CN208130077U - A kind of hollow tubular structure based on hydrogel - Google Patents
A kind of hollow tubular structure based on hydrogel Download PDFInfo
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- CN208130077U CN208130077U CN201720754545.0U CN201720754545U CN208130077U CN 208130077 U CN208130077 U CN 208130077U CN 201720754545 U CN201720754545 U CN 201720754545U CN 208130077 U CN208130077 U CN 208130077U
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Abstract
The utility model discloses a kind of hollow tubular structures based on hydrogel.The hollow tubular structure includes a hollow lumen and the several layers gel layer that is coated on the outer wall of the hollow lumen；The cross section of the hollow lumen is round, square, trapezoidal, pentalpha, polygon or other special-shaped closed figures；The hollow lumen has single or more bifurcation structures.The hollow tubular structure of the utility model, the hydrogel structure with multilayer, the geometric shape of hollow lumen therein is flexible, can bifurcated, variable-diameter, shape-variable can more meet the bionical demand of the live tissues such as blood vessel, tracheae；And the hydrogel structure can load cell, have broad prospect of application.
Description
Technical field
The utility model relates to a kind of hollow tubular structures based on hydrogel.
Background technique
Hollow tubular structure is characteristics of organizational structure, such as blood vessel, tracheae, bile duct, enteron aisle etc. common in a kind of human body.
Meanwhile often there is the feature of multilayer wall surface in these tubular structures, in vitro in Bionic Design and manufacture, to this multilayer hollow
The building of tubular structure is a great demand, especially in terms of tissue engineering vessel.Current organizational project skill
Art mostly uses the microfluidic device of Coaxial nozzle or coaxial runner when constructing bionical blood vessel, squeeze out it is of uniform size, without bifurcated
Single hollow tube, (with the increase of tube wall layer number, device complexity is got higher, behaviour there are certain limitation for the number of plies of tube wall
Make it is unstable, at present these methods form 3 layers or less it is in the majority).
Utility model content
The purpose of the utility model is to provide a kind of hollow tubular structures, specifically based on the hollow tubular structure of hydrogel,
Various structures.
Hollow tubular structure provided by the utility model, it includes a hollow lumen and is coated on the outer of the hollow lumen
Several layers gel layer on wall.
In the hollow tubular structure, the cross section of the hollow lumen can for round, square, trapezoidal, pentalpha,
Polygon or other special-shaped closed figures.
In the hollow tubular structure, the hollow lumen has single or more bifurcation structures.
In the hollow tubular structure, each axis of more bifurcation structures is in two-dimensional surface or three-dimensional space.
In the hollow tubular structure, in the axial direction, the shape and/or size of the cross section of the hollow lumen occur
Variation such as fades to square column from thick to thin, by cylinder.
In the hollow tubular structure, the hollow lumen has second level bifurcation structure.
In the hollow tubular structure, the internal diameter of the hollow lumen is 0.05~50mm, can be determined according to concrete application
Its internal diameter.
In the hollow tubular structure, the hollow tubular structure includes 1~15 layer of gel layer, can be according to concrete application
Determine the number of plies.
In the hollow tubular structure, the thickness of the gel layer is equal or different.
In the utility model hollow tubular structure, alginate layer is can be used in the gel layer, specifically can be by alginic acid
Sodium and the crosslinked formation of bivalent cation such as form calcium alginate gel layer using sodium alginate and calcium ion crosslinking.The sea
The thickness of alginate jelly layer (can be crosslinked) time by contact of the control sodium alginate with bivalent cation and is adjusted.
In order to extend the application of the hollow tubular structure, other components can be added in the gel layer, such as 1) collagen, fibre
It is any in fibrillarin original, matrigel, fibronectin, hyaluronic acid, gelatin, chitosan and polyethylene glycol, i.e., it is natural or synthetic
The ingredients such as albumen, polysaccharide：2) endothelial cell, smooth muscle cell, fibroblast, cartilage cell, liver cell, cardiac muscle cell,
The various adult stem cells of nerve cell cancer cell, embryonic stem cell and in inducing multi-potent stem cell it is any, i.e., various lactations are dynamic
Object primary cell or mature cell system；3) bacterium or virus；4) drug and/or nucleic acid molecules, such as anticancer drug, siRNA etc.；
Said components can be loaded into the gel layer by making an addition to the mode in sodium alginate soln.
In the utility model hollow tubular structure, the hollow lumen can be obtained by the molding of thermo-sensitive gel core with dissolution,
The shape of the thermo-sensitive gel core determines the shape of the hollow lumen.The thermo-sensitive gel core can obtain in the following way
It arrives：
1) temperature sensitive solution is poured into using in mold made of the materials such as metal, high molecule plastic, macromolecular elastomer
(for example can customize mold using 3D printing technique printing), waits gel completion to take out stand-by.
2) it draws temperature sensitive solution with syringe, gel is waited to complete, front end of the syringe needle is truncated, gel core is pushed out to obtain the final product
To with the consistent gel core of syringe inside dimension.
3) 3D printing method is used, temperature sensitive solution is printed as desired shape and carries out gel.
The thermo-sensitive gel used can be gelatin, modified gelatin, pluronic F127, chitosan-based hydrogel, agarose, poly-
Any in acrylamide and matrigel, they have temperature sensitive reversible crosslink characteristic；
The modified gelatin refers to the gelatin obtained by various chemical modifications, still keeps temperature-sensing property, such as gelatin
Methacrylamide.
Different types of thermo-sensitive gel, forming temperature is also different, and e.g., for gelatin solution, gel molding temperature is in
Lower temperature is can be controlled within the scope of 0~25 DEG C according to its concentration；For the material of high temperature gel cryosol, gel forming
Temperature is in higher temperature, as the forming temperature of pluronic F127 can be at 20~50 DEG C.As in the embodiment of the present invention for
The gelatin of various concentration, it is applicable using 4 DEG C of forming temperature；For the pluronic F127 of various concentration, using 22.5
DEG C, 30 DEG C, 37 DEG C of forming temperature, it is applicable.
When the utility model hollow tubular structure uses more bifurcation structures, it can be applied to building engineering three-dimensional tissue structures, as by institute
It states hollow tubular structure to be placed in matched tissue forming cavity, exposes the inlet and outlet nozzle of perfusion culture；In the hollow tubular structure
Around, the structure of the cell containing specific organization is formed into tissue forming cavity；
The tissue forming cavity can be the open-topped cavity with specific shape, to the prefabricated blood vessel of fixation
Net, and form specific organization；Its material can be the elastomers such as the plastics such as ABS, PLA or PDMS, the manufacturer of the forming cavity
Method can be casting or direct 3D printing.
The engineering three-dimensional tissue structures have the following advantages that：1) using hollow tubular structure, (prefabricated bionical rete vasculosum, that is, be loaded with
Cell), improve the nutrition transport efficiency of tissue；2) tissue expansion is good, the characteristics of according to destination organization, can carry out difference
The manufacture of bionical blood vessel network；3) prefabricated bionical rete vasculosum and the direct printing technique of cell are combined, it can be on the basis of rete vasculosum
The tissue of upper molding foreign cell component, has stronger bionical effect.
Hollow tubular structure and engineering three-dimensional tissue structures of the utility model based on hydrogel have potential in bioengineering
Application, be such as used for：
1) reparation and reconstruction of tissue and organ can be directed to the organizational projects such as cardiac muscle, liver, kidney, bone, cartilage, into
The corresponding tissue construction of row；
2) research of pathological model is based on prefabricated rete vasculosum, can study the lesions such as cancer migration under corresponding physiology scene
Rule；
3) drug test is based on prefabricated rete vasculosum, can be with the release and effect of aids drug, and the tissue mould based on building
Type detects the effect of certain drug.
The hollow tubular structure of the utility model, the hydrogel structure with multilayer, the geometric shape of hollow lumen therein
Flexibly, can bifurcated, variable-diameter, shape-variable can more meet the bionical demand of the live tissues such as blood vessel, tracheae；And institute
Cell can be loaded by stating hydrogel structure, have broad prospect of application.
Detailed description of the invention
Fig. 1 is the schematic diagram for the hollow tubular structure that the utility model has two order bifurcation.
It is respectively marked in figure as follows：
1 hollow lumen, 2 gel layers.
Specific embodiment
The utility model is described further with reference to the accompanying drawing, but the utility model is not limited to following implementation
Example.
As shown in Figure 1, having the schematic diagram of the hollow tubular structure of two order bifurcation for the utility model, left figure is whole signal
Figure, right figure are longitudinal section and the cross-sectional view of a bifurcation structure, it includes hollow lumen 1 and is coated on hollow lumen 1
2,2 partial enlarged views of gel layer on outer wall from top to bottom respectively indicate Longitudinal cross section schematic and the cross section of hollow lumen 1
Schematic diagram, it can be seen that the gel layer 2 for the equal thickness that is of five storeys is coated on the outer wall of hollow lumen 1.Hollow lumen 1 has two fractions
Structure is pitched, cross section is circle, has certain diameter change by limb to bifurcateds at different levels, limb internal diameter is 3mm, a fraction
Fork internal diameter is 2.5mm, and second level bifurcated internal diameter is 2mm.；Each axis of hollow lumen 1 is in two-dimensional surface.
In the present embodiment, gel layer 2 specifically is formed to obtain by calcium alginate gel, is specifically made by the following method
It is standby：The gel core with second level bifurcation structure is prepared as raw material using the gelatin for being mixed with bivalent cation first, limb internal diameter is
3mm, primary furcation internal diameter are 2.5mm, and second level bifurcated internal diameter is 2mm；Then gel core is soaked in sodium alginate soln, is passed through
Cross-linking reaction coats layer of gel in gel wicking surface, after taking-up, repeats above-mentioned soaking step 4 times, i.e., in gel core
Surface coats 4 layers of gel layer, and the hollow tubular structure with two order bifurcation is arrived in dissolution after the dissolution of gel core.
It can be used for constructing engineering three-dimensional tissue structures using the utility model hollow tubular structure, hollow tubular structure be such as placed in matching
Tissue forming cavity in, expose perfusion culture inlet and outlet nozzle；Around hollow tubular structure, forms and contain into tissue forming cavity
The structure of specific organization's cell constructs the three-dimensional thick tissue structure around the hollow tubular structure, previously fabricated hollow tube
Structure can provide nutriment and oxygen necessary to cell for thick tissue.
In above-mentioned building process, tissue forming cavity can be the open-topped cavity with specific shape, to
Fixed prefabricated rete vasculosum, and form specific organization；Its material can be the elastomers such as the plastics such as ABS, PLA or PDMS, this at
The manufacturing method of type chamber can be casting or direct 3D printing.
In above-mentioned building process, organize molding mode that can be：1) water-setting containing cell is directly poured into tissue forming cavity
Glue or pure cell solution；2) that adds can carry out crosslinking Treatment or without crosslinking Treatment containing cell component；3) to being organized into
Specific histocyte is directly printed in type chamber, by different types of hydrogel or cell printing at not around prefabricated rete vasculosum
Same structure, to promote the generation of tissue；4) printing of institutional framework and the printing of forming cavity can carry out simultaneously；5) it is organizing
In forming process, casting and 3D printing method can be carried out concurrently or separately.
By the building of the three-dimensional thick tissue containing rete vasculosum (the utility model hollow tubular structure), can use prefabricated bionical
Rete vasculosum improves the nutrition transport efficiency of tissue；It organizes expansion good, the characteristics of according to destination organization, can carry out different imitative
The manufacture of green blood managed network；In conjunction with prefabricated bionical rete vasculosum and the direct printing technique of cell, can on the basis of rete vasculosum at
The tissue of type foreign cell component has stronger bionical effect.
Claims (1)
1. a kind of hollow tubular structure, it is characterised in that：The hollow tubular structure includes a hollow lumen and is coated on described hollow
1 ~ 15 layer of gel layer on the outer wall of lumen；
The cross section of the hollow lumen is round, square, trapezoidal, pentalpha, polygon or other special-shaped closed figures；
The hollow lumen has single or more bifurcation structures；
Each axis of more bifurcation structures is in two-dimensional surface or three-dimensional space；
In the axial direction, the shape and/or size of the cross section of the hollow lumen change；
The hollow lumen has second level bifurcation structure；
The internal diameter of the hollow lumen is 0.05 ~ 50mm；
The thickness of the gel layer is equal or different.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720754545.0U CN208130077U (en) | 2017-06-27 | 2017-06-27 | A kind of hollow tubular structure based on hydrogel |
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| CN201720754545.0U CN208130077U (en) | 2017-06-27 | 2017-06-27 | A kind of hollow tubular structure based on hydrogel |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662803A (en) * | 2019-01-18 | 2019-04-23 | 东南大学苏州医疗器械研究院 | A kind of artificial blood vessel generates mold and culture system |
| CN113017944A (en) * | 2019-12-25 | 2021-06-25 | 广东省人民医院(广东省医学科学院) | Artificial blood vessel stent with bioactivity, preparation method and application thereof |
| CN114470328A (en) * | 2022-01-25 | 2022-05-13 | 哈尔滨工业大学 | Manufacturing method of 3D printing biological ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel bracket |
-
2017
- 2017-06-27 CN CN201720754545.0U patent/CN208130077U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662803A (en) * | 2019-01-18 | 2019-04-23 | 东南大学苏州医疗器械研究院 | A kind of artificial blood vessel generates mold and culture system |
| CN113017944A (en) * | 2019-12-25 | 2021-06-25 | 广东省人民医院(广东省医学科学院) | Artificial blood vessel stent with bioactivity, preparation method and application thereof |
| CN114470328A (en) * | 2022-01-25 | 2022-05-13 | 哈尔滨工业大学 | Manufacturing method of 3D printing biological ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel bracket |
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