CN208699551U - A kind of cell cryopreservation kit - Google Patents
A kind of cell cryopreservation kit Download PDFInfo
- Publication number
- CN208699551U CN208699551U CN201821425261.8U CN201821425261U CN208699551U CN 208699551 U CN208699551 U CN 208699551U CN 201821425261 U CN201821425261 U CN 201821425261U CN 208699551 U CN208699551 U CN 208699551U
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- CN
- China
- Prior art keywords
- liquid nitrogen
- cuvette groove
- electric pushrod
- lid
- box body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 128
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 64
- 238000005192 partition Methods 0.000 claims abstract description 23
- 238000009434 installation Methods 0.000 claims abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000001816 cooling Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 18
- 230000005611 electricity Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000010687 lubricating oil Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses a kind of cell cryopreservation kits, including box body, first motor and the 4th electric pushrod, lid is installed at the top of the box body, the bottom end of lid is uniformly equipped with liquid nitrogen outlet, the bottom end of lid portion inside is equipped with liquid nitrogen distribution grid, and liquid nitrogen distribution grid and liquid nitrogen outlet connect, the other side of the first partition is separately installed with the second cuvette groove and third cuvette groove, the first fixed frame is all installed on the inner sidewall at both ends inside second cuvette groove and third cuvette groove, installation all has the first test tube inside second cuvette groove and third cuvette groove, and first test tube be all detachably connected with the first fixed frame, the utility model is connect by the input terminal in liquid nitrogen pump with liquid nitrogen case, and the output end of liquid nitrogen pump is connect with liquid nitrogen distribution grid, lid is installed at the top of box body, the bottom end of lid is uniformly equipped with liquid nitrogen outlet, it is real Show through liquid nitrogen come the advantages of cooling down to tray interior, while embodying safety and the feature of environmental protection.
Description
Technical field
The utility model relates to freeze kit technical field, specially a kind of cell cryopreservation kit.
Background technique
Fat stem cell, that is, adipose-derived mescenchymal stem cell be in recent years from adipose tissue it is isolated it is a kind of still not
The mature multipotential cell with the of self-replication capacity.Under certain condition, it can be divided into multiple functions cell, have
Regenerate the potential function of various histoorgans.
In the passage of cell culture and daily maintenance process, in terms of culture utensil, culture solution and various preparations all
It need to largely expend, and cell once leaves living body and starts originally culture, its various biological natures all will gradually become
Change and constantly has new variation with the increase of passage number and the variation of vitro condition.Therefore cell jelly is carried out in time
It deposits very necessary.Cell freezing, which is stored in -70 DEG C of refrigerators, can save 1 year as long as；Cell storage in liquid nitrogen, temperature up to-
196 DEG C, theoretically storage time is unlimited.
Utility model content
The purpose of this utility model is to provide a kind of lubricating oil mixing devices for having heating function, to solve above-mentioned background
The cell proposed in technology once leaves living body and starts originally culture, its various biological natures all will gradually change and with
The increase of passage number and the variation of vitro condition and constantly have new variation.Therefore cell cryopreservation is carried out very in time
It is necessary.Cell freezing, which is stored in -70 DEG C of refrigerators, can save 1 year as long as；For cell storage in liquid nitrogen, temperature reaches -196 DEG C,
The problems such as theoretically storage time is unlimited.
To achieve the above object, the utility model provides the following technical solutions: a kind of cell cryopreservation kit, including box
Body, lid, the first cuvette groove, first motor and the 4th electric pushrod are equipped with lid, the bottom end of lid at the top of the box body
Liquid nitrogen outlet is uniformly installed, the bottom end of lid portion inside is equipped with liquid nitrogen distribution grid, and liquid nitrogen distribution grid and liquid nitrogen outlet connect,
The tray interior is equipped with first partition and second partition, and the top of first partition is equipped with temperature sensor, first partition
Side be separately installed with the first pallet and the second pallet, and the first test tube is all installed inside the first pallet and the second pallet
Slot, the other side of the first partition are separately installed with the second cuvette groove and third cuvette groove, the second cuvette groove and third test tube
First fixed frame is all installed, installation all has first inside the second cuvette groove and third cuvette groove inside slot on the inner sidewall at both ends
Test tube, and the first test tube is all detachably connected with the first fixed frame, the box body bottom end of the second partition side is equipped with first
Electric pushrod and the second electric pushrod, the output end of the first electric pushrod are connect with the second cuvette groove, and the second electric pushrod
Output end is connect with third cuvette groove, and the bottom outside first pallet and the second pallet is separately installed with the 4th electric pushrod
With third electric pushrod, the bottom end inside the first pallet and the second pallet is separately installed with first motor and the second motor, and
The output end of one motor and the second motor is all connected with connecting rod, and the top of the box portion outside side is equipped with fixed case, Gu
Determine the bottom end inside case and be provided with liquid nitrogen case, and liquid nitrogen pump, the box portion outside are installed inside the fixed case on liquid nitrogen case top
Top far from fixed case side is equipped with controller, and controller passes through conducting wire and the first electric pushrod of liquid nitrogen pump, second electronic
Push rod, third electric pushrod, the 4th electric pushrod, first motor and the electrical connection of the second motor, and the output end of temperature sensor
It is electrically connected by the input terminal of conducting wire and controller.
Preferably, the input terminal of the liquid nitrogen pump is connect with liquid nitrogen case, and the output end of liquid nitrogen pump and liquid nitrogen distribution grid connect
It connects.
Preferably, the top of the box body side is equipped with installing tache, and installing tache is connect with lid.
Preferably, the bottom of the lid is equipped with the second inner cover, and the bottom of the second inner cover is connect with the first cuvette groove.
Preferably, the connecting rod middle position is all equipped with the second fixed frame, and detachable peace inside the second fixed frame
Equipped with the first cuvette groove.
Preferably, the top of first test tube is all equipped with the first inner cover, and the top of the first inner cover is connect with lid.
Compared with prior art, the utility model has the beneficial effects that cell cryopreservation kit in this, by liquid nitrogen pump
Input terminal connect with liquid nitrogen case, and the output end of liquid nitrogen pump is connect with liquid nitrogen distribution grid, and lid is equipped at the top of box body, lid
The bottom end of body be uniformly equipped with liquid nitrogen outlet, realize by liquid nitrogen come to tray interior cool down, while embody safety and
The advantages of feature of environmental protection, is separately installed with the 4th electric pushrod and third electricity by the bottom outside the first pallet and the second pallet
Push rod is moved, the bottom end inside the first pallet and the second pallet is separately installed with first motor and the second motor, may be implemented first
The rotation of pallet and the second pallet realizes the hommization of device and can to take out convenience of test tube etc. from all directions excellent
Point, the utility model are separately installed with the second cuvette groove and third cuvette groove, the second test tube by the other side in first partition
First fixed frame is all installed, inside the second cuvette groove and third cuvette groove inside slot and third cuvette groove on the inner sidewall at both ends
Installation all has the first test tube, and the first test tube is all detachably connected with the first fixed frame, the box body bottom of the second partition side
End is equipped with the first electric pushrod and the second electric pushrod, and the output end of the first electric pushrod is connect with the second cuvette groove, and the
The output end of two electric pushrods is connect with third cuvette groove to be realized and can be risen automatically by electric power by test tube, and device is embodied
Novelty, and improve efficiency and save the time, while increasing safety.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the utility model side side structure schematic view；
Fig. 3 is the utility model other side side structure schematic view；
Fig. 4 is the system block diagram of the utility model.
In figure: 1, box body；2, the first pallet；3, installing tache；4, lid；5, liquid nitrogen exports；6, the second pallet；7, the first examination
Tube seat；8, first partition；9, second partition；10, the second cuvette groove；11, third cuvette groove；12, liquid nitrogen pump；13, fixed case；
14, liquid nitrogen case；15, the first fixed frame；16, distribution grid；17, the first electric pushrod；18, the second electric pushrod；19, third is electronic
Push rod；20, the 4th electric pushrod；21, controller；22, the first test tube；23, the first inner cover；24, temperature sensor；25, second
Inner cover；26, connecting rod；27, the second motor；28, the second fixed frame；29, first motor.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-4 is please referred to, a kind of embodiment provided by the utility model: a kind of cell cryopreservation kit, including box body 1,
Lid 4, the first cuvette groove 7, first motor 29 and the 4th electric pushrod 20, the top of box body 1 are equipped with lid 4, the bottom of lid 4
Portion is equipped with the second inner cover 25, and the bottom of the second inner cover 25 is connect with the first cuvette groove 7, and the bottom end of lid 4 is uniformly equipped with
Liquid nitrogen exports 5, and the bottom end inside lid 4 is equipped with liquid nitrogen distribution grid 16, and liquid nitrogen distribution grid 16 is connect with liquid nitrogen outlet 5, box
First partition 8 and second partition 9 are installed, the top of first partition 8 is equipped with temperature sensor 24, which passes inside body 1
The model of sensor 24 can be DHT11 humidity sensor, and the side of first partition 8 is separately installed with the first pallet 2 and the second pallet
6, and the first cuvette groove 7 is all installed inside first pallet 2 and the second pallet 6, the other side of first partition 8 is separately installed with
Two cuvette grooves 10 and third cuvette groove 11 are all equipped on the inner sidewall at 11 inside both ends of the second cuvette groove 10 and third cuvette groove
First fixed frame 15, installing all inside the second cuvette groove 10 and third cuvette groove 11 has the first test tube 22, the top of the first test tube 22
Portion is all equipped with the first inner cover 23, and the top of the first inner cover 23 is connect with lid 4, and the first test tube 22 all with the first fixed frame
15 are detachably connected, and 1 bottom end of box body of 9 side of second partition is equipped with the first electric pushrod 17 and the second electric pushrod 18, should
The model of first electric pushrod 17 and the second electric pushrod 18 can be the vertical electric push rod of DYTZ1000-/110, and first electronic pushes away
The output end of bar 17 is connect with the second cuvette groove 10, and the output end of the second electric pushrod 18 is connect with third cuvette groove 11, the
Bottom outside one pallet 2 and the second pallet 6 is separately installed with the 4th electric pushrod 20 and third electric pushrod 19, the 4th electricity
The model of dynamic push rod 20 and third electric pushrod 19 can be the vertical electric push rod of DYTZ1000-/110, the first pallet 2 and the second support
Bottom end inside disk 6 is separately installed with first motor 29 and the second motor 27, the model of the first motor 29 and the second motor 27
It can be Y315M-2 motor, and the output end of first motor 29 and the second motor 27 is all connected with connecting rod 26, among connecting rod 26
Position is all equipped with the second fixed frame 28, and 28 inside of the second fixed frame is detachably equipped with the first cuvette groove 7,1 side of box body
Top installing tache 3 is installed, and installing tache 3 is connect with lid 4, and the top of 1 outside side of box body is equipped with fixed case 13, Gu
Determine the bottom end inside case 13 and be provided with liquid nitrogen case 14, becoming better for the liquid nitrogen pump 14 can be for 3H-000LN liquid nitrogen pump, and liquid nitrogen case 14
Liquid nitrogen pump 12 is installed inside the fixed case 13 on top, the input terminal of liquid nitrogen pump 12 is connect with liquid nitrogen case 14, and liquid nitrogen pump 12
Output end is connect with liquid nitrogen distribution grid 16, and 1 outside of box body is equipped with controller 21 far from the top of 13 side of fixed case, the control
The model of device 21 can be FHR-211 controller, and controller 21 passes through 12 first the 17, second electricity of electric pushrod of conducting wire and liquid nitrogen pump
Dynamic push rod 18, third electric pushrod 19, the 4th electric pushrod 20, first motor 29 and the electrical connection of the second motor 27, and temperature passes
The output end of sensor 24 is electrically connected by the input terminal of conducting wire and controller 21.
Working principle: in use, being first turned on lid 4, cell being put into the first test tube 22 or the second test tube 27, will
First test tube is put into the second cuvette groove 10 and third cuvette groove 11, can also be put into cell in the first cuvette groove 7, be passed through
Controller 21 runs temperature sensor 24, monitors the temperature inside box body 1, can pass through liquid nitrogen pump 12 when temperature is higher
Liquid nitrogen in liquid nitrogen case 14 is input to inside box body 1 by the liquid nitrogen outlet 5 at 16 both ends of distribution grid, to reduce in box body 1
The temperature that temperature reaches suitable cell cryopreservation is that temperature sensor 24 can issue signal by the temperature in portion, and controller 21 makes liquid nitrogen
Pump 12 stops working, by it is in need need to take out test tube when, pushed away by the way that first electric pushrod of the control of controller 21 17 and second are electronic
Bar 18 rises the second cuvette groove 10 and third cuvette groove 11.Can take away test tube, while can also be controlled by controller 21
4th electric pushrod 20 and third electric pushrod 19 are lifted the first pallet 2 and the second pallet 6, while first motor 29 and the second electricity
The output end of machine 27 drives the second fixed frame 28 rotation in connecting rod 26, and operator can be made to take test tube from all directions.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
Claims (6)
1. a kind of cell cryopreservation kit, including box body (1), lid (4), the first cuvette groove (7), first motor (29) and the 4th
Electric pushrod (20), it is characterised in that: be equipped with lid (4) at the top of the box body (1), the bottom end of lid (4) is uniformly installed
There is liquid nitrogen to export (5), the internal bottom end of lid (4) is equipped with liquid nitrogen distribution grid (16), and liquid nitrogen distribution grid (16) goes out with liquid nitrogen
Mouth (5) connection, the box body (1) is internal to be equipped with first partition (8) and second partition (9), the top installation of first partition (8)
Have temperature sensor (24), the side of first partition (8) is separately installed with the first pallet (2) and the second pallet (6), and the first support
It is all equipped with the first cuvette groove (7) inside disk (2) and the second pallet (6), the other side of the first partition (8) is separately installed with
The inner sidewall at the internal both ends of the second cuvette groove (10) and third cuvette groove (11), the second cuvette groove (10) and third cuvette groove (11)
On be all equipped with the first fixed frame (15), installation all has the first test tube inside the second cuvette groove (10) and third cuvette groove (11)
(22), and the first test tube (22) is all detachably connected with the first fixed frame (15), the box body (1) of second partition (9) side
Bottom end is equipped with the first electric pushrod (17) and the second electric pushrod (18), the output end of the first electric pushrod (17) and the second examination
Tube seat (10) connection, and the output end of the second electric pushrod (18) is connect with third cuvette groove (11), first pallet (2) and
The external bottom of second pallet (6) is separately installed with the 4th electric pushrod (20) and third electric pushrod (19), the first pallet (2)
Internal bottom end is separately installed with first motor (29) and the second motor (27) with the second pallet (6), and first motor (29) and
The output end of second motor (27) is all connected with connecting rod (26), and the top of the external side of the box body (1) is equipped with fixed case
(13), the internal bottom end of fixed case (13) is provided with liquid nitrogen case (14), and peace inside the fixed case (13) on liquid nitrogen case (14) top
Equipped with liquid nitrogen pump (12), the external top far from fixed case (13) side of the box body (1) is equipped with controller (21), controller
(21) pass through conducting wire and (12) first electric pushrod (17) of liquid nitrogen pump, the second electric pushrod (18), third electric pushrod (19), the
Four electric pushrods (20), first motor (29) and the second motor (27) electrical connection, and the output end of temperature sensor (24) passes through
Conducting wire and the input terminal of controller (21) are electrically connected.
2. a kind of cell cryopreservation kit according to claim 1, it is characterised in that: the input terminal of the liquid nitrogen pump (12)
It is connect with liquid nitrogen case (14), and the output end of liquid nitrogen pump (12) is connect with liquid nitrogen distribution grid (16).
3. a kind of cell cryopreservation kit according to claim 1, it is characterised in that: the top of box body (1) side
It is equipped with installing tache (3), and installing tache (3) is connect with lid (4).
4. a kind of cell cryopreservation kit according to claim 1, it is characterised in that: install the bottom of the lid (4)
Have the second inner cover (25), and the bottom of the second inner cover (25) is connect with the first cuvette groove (7).
5. a kind of cell cryopreservation kit according to claim 1, it is characterised in that: connecting rod (26) middle position
It is all equipped with the second fixed frame (28), and the first cuvette groove (7) detachably is installed inside the second fixed frame (28).
6. a kind of cell cryopreservation kit according to claim 1, it is characterised in that: the top of first test tube (22)
It is all equipped with the first inner cover (23), and the top of the first inner cover (23) is connect with lid (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821425261.8U CN208699551U (en) | 2018-08-31 | 2018-08-31 | A kind of cell cryopreservation kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821425261.8U CN208699551U (en) | 2018-08-31 | 2018-08-31 | A kind of cell cryopreservation kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208699551U true CN208699551U (en) | 2019-04-05 |
Family
ID=65945735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821425261.8U Expired - Fee Related CN208699551U (en) | 2018-08-31 | 2018-08-31 | A kind of cell cryopreservation kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208699551U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495449A (en) * | 2019-09-23 | 2019-11-26 | 青岛大学附属医院 | A kind of kit that freezen protective induces multi-potent stem cell and method |
-
2018
- 2018-08-31 CN CN201821425261.8U patent/CN208699551U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110495449A (en) * | 2019-09-23 | 2019-11-26 | 青岛大学附属医院 | A kind of kit that freezen protective induces multi-potent stem cell and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200724 Address after: 213000 Room 101, building 17, No.118, Jingang Road, Kunlun Street, Liyang City, Changzhou City, Jiangsu Province Patentee after: Jiangsu Qinke Biological Research Center Co.,Ltd. Address before: 213023, Jiangsu province Changzhou Bell Tower District Xilin street, Zhang village, Shen Jiang Village, No. 128 Patentee before: CHANGZHOU XIANGTAI BIOTECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190405 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |