CN208060352U - A kind of device suitable for micro- plastic sample quantitative analysis and counting - Google Patents
A kind of device suitable for micro- plastic sample quantitative analysis and counting Download PDFInfo
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- CN208060352U CN208060352U CN201820678969.8U CN201820678969U CN208060352U CN 208060352 U CN208060352 U CN 208060352U CN 201820678969 U CN201820678969 U CN 201820678969U CN 208060352 U CN208060352 U CN 208060352U
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- groove
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- quantitative analysis
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Abstract
The utility model discloses a kind of device suitable for micro- plastic sample quantitative analysis and counting, including being suitable for the microscopical opaque tempered glass support plate of body, the identical ultra-thin hydrophobic high transparent glass cover board with the support plate phase shape, the support plate and cover board are closely connected as to the geometrical clamp of an entirety, the support plate gabarit is rectangle, in one side center, one circular groove is set, the bottom portion of groove frosted processing, the groove area is slightly larger than the area of standard microwell filter membrane, and the depth of groove is slightly larger than the thickness of standard microwell filter membrane；The cover board side goes out sample observation area corresponding to the position of the groove by laser ablation, the sample observation area includes an annulus identical with the groove profile, the rice wordline that intersection point is overlapped with the annulus center of circle, the annulus and rice wordline form the fan-shaped count block of 8 area equations.The utility model has stability good, and counting precision is high, and adaptability is good.
Description
Technical field
It is specifically a kind of to be suitable for micro- plastic sample quantitative analysis and meter the utility model is related to Microbeam Analysis Techniques field
Several devices.
Background technology
Plastic products because the characteristics such as its Portable durable, of low cost are widely used in industrial and agricultural production and daily life,
A large amount of plastic products eventually enter into and are enriched with Yu Haiyang.These plastic products are not degradable in the natural environment, but in illumination, tide
Micro- plastic grain that size is less than 5 mm can be broken under the effects that nighttide, wave, the temperature difference or wind are lost.Micro- plastic grain physics is special
Property stablize, degradation speed is extremely slow, can be persistently enriched in water environment, be a variety of poisonous and harmful substances transfer among carry
Body, and human body can be entered by channels such as food, drinking water, human health is threatened, it also can be each to being generated to other biological
Kind harm.In recent years, the micro- plastic pollution problem in ocean causes the highest attention of coastal state, while also causing external many
Person payes attention to this emerging pollutant.
Quantitative analysis is carried out to micro- plastic sample in laboratory at present and mainly uses stereomicroscope microscopy with quantity survey (surveying)
Method.Operating method is to pass through micro- plastic sample solution after pre-treatment to be placed with diameter 47mm, and the standard that 0.45 μm of aperture is micro-
The vacuum pump Suction filtration device of hole filter membrane filters, and filtered miillpore filter is then placed in observation under stereomicroscope and is counted.
It is larger or be unevenly distributed situation to find that micro- plastic sample on miillpore filter is frequently present of quantity in experiment, is difficult to micro- at this time
Plastics storage carries out more accurate quantitative analysis and quantity survey (surveying).If directly carried out to miillpore filter using stereomicroscope
Observation counts, and because miillpore filter is smooth wafer architecture, no identification point there are problems that counting difficult or repeat count.If
Micro- plastic sample on miillpore filter is chosen into counting one by one, can not only be taken a substantial amount of time and energy, but also because of micro- plastics
Volume is small, and there are larger omission factors., the media such as air present in micro- plastic optical fibre longer because selecting time consumption simultaneously
Contaminated samples probability is larger, and count results are inaccurate.Existing blood counting chamber or planktonic organism tally are such as borrowed to micropore
Micro- plastic sample quantitative analysis on filter membrane, since it is designed not directed to the characteristics of micro- plastics, use environment there is also
Larger difference, there are the inconvenient for use and larger problems of error, and can be direct to micro- plastic sample when the above-mentioned counting device of use
Contact compressing causes the micro- plastic sample deformation or broken in part, it is difficult to identification or accurate judgement.Lack now a kind of suitable for micro-
The counting device of plastic sample quantitative analysis and quantity survey (surveying).
Utility model content
For a series of defects for carrying out quantitative analysis and quantity survey (surveying) method in existing laboratory to micro- plastic sample, originally
Utility model discloses a kind of convenient and efficient and more accurately can carry out quantitative analysis and quantity survey (surveying) to micro- plastic sample
Counting device.
To achieve the goals above, the technical solution that the utility model is taken is as follows：
A kind of device suitable for micro- plastic sample quantitative analysis and counting includes suitable for the opaque of stereomicroscope
Tempered glass support plate, the identical ultra-thin hydrophobic high transparent glass cover board with the support plate phase shape, the support plate and cover board are closely connected
It is connected in the geometrical clamp of an entirety, it is characterised in that：The support plate gabarit is rectangle, and it is recessed that a circle is arranged in one side center
Slot, the bottom portion of groove frosted processing, the groove area are slightly larger than the area of standard microwell filter membrane, and the depth of groove is bigger
In the thickness of standard microwell filter membrane；The cover board side goes out sample observation corresponding to the position of the groove by laser ablation
Area, the sample observation area include an annulus identical with the groove profile, the rice that intersection point is overlapped with the annulus center of circle
Wordline, the annulus and rice wordline form the fan-shaped count block of 8 area equations.
It is also possible that the cover board side, corresponding to the position of the groove going out sample observation area, institute by laser ablation
It includes an annulus identical with the groove profile to state sample observation area, and four form the box that square is inscribed in the annulus
Line, four well wordline that the inscribed square is divided into nine equal portions, the annulus, box line and well wordline form 9 nine
Palace count block.
Further, the annulus and the line thickness of rice wordline are 40 μm.
Further, the box line and the line thickness of well wordline are 40 μm.
Further, if each nine palace count block is mutually perpendicular to by several and the identical latitude and longitude of spacing are divided into
It is dry to count grid.
Further, the line thickness of the latitude and longitude is 20 μm.
The present apparatus has stability good, and counting precision is high, and the good feature of adaptability can be widely applied to strand tidal flat, water
Micro- plastic sample quantitative analysis such as body, soil, bottom sediment and quantity survey (surveying) work.With the prior art compared with method, this
Utility model advantage is as follows：It is larger or be unevenly distributed situation that 1. micro- plastic sample on miillpore filter is frequently present of quantity, this
When be difficult accurately to be estimated micro- plastics storage, the utility model counting device can be conveniently to micro- on miillpore filter
Plastic sample carries out quantitative analysis and counts；2. directly observation counting is carried out to miillpore filter using stereomicroscope, because of micropore
Filter membrane is smooth wafer architecture, and no identification point there are problems that counting difficult or repeat count.The utility model counting device has
Standard counts grid or rice word count area, can quickly position, accurately estimate micro- plastics storage；3. the groove of this counting device support plate
A closed space can be formed with cover plate combination is counted, miillpore filter, which is placed in one, to be contacted with the external world, avoid because of water
Divide evaporation to cause the Texturized situation of filter membrane, and avoids the existing micro- plastic optical fibre contaminated samples of floating in the media such as air and make
At the inaccurate situation of counting；4. micro- plastic sample on miillpore filter is such as chosen counting one by one, not only take time and effort, Er Qieyin
Micro- plastics volume is small, and there are larger omission factors.It is longer and be directly exposed in air, Jie such as air because selecting the time simultaneously
Micro- plastic optical fibre contaminated samples probability present in matter is larger.Miillpore filter can directly be set by the utility model counting device
It is observed under stereomicroscope, time saving and energy saving, comfort is good.5. the utility model counting device for micro- plastic grain feature into
Row design, sample observation area will not oppress sample when counting there are fluted.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram of the utility model support plate.
Fig. 3 is the structural schematic diagram of the cover board of the utility model embodiment 1.
Fig. 4 is the structural schematic diagram of the cover board of the utility model embodiment 2.
Fig. 5 is the partial enlarged view of Fig. 4.
Specific implementation mode
The utility model counting device is further described with the following Examples.
It should be noted that the following content is make further specifically to the utility model in conjunction with specific embodiments
It is bright, but it cannot be assumed that the present embodiment is only limitted to this, under the guidance of embodiment, research those skilled in the art can be in this implementation
Example on the basis of carry out various improvement or optimization, these improve or optimization fall it is within the protection scope of the present utility model.
Embodiment 1, referring to attached drawing 1-3, a kind of device suitable for micro- plastic sample quantitative analysis and counting, including be applicable in
Opaque tempered glass support plate 1 in stereomicroscope, the identical ultra-thin hydrophobic high transparent glass cover board 2 with 1 phase shape of the support plate,
By the support plate 1 and the close geometrical clamp 3 for being connected as an entirety of cover board 2,1 gabarit of the support plate is rectangle, in one side
One circular groove 11 of centre setting, the bottom portion of groove frosted processing, the groove area are slightly larger than the face of standard microwell filter membrane
Product, the depth of groove are slightly larger than the thickness of standard microwell filter membrane, are advisable with placing not prominent surface after standard microwell filter membrane；?
Ultra-pure water is used to soak frosted portion before placing the standard microwell filter membrane with micro- plastic sample, to ensure that standard microwell filter membrane pastes
Conjunction does not tilt；2 side of the cover board goes out sample observation area 21 corresponding to the position of the groove 11 by laser ablation, described
Sample observation area 21 includes an annulus 211 identical with 11 profile of the groove, the rice that intersection point is overlapped with the annulus center of circle
Wordline 212, the annulus 211 and rice wordline 212 form the fan-shaped count block 22 of 8 area equations；The annulus 211 and rice word
The line thickness of line 212 is 40 μm.The cover board of embodiment 1 is suitable for the situation of micro- plastic sample negligible amounts on miillpore filter.
Embodiment 2, referring to attached drawing 1,2,4,5, a kind of device suitable for micro- plastic sample quantitative analysis and counting, including
Opaque tempered glass support plate 1 suitable for stereomicroscope, the identical ultra-thin hydrophobic high transparent glass lid with 1 phase shape of the support plate
Plate 2, by the support plate 1 and the close geometrical clamp 3 for being connected as an entirety of cover board 2, it is characterised in that：1 gabarit of the support plate is
A circular groove 11 is arranged in one side center in rectangle, and the bottom portion of groove frosted processing, the groove area is slightly larger than mark
The area of quasi- miillpore filter, the depth of groove are slightly larger than the thickness of standard microwell filter membrane；2 side of the cover board corresponds to institute
The position for stating groove 11 goes out sample observation area 21 by laser ablation, and the sample observation area 21 includes one and the groove 11
The identical annulus 211 of profile, four form the box lines 213 that square is inscribed in the annulus, and four by the inscribed square
It is divided into the well wordline 214 of nine equal portions, the box line 213 and well wordline 214 to form 9 nine palace count blocks 24；It is each described
Nine palaces count block 24 are mutually perpendicular to by 20 and the identical latitude and longitude of spacing 215 are divided into 100 counting grids 25, the box
Line 213 and the line thickness of well wordline 214 are 40 μm.The line thickness of the latitude and longitude 215 is 20 μm.The cover board of embodiment 2
Suitable for micro- numerous situations of plastic sample quantity on miillpore filter.
Claims (6)
1. a kind of device suitable for micro- plastic sample quantitative analysis and counting includes the opaque steel suitable for stereomicroscope
Change glass support plate（1）And the support plate（1）The identical ultra-thin hydrophobic high transparent glass cover board of phase shape（2）, by the support plate（1）With
Cover board（2）The close geometrical clamp for being connected as an entirety（3）, it is characterised in that：The support plate（1）Gabarit is rectangle, in one
A circular groove is arranged in side center（11）, the bottom portion of groove frosted processing, the groove area is slightly larger than standard microwell filter
The area of film, the depth of groove are slightly larger than the thickness of standard microwell filter membrane；The cover board（2）Side corresponds to the groove
（11）Position sample observation area is gone out by laser ablation（21）, the sample observation area（21）Including one and the groove
（11）The identical annulus of profile（211）, rice wordline that intersection point is overlapped with the annulus center of circle（212）, the annulus（211）And rice
Wordline（212）Form the fan-shaped count block of 8 area equations（22）.
2. a kind of device suitable for micro- plastic sample quantitative analysis and counting according to claim 1, it is characterised in that：
The cover board（2）Side corresponds to the groove（11）Position sample observation area is gone out by laser ablation（21）, the sample
Observation area（21）Including one and the groove（11）The identical annulus of profile（211）, four form the inscribed pros of the annulus
The box line of shape（213）, four well wordline that the inscribed square is divided into nine equal portions（214）, the box line（213）
With well wordline（214）Form 9 nine palace count blocks（24）.
3. a kind of device suitable for micro- plastic sample quantitative analysis and counting according to claim 1, it is characterised in that：
The annulus（211）With rice wordline（212）Line thickness be 40 μm.
4. a kind of device suitable for micro- plastic sample quantitative analysis and counting according to claim 2, it is characterised in that：
The box line（213）With well wordline（214）Line thickness be 40 μm.
5. a kind of device suitable for micro- plastic sample quantitative analysis and counting according to claim 2, it is characterised in that：
Each nine palace count block（24）It is mutually perpendicular to and the identical latitude and longitude of spacing by several（215）It is divided into several counting sides
Lattice（25）.
6. a kind of device suitable for micro- plastic sample quantitative analysis and counting according to claim 5, it is characterised in that：
The latitude and longitude（215）Line thickness be 20 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820678969.8U CN208060352U (en) | 2018-05-08 | 2018-05-08 | A kind of device suitable for micro- plastic sample quantitative analysis and counting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820678969.8U CN208060352U (en) | 2018-05-08 | 2018-05-08 | A kind of device suitable for micro- plastic sample quantitative analysis and counting |
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| Publication Number | Publication Date |
|---|---|
| CN208060352U true CN208060352U (en) | 2018-11-06 |
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| CN201820678969.8U Expired - Fee Related CN208060352U (en) | 2018-05-08 | 2018-05-08 | A kind of device suitable for micro- plastic sample quantitative analysis and counting |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286004A (en) * | 2019-07-11 | 2019-09-27 | 叶建锋 | The acquisition method and device of a kind of micro- plastics sedimentation of atmosphere for different height |
-
2018
- 2018-05-08 CN CN201820678969.8U patent/CN208060352U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286004A (en) * | 2019-07-11 | 2019-09-27 | 叶建锋 | The acquisition method and device of a kind of micro- plastics sedimentation of atmosphere for different height |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181106 Termination date: 20190508 |