CN208857299U - The kit of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection - Google Patents

Abstract

The utility model discloses the kits of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection, including kit bottom, the bottom both ends at the kit bottom are bolted there are two non-slip mat, the top side at the kit bottom is bolted there are two hinge, and reagent box cover has been bolted in the side of two hinges.In the utility model, the circumferential inner wall of culture pedestal is provided with rubber retention bead, and bottom is provided with rubber pad, when carrying out culture medium placement, so that culture medium empties and keeps stable, and the temperature that temperature sensor measures is also the environment temperature emptied, the environment of culture medium is made to be more nearly demand of specialty in this way, it can also be improved the success rate of stem cell differentiation, the circumferential inner wall of reagent groove is pierced provided with silica gel hair down, its stabilization can be kept when placing reagent test tube, can be taken off power i.e. slightly upwards, texture is increased on the basis of the safety is improved.

Description

The kit of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection

Technical field

The utility model relates to field of biotechnology more particularly to the inductions of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection Kit.

Background technique

Umbilical cord mesenchymal stem cells, which refer to, is present in one of neonatal umbilical cord tissue versatile stem cell, it can break up At many kinds of histocytes, there is wide potential applicability in clinical practice, it can Successful amplification people using inactivation cord serum cultivating system Umbilical cord mesenchymal stem cells, the cell of culture have the fundamental characteristics of mescenchymal stem cell, for establish mesenchyma stem cell and Clinical application provides theoretical foundation.

Currently, the biotechnology research development in China be also it is more and more prosperous, for research also the seeking in effort of stem cell Method is looked for go to develop towards target, for Cord blood mesenchymal stem cells, it can be divided into different human tissue organ's cells And biological theory can replace the use of other organs of human body, and the direction of differentiation needs specified environment, so needing a kind of examination Agent box meets present biological study.

Summary of the invention

Purpose of the utility model is to solve disadvantages existing in the prior art, and the umbilical cord mesenchyma proposed is dry thin The kit of the induction of born of the same parents' Osteoblast Differentiation and detection.

To achieve the goals above, the utility model adopts the technical scheme that

The kit of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection, including kit bottom, the kit bottom Bottom both ends be bolted there are two non-slip mat, there are two the top side at the kit bottom is bolted Reagent box cover has been bolted in hinge, the side of two hinges, and a lateral roof of the reagent box cover passes through bolt It is connected with ultraviolet radiator, the first hole is offered among the side of the reagent box cover, and the first hole has been bolted Motor, circumferential inner wall one end of the fan shroud one has been bolted in fan shroud one, the side inner wall of the fan shroud one Web plate is bolted, the top one end at the kit bottom opens up fluted one, and groove one has been bolted Pedestal is cultivated, rubber pad has been bolted in the bottom interior wall of the culture pedestal, and the circumferential inner wall of the culture pedestal is logical It crosses and has been bolted rubber retention bead, the bottom of the culture pedestal has been bolted semiconductor chilling plate, and described half Heat-dissipating aluminium plate has been bolted in the bottom of conductor cooling piece, and the bottom end at the kit bottom offers the second hole, And second hole be bolted fan shroud two, heat dissipation has been bolted at the top of the circumferential inner wall of the fan shroud two Temperature sensor has been bolted in the circumferential inner wall of motor, the culture pedestal.

Preferably, the circumferential inner wall bottom of the fan shroud two has been bolted dedusting web plate, and fan shroud two It socket-connects at the top of outer circumferential walls with heat-dissipating aluminium plate.

Preferably, slide holder, and the interior bottom of slide holder have been bolted at the top of the kit bottom Portion is placed with slide.

Preferably, spring base has been bolted in the side inner wall of the slide holder, and one end of spring base is logical It crosses and has been bolted silicagel pad.

Preferably, fluted two are opened up at the top of the kit bottom, and reagent articles have been bolted in groove two Box.

Preferably, reagent groove is offered at the top of the reagent articles box, and the circumferential inner wall of reagent groove passes through spiral shell It tethers and is connected to silica gel hair down thorn.

Preferably, the side of the reagent box cover has been bolted controller, and controller by switch and motor, Semiconductor chilling plate, temperature sensor, radiating motor, ultraviolet radiator are connected.

The utility model has the following beneficial effects:

1, the kit among the reagent box cover of kit be provided with hole, and be equipped with motor, operator into It is run when row operation, it is close to bacterium is dusted and prevented around operating environment, and device is equipped with ultraviolet radiator, experiment Sterilization operation can be carried out before detection.

2, the circumferential inner wall of the culture pedestal of kit is provided with rubber retention bead and bottom is provided with rubber pad, When carrying out culture medium placement, so that culture medium empties and keeps stable, and the temperature that temperature sensor measures is also to empty Environment temperature, so that the environment of culture medium is more nearly demand of specialty, can also be improved stem cell differentiation success rate.

3, kit is improved in the design of reagent groove, is fallen in the circumferential inner wall of reagent groove provided with silica gel Burr can keep its stabilization when placing reagent test tube, and the design of hair down thorn can prevent from surprisingly spilling reagent test tube, It can be taken off power i.e. slightly upwards, texture increased on the basis of the safety is improved.

Detailed description of the invention

Fig. 1 be the utility model proposes umbilical cord mesenchymal stem cells Osteoblast Differentiation induction and detection kit vertical view Structural schematic diagram；

Fig. 2 be the utility model proposes the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiations and the kit of detection face Structural schematic diagram；

Fig. 3 be the utility model proposes umbilical cord mesenchymal stem cells Osteoblast Differentiation induction and detection kit part Structural schematic diagram.

In figure: 1 kit bottom, 2 culture pedestals, 3 rubber retention beads, 4 rubber pads, 5 reagent box covers, 6 hinges, 7 blowers Cover one, 8 motors, 9 web plates, 10 slides, 11 spring bases, 12 slide holders, 13 kits, 14 reagent grooves, 15 dedusting web plates, It is 16 fan shrouds two, 17 heat-dissipating aluminium plates, 18 semiconductor chilling plates, 19 temperature sensors, 20 radiating motors, 21 controllers, 22 ultraviolet Line lamp, 23 non-slip mats, 24 silica gel hair downs thorn.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.

Referring to Fig.1-3, the kit of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection, including kit bottom 1, examination The bottom both ends of agent cassette bottom 1 are bolted there are two non-slip mat 23, and the top side at kit bottom 1 is bolted There are two hinge 6, reagent box cover 5 has been bolted in the side of two hinges 6, and a lateral roof of reagent box cover 5 passes through spiral shell It tethers and is connected to ultraviolet radiator 22, the first hole is offered among the side of reagent box cover 5, and the first hole has been bolted Motor 8 has been bolted in fan shroud 1, the side inner wall of fan shroud 1, and circumferential inner wall one end of fan shroud 1 passes through It is bolted web plate 9, the top one end at kit bottom 1 opens up fluted one, and culture medium has been bolted in groove one Rubber pad 4 has been bolted in seat 2, the bottom interior wall of culture pedestal 2, and the circumferential inner wall of culture pedestal 2 is bolted There is rubber retention bead 3, semiconductor chilling plate 18 has been bolted in the bottom of culture pedestal 2, semiconductor chilling plate 18 Heat-dissipating aluminium plate 17 has been bolted in bottom, and the bottom end at kit bottom 1 offers the second hole, and the second hole passes through It has been bolted fan shroud 2 16, radiating motor 20, culture medium has been bolted at the top of the circumferential inner wall of fan shroud 2 16 Temperature sensor 19 has been bolted in the circumferential inner wall of seat 2.

In the utility model, dedusting web plate 15, and blower has been bolted in the circumferential inner wall bottom of fan shroud 2 16 It covers and socket-connects at the top of 2 16 outer circumferential walls with heat-dissipating aluminium plate 17, slide placement has been bolted in the top at kit bottom 1 Box 12, and the interior bottom of slide holder 12 is placed with slide 10, the side inner wall of slide holder 12 has been bolted Spring base 11, and one end of spring base 11 has been bolted silicagel pad, the top at kit bottom 1 open up fluted two, and Reagent articles box 13 has been bolted in groove two, and the top of reagent articles box 13 offers reagent groove 14, and reagent is recessed Silica gel hair down thorn 24 has been bolted in the circumferential inner wall of slot 14, and controller has been bolted in the side of reagent box cover 5 21, and controller 21 passes through switch and motor 8, semiconductor chilling plate 18, temperature sensor 19, radiating motor 20, ultraviolet radiator 22 are connected.

Working principle: before operator uses the kit, kit being opened, and the outlet of kit is accessed Power supply is ready for testing and detecting work, and the person of being operated first controls motor 8, radiating motor by the controller 21 of kit 20 operations, and can be automatically closed after controlling the work of ultraviolet radiator 22 two minutes of kit, operating environment is sterilized, is operated The required environment temperature of Cord blood mesenchymal stem cells Osteoblast Differentiation is entered into controller 21 by personnel by the data of profession, and And controller 21 controls semiconductor chilling plate 18 and works, and maintains the environment in cooperation 19 pairs of culture pedestals 2 of temperature sensor Required reagent is placed in the reagent groove 14 in the reagent articles box 13 of device, slide 10 is placed by temperature, operator It in slide holder 12, and is clipped, subsequent operator carries out the experiment and detection operation of profession.

The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model Within enclosing.

Claims (7)

1. the kit of the induction of umbilical cord mesenchymal stem cells Osteoblast Differentiation and detection, including kit bottom (1), which is characterized in that The bottom both ends of the kit bottom (1) are bolted there are two non-slip mat (23), the top of the kit bottom (1) Side is bolted there are two hinge (6), and reagent box cover (5) have been bolted in the side of two hinges (6), One lateral roof of the reagent box cover (5) has been bolted ultraviolet radiator (22), among the side of the reagent box cover (5) The first hole is offered, and fan shroud one (7), the side inner wall of the fan shroud one (7) has been bolted in the first hole It has been bolted motor (8), circumferential inner wall one end of the fan shroud one (7) has been bolted web plate (9), described The top one end at kit bottom (1) opens up fluted one, and culture pedestal (2), the culture has been bolted in groove one The bottom interior wall of pedestal (2) has been bolted rubber pad (4), and the circumferential inner wall of culture pedestal (2) is connected by bolt It is connected to rubber retention bead (3), the bottom of culture pedestal (2) has been bolted semiconductor chilling plate (18), described The bottom of semiconductor chilling plate (18) has been bolted heat-dissipating aluminium plate (17), and the bottom end of the kit bottom (1) is opened Equipped with the second hole, and fan shroud two (16), the circumferential inner wall of the fan shroud two (16) has been bolted in the second hole Top has been bolted radiating motor (20), and temperature biography has been bolted in the circumferential inner wall of culture pedestal (2) Sensor (19).

2. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 1 and detection, feature exist In, the circumferential inner wall bottom of the fan shroud two (16) has been bolted dedusting web plate (15), and fan shroud two (16) It socket-connects at the top of outer circumferential walls with heat-dissipating aluminium plate (17).

3. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 1 and detection, feature exist In being bolted slide holder (12) at the top of the kit bottom (1), and the interior bottom of slide holder (12) It is placed with slide (10).

4. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 3 and detection, feature exist In the side inner wall of the slide holder (12) has been bolted spring base (11), and one end of spring base (11) is logical It crosses and has been bolted silicagel pad.

5. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 1 and detection, feature exist In opening up fluted two at the top of the kit bottom (1), and reagent articles box (13) has been bolted in groove two.

6. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 5 and detection, feature exist In being offered reagent groove (14) at the top of the reagent articles box (13), and the circumferential inner wall of reagent groove (14) passes through spiral shell It tethers and is connected to silica gel hair down thorn (24).

7. the kit of umbilical cord mesenchymal stem cells Osteoblast Differentiation induction according to claim 1 and detection, feature exist In the side of the reagent box cover (5) has been bolted controller (21), and controller (21) passes through switch and motor (8), semiconductor chilling plate (18), temperature sensor (19), radiating motor (20), ultraviolet radiator (22) are connected.