CN208958107U - A kind of whole-field optically blood flow velocity analytical equipment - Google Patents
A kind of whole-field optically blood flow velocity analytical equipment Download PDFInfo
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- CN208958107U CN208958107U CN201721807382.4U CN201721807382U CN208958107U CN 208958107 U CN208958107 U CN 208958107U CN 201721807382 U CN201721807382 U CN 201721807382U CN 208958107 U CN208958107 U CN 208958107U
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Abstract
The utility model discloses a kind of whole-field optically blood flow velocity analytical equipments, including the vibration isolation optical platform being arranged in darkroom, and customised container, light source, fiber coupler, extension lens, diffusing glass, bracket, fixed frame, high-speed complimentary metal oxide semiconductor camera, zoom diplocardia decentered lens and computer on the vibration isolation optical platform are set, wherein, biological sample is placed in the customised container；The light source is connected by optical fiber with the fiber coupler；The utility model has studied the whole-field optically angiographic image of biopsy sample, and successfully highlights the red blood cell signal of background tissues；In conjunction with image processing techniques such as fuzzy partition, skeletal extractions blood vessel accurately can be partitioned into from whole-field optically angiographic image；The shortcomings that laser speckle can only measure blood flow relative velocity is overcome simultaneously, also overcomes the shortcomings that doppler Method can only could provide accurately lateral blood flow velocity measurement along incident light direction.
Description
Technical field
The utility model relates to blood flow velocity measurement technical fields, and in particular to a kind of analysis of whole-field optically blood flow velocity is set
It is standby.
Background technique
Currently, some optical image technologies have been applied to blood flow velocity measurement field, such as laser speckle imaging systems
With laser-Doppler imaging system: laser speckle imaging systems are mainly made of coherent source and image processing equipment, the technology
Blood flow velocity, coherent light random superposition after biological tissue scatters, figure are mainly obtained using the rear orientation light of biological tissue
As processing equipment random speckle pattern and is analyzed by acquisition, blood flow velocity is finally estimated；Laser-Doppler imaging system
System is based on Doppler effect, and measurement blood flow velocity is excited light beam and flows the shadow of the doppler angle of sample point flow direction
It rings, when doppler angle is 90 °, lateral blood flow velocity will be 0, and when doppler angle is 0 °, lateral blood flow velocity
Equal to tested speed.
But when carrying out blood flow velocity measurement using laser speckle imaging systems, since by environmental condition, (such as light source is strong
Degree, irradiating angle, imaging object and camera parameter) and biological tissue in scattering process (such as VELOCITY DISTRIBUTION, static speckles and more
Weight speckle) influence so that the blood flow velocity that laser speckle imaging method measures is relative velocity rather than absolute velocity, and deposit
In large error, therefore, this method can not accurately measure the lateral velocity of blood flow；In addition, by Doppler effect it is found that only
When the incident beam of laser is parallel to blood flow direction, blood flow velocity could be accurately obtained, therefore laser-Doppler imaging system
Accurately radial blood flow velocity measurement can only be provided along incident light direction, for some flat biological samples, such as chicken embryo, zebra
Fish and retina, imaging plane are perpendicular to incident light direction, and the direction of motion of blood flow be it is lateral, therefore, depend on
The laser-Doppler imaging system of Doppler effect is unable to measure out the lateral velocity of blood flow.
Utility model content
The shortcomings that the purpose of the utility model is to overcome the prior arts and deficiency, provide a kind of whole-field optically blood flow velocity
Analytical equipment, the equipment are mainly used for the blood of real-time measurement transparent organism sample (such as zebra fish, egg embryo) blood vessel middle line
Lateral velocity is flowed, the capillary network mode of pathological tissue is studied, studies the metabolism and embryonic development of cell.
The purpose of this utility model is achieved through the following technical solutions:
A kind of whole-field optically blood flow velocity analytical equipment, including the vibration isolation optical platform being arranged in darkroom, and setting
Customised container, light source, fiber coupler on the vibration isolation optical platform, extend lens, diffusing glass, bracket, fixed frame,
High-speed complimentary metal oxide semiconductor camera, zoom diplocardia decentered lens and computer, wherein placed in the customised container
Biological sample；The light source is connected by optical fiber with the fiber coupler；The extension lens setting is held in the customization
The camera lens for extending lens is arranged in the surrounding of device, and customised container described in the extension lens lighting, the diffusing glass
Before；The fiber coupler is connected by optical fiber with the extension lens；The bracket supports extension lens and described
Diffusing glass；
The high-speed complimentary metal oxide semiconductor camera is fixed on the vibration isolation optical platform by the fixed frame
On, and the high-speed complimentary metal oxide semiconductor camera is located in the surface of the customised container；The zoom diplocardia
Before the camera lens of the high-speed complimentary metal oxide semiconductor camera is arranged in decentered lens, and the zoom diplocardia decentered lens
Towards the customised container；
The light source and the high-speed complimentary metal oxide semiconductor camera are connected with the computer respectively.
Preferably, the steady temperature of the customised container is 37.8 DEG C, humidity 75%.
Preferably, the light source is fiber coupling light emitting diode.
Preferably, there are four the extension lens are set, it is separately positioned on the surrounding of the customised container, and described four are prolonged
It stretches lens and irradiates the customised container from four direction, the diffusing glass is also correspondingly provided with four, is separately positioned on described four
Before a camera lens for extending lens；The fiber coupler separates four optical fiber, is connected respectively with four extension lens；Institute
It states branch and is provided with eight, support four extension lens and four diffusing glasses respectively.
Working principle of the utility model is:
When work, adjusted first positioned at four extension lens of customised container surrounding and the height and photograph of diffusing glass
Firing angle degree, while adjusting the height of high-speed complimentary metal oxide semiconductor camera and zoom diplocardia decentered lens；It counts later
Calculation machine controls fiber coupling light emitting diode and emits light beam, which is divided into four collimated light beams after fiber coupler, should
Four collimated light beams are irradiated on laboratory sample after four extension lens and diffusing glass, and are reflected and overflow from laboratory sample
Light is penetrated, laboratory sample is located in the customised container that steady temperature is 37.8 DEG C and humidity is 75% at this time, and diffused light is from experiment sample
It is reflected on product and passes through zoom diplocardia decentered lens and injected in high-speed complimentary metal oxide semiconductor camera, computer control
High-speed complimentary metal oxide semiconductor camera shoots multiple continuous images and by these image transmitting to computer, is used for blood
The measurement of stream visualization and blood flow lateral velocity；
Multiple received consecutive images of computer disposal, first by the motor message (I of red blood cell (RBCs)AC) and background group
Motor message (the I knittedDC) separate, since two kinds of substances have differences the absorbability of light, between the two to produce not
Same frequency signal, therefore make red blood cell (RBCs) motor message (IAC) and background tissues motor message (IDC) divide in a frequency domain
From original time signal is transferred to frequency (f) domain from time (t) domain by Fast Fourier Transform, then passes through height respectively
Pass filter and low-pass filtering obtain the absorbability of red blood cell (RBCs) and background tissues, as follows using formula:
Wherein HPF () and LPF () respectively indicate high-pass filtering and low-pass filtering；I (x, y) is collected original image,
X, y respectively indicate the abscissa and ordinate of pixel in I, and I (x, y, t) is collected sequence image, and I (x, y, f) is it
Frequency-region signal；In order to along vessel centerline measurement blood flow horizontal line speed (BFTV), and highlight the fortune of red blood cell (RBCs)
Dynamic signal, can be according to IAC(x, y, t) and IDCThe ratio of (x, y, t) obtains the sequence image of instantaneous modulation depth (IMD):
And the whole-field optically angiogram of average modulation depth (AMD) relevant to red blood cell (RBCs) movement can be under
Formula obtains:
WhereinWithIt is the average absolute of Dynamic Signal and stationary singnal over time respectively
The whole-field optically blood vessel that value, vessel centerline and blood flow lateral velocity (BFTV) may map to average modulation depth (AMD) is made
On shadow image；
It, can be from wink after using equation (1) separating red corpuscle (RBCs) motor message and background tissues motor message
When modulation depth (IMD) sequence image in directly acquire multidate information about blood microcirculation, select position A to be measured, count
The cross-correlation coefficient of A and neighbouring pixel B are calculated, maximum two points of cross-correlation coefficient are selected, further according between the two points
Blood flow lateral velocity (BFTV) can be obtained in displacement and transition time, and calculation formula is as follows:
Wherein RAB(τ) is instantaneous modulation depth (IMD) cross-correlation coefficient between A and B, and τ is between two relevant positions
Conversion time, be equal to RABDelay time between the top and zero moment of (τ)；
Wherein, since blood-vessel image is whole-field optically angiographic image, superposition phenomenon can occur for blood vessel, cause to be permitted
Mostly different blood vessels is erroneously interpreted as identical blood vessel, this will cause difficulty in image procossing and analysis, in some instances it may even be possible to cause
Therefore the experimental result of mistake for the ease of subsequent analysis and observation, needs to distinguish different blood vessels, therefore uses absolute mould
: there is fuzzy syntople in paste connection partitioning algorithm, be divided into On Local Fuzzy relationship and complete between any two pixel in image
Office's fuzzy relation；On Local Fuzzy relationship between pixel, also referred to as fuzzy affinity, value is within the scope of 0-1, it and two phases
The spatial proximity of adjacent pixel (c, d), even intensity degree are related with intensity similarity；Global fuzzy relation between pixel,
Also referred to as fuzzy connectedness, value is also within the scope of 0-1, and for determining global fuzzy relation, this is related to really On Local Fuzzy relationship
Determine the On Local Fuzzy relation value of two adjacent pixels in each path between c and d, and is used as path using minimum one
Intensity, then determine the highest global fuzzy relation as between pixel c and d in all path intensity values；Whole-field optically
The fuzzy adjoining of angiographic image is defined as:
Wherein k1Nonnegative constant, c, d are two adjacent pixels in whole-field optically angiographic image, n be c and
Number of path between d.
Affinity in whole-field optically angiographic image between any two pixel (c, d) is defined as:
Wherein μω(c, d) is the fuzzy adjoining that equation defines, k2It is nonnegative constant；
The global fuzzy relation of whole-field optically angiographic image is given by:
Wherein i is integer and 1≤i≤n, n are the total number of paths from c to d；
Wherein c0,c1,...,ci,ci+1,...,cm+1It is one group of pixel sequence；ciAnd ci+1It is the two of its four adjacent pixels
A adjacent pixel；c0=c；cm+1=d；When μ (c, d) is greater than suitable threshold value, whole-field optically angiographic image can be obtained
In blood-vessel image with background separation, simultaneously as the value of μ (c, d) is between 0-1, therefore the threshold value selected must be in this model
In enclosing；
Wherein, when using absolute fuzzy connectedness segmentation algorithm, needing will be all in whole-field optically angiographic image
Blood vessel is separated from image background, to obtain the bianry image of blood vessel, uses identical absolute fuzzy connectedness segmentation algorithm
Separate the blood vessel that overlaps each other in whole-field optically angiographic image, after obtaining the image, further refinement blood vessel with
The center line for determining them makes image skeletonization using topological method, in the deletable pixel of the boundary definition of image object, with
The skeleton for obtaining image object needs to keep the connectivity of image object while definition can delete pixel, and does not change
The hole count in number of objects, target in image and the positional relationship between different objects；In all directions due to object
Uniform shrinkage, and obtained skeleton line is placed on the center of the object, so the skeleton line of the object is exactly its center line.
The utility model have compared with prior art it is below the utility model has the advantages that
(1) the utility model has studied the whole-field optically angiographic image of biopsy sample, and successfully highlights background group
The red blood cell signal knitted；It can accurately be made from whole-field optically blood vessel in conjunction with image processing techniques such as fuzzy partition, skeletal extractions
Blood vessel is partitioned into shadow image；The shortcomings that laser speckle can only measure blood flow relative velocity is overcome simultaneously, how general is also overcomed
Le method can only could provide the shortcomings that accurately lateral blood flow velocity measurement along incident light direction；
(2) the utility model can distinguish the blood vessel being overlapped in whole-field optically angiographic image, and be calculated using cross-correlation
Method has carried out accurate measurement to the absolute lateral blood flow velocity of vessel centerline, can be used for studying the capillary of pathological tissue
Network mode can be used for the metabolism and embryonic development of research cell.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model whole-field optically blood flow velocity analytical equipment；
Fig. 2 is the schematic diagram that the utility model absorption intensity fluctuates mudulation effect (AIFM)；
Fig. 3 is the schematic diagram that the utility model red blood cell (RBCs) signal is separated with background signal；
Fig. 4 is the flow chart of the utility model whole-field optically blood flow velocity analysis method.
Appended drawing reference in figure are as follows: 1, vibration isolation optical platform；2, customised container；3, light source；4, fiber coupler；5, extend saturating
Mirror；6, diffusing glass；7, bracket；8, fixed frame；9, high-speed complimentary metal oxide semiconductor camera；10, zoom diplocardia is eccentric
Lens；11, computer.
Specific embodiment
The present invention will be further described in detail with reference to the embodiments and the accompanying drawings, but the implementation of the utility model
Mode is without being limited thereto.
As shown in figures 1-4, a kind of whole-field optically blood flow velocity analytical equipment, including the vibration isolation optics being arranged in darkroom
Platform 1, and be arranged on the vibration isolation optical platform 1 customised container 2, light source 3, fiber coupler 4, extend lens 5, be unrestrained
It penetrates glass 6, bracket 7, fixed frame 8, high-speed complimentary metal oxide semiconductor camera 9, zoom diplocardia decentered lens 10 and calculates
Machine 11, wherein biological sample is placed in the customised container 2, the steady temperature of the customised container 2 is 37.8 DEG C, and humidity is
75%；The light source 3 is connected by optical fiber with the fiber coupler 4, and the light source 3 is fiber coupling light emitting diode；
The surrounding for extending lens 5 and the customised container 2 being set, and the extension lens 5 irradiate the customised container 2, it is described
Before the camera lens for extending lens 5 is arranged in diffusing glass 6；The fiber coupler 4 passes through optical fiber and 5 phase of extension lens
Connection；The bracket 7 supports the extension lens 5 and the diffusing glass 6, and specifically, the extension lens 5 are equipped with four
It is a, it is separately positioned on the surrounding of the customised container 2, and four extension lens 5 irradiate the customization from four direction and hold
Device 2, the diffusing glass 6 are also correspondingly provided with four, before being separately positioned on the camera lens of four extension lens 5；The optical fiber
Coupler 4 separates four optical fiber, is connected respectively with four extension lens 5；The bracket 7 is equipped with eight, supports respectively
Four extension lens 5 and four diffusing glasses 6；The high-speed complimentary metal oxide semiconductor camera 9 passes through institute
It states fixed frame 8 to be fixed on the vibration isolation optical platform 1, and the high-speed complimentary metal oxide semiconductor camera 9 is located at
The surface of the customised container 2；The setting of zoom diplocardia decentered lens 10 is partly led in the high-speed complimentary metal oxide
Before the camera lens of body camera 9, and the zoom diplocardia decentered lens 10 is towards the customised container 2；The light source 3 and the high speed
CMOS camera 9 is connected with the computer 11 respectively.
Due to the direction of motion of blood flow be it is lateral, need a kind of tracking red blood cell (RBCs) motion profile and blood flow horizontal
To the new method of tachometric survey, therefore the utility model proposes a kind of whole-field optically blood flow velocity analysis methods, due to red thin
There are absorption differences under the low-coherent light irradiation of 540nm central wavelength for born of the same parents (RBCs) and background tissues, as red blood cell (RBCs)
When discontinuously passing through blood vessel, causes the fluctuation of endogenous instantaneous strength, generate the fluctuation signal of high frequency, it is strong to be then formed absorption
Degree fluctuation mudulation effect, fluctuates mudulation effect according to absorption intensity, deep by calculating average modulation depth (AMD) and instantaneous modulation
It spends (IMD), can get whole-field optically angiographic image and red blood cell (RBCs) signal relative to background tissues, it then can be with
Vessel centerline is determined from average modulation depth (AMD) image, finally according to instantaneous modulation depth (IMD) sequence image and formula
ν=Δ L/ Δ τ AB is calculated along the delay time (Δ τ AB) and phase between two mutual off position A and B of vessel centerline
To displacement (Δ L), the blood flow horizontal line speed (BFTV) of vessel centerline can be calculated.And under low-coherent light irradiation, it is red thin
Born of the same parents' (RBCs) absorption coefficient of light is higher than background tissues, as shown in Fig. 2, drawing when red blood cell (RBCs) discontinuously passes through blood vessel
The fluctuation of endogenous instantaneous strength is sent out, high-frequency signal, but background group are become by the time reflex luminous intensity of camera pixel record at this time
It knits the instantaneous strength fluctuation due to no any interior life and only generates direct current signal, this physical mechanism is exactly absorption intensity fluctuation
(AIFM) effect is modulated, then this phenomenon be used to realize accurate blood flow horizontal line speed (BFTV) measurement.
The working principle of the utility model are as follows: collect the original image of multiple m × w pixels by camera first, then
Fast Fourier Transform (FFT) is done on a timeline to m × w pixel, stationary singnal separation of taking action of going forward side by side obtains the fortune of red blood cell
Dynamic signal and background signal obtain average modulation depth figure using the motor message of red blood cell and background signal later and instantaneously adjust
Depth sequence chart processed isolates blood-vessel image from average modulation depth figure followed by fuzzy partition algorithm, according to blood vessel
Bianry image obtains the Graph of blood vessel using topological method, further according to average modulation depth figure, the bianry image of blood vessel
And the Graph synthesis of blood vessel only includes the blood-vessel image of blood vessel and blood vessel middle line, finally according to the composite diagram of blood vessel and instantaneously
Modulation depth sequence chart carries out cross-correlation analysis to the same red blood cell simultaneously through upper two o'clock of crossing the centre line, can be obtained blood flow
Lateral velocity.
Specifically, when work, the four extension lens 5 and diffusing glass 6 positioned at 2 surrounding of customised container are adjusted first
Height and irradiating angle, while adjusting high-speed complimentary metal oxide semiconductor camera 9 and zoom diplocardia decentered lens
10 height；Computer 11 controls fiber coupling light emitting diode transmitting light beam later, which divides after fiber coupler 4
At four collimated light beams, which is irradiated on laboratory sample after four extension lens 5 and diffusing glass 6,
And the reflected diffusion light from laboratory sample, the customization that laboratory sample is located at that steady temperature is 37.8 DEG C and humidity is 75% at this time are held
In device 2, diffused light, which reflects from laboratory sample and passes through zoom diplocardia decentered lens 10, injects high-speed complimentary metal oxide
In semiconductor camera 9, computer 11 controls multiple the continuous images of shooting of high-speed complimentary metal oxide semiconductor camera 9 and will
Measurement of these image transmittings to computer 11, for blood flow visualization and blood flow lateral velocity；
As shown in figs. 34, computer 11 handles multiple received consecutive images, first by the movement of red blood cell (RBCs)
Signal (IAC) with the motor message (I of background tissuesDC) separate, since two kinds of substances have differences the absorbability of light, the two
Between to producing different frequency signals, therefore make red blood cell (RBCs) motor message (IAC) and background tissues motor message
(IDC) separate in a frequency domain, original time signal is transferred to from time (t) domain by frequency (f) domain by Fast Fourier Transform,
Then the absorbability of red blood cell (RBCs) and background tissues are obtained by high-pass filtering and low-pass filtering respectively, such as using formula
Under:
Wherein HPF () and LPF () respectively indicate high-pass filtering and low-pass filtering；I (x, y) is collected original image,
X, y respectively indicate the abscissa and ordinate of pixel in I, and I (x, y, t) is collected sequence image, and I (x, y, f) is it
Frequency-region signal；In order to along vessel centerline measurement blood flow horizontal line speed (BFTV), and highlight the fortune of red blood cell (RBCs)
Dynamic signal, can be according to IAC(x, y, t) and IDCThe ratio of (x, y, t) come obtain instantaneous modulation depth (IMD,
Instantaneous modulation depth) sequence image:
And average modulation depth (AMD, average modulation depth) relevant to red blood cell (RBCs) movement
Whole-field optically angiogram can be obtained by following formula:
WhereinWithIt is the average absolute of Dynamic Signal and stationary singnal over time respectively
The whole-field optically blood vessel that value, vessel centerline and blood flow lateral velocity (BFTV) may map to average modulation depth (AMD) is made
On shadow image；
It, can be from wink after using equation (1) separating red corpuscle (RBCs) motor message and background tissues motor message
When modulation depth (IMD) sequence image in directly acquire multidate information about blood microcirculation, select position A to be measured, count
The cross-correlation coefficient of A and neighbouring pixel B are calculated, maximum two points of cross-correlation coefficient are selected, further according between the two points
Blood flow lateral velocity (BFTV) can be obtained in displacement and transition time, and calculation formula is as follows:
Wherein RAB(τ) is instantaneous modulation depth (IMD) cross-correlation coefficient between A and B, and τ is between two relevant positions
Conversion time, be equal to RABDelay time between the top and zero moment of (τ)；
Wherein, since blood-vessel image is whole-field optically angiographic image, superposition phenomenon can occur for blood vessel, cause to be permitted
Mostly different blood vessels is erroneously interpreted as identical blood vessel, this will cause difficulty in image procossing and analysis, in some instances it may even be possible to cause
Therefore the experimental result of mistake for the ease of subsequent analysis and observation, needs to distinguish different blood vessels, therefore uses absolute mould
: there is fuzzy syntople in paste connection partitioning algorithm, be divided into On Local Fuzzy relationship and complete between any two pixel in image
Office's fuzzy relation；On Local Fuzzy relationship between pixel, also referred to as fuzzy affinity, value is within the scope of 0-1, it and two phases
The spatial proximity of adjacent pixel (c, d), even intensity degree are related with intensity similarity；Global fuzzy relation between pixel,
Also referred to as fuzzy connectedness, value is also within the scope of 0-1, and for determining global fuzzy relation, this is related to really On Local Fuzzy relationship
Determine the On Local Fuzzy relation value of two adjacent pixels in each path between c and d, and is used as path using minimum one
Intensity, then determine the highest global fuzzy relation as between pixel c and d in all path intensity values；Whole-field optically
The fuzzy adjoining of angiographic image is defined as:
Wherein k1Nonnegative constant, c, d are two adjacent pixels in whole-field optically angiographic image, n be c and
Number of path between d.
Affinity in whole-field optically angiographic image between any two pixel (c, d) is defined as:
Wherein μω(c, d) is the fuzzy adjoining that equation defines, k2It is nonnegative constant；
The global fuzzy relation of whole-field optically angiographic image is given by:
Wherein i is integer and 1≤i≤n, n are the total number of paths from c to d；
Wherein c0,c1,...,ci,ci+1,...,cm+1It is one group of pixel sequence；ciAnd ci+1It is the two of its four adjacent pixels
A adjacent pixel；c0=c；cm+1=d；When μ (c, d) is greater than suitable threshold value, whole-field optically angiographic image can be obtained
In blood-vessel image with background separation, simultaneously as the value of μ (c, d) is between 0-1, therefore the threshold value selected must be in this model
In enclosing；
Wherein, when using absolute fuzzy connectedness segmentation algorithm, needing will be all in whole-field optically angiographic image
Blood vessel is separated from image background, to obtain the bianry image of blood vessel, uses identical absolute fuzzy connectedness segmentation algorithm
Separate the blood vessel that overlaps each other in whole-field optically angiographic image, after obtaining the image, further refinement blood vessel with
The center line for determining them makes image skeletonization using topological method, in the deletable pixel of the boundary definition of image object, with
The skeleton for obtaining image object needs to keep the connectivity of image object while definition can delete pixel, and does not change
The hole count in number of objects, target in image and the positional relationship between different objects；In all directions due to object
Uniform shrinkage, and obtained skeleton line is placed on the center of the object, so the skeleton line of the object is exactly its center line.
The utility model has studied the whole-field optically angiographic image of biopsy sample, and successfully highlights background tissues
Red blood cell signal；It can be accurately from whole-field optically angiogram in conjunction with image processing techniques such as fuzzy partition, skeletal extractions
Blood vessel is partitioned into as in；The shortcomings that laser speckle can only measure blood flow relative velocity is overcome simultaneously, also overcomes Doppler side
Method can only could provide the shortcomings that accurately lateral blood flow velocity measurement along incident light direction；The utility model can distinguish the whole audience
The blood vessel being overlapped in optical vessel contrastographic picture, and using cross correlation algorithm to the absolute lateral blood flow velocity of vessel centerline into
It has gone accurate measurement, can be used for studying the capillary network mode of pathological tissue, can be used for the new old of research cell
Metabolism and embryonic development.
Above-mentioned is the preferable embodiment of the utility model, but the embodiments of the present invention is not by above content
It limits, it is made under other any spiritual essence and principles without departing from the utility model to change, modification, substitution, combine, letter
Change, should be equivalent substitute mode, be included within the protection scope of the utility model.
Claims (4)
1. a kind of whole-field optically blood flow velocity analytical equipment, which is characterized in that including the vibration isolation optical platform being arranged in darkroom,
And customised container on the vibration isolation optical platform is set, light source, fiber coupler, extends lens, diffusing glass, branch
Frame, fixed frame, high-speed complimentary metal oxide semiconductor camera, zoom diplocardia decentered lens and computer, wherein the customization
Biological sample is placed in container；The light source is connected by optical fiber with the fiber coupler；The extension lens setting exists
The surrounding of the customised container, and customised container described in the extension lens lighting, the diffusing glass are arranged in the extension
Before the camera lens of lens；The fiber coupler is connected by optical fiber with the extension lens；The bracket supports the extension
Lens and the diffusing glass；
The high-speed complimentary metal oxide semiconductor camera is fixed on the vibration isolation optical platform by the fixed frame, and
The high-speed complimentary metal oxide semiconductor camera is located in the surface of the customised container；The zoom diplocardia is eccentric thoroughly
Before the camera lens of the high-speed complimentary metal oxide semiconductor camera is arranged in mirror, and the zoom diplocardia decentered lens is towards institute
State customised container；
The light source and the high-speed complimentary metal oxide semiconductor camera are connected with the computer respectively.
2. whole-field optically blood flow velocity analytical equipment according to claim 1, which is characterized in that the perseverance of the customised container
Determining temperature is 37.8 DEG C, humidity 75%.
3. whole-field optically blood flow velocity analytical equipment according to claim 1, which is characterized in that the light source is optical fiber coupling
Close light emitting diode.
4. whole-field optically blood flow velocity analytical equipment according to claim 1, which is characterized in that the extension lens are equipped with
Four, it is separately positioned on the surrounding of the customised container, and four extension lens irradiate the customization from four direction and hold
Device, the diffusing glass are also correspondingly provided with four, before being separately positioned on the camera lens of four extension lens；The fiber coupling
Device separates four optical fiber, is connected respectively with four extension lens；The branch is provided with eight, supports described four respectively
Extend lens and four diffusing glasses.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721807382.4U CN208958107U (en) | 2017-12-21 | 2017-12-21 | A kind of whole-field optically blood flow velocity analytical equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721807382.4U CN208958107U (en) | 2017-12-21 | 2017-12-21 | A kind of whole-field optically blood flow velocity analytical equipment |
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| Publication Number | Publication Date |
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| CN208958107U true CN208958107U (en) | 2019-06-11 |
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| CN201721807382.4U Expired - Fee Related CN208958107U (en) | 2017-12-21 | 2017-12-21 | A kind of whole-field optically blood flow velocity analytical equipment |
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- 2017-12-21 CN CN201721807382.4U patent/CN208958107U/en not_active Expired - Fee Related
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