
-
2018
- 2018-10-19 CN CN201821705034.0U patent/CN209024558U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112280733A (en) * | 2020-11-02 | 2021-01-29 | 上海坤爱生物科技股份有限公司 | Extraction method of amniotic stem cells |
| CN112280733B (en) * | 2020-11-02 | 2024-02-23 | 上海坤爱生物科技股份有限公司 | Amniotic stem cell extraction method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 314000 room 14f1401-b, No. 705, Asia Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee after: Enlightenment Hemei Biotechnology (Jiaxing) Co.,Ltd. Address before: 314000 room 14f1401-b, No. 705, Asia Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee before: TUS-HEALCARE SCIENCE & TECHNOLOGY Co.,Ltd. Address after: 314000 room 14f1401-b, No. 705, Asia Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee after: Hemei Biotechnology (Zhejiang) Co.,Ltd. Address before: 314000 room 14f1401-b, No. 705, Asia Pacific Road, Nanhu District, Jiaxing City, Zhejiang Province Patentee before: Enlightenment Hemei Biotechnology (Jiaxing) Co.,Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhou Biliu Inventor after: Lu Yi Inventor after: Li Junxiang Inventor after: Dong Yunjia Inventor after: Yu Jianfeng Inventor after: Shen Lixia Inventor before: Zhou Biliu Inventor before: Lu Yi Inventor before: Li Junxiang Inventor before: Dong Yunjia Inventor before: Yu Jianfeng Inventor before: Shen Lixia |
|
| CB03 | Change of inventor or designer information |