CN208791660U - A kind of deep-processed food DNA extraction kit - Google Patents
A kind of deep-processed food DNA extraction kit Download PDFInfo
- Publication number
- CN208791660U CN208791660U CN201821399199.XU CN201821399199U CN208791660U CN 208791660 U CN208791660 U CN 208791660U CN 201821399199 U CN201821399199 U CN 201821399199U CN 208791660 U CN208791660 U CN 208791660U
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- partition
- back box
- deep
- processed food
- dna extraction
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
The utility model relates to a kind of deep-processed food DNA extraction kits, including back box, it is placed with the first partition that back box is divided into two parts in the back box, first partition two sides outer wall is equipped with groove, and the groove is equipped with perpendicular to first partition and the second partition that is adapted to back box；Back box is divided into four reagent units by the first partition and second partition；The first partition and the second partition are hollow clapboard；The container hole for placing centrifuge tube is equipped in the first partition and the second partition.The utility model provides a kind of deep-processed food DNA extraction kit, improves DNA recovery rate, best in quality, extraction process is simple and quick, and experimental efficiency is high, ensure that the accuracy of test data, improves work efficiency.
Description
Technical field
The utility model relates to kit fields, and in particular to a kind of deep-processed food DNA extraction kit.
Background technique
Due to food composition complexity, added except the food such as salt, sugar, oil, pigment exceptionally, are also contained containing plurality of raw materials group
Agent, in addition, the techniques such as decocting, frying, boiling, baking and also the DNA in raw material can be made to will receive different degrees of damage in process.Cause
This, the difficulty more opposite than extracting DNA from raw material from extraction DNA in processed food.
Since DNA content is very low in deep-processed food, purity is low, and recovery rate is low to be not easy to obtain, and experimental period is long, therefore
One kind being efficiently specially adapted for deep-processed food DNA extraction kit and requires study.
Utility model content
The utility model provides a kind of deep-processed food DNA extraction kit, improves DNA recovery rate, best in quality, mentions
Take process simple and quick, experimental efficiency is high, ensure that the accuracy of test data, improves work efficiency.
The technical solution adopted in the utility model is as follows:
A kind of deep-processed food DNA extraction kit, including back box are placed in the back box back box being divided into two
Partial first partition, first partition two sides outer wall be equipped with groove, the groove be equipped with perpendicular to first partition and with
The second partition of back box adaptation；Back box is divided into four reagent units by the first partition and second partition；Described first every
Plate and the second partition are hollow clapboard；The appearance for placing centrifuge tube is equipped in the first partition and the second partition
Device hole.
The working principle and process of the technical program are as follows:
Firstly, back box is horizontally arranged, first partition is placed in back box, first partition divides equally the space in back box
For two parts, first partition is divided into two parts by the groove that the centre of the first partition is equipped with, groove；Described
Two partitions are adapted with groove, and second partition is mutually fixed by groove with first partition；And then first partition and second partition
It is in cross in back box, back box is divided into four parts, for placing different reagent bottles, such as buffer GMO1
(Buffer GMO1), buffer GMO2 (Buffer GMO2) 20, Proteinase K, elution buffer TE (Buffer TE)；
The container hole that the partition is equipped with, convenient for temporarily storing the centrifuge tube for being placed with experiment liquid in experimentation.The groove
Design is placed in position in back box and is not susceptible to deviate so that first partition is fixed to each other with second partition, convenient to use.
Further, the back box bottom is connected with pumping case component, and the pumping case component includes pumping case case and right
Erect the tool pumping case being placed in pumping case case, experiment pumping case.Tool pumping case is taken out for placing centrifuge tube needed for experiment, experiment
The tool and measurer for reagent to be added dropwise are placed in box.
Further, the pumping case box outer wall is not provided with tool pumping case and the opposite side of experiment pumping case is fixedly connected with hand
Support ring.The kit is monoblock type in use, can be split by holding ring mobile reagent box compared to common
The kit of formula, the kit, convenient for moving integrally, use is more convenient.
Further, it is arranged with the cover for covering back box and pumping case component at the top of the back box, the cover outer wall is equipped with
The through-hole being adapted to hand-held ring, the through hole are connected with the shadoof being adapted to cover.Cover, back box, pumping case case are mutual
It is flexibly connected, holds ring and pass through through-hole, shadoof hook holds hand-held ring, and then moves integrally entire kit, is easy to use.
Further, the first partition and the height of the second partition are greater than the back box height.First partition and
Second partition is above the height of back box, convenient for reagent bottle of taking, can be seen clearly that the label of reagent bottle, prevents from taking examination by mistake
Agent influences the progress of subsequent experimental, reduces experiment accuracy.
Further, the width of the groove, the thickness of the first partition, the second partition thickness be consistent
's.The width and second partition consistency of thickness of groove are placed in bottom convenient for second partition is fixed on first partition two sides jointly
In box, it is not easy to misplace in use process.
Further, the first partition is oblique from groove to back box edge.Experimenter is using kit
Centrifuge tube of frequently taking is needed in the process, so first partition is oblique, convenient for taking.The container hole of first partition is for putting
The centrifuge tube in use is set, the container of second partition is for placing not used centrifuge tube.
Further, the container hole there are six same aperture is all provided on the first partition and the second partition.
Further, slot tag is equipped at container hole on the first partition and the second partition.Deep processing food
Product DNA extracts experimental procedure and generally requires three centrifuge tubes, the corresponding container hole of a slot tag, by edge upper in first partition
Slot tag near back box edge to the container hole of groove direction is labeled as the first container hole, second container hole, third container hole.
The first container hole, second container hole, third container hole, which are followed successively by experiment, places different centrifuge tubes according to experimental procedure；The design
The reasonability being laid out in kit is further improved, the parking space in kit is taken full advantage of；It can be put in slot tag
Label label are set, so that the centrifuge tube stored on second partition more easily discriminates, and guarantees the record accuracy of related data, drop
Low experiment human error possibility.
In the technical program, related component, material is acrylic material；In addition to acrylic, it can also use
Other meet experiment condition and have the material of equivalent intensity.
Reagent bottle, centrifuge tube etc. in available reagent box is creatively rationally put together at one by the technical program
In kit, most of step carries out directly in kit in experimentation.Kit spells composition by each original part
The kit of one monoblock type, reagent bottle, centrifuge tube needed for experiment etc. are all placed in kit, can be straight in experimentation
Mobile entire kit is connect near centrifuge, improves conventional efficient.Layout designs in the kit are reasonable, make full use of
Conventional efficient is improved convenient for reagent bottle and the centrifuge tube of taking in space in kit, reduces human error possibility, facilitates shifting
It takes, it disposably can multiple groups test.
In conclusion the beneficial effect of the utility model compared to the prior art is:
(1) kit is divided into the mode of assembly in the utility model, so that the kit division of labor is clear, improves operation
Efficiency；Prevent reagent influence each other or reagent bottle it is similar caused by experiment be added dropwise mistake caused by the failure of an experiment；
(2) back box is set up separately and is independent space and is used to place by the design of first partition and second partition in the utility model
For placing centrifuge tube, the kit meets examination simultaneously for different reagent bottles, first partition and second partition upper container hole
The storage of agent bottle and centrifuge tube, convenient for distinguishing the storage position of different reagents；Convenient for taking for reagent bottle and centrifuge tube, improve
Working efficiency；And the tool pumping case in pumping case component can store extra centrifuge tube, and experiment pumping case can place experiment institute
The tool needed, improves the utilization rate and globality of kit；
(3) container hole of label slot marker different experiments step is opposite with experimental procedure convenient for distinguishing the step in experiment
It answers, improves conventional efficient, it is ensured that experiment precision；Entire majority steps of testing can be completed on kit, simplify operator
The experimental arrangement of member improves conventional efficient；Monoblock type kit is easy to remove, and flexibility is high.
Detailed description of the invention
Structural schematic diagram when Fig. 1 is a kind of deep-processed food DNA extraction kit unused state in the utility model
Structural schematic diagram when Fig. 2 is a kind of deep-processed food DNA extraction kit use state in the utility model
Fig. 3 is the structural schematic diagram of pumping case component in the utility model
Structural schematic diagram when Fig. 4 is a kind of deep-processed food DNA extraction kit opening cover in the utility model
In the figure, it is marked as 1- back box, 2- first partition, 3- groove, 4- second partition, 5- reagent bottle unit, 6- container hole,
7- pumping case component, 8- pumping case case, 9- tool pumping case, 10- test pumping case, and 11- holds ring, 12- cover, 13- through-hole, 14- shadoof,
15- slot tag.
Specific embodiment
All features disclosed in this specification can be with any other than mutually exclusive feature and/or step
Mode combines.
In order to make those skilled in the art more fully understand the technical solution of the utility model, below with reference to Fig. 1-3 and
The utility model is described in further detail for specific embodiment.
Embodiment 1
A kind of deep-processed food DNA extraction kit, including back box 1 are placed in the back box 1 and are divided into back box 1
The first partition 2 of two parts, the 2 two sides outer wall of first partition are equipped with groove 3, are equipped at the groove 3 perpendicular to first
Partition 2 and the second partition 4 being adapted to back box 1；Back box 1 is divided into four reagent lists with second partition 4 by the first partition 2
Member；The first partition 2 and the second partition 4 are hollow clapboard；In the first partition 2 and the second partition 4
Equipped with the container hole 6 for placing centrifuge tube.
Embodiment 2
Based on embodiment 1,1 bottom of back box is connected with pumping case component 7, and the pumping case component 7 includes pumping case case
8 and opposition be set in pumping case case 8 tool pumping case 9, experiment pumping case 10.Tool pumping case 9 is for placing needed for experiment
Centrifuge tube, the interior tool and measurer placed for reagent to be added dropwise of experiment pumping case 10.
Embodiment 3
Based on embodiment 1,8 outer wall of pumping case case is not provided with tool pumping case 9 and the opposite side of experiment pumping case 10 is fixed
It is connected with hand-held ring 11.The kit is monoblock type in use, can be by holding 11 mobile reagent box of ring, phase
Compared with the kit of common split-type, the kit, convenient for moving integrally, use is more convenient.
Embodiment 4
Based on embodiment 1, the cover 12 for covering back box 1 and pumping case component 7, the cover are arranged at the top of the back box 1
12 outer walls are equipped with the through-hole 13 being adapted to hand-held ring 11, are connected with the shadoof 14 being adapted to cover 12 at the through-hole 13.
Cover 12, back box 1, pumping case case 8 are mutually flexibly connected, and are held ring 11 and are passed through through-hole 13,14 hook of shadoof holds hand-held ring 11, in turn
Entire kit is moved integrally, is easy to use.
Embodiment 5
Based on embodiment 1, the height of the first partition 2 and the second partition 4 is greater than 1 height of back box.First
Partition 2 and second partition 4 are above the height of back box 1, convenient for reagent bottle of taking, can be seen clearly that the label of reagent bottle, prevent
It only takes reagent by mistake, influences the progress of subsequent experimental, reduce experiment accuracy.
Embodiment 6
Based on embodiment 1, the width of the groove 3, the thickness of the first partition 2, the second partition 4 thickness be
It is consistent.The width and 4 consistency of thickness of second partition of groove 3, convenient for second partition 4 is fixed on 2 two sides of first partition, jointly
It is placed in back box 1, is not easy to misplace in use process.
Embodiment 7
Based on embodiment 1, the first partition 2 is oblique from groove 3 to 1 edge of back box.Experimenter is using examination
Centrifuge tube of frequently taking is needed during agent box, so first partition 2 is oblique, convenient for taking.The container of first partition 2
Hole 6 is used to place the centrifuge tube in use, and the container of second partition 4 is for placing not used centrifuge tube.
Embodiment 8
Based on embodiment 1, the container hole there are six same aperture is all provided in the first partition 2 and the second partition 4
6。
Embodiment 9
Based on embodiment 1, slot tag 15 is equipped in the first partition 2 and the second partition 4 at container hole 6.
Deep-processed food DNA extracts experimental procedure and generally requires three centrifuge tubes, the corresponding container hole 6 of a slot tag 15, by the
Slot tag 15 on one partition 2 near the container hole 6 at 1 edge of back box to 3 direction of groove holds labeled as the first container hole, second
Device hole, third container hole；The first container hole, second container hole, third container hole be followed successively by according to experimental procedure place it is different from
Heart pipe；The design further improves the reasonability being laid out in kit, takes full advantage of the parking space in kit；Label
Label label can be placed in slot 15, so that the centrifuge tube stored on second partition 4 more easily discriminates, and guarantee related data
Record accuracy reduces experiment human error possibility.
Embodiment 10
Based on embodiment 1, the reagent bottle unit 5 places buffer GMO1 (Buffer GMO1), buffer GMO2 respectively
(Buffer GMO2) 20, Proteinase K, elution buffer TE (Buffer TE), tool pumping case 9 is for placing experiment institute
The centrifuge tube needed, tool, such as dropper needed for experiment pumping case 10 is used to place experiment etc.；In first partition 2 and second partition 4
Centrifuge tube can also be placed with.Before use, mobile shadoof 14 takes out cover 12；It takes out after a centrifuge tube (1) sequentially adds and pulverize
Deep processing food, buffer GMO1 and Proteinase K, vortex oscillation and be incubated for；Then, buffer GMO2 is added, fills
Divide and mixes, vortex oscillation；Centrifuge tube (1) is put into the first container hole, is stored at room temperature；Secondly, by the centrifuge tube (1) after standing from
Supernatant in above-mentioned centrifuge tube is transferred in new centrifuge tube (2) by the heart；Isopropanol is added in centrifuge tube (2), it is sufficiently mixed
Even centrifugation falls to abandon supernatant to centrifuge tube (3), retains precipitating, centrifuge tube (3) is put into second container hole at this time after static；After
Continue and ethyl alcohol is added in centrifuge tube (2), supernatant is abandoned in vortex oscillation centrifugation, repeats that ethyl alcohol is added again, vortex oscillation centrifugation is abandoned
Supernatant；It finally uncaps inversion, centrifuge tube (2) is put into third container hole, is stored at room temperature, thoroughly dry remaining ethyl alcohol, so
Elution buffer TE is added afterwards, vortex oscillation finally obtains DNA solution.Not by the liquid in centrifuge tube (3) in above-mentioned steps
It abandons, because " isopropanol being added in centrifuge tube (2), mixes well centrifugation, falls to abandon supernatant after static to centrifuge tube (3), protects
Stay precipitating " the step of in easily the precipitating in centrifuge tube (2) is poured into centrifuge tube (3) with supernatant, so by centrifuge tube
(3) it is placed in second container hole, analyzes experimental error convenient for subsequent examination, even by centrifuge tube (3) repeated centrifugation pipe (2) again
Experiment, extract DNA again, improve experiment accuracy.It is most important in the design and experimental procedure in the kit containers hole 6
Three centrifuge tubes are corresponding, and entire experiment removes centrifugation step, and majority is completed in kit, improve experiment accuracy, experiment
Efficiency, and the recovery rate of deep-processed food DNA is higher.
The specific embodiment of the application above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
The limitation to the application protection scope therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, under the premise of not departing from technical scheme design, various modifications and improvements can be made, these belong to this
The protection scope of application.
Claims (9)
1. a kind of deep-processed food DNA extraction kit, it is characterised in that: including back box (1), vertically set in the back box (1)
There is the first partition (2) that back box (1) is divided into two parts, first partition (2) the two sides outer wall is equipped with longitudinal groove
(3), it is equipped at the groove (3) perpendicular to first partition (2) and the second partition (4) that is adapted to back box (1)；Described first every
Back box (1) is divided into four reagent bottle units (5) in crosswise with second partition (4) by plate (2)；The first partition (2) with
The second partition (4) is hollow clapboard；Placement centrifugation is equipped on the first partition (2) and the second partition (4)
The container hole (6) of pipe.
2. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: back box (1) bottom
Portion is connected with pumping case component (7), and the pumping case component (7) includes that pumping case case (8) and opposition are set to pumping case case (8)
Interior tool pumping case (9), experiment pumping case (10).
3. a kind of deep-processed food DNA extraction kit according to claim 2, it is characterised in that: the pumping case case (8)
Outer wall is not provided with tool pumping case (9) and the opposite side of experiment pumping case (10) is fixedly connected with hand-held ring (11).
4. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: back box (1) top
Portion is arranged with the cover (12) for covering back box (1) and pumping case component (7), and cover (12) outer wall is equipped with suitable with hand-held ring (11)
The through-hole (13) matched is connected with the shadoof (14) being adapted to cover (12) at the through-hole (13).
5. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: the first partition
(2) and the height of the second partition (4) is greater than the back box (1) height.
6. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: the groove (3)
Width, the thickness of the first partition (2), the second partition (4) thickness be consistent.
7. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: the first partition
(2) oblique from groove (3) to back box (1) edge.
8. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: the first partition
(2) and on the second partition (4) it is all provided with the container hole (6) there are six same aperture.
9. a kind of deep-processed food DNA extraction kit according to claim 1, it is characterised in that: the first partition
(2) and on the second partition (4) slot tag (15) are equipped at container hole (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821399199.XU CN208791660U (en) | 2018-08-29 | 2018-08-29 | A kind of deep-processed food DNA extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821399199.XU CN208791660U (en) | 2018-08-29 | 2018-08-29 | A kind of deep-processed food DNA extraction kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208791660U true CN208791660U (en) | 2019-04-26 |
Family
ID=66207459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821399199.XU Expired - Fee Related CN208791660U (en) | 2018-08-29 | 2018-08-29 | A kind of deep-processed food DNA extraction kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208791660U (en) |
-
2018
- 2018-08-29 CN CN201821399199.XU patent/CN208791660U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190426 Termination date: 20200829 |