CN208485894U - Transfected by ultrasound mediated destruction of microbubbles device - Google Patents

Abstract

The utility model discloses a kind of transfected by ultrasound mediated destruction of microbubbles devices, including liquid storage container, kickboard, ultrasonic transducer and culture dish, and liquid storage container is to contain acoustic contrast agent, wherein kickboard is located in the liquid storage container and floats in the acoustic contrast agent；Ultrasonic transducer is installed in the kickboard, and is suspended in the acoustic contrast agent under the buoyancy of the kickboard；Culture dish is suspended at the top in the liquid storage container and being located at the ultrasonic transducer.According to transfected by ultrasound mediated destruction of microbubbles device provided by the embodiment of the utility model, ultrasonic transducer is suspended in acoustic contrast agent by kickboard, so, does not contact or fix with other fixing pieces, when ultrasonication is in acoustic contrast agent in liquid storage container, ultrasonic energy is passed directly in acoustic contrast agent, there is no energy loss, so that the amount that acoustic contrast agent generates microvesicle is bigger, and then transfection can be improved.

Description

Transfected by ultrasound mediated destruction of microbubbles device

Technical field

The utility model relates to medical equipment more particularly to a kind of transfected by ultrasound mediated destruction of microbubbles devices.

Background technique

With more recognizing human gene, the generation of many diseases is all related with gene, and gene therapy can be from root It is solved the problems, such as on this, becomes treatment tumour and the hope of other genetic diseases.The key of gene therapy is to mention high gene transfection Rate.

The tool for reprinting gene at present is mainly divided to two classes: one kind is viral vectors, and another kind of is non-viral carrier, such as Liposome, plasmid.The former can transfect gene to a variety of histocytes, and transfection efficiency is high, but virus itself has immunogenicity, Human body can be caused to generate specific immune response, therefore safety is poor；Though the latter's safety is good, transfection efficiency is low, thus in clinic It is restricted in.Some researches show that use ultrasonic microbubble as genophore, be then a kind of not only safety, transfection efficiency but also high Approach.

The outer membrane of acoustic contrast agent microvesicle is mainly door albumen or lipid, and kernel is mainly the fluorine-containing indifferent gas of low solubility Body, diameter is less than 10um, and performance is stablized, and each histoorgan of whole body can be reached by pulmonary circulation, and microvesicle is acted in incident acoustic wave The metamorphosis such as compression, expansion, broken occur for lower resonance, and generate microbeam in shattering process, go out wave and injection stream, make Adjacent cells film or a large amount of slight voids (i.e. cavitation effect) of endangium surface generation, increase cell membrane or endangium lead to Permeability, to improve gene transfection efficiency.

A kind of ultrasound microbubble mediated gene transfection auxiliary device is disclosed in the related technology, which is applied to laboratory, Carry out ultrasound microbubble mediated gene transfection experiments comprising open big bottle, the top of open big bottle be provided be arranged above and below it is more A scale slot, a pair adjust iron wire, a pair of of carrying iron wire and the culture dish being placed on carrying iron wire, and carrying iron wire is fixed on big bottle Interior, adjusting iron wire, which is located under carrying iron wire and takes, to be placed on scale slot, and the ultrasonic microbubble quantity generated is relatively fewer, causes to turn It is poor to contaminate effect.

Utility model content

The utility model is intended to solve at least some of the technical problems in related technologies.For this purpose, this reality It is to propose a kind of transfected by ultrasound mediated destruction of microbubbles device with novel purpose.

To achieve the above object, according to the transfected by ultrasound mediated destruction of microbubbles device of the utility model embodiment, comprising:

Liquid storage container, the liquid storage container is to contain acoustic contrast agent；

Kickboard, the kickboard are located in the liquid storage container and float in the acoustic contrast agent；

Ultrasonic transducer, the ultrasonic transducer are installed in the kickboard, and are hanged under the buoyancy of the kickboard It floats in the acoustic contrast agent；

Culture dish, the culture dish are suspended at the top in the liquid storage container and being located at the ultrasonic transducer.

According to transfected by ultrasound mediated destruction of microbubbles device provided by the embodiment of the utility model, ultrasonic transducer is outstanding by kickboard It floats in acoustic contrast agent, in this way, not contacting or fixing with other fixing pieces, in ultrasonication in the ultrasound in liquid storage container When contrast agent, ultrasonic energy is passed directly in acoustic contrast agent, there is no energy loss, so that acoustic contrast agent generates The amount of microvesicle is bigger, and then transfection can be improved.

In addition, can also be had according to the transfected by ultrasound mediated destruction of microbubbles device of the utility model above-described embodiment following attached The technical characteristic added:

One embodiment according to the present utility model, further includes:

Bracket, the liquid storage container are removably mounted on the bracket, and the culture dish can be upper and lower by elevating mechanism It is slidingly arranged on the bracket.

One embodiment according to the present utility model, the bracket include pedestal, the first strut, the second strut and microscope carrier, First strut and the second strut are relatively fixed on the pedestal and vertically extend, and the microscope carrier is fixedly arranged on described Between first strut and the second strut, the liquid storage container is set on the microscope carrier.

One embodiment according to the present utility model, the elevating mechanism include:

First screw rod and the second screw rod, first strut are equipped with the first sliding groove that vertically extends, and second Bar is equipped with the second sliding slot vertically extended, and first screw rod is set in the first sliding groove and can be along its own Axis pivots, and second screw rod is set in the second sliding slot and can pivot along its own axis；

First sliding block and the second sliding block, first sliding block are slideably installed in the first sliding groove and with described the One wire rod thread set is matched, second sliding block be slideably installed in the second sliding slot and with the second wire rod thread set Match；

Pallet, the two sides of the pallet are respectively provided with a linking arm, one of two described linking arms and first sliding block It is fixedly connected, another in two linking arms is fixedly connected in second sliding block；The culture dish is set to described On pallet.

One embodiment according to the present utility model, the elevating mechanism further include the first in command and second handle, described The first in command is located at the upper end of first screw rod, drives first screw rod to rotate with so that user operates, and described second Handle is located at the upper end of second screw rod, drives second screw rod to rotate with so that user operates.

One embodiment according to the present utility model, the bottom of the liquid storage container are equipped with one and are connected to ultrasonic generator Waterproof port, the ultrasonic transducer have a connecting line extended from up to down, and the free end of the connecting line has grafting Head, the connection-peg are plugged with the waterproof port.

One embodiment according to the present utility model further includes gear pump, and the entrance of the gear pump is connected to the storage Liquid container and air machinery, the outlet of the gear pump are connected to the liquid storage container, will surpass in the liquid storage container Sound contrast agent extraction after be passed through again after the uniform mixed foaming of inert gas into the liquid storage container.

One embodiment according to the present utility model, the gear pump include the pump housing, first gear, second gear and driving Axis is formed with pump chamber in the pump housing, and the first gear is meshed with the second gear and is located in the pump chamber, described The two sides of first gear and the second gear engagement place are respectively formed admission chamber and exhaust chamber；

There is the feed pathway being connected with the admission chamber and the row being connected with the exhaust chamber on the pump housing Liquid channel, the feed pathway are connected with the liquid storage container and air machinery；

The drive shaft and the first gear are socketed, to drive the first gear to rotate, so that the feed pathway The acoustic contrast agent and inert gas of inflow flow to the exhaust chamber from the admission chamber, and flow to the row from the exhaust chamber Liquid channel.

The additional aspect and advantage of the utility model will be set forth in part in the description, partially will be from following description In become obvious, or recognized by the practice of the utility model.

Detailed description of the invention

In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor Under, the structure that can also be shown according to these attached drawings obtains other attached drawings.

Fig. 1 is the structural schematic diagram of the utility model embodiment transfected by ultrasound mediated destruction of microbubbles device；

Fig. 2 is the cross-sectional view of the utility model embodiment transfected by ultrasound mediated destruction of microbubbles device；

Fig. 3 is the exploded view of the utility model embodiment transfected by ultrasound mediated destruction of microbubbles device；

Schematic diagram when Fig. 4 is the utility model embodiment transfected by ultrasound mediated destruction of microbubbles device experimental state；

Fig. 5 is the structural schematic diagram of another embodiment transfected by ultrasound mediated destruction of microbubbles device of the utility model；

Fig. 6 is the structural schematic diagram of another embodiment transfected by ultrasound mediated destruction of microbubbles device middle gear pump of the utility model.

Appended drawing reference:

Liquid storage container 10；

Waterproof port 101；

Kickboard 20；

Ultrasonic transducer 21；

Connecting line 211；

Connection-peg 212；

Culture dish 30；

Elevating mechanism 31；

First screw rod 311；

Second screw rod 312；

First sliding block 313；

Second sliding block 314；

Pallet 315；

Linking arm 3151；

The first in command 316；

Second handle 317；

Bracket 40；

Pedestal 401；

First strut 402；

Second strut 403；

Microscope carrier 404；

Gear pump 50；

The pump housing 501；

First gear 502；

Second gear 503；

Drive shaft 504；

Pump chamber P50；

Admission chamber P501；

Exhaust chamber P502；

Feed pathway H501；

Apocenosis passage H502；

Air machinery 60.

The embodiments will be further described with reference to the accompanying drawings for the realization, functional characteristics and advantage of the utility model aim.

Specific embodiment

The embodiments of the present invention are described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning Same or similar element or element with the same or similar functions are indicated to same or similar label eventually.Below by ginseng The embodiment for examining attached drawing description is exemplary, it is intended to for explaining the utility model, and should not be understood as to the utility model Limitation, based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts Every other embodiment obtained, fall within the protection scope of the utility model.

In the description of the present invention, it should be understood that term " center ", " longitudinal direction ", " transverse direction ", " length ", " width Degree ", " thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " suitable The orientation or positional relationship of the instructions such as hour hands ", " counterclockwise " " axial direction ", " circumferential direction ", " radial direction " is orientation based on the figure Or positional relationship, be merely for convenience of describing the present invention and simplifying the description, rather than the device of indication or suggestion meaning or Element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as the limit to the utility model System.

In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or Implicitly include one or more of the features.The meaning of " plurality " is two or two in the description of the present invention, More than, unless otherwise specifically defined.

In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " Gu It is fixed " etc. terms shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood as the case may be Concrete meaning in the present invention.

In the present invention unless specifically defined or limited otherwise, fisrt feature the "upper" of second feature or it "lower" may include that the first and second features directly contact, and also may include that the first and second features are not direct contacts but lead to Cross the other characterisation contact between them.Moreover, fisrt feature includes above the second feature " above ", " above " and " above " One feature is right above second feature and oblique upper, or is merely representative of first feature horizontal height higher than second feature.First is special Sign is directly below and diagonally below the second feature including fisrt feature under the second feature " below ", " below " and " below ", or only Indicate that first feature horizontal height is less than second feature.

The transfected by ultrasound mediated destruction of microbubbles device of the utility model embodiment is described in detail with reference to the accompanying drawings.

It referring to figs. 1 to 4, can according to transfected by ultrasound mediated destruction of microbubbles device provided by the embodiment of the utility model To be tested for transfected by ultrasound mediated destruction of microbubbles comprising liquid storage container 10, kickboard 20, ultrasonic transducer 21 and culture dish 30.

Specifically, liquid storage container 10 is to contain acoustic contrast agent.Kickboard 20 is located in the liquid storage container 10 and floats on In the acoustic contrast agent.Ultrasonic transducer 21 is installed in the kickboard 20, and is hanged under the buoyancy of the kickboard 20 It floats in the acoustic contrast agent.Culture dish 30 is suspended in the liquid storage container 10 and is located at the upper of the ultrasonic transducer 21 Side.

That is, kickboard 20 is located in liquid storage container 10 and the buoyancy upward by acoustic contrast agent, which can To be less than the material, such as plastics, foamed material etc. of acoustic contrast agent using density, and ultrasonic transducer 21 is mounted on kickboard 20 On, such as be set in kickboard 20, in this way, the buoyancy using kickboard 20 can make ultrasonic transducer 21 be suspended in ultrasonic contrast In agent.Culture dish 30 is suspended in liquid storage container 10, and is located at the top of ultrasonic transducer 21.

When specifically used, acoustic contrast agent is added in liquid storage container 10, and makes the liquid level of acoustic contrast agent slightly higher In or be flush to the bottom surface of culture dish 30, there is target cell in culture dish 30, in addition, being passed through inertia into liquid storage container 10 Gas, such as fluorine, nitrogen etc..When ultrasonic transducer 21 works, ultrasonic transducer 21 generates vibration, and energy is directly conducted to Acoustic contrast agent constantly squeezes acoustic contrast agent, so that acoustic contrast agent generates the variation of pressure, the variation meeting of this pressure The air pocket for generating the inert gas dissolved in is constantly disturbed, and is finally broken into microbubble and is added to acoustic contrast agent In.

According to transfected by ultrasound mediated destruction of microbubbles device provided by the embodiment of the utility model, ultrasonic transducer 21 passes through kickboard 20 are suspended in acoustic contrast agent, in this way, not contacting or fixing with other fixing pieces, in ultrasonication in liquid storage container 10 Acoustic contrast agent when, ultrasonic energy is passed directly in acoustic contrast agent, there is no energy loss, so that ultrasonic contrast The amount that agent generates microvesicle is bigger, and then transfection can be improved.

It referring to figs. 1 to 4, further include bracket 40, liquid storage container 10 can in one embodiment of the utility model Disassembly is installed on the bracket 40, and the culture dish 30 can be slided up and down to by elevating mechanism 31 is set to the bracket 40 On.

That is, culture dish 30 can be can be adjusted up and down in height by elevating mechanism 31, and then adjusts culture dish 30 and surpass Distance between sonic transducer 21, in this way, the distance between culture dish 30 and ultrasonic transducer 21 can be adjusted according to requirement of experiment, And then observation analysis transfection, meet experiment use demand.

In a specific embodiment of the utility model, bracket 40 includes pedestal 401, the first strut 402, the second strut 403 and microscope carrier 404, first strut 402 and the second strut 403 are relatively fixed on the pedestal 401 and along the vertical direction Extend, the microscope carrier 404 is fixedly arranged between first strut 402 and the second strut 403, and the liquid storage container 10 is set to institute It states on microscope carrier 404.

That is, the first strut 402 and the second strut 403 are oppositely arranged and vertically extend, microscope carrier 404 is installed Between first support 40 and second support 40, to carry liquid storage container 10, fixed in this way, being convenient to liquid storage container 10, and And be convenient to liquid storage container 10 and be in a relatively high height and position, it is convenient for experimental implementation and observation.

Referring to shown in Fig. 2 to Fig. 3, in one embodiment of the utility model, elevating mechanism 31 includes the first screw rod 311, the second screw rod 312, the first sliding block 313, the second sliding block 314 and pallet 315, wherein the first strut 402 is equipped with vertically The first sliding groove that direction extends, the second strut 403 are equipped with the second sliding slot vertically extended, first screw rod 311 It is set in the first sliding groove and can be pivoted along its own axis, second screw rod 312 is set in the second sliding slot And it can be pivoted along its own axis.

First sliding block 313 is slideably installed in the first sliding groove and matches with 311 thread bush of the first screw rod, institute The second sliding block 314 is stated slideably to be installed in the second sliding slot and match with 312 thread bush of the second screw rod.

The two sides of pallet 315 are respectively provided with a linking arm 3151, and one of two described linking arms 3151 are sliding with described first Block 313 is fixedly connected, another in two linking arms 3151 is fixedly connected in second sliding block 314；The culture Ware 30 is set on the pallet 315.

That is, the first screw rod 311 and the second screw rod 312 are pivotally arranged in first sliding groove and second sliding slot respectively Interior, the first sliding block 313 and the second sliding block 314 are equipped with perforative threaded hole, and the first sliding block 313 and the second sliding block 314 respectively can Sliding is located in first sliding groove and second sliding slot, and the threaded hole thread bush of the first sliding block 313 fits on the first screw rod 311, The threaded hole thread bush of second sliding block 314 fits on the second screw rod 312.And two linking arms 3151 of pallet 315 are separately connected On the first sliding block 313 and the second sliding block 314.

When the first screw rod 311 and the second screw rod 312 rotate, the first screw rod 311 drives the first sliding block 313 in first sliding groove It inside slides up and down, the second screw rod 312 drives the second sliding block 314 to slide up and down in second sliding slot, due to two connections of pallet 315 Arm 3151 is connected on the first sliding block 313 and the second sliding block 314, so, when on the first sliding block 313 and the second sliding block 314 When lower slider, pallet 315 can be driven to move up and down and then adjust height and position, structure is simple, and it is easy to assembly, also, energy Enough reliablely and stablely driving pallets 315 move up and down, and then reach accurately, reliable 315 height of adjusting tray.

Illustratively, first sliding groove and second sliding slot can be configured to dovetail groove, i.e. section of first sliding groove and second sliding slot Face is formed as trapezoidal, corresponding, and the first sliding block 313 and the second sliding block 314 can be configured to dovetail shaped compatible with dovetail groove, It is assembled respectively with first sliding groove and second sliding slot in this way, being convenient to the first sliding block 313 and the second sliding block 314, it is also possible to really Protect the first sliding block 313 and the second sliding block 314 slide up and down it is relatively reliable.

Advantageous, in an example of the utility model, elevating mechanism 31 further includes the first in command 316 and second handle 317, the first in command 316 is located at the upper end of first screw rod 311, drives first screw rod with so that user operates 311 rotations, the second handle 317 are located at the upper end of second screw rod 312, operate driving described second with for user Screw rod 312 rotates.

When in use, it can be rotated by holding the first in command 316 and second handle 317, and then drive the first screw rod 311 and second screw rod 312 rotate, in this way, can be convenient in experimentation, pass through the first in command 316 and second handle 317 rotate The height and position of culture dish 30 is operated and adjusts, it is more convenient using adjusting.

Referring to shown in Fig. 2 and Fig. 4, in one embodiment of the utility model, the bottom of liquid storage container 10 is equipped with one and connects It is connected to the waterproof port 101 of ultrasonic generator, the ultrasonic transducer 21 has a connecting line 211 extended from up to down, institute The free end for stating connecting line 211 has connection-peg 212, and the connection-peg 212 is plugged with the waterproof port 101.

That is, the connection-peg 212 on the connecting line 211 of ultrasonic transducer 21 can be plugged to 10 bottom of liquid storage container Waterproof port 101 connection-peg 212 on connecting line 211 is first plugged to waterproof port 101 in this way, when in use, then Acoustic contrast agent is added in liquid storage container 10, in this way, ultrasonic transducer 21 can be connected to ultrasonic generator, and it is possible to It avoids connecting ultrasonic transducer 21 in from outside lead to liquid storage container 10, and causes the shadow to 21 energy transmission of ultrasonic transducer It rings.

It referring to Figure 5, further include gear pump 50 in some embodiments of the utility model, the gear pump 50 Entrance is connected to the liquid storage container 10 and air machinery 60, and the outlet of the gear pump 50 is connected to the liquid storage container 10, To by the liquid storage container 10 acoustic contrast agent extraction after be passed through again after the uniform mixed foaming of inert gas to described In liquid storage container 10.

That is, liquid storage container 10 has liquid outlet and inlet, liquid outlet is connected with the entrance of gear pump 50, feed liquor Mouth is connected with the outlet of gear pump 50, meanwhile, the entrance of gear pump 50 is also connected with external air machinery 60, in this way, gear When 50 work of pump, the acoustic contrast agent in liquid storage container 10 is sucked, also, passes through external air machinery 60 for inert gas It being passed through to gear pump 50, inert gas and acoustic contrast agent are passed through after evenly mixing to liquid storage container 10 by gear pump 50 again, Due to the effect of gear pump 50, inert gas can uniformly be mixed into acoustic contrast agent, so that having in acoustic contrast agent There is relatively more uniform bubble, in this way, the acoustic contrast agent with uniform bubble, again under the action of ultrasound, rupture forms more Microbubble and dissolve in acoustic contrast agent so that the microbubble in acoustic contrast agent is more more evenly.

Referring to shown in Fig. 6, in one embodiment of the utility model, gear pump 50 includes the pump housing 501, first gear 502, second gear 503 and drive shaft 504, are formed with pump chamber P50 in the pump housing 501, the first gear 502 and described the Two gears 503 are meshed and are located in the pump chamber P50, and the two of 503 engagement place of the first gear 502 and the second gear Side is respectively formed admission chamber P501 and exhaust chamber P502.

On the pump housing 501 have with the admission chamber P501 feed pathway H501 being connected and with the exhaust chamber P502 The apocenosis passage H502 being connected, the feed pathway H501 are connected with the liquid storage container 10 and air machinery 60.

Drive shaft 504 and the first gear 502 are socketed, to drive the first gear 502 to rotate, so that the feed liquor The acoustic contrast agent and inert gas that channel H501 is flowed into flow to the exhaust chamber P502 from the admission chamber P501, and from described Exhaust chamber P502 flows to the apocenosis passage H502.

When specifically used, drive shaft 504 is connected to motor, is rotated by the motor driven drive shaft 504, drive shaft 504 drive first gears 502 to rotate, and first gear 502 drives second gear 503 to rotate again, in this way, first gear 502 and the Negative pressure is formed in the admission chamber P501 of two gears, 503 side, so that inert gas and acoustic contrast agent enter to the admission chamber P501, and engaged along with the rotation of first gear 502 and second gear 503, first gear 502 and second gear 503 Inter-tooth volume constantly increases diminution, so that inert gas and acoustic contrast agent are discharged to exhaust chamber P502, shape in exhaust chamber P502 At positive pressure, the apocenosis passage H502 can be finally flowed to from exhaust chamber P502, and liquid storage is back to by apocenosis passage H502 Container 10.

It should be noted that being held since the inter-tooth volume of first gear 502 and second gear 503 is minimum by the between cog Long-pending compression, so that inert gas and acoustic contrast agent be by full and uniform mixing, and then forms a large amount of bubble, in this way, can To improve the quantity and uniformity of bubble.

In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example Point is contained at least one embodiment or example of the utility model.In the present specification, to the schematic table of above-mentioned term It states and is necessarily directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be with It can be combined in any suitable manner in any one or more of the embodiments or examples.In addition, without conflicting with each other, this field Technical staff can by the feature of different embodiments or examples described in this specification and different embodiments or examples into Row combination and combination.

The above is only the preferred embodiment of the present invention, and therefore it does not limit the scope of the patent of the utility model, Under all utility models in the utility model are conceived, equivalent structure made based on the specification and figures of the utility model Transformation, or directly/be used in other related technical areas indirectly and be included in the scope of patent protection of the utility model.

Claims (8)

1. a kind of transfected by ultrasound mediated destruction of microbubbles device characterized by comprising

Liquid storage container, the liquid storage container is to contain acoustic contrast agent；

Kickboard, the kickboard are located in the liquid storage container and float in the acoustic contrast agent；

Ultrasonic transducer, the ultrasonic transducer are installed in the kickboard, and are suspended under the buoyancy of the kickboard In the acoustic contrast agent；

Culture dish, the culture dish are suspended at the top in the liquid storage container and being located at the ultrasonic transducer.

2. transfected by ultrasound mediated destruction of microbubbles device according to claim 1, which is characterized in that further include:

Bracket, the liquid storage container are removably mounted on the bracket, and the culture dish can be slided up and down by elevating mechanism Ground is arranged on the bracket.

3. transfected by ultrasound mediated destruction of microbubbles device according to claim 2, which is characterized in that the bracket include pedestal, First strut, the second strut and microscope carrier, first strut and the second strut be relatively fixed on the pedestal and vertically side To extension, the microscope carrier is fixedly arranged between first strut and the second strut, and the liquid storage container is set on the microscope carrier.

4. transfected by ultrasound mediated destruction of microbubbles device according to claim 3, which is characterized in that the elevating mechanism includes:

First screw rod and the second screw rod, first strut are equipped with the first sliding groove that vertically extends, on the second strut Equipped with the second sliding slot vertically extended, first screw rod is set in the first sliding groove and can be along its own axis It pivots, second screw rod is set in the second sliding slot and can pivot along its own axis；

First sliding block and the second sliding block, first sliding block be slideably installed in the first sliding groove and with described first Bar thread bush is matched, and second sliding block is slideably installed in the second sliding slot and matches with the second wire rod thread set；

Pallet, the two sides of the pallet are respectively provided with a linking arm, and one of two described linking arms are fixed with first sliding block It connects, another in two linking arms is fixedly connected in second sliding block；The culture dish is set to the pallet On.

5. transfected by ultrasound mediated destruction of microbubbles device according to claim 4, which is characterized in that the elevating mechanism further includes The first in command and second handle, the first in command are located at the upper end of first screw rod, operate driving institute with for user The rotation of the first screw rod is stated, the second handle is located at the upper end of second screw rod, operates driving described the with for user The rotation of two screw rods.

6. transfected by ultrasound mediated destruction of microbubbles device according to claim 1, which is characterized in that the bottom of the liquid storage container The waterproof port for being connected to ultrasonic generator equipped with one, the ultrasonic transducer have a connecting line extended from up to down, institute The free end for stating connecting line has connection-peg, and the connection-peg is plugged with the waterproof port.

7. transfected by ultrasound mediated destruction of microbubbles device according to claim 1, which is characterized in that it further include gear pump, it is described The entrance of gear pump is connected to the liquid storage container and air machinery, and the outlet of the gear pump is connected to the liquid storage container, To by the liquid storage container acoustic contrast agent extraction after be passed through again after the uniform mixed foaming of inert gas to the storage In liquid container.

8. transfected by ultrasound mediated destruction of microbubbles device according to claim 7, which is characterized in that the gear pump includes pump Body, first gear, second gear and drive shaft are formed with pump chamber, the first gear and the second gear in the pump housing Be meshed and be located in the pump chamber, the two sides of the first gear and the second gear engagement place be respectively formed admission chamber and Exhaust chamber；

Have the feed pathway being connected with the admission chamber and the drain being connected with the exhaust chamber logical on the pump housing Road, the feed pathway are connected with the liquid storage container and air machinery；

The drive shaft and the first gear are socketed, to drive the first gear to rotate, so that the feed pathway flows into Acoustic contrast agent and inert gas from the admission chamber flow to the exhaust chamber, and it is logical from the exhaust chamber to flow to the drain Road.