CN208476792U - Optical detection apparatus - Google Patents
Optical detection apparatus Download PDFInfo
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- CN208476792U CN208476792U CN201821232276.2U CN201821232276U CN208476792U CN 208476792 U CN208476792 U CN 208476792U CN 201821232276 U CN201821232276 U CN 201821232276U CN 208476792 U CN208476792 U CN 208476792U
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- reflecting mirror
- optical detection
- detection apparatus
- lens
- light beam
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Abstract
The utility model discloses a kind of optical detection apparatus, wherein, the optical detection apparatus includes mounting base, laser, the first reflecting mirror, the second reflecting mirror, condenser lens, capillary module, objective lens and third reflecting mirror have been sequentially arranged at the marginal position of mounting base, the excitation beam that laser generates is irradiated the capillary in capillary module after the first reflecting mirror, the second reflecting mirror and condenser lens, to generate fluorescence；The middle position of mounting base is provided with optical filter, sleeve lens and spectrometer, and objective lens collect the fluorescent light beam generated in capillary, and fluorescent light beam enters in spectrometer after third reflecting mirror, optical filter and sleeve lens, to be tested and analyzed.The optical detection apparatus of the utility model is reasonably laid out by the positional relationship to each component, and makes full use of the reflex of reflecting mirror to adjust optical path, has been saved installation space, has been increased the integration of device, has realized the target of device miniaturization.
Description
Technical field
The utility model relates to biochemistry detection field more particularly to a kind of optical detection apparatus.
Background technique
Capillary electrophoresis detection technology is used as main analytical technology a kind of now, efficient by its, sensitive, quick, set
The features such as standby simple, strong applicability, it is widely used in every field.It is one kind using capillary as split tunnel, with high straightening
Galvanic electricity field is the novel liquid-phase isolation technics of driving force.Capillary Electrophoresis actually includes electrophoresis, chromatography and its intersects content, it
Enable analytical chemistry from microlitre horizontally entering into a nanoliter level, and makes it possible single cell analysis or even single molecule analysis.
It is mostly at present to combine capillary electrophoresis technique with Fluorometric assay of fluorescence-labeled technology, to contained in test sample
Substance to be checked is detected.It specifically, is that fluorescent dye is marked in biomolecule, what biomolecule moved in capillary
In the process, fluorescent dye is generated fluorescence by light source activation, and detector receives generated fluorescence, and the received fluorescence of analysis institute comes
Detect the type or content of biomolecule in sample in capillary.
With the fast development of gene diagnosis, GM food detection, personalized medicine etc., present Capillary Electrophoresis inspection
Optical detection apparatus in survey technology has gradually been unable to satisfy every needs of current biotechnology, especially minimizes, collects
Cheng Hua, facilitation demand.
Utility model content
The purpose of this utility model is to provide a kind of high degree of automations, easy to operate, optical detection easy to use
Device.
To achieve the above object, the optical detection apparatus of the utility model the specific technical proposal is:
A kind of optical detection apparatus, wherein including mounting base, be sequentially arranged laser at the marginal position of mounting base
Device, the first reflecting mirror, the second reflecting mirror, condenser lens, capillary module, objective lens and third reflecting mirror, laser generate
Excitation beam the capillary in capillary module is shone after the first reflecting mirror, the second reflecting mirror and condenser lens
It penetrates, to generate fluorescence；The middle position of mounting base is provided with optical filter, sleeve lens and spectrometer, and objective lens are collected
The fluorescent light beam generated in capillary, fluorescent light beam enter in spectrometer after third reflecting mirror, optical filter and sleeve lens,
To be tested and analyzed.
Further, laser, the first reflecting mirror, the second reflecting mirror, condenser lens, capillary module, objective lens and
Three reflecting mirrors are circumferentially positioned at the outside of optical filter, sleeve lens and spectrometer.
Further, laser is extended along the first long side of mounting base, close to the hair of laser on mounting base
It penetrates at the position at end and is provided with the first reflecting mirror, formed between the excitation beam that the reflecting surface and laser of the first reflecting mirror issue
There is the first reflection angle, the first reflection angle can be such that excitation beam is reflected on the second reflecting mirror by the first reflecting mirror.
Further, the first reflecting mirror is arranged in the first long side of mounting base and the intersection of the first short side, and second
The first short side of mounting base and the intersection of the second long side is arranged in reflecting mirror, and the first reflecting mirror reflects primary anti-
The second reflection angle is formed between irradiating light beam and the reflecting surface of the second reflecting mirror, the second reflection angle can make primary event light beam
It is reflected on condenser lens by the second reflecting mirror.
Further, the first reflection angle and the second reflection angle are 45 °.
Further, condenser lens and capillary module are arranged in the second long side of mounting base, and condenser lens is located at the
Between two-mirror and capillary module.
Further, the optical detection window face in objective lens and capillary is arranged, to collect at optical detection window
The fluorescent light beam of sending, third reflecting mirror are arranged at the light outlet of objective lens, the reflecting surface of third reflecting mirror and detection
Third reflection angle is formed between the fluorescent light beam that object lens issue, third reflection angle can be such that fluorescent light beam reflects by third
Mirror is reflected on optical filter and sleeve lens.
Further, third reflection angle is 45 °.
Further, it is provided with optical filter at the light entrance of sleeve lens, light outlet is connected with spectrometer.
Further, at least one of the first reflecting mirror and third reflecting mirror are arranged on adjustable eyeglass platform.
The optical detection apparatus of the utility model has the advantage that be carried out reasonably by the positional relationship to each component
Layout, and the reflex of reflecting mirror is made full use of to adjust optical path, installation space has been saved, the integration of device is increased,
Realize the target of device miniaturization.
Detailed description of the invention
Fig. 1 is the perspective view one of the optical detection apparatus of the utility model；
Fig. 2 is the perspective view two of the optical detection apparatus of the utility model；
Fig. 3 is the index path of the optical detection apparatus of the utility model.
Specific embodiment
In order to be better understood by the purpose of this utility model, structure and function, with reference to the accompanying drawing, to the utility model
A kind of optical detection apparatus does further detailed description.
As depicted in figs. 1 and 2, the optical detection apparatus of the utility model includes mounting base 1, and mounting base 1 is rectangle
Structure, laser 2, the first reflecting mirror 3, the second reflecting mirror 4, condenser lens 5, capillary module 6, objective lens 7 and third are anti-
It penetrates mirror 8 to be arranged at the marginal position of mounting base 1, optical filter 9, sleeve lens 10 and spectrometer 11 are located at mounting base 1
Middle position.The excitation beam in the optical detection apparatus of the utility model is in the marginal position for being located at mounting base as a result,
It is propagated between each component at place, and the capillary in capillary module is irradiated, to generate fluorescence, and the fluorescence generated is then
It can be propagated between each component of middle position for being located at mounting base, enter eventually into and complete to test and analyze in spectrometer.
Further, as shown in figures 1 and 3, laser 2 is arranged at the one side edge of mounting base 1, it is preferred that swashs
Light device 2 is extended along the first long side 12 of mounting base 1, wherein laser 2 is used to provide excitation beam 16, exciting light
Beam 16 is propagated along the first long side 12 of mounting base 1, and exciting light can have an effect with the fluorescent reagent in capillary, to generate
Fluorescence realizes the optical detection of Capillary Electrophoresis.
Further, the first reflecting mirror 3 is provided at the position of the transmitting terminal of laser 2 on mounting base 1, wherein
The first reflection angle, the first reflection folder are formed between the excitation beam 16 that the reflecting surface and laser 2 of first reflecting mirror 3 issue
Angle can be such that excitation beam 16 is reflected on subsequent second reflecting mirror 4 by the first reflecting mirror 3, it is preferred that the first reflection folder
Angle is 45 °, so that excitation beam 16 at right angles reflects at the first reflecting mirror 3.Specifically, as shown in figures 1 and 3, first
The first corner of mounting base 1 is arranged in reflecting mirror 3, that is, the intersection of the first long side 12 and the first short side 13, by
This, laser 2 along mounting base 1 the first long side 12 issue excitation beam 16 can by the first reflecting mirror 3 reflection and
Become the primary event light beam 17 propagated along the first short side 13 of mounting base 1.
Further, as shown in Figures 2 and 3, the second corner of mounting base 1 is arranged in the second reflecting mirror 4, that is, the
The intersection of one short side 13 and the second long side 14, the primary event light beam 17 and the second reflection that the first reflecting mirror 3 reflects
The second reflection angle is formed between the reflecting surface of mirror 4, the second reflection angle can make primary event light beam 17 pass through the second reflection
Mirror 4 is reflected on subsequent condenser lens 5, it is preferred that the second reflection angle is also 45 °, so that primary event light beam 17 exists
It is equally at right angles reflected at second reflecting mirror 4.
Further, as shown in Figures 2 and 3, the second long side of mounting base 1 is arranged in condenser lens 5 and capillary module 6
Side 14, wherein condenser lens 5 can receive the second reflecting mirror 4 and reflect back between the second reflecting mirror 4 and capillary module 6
Secondary reflection light beam 18, and be irradiated on the capillary in capillary module 6 after forming focus on light beam 19, specially capillary
On optical detection window at, to generate fluorescence.Specifically, capillary module 6 can be located at the third turning of mounting base 1
Place, that is, the intersection of the second long side 14 and the second short side 15.
Further, objective lens 7 are set together with capillary module 6, specifically, in objective lens 7 and capillary
Optical detection window face setting, with collect at optical detection window issue fluorescent light beam 20.In addition, third reflecting mirror 8
It is arranged at the light outlet of objective lens 7, wherein the fluorescent light beam that the reflecting surface and objective lens 7 of third reflecting mirror 8 issue
Third reflection angle is formed between 20, it is subsequent that third reflection angle can be such that fluorescent light beam 20 is reflected by third reflecting mirror 8
Optical filter 9 and sleeve lens 10 on, it is preferred that third reflect angle be 45 ° so that fluorescent light beam 20 is in third reflecting mirror
It is at right angles reflected at 8.
Further, optical filter 9 is provided at the light entrance of sleeve lens 10, light outlet is connected with spectrometer 11,
In, optical filter 9 is used to will go into impurity light beam (such as excitation beam) mistake in the triple reflection light beam 21 of sleeve lens 10
It filters, to form detection light beam, guarantees the accuracy tested and analyzed；Sleeve lens 10 are used to will test after light beam focuses again to pass
It is defeated into spectrometer 11；Spectrometer 11 is used to carry out spectrum analysis to the detection light beam after focusing.
As a result, as shown in figure 3, the course of work of the optical detection apparatus of the utility model are as follows: 1) laser 2 issues excitation
Light beam 16；2) excitation beam 16 forms primary event light beam 17 by the reflection of the first reflecting mirror 3；3) primary event light beam 17
It is irradiated on the second reflecting mirror 4, forms secondary reflection light beam 18；4) secondary reflection light beam 18 is irradiated on condenser lens 5, is formed
Focus on light beam 19；5) focus on light beam 19 is irradiated on the capillary in capillary module 6, generates fluorescence；6) objective lens 7 are collected
The fluorescent light beam 20 generated in capillary；7) fluorescent light beam 20 is irradiated on third reflecting mirror 8, forms triple reflection light beam 21；
8) triple reflection light beam 21 is irradiated on optical filter 9, forms detection light beam；9) detection light beam passes after the focusing of sleeve lens 10
It is defeated to be tested and analyzed into spectrometer 11.
It is further preferred that at least one of the first reflecting mirror 3 and third reflecting mirror 8 are arranged in adjustable eyeglass
On platform, also the angle of the first reflecting mirror 3 and third reflecting mirror 8 can be adjusted by the way that eyeglass platform is adjusted, facilitate debugging, reduce
Assembly precision requirement, for example, dressed up 45.5 degree when the angle of the second reflecting mirror 4 is due to rigging error, then it can be by adjusting
First 0.5 degree of reflecting mirror, makes its optical path enter lens.Wherein, eyeglass platform is adjusted can be using angle common in this field
Fine tuning structure is specifically limited in the utility model not to this.
The optical detection apparatus of the utility model is reasonably laid out by the positional relationship to each component, and sufficiently benefit
Optical path is adjusted with the reflex of reflecting mirror, has saved installation space, increases the integration of device, realizes device miniaturization
Target.
The utility model is further described by specific embodiment above, it should be understood that, have here
The description of body, should not be construed as the restriction to the spirit and scope of the utility model, and one of ordinary skilled in the art is readding
The various modifications made after reader specification to above-described embodiment belong to the range that the utility model is protected.
Claims (10)
1. a kind of optical detection apparatus, which is characterized in that including mounting base, be sequentially arranged at the marginal position of mounting base
Laser, the first reflecting mirror, the second reflecting mirror, condenser lens, capillary module, objective lens and third reflecting mirror, laser
The excitation beam of generation carries out the capillary in capillary module after the first reflecting mirror, the second reflecting mirror and condenser lens
Irradiation, to generate fluorescence；The middle position of mounting base is provided with optical filter, sleeve lens and spectrometer, and objective lens are received
The fluorescent light beam generated in collection capillary, fluorescent light beam enter spectrometer after third reflecting mirror, optical filter and sleeve lens
In, to be tested and analyzed.
2. optical detection apparatus according to claim 1, which is characterized in that laser, the first reflecting mirror, the second reflection
Mirror, condenser lens, capillary module, objective lens and third reflecting mirror are circumferentially positioned at optical filter, sleeve lens and spectrometer
Outside.
3. optical detection apparatus according to claim 1 or 2, which is characterized in that first length of the laser along mounting base
Avris is extended, and is provided with the first reflecting mirror, the first reflecting mirror at the position of the transmitting terminal of laser on mounting base
Reflecting surface and the excitation beam that issues of laser between be formed with the first reflection angle, the first reflection angle can make excitation beam
It is reflected on the second reflecting mirror by the first reflecting mirror.
4. optical detection apparatus according to claim 3, which is characterized in that the of mounting base is arranged in the first reflecting mirror
The first short side and the second long side of mounting base is arranged in the intersection of one long side and the first short side, the second reflecting mirror
Intersection, be formed with the second reflection between the reflecting surface of primary event light beam and the second reflecting mirror that the first reflecting mirror reflects
Angle, the second reflection angle can be such that primary event light beam is reflected on condenser lens by the second reflecting mirror.
5. optical detection apparatus according to claim 4, which is characterized in that the first reflection angle and the second reflection angle are
45°。
6. optical detection apparatus according to claim 4, which is characterized in that condenser lens and capillary module setting are being pacified
The second long side of bottom plate is filled, condenser lens is between the second reflecting mirror and capillary module.
7. optical detection apparatus according to claim 6, which is characterized in that the optical detection in objective lens and capillary
The setting of window face, to collect the fluorescent light beam issued at optical detection window, the light of objective lens is arranged in third reflecting mirror
Line exit is formed with third reflection angle between the fluorescent light beam that the reflecting surface and objective lens of third reflecting mirror issue, the
Three reflection angles can be such that fluorescent light beam is reflected on optical filter and sleeve lens by third reflecting mirror.
8. optical detection apparatus according to claim 7, which is characterized in that it is 45 ° that third, which reflects angle,.
9. optical detection apparatus according to claim 7, which is characterized in that be provided with filter at the light entrance of sleeve lens
Mating plate, light outlet are connected with spectrometer.
10. optical detection apparatus according to claim 7, which is characterized in that in the first reflecting mirror and third reflecting mirror
At least one is arranged on adjustable eyeglass platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821232276.2U CN208476792U (en) | 2018-08-01 | 2018-08-01 | Optical detection apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821232276.2U CN208476792U (en) | 2018-08-01 | 2018-08-01 | Optical detection apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208476792U true CN208476792U (en) | 2019-02-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821232276.2U Active CN208476792U (en) | 2018-08-01 | 2018-08-01 | Optical detection apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115524345A (en) * | 2022-11-25 | 2022-12-27 | 深圳市壹倍科技有限公司 | Defect detection optical system for semiconductor |
-
2018
- 2018-08-01 CN CN201821232276.2U patent/CN208476792U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115524345A (en) * | 2022-11-25 | 2022-12-27 | 深圳市壹倍科技有限公司 | Defect detection optical system for semiconductor |
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