CN208857310U - A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection - Google Patents

A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection Download PDF

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Publication number
CN208857310U
CN208857310U CN201821428408.9U CN201821428408U CN208857310U CN 208857310 U CN208857310 U CN 208857310U CN 201821428408 U CN201821428408 U CN 201821428408U CN 208857310 U CN208857310 U CN 208857310U
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test tube
frame
support frame
bottom plate
blanking
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CN201821428408.9U
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凡玉芳
许高涛
周炜
夏兵兵
王蓉
王亚男
徐慕珍
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Wuhu Phil Biological Products Industry Research Institute Co Ltd
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Wuhu Phil Biological Products Industry Research Institute Co Ltd
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Abstract

The utility model relates to Biological Detection fields, in particular to a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection, it include fixation kit, buffer blanking component, test tube rocking frame and for drive test tube rocking frame swing driving assembly, the fixation kit includes a bottom plate, one blanking support frame, two reel cage support rods and four support legs, the bottom plate is rectangular plate-like structure, the two sides on test tube rocking frame top are separately provided for the pendant being suspended on axis extension bar, test tube rocking frame is suspended on two axis extension bars by pendant, the blanking support frame is mounted on the side on bottom plate top, buffer blanking component is mounted on the top of blanking support frame, test tube rocking frame is driven to be rocked by driving assembly, so that the collision of buffer and cell strain, increase dilution effect, lead to again The mode of the screw blanking of buffer blanking component is crossed, so that buffer blanking is more accurate.

Description

A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection
Technical field
The utility model relates to Biological Detection field, in particular to a kind of stabilization of sheep interferon immunopotentiator detection Transfection cell strain dilution device.
Background technique
Cell strain: being obtained from primary culture or cell line by back-and-forth method or clone forming method with special nature or The culture of marker is known as cell strain, that is to say, that and cell strain is to be separately cultured or with unicellular by the method for screening, by The cell mass that unicellular proliferation is formed.The special nature or mark of cell strain must exist always during entire culture.
Primary culture is cell line after being passed on for the first time successfully, by the cell generation being originally present in primary culture System is formed.It if cannot continue to pass on or passage number is limited, can be described as finite cell lines, such as can continuously cultivate, then claim For continuous cell line, culture 50 is more than generation and infinitely culture is gone down.So cell strain be by back-and-forth method or clone forming method from The culture cell with special nature or mark obtained in primary culture or cell line.For culture algebra, it can cultivate To 40-50 generation.The special nature or mark of cell strain must exist always during entire culture.If cannot continue passage or Passage number is limited, referred to as limited cellular strain;If can be with continuous passage, referred to as successive cell strain.For human tumor cell, In vitro culture more than half a year, stablize by growth, and continuous passage is that can be described as continuity strain or be.
Cell strain is to need to add buffer to be diluted, but connect due to often there is buffer with cell strain in culture Touching is not enough to add or add with buffer causes cell strain culture dead situation occur less, is unable to get effective control System, it is therefore necessary to design a kind of dilution device control.
Utility model content
The purpose of this utility model is that in view of the deficiencies of the prior art, a kind of sheep interferon Immune-enhancing effect detection is provided Stable transfected cells strain dilution device drives test tube rocking frame to be rocked, so that buffer and cell strain by driving assembly Collision, increase dilution effect, then by way of the screw blanking of buffer blanking component, so that buffer blanking is more Accurately.
To solve the above problems, the utility model the following technical schemes are provided:
A kind of stable transfected cells strain dilution device of sheep interferon Immune-enhancing effect detection, includes fixation kit, buffering Liquid blanking component, test tube rocking frame and the driving assembly for driving test tube rocking frame to swing, the fixation kit includes one A bottom plate, a blanking support frame, two reel cage support rods and four support legs, the bottom plate be rectangular plate-like structure, four The support leg is mounted on four angles of bottom plate bottom end in the arrangement of square shape, and the driving component is mounted on the interposition of bottom plate bottom end It sets, two reel cage support rod states in a vertical shape are separately positioned on the two sides on bottom plate top, and the top of each reel cage support rod is all provided with It is equipped with the axis extension bar extended towards base plate center line direction, the two sides on test tube rocking frame top are separately provided for being suspended on axis Pendant on extension bar, test tube rocking frame are suspended on two axis extension bars by pendant, and the blanking support frame is mounted on base top The side at end, buffer blanking component are mounted on the top of blanking support frame.
Further, the bottom end of four support legs is provided with for adsorbing sucker on the table, and sucker is set It sets in the bottom end of support leg.
Further, the driving component includes shell, electromagnet, controller, control button and power interface, described Electromagnet and controller are installed in the bottom end of bottom plate, and shell is mounted on the bottom end of bottom plate and covers on controller and electromagnet Inside, the power connection are mounted on the side of shell, and the control button is mounted on the top of bottom plate, the test tube rocking frame Bottom end be additionally provided with the anode magnet block for cooperating electromagnet, the electromagnet, control button and power interface with control Device processed is electrically connected, and is coaxially set between test tube rocking frame, anode magnet block and electromagnet three.
Further, the test tube rocking frame includes the first connection frame, the second connection frame, test tube sleeve, test tube pedestal And fixed plate, first connection frame and the second connection frame are inverted L-shaped structure, the first connection frame and the second connection frame Horizontal part is oppositely arranged and the outer wall of test tube sleeve is connect with the end face of the first connection frame and the second connection frame horizontal part, and two A pendant is separately mounted to the top of the first connection frame and the second connection frame horizontal part, and fixed plate, which is in a horizontal state, is mounted on first The bottom end of connection frame and the second connection frame vertical portion, test tube pedestal are mounted on the middle position on fixed plate top, the test tube sleeve Cylinder is cirque structure, and the two sides of the inner wall of test tube sleeve are symmetrical arranged two pieces of rubber mounts.
Further, the blanking support frame includes the first support frame, the second support frame, the first telescopic rod, second stretches Contracting bar and loading plate, first support frame and the second support frame are hollow tubular structures, the first support frame and the second support frame State setting in a vertical shape is mounted on the side on bottom plate top and is arranged in parallel between two kinds of the first support frame and the second support frame, First telescopic rod and the second telescopic rod are inserted into respectively in the first support frame and the second support frame, the first support frame and the second support frame The side on top is provided with the clamping screw for locking the first telescopic rod and the second telescopic rod, and loading plate, which is in a horizontal state, to be set It sets in the first telescopic rod and the second telescopic rod top end and loading plate is located at the top of bottom plate.
Further, the buffer blanking component includes barrel, threaded rod, crank, rubber push top and fixes bracket, The barrel is the circular barrel-like structure structure of top end opening, and the bottom end of barrel is provided with the feed opening for buffer blanking, The barrel state in a vertical shape passes through loading plate and the feed opening of barrel barrel faces the test tube in plumbness, barrel It is fixedly connected with loading plate, rubber is had a haircut to be connect with the bottom end axis of threaded rod, and fixed bracket is inverted L-shaped structure, fixed bracket The side of barrel is set and fixed bracket is fixedly connected with the top of loading plate, it is solid that threaded rod state in a vertical shape passes through setting The inside of fixed rack horizontal part insertion barrel and threaded rod and fixed bracket are threadedly engaged, outer wall that rubber is had a haircut and barrel The top of threaded rod is arranged in inner wall interference fit, the crank.
The utility model has the advantages that
A kind of stable transfected cells strain dilution device of sheep interferon Immune-enhancing effect detection of the utility model, passes through driving Component driver test tube rocking frame is rocked, so that the collision of buffer and cell strain, increases dilution effect, then pass through buffer Test tube equipped with cell strain is placed on examination so that buffer blanking is more accurate by the mode of the screw blanking of blanking component Pipe rocking frame, to suitable buffer is added in test tube, is opening electromagnet by buffer blanking component, so that test tube shakes Frame rocks, and observes invisible spectro reaction, carries out addition buffer gradually.
Detailed description of the invention
Fig. 1 is the schematic perspective view one of the utility model;
Fig. 2 is the schematic perspective view two of the utility model;
Fig. 3 is the stereogram exploded view of the utility model test tube rocking frame;
Fig. 4 is the utility model fixation kit volume schematic perspective view;
Fig. 5 is the stereogram exploded view of the utility model driving assembly;
Fig. 6 is the stereogram exploded view of the utility model blanking support frame;
Fig. 7 is the stereogram exploded view of the utility model buffer blanking component;
Description of symbols: fixation kit 1, bottom plate 11, blanking support frame 12, the first support frame 121, the second support frame 122, the first telescopic rod 123, the second telescopic rod 124, loading plate 125, clamping screw 126, reel cage support rod 13, support leg 14, Sucker 15, buffer blanking component 2, barrel 21, threaded rod 22, crank 23, rubber push top 24, fixed bracket 25, test tube shake Frame 3, the first connection frame 31, the second connection frame 32, test tube sleeve 33, test tube pedestal 34, rubber mount 35, fixed plate 36, pendant 37, driving assembly 4, shell 41, electromagnet 42, controller 43, control button 44, power interface 45, anode magnet block 46.
Specific embodiment
With reference to the accompanying drawings of the specification and embodiment, specific embodiment of the utility model is described in further detail:
A kind of stable transfected cells strain dilution device of sheep interferon Immune-enhancing effect detection, packet shown in referring to Figure 1 to Figure 7 Include fixation kit 1, buffer blanking component 2, test tube rocking frame 3 and the driving assembly for driving test tube rocking frame 3 to swing 4, the fixation kit 1 includes the reel cage support rod 13 of blanking support frame 12, two of bottom plate 11, one and four support legs 14, the bottom plate 11 is rectangular plate-like structure, and four support legs 14 are mounted on four of 11 bottom end of bottom plate in the arrangement of square shape Angle, the driving component 4 are mounted on the middle position of 11 bottom end of bottom plate, and two states in a vertical shape of reel cage support rod 13 are respectively set In the two sides on 11 top of bottom plate, the top of each reel cage support rod 13 is provided with the axis extended towards 11 centerline direction of bottom plate Extension bar, the two sides on 3 top of test tube rocking frame are separately provided for the pendant 37 being suspended on axis extension bar, and test tube rocking frame 3 is logical It crosses pendant 37 to be suspended on two axis extension bars, the blanking support frame 12 is mounted on the side on 11 top of bottom plate, buffer blanking Component 2 is mounted on the top of blanking support frame 12.
The bottom end of four support legs 14 is provided with for adsorbing sucker 15 on the table, and the setting of sucker 15 exists The bottom end of support leg 14.Sucker 15 is set in 14 bottom end of support leg, dilution device is integrally adsorbed on by workbench by sucker 15 Table top so that dilution device is relatively stable.
The driving component 4 includes shell 41, electromagnet 42, controller 43, control button 44 and power interface 45, institute It states electromagnet 42 and controller 43 is installed in the bottom end of bottom plate 11, shell 41 is mounted on the bottom end of bottom plate 11 and by controller 43 cover on inside with electromagnet 42, and the power connection is mounted on the side of shell 41, and the control button 44 is mounted on bottom plate 11 top, the bottom end of the test tube rocking frame 3 are additionally provided with the anode magnet block 46 for cooperating electromagnet 42, the electromagnetism Iron 42, control button 44 and power interface 45 are electrically connected with controller 43, test tube rocking frame 3, anode magnet block 46 and electricity It is coaxially set between magnet three.By the cooperation of electromagnet 42 and anode magnet block 46, so that test tube rocking frame 3 shakes, It should be noted that passing through the control of control button 44, the outbound course for controlling electric current makes electromagnet 42 in anode and cathode Between convert, by principle of same-sex repulsion, so that the anode magnet block 46 of 3 bottom end of test tube rocking frame drives test tube rocking frame 3 to put It is dynamic, by the principle that there is a natural attraction between the sexes, so as to drive test tube rocking frame 3 to fall in vertical for the anode magnet block 46 of 3 bottom end of test tube rocking frame Straight state.
The test tube rocking frame 3 includes the first connection frame 31, the second connection frame 32, test tube sleeve 33, test tube pedestal 34 With fixed plate 36, first connection frame 31 and the second connection frame 32 are inverted L-shaped structure, the first connection frame 31 and second The horizontal part of connection frame 32 is oppositely arranged and 32 level of the outer wall of test tube sleeve 33 and the first connection frame 31 and the second connection frame The end face in portion connects, and two pendants 37 are separately mounted to the top of 32 horizontal part of the first connection frame 31 and the second connection frame, Gu Fixed board 36, which is in a horizontal state, is mounted on the bottom end of 32 vertical portion of the first connection frame 31 and the second connection frame, and test tube pedestal 34 is mounted on The middle position on 36 top of fixed plate, the test tube sleeve 33 are cirque structure, and the two sides of the inner wall of test tube sleeve 33 are symmetrical Two pieces of rubber mounts 35 are set.By pendant 37 by 3 integral hanging of test tube rocking frame on fixation kit 1, then pass through electromagnet So that test tube rocking frame 3 is swung, so that invisible spectro cell strain is adequately collided with buffer, make dilution effect more significant, The bottom of test tube is placed on test tube pedestal 34 by test tube insertion test tube sleeve 33 fixes test tube.
The blanking support frame 12 includes the first support frame 121, the second support frame 122, the first telescopic rod 123, second Telescopic rod 124 and loading plate 125, first support frame 121 and the second support frame 122 are hollow tubular structures, the first support Frame 121 and the state setting in a vertical shape of the second support frame 122 are mounted on the side on 11 top of bottom plate and the first support frame 121 and the It is arranged in parallel between two 122 two kinds of support frames, the first telescopic rod 123 and the second telescopic rod 124 are inserted into the first support frame 121 respectively In the second support frame 122, the side on 122 top of the first support frame 121 and the second support frame is provided with for locking first The clamping screw 126 of telescopic rod 123 and the second telescopic rod 124, loading plate 125, which is in a horizontal state, to be arranged in the first telescopic rod 123 With 124 top of the second telescopic rod and loading plate 125 is located at the top of bottom plate 11.By locking or unclamping clamping screw 126 The height of loading plate 125 is adjusted, in order to changing different size of buffer blanking component 2.
The buffer blanking component 2 includes barrel 21, threaded rod 22, crank 23, rubber push top 24 and fixed bracket 25, the barrel 21 is the circular barrel-like structure structure of top end opening, and the bottom end of barrel 21 is provided with for buffer blanking Feed opening, the barrel 21 state in a vertical shape passes through loading plate 125 and the feed opening of 21 barrel 21 of barrel is faced in vertical The test tube of straight state, barrel 21 are fixedly connected with loading plate 125, and rubber push top 24 and the bottom end axis of threaded rod 22 connect, fixed branch Frame 25 is inverted L-shaped structure, and the top of the side of barrel 21 and fixed bracket 25 and loading plate 125 is arranged in fixed bracket 25 End is fixedly connected, and the state in a vertical shape of threaded rod 22 passes through the inside that fixed 25 horizontal part of bracket insertion barrel 21 is arranged and screw thread Bar 22 is threadedly engaged with fixed bracket 25, and the outer wall of rubber push top 24 and the inner wall of barrel 21 are interference fitted, and the crank 23 is set It sets on the top of threaded rod 22.By shaking crank 23, so that threaded rod 22 is the active force that fixed bracket 25 is threadedly engaged Under, risen or declined, rubber push top 24 is pushed when threaded rod 22 declines, rubber push top 24 pushes the buffering in barrel 21 Liquid enters in test tube, when needing to add buffer, by shaking crank 23 rubber push top 24 is climbed away in barrel 21 It is added.
Working principle: being placed on test tube rocking frame 3 for the test tube equipped with cell strain, right by buffer blanking component 2 The suitable buffer of addition in test tube, opens electromagnet, so that test tube rocking frame 3 rocks, observes invisible spectro reaction, carry out by The addition buffer of step.
The above descriptions are merely preferred embodiments of the present invention, not makees to the technical scope of the utility model Any restrictions out, thus it is any trickle amendment made by the above technical examples according to the technical essence of the present invention, equivalent Variation and modification, in the range of still falling within the technical solution of the utility model.

Claims (6)

1. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection, it is characterised in that: include solid Determine component (1), buffer blanking component (2), test tube rocking frame (3) and the driving group for driving test tube rocking frame (3) to swing Part (4), the fixation kit (1) include a bottom plate (11), a blanking support frame (12), two reel cage support rods (13) With four support legs (14), the bottom plate (11) is rectangular plate-like structure, and four support legs (14) are in the arrangement installation of square shape At four angles of bottom plate (11) bottom end, the driving component (4) is mounted on the middle position of bottom plate (11) bottom end, two reel cage branch Strut (13) state in a vertical shape is separately positioned on the two sides on bottom plate (11) top, and the top of each reel cage support rod (13) is respectively provided with There is the axis extension bar extended towards bottom plate (11) centerline direction, the two sides on test tube rocking frame (3) top are separately provided for hanging The pendant (37) on axis extension bar is hung over, test tube rocking frame (3) is suspended on two axis extension bars by pendant (37), the blanking branch Support (12) is mounted on the side on bottom plate (11) top, and buffer blanking component (2) is mounted on the top of blanking support frame (12).
2. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection according to claim 1, It is characterized by: the bottom end of four support legs (14) is provided with for adsorbing sucker (15) on the table, sucker (15) it is arranged in the bottom end of support leg (14).
3. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection according to claim 2, It is characterized by: the driving component (4) include shell (41), electromagnet (42), controller (43), control button (44) and Power interface (45), the electromagnet (42) and controller (43) are installed in the bottom end of bottom plate (11), and shell (41) is mounted on The bottom end of bottom plate (11) and controller (43) and electromagnet (42) are covered on into inside, the power interface is mounted on shell (41) Side, the control button (44) is mounted on the top of bottom plate (11), and the bottom end of the test tube rocking frame (3) is additionally provided with use In the anode magnet block (46) of cooperation electromagnet (42), the electromagnet (42), control button (44) and power interface (45) are equal It is electrically connected with controller (43), is coaxially set between test tube rocking frame (3), anode magnet block (46) and battery iron three.
4. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection according to claim 3, It is characterized by: the test tube rocking frame (3) include the first connection frame (31), the second connection frame (32), test tube sleeve (33), Test tube pedestal (34) and fixed plate (36), first connection frame (31) and the second connection frame (32) are inverted L-shaped structure, The horizontal part of first connection frame (31) and the second connection frame (32) is oppositely arranged and the outer wall of test tube sleeve (33) and first connects It connects frame (31) to connect with the end face of the second connection frame (32) horizontal part, two pendants (37) are separately mounted to the first connection frame (31) With the top of the second connection frame (32) horizontal part, fixed plate (36), which is in a horizontal state, is mounted on the first connection frame (31) and the second company The bottom end of frame (32) vertical portion is connect, test tube pedestal (34) is mounted on the middle position on fixed plate (36) top, the test tube sleeve It (33) is cirque structure, the two sides of the inner wall of test tube sleeve (33) are symmetrical arranged two pieces of rubber mounts (35).
5. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection according to claim 4, It is characterized by: the blanking support frame (12) includes the first support frame (121), the second support frame (122), the first telescopic rod (123), the second telescopic rod (124) and loading plate (125), first support frame (121) and the second support frame (122) are hollow Tubular structure, the first support frame (121) and the second support frame (122) state setting in a vertical shape are mounted on the one of bottom plate (11) top It is arranged in parallel between side and the first support frame (121) and (122) two kinds of the second support frame, the first telescopic rod (123) and second Telescopic rod (124) is inserted into respectively in the first support frame (121) and the second support frame (122), the first support frame (121) and second The side on support (122) top is provided with the clamping screw for locking the first telescopic rod (123) and the second telescopic rod (124) (126), loading plate (125), which is in a horizontal state, is arranged on the first telescopic rod (123) and the second telescopic rod (124) top and carries Plate (125) is located at the top of bottom plate (11).
6. a kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection according to claim 5, It is characterized by: the buffer blanking component (2) includes barrel (21), threaded rod (22), crank (23), rubber push top (24) and fixed bracket (25), the barrel (21) is the circular barrel-like structure structure of top end opening, and the bottom end of barrel (21) is set It is equipped with the feed opening for buffer blanking, the barrel (21) state in a vertical shape passes through loading plate (125) and barrel (21) The feed opening of barrel (21) faces the test tube in plumbness, and barrel (21) is fixedly connected with loading plate (125), and rubber pushes away Head (24) and the bottom end axis of threaded rod (22) connect, and fixed bracket (25) are inverted L-shaped structure, and fixed bracket (25) setting is being expected The side of cylinder (21) and fixed bracket (25) are fixedly connected with the top of loading plate (125), threaded rod (22) state in a vertical shape Across the inside and threaded rod (22) and fixed bracket (25) screw thread that fixed bracket (25) horizontal part insertion barrel (21) is arranged Cooperation, the outer wall of rubber push top (24) and the inner wall of barrel (21) are interference fitted, and the crank (23) is arranged in threaded rod (22) Top.
CN201821428408.9U 2018-08-31 2018-08-31 A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection Active CN208857310U (en)

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CN201821428408.9U CN208857310U (en) 2018-08-31 2018-08-31 A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection

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CN201821428408.9U CN208857310U (en) 2018-08-31 2018-08-31 A kind of stable transfected cells strain dilution device of sheep interferon immunopotentiator detection

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113289526A (en) * 2021-04-22 2021-08-24 安徽福贸生物科技有限公司 New coronavirus detect reagent box and use dilution equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113289526A (en) * 2021-04-22 2021-08-24 安徽福贸生物科技有限公司 New coronavirus detect reagent box and use dilution equipment
CN113289526B (en) * 2021-04-22 2024-03-05 安徽福贸生物科技有限公司 Dilution equipment for novel coronavirus detection kit

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