CN208887965U - A kind of gel device for cutting - Google Patents
A kind of gel device for cutting Download PDFInfo
- Publication number
- CN208887965U CN208887965U CN201821434291.5U CN201821434291U CN208887965U CN 208887965 U CN208887965 U CN 208887965U CN 201821434291 U CN201821434291 U CN 201821434291U CN 208887965 U CN208887965 U CN 208887965U
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- CN
- China
- Prior art keywords
- cutting
- crossbeam
- lag
- offset plate
- sliding slot
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Abstract
The utility model discloses a kind of gel device for cutting, including U-lag, plastic cutting frame, offset plate and gage are cut, plastic cutting frame is across the two sides of U-lag, and it is slidably connected with the two sides of U-lag, it cuts offset plate to be vertically arranged in plastic cutting frame, and is slidably connected with plastic cutting frame, the inside of U-lag is arranged in gage.The utility model discloses a kind of gel device for cutting, according to target protein molecular size range, transferring film is carried out again after accurately cutting the PAGE gel of the band containing target protein, not only avoids waste PVDF/NC film, it can also avoid accidentally cutting target protein band, to improve the success rate of experiment.
Description
Technical field
The utility model relates to bioengineering equipment technical field, more particularly to a kind of gel device for cutting.
Background technique
In Western Blot experimentation, after completing protein electrophoresis separation, needing will be in PAGE gel
Protein band goes on pvdf membrane or NC film and carries out subsequent detection.Since protein electrophoresis can be realized together according to molecular weight of albumen size
It is detected while one sample difference albumen, PVDF/NC film and antibody price are higher in addition, so researcher often will be containing only target
The PVDF/NC film of albumen carries out antibody incubation, colour developing and band detection.
Wherein, antibody incubation is carried out to the PVDF/NC film containing target protein, the common method that colour developing and band detect has two
Kind: first, according to Marker bar band, then cutting band containing target protein after whole PAGE gel is carried out transferring film
PVDF/NC film detected, but this method can cause certain waste to PVDF/NC film, and carry out half-dried turn with this method
When film detects multiple protein, due to the difference between molecular weight of albumen, large molecular weight protein transferring film is not thorough, small molecular weight protein
Pvdf membrane is produced, sample is caused to waste；Second, cutting the SDS- of the band containing target protein according to target protein molecular size range
Transferring film is carried out after PAGE gel again, but this method is easy accidentally to cut target protein band when cutting glue, leads to the failure of an experiment.
Therefore, how to provide a kind of gel device for cutting for accurately cutting the band containing target protein be those skilled in the art urgently
Problem to be solved.
Utility model content
In view of this, the present invention provides a kind of gel device for cutting, according to target protein molecular size range, accurately
Transferring film is carried out again after cutting the PAGE gel of the band containing target protein, is not only avoided waste PVDF/NC, can also be avoided accidentally cutting
Target protein band, to improve the success rate of experiment.
To achieve the goals above, the utility model adopts the following technical solution:
A kind of gel device for cutting, including U-lag, plastic cutting frame, offset plate and gage are cut, it is placed by the U-lag to be cut
The gel cut, the plastic cutting frame are slidably connected across the two sides of the U-lag, and with the two sides of the U-lag, so as to
The position of the plastic cutting frame is adjusted flexibly, the plastic cutting frame is conveniently made to bring the offset plate of cutting into position that gel is cut, it is described
It cuts offset plate to be vertically arranged in the plastic cutting frame, and is slidably connected with the plastic cutting frame, so as to make described to cut offset plate along institute
It states plastic cutting frame to fall vertically, offset plate is cut described in guarantee and vertically cuts gel, avoid during cutting gel, it is described to cut offset plate
Run-off the straight and lead to the problem of accidentally cutting target protein band, the inside of the U-lag is arranged in the gage, thus not only can be with
By comparing the scale on the gage, the location of Marker on gel is accurately positioned, and described in can accurately determining
The position that offset plate is cut is cut, keeps the location of the position for cutting offset plate and Marker on gel accurate corresponding, thus real
The personnel of testing can accurately cut the PAGE gel of the band containing target protein along Marker.
Preferably, the groove wall top of the U-lag two sides is provided with sliding slot, and the sliding slot two sides are respectively formed sliding slot
Outer wall and sliding slot inner wall.
Preferably, the gage is arranged on the sliding slot inner wall, so as to by being arranged on the sliding slot inner wall
The gage the location of gel Marker is accurately positioned, and record the scale value of the present position Marker, while institute
The scale value for cutting the offset plate alignment present position Marker is stated, so that described cut after offset plate is directed at the position Marker be made to go cutting solidifying again
Glue, to greatly improve the accuracy for cutting the PAGE gel of the band containing target protein.
Preferably, the limit sliding chutes parallel with the sliding slot are provided on the sliding slot outer wall.
Preferably, the plastic cutting frame is made of slide plate and crossbeam, the both ends of the crossbeam top with the slide plate respectively
Integrally connected, to enhance the firmness of the plastic cutting frame, and the bottom end of the slide plate is slidably connected with the sliding slot, to make
The relatively described U-lag of the plastic cutting frame is slidably connected, and the position of the plastic cutting frame is adjusted flexibly, conveniently makes the plastic cutting frame by institute
It states and cuts the position that offset plate brings gel cutting into.
Preferably, the bottom end of the slide plate is vertically and fixedly provided with traveller, and the traveller is slidably connected with the limit sliding chutes, makes
The relatively described U-lag of the plastic cutting frame is slidably connected, and the position of the plastic cutting frame is adjusted flexibly, conveniently makes the plastic cutting frame by institute
It states and cuts the position that offset plate brings gel cutting into, and the traveller passes through the limit sliding chutes threaded coupling nut, thus when looking for
When the position of quasi- cutting gel, the nut and the traveller can be fastened, so that the traveller is temporarily fixed to the limit and slides
Between the cell wall of slot and the nut, to avoid plastic cutting frame when cutting gel that dislocation occurs or shakes and causes accidentally to cut mesh
The problem of marking protein band.
Preferably, horizontally disposed crossbeam cuts glue groove on the crossbeam, and the offset plate of cutting is vertically arranged in the cross
Beam is cut in glue groove, and is cut glue groove with the crossbeam and be slidably connected, so that the utility model can make described to cut offset plate along the cross
Beam is cut glue groove and is fallen vertically, prevent it is described cut offset plate and move horizontally, ensure that the offset plate of cutting vertically cuts gel, avoid
It is described to cut offset plate run-off the straight and lead to the problem of accidentally cutting target protein band during cutting gel.
Preferably, the crossbeam and the both ends for cutting offset plate are provided with location hole, and location hole matching connects
Positioning pin is connect, so as to realize described any one position cutting offset plate and being located in the plastic cutting frame and cut described in protection
The effect of offset plate.
Preferably, it is provided with handle guide rail along the vertical direction on the crossbeam.
Preferably, the both ends for cutting offset plate are formed with manipulation handle, and the manipulation handle and the handle guide rail are sliding
Dynamic connection makes to operate so as to facilitate the height for cutting offset plate relative gel described in control by controlling the manipulation handle
Process is simple.
It can be seen via above technical scheme that compared with prior art, the utility model, which discloses, to be provided a kind of gel and cuts
Device is taken, following technical effect may be implemented:
1. plastic cutting frame is slidably connected across the two sides of the U-lag, and with the two sides of U-lag, so as to be adjusted flexibly
The position of plastic cutting frame conveniently makes plastic cutting frame that will cut offset plate and brings the position that gel is cut into, simple to operate；
2. cutting the crossbeam that offset plate is vertically arranged in plastic cutting frame to cut in glue groove, moved horizontally to prevent from cutting offset plate,
Make to cut offset plate and cut glue groove along crossbeam and fall vertically, ensure that cutting offset plate vertically cuts gel, avoid during cutting gel,
Cut offset plate run-off the straight and cause accidentally to cut target protein band the problem of；
3. gage is arranged on sliding slot inner wall, so as to which gel is accurately positioned by the gage being arranged on sliding slot inner wall
The location of Marker, and the scale value of the present position Marker is recorded, while cutting the offset plate alignment present position Marker
Scale value cut egg containing target to greatly improve to remove cutting gel again after making to cut the offset plate alignment position Marker
The accuracy of the PAGE gel of leukorrhea.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is the embodiments of the present invention, for those of ordinary skill in the art, without creative efforts, also
Other attached drawings can be obtained according to the attached drawing of offer.
Fig. 1 attached drawing is the structure application drawing before a kind of gel device for cutting cutting of the utility model；
Fig. 2 attached drawing is a kind of plastic cutting frame of gel device for cutting of the utility model and the structural schematic diagram for cutting offset plate；
Fig. 3 attached drawing is the structure application drawing in a kind of gel device for cutting cutting of the utility model.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
A kind of gel device for cutting provided by the utility model is accurately cut according to target protein molecular size range containing mesh
Transferring film is carried out again after marking the PAGE gel of protein band, is not only avoided waste PVDF/NC film, can also be avoided accidentally cutting target egg
Leukorrhea, to improve the success rate of experiment.
The utility model embodiment discloses a kind of gel device for cutting, including U-lag 1, plastic cutting frame 2, cuts offset plate 3 and amount
Ruler 4, plastic cutting frame 2 are slidably connected across the two sides of U-lag 1, and with the two sides of U-lag 1, cut offset plate 3 and are vertically arranged in and cut glue
It in frame 2, and is slidably connected with plastic cutting frame 2, the inside of U-lag 1 is arranged in gage 4.
In order to further optimize the above technical scheme, the groove wall top of 1 two sides of U-lag is provided with sliding slot 11, and sliding slot
11 two sides are respectively formed sliding slot outer wall 111 and sliding slot inner wall 112.
In order to further optimize the above technical scheme, gage 4 is arranged on sliding slot inner wall 112.
Root in order to further optimize the above technical scheme, it is sliding to be provided with the limit parallel with sliding slot 11 on sliding slot outer wall 111
Slot 12.
In order to further optimize the above technical scheme, plastic cutting frame 2 is made of slide plate 21 and crossbeam 22, the both ends point of crossbeam 22
Not with the top integrally connected of slide plate 21, and the bottom end of slide plate 21 is slidably connected with sliding slot 11.
In order to further optimize the above technical scheme, the bottom end of slide plate 21 is vertically and fixedly provided with traveller 211, traveller 211 and limit
Position sliding slot 12 is slidably connected, and traveller 211 passes through 12 threaded coupling nut 212 of limit sliding chutes.
In order to further optimize the above technical scheme, horizontally disposed crossbeam cuts glue groove 221 on crossbeam 22, cuts offset plate
3, which are vertically arranged in crossbeam, cuts in glue groove 221, and cuts glue groove 221 with crossbeam and be slidably connected.
In order to further optimize the above technical scheme, crossbeam 22 is provided with location hole 223 with the both ends for cutting offset plate 3, and
And the matching connection positioning pin 224 of location hole 223.
In order to further optimize the above technical scheme, it is provided with handle guide rail 213 along the vertical direction on crossbeam 22.
In order to further optimize the above technical scheme, the both ends for cutting offset plate 3 are formed with manipulation handle 31, and manipulate handle 31
It is slidably connected with handle guide rail 213.
Embodiment:
The utility model embodiment discloses a kind of gel device for cutting, including U-lag 1, plastic cutting frame 2, cuts offset plate 3 and amount
Ruler 4, plastic cutting frame 2 are slidably connected across the two sides of U-lag 1, and with the two sides of U-lag 1, and the groove wall top of 1 two sides of U-lag is equal
It is provided with sliding slot 11, and 11 two sides of sliding slot are respectively formed sliding slot outer wall 111 and sliding slot inner wall 112, gage 4 is arranged in sliding slot inner wall
On 112, the limit sliding chutes 12 parallel with sliding slot 11 are provided on sliding slot outer wall 111；
Plastic cutting frame 2 is made of slide plate 21 and crossbeam 22, the both ends of crossbeam 22 respectively with the top integrally connected of slide plate 21, and
The bottom end of slide plate 21 is slidably connected with sliding slot 11, and the bottom end of slide plate 21 is vertically and fixedly provided with traveller 211, traveller 211 and limit sliding chutes
12 are slidably connected, and traveller 211 passes through 12 threaded coupling nut 212 of limit sliding chutes, and sets in the horizontal direction on slide plate 21
It is equipped with handle guide rail 213, the both ends for cutting offset plate 3 are formed with manipulation handle 31, and manipulate handle 31 and the sliding of handle guide rail 213 company
It connects；Horizontally disposed crossbeam cuts glue groove 221 on crossbeam 22, cuts offset plate 3 and is vertically arranged in crossbeam and cuts in glue groove 221, and with
Crossbeam cuts glue groove 221 and is slidably connected, and crossbeam 22 and the both ends for cutting offset plate 3 are provided with location hole 223, and location hole 223
With connection positioning pin 224.
Operating procedure is as follows:
The complete S DS-PAGE gel of preparation is placed in placement box 6 by S1；
The placement box 6 for being placed with complete S DS-PAGE gel is pushed into from the open end of U-lag 1, and passes through " U " by S2
The box wall for disposing box 6 is fixed on the cell wall of 1 sealing end of U-lag and with the cell wall both ends of 1 two sides of U-lag by shape card 7 respectively
Together；
S3 is observed the position of Marker in PAGE gel by gage 4, reads and record in PAGE gel
The head end position scale value of Marker and the end-position scale value of Marker；
S4 pushes plastic cutting frame 2, the bottom end of slide plate 21 is made to be slidably connected with sliding slot 11, while traveller 211 and limit sliding chutes 12
It is slidably connected；
S5 is arranged in plastic cutting frame 2 by the observation of gage 4 and cuts the position of offset plate 3, if cut the position of offset plate 3 with
The head end position scale value of Marker is corresponding with the matching of the end-position scale value of Marker, then is slided by the fastening of nut 212
Column 211 keeps plastic cutting frame 2 fixed with respect to U-lag 1；
S6, one hand hold the manipulation handle 31 for cutting 3 one end of offset plate, and another hand-lifting falls the fixed positioning pin for cutting offset plate 3
224；
S7, one hand holds the manipulation handle 31 for cutting 3 one end of offset plate or both hands hold the manipulation handle for cutting 3 both ends of offset plate
31, thus control manipulation handle 31 slowly moved to bottom end along handle guide rail 213, while cut offset plate 3 along crossbeam cut glue groove 221 to
Bottom end is mobile, falls at gel Marker until cutting offset plate 3, and cuts off PAGE gel along gel Marker；
S8, circulation step S4-S7 are completed accurately along the task of Marker cutting PAGE gel；
S9, one hand hold the manipulation handle 31 for cutting 3 one end of offset plate, will cut offset plate 3 and lift along the direction far from gel, until
It cuts offset plate 3 and is in appropriate height, another hand is inserted into positioning pin 224 in location hole 223, completes the fixation to offset plate 3 is cut；
Each structure of the utility model is put in order stored on-site by S10.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so being described relatively simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments can be realized professional and technical personnel in the field or using originally practical new
Type.Various modifications to these embodiments will be readily apparent to those skilled in the art, and determine herein
The General Principle of justice can be realized in other embodiments without departing from the spirit or scope of the present utility model.Cause
This, the present invention will not be limited to the embodiments shown herein, and is to fit to and principles disclosed herein
The widest scope consistent with features of novelty.
Claims (10)
1. a kind of gel device for cutting characterized by comprising U-lag (1), cuts offset plate (3) and gage (4) at plastic cutting frame (2),
The plastic cutting frame (2) is slidably connected across the two sides of the U-lag (1), and with the two sides of the U-lag (1)；It is described to cut
Offset plate (3) is vertically arranged in the plastic cutting frame (2), and is slidably connected with the plastic cutting frame (2), and gage (4) setting exists
The inside of the U-lag (1).
2. a kind of gel device for cutting according to claim 1, which is characterized in that the cell wall top of U-lag (1) two sides
End is provided with sliding slot (11), and the sliding slot (11) two sides are respectively formed sliding slot outer wall (111) and sliding slot inner wall (112).
3. a kind of gel device for cutting according to claim 2, which is characterized in that the gage (4) is arranged in the cunning
On slot inner wall (112).
4. a kind of gel device for cutting according to claim 3, which is characterized in that be arranged on the sliding slot outer wall (111)
There are the limit sliding chutes parallel with the sliding slot (11) (12).
5. a kind of gel device for cutting according to claim 4, which is characterized in that the plastic cutting frame (2) is by slide plate (21)
It is formed with crossbeam (22), the both ends top integrally connected with the slide plate (21), and the slide plate respectively of the crossbeam (22)
(21) bottom end is slidably connected with the sliding slot (11).
6. a kind of gel device for cutting according to claim 5, which is characterized in that the bottom end of the slide plate (21) is vertically solid
Surely have traveller (211), the traveller (211) is slidably connected with the limit sliding chutes (12), and the traveller (211) passes through institute
State limit sliding chutes (12) threaded coupling nut (212).
7. a kind of gel device for cutting according to claim 5, which is characterized in that on the crossbeam (22) in the horizontal direction
Setting crossbeam is cut glue groove (221), it is described cut offset plate (3) and be vertically arranged in the crossbeam cut in glue groove (221), and with the crossbeam
Glue groove (221) is cut to be slidably connected.
8. a kind of gel device for cutting according to claim 5, which is characterized in that the crossbeam (22) cuts offset plate with described
(3) both ends are provided with location hole (223), and the location hole (223) matching connection positioning pin (224).
9. a kind of gel device for cutting according to claim 5, which is characterized in that on the crossbeam (22) along the vertical direction
It is provided with handle guide rail (213).
10. a kind of gel device for cutting according to claim 9, which is characterized in that the both ends for cutting offset plate (3) are formed
Have manipulation handle (31), and the manipulation handle (31) is slidably connected with the handle guide rail (213).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821434291.5U CN208887965U (en) | 2018-09-03 | 2018-09-03 | A kind of gel device for cutting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821434291.5U CN208887965U (en) | 2018-09-03 | 2018-09-03 | A kind of gel device for cutting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208887965U true CN208887965U (en) | 2019-05-21 |
Family
ID=66510928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821434291.5U Expired - Fee Related CN208887965U (en) | 2018-09-03 | 2018-09-03 | A kind of gel device for cutting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208887965U (en) |
-
2018
- 2018-09-03 CN CN201821434291.5U patent/CN208887965U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190521 Termination date: 20200903 |