CN208204719U - A kind of irradiation unit filling bar for observing embryo under liquid nitrogen - Google Patents

A kind of irradiation unit filling bar for observing embryo under liquid nitrogen Download PDF

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Publication number
CN208204719U
CN208204719U CN201820865241.6U CN201820865241U CN208204719U CN 208204719 U CN208204719 U CN 208204719U CN 201820865241 U CN201820865241 U CN 201820865241U CN 208204719 U CN208204719 U CN 208204719U
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China
Prior art keywords
liquid nitrogen
irradiation
mist
lamp
cap body
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CN201820865241.6U
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Chinese (zh)
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饶金鹏
金敏
封纯
邱枫
蔡益婷
钱小红
金帆
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

A kind of irradiation unit filling bar for observing embryo under liquid nitrogen, the cap body including that can be fixed on user head, the cap body includes first half and latter half;The lower section of the first half is equipped with the goggles for blocking user's eyes, and the goggles can be rotated by the first rotating device and the cap of the first half can positioningly be arranged in along upper;The upper surface of the cap body is equipped with spotlighting portion and wears mist irradiation portion, and the spotlighting portion and the mist irradiation light portion of wearing are respectively positioned on the first half.Lamp group and spotlighting lamp group common irradiation are irradiated using mist is worn, it can be in the diffraction for guaranteeing to greatly reduce light while brightness, namely it effectively reduces " Tyndall effect " and is adversely affected caused by observation, more effectively luminous energy are allowed to penetrate thick and heavy nitrogen vapor and enter liquid nitrogen, so that experimenter embryo can fill bar under clear, comfortable observation liquid nitrogen, the accuracy of observation result is also ensured.

Description

A kind of irradiation unit filling bar for observing embryo under liquid nitrogen
Technical field
The utility model relates to medical instruments field more particularly to a kind of irradiation dresses that bar is filled for observing embryo under liquid nitrogen It sets.
Background technique
With the relieving of national " two children " policy comprehensively, more and more women have regeneration to educate demand.But since the age increases Greatly, environmental pollution, job and life stress be big and the overlaying influence of the factors such as bad life style, the current every 7 couples of Mr. and Mrs in China In there are about 1 pair can not carry out normal reproduction, this would have to by supplementary reproduction (test-tube baby) technology.
And when embryo experiments room carries out IVF-ET (in vitro fertilization-embryo transfer) operation, often it will appear using embryo Situations such as tire number is not in the best embryo transfer phase greater than transplanting embryo number or wife's side endometrium, at this time just must be to embryo Tire carries out freezen protective operation.
Embryo had to pass through before carrying out long-term frozen preservation dress bar the step of, and the step for overall process must be in liquid It completes, but liquid nitrogen has extremely strong volatility, is understood in the fog of liquid nitrogen surface formation one thickness weight under state nitrogen, and liquid nitrogen and air Belong to different media again, light, which enters liquid nitrogen, can occur refraction effect, and embryo experiments room integrated environment is partially dark in addition, so that seeing It is very difficult to examine embryo's dress bar under liquid nitrogen.
In order to which transmission nitrogen vapor can preferably observe the dress bar position of embryo under liquid nitrogen to guarantee the dress bar effect of embryo, Laboratory operation personnel often by head as much as possible close to liquid nitrogen liquid level, while as far as possible shorten carry bar reach liquid level away from From, but this there is because observe it is unclear due to do not control relative distance make carry bar emersion liquid nitrogen liquid level risk, and once plus The load bar for having carried embryo will cause the injury of lethal departing from liquid nitrogen to embryo;Meanwhile lab assistant is apart from liquid nitrogen mistake Closely, the liquid nitrogen volatilized can be sucked, once and liquid nitrogen sputter come, easily the eyes of personnel and skin are damaged.
Therefore, experimenter visually observes under liquid nitrogen embryo and fills bar and exist and do not see, is easy to cause load bar in the prior art Emersion liquid nitrogen liquid level and haves the defects that human injury to experimenter.
Summary of the invention
It visually observes under liquid nitrogen embryo and fills bar and exist to solve experimenter in the prior art and do not see, be easy to cause load Bar emersion liquid nitrogen liquid level and there are problems that human injury to experimenter, the utility model provides a kind of irradiation and penetrates effect Good, easy to use and flexible and the irradiation unit that bar is filled for observing embryo under liquid nitrogen for having both safeguard function.
The technical solution that the utility model solves the above problems is:
Embodiments herein provide it is a kind of fill the irradiation unit of bar for observing embryo under liquid nitrogen, including can be fixed on and make The cap body on user head, the cap body include first half and latter half;The lower section of the first half is equipped with for blocking The goggles of user's eyes, and the goggles can be rotated by the first rotating device and the first half can positioningly be arranged in Partial cap is along upper;
The upper surface of the cap body is equipped with spotlighting portion and wears mist irradiation portion, and the spotlighting portion and described wears Mist irradiation light portion is respectively positioned on the first half.
Further, the spotlighting portion includes two spotlighting lamp groups, and the mist irradiation portion of wearing is worn including one Mist irradiates lamp group;The centre worn mist irradiation lamp group and be located at the cap body first half, two spotlight illumination lamp group difference The left and right sides for wearing mist irradiation lamp group is set, and two spotlighting lamp groups are respectively positioned on the mist of wearing and irradiate lamp group Rear.
Further, the spotlighting lamp group and the mist irradiation lamp group of wearing include frame body, the bottom end of the frame body It is fixed on the upper surface of the cap body, the top of the frame body extends straight up and connect with lamp holder;The lamp holder it is interior End can be rotated and can be positioningly arranged on the frame body by the second rotating device, and the outer end of the lamp holder is to lamp holder extension It stretches, several LED lamp beads is equipped in the lamp holder;The LED lamp bead passes through conducting wire and is connected with power supply, and the power supply Power supply is equipped with switch.
Further, the LED lamp bead being located at there are three being set in the lamp holder on three angles of isosceles triangle.
Further, the wavelength of the light of the LED lamp bead transmitting of the spotlighting lamp group is 450nm~460nm;It is described The wavelength for wearing the light of the LED lamp bead transmitting of mist irradiation lamp group is 600nm~620nm.
Further, first rotating device includes the first connector, and the top of first connector is fixed on institute The cap of first half is stated along upper, the bottom end of first connector extends straight down and is fixedly linked with the top surface of shaft;Institute It is horizontally disposed and perpendicular with first connector to state shaft;The top of the goggles is equipped with the second connector, and described the The bottom end of two connectors is connected with the goggles, and the top of second connector, which is equipped with, is rotationally fastened on the shaft On body.
Further, second rotating device includes the ball groove being arranged at the top of the frame body and is rotationally fastened on Bulb in the ball groove is provided with through-hole in the ball groove, and one end of third connector is fixedly linked with the bulb, and third connects The other end of fitting is fixedly linked through the through-hole and with the inner end of lamp holder.
Further, the left side two sides of the cap body are respectively provided with the first bandage and that cap body is fixed on to head Two bandages, and the first bandage and the second bandage are detachably connected by velcro.
The beneficial effects of the utility model are mainly manifested in:
1. user fills bar with embryo under liquid nitrogen is observed after uppercap body, before goggles is placed exactly in the eyes of user just The risk of liquid nitrogen splashing injury eyes is effectively evaded, to protect user's to use eye to stop liquid nitrogen to fly into eyes in side Safety, and the goggles can be overturn by the first rotating device does not need especially to facilitate in use, may be reversed up Observation when above the round-trip microscope of lab assistant and liquid nitrogen box to embryo.
2, fog lamp (wearing mist irradiation lamp group) cooperation left and right sides white spotlight (i.e. optically focused photograph is worn using preposition orange light Bright lamp group) common irradiation come assistant experiment personnel observe liquid nitrogen under embryo fill bar, can be greatly reduced while guaranteeing brightness The diffraction of light, namely effectively reduce " Tyndall effect " and adversely affected caused by observation, allow more effectively luminous energy to penetrate thickness The nitrogen vapor of weight and enter liquid nitrogen so that experimenter can embryo's dress bar under clear, comfortable observation liquid nitrogen, also ensure sight Examine the accuracy of result.
3, experimenter does not need to pass through shortening by mist irradiation lamp group and i.e. spotlighting lamp group observation sufficiently clear is worn It carries bar and reaches the distance of liquid level to observe, avoid the risk for carrying bar emersion liquid nitrogen liquid level, also ensure the safety of embryo.
4, it wears the lamp holder of mist irradiation lamp group and i.e. spotlighting lamp group and angular adjustment is carried out by the second rotating device, just In to the embryo of different depth position under the liquid nitrogen and carrying bar and clearly observed.
5, the first bandage and the second bandage can be bonded at chin by velcro, to fastening cap body on head; Meanwhile velcro is convenient for adjusting elasticity, and the adjusting of any elasticity, adaptation can be carried out for the head dummy of different experiments room personnel Different experiments personnel, and all convenient, suitable laboratory use is unclamped or cemented to velcro.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for filling the irradiation unit of bar for observing embryo under liquid nitrogen in an embodiment;
Fig. 2 is the structure top view that mist irradiation lamp group is worn in an embodiment.
Specific embodiment
It is clearly and completely described below in conjunction with technical solution of the attached drawing to the utility model patent, it is clear that retouched The embodiment stated is the utility model a part of the embodiment, instead of all the embodiments.Based on the implementation in the utility model Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to The range of the utility model protection.
In the description of the present invention, it should be noted that such as occur term " center ", "upper", "lower", " left side ", The orientation or positional relationship of the instructions such as " right side ", "vertical", "horizontal", "inner", "outside" is orientation based on the figure or position Relationship is merely for convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning must There must be specific orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.This Outside, such as there is term " first ", " second ", " third " are used for description purposes only, be not understood to indicate or imply relatively heavy The property wanted.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, such as there is term " installation ", " connected ", " connection " shall be understood in a broad sense, for example, it may be fixedly connected, may be a detachable connection or one Connect to body;It can be mechanical connection, be also possible to be electrically connected;It can be directly connected, it can also be indirect by intermediary It is connected, can be the connection inside two elements.For the ordinary skill in the art, on being understood with concrete condition State the concrete meaning of term in the present invention.
Referring to Figures 1 and 2, the present embodiment provides a kind of irradiation units that bar is filled for observing embryo under liquid nitrogen, including can It is fixed on the cap body 1 on user head, the cap body 1 includes first half and latter half;It is set below the first half There is the goggles 9 for blocking user's eyes, and the goggles 9 can be rotated and can positioningly be set by the first rotating device The cap in the first half is set along upper;
Specifically, the cap body 1 is in hollow hemisphere shape;Transparent sheet glass, plastic sheet or tree can be used in the goggles 9 Rouge piece etc., which neither blocks sight again, can play the role of protecting the sheet body of eyes, and goggles 9 can be rectangular sheet, if in order to adapt to people Body facial contours, goggles 9 can also be along the curved arc-like sheet in facial width direction.
Wherein, the first half refers to being worn over a part of cap body 1 behind head close to forehead, and the latter half refers to Be after being worn over head cap body 1 close to a part of the back side of head.
Embryo under liquid nitrogen is observed after user's (generally lab assistant) band uppercap body 1 and fills bar, and goggles 9 is placed exactly in Immediately ahead of the eyes of user, to stop liquid nitrogen to fly into eyes, effectively evade the risk of liquid nitrogen splashing injury eyes, thus Protect user's to use eye safety;And the goggles 9 can be overturn by the first rotating device, not needed in use, i.e. Observation when especially facilitating above the round-trip microscope of lab assistant and liquid nitrogen box up to embryo may be reversed.
The upper surface of the cap body 1 is equipped with spotlighting portion and wears mist irradiation portion, and the spotlighting portion and described Mist irradiation light portion is worn to be respectively positioned on the first half.
Specifically, the spotlighting lamp group 4 emits white light, to provide illumination;The mist irradiation lamp group 3 of wearing emits Orange light, to reduce the diffraction of light.
Further, the spotlighting portion includes two spotlighting lamp groups 4, and the mist irradiation portion of wearing is worn including one Mist irradiates lamp group 3;The centre worn mist irradiation lamp group 3 and be located at 1 first half of cap body, two spotlighting lamp groups 4 It is separately positioned on the left and right sides for wearing mist irradiation lamp group 3, and two spotlighting lamp groups 4 are respectively positioned on the mist of wearing and shine The rear of shot-light group 3.
Specifically, be biased to user's left hand side is left after the left and right sides refers to that cap body 1 is worn over user head Side, be biased to user's right hand side is right side.
Further, the spotlighting lamp group and the mist irradiation lamp group of wearing include frame body 2, the bottom of the frame body 2 End is fixed on the upper surface of the cap body 1, and the top of the frame body 2 extends straight up and connect with lamp holder;The lamp holder Inner end can be rotated and can positioningly be arranged on the frame body 2 by the second rotating device, the outer end of the lamp holder is to lamp holder Outer extension, the lamp holder is interior to be equipped with several LED lamp beads 31,41, and the LED lamp bead 31,41 is electric by conducting wire 7 and power supply Source 5 is connected;The power supply 5 is furnished with switch 6.
Specifically, parallel with one another between each lamp holder, each LED lamp bead 31,41 in each lamp holder is parallel with one another.
Wherein, the latter half (dead astern of entire cap body 1) of the cap body 1 is arranged in the power supply 5, and The power supply 5 is furnished with battery case.The conducting wire 7 of each LED lamp bead 31,41 in each lamp holder sequentially passes through the lamp where it It is connected after in seat, the second rotating device where it and cap body 1 with power supply 5.
In the present embodiment, the lamp holder can carry out angular adjustment by the second rotating device, convenient under liquid nitrogen The embryo of different depth position and load bar are clearly observed.
Further, set in the lamp holder there are three be located at LED lamp bead 31 on three angles of isosceles triangle, 41。
Specifically, LED lamp bead 31,41, which is located on three angles of isosceles triangle, is conducive to light convergence.
Further, the wavelength for the light that the LED lamp bead 41 of the spotlighting lamp group 4 emits is 450nm~460nm; The wavelength for the light that the LED lamp bead 31 for wearing mist irradiation lamp group 3 emits is 600nm~620nm.
Specifically, the light that the LED lamp bead 41 of the spotlighting lamp group 4 emits be white, and wavelength be 450nm~ 460nm;The light that the LED lamp bead 31 for wearing mist irradiation lamp group 3 emits is orange, and wavelength is 600nm~620nm.
The present embodiment wears fog lamp (wearing mist irradiation lamp group 3) cooperation left and right sides white spotlight using preposition orange light (i.e. spotlighting lamp group 4) common irradiation carrys out assistant experiment personnel and observes embryo's dress bar under liquid nitrogen, can guarantee the same of brightness When greatly reduces the diffraction of light, and (Experiments of Optics as the result is shown: in all coloured light, wavelength is the orange of 600nm~620nm The penetration capacity of light is only second to red light, but observing effect and excellent comfort in red light), namely effectively reduce " dindar Effect " is adversely affected caused by observation, allows more effectively luminous energy to penetrate thick and heavy nitrogen vapor and enter liquid nitrogen, so that real The person of testing embryo can fill bar under clear, comfortable observation liquid nitrogen, also ensure the accuracy of observation result;Experimenter's observation is clear enough Chu does not need the distance by shortening load bar arrival liquid level to observe, avoids the risk for carrying bar emersion liquid nitrogen liquid level, guarantee yet The safety of embryo.
Wherein, " Tyndall effect " refers to penetrating colloid when Ray Of Light, is observed that colloid from incident light direction In one bright " access " occurring, this phenomenon is also Tyndall effect (Tyndall effect) Tyndall phenomenon Or Ding Zeer phenomenon, Ding Zeer effect, Ting get Er effect.
Further, first rotating device includes the first connector, and the top of first connector is fixed on institute The cap of first half is stated along upper, the bottom end of first connector extends straight down and is fixedly linked with the top surface of shaft 8;Institute It is horizontally disposed and perpendicular with first connector to state shaft 8;The top of the goggles 9 is equipped with the second connector, described The bottom end of second connector is connected with the goggles 9, and the top of second connector is described equipped with being rotationally fastened on Body 91 in shaft 8.
Specifically, the body 91 can be rotated and be positioned around the shaft 8, when experimenter needs using goggles 9 When, goggles 9 is downwardly turned over to drawing axis, when experimenter does not need using goggles 9, by goggles 9 to turning over 1 front of cap body is gone to, since body 91 is fastened in shaft 8, body 91 turns to unspecified angle and can all be located in shaft 8. There is the space overturn for goggles 9 below the cap body 1.
Further, second rotating device includes the ball groove 21 being arranged at the top of the frame body and rotationally card is set Bulb 32,42 in the ball groove is provided with through-hole, one end of third connector and the bulb 32,42 in the ball groove 21 It is fixedly linked, the other end of third connector is fixedly linked through the through-hole and with the inner end of lamp holder.
Specifically, the bulb 32,42 can rotate in ball groove 21, thus the tilt angle of adjustable lamp holder, bulb 32, 42 are fastened in ball groove 21, and bulb 32,42 turns to unspecified angle and can all be located in ball groove 21.
Wherein, it is equipped with first passage in the third connector and lamp holder, is equipped with second channel in the bulb 32,42, Be equipped with third channel in the frame body 2, be equipped with fourth lane in the cap body 1, first passage, second channel, third channel and Fourth lane successively penetrates through composition for the perforative access of conducting wire 7 of each LED lamp bead 31,41.7 long enough of conducting wire, to allow ball First 32,42 will not involve conducting wire 7 when rotating in ball groove 21.
First through hole 321,421 is offered on the bulb 32,42, and the second through-hole 211, institute are offered in the ball groove 21 It states second channel and is connected by the first through hole 321 of perforation with second through-hole 211 with the third channel.
Further, the left side two sides of the cap body 1 are respectively provided with the first bandage 10 that cap body 1 is fixed on to head With the second bandage, and the first bandage 10 and the second bandage are detachably connected by velcro.
Specifically, the top of the first bandage 10 and the top of the second bandage can be fixed on 1 inner wall of cap body, the first bandage 10 Bottom end and the second bandage bottom end it is free.The inner surface of first bandage 10 is equipped with the flannelette of velcro, the second bandage it is outer Surface is equipped with the hook surface of velcro, and the first bandage 10 and the second bandage can be bonded at chin by velcro, thus by cap Body 1 is fastened on head;Meanwhile velcro is convenient for adjusting elasticity, can carry out for the head dummy of different experiments room personnel any The adjusting of elasticity is adapted to different experiments personnel, and all convenient, suitable laboratory use is unclamped or cemented to velcro.
It is as follows for observing the application method for the irradiation unit that embryo fills bar under liquid nitrogen described in the present embodiment:
(1) cap body 1 is worn on head, and the first bandage 10 and the second bandage is bonded at chin by velcro, To which cap body 1 is fixed on head.
(2) switch 6 for opening power supply 5 rotates the lamp holder of spotlighting lamp group 4 and wears the lamp holder of mist irradiation lamp group 3, To adjust the radiation direction of spotlighting lamp group 4 according to the specifically used demand of user and wear the light side of mist irradiation lamp group 3 To.
(3) goggles 9 is downwardly turned over to drawing axis to and regulated the tilt angle of goggles 9, can start to observe Embryo fills bar under liquid nitrogen.
Content described in this specification embodiment is only an enumeration of the implementation forms of the utility model concept, this is practical new The protection scope of type should not be construed as being limited to the specific forms stated in the embodiments, and the protection scope of the utility model is also wrapped Include those skilled in the art according to the utility model design it is conceivable that equivalent technologies mean.

Claims (8)

1. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen, it is characterised in that: including user head can be fixed on Cap body, the cap body includes first half and latter half;The lower section of the first half is equipped with double for blocking user The goggles of eye, and the goggles can be rotated by the first rotating device and the cap of the first half can positioningly be arranged in Along upper;
The upper surface of the cap body is equipped with spotlighting portion and wears mist irradiation portion, and the spotlighting portion and the mist of wearing shine Shot-light portion is respectively positioned on the first half.
2. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as described in claim 1, it is characterised in that: described poly- Optical illumination portion include two spotlighting lamp groups, it is described wear mist irradiation portion include one wear mist irradiation lamp group;It is described to wear mist irradiation Lamp group is located at the centre of the cap body first half, and two spotlight illumination lamp groups are separately positioned on the mist of wearing and irradiate lamp group The left and right sides, and two spotlighting lamp groups are respectively positioned on the rear for wearing mist irradiation lamp group.
3. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as claimed in claim 2, it is characterised in that: described poly- Optical illumination lamp group and the mist irradiation lamp group of wearing include frame body, and the bottom end of the frame body is fixed on the upper surface of the cap body On, the top of the frame body extends straight up and connect with lamp holder;The inner end of the lamp holder can be turned by the second rotating device It moves and can positioningly be arranged on the frame body, the outer end of the lamp holder extends to outside lamp holder, is equipped with several in the lamp holder LED lamp bead;The LED lamp bead passes through conducting wire and is connected with power supply, and the power supply is equipped with switch.
4. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as claimed in claim 3, it is characterised in that: the lamp The LED lamp bead being located at there are three being set in seat on three angles of isosceles triangle.
5. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as claimed in claim 3, it is characterised in that: described poly- The wavelength of the light of the LED lamp bead transmitting of optical illumination lamp group is 450nm~460nm;The LED lamp bead hair for wearing mist irradiation lamp group The wavelength for the light penetrated is 600nm~620nm.
6. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as described in claim 1, it is characterised in that: described the One rotating device includes the first connector, and the top of first connector is fixed on the cap of the first half along upper, described The bottom end of first connector extends straight down and is fixedly linked with the top surface of shaft;The shaft is horizontally disposed and with described the A connection piece is perpendicular;The top of the goggles is equipped with the second connector, the bottom end of second connector and the eye protection Mirror is connected, and the top of second connector is equipped with the body being rotationally fastened in the shaft.
7. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as claimed in claim 3, it is characterised in that: described the Two rotating devices include the bulb that the ball groove at the top of the frame body is arranged in and is rotationally fastened in the ball groove, the ball Through-hole is provided on slot, one end of third connector is fixedly linked with the bulb, and the other end of third connector is through described logical The inner end of Kong Bingyu lamp holder is fixedly linked.
8. a kind of irradiation unit for filling bar for observing embryo under liquid nitrogen as described in claim 1, it is characterised in that: the cap The left side two sides of body are respectively provided with the first bandage and the second bandage that cap body is fixed on to head, and the first bandage and second Bandage is detachably connected by velcro.
CN201820865241.6U 2018-05-31 2018-05-31 A kind of irradiation unit filling bar for observing embryo under liquid nitrogen Active CN208204719U (en)

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CN201820865241.6U CN208204719U (en) 2018-05-31 2018-05-31 A kind of irradiation unit filling bar for observing embryo under liquid nitrogen

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Application Number Priority Date Filing Date Title
CN201820865241.6U CN208204719U (en) 2018-05-31 2018-05-31 A kind of irradiation unit filling bar for observing embryo under liquid nitrogen

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108626630A (en) * 2018-05-31 2018-10-09 浙江大学 A kind of irradiation unit filling bar for observing embryo under liquid nitrogen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108626630A (en) * 2018-05-31 2018-10-09 浙江大学 A kind of irradiation unit filling bar for observing embryo under liquid nitrogen

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