CN208568803U - A kind of glycated albumin detection kit - Google Patents
A kind of glycated albumin detection kit Download PDFInfo
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- CN208568803U CN208568803U CN201821043129.0U CN201821043129U CN208568803U CN 208568803 U CN208568803 U CN 208568803U CN 201821043129 U CN201821043129 U CN 201821043129U CN 208568803 U CN208568803 U CN 208568803U
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Abstract
The utility model discloses a kind of glycated albumin detection kits, including PVC bottom plate, sample pad, fluorescence bonding pad, nitrocellulose filter, the first detection line, the second detection line, C line nature controlling line, water absorption pad.The utility model combination dry type immuno-fluorescence assay people's glycated albumin accounts for sero-abluminous ratio, by being directed to glycated albumin glycosylation site, and the specific monoclonal antibody in the non-glycosylated site of seralbumin, respectively with glycated albumin, seralbumin forms double-antibody sandwich structure, with good accuracy and specificity, GA is detected using immunization and by nanometer fluorescent microspheres simultaneously, it is convenient rapid, sensitivity precision is high, it is not influenced by the disturbing factors such as red blood cell life span and hemoglobin construct, it can be used for diabetic and monitor 2-3 weeks the past average blood glucose levels, especially gestational diabetes mellitus, diabetic nephropathy, hepatogenous peptic ulcer, it can also be used for screening for diabetes.
Description
Technical field
The utility model relates to medical product field more particularly to a kind of glycated albumin detection kits.
Background technique
Serum contains the protein there are many different type and function, and Tot Prot is about 60-80mg in every milliliter of serum.Blood
All protein in albumin, that is, serum, albumin are that wherein most important ingredient, all albumen have generation saccharification adduction
The possibility of reaction.After all serum protein components are reacted with carbohydrate generation saccharification, detectable a variety of glycated protein products
Mixture becomes fructosamine (FRUC), and wherein glycosylated albumin (GA) content is the abundantest.Glycosylated albumin is saccharification serum egg
Most important ingredient in white refers in particular to albumin with glucose and the product that nonenzymatic glycosylation reacts occurs.For diabetes clinic
The saccharification egg of diagnosis, treatment and curative effect monitoring is there are mainly two types of: glycosylated hemoglobin (GHB) and glycated serum protein (GSP).
Glycosylated hemoglobin is the product of glucose and hemoglobin saccharification reaction；Glycated serum protein is glucose and various serum eggs
The product of white sugarization reaction, due to the complexity of its ingredient, specificity is poor.The process of protein glycation is a kind of slowly non-
Enzymatic reaction, the albumen free amino being saccharified are also referred to as glycosylation site, and albumin molecule space structure is unique, is exposed
Glycosylation site enriches and wide variety, and because the site that saccharification occurs is different, glycosylated albumin production quantity is depended primarily in serum
The concentration of glucose and its time of contact with albumin, after albumin saccharification, the catabolism to the albumin to react
Process influence is unobvious, therefore GA half-life period is similar with ALB, and concentration level can reflect the average blood sugar of measurement first 20 days or so
It is horizontal.
There are many kinds of the measuring methods of GA, establishes thiobarbituric acid colorimetric successively the 1970s to the nineties
(TBA) method, ion exchange chromatography, affinity chromatography, immunoassay, enzymoimmunoassay, enzyme linked immunological borate method.
What clinical application and laboratory quantitative determined is GA component or " being equivalent to GA " component, i.e., the ratio of total serum albumin is accounted for using GA
Example (%) indicates that the concentration of GA, this kind of detection method are conducive to monitoring 2-3 weeks diabetic's the past average blood glucose levels, especially
It is gestational diabetes mellitus, diabetic nephropathy, hepatogenous peptic ulcer, it can also be used to screening for diabetes.
Therefore, there are also to be developed for the prior art.
Utility model content
Place in view of above-mentioned deficiencies of the prior art, the purpose of this utility model is to provide a kind of glycated albumins
Detection kit, it is intended to realize the purpose of the monitoring to 2-3 weeks diabetic's the past average blood glucose levels.
In order to achieve the above object, the utility model takes following technical scheme:
A kind of glycated albumin detection kit, including PVC bottom plate, sample pad, fluorescence bonding pad, nitrocellulose
Film, the first detection line, the second detection line, C line nature controlling line, water absorption pad, PVC bottom plate upper surface are from left to right successively arranged sample
Product pad, fluorescence bonding pad, nitrocellulose filter and water absorption pad, the nitrocellulose filter are fixed on the centre of the PVC bottom plate,
Described water absorption pad one end is fixed on PVC bottom plate right end, and the water absorption pad other end is fixed at nitrocellulose filter right end 2mm,
Fluorescence bonding pad one end is fixed at the left end 2mm of the nitrocellulose filter, and the other end of the fluorescence bonding pad is solid
It is scheduled on the PVC bottom plate, described sample pad one end is fixed at the other end 2mm of the fluorescence bonding pad, the sample pad
The other end is fixed on the left end of the PVC bottom plate；
The fluorescence bonding pad is equipped with coating hs-CRP antibody-nanometer fluorescent microspheres compound；
The nitrocellulose filter be equipped with coating the anti-human glycated albumin monoclonal antibody of mouse the first detection line,
It is coated with the second detection line and C line nature controlling line of mouse anti-human serum albumin monoclonal antibody II.
Further, the nanometer fluorescent microspheres are the red fluorescent microsphere of aldehyde radicalization, the nanometer of the nanometer fluorescent microspheres
Grain partial size is 100-200nm.
Further, the test sample of the glycated albumin detection kit is serum, blood plasma or whole blood.
Further, the fluorescence bonding pad material is polyester fiber.
The utility model compared with prior art the utility model has the advantages that the utility model combination dry type immuno-fluorescence assay people
Glycated albumin accounts for the ratio of total serum albumin, by being directed to glycated albumin glycosylation site and serum
The specific monoclonal antibody in the non-glycosylated site of albumin forms double antibody folder with glycated albumin, total serum albumin respectively
Core structure has good accuracy and specificity, while detecting GA using immunization and by nanometer fluorescent microspheres, convenient fast
Speed, sensitivity precision is high, is not influenced by the disturbing factors such as red blood cell life span and hemoglobin construct, can be used for diabetic
Monitor 2-3 weeks the past average blood glucose levels, especially gestational diabetes mellitus, diabetic nephropathy, hepatogenous peptic ulcer, it can also be used to sugar
Urinate sick screening.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of glycated albumin detection kit provided by the embodiment of the utility model.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element
On one element or it may be simultaneously present centering elements.When an element is known as " being connected to " another element, it can
To be directly to another element or may be simultaneously present centering elements.
It is only each other relatively it should also be noted that, the positional terms such as left and right, upper and lower in the utility model embodiment
Concept or be reference with the normal operating condition of product, and should not be regarded as restrictive.
As shown in Figure 1, being a kind of glycated albumin detection kit of the utility model specific embodiment
Including PVC bottom plate 1, sample pad 2, fluorescence bonding pad 3, nitrocellulose filter 5, the first detection line 6, the second detection line
7, C line nature controlling line 8, water absorption pad 4,1 upper surface of PVC bottom plate are from left to right successively arranged sample pad 2, fluorescence bonding pad 3, nitre
Acid cellulose film 5 and water absorption pad 4, the nitrocellulose filter 5 are fixed on the centre of the PVC bottom plate 1, the water absorption pad 4 one
End is fixed on 1 right end of PVC bottom plate, and 4 other end of water absorption pad is fixed at 5 right end 2mm of nitrocellulose filter, the fluorescence knot
It closes 3 one end of pad to be fixed at the left end 2mm of the nitrocellulose filter 5, the other end of the fluorescence bonding pad 3 is fixed on described
On PVC bottom plate 1, described 2 one end of sample pad is fixed at the other end 2mm of the fluorescence bonding pad 3, and the sample pad 2 is another
End is fixed on the left end of the PVC bottom plate 1；
The fluorescence bonding pad 3 is equipped with coating hs-CRP antibody-nanometer fluorescent microspheres compound；
The nitrocellulose filter 5 is equipped with the first detection line of the coating anti-human glycated albumin monoclonal antibody of mouse
6, the second detection line 7 and C line nature controlling line 8 of mouse anti-human serum albumin monoclonal antibody II are coated with.
Specifically, the nanometer fluorescent microspheres are the red fluorescent microsphere of aldehyde radicalization, the nano particle of the nanometer fluorescent microspheres
Partial size is 100~200nm.
Wherein, the excitation wavelength of the nanometer fluorescent microspheres is 365nm, and launch wavelength is 607~617nm, surface aldehydes
Group content is 0.1~0.5mmol/g.
Specifically, the test sample of the glycated albumin detection kit is serum, blood plasma or whole blood.
Specifically, 3 material of fluorescence bonding pad is polyester fiber.
In the present invention, 4 fixing end of water absorption pad is right end, and 2 fixing end of sample pad is left end.
The manufacturing process of specific detection kit is as follows:
Using nanometer fluorescent microspheres particle marker mouse anti-human serum albumin antibody I, save backup；
Nitrocellulose filter-PVC bottom plate: nitrocellulose filter 5 is cut into the slice of wide 25mm long 300mm, is attached to the bottom PVC
On plate 1, the anti-human glycated albumin of mouse and mouse anti-human serum albumin monoclonal antibody II are drawn on nitrocellulose filter
Line obtains two detection lines, i.e. the first detection line 6, the second detection line 7 cross rabbit anti-mouse igg on nitrocellulose filter
To nature controlling line, it is dry that nitrocellulose filter-PVC bottom plate is then placed in drying box；
Sample pad 2: glass fibre membrane is cut into the slice of wide 17mm long 300mm；
Absorption pad 7: blotting paper is cut into the slice of wide 17mm long 300mm；
Fluorescence bonding pad: being cut into the slice of wide 10mm long 300mm by fluorescence bonding pad 3, by mouse anti-human serum albumin
Monoclonal antibody I nanometer fluorescent microspheres compound, dry, the fluorescence bonding pad prepared.
By the sample pad 2 prepared, fluorescence bonding pad 3, successively nitrocellulose filter 5 is pasted in PVC bottom plate 1 close to
One detection line, 6 left end, and guarantee that fluorescence bonding pad 3 covers nitrocellulose filter 2mm, sample pad 2 covers fluorescence bonding pad 2mm,
Water absorption pad 4 pastes in PVC bottom plate 1 nitrocellulose filter 5 close to C line nature controlling line right end, and guarantees that water absorption pad 4 covers nitric acid fibre
Plain film 2mm is tieed up, the test strips that cutting machine is cut into one fixed width are reused.
One of the utility model glycated albumin detection kit in use, with pipettor take 60ul serum,
Plasma sample or 100ul whole blood sample are added on the sample-adding pad of test strips, stand the chromatography of waiting in 10 minutes at room temperature, and chromatography terminates
Afterwards, test strips are put into immunofluorescence quantitative analysis instrument and read data, immunofluorescence quantitative analysis instrument carries out fluorescence signal
Measurement and analysis processing obtain the fluorescence signal intensity of C line, the first detection line and the second detection line, and automatically according to standard song
The parameter of line setting obtains tested sample concentration.
The utility model detects hs-CRP using competition law reaction principle combination nanometer fluorescent microspheres, diagnosis
Some protein steeply risen in blood plasma compare general clinical symptoms integral diagnosis method, as a result relatively reliable science, are
Body is infected or the diagnosis of tissue damage provides index and method definitely, and the nano fluorescent of this practical use
Microspheres Technique, technology detect background value it is low, detection time is short, specific and sensitivity is higher, testing result can quantum chemical method,
It can provide more scientific state of an illness evaluation.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model
Protection scope within.
Claims (4)
1. a kind of glycated albumin detection kit, including PVC bottom plate, sample pad, fluorescence bonding pad, nitrocellulose
Film, the first detection line, the second detection line, C line nature controlling line, water absorption pad, it is characterised in that: PVC bottom plate upper surface from a left side to
The right side is successively arranged sample pad, fluorescence bonding pad, nitrocellulose filter and water absorption pad, and the nitrocellulose filter is fixed on described
The centre of PVC bottom plate, described water absorption pad one end are fixed on PVC bottom plate right end, and the water absorption pad other end is fixed on cellulose nitrate
At plain film right end 2mm, fluorescence bonding pad one end is fixed at the left end 2mm of the nitrocellulose filter, the fluorescence knot
The other end for closing pad is fixed on the PVC bottom plate, and described sample pad one end is fixed on the other end 2mm of the fluorescence bonding pad
Place, the sample pad other end are fixed on the left end of the PVC bottom plate；
The fluorescence bonding pad is equipped with coating hs-CRP antibody-nanometer fluorescent microspheres compound；
The nitrocellulose filter is equipped with the first detection line of the coating anti-human glycated albumin monoclonal antibody of mouse, coating
The second detection line and C line nature controlling line of mouse anti-human serum albumin monoclonal antibody II.
2. glycated albumin detection kit according to claim 1, it is characterised in that: the nanometer fluorescent microspheres
For the red fluorescent microsphere of aldehyde radicalization, the nano particle diameter of the nanometer fluorescent microspheres is 100-200nm.
3. glycated albumin detection kit according to claim 2, it is characterised in that: the white egg of the saccharification serum
The test sample of white detection kit is serum, blood plasma or whole blood.
4. glycated albumin detection kit according to claim 1, it is characterised in that: the fluorescence combination mat material
Matter is polyester fiber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821043129.0U CN208568803U (en) | 2018-07-03 | 2018-07-03 | A kind of glycated albumin detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821043129.0U CN208568803U (en) | 2018-07-03 | 2018-07-03 | A kind of glycated albumin detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN208568803U true CN208568803U (en) | 2019-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821043129.0U Active CN208568803U (en) | 2018-07-03 | 2018-07-03 | A kind of glycated albumin detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208568803U (en) |
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2018
- 2018-07-03 CN CN201821043129.0U patent/CN208568803U/en active Active
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