CN208795642U - A kind of microflow control technique original position NH 3 device and its flow cell - Google Patents

Abstract

The utility model relates to monitoring water quality on line field more particularly to a kind of microflow control technique original position NH 3 devices and its flow cell.A kind of flow cell, including the main body being made of the opaque organic glass of black；The body interior is equipped with runner, and runner includes input section, detection section and deferent segment；The both ends of the input section, detection section and deferent segment are provided in main body wall, and the terminal part of input section and the top portion of deferent segment are connected with the both ends of detection section respectively, and the main body wall for being connected to position is closed by transparent glass sheet；The linear setting of the detection section, the angle that input section, deferent segment and detection section are constituted is acute angle.The flow cell can be effectively prevented detection cell primary path end positions and be detained bubble, influences to test signal, and strengthen the cleaning effect of flow cell, reduces the memory effect of test.

Description

A kind of microflow control technique original position NH 3 device and its flow cell

Technical field

The utility model relates to monitoring water quality on line field more particularly to a kind of microflow control technique original position NH 3 devices And its flow cell.

Background technique

Ambient water quality monitors the major tasks for having become Environmental Protection in China field on-line.Ammonia nitrogen is as in conventional water quality monitoring One of the important indicator of nutrient salts, be even more widely used.But equipment is with big cabinet sequence sample introduction at this stage Based on monitoring, reagent consumption is at 1-2mL/ times.Reagent consumption is larger, and O&M cost is high, and to operation station condition It is more demanding, it is difficult to for such as sea, lake surface, the complex working conditions such as river shoal.And it is supervised online in existing small portable water quality In measurement equipment, by taking Water quality ammonia nitrogen as an example.Existing electrochemical ion selects electrode equipment, kit class detector.But it is different Degree weakness.Wherein electrochemical apparatus anti-interference ability and parallel stability are poor, and data confidence is not high.Kit class testing Means precision is insufficient, and very big to visually observe colour developing generation error.So needs are more accurate, stablize, the water quality of miniaturization Online monitoring instruments.

The concept of liquid core waveguide is in the proposition of eighties of last century the seventies, the borosilicate glass or folding for being 1.51 by refractive index The quartz glass capillary that rate is 1.45 is penetrated, the liquid of high refractive index of the inside full of refractive index greater than 1.51 or 1.45 is constituted, Incident light can be in waveguide internal communication, since waveguide cores are liquid, so referred to as liquid core waveguide.Due to that can not find at that time Refractive index is less than the material of water (RI~1.33), therefore liquid core waveguide can not be applied to the chemical analysis detection of aqueous solution.1989 Year, DuPont has invented a kind of amorphous polymer of tetrafluoroethene --- and Teflon AF has pushed instrument analysis and light The development of the technical fields such as spectrum analysis.There are Teflon AF-1600 and Teflon AF-2400 two types, refractive index point at present It Wei not 1.31 and 1.29, the respectively less than refractive index of water.The liquid core waveguide manufactured by Teflon AF, after being full of aqueous solution, incident light It can be totally reflected in the interface of Teflon AF and water, light beam is strapped in waveguide and is propagated.Teflon AF liquid core waveguide For not only can be used as optical path pool, but also can be used as flow path sample cell, reducing costs, just in instrument analysis and spectrum analysis Realize promptly low-loss, small volume, small size long light path flow cell；Along with the liquid core waveguide manufactured by Teflon AF Can it is tortuous, there is the characteristic outstanding such as gas permeability so that this liquid core waveguide is rapidly in long light path absorption analysis, fluorescence point The numerous areas such as analysis, Raman spectrum analysis and gas sensor are widely used.

Currently, there are two types of models for common Teflon AF liquid core waveguide, one is the hairs manufactured by Teflon AF2400 Tubule, referred to as a type liquid core waveguide, another kind are the quartz capillary of outer surface plating Teflon AF2400 covering, referred to as two types Liquid core waveguide.Do not having relative to the flow cell in traditional instrument analysis using Teflon AF liquid core waveguide as flow cell Have in the case where increasing sample volume and light energy loss, effectively increases effective light path, and then can with the order of magnitude improve Detection sensitivity.

Teflon AF liquid core waveguide pipe has tortuous property, is very beneficial for the Miniaturization Design of instrument.Simultaneously as Teflon AF has gas permeability structure, hydrophobic characteristic, can be used as gas-permeable membrane use, has numerous scholars Novel fibre optic chemical sensor is devised for measuring the gas in aqueous solution and atmosphere.But just because of Teflon AF High sensitivity, gas permeability and the hydrophobic characteristic that liquid core waveguide possesses cause a type Teflon AF liquid core waveguide than two Type Teflon AF liquid core waveguide is easier to adhere to micro-bubble on inner wall, and then is easier to cause disturbing for optical signal when measurement It is dynamic；And Teflon AF liquid core waveguide flow cell is very " sensitivity ", and it is careless slightly in slight pollution or use, it will be tight Ghost image rings measurement result.

Summary of the invention

To solve the above-mentioned problems, the first of the utility model is designed to provide a kind of flow cell, which can be with It effectively prevent detection cell primary path end positions to be detained bubble, influences to test signal, and strengthen the cleaning effect of flow cell, Reduce the memory effect of test；The second of the utility model is designed to provide a kind of microflow control technique original position NH 3 dress It sets, which has above-mentioned flow cell.

In order to achieve the above purpose, the utility model uses technical solution below:

A kind of flow cell, it is characterised in that: including the main body being made of the opaque organic glass of black；The body interior Equipped with runner, runner includes input section, detection section and deferent segment；It opens at the both ends of the input section, detection section and deferent segment It is located in main body wall, the terminal part of input section and the top portion of deferent segment are connected with the both ends of detection section respectively, and even The main body wall at logical position is closed by transparent glass sheet；The linear setting of the detection section, input section, deferent segment and detection The angle that section is constituted is acute angle.

Preferably, the pipe diameter of the runner is 0.5mm.

Preferably, the main body is cuboid, the both ends for detecting section are provided with respectively at two lateral walls center, defeated The terminal part of the top portion and deferent segment that enter section is provided at the center of other two opposite side walls.

Preferably, the input section of the runner, detection section and deferent segment are in the same plane.

A kind of microflow control technique original position NH 3 device, including pedestal, and setting flow cell on the base, water inlet Pipeline, outlet pipeline, LED lamp source and photoelectric detection component；It is characterized by: a kind of flow cell circulation as described above Pond, the inlet pipeline are connected in the top portion of input section, and outlet pipeline is connected to the terminal part of deferent segment；The LED light Source and photoelectric detection component correspond respectively to the both ends of detection section, and the projecting direction of LED lamp source is directed toward light along detection section Electro-detection component.It is related to a kind of NH 3 device in the technical solution, the inlet pipeline in the NH 3 device introduces inspection Liquid is surveyed, cocurrent enters in the runner of flow cell, and outlet pipeline is for drawing waste liquid；The light that LED lamp source issues can pass through runner Detection section, and absorbance is calculated after receiving via photoelectric detection component, must be discharged in ammonia nitrogen concentration.

Preferably, being additionally provided with two pipe fitting fixing seats on the pedestal of the flow cell surrounding and two optical fiber are fixed Seat, water inlet pipe and water outlet pipe are fixed by pipe fitting fixing seat, and LED lamp source and photoelectric detection component pass through fiber fixed seat It is fixed.

Preferably, further including upper cover, the upper cover is covered on flow cell, pipe fitting fixing seat and fiber fixed seat Side.

By adopting the above technical scheme, which is related to a kind of flow cell to the utility model, which includes by black The main body that the opaque organic glass of color is constituted, black acrylic (PMMA) good shading effect.Body interior is equipped with runner, detection When the light that issues of optical fiber can only pass through runner section light transmission, determine rate according to lambert Bill, the length of light path can meet in high precision Ammonia nitrogen concentration detects demand.The runner includes input section, detection section and deferent segment, and detects the linear setting of section, input The angle that section, deferent segment and detection section are constituted is acute angle；Using the circulation sample introduction design of unique Z-type, compared to conventional orthogonal Runner can be effectively prevented detection cell primary path end positions and be detained bubble, influence to test signal, and strengthen flow cell Cleaning effect reduces the memory effect of test.And the transparent glass sheet in main body wall can guarantee light transmission rate and high stable Property.Quartz material is stablized, indeformable discoloration, ensure that the feelings of leakage or failure can not occur for a long time with stable operation for flow cell Condition.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of ammonia nitrogen on-line computing model in situ.

Fig. 2 is the structural schematic diagram of micro-fluidic chip.

Fig. 3 is the structural schematic diagram one (no upper cover) of NH 3 device.

Fig. 4 is the structural schematic diagram two (having upper cover) of NH 3 device.

Fig. 5 is the structural schematic diagram of flow cell.

Specific embodiment

With reference to the accompanying drawing, the preferred embodiment of the utility model is described in further detail.

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.

In the description of the present invention, it should be understood that term " on ", "lower", "front", "rear", "left", "right", The orientation or positional relationship of the instructions such as "top", "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific Orientation, be constructed and operated in a specific orientation；Therefore it should not be understood as limiting the present invention.Also, term " on Trip " and " downstream " are on the basis of the flow direction of fluid in entire line monitor.

One kind as shown in Fig. 1~5 be based on microflow control technique original position ammonia nitrogen on-line computing model, including transfusion system 1 and It is connected to the micro-fluidic chip 2 in 1 downstream of transfusion system, and is connected to the NH 3 device 3 in 2 downstream of micro-fluidic chip.It should Technical solution is related to one kind based on microflow control technique original position ammonia nitrogen on-line computing model, and the on-line computing model is with 2 skill of micro-fluidic chip Art is core, constructs a set of micro-full analytical system and is automatically monitored on-line to Water quality ammonia nitrogen.With reach miniaturization, it is miniature Change, facilitation, micro-sampling and the purpose quickly detected.Specifically, below for defeated in ammonia nitrogen on-line computing model in situ Liquid system 1, micro-fluidic chip 2 and NH 3 device 3 are described in detail.

Transfusion system

The transfusion system 1 includes clear water pipeline 11, standard liquid pipeline 12, water sample pipeline 13, the first reagent cartridge 14 and the Two reagent cartridges 15.Also, the clear water pipeline 11, standard liquid pipeline 12, water sample pipeline 13, the first reagent cartridge 14 and the second examination Agent pipeline 15 is both connected on gas flow control device 16.As shown in fig. 1, clear water pipeline 11, standard liquid pipeline 12, water sample pipe Road 13, the first reagent cartridge 14 and the second reagent cartridge 15 respectively by fresh water container 111, standard liquid container 121, water sample container 131, First reagent container 141 and the second reagent container 151 are drawn, after gas flow control device 16 introduces air into said vesse, Corresponding Pipeline transport liquid is driven by atmospheric pressure.The program pushes water sample sample introduction by Pneumatic constant pressure, and realization is micro, even It is continuous, stablize, few the advantages that pumping few valve.Use a pneumatic supply, connect four groups of partial pressure valves and switch valve, directly with rear end air pressure Reagent, water sample ejected wash water of driving etc. are connected, and reduce a large amount of triple valve, multi-way valve structure demand.

Micro-fluidic chip 2

The micro-fluidic chip 2 includes glass-chip ontology 21.The inside of the glass-chip ontology 21 is equipped with chip stream Road.The chip runner includes along the sequentially connected liquid input section in fluid conveying direction, mixing section and liquid section 28 out.The liquid input section Including main feed liquor tube side 22, and the clear water tube side 23, standard liquid tube side 24, water sample tube side 25 and the examination that are communicated with main feed liquor tube side 22 Agent tube side.The joint of the standard liquid tube side 24, water sample tube side 25 and reagent tube side and main feed liquor tube side 22 is along fluid conveying side To setting gradually.The joint of the clear water tube side 23 and main feed liquor tube side 22 is not at standard liquid tube side 24 and main feed liquor tube side 22 Joint downstream.The reagent tube side includes the first reagent tube side 26 and the second reagent tube side 29.First reagent tube side 26, Two reagent tube sides 29 are intersected in same point with main feed liquor tube side 22.Wave tube side 27 is equipped in the mixing section, further Specifically in embodiment, mixing section includes a plurality of wave tube side 27 for being arranged side by side and being sequentially connected.Above-mentioned micro-fluidic chip 2 The aperture of chip runner be 0.5mm, including liquid input section, mixing section and liquid section 28 out.The transfusion system 1 is including respectively and clearly The clear water pipeline that water pipe journey 23, standard liquid tube side 24, water sample tube side 25, the first reagent tube side 26 are connected with the second reagent tube side 29 11, standard liquid pipeline 12, water sample pipeline 13, the first reagent cartridge 14 and the second reagent cartridge 15.

In the micro-fluidic chip 2, clear water tube side 23 is placed in the port of entire flow path most started, make test beginning and The cleaning process of end state is easily and thoroughly.Standard liquid tube side 24 is placed in front of water sample tube side 25, and makes standard specimen by cleaning process With water sample physical isolation, its cross contamination from each other is prevented.29 import position of first reagent tube side 26 and the second reagent tube side It is overlapped, purpose makes the sample introduction synchronous with reagent two of reagent one, synchronized mixes.The subsequent mixing section for having installed relatively long distance additional, mixing section For wave tube side 27, main function is to make to be sufficiently mixed between reagent one, reagent two and water sample three, is entered after mixing subsequent It is detected in micro- circulation detecting pool.The utility model by adopting the above technical scheme, with micro-fluidic chip 2 have compact structure and It can portable carrying feature.Simultaneously as reagent consumption is small using microfluidic technology, and about 100 microlitres of amount of reagent, conventional instrument Device consumption is 1mL, about 1/10th or so.Reagent consumption is small, and cost reduces, and pollution reduces.And chip can the several seconds extremely Measurement, separation or other more complicated operations are automatically performed in tens of seconds times.Analysis and separating rate are often higher than macroscopic analysis One or two order of magnitude of method.By taking ammonia nitrogen is tested as an example, the conventional on-line monitoring ammonia nitrogen instrument test time usually requires 20min, And detection can be completed in In Microfluidic Analytical Systems within 2 minutes.

NH 3 device 3

The NH 3 device 3 include pedestal 31, and be arranged on pedestal 31 flow cell 32, inlet pipeline 33, Outlet pipeline 34, LED lamp source 35 and photoelectric detection component 36.

Wherein, the flow cell 32 includes the main body 321 being made of the opaque organic glass of black.In the main body 321 Portion is equipped with runner, and runner includes input section 322, detection section 323 and deferent segment 324.The input section 322, detection section 323 and defeated The both ends of section 324 are provided on 321 side wall of main body out, the terminal part of input section 322 and the top portion difference of deferent segment 324 It is connected with the both ends of detection section 323, and 321 side wall of main body for being connected to position is closed by transparent glass sheet 325.The inspection The linear setting of section 323 is surveyed, the angle that input section 322, deferent segment 324 and detection section 323 are constituted is acute angle；Wherein one In kind specific embodiment, the main body 321 of the flow cell 32 is the cuboid of 10mm*10mm*20mm, the input section of runner 322, it detects section 323 and deferent segment 324 is in the same plane.The both ends of detection section 323 are provided with two lateral walls respectively At center, the top portion of input section 322 and the terminal part of deferent segment 324 are provided at the center of other two opposite side walls；Detection The length of section 323 is 10mm, and the runner internal diameter including input section 322, detection section 323 and deferent segment 324 is 0.5mm.It is above-mentioned Flow cell 32 in scheme includes the main body 321 being made of the opaque organic glass of black, black acrylic (PMMA) shading Effect is good.Runner is equipped with inside main body 321, the light that optical fiber issues when detection can only pass through runner section light transmission, according to bright Bobi You determine rate, and the length of light path can meet high-precision ammonia nitrogen concentration detection demand.The runner includes input section 322, detection section 323 and deferent segment 324, and the linear setting of section 323 is detected, what input section 322, deferent segment 324 and detection section 323 were constituted Angle is acute angle.Using the circulation sample introduction design of unique Z-type, compared to conventional orthogonal runner, detection cell master can be effectively prevented Access end positions are detained bubble, influence to test signal, and strengthen the cleaning effect of flow cell 32, reduce the memory of test Effect.And the transparent glass sheet 325 on 321 side wall of main body can guarantee light transmission rate and high stability.Quartz material is stablized, no Deformation discoloration, ensure that flow cell 32 can with stable operation for a long time there is a situation where leakage or failure.

Two pipe fitting fixing seats 37 and two fiber fixed seats 38 are additionally provided on the pedestal 31 of 32 surrounding of flow cell, Inlet pipeline 33 and outlet pipeline 34 are fixed by pipe fitting fixing seat 37, and LED lamp source 35 and photoelectric detection component 36 pass through light Fine fixing seat 38 is fixed.The NH 3 device 3 further includes upper cover 39, and the upper cover 39 is covered on flow cell 32, pipe fitting 38 top of fixing seat 37 and fiber fixed seat.Wherein, the beginning of the inlet pipeline 33 is connected to the output end of micro-fluidic chip 2 On, end is connected in 322 top portion of input section of flow cell 32, and outlet pipeline 34 is connected to the terminal part of deferent segment 324.Institute It states LED lamp source 35 and photoelectric detection component 36 corresponds respectively to the both ends of detection section 323, and the projection side of LED lamp source 35 Photoelectric detection component 36 is directed toward to along detection section 323.It is related to a kind of NH 3 device 3, the NH 3 in the technical solution Inlet pipeline 33 in device 3 introduces detection liquid, and cocurrent enters in the runner of flow cell 32, and outlet pipeline 34 is useless for drawing Liquid.The light that LED lamp source 35 issues can be calculated by the detection section 323 of runner, and after receiving via photoelectric detection component 36 Absorbance, must be discharged in ammonia nitrogen concentration.

The analysis flow path workflow of ammonia nitrogen on-line computing model in situ

1) water sample is replaced: water sample sample introduction, remaining mouthful of position is closed, and flow path control replaces 1min in 200 μ L/min, water sample.Make Water sample is full of microchip, and detects blank signal in micro- flow cell 32 in real-time online at this time.

2) color developing detection: water sample sample introduction, one sample introduction of reagent, two sample introduction of reagent, remaining mouthful of position are closed；Every flow path of flow path is equal Control is in 50 μ L/min；After replacing 20s, the color signal in the micro- flow cell 32 of real-time in-line testing.By color signal value and Absorbance is calculated in blank signal value, must be discharged in ammonia nitrogen concentration.

3) it cleans: after detecting color signal, opening ejected wash water implication pump, other mouthful of position is closed；Ejected wash water sample introduction is pushed, Ejected wash water flow path is controlled in 200 μ L/min；Ejected wash water cleans entire chip and further microfluidic leads to pond 32, and rinsing 1min can be into Sample.

Above-mentioned steps 2) in colour developing tested using conventional salicylic acid method, agent prescription is following (to prepare mark with 1L amount It is quasi-):

Reagent one

According to 130g/L, (weight readings are accurate to ± 2g) weighs sodium salicylate and trisodium citrate respectively, pours into 1L's In beaker, the water of beaker ull-scale value about 80% is added, is then stirred with glass bar, until being completely dissolved.It adds (weight is accurate to ± 0.02g to the sodium nitroprussiate of 1.0g/L, since sodium nitroprussiate is hard solid, when weighing, should control the big of fragment It is small；) dissolution when, stirred on one side using glass bar in beaker center, on one side slightly smash sodium nitroprussiate solid to pieces, until beaker bottom is seen Less than the tiny particles content and then addition distilled water that can be glistened to ull-scale value, after finally being stirred three minutes with glass bar again, It stands, for use.

Reagent two

32g/L is weighed, the sodium hydroxide of (weight degree is accurate to ± 0.2g) is poured slowly into the beaker of 1L, is slowly added The distilled water for entering beaker ull-scale value 50%, is constantly stirred with glass bar, until sodium hydroxide be completely dissolved (sodium hydroxide if any Caking phenomenon can slightly be smashed to pieces with glass bar, accelerate dissolution).After dissolution, solution is waited to be cooled to room temperature, then added again 2.0g/L sodium dichloro cyanurate (weight readings essence is added in the distilled water for entering about 30% beaker ull-scale value while stirring Really to ± 0.1g).Until completely dissolved, then plus distilled water to beaker ull-scale value at.Stirring three minutes is stood, for use.

Standard solution

(returned using pan paper by the way of claiming, actual weight is the difference of front and back weight twice) using weight reduction, weigh in (reading precisely to ± 0.005g), pours into ammonium chloride (NH4CL, standard items) 3.8190g/L of 100~105 DEG C of dryings 2 hours In 1000mL volumetric flask, after the water that addition accounts for about 80% volume of volumetric flask is completely dissolved, it is settled to graduation mark with water, is shaken up, it is quiet It sets, for use.

The entire testing process of above-mentioned original position ammonia nitrogen on-line computing model is less than 4min.Better than conventional method for testing ammonia nitrogen The testing time of 20min is 100 microlitres to one reagent of reagent, two consumption, much smaller than conventional method for testing ammonia nitrogen quantitative loop into 1000 microlitres of each sample volumes of sample；Ejected wash water consumption is that 2mL is each, again smaller than conventional ammonia nitrogen ejected wash water consumption.

The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model Within enclosing.

Claims (7)

1. a kind of flow cell, it is characterised in that: including the main body being made of the opaque organic glass of black；The body interior is set There is runner, runner includes input section, detection section and deferent segment；The both ends of the input section, detection section and deferent segment open up In main body wall, the terminal part of input section and the top portion of deferent segment are connected with the both ends of detection section respectively, and are connected to The main body wall at position is closed by transparent glass sheet；The linear setting of the detection section, input section, deferent segment and detection section The angle constituted is acute angle.

2. a kind of flow cell according to claim 1, it is characterised in that: the pipe diameter of the runner is 0.5mm.

3. a kind of flow cell according to claim 1, it is characterised in that: the main body is cuboid, detects the both ends of section Portion is provided with respectively at two lateral walls center, and the top portion of input section and the terminal part of deferent segment are provided with other two opposite sides At the center of wall.

4. a kind of flow cell according to claim 3, it is characterised in that: input section, detection section and the output of the runner Section is in the same plane.

5. a kind of microflow control technique original position NH 3 device, including pedestal, and flow cell, the water inlet pipe of setting on the base Road, outlet pipeline, LED lamp source and photoelectric detection component；It is characterized by: any one of described flow cell such as Claims 1 to 4 A kind of flow cell, the inlet pipeline are connected in the top portion of input section, and outlet pipeline is connected to the end of deferent segment End；The LED lamp source and photoelectric detection component correspond respectively to the both ends of detection section, and the projecting direction of LED lamp source Photoelectric detection component is directed toward along detection section.

6. a kind of microflow control technique original position NH 3 device according to claim 5, it is characterised in that: the flow cell Two pipe fitting fixing seats and two fiber fixed seats are additionally provided on the pedestal of surrounding, water inlet pipe and water outlet pipe is connect by pipe Head fixing seat is fixed, and LED lamp source and photoelectric detection component are fixed by fiber fixed seat.

7. a kind of microflow control technique original position NH 3 device according to claim 6, it is characterised in that: on further including Lid, the upper cover are covered on above flow cell, pipe fitting fixing seat and fiber fixed seat.