CN208459405U - Fiber imunosensor and the detection device detected for immunity disease - Google Patents

Abstract

The utility model provides Fiber imunosensor, including optical fiber, excitation metal film, molecular Iy self-assembled layer and monoclonal antibody layer；Excite metal film deposition on the bottom wall of the groove of optical fiber side；Molecular Iy self-assembled layer is self-assembled on excitation metal film；Monoclonal antibody layer is applied on molecular Iy self-assembled layer.Additionally provide the detection device for immunity disease detection, including sequentially connected wideband light source, Fiber imunosensor, fiber spectrometer and terminal.By fiber optic applications to surface plasmon resonance biosensor, quantitative detection can be carried out to immunological diseases marker-antigen by specific immune response, it is integrated with the advantage without label and high sensitivity of the real-time, accurate, light and handy of optical fiber sensing technology, strong antijamming capability and the advantage that can be networked and SPR sensorgram technology, energy is highly sensitive, carries out immunological diseases marker detection in real time and easily；Molecular Iy self-assembled layer can either protect excitation metal film, and can improve the packaging efficiency of monoclonal antibody.

Description

Fiber imunosensor and the detection device detected for immunity disease

Technical field

The utility model belongs to immunological diseases detection technique field, more specifically, be related to Fiber imunosensor and Detection device for immunity disease detection.

Background technique

The early diagnosis of tumour is the key that save patient vitals.

In existing diagnosing tumor technology, in addition to clinical sign and iconography means, tumor markers detection method increasingly by People pay attention to.Tumor markers are the chemical substances by tumor microenvironment stroma cell and tumor cell secretion, be present in cell, In tissue, body fluid, quantitative detection can be carried out by chemistry or the method for immune response.In general, tumour growth is more vigorous, tumour mark Will object concentration level is higher；Conversely, concentration expression is reduced when tumour growth is pressed.Detect tumor markers can than CT, The iconographies means such as MRI find tumor focus earlier, are the indispensable methods of early diagnosis.

However, carrying out infantile tumour diagnosis by detection individual event marker, there is also following weakness: first, certain tumour blood Clear concentration of the marker in early stage has no big variation, is just increased significantly to middle and advanced stage；Second, marker concentration increases and tumour Inevitable correlation there is limitation, the generation or recurrence of cancer might not be represented by increasing；Third, tumor markers Raising can occur in kinds of tumors disease, it is also necessary to which further progress targetedly checks.Early diagnosis of tumor is marker Detection is put forward new requirements: first, selection sensitivity, the higher tumor markers of specificity, the i.e. marker are swollen at certain Just there is apparent up-regulated expression in tumor early stage；Second, constantly improve detection means, transducer sensitivity, accuracy, detection pole are improved Limit, and neatly blood serum designated object can in real time, dynamically monitor.

Mesothelin (mesothelin) is the sugared egg being present on peritonaeum, pleura and pericardium body cavity mesothelium stave cell surface It is white, it is a kind of Biological indicators often having in Early pancreatic carcinoma blood samples of patients and urine.In addition, mesothelin has in several tumour Expression, for example, mesothelin be expressed in all oophoromas account for about 70%, papillary serous adenocarcinoma account for about 82%, all pancreases Gland cancer account for about 83% and all ductal adenocarcinoma of pancreas account for about 86%.Mesothelin becomes the height of the malignant tumours such as cancer of pancreas detection Specific Novel marker.

It is non-hazardous, be the new detection technique having gradually developed in the world, table without the immune biosensor technique of label Surface plasma resonance (SPR:Surface Plasmon Resonance) biosensor is exactly one of them.Without label Spr sensor be advantageous in that: it is time saving and energy saving it is not necessary that determinand is marked first, detection method is simple, can nothing Damage ground carries out real-time, dynamic monitoring to immune response；Second, detection signal is directly from immune response, in real time record reaction Combination and resolving, help to study the intermolecular interaction of antibody-antigene, mention for dynamic studies complex reaction meachanism For new possibility.But existing surface plasma body resonant vibration (SPR) detection device, such as General Electric Co. Limited BIAcore series SPR Detection device, bulky, involve great expense with optical prism (rather than optical fiber) for optical coupling medium, popularization and application are limited System.

Optical fiber sensing technology is miniaturization, highly sensitive, quickly detection application provides a kind of effective way in real time.Light Fibre sensing is detection signal with light, using optical fiber as transmission medium, using parameters such as amplitude, wavelength, the polarization states of transmission light by outer The real-time detection to extraneous factor is realized in the influence of bound variable, with highly sensitive, quick, anti-electromagnetic interference force is strong, can carry out The remarkable advantages such as long-range monitoring.In conjunction with the advantage of optical fiber sensing technology and surface plasma body resonant vibration detection technique, develop light Ingeniously, flexible, highly sensitive, the rapid optical fiber SPR sensor of response, is that realization is unmarked, harmless, Real-time and Dynamic Detection excellent Scheme is selected, and can be further applicable to remotely monitor.

2014, American Jack Andraka invented a kind of " cancer test paper " for detecting mesothelin (WO2013172866A2) it is used for the early screening of cancer of pancreas, sensitivity, response speed to be examined relative to existing conventional tumour Survey technology significantly improves.It is somebody's turn to do " cancer test paper " to be fixed on mesothelin antibody on filter paper by carbon nanotube, when mesothelin antigen Specific immune response occurs in conjunction with its antibody, the conductivity of carbon nanotube will change, by detecting electric signal The variation of indirect reaction mesothelin concentration；But the electric conductivity of the survey cancer test paper may be influenced by the factor of other non-detection objects, To influence the accuracy of true mesothelin test.

Application No. is the applications for a patent for invention of CN201610309318, and CA19-9 antibody and mesothelin antibody are passed through colloid The particles such as gold carry out revealing label and are fixed on test paper, produce the duplex detection kit of detection CA19-9 and mesothelin, Rapid screening for cancer of pancreas.This method is easy to operate, susceptibility is high, high specificity, can provide quick qualitative detection, but It can not accurate quantitative analysis measurement marker concentration.

However, using optical fiber sensing technology in conjunction with surface plasma resonance sensing technology, for realizing mesothelin etc. There is not been reported for the immunoassay technology of tumor markers.The immune biosensor technique of existing detection tumor markers can not be real When detect, can not network, can not remotely be monitored, can not internet of things oriented application in all things on earth interconnection, real-time monitoring demand.

Utility model content

The purpose of this utility model is to provide a kind of Fiber imunosensor, with solve it is existing in the prior art cannot In real time, the technical issues of accurately detecting tumor markers.

In order to achieve the above purposes, the technical solution adopted by the utility model is: Fiber imunosensor, including optical fiber, swash Send out metal film, monoclonal antibody layer and for the monoclonal antibody layer to be assembled in the molecule excited on metal film from group Fill layer；

The side of the optical fiber is equipped with groove, the fibre core of the bottom wall of the groove adjacent to the optical fiber；

The excitation metal film deposition is on the bottom wall of the groove；

The molecular Iy self-assembled layer is self-assembled on the excitation metal film；

The monoclonal antibody layer is applied on activated treated the molecular Iy self-assembled layer.

Further, the fibre core distance of the bottom wall of the groove to the optical fiber is 1~2 μm.

Further, the excitation metal film be golden film or silverskin, it is described excitation metal film with a thickness of 40~50 μm.

Further, the molecular Iy self-assembled layer is graphene nano film layer or stannic oxide/graphene nano film layer.

It further, further include the first articulamentum, first articulamentum is deposited on the bottom wall of the groove, described to swash Metal film deposition is sent out on first articulamentum.

Further, first articulamentum is chromium film, with a thickness of 2-3nm.

It further, further include the second articulamentum, second articulamentum is self-assembled on the excitation metal film, described Molecular Iy self-assembled layer is self-assembled on second articulamentum.

Further, the monoclonal antibody layer is made of the monoclonal antibody of tumor markers.

The beneficial effect of Fiber imunosensor provided by the utility model is: compared with prior art, on optical fiber Deposition excitation metal film, forms optical fiber SPR sensor, and the monoclonal being assembled on excitation metal film by molecular Iy self-assembled layer is anti- Body layer realizes the antibody modification of optical fiber SPR sensor；By fiber optic applications to surface plasmon resonance biosensor, can be exempted from by specificity Epidemic disease reaction carries out quantitative detection to immunological diseases marker-antigen, be integrated with optical fiber sensing technology it is real-time, accurate, light and handy, Strong antijamming capability and advantages and the SPR sensorgram technology such as can network without advantages such as label and sensitivity height, can it is highly sensitive, Immunological diseases marker detection is carried out in real time and easily；The bottom wall of groove help to obtain light adjacent to the fibre core of the optical fiber Strong evanscent field on fibre.

The two of the purpose of this utility model are to provide a kind of detection device for immunity disease detection, existing to solve There is the technical issues of tumor markers cannot in real time, be accurately detected present in technology.

The detection dress that in order to achieve the above purposes, the technical solution adopted by the utility model is: for immunity disease detection It sets, including wideband light source, fiber spectrometer, terminal and Fiber imunosensor, the both ends difference of the Fiber imunosensor The wideband light source and the fiber spectrometer, the terminal and the fiber spectrometer communication link are connected by connection optical fiber It connects.

The beneficial effect of detection device provided by the utility model for immunity disease detection is: with the prior art It compares, wideband light source provides the input light of covering for Fiber imunosensor, and optical signal enters Fiber imunosensor and passing Light-material effect occurs for sensillary area domain and testing liquid sample, and output optical signal is acquired by fiber spectrometer, at terminal installation data Software is managed, the resonance peak that output light is calculated is carried out to the data of fiber spectrometer acquisition, the wavelength of resonance peak can be quantitatively anti- Reflect the concentration value of immunological diseases marker；Without label, it is lossless, real-time, accurately to the tumor markers in liquid sample into Row quickly detection, and in the Internet of Things that can be further applied and need to network, remotely detect.

Detailed description of the invention

It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Attached drawing needed in description is briefly described, it should be apparent that, the accompanying drawings in the following description is only that this is practical new Some embodiments of type for those of ordinary skill in the art without any creative labor, can be with It obtains other drawings based on these drawings.

Fig. 1 is a kind of detecting state schematic diagram of Fiber imunosensor provided by the embodiment of the utility model；

Fig. 2 is the structural schematic diagram of the detection device provided by the embodiment of the utility model for immunity disease detection；

Fig. 3 is the production of Fiber imunosensor provided by the embodiment of the utility model and the schematic diagram of testing process.

Wherein, each appended drawing reference in figure:

1, Fiber imunosensor；

11, optical fiber；111, groove；12, metal film is excited；13, monoclonal antibody layer；131, antibody；14, molecular self-assembling Layer；16, the second articulamentum；17, antigen；

2, wideband light source；3, fiber spectrometer；4, terminal；5, optical fiber is connected.

Specific embodiment

In order to which technical problem to be solved in the utility model, technical solution and beneficial effect is more clearly understood, with Lower combination accompanying drawings and embodiments, the present invention will be further described in detail.It should be appreciated that specific reality described herein It applies example to be only used to explain the utility model, is not used to limit the utility model.

It should be noted that it can when element is referred to as " being fixed on " or " being set to " another element and similar term With directly on the other element or indirectly on another element.When an element be known as " being connected to " another Element, it can be directly to another element or be indirectly connected on another element.

In addition, term " first ", " second " and similar term are used for description purposes only, it is not understood to indicate or imply Relative importance or the quantity for implicitly indicating indicated technical characteristic.

Referring to Fig. 1, existing be illustrated Fiber imunosensor provided by the utility model.Fiber imunosensor 1, Including optical fiber 11, excitation metal film 12, monoclonal antibody layer 13 and for monoclonal antibody layer 13 to be assembled in excitation metal film Molecular Iy self-assembled layer 14 on 12；

The side of optical fiber 11 is equipped with groove 111, the fibre core 113 of the bottom wall adjacent fiber 11 of groove 111；

Excitation metal film 12 is deposited on the bottom wall of groove 111；

Molecular Iy self-assembled layer 14 is self-assembled on excitation metal film 12；

Monoclonal antibody layer 13 is applied on activated treated molecular Iy self-assembled layer 14.

The testing principle of the utility model Fiber imunosensor are as follows: the antibody 131 and test specimens of monoclonal antibody layer 13 Antigen 17 in this is specifically bound, and the surface refractive index at the monoclonal antibody layer 13 of Fiber imunosensor 1 is immediately Change, the surface plasma body resonant vibration resonance peak wavelength for causing 1 tag end test of Fiber imunosensor to detect occurs obvious Therefore drift can be changed by the concentration of antigen in the wavelength shift quantitative reaction test sample of resonance peak.

Fiber imunosensor provided by the utility model, compared with prior art, the deposition excitation metal on optical fiber 11 Film 12 forms optical fiber SPR sensor, is assembled in the monoclonal antibody layer 13 on excitation metal film 12 by molecular Iy self-assembled layer 14 Realize the antibody modification of optical fiber SPR sensor；Optical fiber 11 is applied to surface plasmon resonance biosensor, specific immunity can be passed through Reaction carries out quantitative detection to immunological diseases marker-antigen, is integrated with the real-time, accurate, light and handy, anti-of optical fiber sensing technology Interference performance is strong and advantages and the SPR sensorgram technology such as can network without advantages such as label and sensitivity height, can be highly sensitive, real When and easily carry out immunological diseases marker detection；The fibre core 113 of the bottom wall adjacent fiber 11 of groove 111, help to obtain Strong evanscent field on optical fiber 11.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, groove 111 The distance of fibre core 113 of bottom wall to optical fiber 11 be 1~2 μm, the strong evanscent field helping to obtain on optical fiber 11 improves immune disease The sensitivity of sick marker detection.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, excitation gold Belonging to film 12 is golden film or silverskin.

Golden film or silverskin can excite SPR resonance peak；Golden film stable chemical performance and it can directly carry out biology and repair Decorations, the disadvantage is that golden price, the SPR resonance peak of excitation and the high resolution for being not so good as silverskin；Using silverskin, more high-resolution can be obtained The SPR resonance peak of rate, and price many more cheap than golden film；

Golden film direct-assembling biomolecule it is inefficient, be arranged molecular Iy self-assembled layer 14, can be improved the assembling of antibody Efficiency；

Silverskin is easy to happen the chemical reaction such as oxidation, vulcanization in air, to influence the stabilization of Fiber imunosensor 1 Property, molecular Iy self-assembled layer 14 is set, on the one hand can protect silverskin, on the other hand can guarantee the packaging efficiency of antibody, thus Obtain the Fiber imunosensor 1 of performance height and advantage of lower cost.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, excitation gold Belong to film 12 with a thickness of 40~50 μm, obtain the SPR resonance peak of higher resolution, especially silverskin with a thickness of 40~50 μm When, the resolution ratio of SPR resonance peak significantly improves.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, molecule is certainly Assembled layers 14 are graphene nano film layer or stannic oxide/graphene nano film layer, and stable chemical performance, specific surface area are huge, to biology The adsorption capacity of molecule is stronger, and six-membered carbon ring structure is easier to form the accumulation of big pi bond in conjunction with organic molecule, relative to routine Biomolecule is assembled using golden film, graphene nano film layer or stannic oxide/graphene nano film layer can obtain higher set of biomolecules Efficiency is filled, to improve the detection sensitivity and detectable limit of Fiber imunosensor 1.

Preferably, molecular Iy self-assembled layer 14 is the graphene oxide film layer of single layer or few layer, with a thickness of 1nm~3nm, optical fiber 1 detection sensitivity of immunosensor is higher.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, further include First articulamentum, the first articulamentum are deposited on the bottom wall of groove 111, and excitation metal film 12 is deposited on the first articulamentum.The One articulamentum mainly can increase the adhesion strength of excitation metal film 12 and optical fiber 11, avoid excitation 12 peeling of metal film, demoulding.

When excitation metal film 12 is golden film or silverskin, preferably the first articulamentum is chromium film, with a thickness of 2-3nm.Golden film (or silver Film) when, by chromium transition, the adhesion strength of golden film (or silverskin) and optical fiber 11 can be significantly increased.

Further, referring to Fig. 1, a kind of specific embodiment party as Fiber imunosensor provided by the utility model Formula further includes the second articulamentum 16, and the second articulamentum 16 is self-assembled on excitation metal film 12,14 self assembly of molecular Iy self-assembled layer In on the second articulamentum 16.On the one hand the second articulamentum 16 is excitation (the especially poor chemical stability in air of metal film 12 Silverskin) provide protection, on the other hand the second articulamentum 16 is used as transition zone, connects the molecular Iy self-assembled layer of next layer of setting 14, improve the adhesion strength of excitation metal film 12 and molecular Iy self-assembled layer 14.

When exciting metal film 12 is golden film or silverskin, and when molecular Iy self-assembled layer 14 is graphene oxide layer, preferably the Two articulamentums 16 are using the positive stearylmercaptan (ODT) with a thickness of nanometer scale；

Using the ODT for containing sulfydryl as the articulamentum between golden film and graphene oxide film layer, the alkyl of golden film is realized Change, certain golden film can also be alkylated otherwise；Use the ODT that contains sulfydryl as golden film and graphene oxide film layer Between articulamentum, on silverskin, will be will form on silverskin with a thickness of the ODT self assembly of nanometer scale by Ag-S covalent bond Fine and close protective layer, can effectively overcome includes that the Fiber imunosensor 1 of silverskin is easy the shortcomings that degenerating, while also improving sharp Send out the adhesion strength of metal film 12 and molecular Iy self-assembled layer 14.

Further, as a kind of specific embodiment of Fiber imunosensor provided by the utility model, monoclonal Antibody layer 13 is made of the monoclonal antibody of tumor markers, such as mesothelin.It can be realized diagnosing tumor.

Also referring to Fig. 1 and Fig. 2, now to it is provided by the utility model for immunity disease detection detection device into Row explanation.For the detection device of immunity disease detection, including wideband light source 2, fiber spectrometer, terminal 4 and aforementioned any The Fiber imunosensor 1 that embodiment is addressed, the both ends of Fiber imunosensor 1 pass through connection optical fiber 5 respectively and connect broadband light Source 2 and fiber spectrometer, terminal 4 and fiber spectrometer communicate to connect.

Wideband light source 2 be Fiber imunosensor 1 provide covering input light (such as wave-length coverage be 400nm~ 1100nm), optical signal enters Fiber imunosensor 1 and light-material effect occurs in sensitive zones and testing liquid sample, defeated Optical signals are acquired by fiber spectrometer, terminal 4 (such as computer) installation data processing software, to the data of fiber spectrometer acquisition The resonance peak that output light is calculated is carried out, the wavelength of resonance peak can quantitative response immunological diseases marker (such as tumor-marker Object-mesothelin) concentration value；Without label, it is lossless, real-time, accurately to the tumour in liquid sample (such as blood or urine) Marker is used for quickly detecting, and in the Internet of Things that can be further applied and need to network, remotely detect.

The production method of Fiber imunosensor provided by the utility model is illustrated below.Optical fiber is immune to be passed The production method of sensor, includes the following steps:

S1, groove 111, and the fibre core 113 of the bottom wall adjacent fiber 11 of groove 111 are processed in the side of optical fiber 11；

S2, one layer of excitation metal film 12 is deposited or sputtered on the bottom wall of groove 111；

S3,12 upper surface self assembly of metal film, one layer of molecular Iy self-assembled layer 14 is being excited；

S4, anakmetomeres Iy self-assembled layer 14 make the surface of molecular Iy self-assembled layer 14 form activated carboxyl；(step S4 does not scheme Show)

S5, the amino in molecular Iy self-assembled layer 14, monoclonal antibody molecule is added dropwise in monoclonal antibody and states molecule certainly Amide reaction occurs for the activated carboxyl on the surface of assembled layers 14, so that monoclonal antibody is assembled to be fixed on molecular Iy self-assembled layer 14 Above form monoclonal antibody layer 13.

The production method of the Fiber imunosensor 1 can be used in making the immune biography of optical fiber that aforementioned any embodiment is addressed Sensor 1.This method is integrated with excitation metal film 12 and monoclonal antibody layer 13 by simple process on optical fiber 11, and selection is made Property high, sensitive Fiber imunosensor 1, packaging efficiency is high, molecular Iy self-assembled layer 14 can to excitation metal film 12 into Row protection, and metal film 12 and monoclonal antibody layer 13 is excited firmly to be attached on optical fiber 11, avoid performance from degenerating.

Further, step S1 includes: by the processing method of side-polished fiber 11, by the part of clad of optical fiber 11 112 removals are until rubbing down face removes close to 11 fibre core 113 of optical fiber and forms groove 111 at part of clad.Certain groove 111 can also It processes otherwise.

Preferably, 3000 mesh, 7000 mesh, 12000 mesh sand paper are successively used, single mode optical fiber are roughly ground, fine grinding, essence throwing Light processing and fabricating side-polished fiber.By optical power loss during monitoring 11 rubbing down of optical fiber, 11 rubbing down identity distance of optical fiber is found 1~2 μm from core region (i.e. with a distance from the fibre core 113 of the bottom wall of groove 111 to optical fiber 11 be 1~2 μm) be it is best, be conducive to obtain Obtain the strong evanscent field on optical fiber 11.

Further, step S2 includes: on side-polished fiber 11, using the side of vacuum thermal evaporation or magnetron sputtering Optical fiber SPR sensor is made in one layer of excitation metal film 12 of uniform deposition on 11 rubbing down face of optical fiber in method.

It specifically, is the adhesion strength of enhancing excitation metal film 12 and optical fiber 11, preferably first deposition first connects on optical fiber 11 Connect layer, redeposition excitation metal film 12.When exciting metal film 12 is golden film or silverskin, chromium film first is deposited on 11 surface of optical fiber, Redeposited golden film or silverskin, the thickness of chromium film concretely 2~3nm.

Further, step S3 includes:

S31, excitation metal film 12 the second articulamentum of surface self-organization 16；

S32, in the surface self-organization molecular Iy self-assembled layer 14 of the second articulamentum 16, such as graphene oxide film layer.

Further, in step S31: when exciting metal film 12 using silverskin, using positive 18 sulphur containing sulfydryl Alcohol (ODT), will be with a thickness of nanometer by Ag-S covalent bond as the second articulamentum 16 between silverskin and graphene oxide film layer The ODT self assembly of magnitude is on silverskin.

Preferably, using concentration is the ODT solution of 10mM in room temperature enclosed environment lower surface self assembly 36 hours.

Preferably, it will form on silverskin using the ODT containing sulfydryl as the second articulamentum 16 for playing middle transition Fine and close protective layer can effectively avoid the optical fiber SPR sensor based on silver from being easy the shortcomings that degenerating.

Further, in step s 32: the step S31 optical fiber SPR sensor for completing ODT self assembly is put into oxidation stone It in black alkene colloidal sol, is placed in the room temperature environment of sealing and carries out surface self-organization, ultrapure water cleaning is taken out after being completed, at room temperature Dry obtained sensitizing type optical fiber SPR sensor.

Specifically, the graphene oxide film layer self assembly time is to be advisable for 40 minutes or more, can be on the second articulamentum 16 Assemble few layer stannic oxide/graphene nano film layer.Biomolecule is assembled by few layer graphene oxide on silverskin --- Dan Ke Grand antibody, on the one hand graphene oxide film layer provides further protection for silverskin, on the other hand due to graphene oxide Stable chemical performance, specific surface area are huge, stronger to the adsorption capacity of biomolecule, and six-membered carbon ring structure is easier to and organic molecule In conjunction with the accumulation for forming big pi bond.Biomolecule is assembled relative to conventional use golden film, graphene oxide film layer can obtain higher Biomolecule assemble efficiency, to improve the detection sensitivity and detectable limit of sensor.

Specifically, graphene oxide colloidal sol can be made by following methods:

It uses water or n,N-Dimethylformamide (DMF) for solvent, the commercial graphene oxide powder of purchase is dissolved in it In stir evenly, be placed in five faces vibration ultrasonic drilling machine in, to graphene oxide solution carry out large power supersonic dispersion.Preferably, Waters temperature is constant at 25~30 DEG C or so, and ultrasound can get preferable graphene oxide layer peeling effect for 4 hours, is made steady Fixed graphene oxide sol system.

Further, step S4 includes:

It is sub- that sensitizing type optical fiber SPR sensor made from step S3 is put into 1- (3- dimethylamino-propyl) -3- ethyl carbon two In amine hydrochlorate (EDC) and N- hydroxyl amber acid imide (NHS) solution, (4 DEG C) activation 30min of low temperature, taking-up is dried at room temperature.From And the carboxyl (- COOH) of the graphene oxide to sensitizing type optical fiber SPR sensor surface activates.

Further, step S5 includes: to process the monoclonal antibody specific drop coating of tumor markers in step S4 Sensitizing type optical fiber SPR sensor sensitive zones, be placed in 4 DEG C of refrigerators drying and dry, be made a kind of for detecting corresponding mark The Fiber imunosensor 1 of will object has expanded new opplication of 11 sensor of optical fiber in biochemistry detection field.

It,, will using mesothelin as test object for the marker of the tumours such as cancer of pancreas, oophoroma as implementation case column Mesothelin monoclonal antibody is added dropwise in sensitive zones.Carboxyl (- COOH) and Dan Ke after being activated in superficial oxidation graphene film layer Amide reaction occurs for the amino (- NH2) in grand antibody molecule, so that antibody is assembled to be fixed on sensor surface, realizes tumour Real-time, accurate, the high biomolecule identification of specificity.

The above is only the preferred embodiments of the present utility model only, is not intended to limit the utility model, all practical at this Made any modifications, equivalent replacements, and improvements etc., should be included in the guarantor of the utility model within novel spirit and principle Within the scope of shield.

Claims (10)

1. Fiber imunosensor, which is characterized in that including optical fiber, excitation metal film, monoclonal antibody layer and for will be described Monoclonal antibody layer is assembled in the molecular Iy self-assembled layer on the excitation metal film；

The side of the optical fiber is equipped with groove, the fibre core of the bottom wall of the groove adjacent to the optical fiber；

The excitation metal film deposition is on the bottom wall of the groove；

The molecular Iy self-assembled layer is self-assembled on the excitation metal film；

The monoclonal antibody layer is applied on activated treated the molecular Iy self-assembled layer.

2. Fiber imunosensor as described in claim 1, which is characterized in that fibre of the bottom wall of the groove to the optical fiber The distance of core is 1~2 μm.

3. Fiber imunosensor as described in claim 1, which is characterized in that the excitation metal film be golden film or silverskin, It is described excitation metal film with a thickness of 40~50 μm.

4. Fiber imunosensor as claimed in claim 3, which is characterized in that the molecular Iy self-assembled layer is graphene nano Film layer or stannic oxide/graphene nano film layer.

5. Fiber imunosensor as claimed in claim 3, which is characterized in that further include the first articulamentum, described first connects It connects layer to be deposited on the bottom wall of the groove, the excitation metal film deposition is on first articulamentum.

6. Fiber imunosensor as claimed in claim 5, which is characterized in that first articulamentum is chromium film, with a thickness of 2-3nm。

7. Fiber imunosensor as claimed in claim 3, which is characterized in that further include the second articulamentum, described second connects It connects layer to be self-assembled on the excitation metal film, the molecular Iy self-assembled layer is self-assembled on second articulamentum.

8. Fiber imunosensor as claimed in claim 7, which is characterized in that second articulamentum uses with a thickness of nanometer The positive stearylmercaptan of magnitude.

9. Fiber imunosensor as described in claim 1, which is characterized in that the monoclonal antibody layer is by tumor markers Monoclonal antibody constitute.

10. for the detection device of immunity disease detection, including wideband light source, fiber spectrometer and terminal, which is characterized in that It further include such as the described in any item Fiber imunosensors of claim 1-9, the both ends of the Fiber imunosensor lead to respectively It crosses connection optical fiber and connects the wideband light source and the fiber spectrometer, the terminal and the fiber spectrometer communicate to connect.