CN208607422U - Multiband LED fluorescence microscope - Google Patents
Multiband LED fluorescence microscope Download PDFInfo
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- CN208607422U CN208607422U CN201821111475.8U CN201821111475U CN208607422U CN 208607422 U CN208607422 U CN 208607422U CN 201821111475 U CN201821111475 U CN 201821111475U CN 208607422 U CN208607422 U CN 208607422U
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Abstract
The utility model discloses a kind of multiband LED fluorescence microscopes, including microscope body and multiband LED light source system, the multiband LED light source system is connect by connector sleeve with the fluorescent light source interface of the microscope body, multiband LED light source system includes the chassis LED, N number of LED chip and/or LED chip group；First circumference is set on the chassis LED, and N number of LED chip and/or LED chip group interval are laid on first circumference；Wherein, N number of LED chip and/or LED chip group include the wave band of n different central wavelengths, N >=n >=2, further include optical path subsystem and control subsystem, and control subsystem is used to control the first incidence zone of the long LED chip and/or LED chip group face optical path subsystem of selected standing wave.Multiband LED fluorescence microscope light-source system provided by the utility model may be implemented to replace automatically for the LED chip of selected wave band, easy to use.
Description
Technical field
The utility model relates to microscope device fields, glimmering particularly for can provide a variety of multiband LED for needing wavelength
Light microscope.
Background technique
Substance is almost launched to contrast simultaneously and penetrates the longer light of optical wavelength, and when incidence under the light irradiation of specific wavelength
The light that substance issues after light stops extinguishes immediately, and this light is known as fluorescence.Fluorescence microscope be exactly utilize substance fluorescent characteristic into
The optical microscopy of row observation.While fluorescence microscope retains different microscopical functions, moreover it is possible to carry out Fluirescence observation, have
Detection capability is high, can be carried out the characteristics of multiple coloring observation, is widely used in field of biomedicine.
Epifluorescence microscope by basic microscope plus epi-fluorescence lighting system (including fluorescent light source, excitation filter disc,
Two to components such as light splitting piece, barrier filter and lens) composition.Fluorescent illumination source is the critical component of fluorescence microscope, tradition
Fluorescence microscope light source mainly use high-pressure sodium lamp, there are service life short (200 hours or so), fever is serious, operation is multiple
The problems such as miscellaneous, expensive.LED light source is that 21 century new type light source in the market occur, with its compact, low energy consumption,
The advantages that response is fast, the service life is long, receives close attention in microscope field, obtains in single band excitation fluorescence microscope
It is widely applied.But LED light source is single narrow-band light source, if necessary to the excitation light source using multiple wave bands, must just be replaced
Light source, time-consuming, using being inconvenient.
Utility model content
When needing the LED excitation light source using multiple wave bands for the fluorescence microscope of the prior art, light source is needed replacing,
Time-consuming, inconvenient problem with use, and the utility model embodiment provides a kind of multiband LED fluorescence microscope, for selected
The LED chip of wave band may be implemented to replace automatically, easy to use.
Multiband LED fluorescence microscope provided by the embodiment of the utility model includes microscope body and multiband LED light
Source system, multiband LED light source system are connected by the fluorescent light source interface of connector sleeve and microscope body；
Multiband LED light source system, including the chassis LED, N number of LED chip and/or LED chip group；It is set on the chassis LED
The first circumference is set, N number of LED chip and/or LED chip group interval are laid on the first circumference；Wherein, N number of LED chip and/or
LED chip group includes the wave band of n different central wavelengths, N >=n >=2；
Multiband LED light source system further includes optical path subsystem, and optical path subsystem includes the first prism and lens barrel, the first rib
Mirror includes parallel first plane of incidence and the first exit facet, parallel the first reflecting surface and the second reflecting surface, the first reflecting surface and
Second reflecting surface is equipped with reflectance coating, first plane of incidence and the first reflecting surface and is arranged at 45 degree of angles, and the first exit facet and second is instead
Face is penetrated to be arranged at 45 degree of angles；First plane of incidence includes the first incidence zone, and the first incidence zone is set as the first reflecting surface and enters first
Penetrate the view field in face；First exit facet includes the first outgoing area, and the first outgoing area is set as the second reflecting surface in the first outgoing
The central area of lens barrel one end is arranged in the view field in face, the first outgoing area；
Multiband LED light source system further includes control subsystem, and control subsystem is used to control the long LED core of selected standing wave
The first incidence zone of piece and/or LED chip group face.
Multiband LED fluorescence microscope provided by the embodiment of the utility model, by being carried out to multiband LED light source system
It improves, the chassis LED is set, required LED chip or LED chip group are set around the first peripheral annular on the chassis LED, it can
The LED of central wavelength wave band required for microscope is arranged on the chassis LED with realizing.When needing to use some central wavelength
The LED chip of wave band perhaps LED chip group when by control subsystem realize that LED chip or LED chip group face first enter
Penetrate area.Connecting LED chip at this time, perhaps the power supply LED chip of LED chip group or chipset will generate required central wavelength
The light of wave band.The first incidence zone of light major part face at this time, i.e., most of light is by the first of vertical first plane of incidence
Incidence zone enters the first prism.Due to being coated with reflectance coating on the first reflecting surface, while the first reflecting surface and the first incidence zone are at 45
Angle setting is spent, incident ray will be changed direction of transfer when direct projection is to the first reflecting surface by the first reflecting surface, and incident ray will be with
The parallel direction of first plane of incidence is transmitted to the second reflecting surface, and changes the direction of transfer of light again by the second reflecting surface, warp
Light after change enters in lens barrel perpendicular to the first exit facet from the first outgoing area, since the first outgoing area is arranged in lens barrel
Central area, the light into lens barrel will be transmitted along lens barrel central area along lens barrel, and finally by LED chip or LED core
In the excitation light emission to the object for needing to irradiate of piece group transmitting.
The LED chip or chipset of multiple and different central wavelength wave bands are set on the chassis LED, also may include LED core
Piece and the LED chip group being made of LED chip, when needing which wave band, by the LED chip or chipset and the first incidence zone
It matches, re-assemblies the LED chip of needs again without removing unwanted LED chip, realize and replace automatically
It changes, uses and carry and is all very convenient.Meanwhile first prism exciting light is changed into direction of transfer realizing, reach needs
Except light incident direction, exciting light passes through multiple reflections inside the first prism, and every secondary reflection can all form virtual light source picture,
Multiple reflections form two-dimensional virtual light source matrix, to keep output light more uniform, achieve the effect that even light.
Preferably, optical path subsystem further includes lens group, and lens group is arranged in lens barrel, and it is convex that lens group includes at least first
Lens and the second convex lens, the first convex lens are arranged in lens barrel close to the first prism end, and are set as the first outgoing area and are located at the
In the focal length of one convex lens；Second convex lens is set as in lens barrel far from the first prism end.Wherein, the second convex lens includes convex-concave
Eyeglass and planoconvex lens, convex-concave eyeglass are arranged between planoconvex lens and the first convex lens.Since the first outgoing area is located at first
In the focal length of convex lens, the light emitted from the first outgoing area will realize the hair to output light under the action of the first convex lens
Effect is dissipated, then the light through dissipating is again transformed into the parallel rays of collimation by the second convex lens, output light can be increased
Range of exposures.Lens adjusting device can be respectively set on the first convex lens and the second convex lens, lens adjusting device can
To realize that the first convex lens and the second convex lens are longitudinally moved forward and backward along lens barrel, realize between the first convex lens and the first outgoing area
The adjustment of distance between the adjustment of distance and the second convex lens and the first convex lens.Can also only it be arranged on the second convex lens
Lens adjusting device, and the distance between the first convex lens and the first outgoing area preset one most according to microscopical performance
Excellent fixed value.
Preferably, the second circumference with the first circumferential concentric, LED chip and/or LED chip group are set on the chassis LED
Interval is laid on the first circumference and the second circumference；
Optical path subsystem includes the second prism, and the second prism includes parallel second plane of incidence and the second exit facet, parallel
Third reflecting surface and the 4th reflecting surface, third reflecting surface and the 4th reflecting surface are equipped with reflectance coating, second plane of incidence and third
Reflecting surface is arranged at 45 degree of angles, and the second exit facet and the 4th reflecting surface are arranged at 45 degree of angles；Second plane of incidence includes second incident
Area, the second incidence zone are set as third reflecting surface in the view field of second plane of incidence；Second exit facet includes the second outgoing area,
Second outgoing area is set as the 4th reflecting surface in the view field of the second exit facet, and the second outgoing area is arranged in lens barrel one end
Heart district domain；
Control subsystem is used to control the LED chip and/or LED chip group face for being located at set wavelength on the first circumference
First incidence zone, or control are located at the second incidence of LED chip and/or LED chip group face of set wavelength on the second circumference
Area.
Preferably, the central wavelength of each of LED chip and/or LED chip group LED chip or LED chip group
Wave band is different, and the angular separation such as LED chip and/or LED chip group are arranged on the first circumference, and the center of circle setting of the first circumference exists
At the center on the chassis LED.
Second prism and the first prism can be in line setting, i.e., using the center of circle of lens barrel as reference point, the first prism and
For two prisms respectively along the diameter setting of a lens barrel, the second prism and the first prism can also be at an angle of setting, i.e. the first prism
It is arranged respectively along a radius of lens barrel with the second prism, the first prism and the second prism can be set in one plane,
It can be separately positioned in two planes parallel with the cross section of lens barrel.Only the projection of the first prism and the second prism is needed not have
There is overlapping that can realize the effect of the utility model embodiment technical solution.Preferably, the first prism and the setting of the second prism
In the approximately the same plane parallel with lens barrel cross section, it is same to the first prism and the second prism to can be convenient the first convex lens in this way
When suitable distance is set, realize that the light issued simultaneously to the first outgoing area and the second outgoing area is adjusted.
Preferably, the first prism and the second prism are rhombic prism.
Wherein, the utility model embodiment provides two kinds of concrete implementation modes to control subsystem to realize LED core
The matching of piece or LED chip group and the first incidence zone or the second incidence zone.In first way, optical path subsystem further includes
Prism fixed plate, the first prism and the second prism are fixed in prism fixed plate, and prism fixed plate is connect with lens barrel, control subsystem
System includes first motor, and the first driving wheel is set as hollow the first follower, the first rotary encoder and control circuit, and first
Motor connects the shaft of the first driving wheel, and the first follower is fixedly connected with prism fixed plate, and the first driving wheel and first is passively
Wheel is connect by transmission band connection, the first rotary encoder with the shaft of the first driving wheel, the first rotary encoder and control electricity
The connection of road signal, control circuit control LED chip and/or LED chip group power circuit and first motor power circuit.
In second of specific embodiment of control subsystem, control subsystem includes the second motor, the second driving wheel, the
Two followers, the second rotary encoder and control circuit, the second motor connect the shaft of the second driving wheel, and the second follower turns
The axle center on the chassis axis connection LED, the second driving wheel and the second follower pass through transmission band connection, the second rotary encoder and second
The shaft of driving wheel connects, and the second rotary encoder is connect with control circuit signal, and control circuit controls LED chip and/or LED
Chipset power circuit and the second motor power circuit.
In the implementation of above two control subsystem, the first implementation is rotated by drive prism fixed plate,
To drive the first prism, perhaps the second prism rotates to realize that the LED chip of selected wave band or chipset enter with first
Penetrate the matched effect in area or the second incidence zone.In second of implementation, by driving the rotation of the chassis LED, to drive LED
Perhaps LED chip group rotates to realize that the LED chip of selected wave band or chipset enter with first LED chip on chassis
Penetrate the matched effect in area or the second incidence zone.Both of which can be very good to realize the automatic LED core for replacing selected wave band
Piece or chipset.The first motor of above two mode, the preferred stepper motor of the second motor, to preferably realize rotation angle
The accurate control of degree.Control subsystem can also be mainly to realize the LED of selected wave band using other implementations, purpose
The matched effect in chip or chipset and the first incidence zone or the second incidence zone.
It preferably, further include prism centers micromatic setting, prism centers micromatic setting is connect with prism fixed plate.Pass through control
Subsystem realizes the angle adjustment of prism fixed plate, and by prism centers micromatic setting, prism fixed plate may be implemented
Straight-line displacement adjustment in lens barrel cross-wise direction so that the LED chip of selected wave band or chipset and the first incidence zone or
Realize more accurate matching in the second incidence zone of person.
Preferably, six faces of rhombic prism are disposed as burnishing surface, and in rhombic prism except the first incidence zone, first go out
Reflective membrane is coated on all faces penetrated except area, the second incidence zone, the second outgoing area.By in addition to the first incidence zone,
Reflective membrane is arranged in two incidence zones, the first outgoing area, the second outgoing area, can make the light for entering the first prism or the second prism
More fully finally launch from the first outgoing area and the second outgoing area by mirror-reflection, reaches better even light effect.
Detailed description of the invention
Fig. 1 is multiband LED light source system structure diagram in the utility model embodiment；
Fig. 2 is LED chassis structure schematic diagram in the utility model embodiment；
Fig. 3 is rhombic prism structural schematic diagram in the utility model embodiment；
Fig. 4 is that light propagates schematic diagram in rhombic prism in the utility model embodiment；
Fig. 5 is lens group structure schematic diagram in the utility model embodiment；
Fig. 6 is lens group and prism, the chassis LED assembly structure diagram in the utility model embodiment；
Fig. 7 is the utility model embodiment control subsystem control flow schematic diagram.
In attached drawing: 100, the chassis LED；101, LED chip；102, LED chip group；103, common cathode；104, pin；200,
First prism；201, first plane of incidence；202, the first exit facet；203, the first reflecting surface；204, the second reflecting surface；210, mirror
Cylinder；220, the second prism；230, prism fixed plate；240, prism centers micromatic setting；310, first motor；320, first actively
Wheel；330, the first follower；340, the first rotary encoder；350, transmission belt；400, connector sleeve；410, cooling fin；420, axis
Hold seat；510, the first convex lens；520, the second convex lens；521, planoconvex lens；522, convex-concave eyeglass.
Specific embodiment
In the following, being described further in conjunction with attached drawing and specific embodiment to the utility model, it should be noted that
Under the premise of not colliding, it can be formed in any combination between various embodiments described below or between each technical characteristic new
Embodiment.
It includes microscope body and multiband LED light source that the utility model embodiment, which provides multiband LED fluorescence microscope,
System (including fluorescent light source, excitation filter disc, two are to components such as light splitting piece, barrier filter and lens) composition, wherein microscope master
Body uses the microscope device of existing maturation, such as including eyepiece, object lens, optical imaging assemblies, technical solutions of the utility model
To the setting of microscope body there is no limit.Microscope body is equipped with fluorescent light source interface, and multiband LED light source system passes through
Connector sleeve 400 is connect with fluorescent light source interface, realizes that the fluorescence to observation object excites.Multiband LED provided by the utility model
Fluorescence microscope, mainly realization is realized the excitation light source in access fluorescence microscope and is improved, so that it is more to provide a kind of energy offer
The LED fluorescence microscope of kind central wavelength wave band.Multiband LED fluorescence microscope provided by the embodiment of the utility model uses
Prism is completed at the same time the function of turning to even light, reduces costs, has saved space, realizing can root on a set of LED light source
According to the purpose for needing to adjust a variety of different wave length exciting lights of output, the number that single set light source integrates light sources with different wavelengths is greatly expanded
Amount, extends the service life of fluorescence microscope light source, easy to operate, has a wide range of application.
Embodiment 1:
Multiband LED fluorescence microscope provided by the embodiment of the utility model, including microscope body and multiband LED light
Source system, multiband LED light source system are connected by the fluorescent light source interface of connector sleeve and microscope body.The utility model mentions
The multiband LED fluorescence microscope of confession, which mainly passes through, improves multiband LED light source system, and realizing can be with by a set of light-source system
The exciting light of various wavelength required for providing.
As shown in Figures 1 to 6, multiband LED light source system provided by the embodiment of the utility model, including the chassis LED
100, N number of LED chip 101 and/or LED chip group 102；First circumference, N number of LED chip 101 are set on the chassis LED 100
And/or the interval of LED chip group 102 is laid on the first circumference；Wherein, N number of LED chip 101 and/or LED chip group 102 include
The wave band of n different central wavelengths, N >=n >=2.
Preferably, each of LED chip 101 and/or LED chip group 102 LED chip 101 or LED chip group 102
Central wavelength wave band it is different, the angular separation such as LED chip 101 and/or LED chip group 102 are arranged on the first circumference, the
The center of circle of one circumference is arranged at the center on the chassis LED 100.
Preferably, can also be arranged on the chassis LED 100 with the second circumference of the first circumferential concentric, LED chip 101 and/
Or the interval of LED chip group 102 is laid on the first circumference and the second circumference；
Wherein, N number of LED chip 101 and/or LED chip group 102 refer specifically to only be arranged in each installation site
Individual LED chip 101, also can be set the LED chip group 102 being made of more than two LED chips, can also be according to need
Individual LED chip 101 is set in some locations, LED chip group 102 is set on other positions.LED chip 101
Or the function of the quantity of LED chip group 102 realization according to required for the fluorescence microscope is configured.LED chip 101 or
LED chip group 102 is fixed on the chassis LED 100.This fixation can be permanent fixation, such as welding.This is practical new
In the embodiment that type embodiment provides, LED chip 101 or LED chip group 102 are fixed on LED by dismountable mode
It on chassis 100, such as is connected through a screw thread or is connected by a snap, to realize to LED chip 101 or LED chip group
102 replaceable or maintenance.
In a specific embodiment as shown in Figure 2,24 LED chips 101 or LED chip group is arranged in the chassis LED 100
102, as needed, LED chip 101 is separately provided in some, and some forms LED chip group 102 by multiple LED chips 101, so as to
Realize the synthesis of multiple wave bands, by the combination of multiple LED chips 101 to realize the exciting light of specific wavelength.Wherein, 24
LED chip 101 and LED chip group 102 have common common cathode 103, each LED chip 101 and LED chip group 102 are single
Solely setting pin 104 is realized by pin 104 and common cathode 103 to each LED chip 101 or LED chip group 102
Individually control.24 kinds or less than 24 kinds of differences are contained in wave band possessed by 24 LED chips 101 or LED chip group 102
The wave band of central wavelength covers ultraviolet band to infrared band.The LED chip 101 being arranged on the chassis LED 100 can be according to need
It is arranged.24 LED chips 101 or LED chip group 102 are separately positioned on the first circumference and the second circumference, each circumference
Setting 12, wherein on the second circumference in internal diameter based on one single chip, and in first it is provided circumferentially about with
Based on LED chip group 102.The utility model embodiment illustrates the case where two concentric circumferences are arranged in the chassis LED 100, such as
In described in Fig. 2,24 groups of LED chips 101 or LED chip group 102 can be set, met most fluorescence microscopes to sharp
Shine the demand of each wave band.But as needed, it can also also be arranged again and the third circumference of the first circumferential concentric, the 4th circle
Week, technical solution provided by the utility model are without limitation.Further, can also be arranged at the back side on the chassis LED 100
Cooling fin 410 realizes better heat dissipation effect.
Multiband LED light source system provided by the utility model further includes optical path subsystem, as shown in Figure 1, Figure 3, optical path
Subsystem includes the first prism 200 and lens barrel 210, and the first prism 200 includes parallel first plane of incidence 201 and the first exit facet
202, parallel the first reflecting surface 203 and the second reflecting surface 204, the first reflecting surface 203 and the second reflecting surface 204 are equipped with reflection
Film, first plane of incidence 201 and the first reflecting surface 203 are arranged at 45 degree of angles, and the first exit facet 202 and the second reflecting surface 204 are at 45
Spend angle setting；First plane of incidence 201 includes the first incidence zone, and the first incidence zone is set as the first reflecting surface 203 in the first incidence
The view field in face 201；First exit facet 202 includes the first outgoing area, and the first outgoing area is set as the second reflecting surface 204 the
The central area of 210 one end of lens barrel is arranged in the view field of one exit facet 202, the first outgoing area.
Further, when the chassis LED 100 is equipped with the second circumference, as shown in fig. 6, optical path subsystem includes the second prism
220, the second prism 220 includes second plane of incidence and the second exit facet, parallel third reflecting surface and the 4th reflecting surface in parallel,
Third reflecting surface and the 4th reflecting surface are equipped with reflectance coating, and second plane of incidence and third reflecting surface are arranged at 45 degree of angles, and second goes out
It penetrates face and the 4th reflecting surface is arranged at 45 degree of angles；Second plane of incidence includes the second incidence zone, and it is anti-that the second incidence zone is set as third
Face is penetrated in the view field of second plane of incidence；Second exit facet includes the second outgoing area, and the second outgoing area is set as the 4th reflection
In the view field of the second exit facet, the central area of 210 one end of lens barrel is arranged in the second outgoing area in face；
Correspondingly, more to be arranged if also setting up third circumference, the 4th circumference on the chassis LED 100 as needed
LED chip 101 or LED chip group 102, third prism is arranged in optical path subsystem accordingly, the 4th prism is realized to third circle
Week, the light deflecting of the 4th LED chip 101 or LED chip group 102 provided circumferentially about and even light.
Wherein, the first prism 200 includes at least the parallel plane of incidence and exit facet with the second prism 220, and in parallel
Two reflectings surface, while the plane of incidence and first reflecting surface are arranged at 45 degree of angles, exit facet and second reflecting surface are at 45 degree of angles
Setting.It can also include other faces, the other faces of prism are not required in technical solution provided by the utility model
To further include two faces, such as rhombic prism also may include more faces.Multiband LED fluorescence provided by the utility model is aobvious
In micromirror light source system, the first prism 200, the preferred rhombic prism of the second prism 220, as shown in Fig. 3, Fig. 4 and Fig. 6.Rectangle rib
Mirror can ideally realize that the first prism 200, the second prism 220 are required, and reach best steering and even light effect.For
The steering to light and even light effect are further realized, six faces of rhombic prism are disposed as burnishing surface, and in rhombic prism
Reflective membrane is coated on all faces in addition to the first incidence zone, the first outgoing area, the second incidence zone, the second outgoing area.Pass through
Reflective membrane is being set in addition to the first incidence zone, the second incidence zone, the first outgoing area, the second outgoing area, can make to enter the first rib
The light of mirror 200 or the second prism 220 more fully passes through mirror-reflection and finally sends out from the first outgoing area and the second outgoing area
It is shot out, reaches better even light effect.
Control subsystem is used to control the LED chip 101 and/or LED chip group for being located at set wavelength on the first circumference
102 the first incidence zones of face, or control are located at the LED chip 101 and/or LED chip group 102 of set wavelength on the second circumference
The second incidence zone of face.
The LED chip that Fig. 3, Fig. 4 show optical path subsystem to the steering working principle of light, on the chassis LED 100
101 perhaps one group of LED chip group 102 light that shines enter the first prism 200 or the second prism 220, in prism internal communication
During form Uniform Illumination, by reflectance coating 2 secondary reflections reach optical path adjust lens group, by optical path adjust lens group
Beam shaping is carried out, collimated illumination is formed, is exported by lens barrel 210 and connector sleeve 400 to fluorescence microscope.First prism 200
Effect with the second prism 220 is so that light is generated displacement without changing its direction, while playing the role of even light.
Multiband LED light source system provided by the embodiment of the utility model, by the way that the chassis LED 100 is arranged, on the chassis LED
Required LED chip 101 or LED chip group 102 are set around the first peripheral annular on 100, may be implemented microscope institute
The LED of central wavelength wave band is needed to be arranged on the chassis LED 100.When needing the LED chip using some central wavelength wave band
101 perhaps LED chip group 102 when by control subsystem realize that LED chip 101 or 102 face of LED chip group first enter
Penetrate area.Connecting LED chip 101 at this time, perhaps the power supply LED chip 101 of LED chip group 102 or LED chip group 102 will produce
The light of central wavelength wave band required for raw.The first incidence zone of light major part face at this time, i.e., most of light will be vertical
First incidence zone of first plane of incidence 201 enters the first prism 200.Due to being coated with reflectance coating, while on the first reflecting surface 203
One reflecting surface 203 and the first incidence zone are arranged at 45 degree of angles, and incident ray, will be by first when direct projection is to the first reflecting surface 203
Reflecting surface 203 changes direction of transfer, and the direction parallel with first plane of incidence 201 is transmitted to the second reflecting surface 204 by incident ray,
And changing the direction of transfer of light again by the second reflecting surface 204, the light after changing is perpendicular to the first exit facet 202 from
One outgoing area enters in lens barrel 210, since the central area of lens barrel 210 is arranged in the first outgoing area, into inciting somebody to action for lens barrel 210
The light for transmitting along lens barrel 210 along lens barrel central area, and finally emitting LED chip 101 or LED chip group 102 emits
Onto the object for needing to irradiate.The LED chip 101 or LED core of multiple and different central wavelength wave bands are set on the chassis LED 100
The LED chip group 102 that piece group 102 also may include LED chip 101 and be made of LED chip 101, when needing which wave band,
The LED chip 101 or LED chip group 102 and the first incidence zone are matched, it is unwanted without removing
LED chip 101 re-assemblies the LED chip 101 of needs again, realizes automatic replacement, uses and carries and is all very convenient.Together
When, light is changed direction of transfer realizing by the first prism 200, and except the light incident direction for reaching needs, light is
Pass through multiple reflections inside one prism 200, every secondary reflection can all form virtual light source picture, and multiple reflections form two-dimensional virtual optical
Source matrix achievees the effect that even light to keep output light more uniform.
Preferably, as shown in Figure 5, Figure 6, optical path subsystem further includes lens group, and lens group is arranged in lens barrel 210, thoroughly
Microscope group includes at least the first convex lens 510 and the second convex lens 520, and the first convex lens 510 is arranged in lens barrel 210 close to the first rib
200 end of mirror, and be set as the first outgoing area and be located in the focal length of the first convex lens 510；Second convex lens 520 is set as in lens barrel
210 far from 200 end of the first prism.Wherein, the second convex lens 520 includes convex-concave eyeglass 522 and planoconvex lens 521, convex-concave eyeglass
522 are arranged between planoconvex lens 521 and the first convex lens 510.Since the first outgoing area is located at the focal length of the first convex lens 510
Interior, the light emitted from the first outgoing area will realize the disperse function to light, then lead under the action of the first convex lens 510
The parallel rays that the light through dissipating is again transformed into collimation by the second convex lens 520 is crossed, the irradiation model of output light can be increased
It encloses.Lens adjusting device can be respectively set on the first convex lens 510 and the second convex lens 520, lens adjusting device can be with
Realize that the first convex lens 510 and the second convex lens 520 along the longitudinal back-and-forth motion of lens barrel 210, realize the first convex lens 510 and first
It is emitted the adjustment of distance between the adjustment and the second convex lens 520 and the first convex lens 510 of distance between area.Can also only it exist
Lens adjusting device is set on the second convex lens 520, and the distance between the first convex lens 510 and the first outgoing area are according to micro-
The performance of mirror presets an optimal fixed value.
Second prism 220 and the first prism 200 can be in line setting, i.e., using the center of circle of lens barrel 210 as reference point, first
Prism 200 and the second prism 220 are respectively along the diameter setting of a lens barrel 210, i.e., as shown in Figure 6.Second prism 220 and first
Prism 200 can also be at an angle of setting, i.e. the first prism 200 and the second prism 220 are set along lens barrel 210 radius respectively
It sets, the first prism 200 and the second prism 220 can be set in one plane, can also be separately positioned on the cross with lens barrel 210
In two parallel planes of section.This can be realized by only needing the projection of the first prism 200 and the second prism 220 not to be overlapped
The effect of utility model embodiment technical solution.Preferably, the first prism 200 and the setting of the second prism 220 are horizontal with lens barrel 210
In the parallel approximately the same plane in section, prism fixed plate 230 is set, and the first prism 200 and the second prism 220 are arranged at prism
In fixed plate 230.And prism centers micromatic setting 240 is set in prism fixed plate 230.And pass through prism centers micromatic setting
240, straight-line displacement adjustment of the prism fixed plate 230 in 210 cross-wise direction of lens barrel may be implemented, so that the LED of selected wave band
Chip 101 or LED chip group 102 are realized with the first incidence zone or the second incidence zone more accurately to be matched.By the first prism
200, the second prism 220 is arranged in the same prism fixed plate 230 simultaneously, can be convenient the first convex lens 510 to the first rib
Suitable distance is arranged in mirror 200 and the second prism 220 simultaneously, realizes the light issued simultaneously to the first outgoing area and the second outgoing area
Line is adjusted.
Wherein, the utility model embodiment provides two kinds of concrete implementation modes to control subsystem to realize LED core
The matching of piece 101 or LED chip group 102 and the first incidence zone or the second incidence zone.First way as shown in Figure 1
In, control subsystem includes first motor 210, and the first driving wheel 320 is set as hollow the first follower 330, first rotation volume
Code device 340 and control circuit (not shown), first motor 310 connect the shaft of the first driving wheel 320, the first follower 330 with
Prism fixed plate 230 is fixedly connected, and is assemblied on bearing block 420, and the first driving wheel 320 and the first follower 330 pass through biography
Dynamic band 350 connects, and the first rotary encoder 340 is connect with the shaft of the first driving wheel 320, the first rotary encoder 340 and control
Circuit signal connection processed, control circuit control LED chip 101 and/or 102 power circuit of LED chip group and 310 electricity of first motor
Source circuit.Wherein, in specific embodiment as shown in Figure 1, the first driving wheel 320, the first follower 330 are all belt pulley, the
One driving wheel 320 is realized by driving belt and is driven.First driving wheel 320, the first follower 330 can also use gear, the
Pass through chain drive between one driving wheel 320 and the first follower 330.The preferred belt transmission of the utility model embodiment, wherein
First follower 330 is set as hollow, can be convenient light and passes through without will cause light barrier.First follower 330 can be with
Radius is set as greater than the first driving wheel 320, the radius that the first follower 330 is such as arranged is the two of 320 radius of the first driving wheel
Times.When being set as 330 radius of the first follower is twice of 320 radius of the first driving wheel, the first driving wheel 320 rotates 2 degree,
First follower 330 will rotate 1 degree, may be implemented to adjust the more accurate angle of prism fixed plate 230, realize selected wave band
LED chip 101 or LED chip group 102 are more accurately matched with the first incidence zone, the second incidence zone.First motor 310 is preferred
Stepper motor.
Above-mentioned control subsystem realizes LED chip 101 or LED chip group 102 and the first incidence zone, the of selected wave band
Two incidence zones more accurately match, and specific control flow is as shown in fig. 7, it controls working principle are as follows:
The exciting light wave band according to required for the sample that microscope needs to observe first, selects the LED core with the wave band
Piece 101 inputs the coding C1 of the LED chip 101 or LED chip group 102 in control circuit.Control circuit will be opened and be walked
Into the power circuit of motor, and read the rotary encoder position on the first rotary encoder 340 being arranged on stepper motor
C2 is encoded, which has corresponded to the position of prism fixed plate 230, when C2 is matched with C1, such as C2=C1, the first prism at this time
Second incidence zone face of 200 the first incidence zone or the second prism 220 is encoded to the LED chip 101 or LED chip of C1
Group 102, control circuit judge that the LED chip 101 of selected wave band is realized and matched with the first prism 200 or the second prism 220.
The LED chip 101 for starting corresponding wave band or LED chip group 102 are connected driving power circuit by control circuit, make the LED core
Piece 101 or LED chip group 102 issue the wave band exciting light of selected wavelength.If C2 and C1 is mismatched, as C2 is not equal to C1
When, stepper motor is initially powered up rotation, and drives the rotation of the first follower 330 by the first driving wheel 320, to drive prism
Fixed plate 230 rotates.The position encoded C2 of the first rotary encoder of control circuit real-time detection 340, and in real time by the coding with
The LED chip 101 of selected wavelength encodes C1 and compares, when the position encoded C2 and chip that detect the first rotary encoder 340 are compiled
When code C1 matching, control stepper motor stops working.Start the LED chip 101 or LED chip group 102 of corresponding wave band simultaneously
Driving power circuit is connected, the LED chip 101 or LED chip group 102 is made to issue the wave band exciting light of selected wavelength.
In second of specific embodiment of control subsystem, control subsystem includes the second motor, the second driving wheel, the
Two followers, the second rotary encoder and control circuit, the second motor connect the shaft of the second driving wheel, and the second follower turns
The axle center on the chassis axis connection LED 100, the second driving wheel and the second follower by transmission band connection, the second rotary encoder with
The shaft of second driving wheel connects, and the second rotary encoder connect with control circuit signal, control circuit control LED chip with/
Or LED chip group power circuit and the second motor power circuit.
Its working principle of second of specific embodiment of control subsystem is similar with the first embodiment, difference
It is in in the first control mode, 100 position of the chassis LED immobilizes, by rotating prism fixed plate 230, to realize
The LED chip 101 for matching selected wavelength is actively gone in the matching of prism and LED chip 101 or LED chip group 102 by prism
Or LED chip group 102；And in second of embodiment, the position of prism immobilizes, by rotating LED chassis 100, from
And it realizes the LED chip 101 of selected wavelength or LED chip group 102 and goes active rotation incident to the first incidence zone or second
Qu Zhong.The first motor 310 of above two mode, the preferred stepper motor of the second motor, to preferably realize rotation angle
Accurate control.Control subsystem can also be mainly to realize the LED chip of selected wave band using other implementations, purpose
101 or the matched effect of LED chip group 102 and the first incidence zone or the second incidence zone.
Above embodiment is only preferred embodiments of the present invention, cannot be protected with this to limit the utility model
Range, the variation of any unsubstantiality that those skilled in the art is done on the basis of the utility model and replacement belong to
In the utility model range claimed.
Claims (10)
1. multiband LED fluorescence microscope, which is characterized in that described more including microscope body and multiband LED light source system
Wave band LED light source system is connect by connector sleeve with the fluorescent light source interface of the microscope ontology；
The multiband LED light source system includes the chassis LED, N number of LED chip and/or LED chip group；On the chassis LED
First circumference is set, and N number of LED chip and/or LED chip group interval are laid on first circumference；Wherein, the N
A LED chip and/or LED chip group include the wave band of n different central wavelengths, N >=n >=2；Multiband LED light source system
System further includes optical path subsystem, and the optical path subsystem includes the first prism and lens barrel, and first prism includes parallel the
One plane of incidence and the first exit facet, parallel the first reflecting surface and the second reflecting surface, first reflecting surface and the second reflecting surface
It is equipped with reflectance coating, first plane of incidence and the first reflecting surface are arranged at 45 degree of angles, first exit facet and the second reflection
Face is arranged at 45 degree of angles；First plane of incidence includes the first incidence zone, and first incidence zone is set as first reflection
View field of the face in first plane of incidence；First exit facet includes the first outgoing area, first outgoing area's setting
It is second reflecting surface in the view field of first exit facet, described lens barrel one end is arranged in first outgoing area
Central area；The multiband LED light source system further includes control subsystem, and the control subsystem is for controlling selected standing wave
First incidence zone described in long LED chip and/or LED chip group face.
2. multiband LED fluorescence microscope as described in claim 1, which is characterized in that the optical path subsystem further includes
Microscope group, the lens group are arranged in the lens barrel, and the lens group includes at least the first convex lens and the second convex lens, described
First convex lens is arranged in the lens barrel close to first prism end, and is set as first outgoing area and is located at described first
In the focal length of convex lens；Second convex lens is set as in the lens barrel far from first prism end, second convex lens
Mirror is arranged on lens adjusting device, and the lens adjusting device can adjust the second convex lens and be moved forward and backward along the lens barrel.
3. multiband LED fluorescence microscope as claimed in claim 2, which is characterized in that the setting and the on the chassis LED
Second circumference of one circumferential concentric, the LED chip and/or LED chip group interval are laid in first circumference and the second circle
Zhou Shang；
The optical path subsystem include the second prism, second prism include parallel second plane of incidence and the second exit facet,
Parallel third reflecting surface and the 4th reflecting surface, the third reflecting surface and the 4th reflecting surface be equipped with reflectance coating, described second
The plane of incidence and third reflecting surface are arranged at 45 degree of angles, and second exit facet and the 4th reflecting surface are arranged at 45 degree of angles；Described
Two planes of incidence include the second incidence zone, and second incidence zone is set as the third reflecting surface in the throwing of second plane of incidence
Shadow zone domain；Second exit facet includes the second outgoing area, and second outgoing area is set as the 4th reflecting surface described
The central area of described lens barrel one end is arranged in the view field of second exit facet, second outgoing area；
The control subsystem is used to control the LED chip and/or LED chip group face for being located at set wavelength on the first circumference
First incidence zone, or control are located on the second circumference described in the LED chip and/or LED chip group face of set wavelength
Second incidence zone.
4. multiband LED fluorescence microscope as described in claim 1, which is characterized in that the LED chip and/or LED chip
The wave band of the central wavelength of each of group LED chip or LED chip group is different, the LED chip and/or LED chip
The angular separation such as group are arranged on first circumference, and the center of circle of first circumference is arranged at the center on the chassis LED.
5. multiband LED fluorescence microscope as claimed in claim 3, which is characterized in that first prism and the second prism
It is rhombic prism.
6. the multiband LED fluorescence microscope as described in claim 3 to 5 is any, which is characterized in that the optical path subsystem is also
Including prism fixed plate, first prism and the second prism are fixed in the prism fixed plate, the prism fixed plate with
The lens barrel connection；The control subsystem includes first motor, and the first driving wheel is set as hollow the first follower, first
Rotary encoder and control circuit, the first motor connect the shaft of the first driving wheel, first follower and the rib
Mirror fixed plate is fixedly connected, and by transmission band connection, first rotation is compiled for first driving wheel and first follower
Code device is connect with the shaft of first driving wheel, and first rotary encoder is connect with the control circuit signal, described
Control circuit controls the LED chip and/or LED chip group power circuit and the first motor power circuit.
7. multiband LED fluorescence microscope as claimed in claim 1 to 5, which is characterized in that the control subsystem packet
Include the second motor, the second driving wheel, the second follower, the second rotary encoder and control circuit, the second motor connection the
The shaft of two driving wheels, the shaft of second follower connect the axle center on the chassis LED, second driving wheel with it is described
By transmission band connection, second rotary encoder connect second follower with the shaft of second driving wheel, and described the
Two rotary encoders are connect with the control circuit signal, and the control circuit controls the LED chip and/or LED chip group
Power circuit and second motor power circuit.
8. multiband LED fluorescence microscope as claimed in claim 6, which is characterized in that it further include prism centers micromatic setting,
The prism centers micromatic setting is connect with the prism fixed plate.
9. multiband LED fluorescence microscope as claimed in claim 5, which is characterized in that six faces of the rhombic prism are equal
Be set as burnishing surface, and the rhombic prism except the first incidence zone, the first outgoing area, the second incidence zone, the second outgoing area it
Reflective membrane is coated on outer all faces.
10. multiband LED fluorescence microscope as claimed in claim 2, which is characterized in that second convex lens includes convex-concave
Eyeglass and planoconvex lens, the convex-concave eyeglass are arranged between the planoconvex lens and first convex lens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821111475.8U CN208607422U (en) | 2018-07-13 | 2018-07-13 | Multiband LED fluorescence microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821111475.8U CN208607422U (en) | 2018-07-13 | 2018-07-13 | Multiband LED fluorescence microscope |
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| Publication Number | Publication Date |
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| CN208607422U true CN208607422U (en) | 2019-03-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201821111475.8U Active CN208607422U (en) | 2018-07-13 | 2018-07-13 | Multiband LED fluorescence microscope |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108761756A (en) * | 2018-07-13 | 2018-11-06 | 广州市明美光电技术有限公司 | Multiband LED fluorescence microscopes |
| CN108761756B (en) * | 2018-07-13 | 2024-06-28 | 广州市明美光电技术有限公司 | Multiband LED fluorescent microscope |
-
2018
- 2018-07-13 CN CN201821111475.8U patent/CN208607422U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108761756A (en) * | 2018-07-13 | 2018-11-06 | 广州市明美光电技术有限公司 | Multiband LED fluorescence microscopes |
| CN108761756B (en) * | 2018-07-13 | 2024-06-28 | 广州市明美光电技术有限公司 | Multiband LED fluorescent microscope |
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