CN208752008U - A kind of turntable and device in high pass measurement phosphorescence service life - Google Patents

Abstract

A kind of turntable and device in high pass measurement phosphorescence service life, it can be used for high-throughput phosphorescence lifetime measurement, measurement method be exciting light is focused on it is a branch of, at irradiation and disk edge one, when turntable is rotated by motor driven, the exciting light for being radiated at disk edge can be introduced into sample cell by the optical channel on turntable, for exciting sample, the sample of each sample slot be excited after in Space Rotating, it is spatially separated the luminous signal of different delays time, can get the phosphorescence service life of sample by camera photographic analysis.The method achieve use continuous light source while measuring phosphorescence service life of multiple samples；Since exciting light has higher power density, the sample of low concentration sample and weak light can be measured；Exciting light is limited in optical channel internal transmission, light source scattering background can be significantly reduced.Furthermore this method does not need the locking phase control of expensive light-pulse generator, high speed detector and complexity, has the characteristics that at low cost, instrument is simple portable.

Description

A kind of turntable and device in high pass measurement phosphorescence service life

Technical field

The present invention relates to the manufactures and application of the lifetime measurement method of luminescence generated by light and pertinent instruments device.Belong to optics instrument Device manufacture and instrument analysis field.

Background technique

The characteristics such as materials absorbed light, luminescence generated by light, chemiluminescence have very more applications in analysis detection field.Its principle It is the change that will lead to materials absorbed light degree, luminous intensity based on the change of the factors certain in environment mostly, by detecting luminous intensity Variation judge the changes of certain factors.However in actual measurement, the whether measurement of absorbance or luminous intensity, There are much relations with detector sensitivity；In the detection of luminescence generated by light, excitating light strength directly affects fluorescence and phosphorescence Intensity.In addition to this, instrument on the interference of the collecting efficiency of light, environment light, impurity shine etc. many factors can all influence to survey Measure result.Thus, the same sample, the result obtained using different apparatus measures all can be variant.

In order to exclude above-mentioned various errors, often obtained under same measuring condition using master sample and sample to be tested Result be compared, to exclude instrument error or preparation work curve, to realize the quantitative analysis of sample to be tested.In addition, Due to Aging equipment (such as light source dies down) factor, generally require repeatedly to be corrected instrument, these all directly result in behaviour It is cumbersome to make process, increases human error, reduces the repeatability of measurement result.In still further aspect, above-mentioned detection institute according to Bad instrument is mostly large-scale instrument, is not easy to detect (POCT) immediately.Although in recent years, developing many portable instruments Equipment or even many detections can be realized on the Intelligent hardwares such as mobile phone, but the essence of light intensity is also detected without changing, thus It still remains instrument and needs the problem of correcting.

Time-resolved luminous detection also have in analysis detection many applications (referenced patent CN201210215872.0, CN201610166288.9, CN201610416029.7).Wherein time-gated detection is after excitation by a delay Luminous intensity is detected again, and this method can remove short-life background fluorescence interference, have very high signal-to-noise ratio.However its Matter still detects light intensity, it is still desirable to rectify an instrument.

Another time-resolved detection is the luminescent lifetime for measuring fluorescence or phosphorescence, i.e. in the service life of excitation state, passes through The detection luminous service life judges the variation of certain factor in environment.A kind of method of detection luminescent lifetime is: using pulsed light Source excitation sample is changed with time by the luminous intensity that detector detects sample；The curve decayed at any time to light intensity into Row analysis, obtains the length in service life.In the attenuation curve of single index, the length in service life is defined as decaying to original 1/e institute Time (bibliography Anal. Chem., the 1990,62,270A -277A needed；Pure Appl. Chem. 2014, 86,1969-1998.).

The service life of excitation state is the intrinsic property of molecule, in most cases, the intensity of lifetime of excited state and exciting light It is unrelated, thus, the life value that the same sample measures in different instruments is almost the same, and there is no need to individually carry out school to instrument Just.Have very relative to detection light intensity using lifetime of excited state as detection according to error caused by instrument difference can be excluded High reliability.

Existing life tests have been applied in fluorescence lifetime imaging (bibliography Anal. Chem., 2017,89, 8104-8111) it, can be used for distinguishing the structure of cell, tissue.However it is widely applied to substance not yet based on life tests In analysis detection, it is most important the reason is that, expensive, such as the light-pulse generator, time correlation of the instrument in existing detection service life The detectors such as single photon counter, streak camera (with reference to Chinese patent CN201410353200.5, CN201310694918.6, CN201310027775.3).The equipment of still further aspect, above-mentioned various measurement service life is difficult to accomplish Small-sized, portability, this all limits the application and development of life tests.

In addition, one-shot measurement can only often detect the service life of a sample, it is impossible to be used in high pass in existing life tests The fluorescence lifetime of amount detects.This is primarily due to the existing detector such as single photon counter, streak camera etc. for surveying the service life, it is difficult to Different samples are distinguished from spatial domain.Some special imaging sensors, such as single-photon avalanche diode were developed in recent years (SADP) array, recordable phase (bibliography M. Gersbach, et al., the Proc. SPIE such as cmos sensor 2010, 7780, 77801H；R. Franke, G. A. Holst, Proc. SPIE 2015,9328,93281K), it can The fluorescence lifetime for measuring multiple pixels simultaneously has faster image taking speed in global fluorescence lifetime imaging.However, this A little detectors also need to cooperate light-pulse generator, locking phase control that could use.

The present inventor discloses in patent (CN201711110647.X) previous a kind of to be measured using imaging sensor The method in service life, the sample being stimulated using motor driven make the luminous signal of different delays time in space in Space Rotating Time-domain information is converted to spatial-domain information and detected by upper separation, realizes the synchro measure in multiple sample fluorescence service life.It should Method does not need pulse and locking phase control, has ultralow cost.However, in high-throughput lifetime measurement, it is more in order to realize Point excitation, needs the LED light of multiple laser faculas or diverging to focus on turntable.This aspect will cause scattering interference；In addition On the one hand, for needing strong light activated sample, dispersion laser or LED beam directly reduce excitation light power density, difficult To detect low concentration or micro sample.Although may insure excitation light intensity using the point-to-point exciting light of multiple lasers, It will increase the complexity and cost of instrument.

Summary of the invention

This patent is a kind of important improvement of patent (CN201711110647.X), the sample being all stimulated using motor driven This is spatially separated the luminous signal of different delays time, time-domain information is converted to spatial-domain information in Space Rotating It is detected.The theing improvement is that of this patent focuses on exciting light a branch of, makes excitation beam from the flat of sample Plane of rotation Line direction excites sample, and the rotational trajectory of samples all in this way can all intersect with excitation beam, i.e., sample in rotary course all It can stimulated luminescence excitation.This method only uses a light source, not only ensure that the energy density of light beam, but also can be used for high throughput Fluorescence lifetime measurement, relative to previous patent have significant progress.

To achieve the above object, this patent discloses a kind of for measuring the turntable in phosphorescence service life, it is characterised in that:

There are multiple sample cells on turntable, the distance in each sample slot distance turntable center of circle differs, and each sample cell connects one Optical channel, all optical channels are distributed on turntable, and each optical channel is respectively independent, are not interfere with each other mutually；

Optical channel other end connects disk edge, and the exciting light for that will be radiated at disk edge introduces optical channel, and Excite the sample in sample cell；

The medium for constituting optical channel is selected from air, optical fiber, capillary.

In a kind of preferred turntable, optical channel medium is multimode fibre.

In another preferred turntable, optical channel medium is air, and optical channel inner wall has reflectance coating.

In another preferred turntable, optical channel medium is transparent capillary, and capillary is used as sample cell simultaneously.

Based on above-mentioned various turntables, this patent discloses a kind of phosphorescence lifetime measurement device, it is characterised in that:

It further include motor, light source, camera including any one turntable in above-mentioned various turntables,

Wherein, turntable is even driven by motor shaft, and the light that light source issues is radiated on the certain point of disk edge, camera Camera lens is parallel with turntable, for shooting the picture of turntable.

A kind of preferred lifetime measurement device, further includes collector lens between light source and turntable, for gathering the light of light source On coke to the certain point of disk edge.Excitation light utilization efficiency can be improved in this way, enhance fluorescence signal.

A kind of preferred lifetime measurement device, it is characterised in that: light source is selected from laser diode or LED light source.

A kind of preferred lifetime measurement device, it is characterised in that: optical channel medium is capillary；Liquid sample is surveyed When amount, liquid sample is placed in capillary, can be first centrifuged, then detect, and realizes centrifuge separation and the integration of life tests.

Another preferred lifetime measurement device, further includes imaging len between camera and turntable, for by turntable As amplification, then take pictures.

It preferably, further include optical filter before camera gun, for filtering out exciting light in above-mentioned all measuring devices.

Above-mentioned all devices can be used for detecting the phosphorescence service life.

Principle with above-mentioned various device measurement fluorescence lifetimes is as follows:

The light that light source issues is radiated at the edge somewhere of turntable, and during turntable rotation, light can enter turntable according to this Each optical channel, and sample in exciting light channel end sample cell shines,

Since turntable is to continue rotation, thus, only when the end of optical channel goes to light area, sample can be made Sample in this slot is excited；With the lasting rotation of turntable, the end of optical channel is gone to except light area, sample no longer by Illumination excitation, but the luminous of sample can decay with the time；If rotary speed is that at the uniform velocity, the luminous of sample can turn An arc of lighting is formed on disk, and arc length is directly proportional to delay time.

Due to there is a plurality of optical channel on turntable, thus multiple samples can be excited to form multiple arc of lightings, if to turntable into Row is taken pictures, then can recorde the picture of all arc of lightings emitted by the sample, each arc of lighting represent a sample it is luminous at any time The curve of decaying；

According to rotary speed, the delay time of every bit on arc of lighting can be calculated；

Brightness according to image slices vegetarian refreshments or gray value again, can calculate the relative luminous intensity of every bit on arc of lighting；

The curve that the luminous intensity of sample decays at any time is just so obtained, being fitted to the curve can be obtained by Service life.

A kind of such method is only obtained with the luminescent lifetime of multiple samples by a photo, thus is high pass The lifetime measurement of amount.

Relative to the method in priority patent (CN201711110647.X), the advantage of this method is:

The light of single source is focused on a bit, while high pass measurement, ensure that the power density of exciting light, nothing Multiple light sources are needed, instrument is significantly simplified and are reduced costs；

Since exciting light has higher power density, the sample of low concentration sample and weak light can be measured；

Exciting light is limited in optical channel internal transmission, light source scattering background can be significantly reduced；

Exciting light is transmitted using multimode fibre, each sample can be made by substantially equicohesive light excitation, convenient for multiple The test of parallel samples.

Relative to existing phosphorescence lifetime measurement device, this patent the methods and apparatus disclosed are realized with stable state The service life of light source measurement excitation state has significant advantage:

Expensive light-pulse generator and detector are not needed, locking phase control is not needed, greatly reduces the cost of lifetime measurement；

All components can minimize, and can be made portable micromodule equipment, convenient for detection application immediately；

The luminescent lifetime that multiple samples can be detected simultaneously, improves the flux and efficiency of detection, and advantageously reduces and be System error.

Detailed description of the invention

Fig. 1 is a kind of lifetime measurement device, wherein 101 be turntable, 102 be sample cell, and 103 be optical channel, and 104 be electricity Machine, 105 be light source, and 106 be camera.

Fig. 2 is a kind of lifetime measurement device, wherein 201 be turntable, 202 be sample cell, and 203 be optical channel, and 204 be light-blocking Ring, 205 be motor, and 206 be light source, and 207 be reflector, and 208 be lens, and 209 be lens, and 210 be optical filter, and 211 be mobile phone.

Fig. 3 is a kind of turntable schematic top plan view, and 301 be sample cell, and 302 be optical channel.

Fig. 4 is a kind of turntable side view and schematic top plan view, and 401 be turntable, and 402 be transparent capillary, and 403 be loading slot, 404 be light hole.

Fig. 5 is a kind of turntable side view and schematic top plan view, and 501 be turntable, and 502 be capillary, and 503 be light hole.

Specific embodiment

In order to illustrate the principle of the present invention and its advantage, the present invention is made below by specific embodiment further Illustrate, its object is to help to better understand the contents of the present invention, but these specific embodiments do not limit in any way Protection scope of the present invention.

Embodiment 1, a kind of turntable and lifetime measurement device

As shown in Fig. 1, turntable 101 has sample cell 102 above, and each sample cell connects an optical channel 103, and light is logical Road is air pipeline, and the laser that laser light source 105 projects is parallel with turntable 101, and when turntable rotation, laser can be successively Into each optical channel, and excite the sample in sample cell.Turntable 101 is by 104 axis of motor even driving rotation.

In the operating condition, turntable uniform rotation, laser open, every revolution, the sample of all sample cells all by Laser excitation is primary, also, the fluorescence of sample or phosphorescence discharge in rotary course, forms arc of lighting, the rotation of each sample cell Radius is different, thus the arc of lighting of each sample will not be overlapped, and be taken pictures using camera 106 to arc of lighting, that is, have recorded illuminated message.

Image is handled, available luminescent lifetime, processing method and patent (CN201711110647.X) unanimously, It is specific as follows:

The grayscale value of the pixel of arc of lighting is directly proportional to luminous intensity, can be used as relative luminous intensity,

Under the conditions of uniform rotation, arc of lighting arc length is directly proportional to delay time, it is known that the revolving speed of motor can calculate respectively The delay time of a pixel,

Decay of luminescence curve can be obtained to delay time mapping with pixel grayscale value, according to public affairs known in the art Formula and method can obtain luminous life value to curve matching.

In this embodiment, optical fiber is also can be used in optical channel.

According to same principle, the quantity of sample cell and optical channel can be increased on turntable, realize the same of more Multi-example When measure.

Compared with the method for priority patent (CN201711110647.X), this method exciting light energy is concentrated at one, tool Have higher power density, can excite lower concentration sample and it is weaker shine, every revolution, each sample is swashed in turn Hair, improves the utilization rate of exciting light, moreover, the excitation light intensity that each sample cell receives is roughly equal, it can between each sample With comparison.In addition, optical channel is embedded in turntable, scattering light interference is greatly reduced.

Embodiment 2, a kind of turntable and lifetime measurement device

As shown in Fig. 2, turntable 201 has sample cell 202 above, and the radius of turn of each sample cell is different, each sample Slot all connects an optical channel 203, and optical channel is multimode fibre, leads to disk edge, and the light that light source 206 issues passes through reflector 207 reflections, are then focused on the edge of turntable 201 by lens 208；204 be baffle vane, for blocking exciting light scattering；Turntable 201 by 205 axis of motor even driving rotation；Lens 209, optical filter 210, mobile phone 211 constitute camera system, for carrying out to arc of lighting It takes pictures.

The method that the device measures the service life is consistent in embodiment 1 with principle.

Imaging can be amplified to arc of lighting, due to arc of lighting length generation by the part of turntable as amplification using lens 209 Table delay time, under same rotating speed, amplified arc of lighting is recorded by more pixels, thus has higher time resolution Rate can measure shorter luminescent lifetime.

Optical filter 210 can filter out the light for scattering light and non-measured wavelength, the signal-to-noise ratio of enhancing arc of lighting imaging.

Whole device can accomplish to minimize, and be easy to use mobile phone photograph.

Embodiment 3, a kind of turntable

The primary structure top view of turntable is as shown in Fig. 3, and 301 be sample cell, and 302 be optical channel, and optical channel is by optical fiber It constitutes, connects sample cell and disk edge, the light of disk edge can be imported into sample cell.The turntable can replace implementation Turntable in example 1 and 2 is used for lifetime measurement.

The turntable has given full play to the flexible advantage of optical fiber, any point on turntable can be established with disk edge non- The light-path of straight line.

Embodiment 4, a kind of turntable

As shown in Fig. 4, it is 2 layers that turntable 401, which divides, and 402 be transparent capillary, and 403 be loading slot, and 404 be light hole, Turntable internal layer is distributed with mostly with transparent capillary 402, and every capillary connects loading slot 403 and turntable side similar to a s type Edge can enter capillary due to capillarity with automatic sucking liquid sample, capillary itself can be used as waveguide, by turntable At the light at edge is imported into where liquid sample, and swashs sample and shine.

In turntable rotary course, and due to centrifugal force, liquid sample is made to be limited in the turning angle of capillary；Turntable it is upper Layer is opaque, but has multiple light holes 404, and the respectively turning angle of one capillary of mapping, and such liquid sample shines Luminous arc can be formed, also, the radius of turn of the turning angle of each capillary is different, thus each arc of lighting is not overlapped.

The turntable can replace the turntable in embodiment 1 and 2, be used for lifetime measurement.

The embodiment has given full play to the guide properties of capillary, capillary principle, while also using centrifugal force.

This method can be used for centrifuge separation and the lifetime measurement of mixture.

Embodiment 5, a kind of turntable

As shown in Fig. 5, it is 2 layers that turntable 501, which divides, and upper layer is opaque, has multiple light holes 503；Lower layer is embedded with U-shaped Bright capillary 502, U-shaped capillary apertures are directed toward the turntable center of circle, and disk edge is led in bottom, for importing the light of disk edge Into capillary, the sample in pipe is allow to be excited；The corresponding capillary of each light hole, makes a bit of of capillary Light can be appeared out of turntable, thus when turntable rotation, luminous arc can be formed, and not due to the radius of turn of each light hole Together, each arc of lighting track will not be overlapped, thus can be used for the service life luminescent lifetime detection of multiple samples.

The turntable can replace the turntable in embodiment 1 and 2, be used for lifetime measurement.

In the turntable, capillary is used as sample cell, and for leaded light；Also, turntable can generate centrifugal force when rotating, and make The different component of some samples can separate in U-shaped capillary, achieve the purpose that separation detection.

As embodiment 4, which has given full play to the guide properties of capillary, capillary principle, simultaneously also Centrifugal force is utilized.

This method can be used for centrifuge separation and the lifetime measurement of mixture.

Claims (10)

1. a kind of turntable in high pass measurement phosphorescence service life, it is characterised in that:

There are multiple sample cells on turntable, the distance in each sample slot distance turntable center of circle differs, and it is logical that each sample cell connects a light Road, all optical channels are distributed on turntable, and each optical channel is respectively independent, are not interfere with each other mutually；

Optical channel other end connects disk edge, and the exciting light for that will be radiated at disk edge introduces optical channel, and excites Sample in sample cell；

The medium for constituting optical channel is selected from air, optical fiber, capillary.

2. the turntable in high pass measurement phosphorescence service life as described in claim 1, it is characterised in that: optical channel medium is more Mode fiber.

3. the turntable in high pass measurement phosphorescence service life as described in claim 1, it is characterised in that: optical channel medium is sky Gas, and optical channel inner wall has reflectance coating.

4. the turntable in high pass measurement phosphorescence service life as described in claim 1, it is characterised in that: optical channel medium is Bright capillary, capillary are used as sample cell simultaneously.

5. a kind of device in high pass measurement phosphorescence service life, it is characterised in that: including any as described in claim 1-4 A kind of turntable further includes motor, light source, camera；

Wherein, turntable is even driven by motor shaft, and the light that light source issues is radiated on the certain point of disk edge, the camera lens of camera It is parallel with turntable, for shooting the picture of turntable.

6. the device in high pass measurement phosphorescence service life as claimed in claim 5, it is characterised in that: further include that optically focused is saturating Mirror, for the light of light source to be focused on to the certain point of disk edge.

7. the device in high pass measurement phosphorescence service life as claimed in claim 5, it is characterised in that: light source is selected from laser two Pole pipe or LED light source.

8. the device in high pass measurement phosphorescence service life as claimed in claim 5, it is characterised in that: optical channel medium is hair Tubule；When measuring to liquid sample, liquid sample is placed in capillary, can be first centrifuged, then detect, and realizes centrifuge separation With the integration of life tests.

9. the device in high pass measurement phosphorescence service life as claimed in claim 5, it is characterised in that: further include that imaging is saturating Mirror then is taken pictures for amplifying the picture of turntable.

10. the device in high pass measurement phosphorescence service life as claimed in claim 5, it is characterised in that: before camera gun It further include optical filter, for filtering out exciting light.