CN208532657U

CN208532657U - Polypeptide laser printer

Info

Publication number: CN208532657U
Application number: CN201820949172.7U
Authority: CN
Inventors: 魏末初
Original assignee: Wuhan Science And Technology Co Ltd
Current assignee: Wuhan Science And Technology Co Ltd
Priority date: 2018-06-19
Filing date: 2018-06-19
Publication date: 2019-02-22
Anticipated expiration: 2028-06-19

Abstract

The utility model provides a kind of polypeptide laser printer, it is related to bio-pharmaceutical synthesis device technical field, including the storage box, printing equipment, shell, print pedestal and mobile device, the storage box passes through piping connection printing equipment, the storage box and printing pedestal are fixedly connected in shell, mobile device connection printing pedestal, printing equipment connects mobile device, it is low to alleviate Peptide systhesis the degree of automation existing in the prior art, aggregate velocity is slow, the low technical problem of accuracy class, polypeptide is realized automatically to synthesize, aggregate velocity is fast, and it is easy to improve the technical effect of the technical effect of synthesis precision.

Description

Polypeptide laser printer

Technical field

The utility model relates to bio-pharmaceutical synthesis device technical fields, more particularly, to a kind of polypeptide laser printer.

Background technique

Peptide (peptide) is a-amino acid with the compound that peptide chain links together and is formed, it is also protein hydrolysis Intermediate product dipeptides, tripeptides can be described as according to the difference of the quantity of amino acid in peptide, until nonapeptide.Polypeptide is that human body is inhaled The principal mode of protein is received, has and is actively absorbed by the body, and polypeptide body will not be destroyed, does not increase human body energy consumption, plays The effect of good nutrition carrier and means of transport, therefore polypeptide has effect of crucial importance for human health, thus polypeptide Synthesis causes the great attention of various countries' life scientist.

Peptide systhesis is the process that amino acid is added in a repetition, and synthesis in solid state sequence is generally from the end C (c-terminus) to N Hold (aminoterminal) synthesis.Solid-phase synthesis greatly alleviates the difficulty of every step product purification.The hair of side reaction in order to prevent Raw, the side chain for participating in the amino acid of reaction is all protection.C-terminus is free, and must be activated before reactions.Gu It is combined to there are two types of methods, i.e. Fmoc and tBoc.Since there are many advantages by Fmoc ratio tBoc, Fmoc method is mostly used to close greatly now At.

First generation Peptide synthesizer is the 990 Peptide Synthesizer of Beckman released with Beckman company And the 296 Peptide Synthesizer of Vega ' s of Vega ' s Biotechnologies company release is the conjunction of representative Cheng Yi, with the improvement and development of production technology, nowadays first generation Peptide synthesizer has all exited market.

Domestic common Peptide systhesis is divided into hand held device synthesis at present, and semi-automated instrument synthesis and full-automatic instrument close At, hand held device synthesis or semi-automated instrument synthesis are mostly used greatly, full-automatic instrument synthesis is still in starting developing stage, for Ultra-high throughput synthesis is even more to become worldwide problem.

Utility model content

In view of this, the purpose of this utility model is to provide a kind of polypeptide laser printer, to alleviate in the prior art Existing Peptide systhesis the degree of automation is low, and aggregate velocity is slow, the low technical problem of accuracy class.

In a first aspect, the utility model embodiment provides a kind of polypeptide laser printer, the storage box, is beaten printing equipment Pedestal and mobile device are printed, the storage box is connected by flexible conduit and the printing equipment flow communication, the mobile device It connects at the edge of the printing pedestal, the printing equipment connects the mobile device, and the printing equipment is in vertical direction Projection is located on the printing pedestal.

With reference to first aspect, the utility model embodiment provides the first possible embodiment of first aspect, In, the quantity of the storage box is more than or equal to 24.

The possible embodiment of with reference to first aspect the first, the utility model embodiment provide the of first aspect Two kinds of possible embodiments, wherein the printing equipment includes jet nozzle, spray head, feed pipe and printed mechanism, the spray head It is equal with the quantity of the storage box with the quantity of the feed pipe, multiple spray heads correspondingly with it is multiple it is described into Expects pipe is connected to and in communication with, and multiple spray heads are all connected to the jet nozzle, and multiple spray heads are in a word along the jet nozzle It is uniformly distributed, multiple the storage box are connected to by the flexible conduit correspondingly with multiple feed pipes, the print Mechanism processed is to make amino acid enter the spray head from the feed pipe.

The possible embodiment of second with reference to first aspect, the utility model embodiment provide the of first aspect Three kinds of possible embodiments, wherein the printed mechanism is laser emitter.

The possible embodiment of second with reference to first aspect, the utility model embodiment provide the of first aspect Four kinds of possible embodiments, wherein the printed mechanism includes liquid pump and solenoid valve, and the solenoid valve is arranged in feed pipe Interior, the liquid feeding end of the liquid pump connects the feed pipe, and the outlet end of the liquid pump connects the spray head.

With reference to first aspect, the utility model embodiment provides the 5th kind of possible embodiment of first aspect, In, being arranged side by side on the printing pedestal has sliding channel and sheet glass embedding groove, and the length of the sliding channel is not less than institute State the length of sheet glass embedding groove.

The 5th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect Six kinds of possible embodiments, wherein the mobile device includes the first translation mechanism and the second translation mechanism, and described first is flat Telephone-moving structure is slide the printing equipment along the direction vertical with the sliding channel length direction, the second translation machine Structure is slide the printing equipment along the length direction of the sliding channel.

The 6th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect Seven kinds of possible embodiments, wherein first translation mechanism include the first stepper motor, the first lead screw, stepper motor seat, Slide bar and sliding pin, first lead screw connect the drive shaft of first stepper motor, the first stepping electricity by shaft coupling Machine is fixedly connected with the stepper motor seat, and the slide bar and the sliding pin are all fixedly connected with the stepper motor seat, and described first The axis of lead screw is parallel with the axis of the slide bar, and first lead screw is bolted in the printing equipment, and the printing equipment is sliding Dynamic to be socketed on the slide bar, the sliding pin is snug fit in the sliding channel.

The 7th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect Eight kinds of possible embodiments, wherein second translation mechanism includes second stepper motor and the second lead screw, the second step It is fixedly connected on the printing pedestal into motor, second lead screw connects the transmission of the second stepper motor by shaft coupling Axis, second lead screw are bolted in the stepper motor seat, and the axis of second lead screw is parallel to the length of the sliding channel Spend direction.

The 5th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect Nine kinds of possible embodiments, wherein finger pressure through slot and finger pressure wraping plate, the finger pressure wraping plate are additionally provided on the printing pedestal Middle-end be articulated with the printing pedestal, the finger pressure wraping plate is set in the finger pressure through slot, one end position of the finger pressure wraping plate In the bottom of the sheet glass embedding groove.

The utility model embodiment brings following the utility model has the advantages that storing amino acid, printing pedestal carrying using the storage box Glass-chip, the amino acid in the storage box enter printing equipment by pipeline, and printing equipment prints amino acid to glass-chip On, mobile device drives printing equipment mobile relative to glass-chip, to change printing position of the printing equipment on glass-chip It sets, specific amino acids is printed to specific position on glass-chip, compared with the relevant technologies, polypeptide provided by the utility model swashs Optical printer solves polypeptide in the prior art and at using manual or semi-automatic synthesis equipment, cannot achieve full-automatic synthesis The technical issues of, so that the printing of amino acid specific position on printing equipment is realized, to be automatically synthesized the technology of polypeptide Effect.

Other feature and advantage of the utility model will illustrate in the following description, also, partly from specification In become apparent, or understood and implementing the utility model.The purpose of this utility model and other advantages are illustrating Specifically noted structure is achieved and obtained in book and attached drawing.

To enable the above objects, features, and advantages of the utility model to be clearer and more comprehensible, preferred embodiment is cited below particularly, and Cooperate appended attached drawing, is described in detail below.

Detailed description of the invention

Technical solution in order to illustrate more clearly of specific embodiment of the present invention or in the related technology, below will be right Attached drawing needed in specific embodiment or description of Related Art is briefly described, it should be apparent that, it is described below In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art Under the premise of labour, it is also possible to obtain other drawings based on these drawings.

Fig. 1 is polypeptide laser printer structural schematic diagram one provided by the embodiment of the utility model；

Fig. 2 is polypeptide laser printer structural schematic diagram two provided by the embodiment of the utility model；

Fig. 3 is printing equipment structural schematic diagram provided by the embodiment of the utility model；

Fig. 4 is the first translation mechanism structural schematic diagram provided by the embodiment of the utility model；

Fig. 5 is printing base construction schematic diagram provided by the embodiment of the utility model.

Icon: 1- the storage box；2- printing equipment；21- jet nozzle；22- tapped through hole；23- slide bar through-hole；24- spray head； 25- feed pipe；26- printed mechanism；3- shell；4- prints pedestal；41- sliding channel；42- sheet glass embedding groove；43- finger pressure Through slot；44- finger pressure wraping plate；The first translation mechanism of 5-；The first stepper motor of 51-；The first lead screw of 52-；53- stepper motor seat；54- Slide bar；55- sliding pin；The second translation mechanism of 6-.

Specific embodiment

The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical Novel protected range.

It is in the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", " perpendicular Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition, term " the One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.

In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally Connection；It can be mechanical connection, be also possible to be electrically connected；Can be directly connected, can also indirectly connected through an intermediary, It can be the connection inside two elements.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition The concrete meaning of language in the present invention.

At present using manual or semi-automatic Peptide systhesis, polypeptide is low at the degree of automation, and aggregate velocity is slow, accuracy class It is low, it is based on this, polypeptide laser printer provided by the embodiment of the utility model can be realized automatically amino acid and beat in specific position Print compared to manually or semi-automatic synthesis, has high degree of automation being automatically synthesized polypeptide, be easy to improve synthesis precision and The advantages of efficiency.

Embodiment one

As shown in Figure 1, polypeptide laser printer provided by the embodiment of the utility model, comprising: the storage box 1, printing equipment 2, shell 3, printing pedestal 4 and mobile device, the storage box 1 connect printing equipment 2 by flexible conduit, are used for inside the storage box 1 Amino acid is stored, the amino acid inside the storage box 1 is delivered to printing equipment 2, the storage box 1 and printing pedestal 4 by flexible conduit It is fixedly connected in shell 3, shell 3 has fixed supporting role to the storage box 1 and printing pedestal 4, this outer housing 3 is adopted It is made of resistant to high temperatures and heat-barrier material, is conducive to keep the temperature the inside of shell 3, and then ensure to be suitable for inside shell 3 more Peptide synthesis, mobile device are divided into fixing end and mobile terminal, and mobile device connects printing pedestal 4 by fixing end, and printing equipment 2 connects The mobile terminal for connecing mobile device, the mobile terminal by controlling mobile device can make printing equipment 2 be moved to specific position, print Glass-chip is set above pedestal 4, is moved to the printing equipment 2 of specific position printing amino acid to the spy of glass-chip Positioning is set, consequently facilitating a variety of amino acid are arranged by certain positional relationship, and then realizes the full-automatic synthesis of polypeptide；

It should be noted that the amino acid of 1 storage inside of the storage box should have certain high temperature resistance, and should have bad Propylhomoserin protection, ensures that amino acid meets the conditions dictate of Peptide systhesis with this.

In one optional embodiment of the utility model embodiment, the quantity of the storage box 1 is more than or equal to 24, Duo Gecun Storage box 1 is respectively to store different types of amino acid, in addition to 20 kinds of amino acid of composition native protein, 20 kinds of basic ammonia The derivative of base acid may also be stored in the raw material in the storage box 1 as Peptide systhesis.

As shown in figure 3, printing equipment 2 includes jet nozzle in another optional embodiment of the utility model embodiment 21, spray head 24, feed pipe 25 and printed mechanism 26 are fixedly connected with multiple spray heads 24, spray head 24 and feed pipe 25 on jet nozzle 21 It is identical as the quantity of the storage box 1, multiple feed pipes 25, multiple feed pipes are connected to and in communication on multiple spray heads 24 correspondingly 25 are connected to multiple the storage box 1 by flexible conduit correspondingly, and when printing equipment 2 is mobile, flexible conduit is to adapt to The distance between printing equipment 2 and the storage box 1 variation, printed mechanism 26 to make amino acid from feed pipe 25 enter spray head 24, Multiple spray heads 24 are respectively to convey on different types of amino acid to glass-chip, so as to realize that a variety of amino acid are pressed Particular sequence arrangement, whether printed mechanism 26, which passes through spray head 24 for controlling amino acid, is discharged, to control the supply of amino acid Amount, printed mechanism 26 are equal to the amino acid output device of semi-automatic polypeptide synthesizer in the prior art.

Embodiment two

As shown in figure 3, the utility model embodiment is the further explanation to embodiment one, and in the present embodiment, print Mechanism 26 processed is laser emitter, and by high temperature resistant, and the solid-state amino acid after activation is placed in inside the storage box 1, laser emitter Generate high temperature can will it is amino acid converting be liquid, laser energy act under, liquid amino acid is attached on glass-chip, this Process will make amino acid rapid synthesis polypeptide under of short duration hot environment, use liquid amino acid synthesis polypeptide compared to tradition Technology has aggregate velocity fast, and easily controllable amino acid supply amount compares mg grades of synthetic technologys of tradition, can be by Peptide systhesis essence Degree is improved to ng grades even pg grades, makes it possible that the ultra-high throughput of polypeptide synthesizes；

It should be noted that the control of laser emitter and mobile device, need to lead to control chip programming and realize, control chip Program code can refer to polypeptide synthesis method in the prior art, in conjunction with required amino acid sequence, work to mobile device and The working condition of laser emitter carries out coordinated control, is the prior art in view of polypeptide synthesis method, therefore not right in this specification This does excessive elaboration.

Embodiment three

The utility model embodiment is the further explanation to embodiment one, and in the present embodiment, printed mechanism 26 is wrapped Liquid pump and solenoid valve are included, solenoid valve is arranged in feed pipe 25, and the liquid feeding end of liquid pump connects feed pipe 25, and the outlet end of liquid pump connects Spray head 24 is connect, in this scheme, it should be noted that, 1 storage inside of the storage box is liquid amino acid, and liquid amino acid is in liquid pump Under effect, and pass through solenoid valve control discharge rate, to realize the quantitative output of amino acid, the control of liquid pump and solenoid valve is Well-known technique, therefore do not repeat them here in the present specification.

Example IV

As shown in figure 5, the utility model embodiment is the further explanation to embodiment one, and in present embodiment, printing Being arranged side by side on pedestal 4 has sliding channel 41 and sheet glass embedding groove 42, for placing glass core inside sheet glass embedding groove 42 Piece realizes the positioning to glass-chip, glass-chip is for carrying and closing by 42 card column glass-chip of sheet glass embedding groove At polypeptide, the length of sliding channel 41 is not less than the length of sheet glass embedding groove 42, to make printing equipment 2 along sliding channel 41 When sliding, spray head 24 slides into any position of 42 length direction of sheet glass embedding groove therewith, so as to by amino acid transport extremely Any position of 42 inner glass chip of sheet glass embedding groove.

In addition, as shown in Fig. 2, mobile device includes the first translation mechanism 5 and the second translation mechanism 6, the first translation mechanism 5 Slide printing equipment 2 along the vertical direction of 41 length of sliding channel, the second translation mechanism 6 is to make 2 edge of printing equipment The length direction of sliding channel 41 slides, and printing equipment 2 can be made to move by the first translation mechanism 5 and the combination of the second translation mechanism 6 It moves to any position on glass-chip, and then can realize the synthesis of specific amino acids specific position on glass-chip.

As shown in figure 4, the first translation mechanism 5 includes the first stepper motor 51, the first lead screw 52, stepper motor seat 53, slides Bar 54 and sliding pin 55, the first lead screw 52 connect the drive shaft of the first stepper motor 51 by shaft coupling, to realize the first stepping Motor 51 drives the operating of the first lead screw 52, and the first stepper motor 51 is fixedly connected with stepper motor seat 53, and slide bar 54 and sliding pin 55 are all It is fixedly connected with stepper motor seat 53, the axis of the first lead screw 52 is parallel with the axis of slide bar 54, is provided with screw thread on printing equipment 2 Through-hole 22 and slide bar through-hole 23, the first lead screw 52 are bolted in tapped through hole 22, and slide bar through-hole 23 is slidably socketed in slide bar 54 On, when the first lead screw 52 rotates, the first lead screw 52 drives printing equipment 2 along the axis of the first lead screw 52 and the axis side of slide bar 54 To movement；Sliding pin 55 is slidably connected in sliding channel 41, and under the driving effect of the second translation mechanism 6, sliding pin 55 is along sliding Mobile achievable first translation mechanism 5 of through slot 41 is moved along the axis of the first lead screw 52 and the axis vertical direction of slide bar 54, into And printing equipment 2 is driven by the first translation mechanism 5, realize printing equipment 2 along the axis of the first lead screw 52 and the axis of slide bar 54 Line vertical direction is mobile.

It should be noted that the second translation mechanism 6 includes that second stepper motor and the second lead screw, second stepper motor are fixed It is connected to printing pedestal 4, the second lead screw connects the transmission shaft of second stepper motor by shaft coupling, and the second lead screw is bolted in stepping Motor cabinet 53, the axis of the second lead screw are parallel to the length direction of sliding channel 41, and the rotation of the second lead screw can drive the first translation Mechanism 5 is moved along the axis direction of the second lead screw, also, the sliding pin 55 slided inside sliding channel 41 limits the first translation The freedom degree that mechanism 5 is rotated around the second lead screw, to ensure that the first translation mechanism 5 along the sliding of sliding channel 41.

In the utility model embodiment, prints and be additionally provided with finger pressure through slot 43 and finger pressure wraping plate 44 on pedestal 4, finger pressure is stuck up The middle-end of plate 44 is articulated with printing pedestal 4, and finger pressure wraping plate 44 is set in finger pressure through slot 43, and one end of finger pressure wraping plate 44 is located at glass The bottom of glass piece embedding groove 42, when glass-chip is placed on sheet glass 42 inside of embedding groove, finger pressure wraping plate 44 is in a horizontal state, more After peptide synthesis, the other end by pressing finger pressure wraping plate 44 can make finger pressure wraping plate 44 be located at 42 bottom of sheet glass embedding groove One end tilt, so that glass-chip be made to tilt, with this convenient for operator by the glass-chip inside sheet glass embedding groove 42 It takes out, clamping stagnation after glass-chip expanded by heating is avoided to be not easily taken out in sheet glass embedding groove 42.

Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new The range of each embodiment technical solution of type.

Claims

1. a kind of polypeptide laser printer characterized by comprising the storage box (1), printing equipment (2), printing pedestal (4) and Mobile device, the storage box (1) pass through flexible conduit and the printing equipment (2) flow communication, the mobile device connection At the edge of printing pedestal (4), the printing equipment (2) connects the mobile device, and the printing equipment (2) is vertical The projection in direction is located on the printing pedestal (4).

2. polypeptide laser printer according to claim 1, which is characterized in that the quantity of the storage box (1) be greater than etc. In 24.

3. polypeptide laser printer according to claim 2, which is characterized in that the printing equipment (2) includes jet nozzle (21), the quantity of spray head (24), feed pipe (25) and printed mechanism (26), the spray head (24) and the feed pipe (25) with The quantity of the storage box (1) is equal, and multiple spray heads (24) connect simultaneously with multiple feed pipes (25) correspondingly Connection, multiple spray heads (24) are all connected to the jet nozzle (21), and multiple spray heads (24) are along the jet nozzle (21) It is uniformly distributed in a word, multiple the storage box (1) and multiple feed pipes (25) pass through the flexible pipe correspondingly Road connection, the printed mechanism (26) is to make amino acid enter the spray head (24) from the feed pipe (25).

4. polypeptide laser printer according to claim 3, which is characterized in that the printed mechanism (26) is Laser emission Device.

5. polypeptide laser printer according to claim 3, which is characterized in that the printed mechanism (26) include liquid pump and Solenoid valve, in feed pipe (25), the liquid feeding end of the liquid pump connects the feed pipe (25), the liquid for the solenoid valve setting The outlet end of pump connects the spray head (24).

6. polypeptide laser printer according to claim 1, which is characterized in that be arranged side by side on the printing pedestal (4) There are sliding channel (41) and sheet glass embedding groove (42), the length of the sliding channel (41) is more than or equal to the sheet glass and inlays The length of slot (42).

7. polypeptide laser printer according to claim 6, which is characterized in that the mobile device includes the first translation machine Structure (5) and the second translation mechanism (6), first translation mechanism (5) is to make the printing equipment (2) along logical with the sliding The vertical direction sliding of slot (41) length direction, second translation mechanism (6) is to make the printing equipment (2) along the cunning The length direction sliding of dynamic through slot (41).

8. polypeptide laser printer according to claim 7, which is characterized in that first translation mechanism (5) includes the One stepper motor (51), the first lead screw (52), stepper motor seat (53), slide bar (54) and sliding pin (55), first lead screw (52) drive shaft of first stepper motor (51) is connected by shaft coupling, first stepper motor (51) is fixedly connected on The stepper motor seat (53), the slide bar (54) and the sliding pin (55) are fixedly connected with the stepper motor seat (53), institute The axis for stating the first lead screw (52) is parallel with the axis of the slide bar (54), and first lead screw (52) is bolted in the printing dress It sets (2), the printing equipment (2) is slidably socketed in the slide bar (54), and it is logical that the sliding pin (55) is snug fit at the sliding In slot (41).

9. polypeptide laser printer according to claim 8, which is characterized in that second translation mechanism (6) includes the Two stepping motor and the second lead screw, the second stepper motor are fixedly connected on the printing pedestal (4), and second lead screw is logical The transmission shaft that shaft coupling connects the second stepper motor is crossed, second lead screw is bolted in the stepper motor seat (53), institute The axis for stating the second lead screw is parallel to the length direction of the sliding channel (41).

10. polypeptide laser printer according to claim 6, which is characterized in that be additionally provided on the printing pedestal (4) Finger pressure through slot (43) and finger pressure wraping plate (44), the middle-end of the finger pressure wraping plate (44) are articulated with the printing pedestal (4), the finger Wraping plate (44) are pressed to be set in the finger pressure through slot (43), one end of the finger pressure wraping plate (44) is located at the sheet glass embedding groove (42) bottom.