CN208404966U - A kind of cell transplantation enhancing device - Google Patents
A kind of cell transplantation enhancing device Download PDFInfo
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- CN208404966U CN208404966U CN201720911222.8U CN201720911222U CN208404966U CN 208404966 U CN208404966 U CN 208404966U CN 201720911222 U CN201720911222 U CN 201720911222U CN 208404966 U CN208404966 U CN 208404966U
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Abstract
The utility model belongs to medical transplantation device technical field, more particularly to a kind of cell transplantation enhancing device, gel transplanting block and hydrophobic polyethylene pipe including rectangular-shape, at least one through-hole for running through two opposite side walls is provided on cuboid gel transplanting block, the hydrophobic polyethylene pipe to match with hole size is equipped in through-hole, the length of hydrophobic polyethylene pipe is not less than the length of through-hole.The utility model cell transplantation device can carry any treatment human body cell, be transplanted to subcutaneous, muscle, abdominal cavity, kidney packing and particular treatment position.The utility model transplantation device can promote blood vessel to generate in graft memotron surrounding simultaneously.The utility model transplantation device can increase in right amount or remove graft in migration process, therefore can easy, quick, safely and effectively adjust the dosage of graft.The utility model structure is simple, is easy to make, is able to achieve large-scale production, plays the role for the treatment of related disease.
Description
Technical field
The utility model belongs to medical transplantation device field, is related to a kind of transplantation treatment synergy dress of suitable various kinds of cell
It sets more particularly to islet cell transplantation enhancing device.
Background technique
With the developing and development of medical domain, a kind of novel treatment means are appeared in the visual field of people.Cell moves
The characteristic using certain cells with specific function is planted, amplification in vitro, special training are obtained or passed through using biological engineering method
It supports after waiting processing, has the therapeutic efficiencies such as promotion tissue and organ regeneration, group rehabilitation to achieve the purpose that the certain diseases for the treatment of, this
Method is also increasingly by the concern of scientist.
Nowadays cell transplantation has applied in many diseases, as Autologous Chondrocyte implantation technique repairing articular cartilage damages
Wound, olfactory ensheathing cells transplantation Parkinson's disease, Schwann cell skin grafing and mending spinal cord injury, islet cell transplantation treat diabetes
Deng, wherein the treatment of diabetes is particularly important.Diabetes are a kind of important health problems of facing mankind, and the whole world is about
1.5 hundred million people suffer from diabetes.Whether type 1 diabetes or diabetes B, common trait be due to beta Cell of islet defect or
Missing causes insulin secretion absolutely or relative deficiency, causes sugar, rouge, protein and water, electrolyte metabolism disorders.Drug is controlled
It treats and long term injections exogenous insulin is the primary treatment measure of current diabetes, but these methods can not be solved fundamentally
Certainly diabetic can not prevent the generation of diabetic complication to the Dependence Problem of insulin well.Islet cells moves
It plants and provides better therapy approach for diabetic.Patient receives islet cell transplantation and treats, behind transplanter 1 year of 80%
It is detached from exogenous insulin, while its sugar, amino acid, fat metabolism recovery stabilization, the generation of cardiovascular complications and hair
Exhibition risk gradually decreases.
Although the development that cell transplantation field is making rapid progress, facing most important Railway Project still can not be kept away
Exempt from: 1, immunological rejection is still the main reason for transplanted cells are lost and cause long-term effect poor；2, because anoxic causes to move
Planting cell can not long-term surviving；3, it transplants mostly systemic and cannot sufficiently play a role in related part；4, it transplants
Position is difficult to fix, and graft is difficult to recycle.Therefore, it solves anoxic in cell transplantation procedure, lack nutriment, immunological rejection
Reaction, transplantation site are difficult to fix, and graft is difficult to the committed step that the problems such as recycling is promotion cell transplantation clinical application.
Utility model content
For immunological rejection existing for current cell transplantation, transplantation site lacks oxygen, nutriment, and transplanted cells are difficult to
It is fixed on the problems such as purpose position plays dependent interaction, we develop a kind of novel using high-biocompatibility material as raw material
Cell transplantation synergy protective device is conducive to fixation, long-term surviving, increase and the removal of the transplanting of cell privileged site.For controlling
Related disease is treated, which is also suitble to industrialization production.
In order to realize the purpose of this utility model, the utility model adopts the following technical solution:
A kind of cell transplantation enhancing device, gel transplanting block and hydrophobic polyethylene pipe including rectangular-shape, rectangular
It is provided at least one through-hole for running through two opposite side walls on body gel transplanting block, is equipped in through-hole and is dredged with what hole size matched
Aqueous polyethylene pipe, the length of hydrophobic polyethylene pipe are not less than the length of through-hole.
The cell transplantation enhancing device, transplanting block length is 10-15mm, width 15-20mm, with a thickness of 4-6mm,
Volume is 600-1800mm3.Preferred migration block length is 10mm, width 15mm, with a thickness of 5mm, volume 750mm3。
The cell transplantation enhancing device, through-hole aperture are 0.4-1mm, and preferably through-hole aperture is 0.4mm.
The cell transplantation enhancing device, the gel, which is transplanted on block, microcellular structure.Transplant the microcellular structure of block
Inside contain fibrin gel, the fibrin gel is by adding human fibrinogen, intravascular in CMRL culture medium
It is formed after skin growth factor, epidermal growth factor, fibrin ferment, Pen .- Strep.
The utility model provides that a kind of structure is simple, handling ease, safe operation, cell transplantation device easy to use.
Cell is connected by the blood vessel generated in device with organism, can be transplanted multiple devices simultaneously and be played amplification or collaboration work
With.The device can be transplanted to the different parts such as subcutaneous, abdominal cavity, kidney, and later period replacement increases or removes all very simply easily
Row.
The utility model cell transplantation device can carry any treatment human body cell, be transplanted to subcutaneous, muscle, abdominal cavity,
Kidney packing and particular treatment position.The utility model transplantation device can promote blood vessel in four Zhousheng of graft memotron simultaneously
At, can not only graft be allowed to play a role in transplantation site, secreted by therapeutic substance can also be reached via periphery blood vessel
Whole body plays related curative effect.The utility model cell transplantation device can promote capillary raw around cell storage pipe in advance
At making transplanted cells just obtain sufficient blood supply, oxygen and nutrition in transplanting early stage and survived in vivo to improving transplanted cells
Rate and functional efficiency (it is treated, and sexual secretion can cycle to reach whole body through blood rapidly or disease location plays curative effect).This is practical
Novel transplantation device can increase in right amount or remove graft in migration process, therefore can easy, fast, safely and effectively adjust
Save the dosage of graft.The utility model solves the approach and transplanted sites of histiocytic transplantation treatment, can effectively protect
Transplanted cells are protected, so that it by the attack of immunocyte, is not improved the survival rate of cell, enhances the curative effect of transplanted cells, are reduced
The difficulty and risk of transfer operation, more controllable recycling transplanted cells save time and the expense of operation, ensure that operation
Safety.The utility model structure is simple, is easy to make, is able to achieve large-scale production, plays the role for the treatment of related disease.
Detailed description of the invention
Fig. 1 is the utility model transplantation device structure chart；
Wherein 1 is transplanting block, and 2 be hydrophobic polyethylene pipe；
Fig. 2 is blood glucose in diabetic mice detection after transplanting.
Specific embodiment:
A kind of cell transplantation enhancing device of the utility model, gel transplanting block 1 and hydrophobicity including rectangular-shape are poly-
Ethylene tube 2 is provided at least one through-hole for running through two opposite side walls on cuboid gel transplanting block 1, is equipped with and leads in through-hole
The hydrophobic polyethylene pipe 2 that hole size matches, the length of hydrophobic polyethylene pipe 2 are not less than the length of through-hole.
1 the utility model of embodiment transplants the preparation of enhancing device, uses:
4g poly DL-lactide-caprolactone is dissolved in 50ml chloroform, is added after 20g NaCl is sufficiently stirred and is moved into sterile culture
In ware, transplanting block is obtained after chloroform volatilization is dry in superclean bench, rinses transplanting block with sterile distilled water.Transplanting block casting is made
At 10 × 15 × 5mm, volume 750mm3.It is punched with the hollow iron pipe that aperture is 0.4mm in side face, production graft storage
Pipeline is deposited, is then inserted into graft reservoir conduit with hydrophobic polyethylene pipe.The 2mg people being added in 2ml CMRL culture medium is fine
Fibrillarin original, 50ng vascular endothelial growth factor, 100ng epidermal growth factor, 2ul1% Pen .- Strep and 20ul blood coagulation
Enzyme forms fibrin gel, transplantation device is soaked in fibrin gel, and fibrin gel is made to be solidificated in transplanting dress
In the micropore size set.
An osculum is cut at positions such as subcutaneous or stomach walls, transplantation device is put at nearby bleeding pipe, fixation, is sewed up the incision, etc.
To vascularization in transplantation device, an osculum is cut in same area after 4 weeks, the hydrophobic polyethylene pipe of device is taken out, is
Transplanted cells provide channel, are entered cell infusion in channel with 23G Hamilton syringe.
The transplanting of 2 diabetic mice islet cells of embodiment
First group of diabetic mice enters the utility model transplantation device in its back waist subcutaneous transplantation, after 4 weeks to
In transplantation device after vascularization, a small notch is done above back of mice transplantation device, takes out hydrophobic polyethylene pipe, is pancreas
Island cell provides channel, 1200 islet cells is injected into channel with 23G Hamilton syringe, then with poly- DL- third
Lactide-caprolactone seals access port, sews up the incision.Second group of diabetic mice directly transplanting 1200 under kidney envelope
Islet cells.Third group diabetic mice does not transplant any islet cells as control.
3 diabetic mice blood sugar test of embodiment
After islet cell transplantation, one, two, three group of diabetic mice measures weekly mouse blood sugar level, measures always
Tenth week, third group diabetic mice was as control.Experimental result is shown, carries out islet transplantation with utility model device
After cell, the blood glucose of diabetic mice is decreased obviously, and blood glucose can be always maintained at maintenance level in 10 weeks.Kidney envelope transplantation group
The blood glucose at diabetic mice initial stage is decreased obviously and keeps stable, but later period blood glucose level is stepped up fluctuation and also gradually becomes
Greatly.The blood glucose of non-transplantation group diabetic mice is always maintained at higher blood glucose level, and blood glucose fluctuation is larger.
4 diabetic mice pathological examination of embodiment
After islet cell transplantation, one, two group is put to death for diabetic mice the 11st week, is exempted from respectively to transplantation site
Epidemic disease group insulin dyeing.Experimental result shows, the islet cells in transplantation device nearby has no lymphocytic infiltration, and kidney
Islet cells under envelope has a large amount of lymphocytic infiltration, or even has portion of tissue necrosis, with putting off for transplant time, leaching
The immunization of bar cell, the islet cells under kidney envelope can gradually decrease.This may be that kidney envelope in embodiment 3 is caused to be transplanted
Islet cells mouse late detection to blood glucose level go up and fluctuate a biggish major reason.The experiment results show that this
Utility model transplantation device can preferably protect islet cells to generate barrier action, and the pancreas islet for making immunocyte not attack transplanting is thin
Born of the same parents.
Claims (6)
1. a kind of cell transplantation enhancing device, which is characterized in that gel transplanting block and hydrophobic polyethylene including rectangular-shape
Pipe is provided at least one through-hole for running through two opposite side walls on cuboid gel transplanting block, is equipped in through-hole and hole size
The hydrophobic polyethylene pipe to match, the length of hydrophobic polyethylene pipe are not less than the length of through-hole.
2. cell transplantation enhancing device according to claim 1, which is characterized in that transplanting block length is 10-15mm, width
For 15-20mm, with a thickness of 4-6mm, volume 600-1800mm3。
3. cell transplantation enhancing device according to claim 2, which is characterized in that transplanting block length is 10mm, and width is
15mm, with a thickness of 5mm, volume 750mm3。
4. cell transplantation enhancing device according to claim 1, which is characterized in that through-hole aperture is 0.4-1mm.
5. cell transplantation enhancing device according to claim 4, which is characterized in that through-hole aperture is 0.4mm.
6. cell transplantation enhancing device according to claim 1, which is characterized in that have micropore on the gel transplanting block
Structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720911222.8U CN208404966U (en) | 2017-07-26 | 2017-07-26 | A kind of cell transplantation enhancing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720911222.8U CN208404966U (en) | 2017-07-26 | 2017-07-26 | A kind of cell transplantation enhancing device |
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| Publication Number | Publication Date |
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| CN208404966U true CN208404966U (en) | 2019-01-22 |
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| CN201720911222.8U Active CN208404966U (en) | 2017-07-26 | 2017-07-26 | A kind of cell transplantation enhancing device |
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| Country | Link |
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| CN (1) | CN208404966U (en) |
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2017
- 2017-07-26 CN CN201720911222.8U patent/CN208404966U/en active Active
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