CN208383685U - A kind of Calcium imaging cell perfusion slot - Google Patents
A kind of Calcium imaging cell perfusion slot Download PDFInfo
- Publication number
- CN208383685U CN208383685U CN201820820773.8U CN201820820773U CN208383685U CN 208383685 U CN208383685 U CN 208383685U CN 201820820773 U CN201820820773 U CN 201820820773U CN 208383685 U CN208383685 U CN 208383685U
- Authority
- CN
- China
- Prior art keywords
- coverslip
- groove body
- calcium imaging
- placing groove
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses a kind of Calcium imaging cell perfusion slots, including groove body and slot cover, groove body is equipped with coverslip placing groove, the loophole for calcium imaging excitation-light unit irradiating cell creep plate is equipped in the middle part of the bottom surface of coverslip placing groove, the both ends of groove body are equipped with inlet opening, slot cover includes the lug boss in coverslip placing groove, the middle part of lug boss is equipped with creep plate peep hole, several feed pathways of connection creep plate peep hole and each inlet opening are additionally provided in lug boss, it is additionally provided with fluid cushion pond, first coverslip is placed in coverslip placing groove when use and seals loophole, then cell climbing sheet is placed on the coverslip above loophole, slot cover is covered tightly again, lug boss packed cell creep plate, the liquid of perfusion is first stored in fluid cushion pond, then slowly flow into creep plate peep hole, it avoids directly impacting cell climbing sheet, To reduce the damage to cell, this utility model is used for Bioexperiment clothing arts.
Description
Technical field
The utility model relates to Bioexperiment clothing arts, more particularly to a kind of Calcium imaging cell perfusion slot.
Background technique
Calcium ion imaging technology carries out fluorescent staining to cell with Fura-2.Cell Fura- on cell climbing sheet
After 2 dyeing, usually cell and creep plate are fixed in a cell perfusion slot, it is aobvious by the way that cell perfusion slot is placed in calcium imaging
On the objective table of micro mirror, feed pathway then is connected in perfusion slot both ends and liquid outlet channel gives different pharmaceutical perfusion, with observation
Effect of the drug for calcium channel.
When existing cell perfusion slot perfusion, it need to be fixed by bolts cell climbing sheet, since perfusion slot is under dark room conditions
Operation, the fixed cell climbing sheet of the small screw of Reusability, increases operation difficulty degree, greatly extends experiment duration, fills
The medical fluid of stream can usually be directly impinging cell climbing sheet, cause direct losses to cell, influence the good state of cell and reality
Test result.
Utility model content
A kind of the purpose of this utility model is to provide perfusions easy to operate, small to cellular damage Calcium imaging cell
Perfusion slot.
Technical solution adopted in the utility model is:
A kind of Calcium imaging cell perfusion slot, including groove body and the slot cover being detachably connected with groove body, groove body is equipped with
Coverslip placing groove, the bottom surface middle part of coverslip placing groove are equipped with the light transmission for calcium imaging excitation-light unit irradiating cell creep plate
Hole, the both ends of groove body are equipped with the inlet opening communicated with coverslip placing groove, and slot cover includes convex in coverslip placing groove
The portion of rising, the middle part of lug boss are equipped with creep plate peep hole corresponding with light transmission hole site, and connection creep plate observation is additionally provided in lug boss
Several feed pathways in hole and each inlet opening, are additionally provided with fluid cushion pond between each feed pathway and creep plate peep hole.
It is further used as the improvement of technical solutions of the utility model, the two sides of slot cover are equipped with the wing plate extended downwardly, slot
Buckle is equipped on the inside of the wing plate of lid two sides, the two relative side of groove body is equipped with and buckles mutually matched card slot.
It is further used as the improvement of technical solutions of the utility model, the two relative side of groove body, which is equipped with, is placed in microscope load
The placement plate of object platform two sides.
It is further used as the improvement of technical solutions of the utility model, the bottom of groove body is equipped with convenient for lens focusing around saturating
The circular trough of unthreaded hole.
It is further used as the improvement of technical solutions of the utility model, two fluid cushion ponds are symmetrically disposed on creep plate peep hole
Two sides, regardless of anyway, symmetrical fool-proof design, i.e., when in use, due to fluid cushion pond symmetric design, user is without dividing
Distinguish the obverse and reverse of groove body, thus device damage caused by capable of preventing user when in use due to positive and negative resolution is wrong or
Nuisance operation.Using the symmetrical fool-proof design in fluid cushion pond, allow operator do not need to spend attention, do not need yet experience with
Professional knowledge can the errorless completion of intuition correctly operation.
It is further used as the improvement of technical solutions of the utility model, one end of inlet opening is tilted towards coverslip placing groove
Bottom surface.
The utility model has the beneficial effects that this Calcium imaging cell perfusion slot, coverslip is first placed in lid glass by when use
In piece placing groove and loophole is sealed, then cell climbing sheet is placed on the coverslip above loophole, then by the convex of slot cover
It in the portion of rising insertion coverslip placing groove and covers tightly, lug boss packed cell creep plate, perfusion can be carried out at this time, the liquid of perfusion is logical
Crossing inlet opening has fluid passage to flow into and is first stored in fluid cushion pond, then slowly flows into creep plate peep hole, avoids straight
Impact cell climbing sheet is connect, so that the damage to cell is reduced, fluid cushion of the liquid through the creep plate peep hole other side after perfusion
Pond, fluid passage and inlet opening outflow, the both ends of lug boss perfusion structure having the same, so that slot cover is not necessarily to point during installation
It distinguishes front and back end, improves the convenience of operation.
Detailed description of the invention
The utility model is described in further detail with reference to the accompanying drawing:
Fig. 1 is the overall structure diagram of the utility model embodiment；
Fig. 2 is the schematic diagram of internal structure of the utility model embodiment；
Fig. 3 is the schematic diagram of the groove body of the utility model embodiment and the faying face of slot cover；
Fig. 4 is the structural schematic diagram of the groove body of the utility model embodiment.
Specific embodiment
Referring to Fig.1~Fig. 4, the utility model be a kind of Calcium imaging cell perfusion slot, including groove body 1 and with groove body 1
The slot cover 2 being detachably connected, groove body 1 are equipped with coverslip placing groove 11, are equipped in the middle part of the bottom surface of coverslip placing groove 11 for calcium
The loophole 12 of excitation-light unit irradiating cell creep plate is imaged, the both ends of groove body 1, which are equipped with, to be communicated with coverslip placing groove 11
Inlet opening 13, slot cover 2 include the lug boss 21 in coverslip placing groove 11, and the middle part of lug boss 21 is equipped with and loophole
The corresponding creep plate peep hole 22 in 12 positions, be additionally provided in lug boss 21 connection creep plate peep hole 22 and each inlet opening 13 it is several into
Liquid channel 24 is additionally provided with fluid cushion pond 23 between each feed pathway 24 and creep plate peep hole 22.
Coverslip is first placed in coverslip placing groove 11 to when use and is sealed loophole 12, then places cell climbing sheet
It is embedded in coverslip placing groove 11 and covers tightly, lug boss on the coverslip above loophole 12, then by the lug boss 21 of slot cover 2
21 packed cell creep plates, can carry out perfusion at this time, and the liquid of perfusion has fluid passage to flow into and first stores by inlet opening 13
It in fluid cushion pond 23, then slowly flows into creep plate peep hole 22, avoids directly impacting cell climbing sheet, to reduce to thin
The damage of born of the same parents, fluid cushion pond 23, fluid passage and inlet opening 13 of the liquid through 22 other side of creep plate peep hole after perfusion flow
Out, the both ends of lug boss 21 perfusion structure having the same, so that slot cover 2 improves behaviour during installation without differentiating front and back end
The convenience of work.
As the utility model preferred embodiment, the two sides of slot cover 2 are equipped with the wing plate 25 extended downwardly, each wing plate
25 inside is equipped with buckle, and the two relative side of groove body 1 is equipped with and buckles mutually matched card slot.
Since Fura-2 fluorescence is easily quenched under light environment, therefore would generally when cell climbing sheet is fixed on perfusion slot
It is carried out in darkroom.Therefore, in installation and replacement cell climbing sheet, the degree convenient for disassembly and assembly of groove body 1 and slot cover 2 will be determined directly carefully
The disassembly efficiency of born of the same parents' creep plate.The utility model devises easy-to-mount buckle and card slot, so that operator also can in darkroom
Groove body 1 and slot cover 2 are easily dismounted very much, to be quickly installed cell climbing sheet.
As the utility model preferred embodiment, the two relative side of groove body 1, which is equipped with, is placed in microscope carrier
The placement plate 15 of two sides.
As the utility model preferred embodiment, the bottom of groove body 1 is equipped with the circular loophole convenient for lens focusing
12 circular trough 14.
As the utility model preferred embodiment, fluid cushion pond 23 is in rectangle, and two fluid cushion ponds 23 are symmetrically set
It is placed in the two sides of creep plate peep hole 22.
As the utility model preferred embodiment, one end of inlet opening 13 is tilted towards coverslip placing groove 11
Bottom surface.
Certainly, the invention is not limited to above embodiment, and those skilled in the art are without prejudice to originally
Equivalent deformation or replacement can be also made under the premise of utility model spirit, these equivalent variation or replacement are all contained in the application
In claim limited range.
Claims (6)
1. a kind of Calcium imaging cell perfusion slot, it is characterised in that: detachably connect including groove body (1) and with the groove body (1)
The slot cover (2) connect, the groove body (1) are equipped with coverslip placing groove (11), in the middle part of the bottom surface of the coverslip placing groove (11)
Equipped with for calcium imaging excitation-light unit irradiating cell creep plate loophole (12), the both ends of the groove body (1) be equipped with it is described
The inlet opening (13) that coverslip placing groove (11) communicates, the slot cover (2) include in the coverslip placing groove (11)
The middle part of lug boss (21), the lug boss (21) is equipped with creep plate peep hole (22) corresponding with the loophole (12) position,
Several feed pathways (24) of the connection creep plate peep hole (22) and each inlet opening (13) are additionally provided in the lug boss (21),
Fluid cushion pond (23) are additionally provided between each feed pathway (24) and creep plate peep hole (22).
2. Calcium imaging cell perfusion slot according to claim 1, it is characterised in that: the two sides of the slot cover (2) are equal
Equipped with the wing plate (25) extended downwardly, it is equipped with buckle on the inside of the wing plate (25) of slot cover (2) two sides, the two of the groove body (1)
Opposite flank is equipped with and the mutually matched card slot of buckle.
3. Calcium imaging cell perfusion slot according to claim 2, it is characterised in that: the two of the groove body (1) are opposite
Side is equipped with the placement plate (15) for being placed in microscope carrier two sides.
4. Calcium imaging cell perfusion slot according to claim 1, it is characterised in that: the bottom of the groove body (1) is set
There is the circular trough (14) around the loophole (12) convenient for lens focusing.
5. Calcium imaging cell perfusion slot according to claim 1, it is characterised in that: the fluid cushion pond (23) is in
Rectangle, the two fluid cushion ponds (23) are symmetrically disposed on the two sides of the creep plate peep hole (22).
6. Calcium imaging cell perfusion slot according to claim 1, it is characterised in that: one end of the inlet opening (13)
It tilts towards the bottom surface of the coverslip placing groove (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820820773.8U CN208383685U (en) | 2018-05-29 | 2018-05-29 | A kind of Calcium imaging cell perfusion slot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820820773.8U CN208383685U (en) | 2018-05-29 | 2018-05-29 | A kind of Calcium imaging cell perfusion slot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208383685U true CN208383685U (en) | 2019-01-15 |
Family
ID=64969467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820820773.8U Expired - Fee Related CN208383685U (en) | 2018-05-29 | 2018-05-29 | A kind of Calcium imaging cell perfusion slot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208383685U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175298A (en) * | 2019-11-22 | 2020-05-19 | 浙江农林大学 | Sweet taste solution detection device and method |
-
2018
- 2018-05-29 CN CN201820820773.8U patent/CN208383685U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111175298A (en) * | 2019-11-22 | 2020-05-19 | 浙江农林大学 | Sweet taste solution detection device and method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190115 Termination date: 20210529 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |