CN208187975U - A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria - Google Patents
A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria Download PDFInfo
- Publication number
- CN208187975U CN208187975U CN201820529162.8U CN201820529162U CN208187975U CN 208187975 U CN208187975 U CN 208187975U CN 201820529162 U CN201820529162 U CN 201820529162U CN 208187975 U CN208187975 U CN 208187975U
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- Prior art keywords
- constant temperature
- sample
- detection unit
- temperature sleeve
- positioning housing
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- 238000004458 analytical method Methods 0.000 title claims abstract description 19
- 230000001766 physiological effect Effects 0.000 title claims abstract description 12
- 210000004027 cell Anatomy 0.000 title claims abstract description 11
- 210000003470 mitochondria Anatomy 0.000 title claims abstract description 10
- 238000001514 detection method Methods 0.000 claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 16
- 239000001301 oxygen Substances 0.000 claims abstract description 16
- 230000003287 optical effect Effects 0.000 claims description 27
- 230000005540 biological transmission Effects 0.000 claims description 24
- 230000001413 cellular effect Effects 0.000 claims description 2
- 238000010276 construction Methods 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 1
- 230000001360 synchronised effect Effects 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 229910000838 Al alloy Inorganic materials 0.000 description 2
- 239000004696 Poly ether ether ketone Substances 0.000 description 2
- 229910001069 Ti alloy Inorganic materials 0.000 description 2
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920002530 polyetherether ketone Polymers 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a kind of cells and a variety of physiological activity index Synchronization Analysis devices of mitochondria, including sample detection unit, reference detection unit, ultraviolet specrophotometer, first data acquisition circuit and the second data acquisition circuit, sample detection unit includes constant temperature sleeve, positioning housing, sample container, oxygen electrode and blender, constant temperature sleeve is hollow cube, outer wall is equipped with baffle, pipeline is equipped in constant temperature sleeve, positioning housing is equipped with protrusion, protrusion is the cavity of upper end opening, the lug boss of positioning housing gos deep into the hollow position of constant temperature sleeve from constant temperature sleeve bottom, the bottom of positioning housing is successively equipped with blender from bottom to top, oxygen electrode and sample container, the upper end of positioning housing protrusion is successively fitted with cock from bottom to top, adjust lock and water conservancy diversion plug, the ion-selective electrode that water conservancy diversion is equipped with beyond the Great Wall, which passes through, adjusts lock and cock and sample Product reservoir.This device can carry out photoelectric synchronous analysis to sample, and detection efficiency is high, and high sensitivity is easy to operate, at low cost.
Description
Technical field
The utility model relates to cell and mitochondria activity Indexs measure analysis field, especially a kind of cell and mitochondria
A variety of physiological activity index Synchronization Analysis devices.
Background technique
Spectrophotometer is that the light of complicated component is decomposed into the scientific instrument of spectrum line, can be carried out to substance qualitative
Or quantitative analysis, it can be used for the measurement of bioactivity related substances concentration；Clark oxygen electrode is to measure content of oxygen dissolved in water
And a kind of polarographic electrode designed, it can be used for measuring cell or mitochondria/chloroplaset respiration or photosynthesis；Ion choosing
Specific ion can be identified by selecting electrode, by potential calculation ion concentration, can measure cell or organelle and certain ion phase
The physiological activity index and membrane potential of pass.Three of the above analytical equipment is relatively conventional in laboratory.Biology or medical research
The analysis that a variety of physiological activity indexs are carried out to same sample is generally required in experimentation, but existing analysis instrument is mostly
Individual equipment, every kind of instrument can only analyze a kind of physiological activity index, obtain a variety of physiological activity indexs, need to consume big
The sample of amount and time can greatly save experimental cost and time, and energy if analysis can be synchronized to same sample
More accurately obtain the relationship between each physical signs.And the instrument for possessing these three detection functions simultaneously at present yet there are no report
Road, the instrument price for having two of them synchronism detection function are also costly.
Utility model content
The utility model aim be for existing delay generator it is expensive, the relatively low deficiency of cost performance provides
A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria.This device can carry out photoelectric synchronous point to sample
Analysis, and detection efficiency is high, response is fast, and high sensitivity is easy to operate, at low cost.
Realizing the technical solution of the utility model aim is:
A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria, including the inspection of sample detection unit, reference
Surveying unit, ultraviolet specrophotometer, the first data acquisition circuit and the second data acquisition circuit, sample detection unit includes constant temperature
Set, positioning housing, sample container, oxygen electrode and blender, the constant temperature sleeve is hollow cube, and outer wall is equipped with baffle,
Pipeline is equipped in constant temperature sleeve, the positioning housing is equipped with protrusion, and protrusion is the cavity of upper end opening, the lug boss of positioning housing
Go deep into the hollow position of constant temperature sleeve from constant temperature sleeve bottom, the bottom of positioning housing is successively equipped with blender, oxygen electrode from bottom to top
And sample container, the upper end of positioning housing protrusion are successively fitted with cock, adjusting lock and water conservancy diversion plug, water conservancy diversion from bottom to top and set beyond the Great Wall
Some ion-selective electrodes pass through adjusting lock and cock is connected to sample container, reference detection unit and sample detection cellular construction
Equally, the first data acquisition circuit connects ion-selective electrode, and the second data acquisition circuit connects oxygen electrode, the acquisition of the first data
Circuit, the second data acquisition circuit and ultraviolet specrophotometer are circumscribed with processing terminal, and sample detection unit and reference detection are single
Member is placed in ultraviolet specrophotometer.
Sample container is the cavity of upper end opening, and the outer wall of cavity lower part is equipped with symmetrical first optical transmission window and second thoroughly
It is set respectively at the position of the corresponding covering positioning housing of light window, the first optical transmission window and the second optical transmission window, constant temperature sleeve and baffle
There are first through hole and the second through-hole, and first through hole and the second via area are greater than corresponding first optical transmission window and the second light transmission
Window area, the beam path of the first optical transmission window and the second optical transmission window face ultraviolet specrophotometer are used for spectral detection.
Constant temperature sleeve is equipped with inlet and outlet and is connected to pipeline.
Reference detection unit and sample detection unit periphery are equipped with carrier, can cooperate light adjust reference detection unit and
The height of sample detection unit.
First data acquisition circuit acquires data using ionometer.
The circuit acquisition data that second data acquisition circuit mainly uses STM32F030K6T6 chip to construct.
Blender is screwed with positioning housing bottom, and fixed oxygen electrode.
Cock is screwed with positioning housing upper end, plays the role of adjusting water conservancy diversion plug position.
Constant temperature sleeve and baffle are made by aluminium alloy, conducive to the conduction of heat.
Sample container combines production by titanium alloy and quartz glass plate, and optical transmission window is quartz glass plate.
Water conservancy diversion plug is made by PEEK material, and lower end is tapered, it is therefore an objective to prevent sample from overflowing.
It adjusts lock to be made by POM plastic, play the role of locking water conservancy diversion plug and adjusts water conservancy diversion plug position.
The advantages of the utility model: can carry out photoelectric synchronous analysis to sample, and detection efficiency is high, and response is fast, high sensitivity,
It is easy to operate, it is at low cost.
Detailed description of the invention
Fig. 1 is embodiment connection schematic diagram；
Fig. 2 is the top view of sample detection unit and reference detection unit；
Fig. 3 is cross-sectional view at A-A in Fig. 2.
Specific embodiment
It is further elaborated below with reference to content of the embodiment and attached drawing to the utility model, but is not practical new to this
The restriction of type.
Embodiment:
Referring to Fig.1-3, a kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria, including sample detection list
Member 19, reference detection unit 20, ultraviolet specrophotometer 14, the first data acquisition circuit 16 and the second data acquisition circuit 17,
Sample detection unit 19 includes constant temperature sleeve 7, positioning housing 11, sample container 9, oxygen electrode 12 and blender 13, the constant temperature sleeve 7
For hollow cube, and outer wall is equipped with baffle 4, pipeline 8 is equipped in constant temperature sleeve 7, the positioning housing 11 is equipped with protrusion, convex
The cavity for upper end opening is played, the lug boss of positioning housing 11 gos deep into the hollow position of constant temperature sleeve 7 from 7 bottom of constant temperature sleeve, positions
The bottom of shell 11 is successively equipped with blender 13, oxygen electrode 12 and sample container 9, the upper end of 11 protrusion of positioning housing from bottom to top
It is successively fitted with cock 6 from bottom to top, adjusts lock 5 and water conservancy diversion plug 3, the ion-selective electrode 15 that water conservancy diversion plug 3 is equipped with, which passes through, to be adjusted
Section lock 5 and cock 6 are connected to sample container 9, and reference detection unit 20 as 19 structure of sample detection unit, adopt by the first data
The connection ion-selective electrode 15 of collector 16, the second data acquisition circuit 17 connection oxygen electrode 12, the first data acquisition circuit 16,
Second data acquisition circuit 17 and ultraviolet specrophotometer 14 are circumscribed with processing terminal 18, sample detection unit 19 and reference detection
Unit 20 is placed in ultraviolet specrophotometer 14.
Sample container 9 is the cavity of upper end opening, and the outer wall of cavity lower part is equipped with symmetrical first optical transmission window 10 and the
The corresponding covering positioning housing 11 of two optical transmission window 10-1, the first optical transmission window 10 and the second optical transmission window 10-1, constant temperature sleeve 7 and gear
First through hole 10-2 and the second through-hole 10-3, and first through hole 10-2 and the second face through-hole 10-3 are respectively equipped at the position of plate 4
Product is greater than corresponding first optical transmission window 10 and the second optical transmission window 10-1 area, the first optical transmission window 10 and the second optical transmission window
The beam path of 10-1 face ultraviolet specrophotometer 14 is used for spectral detection.
Constant temperature sleeve 7 is equipped with water inlet 2-1 and water outlet 2 is connected to pipeline 8.
Reference detection unit 20 and 19 periphery of sample detection unit are equipped with carrier 1, and light can be cooperated to adjust reference detection
The height of unit 20 and sample detection unit 19.
First data acquisition circuit 16 acquires data using ionometer.
Second data acquisition circuit 17 mainly acquires data using the circuit of STM32F030K6T6 chip building.
Blender 13 is screwed with 11 bottom of positioning housing, and fixed oxygen electrode 12.
Cock 6 is screwed with 11 upper end of positioning housing, plays the role of adjusting 3 position of water conservancy diversion plug.
Constant temperature sleeve 7 and baffle 4 are made by aluminium alloy, conducive to the conduction of heat.
Sample container 9 combines production by titanium alloy and quartz glass plate, and optical transmission window is quartz glass plate.
Water conservancy diversion plug 3 is made by PEEK material, and lower end is tapered, it is therefore an objective to prevent sample from overflowing.
It adjusts lock 5 to be made by POM plastic, play the role of locking water conservancy diversion plug 3 and adjusts 3 position of water conservancy diversion plug.
Carrier 1, sample detection unit 19 and reference detection unit 20 are placed in ultraviolet specrophotometer 14, by leading
Stream plug 3 injects a sample into the sample container 9 of sample detection unit 19, opens water inlet 2-1, the water outlet of sample detection unit 19
2 and the water of pipeline 8 so that the temperature of sample detection unit is reached target temperature, followed by what is be equipped in ultraviolet specrophotometer 14
The optical data of light source and analysis system acquisition sample, while the optical data of no sample in reference detection unit 20 is acquired, it uses
The optical data of sample deducts the optical data of no sample, can be used to the measurement of bioactivity related substances concentration；Carry out
While optical analysis, oxygen electrode 12 is synchronous with ion-selective electrode 15 to detect sample, acquires electricity by the first data
The analysis of road 16 and the second data acquisition circuit 17 is converted, and the data transmission of acquisition is directly displayed current potential into processing terminal 18
Variation can carry out potentiometric analysis to the relevant physiologicals active procedure such as respiration, photosynthesis or membrane potential.
Claims (4)
1. a kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria, including the detection of sample detection unit, reference
Unit, ultraviolet specrophotometer, the first data acquisition circuit and the second data acquisition circuit, which is characterized in that sample detection list
Member includes constant temperature sleeve, positioning housing, sample container, oxygen electrode and blender, and the constant temperature sleeve is hollow cube, and outer wall
Equipped with baffle, pipeline is equipped in constant temperature sleeve, the positioning housing is equipped with protrusion, and protrusion is the cavity of upper end opening, positions shell
The lug boss of body gos deep into the hollow position of constant temperature sleeve from constant temperature sleeve bottom, and the bottom of positioning housing is successively equipped with stirring from bottom to top
Device, oxygen electrode and sample container, the upper end of positioning housing protrusion are successively fitted with cock from bottom to top, adjust lock and water conservancy diversion plug,
The ion-selective electrode that water conservancy diversion is equipped with beyond the Great Wall passes through adjusting lock and cock is connected to sample container, and reference detection unit and sample are examined
It is the same to survey cellular construction, the first data acquisition circuit connects ion-selective electrode, and the second data acquisition circuit connects oxygen electrode, the
One data acquisition circuit, the second data acquisition circuit and ultraviolet specrophotometer are circumscribed with processing terminal, sample detection unit and
Reference detection unit is placed in ultraviolet specrophotometer.
2. analytical equipment as described in claim 1, which is characterized in that sample container is the cavity of upper end opening, cavity lower part
Outer wall be equipped with symmetrical first optical transmission window and the second optical transmission window, the corresponding covering of the first optical transmission window and the second optical transmission window
First through hole and the second through-hole, and first through hole and the second through-hole are respectively equipped at the position of positioning housing, constant temperature sleeve and baffle
Area is greater than corresponding first optical transmission window and the second optical transmission window area.
3. analytical equipment as described in claim 1, which is characterized in that constant temperature sleeve is equipped with inlet and outlet and pipeline connects
It is logical.
4. analytical equipment as described in claim 1, which is characterized in that reference detection unit and sample detection unit periphery are equipped with
Carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820529162.8U CN208187975U (en) | 2018-04-15 | 2018-04-15 | A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820529162.8U CN208187975U (en) | 2018-04-15 | 2018-04-15 | A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208187975U true CN208187975U (en) | 2018-12-04 |
Family
ID=64433414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820529162.8U Expired - Fee Related CN208187975U (en) | 2018-04-15 | 2018-04-15 | A kind of cell and a variety of physiological activity index Synchronization Analysis devices of mitochondria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208187975U (en) |
-
2018
- 2018-04-15 CN CN201820529162.8U patent/CN208187975U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181204 |