CN208786436U - It is a kind of using chromatographic sheet as the micro-fluidic chip of substrate - Google Patents
It is a kind of using chromatographic sheet as the micro-fluidic chip of substrate Download PDFInfo
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- CN208786436U CN208786436U CN201821199653.7U CN201821199653U CN208786436U CN 208786436 U CN208786436 U CN 208786436U CN 201821199653 U CN201821199653 U CN 201821199653U CN 208786436 U CN208786436 U CN 208786436U
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Abstract
The utility model discloses a kind of using chromatographic sheet as the micro-fluidic chip of substrate, including chromatographic sheet substrate, and at least one microfluidic channel constructed in substrate surface.The production method of the micro-fluidic chip: successively stacking chromatographic sheet, wax film, three layers of transparent pressing plate and clamp, is placed in the lower section of laser engraving machine laser head, and laser beam flying goes out designed microfluidic channel pattern；Laser beam melts the hydrophobic waxy material on wax film and is transferred to the stationary phase layer of chromatographic sheet, cools and solidifies immediately, constructs out microfluidic channel；It is cut into the region of microfluidic channel, obtains the micro-fluidic chip using chromatographic sheet as substrate.The utility model makes full use of the chromatographic isolation characteristic of thin-layer chromatography plate surface, and using it as the micro-fluidic chip of substrate preparation, its fluid channel has TLC separation function, and analysis efficiency and accuracy can be improved.
Description
Technical field
The utility model belongs to the technical field of micro-fluidic chip, and in particular to a kind of using chromatographic sheet as the micro- of substrate
Fluidic chip.
Background technique
Micro-fluidic chip (Microfluidic chip) is a kind of based on manipulating in micro-meter scale space to fluid
The science and technology of feature is wanted, is had the miniature energy on several square centimeter chips of the basic function in the laboratories such as chemistry, biology
Power, the chip lab that is otherwise known as (Lab-on-a-Chip).At this stage, the micro-fluidic chip of dominant form is mostly by microchannel shape
At network, whole system is run through with controlled fluid, to realize the various basic functions of conventional chemical or biology laboratory, such as:
Sample introduction, mixing, reaction, separation and detection etc..The essential characteristic and sharpest edges of micro-fluidic chip are a variety of monotechnicses micro-
Flexible combination and integrated in small controllable platform.
The core of microfluidic chip technology is the selection of chip base material and the micro-processing technology of fluid channel.Chip
The selection principle of base material has: 1, having good chemistry and bio-compatible between chip base material and analysis working media
Property；2, there is good electrical insulating property and thermal diffusivity；3, there is good optical property；4, surface will have good modify
Property；5, manufacture craft is simple, material and low manufacture cost.Be usually used in make micro-fluidic chip base material mainly have glass,
Quartz, silicon wafer, PDMS polymer, paper etc..These materials respectively have advantage and disadvantage: glass and quartz material have good electric osmose property,
Excellent optical property, can to carry out surface modified, but it is difficult to obtain the big channel of depth-to-width ratio；Silicon sheet material has good
Thermal stability, good finish, processing technology are mature, but its frangible, expensive；PDMS polymer material can repeat
Reversible deformation, surface can carry out a variety of modifying and decoratings, but non-refractory, thermal coefficient are low；The cost of material is low for paper chip, biofacies
Capacitive is strong, but processes inconvenience, need to use a variety of special reagents or instrument and equipment.
The micro-fluidic chip of above-mentioned material preparation, fluid channel do not have TLC separation ability, i.e. " microfluidic chromatography
Column " function.In the analysis of the matrix complex sample such as food, environment, the separation and purification of test solution are important step, if can be
TLC separation function is realized in the fluid channel of micro-fluidic chip, then can greatly simplify sample pre-treatments step, is improved and is divided
Analyse efficiency and accuracy.
Micro-fluidic chip processing method based on above-mentioned material mainly have photoetching, etching, hot pressing, injection molding, laser ablation and
Surface modification etc..The above method is both needed to complete using the equipment of special expensive, complex steps, requires height to operation processing technology,
Processing cost is high, it is difficult to universal.And due to the micro-meter scale in micro-fluidic chip channel, also requiring during the preparation process must be to ring
Border air themperature, humidity, grain density carry out strict control.These requirements are generally required can be only achieved in toilet, clean
Room technology is inseparable with the success or failure of facture of microchip process, the toilet of a high standard need great number investment and compared with
High operating cost.
In view of above-mentioned limitation existing for current micro-fluidic chip base material and its processing method, the utility model proposes
It is a kind of using chromatographic sheet as micro-fluidic chip of substrate and preparation method thereof.Chromatographic sheet is that laboratory commonly separates material
Material, has characteristics that there is powerful chromatogram separating capacity on 1, surface, has " microfluidic chromatography in the solution channel of its surface construction
The function of column " is, it can be achieved that efficiently separating and purifying for solution；2, there is chemical inertness；3, the gap between surface particles is day
Right microchannel, solution rely on capillary flow, are not necessarily to additional driving force；4, surface is white, various chromogenic reaction comparisons
Obviously, it can be used as the good material of luminosity, Visual retrieval method；5, the chromatosheet containing fluorescent material can provide for ultraviolet detection
It is convenient；6, cheap and easy to get.
Summary of the invention
The utility model provide it is a kind of using chromatographic sheet as the micro-fluidic chip of substrate, to solve traditional material miniflow
Control chip upper fluid channel does not have the problem of TLC separation ability.
It is provided by the utility model a kind of using chromatographic sheet as the micro-fluidic chip of substrate, including chromatographic sheet, it is thin
The stationary phase that layer chromatography plate includes support plate, is coated on support plate, which is characterized in that chromatographic sheet is as substrate, in base
Bottom surface has constructed at least one microfluidic channel；The microfluidic channel freezes solidly on thin-layer chromatography by hydrophobic waxy material
The lines formed in the stationary phase of plate, which enclose, to be built.
Stationary phase material on support plate includes but is not limited to silica G, silica GF254, diatomite, aluminium oxide, crystallite fibre
Tie up element etc.；Support that plate material includes but is not limited to glass, plastics, aluminium sheet etc..
The chromatographic sheet, stationary phase coating layer thickness are no more than 250 μm.
The microfluidic channel includes the micro-fluidic chip basic functional units being sequentially communicated, include at least sample inlet pool,
TLC separation channel, reaction tank or detection cell, waste liquid pool.
The hydrophobic waxy material is the hydrophobic waxy material coated on paraffin paper or carbon paper or heat transfer printing carbon tape.
The utility model has the beneficial effects that using chromatographic sheet as the micro-fluidic chip of substrate, microfluidic channel has
TLC separation ability, i.e. " microfluidic chromatography column " function, can be separated and be purified to complex sample test solution, before capable of simplifying sample
Processing step improves analysis efficiency and accuracy.
Detailed description of the invention
The utility model is described in more detail with reference to the accompanying drawing.
Fig. 1 is chromatographic sheet, wax film, transparent pressing plate stack level schematic diagram.
Fig. 2 is successively to stack 3 layers of Fig. 1 and clamp schematic diagram.
Fig. 3 is that will stack and clamp the lower section that 3 finished are placed on laser engraving machine laser head, and laser beam is in transparent pressing plate
The good microfluidic channel schematic diagram of Scan Design on layer.
Fig. 4 be it is scanned, open transparent pressing plate and wax film, be cut into the region of microfluidic channel, obtain with
Chromatographic sheet is the micro-fluidic chip of substrate.
In figure: 1-chromatographic sheet, 2-wax films, 3-transparent pressing plates, 4-clips, 5-laser engraving machines, 6-
Laser head, 7-laser beams, 8-sample inlet pools, 9-TLC separation channels, 10-reaction tanks or detection cell, 11-waste liquid pools.
Specific embodiment
Below with reference to the attached drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out clear
Chu is fully described by.Obviously, the described embodiments are only a part of the embodiments of the utility model, rather than whole realities
Apply example.Based on the embodiments of the present invention, those of ordinary skill in the art institute without making creative work
The every other embodiment obtained, fall within the protection scope of the utility model.
As shown in connection with fig. 4, the utility model provides a kind of using chromatographic sheet as the micro-fluidic chip of substrate including thin
Layer chromatography plate 1, the chromatographic sheet 1 are the common city for thin-layer chromatographic analysis that stationary phase is coated on support plate
Chromatographic sheet is sold, chromatographic sheet 1 is used as substrate, has constructed at least one microfluidic channel in substrate surface；Described is micro-
Flow control channel, which is freezed solidly on the lines formed in the stationary phase of chromatographic sheet 1 by hydrophobic waxy material, encloses and builds.
Stationary phase material includes but is not limited to silica G, silica GF254, diatomite, aluminium oxide, microcrystalline cellulose etc.；It supports
Plate material includes but is not limited to glass, plastics, aluminium sheet etc..
The chromatographic sheet 1, stationary phase coating layer thickness are no more than 250 μm.
The microfluidic channel is the micro-fluidic chip basic functional units being sequentially communicated, and includes at least sample inlet pool 8, thin
Layer chromatography split tunnel 9, reaction tank or detection cell 10, waste liquid pool 11.
The hydrophobic waxy material is the hydrophobic waxy material coated on paraffin paper or carbon paper or heat transfer printing carbon tape.
Referring to figs. 1 to 4, the micro-fluidic chip preparation method the following steps are included:
(1) chromatographic sheet 1, wax film 2, transparent pressing plate 3 are obtained；
(2) such as Fig. 1 and Fig. 2, wax film 2 is flattened to the stationary phase layer surface for being covered in chromatographic sheet 1, by transparent pressure
Plate 3 covers the surface in wax film 2.Surrounding is clamped transparent pressing plate 3, wax film 2,1 three layers of chromatographic sheet with clip 4,
Middle layer wax film 2 fold should not occurs and arch upward；
(3) such as Fig. 3, the three levels that step (2) clamp are placed in the lower section of 5 laser head 6 of laser engraving machine, transparent pressure
Plate 3 is located at top layer, and enables laser beam 7 perpendicular to transparent pressing plate 3；
(4) start laser engraving machine 5, laser head 6 launch laser beam 7 scanned on 3 layers of transparent pressing plate it is designed
Microfluidic channel pattern；It, can be micro- with one-off scanning one according to the size of wax film 2 and the area size of microfluidic channel
Flow control channel pattern or multiple identical or different microfluidic channel patterns；To make one or more micro-fluidic chips；
(5) such as Fig. 4, transparent pressing plate 3 and wax film 2 is opened, the region of microfluidic channel is cut into, is obtained with thin layer color
Compose the micro-fluidic chip that plate is substrate.
Wax film 2 is paraffin paper or carbon paper or heat transfer printing carbon tape in step (1).
Transparent pressing plate 3 is sheet glass or quartz plate in step (1), and thickness is no more than 0.5 mm, cannot have macroscopic
The defects of grains of sand, bubble, fold.
The power of laser beam 7 is 300~1000 mW in step (4)；Scanning speed is 800~1500 mm/min.
Laser beam 7 penetrates transparent pressing plate 3 and is irradiated on wax film 2 in step (4), and the heat of luminous point makes wax film 2
On hydrophobic waxy material instant melting, the hydrophobic waxy material of fusing immediately by 1 stationary phase layer of chromatographic sheet absorb
And cool and solidify immediately, it is formed on " hydrophobic dam ", constructs out microfluidic channel.
Microfluidic channel pattern can be designed using computer drawing software in step (4).
Embodiment 1
It is a kind of using silica GF254 glass base chromatographic sheet as the facture of microchip method of substrate, specific steps are as follows:
(1) selection stationary phase is silica GF254, the chromatographic sheet that support plate is glass is substrate, stationary phase coating
With a thickness of 200 μm；Selection stencil paper is wax film；The quartz plate for selecting 0.5 mm thickness is transparent pressing plate, quartz plate surface
It is smooth bright and clean, the defects of the grains of sand that are visible by naked eyes, bubble, fold；
(2) stencil paper flattening is covered in the stationary phase layer surface of chromatographic sheet, quartz plate is covered in stencil paper
Surface.With clip by quartz plate, stencil paper, three layers of chromatographic sheet clamping, middle layer stencil paper surrounding should not occur
It fold and arches upward；
(3) three levels that step (2) clamp are placed in the lower section of laser engraving machine laser head, quartz plate is located at most upper
Layer, and enable laser beam perpendicular to quartz plate；
(4) start laser engraving machine, laser head launch laser beam scanned on quartz plate 2 it is identical designed
Microfluidic channel pattern；Laser beam power is 800 mW；Scanning speed is 1000 mm/min；
(5) quartz plate and stencil paper are opened, the region of microfluidic channel is cut into, is obtained with silica GF254 glass base
Chromatographic sheet is the micro-fluidic chip of substrate.
Embodiment 2
It is a kind of using microcrystalline cellulose F254 aluminium base chromatographic sheet as the facture of microchip method of substrate, specific steps
Are as follows:
(1) selection stationary phase is microcrystalline cellulose F254, the chromatographic sheet that support plate is aluminium sheet is substrate, stationary phase
Coating layer thickness is 100 μm；Selection carbon paper is wax film；The quartz plate for selecting 0.5 mm thickness is transparent pressing plate, quartz plate table
The defects of face is smooth bright and clean, the grains of sand that are visible by naked eyes, bubble, fold；
(2) carbon paper flattening is covered in the stationary phase layer surface of chromatographic sheet, quartz plate is covered into the table in carbon paper
Face.For surrounding with clip by quartz plate, carbon paper, three layers of chromatographic sheet clamping, fold and arch should not occur in middle layer carbon paper
It rises；
(3) three levels that step (2) clamp are placed in the lower section of laser engraving machine laser head, quartz plate is located at most upper
Layer, and enable laser beam perpendicular to quartz plate；
(4) start laser engraving machine, laser head launch laser beam scanned on quartz plate it is designed micro-fluidic logical
Road pattern；Laser beam power is 500 mW；Scanning speed is 1200 mm/min；
(5) quartz plate and carbon paper are opened, the region of microfluidic channel is cut into, is obtained with microcrystalline cellulose F254 aluminium base
Chromatographic sheet is the micro-fluidic chip of substrate.
The utility model is a kind of using chromatographic sheet as the production method of the micro-fluidic chip of substrate and commonly used laser
Similar place has been seen on the method surface that ablation prepares micro-fluidic chip, and essence is completely different, and purpose is also entirely different.Commonly
Laser ablation method is that ablation goes out directly on the base materials such as metal, plastics, glass, ceramics using powerful laser beam
Micro-fluidic chip.The disadvantage is that production efficiency is low, high power laser is expensive, and energy is big, there is certain danger.And this method
It may not apply on chromatographic sheet base material, reason has two: consolidating first is that powerful ablation cannot change chromatographic sheet
The hydrophilic, hydrophobic property for determining phase layer, cannot form thin dam structure, be unable to the directed flow of operative constraint liquid, to be unable to structure
Build out the basic functional units of micro-fluidic chip；Second is that the surface that powerful ablation can destroy chromatographic sheet stationary phase layer is special
Property, so that it is lost TLC separation ability.The utility model proposes method use small-power (≤1000 mW) laser beam
Transparent pressing plate scanning wax film is penetrated, the hydrophobic waxy material instant melting on wax film, the hydrophobic wax of fusing are made
Material is absorbed by chromatographic sheet stationary phase layer immediately and is cooled and solidified immediately, can be effectively formed thin dam structure, be constructed
Micro-fluidic chip basic functional units out.Laser not direct irradiation chromatographic sheet, will not destroy basic functional units stationary phase
The surface characteristic of layer, retains its TLC separation ability.
Claims (5)
1. a kind of using chromatographic sheet as the micro-fluidic chip of substrate, including chromatographic sheet, chromatographic sheet include support plate,
The stationary phase being coated on support plate, which is characterized in that chromatographic sheet has constructed at least one as substrate, in substrate surface
Microfluidic channel；The microfluidic channel is freezed solidly on the line formed in the stationary phase of chromatographic sheet by hydrophobic waxy material
Item, which encloses, to be built.
2. as described in claim 1 a kind of using chromatographic sheet as the micro-fluidic chip of substrate, which is characterized in that the thin layer
The stationary phase material of chromatosheet is silica G, silica GF254, diatomite, aluminium oxide, microcrystalline cellulose any one therein；Institute
The support plate material for stating chromatographic sheet is glass, plastics, aluminium sheet any one therein.
3. as claimed in claim 2 a kind of using chromatographic sheet as the micro-fluidic chip of substrate, it is characterised in that described is thin
Layer chromatography plate substrate, stationary phase coating layer thickness are no more than 250 μm.
4. as described in claim 1 a kind of using chromatographic sheet as the micro-fluidic chip of substrate, it is characterised in that described is micro-
Flow control channel includes the micro-fluidic chip basic functional units being sequentially communicated, include at least sample inlet pool, TLC separation channel,
Reaction tank or detection cell, waste liquid pool.
5. as described in claim 1 a kind of using chromatographic sheet as the micro-fluidic chip of substrate, it is characterised in that described hydrophobic
Property wax material be the hydrophobic waxy material coated on paraffin paper or carbon paper or heat transfer printing carbon tape.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201821199653.7U CN208786436U (en) | 2018-07-27 | 2018-07-27 | It is a kind of using chromatographic sheet as the micro-fluidic chip of substrate |
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| CN201821199653.7U CN208786436U (en) | 2018-07-27 | 2018-07-27 | It is a kind of using chromatographic sheet as the micro-fluidic chip of substrate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109174216A (en) * | 2018-07-27 | 2019-01-11 | 汉江师范学院 | It is a kind of using chromatographic sheet as micro-fluidic chip of substrate and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109174216A (en) * | 2018-07-27 | 2019-01-11 | 汉江师范学院 | It is a kind of using chromatographic sheet as micro-fluidic chip of substrate and preparation method thereof |
| CN109174216B (en) * | 2018-07-27 | 2024-02-13 | 汉江师范学院 | Microfluidic chip with thin-layer chromatographic plate as substrate and manufacturing method thereof |
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