CN208876494U - A kind of multichannel nerve signal optical fiber recording device - Google Patents
A kind of multichannel nerve signal optical fiber recording device Download PDFInfo
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- CN208876494U CN208876494U CN201820528416.4U CN201820528416U CN208876494U CN 208876494 U CN208876494 U CN 208876494U CN 201820528416 U CN201820528416 U CN 201820528416U CN 208876494 U CN208876494 U CN 208876494U
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Abstract
The utility model relates to a kind of multichannel nerve signal optical fiber recording devices, including control device, excitation light path device and fluorescent collecting device；The control device includes control computer and stepper motor；The excitation light path device includes laser, the first optical filter, two-phase color mirror, object lens and fiber coupling device；The fiber coupling device receives laser optical path, while reflected fluorescent light optical path；The fluorescent collecting device includes photoelectric sensor, lens, the second optical filter and two-phase color mirror；The control computer connection control stepper motor rotation；The control computer and photoelectric sensor communicate to connect；The optical circuit path of the step motor control laser；With such a structure, whole device structure is simplified, and saves device cost, while ensure that system stability, is reduced the influence of unstable factor in experiment, is reduced manufacturing cost.
Description
Technical field
The utility model relates to field more particularly to a kind of multichannel nerve signal optical fiber recording devices.
Background technique
The real-time sight of long time-histories is carried out to the neuron activity situation of the experimental animal related brain areas in specific behavior normal form
It surveys and record is the important method for parsing mammalian brain functional mechanism and coding characteristic.And in the multiple of composition neural circuit
Neururgic synchronous recording is carried out in brain area can provide the functional information of related brain areas in specific behavior in the level of circuit,
Important data reference and theories integration can be provided to further understand and treating related neurological disease.
Multichannel electro physiology is the traditional technology and most straightforward approach of record and reaction electrical activity of neurons information,
Have the advantages that individual cell level and high time precision.Since nervous activity electric signal is very faint, so that electrophysiological recording is real
Process complexity is tested, specific record efficiency is low, and is easy to be moved or experimental situation electromagnetic interference.Therefore, electricity is raw
Reason recording method is generally unsuitable for prolonged nervous activity record, and the behavior normal form that experimental animal is able to carry out also has very much
Limit.Calcium imaging is to convert fluorescence intensity signals for neuron activity electric signal using calcium sensitive fluorescent probe, to realize mind
A kind of new method recorded through movable optical imagery.Two-photon fluorescence microscope be currently used for recorded in body Ca2+ oscillations one
Kind comparative maturity and common technology, can be realized and synchronize note to the activity of hundreds and thousands of a neurons in same site
Record.High scattering and high-selenium corn feature due to biological tissue, this method are suitable for the tissue of superficial more at present, it is dynamic to be applicable in experiment
Object is also limited to the fixed behavior mouse in head.What is newly proposed in recent years utilizes optical fiber mediate calcium signal excitation and collection
Optical fiber records system, is to make up above method defect and fill up a kind of new technology of Related Research Domain blank.Optical fiber record side
Method mediates the collection for realizing deep brain calcium fluorescence signal, the good flexibility of optical fiber and electromagnetic wave shielding maximum limit using implantation optical fiber
Reduce influence of the record system to experimental animal behavior normal form on degree, so that being may be implemented with this method in a variety of behaviors
The activity for moveing freely the specific brain area neuron of animal is monitored and recorded for a long time under normal form.On this basis, lead to
The method that light splitting increases multidigit point embedded fibers in system detection optical path and neural circuit is crossed, can also realize a certain functional neurosurgery
Multiple neururgic synchronous recordings of brain area in circuit, provide more abundant brain function information for researcher.But it is existing
It is divided multichannel extended method heavy workload and lacks flexibility, as the increase system authority of number of active lanes is excessively huge.Consider
To the diversity and complexity of mammalian brain neural circuit, it is necessary to propose a kind of light for capableing of flexible expansion number of active lanes
Fine network system realization.
Utility model content
The technical problems to be solved in the utility model is to design a kind of multichannel nerve signal optical fiber recording device, is solved existing
Has light splitting multi-channel optical fibre record system for the multidigit point Ca2+ oscillations synchronous recording in neural circuit research, there are number of active lanes
Can not flexible expansion or system extension based on system light path transformation the problem of, it is complicated to be unable to satisfy brain function research problem multiplicity
Feature.
In order to solve the above technical problems, the utility model provides a kind of multichannel nerve signal optical fiber recording device, including
Control device, excitation light path device and fluorescent collecting device；The control device includes control computer and stepper motor；It is described
Excitation light path device includes laser, the first optical filter, two-phase color mirror, object lens and fiber coupling device；The fiber coupling dress
Set reception laser optical path, while reflected fluorescent light optical path；The fluorescent collecting device includes photoelectric sensor, lens, the second optical filtering
Piece and two-phase color mirror；The control computer connection control stepper motor rotation；The control computer and photoelectric sensor are logical
Letter connection；The optical circuit path of the step motor control laser；The optical filter is located on the illumination path path of laser；
The two-phase color mirror is located on the optical circuit path of the first filter transmission；The object lens are located at two-phase color mirror transmitted light path path
On；The fiber coupling device is located on object lens transmitted light path path；The object lens are located at fiber coupling device fluorescent reflection
On Route Routes；Second optical filter is located on two-phase color mirror fluorescent reflection optical circuit path；The lens are located at second and filter
Piece transmits on fluorescence light path path；The photoreceptor of the photoelectric sensor is located on lens transmission fluorescence light path path.
Further, the laser is semiconductor laser.Since this programme needs for exciting light to be accurately coupled into
Multifiber realizes the signal acquisition of multichannel, while needing light intensity relatively stable, therefore is completed using semiconductor laser.
Further, the fiber coupling device is installed at experimental animal nerve cell to be measured.
It further, further include three optical fiber on the fiber coupling device, it is inswept that three optical fiber are fixed on excitation light path
Path institute in the plane.
Further, the two-phase color mirror and fluorescent reflection optical path are in 45° angle.Two-phase color mirror is put is in return fluorescence
45° angle setting can then carry out approximate 90 ° of reflection to the fluorescence signal of wavelength, then carry out wavelength selection by optical filter again
(likewise, the fluorescent signals wavelengths that different calcon-carboxylic acids returns are different, selected two-phase color mirror and optical filter are also different
Sample), then collimated by lens on light road, which is finally acquired by the photoelectric sensor of end, thus real
Show and the fluorescence signal for characterizing cellular activity is converted into digital signal, has passed control device back and be acquired and handle.
Further, first optical filter between laser at a distance from be 3-5cm；First optical filter and two-phase color
Distance between mirror is 3-5cm；Distance between the two-phase color mirror and object lens is 5-8cm；Between the fiber coupling device and object lens
Distance be 5mm；The distance between second optical filter and two-phase color mirror are 5-8cm；The lens and the second optical filter it
Between distance be 1-2cm；The distance between the photoelectric sensor and lens are 1-2cm.
The utility model has the beneficial effects that with such a structure, whole device structure is simplified, device cost is saved,
While ensure that system stability, reduces the influence of unstable factor in experiment, reduce manufacturing cost.
Detailed description of the invention
Specific embodiment of the present utility model is furtherd elucidate with reference to the accompanying drawing.
Fig. 1 is the internal structure chart of the multichannel nerve signal optical fiber recording device of the utility model；
Fig. 2 is the schematic diagram of the data collection point of the multichannel nerve signal optical fiber recording device of the utility model.
Specific embodiment
In conjunction with Fig. 1 to Fig. 2, the utility model provides a kind of multichannel nerve signal optical fiber recording device, a kind of multichannel mind
Through signal optical fibre recording device, including control device, excitation light path device and fluorescent collecting device；The control device includes control
Computer and stepper motor processed；The excitation light path device includes laser, the first optical filter, two-phase color mirror, object lens and optical fiber
Coupling device；The fiber coupling device receives laser optical path, while reflected fluorescent light optical path；The fluorescent collecting device includes light
Electric transducer, lens, the second optical filter and two-phase color mirror；The control computer connection control stepper motor rotation；The control
Computer processed and photoelectric sensor communicate to connect；The optical circuit path of the step motor control laser；The optical filter is located at
On the illumination path path of laser；The two-phase color mirror is located on the optical circuit path of the first filter transmission；The object lens position
In on two-phase color mirror transmitted light path path；The fiber coupling device is located on object lens transmitted light path path；The object lens are located at
On the Route Routes of fiber coupling device fluorescent reflection；Second optical filter is located at two-phase color mirror fluorescent reflection optical circuit path
On；The lens are located on the second filter transmission fluorescence light path path；It is saturating that the photoreceptor of the photoelectric sensor is located at lens
It penetrates on fluorescence light path path.
Further, the laser is semiconductor laser.
Further, the fiber coupling device is installed at experimental animal nerve cell to be measured.
It further, further include three optical fiber on the fiber coupling device, it is inswept that three optical fiber are fixed on excitation light path
Path institute in the plane.
Further, the two-phase color mirror and fluorescent reflection optical path are in 45° angle.
Further, first optical filter between laser at a distance from be 3-5cm；First optical filter and two-phase color
Distance between mirror is 3-5cm；Distance between the two-phase color mirror and object lens is 5-8cm；Between the fiber coupling device and object lens
Distance be 5mm；The distance between second optical filter and two-phase color mirror are 5-8cm；The lens and the second optical filter it
Between distance be 1-2cm；The distance between the photoelectric sensor and lens are 1-2cm.
The course of work:
Laser is placed on levelling bench first, disposes optical filter, the 3- after optical filter at 3-5cm before light-emitting window
Two-phase color mirror is disposed at 5cm, two-phase color mirror angle and optical filter are substantially in that 45° angle is placed, and are disposed at 5-8cm after two-phase color mirror
Object lens, laser light-emitting window, optical filter, two-phase color mirror center and object lens center should be in same optical axis at this time.Same flat
3 optical fiber are fixed in placement on another levelling bench in face, and fiber distance object lens about 5mm, 3 optical fiber spacing 1mm are horizontal positioned,
It is parallel with optical axis simultaneously.The optical filter of fluorescent collecting optical path is positioned approximately in after two-phase color mirror at 5-8cm, lens distance optical filter
1-2cm, photoelectric sensor is apart from lens 1-2cm.
The present embodiment is a kind of time-sharing multiplex light source and acquisition equipment to realize multichannel nervous activity fluorescent signals
The device of detection and record, by the way that excitation light source is coupled into different optical fiber in different time, then within the corresponding time
The fluorescent signals of channel return are acquired, to reach the scheme of multiple recording.
It is broadly divided into excitation light path device, fluorescent collecting device and control device three parts.Excitation light path is used and is partly led
Body laser is as light source, by exciting light coupled into optical fibres；The characterization nervous activity that fluorescent collecting optical path will be returned by neuron
Fluorescent signals collect, and be converted to digital signal and be transmitted to control device and be acquired；Control device is then used to control
Exciting light processed is periodically deflected in horizontal position, exciting light is periodically coupled in optical fiber in different time, together
When periodically signal is acquired, the final signal acquisition scheme for realizing multichannel.
Excitation light path device: the neururgic week fluorescent letter of the characterization that exciting light is used to that calcon-carboxylic acid is excited to generate
Number, the signal acquisition of multichannel is realized since this programme needs for exciting light to be accurately coupled into multifiber, while needing light
It is strong relatively stable, therefore completed using semiconductor laser.The exciting light of semiconductor laser first passes through optical filter and completes to wavelength
Selection (according to the different indicator, excitation wavelength plurality of optional, optical filter is also), utilized after through two-phase color mirror
Object lens coupled into optical fibres coupling device, the other end of fiber coupling device are embedded at experimental animal nerve cell, calcium instruction
After agent receives exciting light, side can generate the fluorescence signal of respective strengths, fluorescence letter according to the active state of Current neural cell
Number two-phase color mirror is back to again by same root fiber coupling device and object lens.
Fluorescent collecting device: the fluorescence signal generated by calcon-carboxylic acid is back to two by fiber coupling device and object lens
Phase Look mirror, the two-phase color mirror put and return to fluorescence in 45° angle setting, then approximate 90 ° can be carried out to the fluorescence signal of wavelength
Then reflection carries out wavelength selection (likewise, the fluorescent signals wavelengths that different calcon-carboxylic acids returns are not by optical filter again
Together, selected two-phase color mirror and optical filter are also different), then collimated by lens on light road, the fluorescence signal is final
It is acquired by the photoelectric sensor of end, the fluorescence signal for characterizing cellular activity is converted into digital signal to realize,
Control device is passed back to be acquired and handle.
Control device: control device completes two parts task, first is that the light direction to laser controls, thus will
Exciting light is periodically coupled into different optical fiber in different time；Second is that the fluorescence passed back by different optical fiber is carried out periodically
Acquisition.
Control device is the part of the Multi channel most critical, controls two parts by stepper motor and computer and forms.
Excitation light path is placed on platform, while being carried out periodically using computer-controlled stepper motor band moving platform in horizontal position
Rule rotation, such exciting light will carry out periodic scan in a certain range in the horizontal direction, by multifiber
It is fixed on the plane of scanning motion, exciting light will be periodically coupled in each optical fiber with certain rule, to the channel
Nerve cell carry out the excitation of signal, while other channels will not generate fluorescence signal due to not having exciting light.
On the one hand computer controls the rotation of stepper motor, while the signal that processing is passed back by photoelectric sensor.To scheme
For, when exciting light coupled into optical fibres 1, collected Modulation recognition this moment is the data in channel 1 by computer, works as exciting light
It is the data in channel 2 by the Modulation recognition at the moment, when exciting light passes through optical fiber 3, by the letter at the moment when by optical fiber 2
The data in channel 3 number are classified as, and so on.By changing the velocity of rotation of stepper motor and the arrangement and computer of optical fiber
Capture setting, the signal acquisition of more number of active lanes may be implemented in this method.
Elaborate many details in the above description in order to fully understand the utility model.But above description
It is only the preferred embodiment of the utility model, the utility model can be come with being much different from other way described herein
Implement, therefore the utility model is not limited by specific implementation disclosed above.Any those skilled in the art exist simultaneously
It does not depart under technical solutions of the utility model ambit, it is all practical new to this using the methods and technical content of the disclosure above
Type technical solution makes many possible changes and modifications or equivalent example modified to equivalent change.It is all without departing from this
The content of utility model technical solution any is simply repaired according to the technical essence of the utility model is made to the above embodiment
Change, equivalent variations and modification, in the range of still falling within technical solutions of the utility model protection.
Claims (7)
1. a kind of multichannel nerve signal optical fiber recording device, it is characterised in that: including control device, excitation light path device and fluorescence
Acquisition device；The control device includes control computer and stepper motor；The excitation light path device includes laser, first
Optical filter, two-phase color mirror, object lens and fiber coupling device；The fiber coupling device receives laser optical path, while reflected fluorescent light
Optical path；The fluorescent collecting device includes photoelectric sensor, lens, the second optical filter and two-phase color mirror；The control computer
Connection control stepper motor rotation；The control computer and photoelectric sensor communicate to connect；The step motor control laser
The optical circuit path of device；The optical filter is located on the illumination path path of laser；The two-phase color mirror is located at the first optical filter
On the optical circuit path of transmission；The object lens are located on two-phase color mirror transmitted light path path；The fiber coupling device is located at object lens
On transmitted light path path；The object lens are located on the Route Routes of fiber coupling device fluorescent reflection；Second optical filter position
In on two-phase color mirror fluorescent reflection optical circuit path；The lens are located on the second filter transmission fluorescence light path path；The light
The photoreceptor of electric transducer is located on lens transmission fluorescence light path path.
2. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: the laser is partly to lead
Body laser.
3. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: the fiber coupling device
It is installed at experimental animal nerve cell to be measured.
4. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: the fiber coupling device
On further include three optical fiber, three optical fiber are fixed on the inswept path institute of excitation light path in the plane.
5. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: the control computer control
Stepper motor processed carries out flat scanning reciprocating rotation, scan period 40ms within the scope of 20 °.
6. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: the two-phase color mirror with it is glimmering
Light reflected light path is in 45° angle.
7. multichannel nerve signal optical fiber recording device according to claim 1, it is characterised in that: first optical filter with
Distance between laser is 3-5cm；First optical filter between two-phase color mirror at a distance from be 3-5cm；The two-phase color mirror and
Distance between object lens is 5-8cm；The fiber coupling device between object lens at a distance from be 5mm；Second optical filter and two-phase
The distance between Look mirror is 5-8cm；The distance between the lens and the second optical filter are 1-2cm；The photoelectric sensor with
The distance between lens are 1-2cm.
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| CN201820528416.4U CN208876494U (en) | 2018-04-14 | 2018-04-14 | A kind of multichannel nerve signal optical fiber recording device |
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| CN201820528416.4U CN208876494U (en) | 2018-04-14 | 2018-04-14 | A kind of multichannel nerve signal optical fiber recording device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115316960A (en) * | 2022-10-13 | 2022-11-11 | 浙江大学医学中心(余杭) | Brain nerve activity regulation and control and brain information synchronous reading system |
| CN115969370A (en) * | 2022-12-30 | 2023-04-18 | 深圳市瑞沃德生命科技有限公司 | Optical fiber recording apparatus |
-
2018
- 2018-04-14 CN CN201820528416.4U patent/CN208876494U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115316960A (en) * | 2022-10-13 | 2022-11-11 | 浙江大学医学中心(余杭) | Brain nerve activity regulation and control and brain information synchronous reading system |
| CN115316960B (en) * | 2022-10-13 | 2023-03-31 | 浙江大学医学中心(余杭) | Brain nerve activity regulation and control and brain information synchronous reading system |
| CN115969370A (en) * | 2022-12-30 | 2023-04-18 | 深圳市瑞沃德生命科技有限公司 | Optical fiber recording apparatus |
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