CN208049951U - A kind of micro-fluidic chip and micro-fluidic detection device - Google Patents
A kind of micro-fluidic chip and micro-fluidic detection device Download PDFInfo
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- CN208049951U CN208049951U CN201820194580.6U CN201820194580U CN208049951U CN 208049951 U CN208049951 U CN 208049951U CN 201820194580 U CN201820194580 U CN 201820194580U CN 208049951 U CN208049951 U CN 208049951U
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Abstract
The utility model belongs to technical field of medical detection more particularly to a kind of micro-fluidic chip and micro-fluidic detection device.The utility model provides a kind of micro-fluidic chip, including micro-fluidic chip substrate, determinand sensing chamber and side throwing polishing fibre；Side throwing polishing fibre is fixed at the surface of micro-fluidic chip substrate, and the fine side throwing mill area's opening upwards of side throwing polishing are arranged on the surface of micro-fluidic chip substrate；Determinand sensing chamber is wrapped in the periphery in the fine side throwing mill area of side throwing polishing so that determinand is placed in the side throwing mill area of determinand sensing chamber.The invention also discloses a kind of micro-fluidic detection devices.The utility model solves the detection sensitivity of traditional micro-fluidic chip and accuracy is low and the excessive technological deficiency of micro-fluidic detection device.
Description
Technical field
The utility model belongs to technical field of medical detection more particularly to a kind of micro-fluidic chip and micro-fluidic detection dress
It sets.
Background technology
The content of material composition is analyzed in chemistry, biology and medical domain are frequently necessary to solution, is divided light
Degree meter is to measure the common instrument and equipment of material composition content in solution, is had in chemistry, environmental science, biology and medical domain
It and is widely applied.Spectrophotometer is mainly by light source, monochromator, sample room, detector, signal processor and display and storage
System forms.
Micro-fluidic chip has more and more in organic synthesis, microreactor, chemical analysis and biomedical sector at present
Application, microflow control technique refers to using microchannel (size be tens of arrive hundreds of microns) processing or manipulating minute fluid (volume
For nanoliter to microlitre) involved by Science and Technology, have micromation, integrated feature.Micro-fluidic chip is micro-fluidic skill
The main realization platform of art, microfluidic chip technology (Microfluidics) are the samples biology, chemistry, medical analysis process
The basic operation units such as this preparation, reaction, separation, detection are integrated on the chip of one piece of micro-meter scale, and it is complete to be automatically performed analysis
Process.Micro-fluidic chip have liquid flowing is controllable, reagent need to be consumed and sample size is few, analyze speed is fast, multifunctional unit,
The advantages that small and easy to carry.
In recent years, in micro-fluidic field, with the continuous improvement that microflow control technique requires, the microchannel of micro-fluidic chip
Size gradually reduces.However, for the micro-fluidic chip that microchannel size further reduces, using conventional laser direct irradiation
Mode, or the method that is embedded to optical fiber in the chip, can be due to fluorescence excitation device it is oversized, plane of illumination is big and interferes
The fluorescence signal of sample in microchannel, to influence the detection sensitivity and reliability of sample, in addition, existing spectrophotometric
The volume that the optical detection apparatus of meter need to occupy is larger, can not be directly installed on micro-fluidic chip so that current is micro-fluidic
Chip size is excessive.
Utility model content
In view of this, the utility model discloses a kind of micro-fluidic chip, micro-fluidic detection device and micro-fluidic detection sides
Method can effectively solve the detection sensitivity of traditional micro-fluidic chip and accuracy is low and the excessive technology of micro-fluidic detection device
Defect.
The utility model additionally provides a kind of micro-fluidic chip, including micro-fluidic chip substrate, determinand sensing chamber and side
Rubbing down optical fiber；The side throwing polishing fibre is fixed at the surface of the micro-fluidic chip substrate, the fine side of the side throwing polishing
Rubbing down area opening upwards are arranged on the surface of the micro-fluidic chip substrate；The determinand sensing chamber is wrapped in the side throwing mill
The periphery in the side throwing mill area of optical fiber so that determinand is placed in the side throwing mill area of the determinand sensing chamber.
Preferably, the inner surface embedded antibody or antigen in side throwing mill area, the antibody and determinand specificity knot
It closes；Or, the antigen is specifically bound with determinand.
Preferably, the inner surface in side throwing mill area covers liquid crystal layer, the liquid crystal layer closes for methacrylated
Object.
It should be noted that the liquid crystal layer material of the inner surface coating in side throwing mill area is methacrylate compound.Institute
Height-oriented, tilted alignment, twist alignment can be provided by stating liquid crystal layer, and the liquid crystal layer compound of coating can be to a certain range wavelength
Light wave carries out selective reflecting, is orientated to the calibration that light beam is oriented to reach, moreover it is possible to the light wave of a certain range wavelength
Selective deviation is carried out, calibration orientation is oriented to light beam to reach so that detection is sensitiveer.
Preferably, the micro-fluidic chip substrate is monocrystalline silicon piece, dimethyl silicone polymer, glass, quartz or poly- first
One kind in base methyl acrylate.
Preferably, micro-fluidic chip further includes plural sample transfer passage, the plural number sample transfer passage point
It is not connect with the determinand sensing chamber.
Preferably, the determinand sensing chamber further includes valve, the plural number sample transfer passage passes through described
Valve is connect with the determinand sensing chamber.
Preferably, the plural number sample transfer passage includes：Determinand transfer passage, negative sample transfer passage and
Positive sample transfer passage；The determinand transfer passage, the negative sample transfer passage and positive sample conveying are logical
Road is connect with the determinand sensing chamber respectively.
It should be noted that the side throwing that determinand transfer passage is used to determinand being transported to determinand sensing chamber grinds area
It is interior；Negative sample transfer passage is used to negative sample being transported in the side throwing mill area of determinand sensing chamber；Positive sample conveys
Channel is used to positive sample being transported in the side throwing mill area of determinand sensing chamber.
Preferably, micro-fluidic chip further includes sample transfer passage controller, the sample transfer passage controller with
The sample transfer passage connection so that the sample channel controller controls the determinand transfer passage, the negative sample
The flowing of the determinand, negative sample and positive sample of this transfer passage and the positive sample transfer passage.
More preferably, the sample transfer passage controller is connect with the valve of the determinand sensing chamber so that institute
It states sample transfer passage controller and controls the valve of the determinand sensing chamber and open or close.
It should be noted that the sample transfer passage controller control the valve of the determinand sensing chamber opening or
It closes, when the opening of the valve of the determinand sensing chamber, the sample transfer passage controller controls determinand described
Determinand sensing chamber is flowed, and information when determinand dynamic is detected；It is described when the closing of the valve of the determinand sensing chamber
Sample transfer passage controller controls determinand and detects indoor standing in the determinand, detects information when determinand static state,
Therefore, the micro-fluidic chip of the utility model can detect determinand dynamic or it is static when information.
Preferably, micro-fluidic chip further includes waste liquid reservoir part, the waste liquid reservoir part and the plural galley proof
Product transfer passage connects so that the waste liquid reservoir part stores determinand, negative sample and the sun of the determinand sensing chamber
Property sample.
The invention also discloses a kind of micro-fluidic detection device, including the micro-fluidic chip, data processor,
Luminous component and signal detecting part；The luminous component input terminal fine with the polishing of the side throwing of the micro-fluidic chip is connect,
The luminous component is used for the fine input terminal of the side throwing polishing to the micro-fluidic chip and sends out light source；The signal detecting part
The output end fine with the polishing of the side throwing of the micro-fluidic chip is connect, and the signal detecting part is for obtaining the micro-fluidic core
The optical signal of the fine output end of side throwing polishing of piece；The data processor respectively with the luminous component and the signal detection
Component connects, optical signal for obtaining the luminous component and the signal detecting part and to the luminous component and described
The optical signal of signal detecting part carries out analysis calculating.
Preferably, micro-fluidic detection device further includes display, the display is connect with the data processor, is used
In the result for showing that the data processor for analysis calculates.
It should be noted that data processor is obtained the optical signal of the luminous component and the signal detecting part
The ratio of optical signal.
It should be noted that optical signal is light intensity, spectrum or optical density.
More preferably, the fine side throwing mill area of the side throwing polishing is full rubbing down area or Fei Quan rubbing downs area.
It should be noted that full rubbing down area, i.e. optical fiber fibre core at rubbing down are blocked by rubbing down area completely, optical fiber leans on coat
It is connected with covering, the light that the input terminal fine from side throwing polishing transmits all leaks in full rubbing down area, the light warp leaked
Enter the fine output end of side throwing polishing after the determinand in Guo Quan rubbing downs area, the efficiency of transmission of light depends entirely on the property of determinand
And concentration；Fei Quan rubbing downs area, i.e. rubbing down area be not completely cut off by fibre core, and the optical fiber at Fei Quan rubbing downs area both ends is connected by fibre core
Come, the light part that the input terminal fine from side throwing polishing passes over is leaked from the rubbing down face in Fei Quan rubbing downs area, this portion
Point light enter in the fibre core of the rubbing down area other side by the determinand solution in rubbing down area, the light of another part is from rubbing down area bottom
Fibre core propagated, the light propagated by testing liquid solution can be detected simultaneously using non-full rubbing down optical fiber and by rubbing down area
The light that bottom fibre core is propagated, can compare this two parts light, obtain the property and concentration data of determinand solution.
The utility model is found, side throwing is ground fiber optic applications in microflow control technique, can effectively solve microflow control technique, by
It is that ordinary optic fibre covering rubbing down is fallen optical fiber made of a part using optics micro-processing technology to polish fibre in side throwing, when optical fiber
When membrane wrapping thickness is reduced to region existing for evanscent field due to rubbing down processing, the window of a fibre core light field, Ke Yili can be formed
With evanscent field come excite, control, the propagation light in detection optical fiber fibre core, ordinary optic fibre has of low cost, soft easy processing
Characteristic, and the region that evanscent field utilizes can artificially be controlled, back-reflection is small, is easy to and fibre system welding.Therefore, this practicality
It is novel creatively to use the fine core element as micro-fluidic detection device of side throwing polishing.Side throwing polishing is fine to have soft, light
The characteristic that Louis is adjusted, micro-fluidic detection device is high with detection sensitivity made of side throwing polishing fibre, accuracy is high, volume
Small advantage can be incorporated on micro-fluidic chip.The utility model uses the fine core as micro-fluidic detection device of side throwing polishing
The fine rubbing down face of side throwing polishing is used as " window " of detection determinand by center portion part, allows determinand through the fine side throwing of side throwing polishing
Area is ground, the light that light source is sent out is transmitted to when side throwing grinds area through side throwing polishing fibre and leaks, and the light of leakage is through the to be measured of rubbing down face
Object is propagated after propagating through the fine output end of side throwing polishing, and can be detected by signal detecting part and be received optical signal.Passing through will
It is compared by the optical signal of the fine input terminal of side throwing polishing and the optical signal of the fine output end of side throwing polishing, it can be deduced that quilt
Survey the property and concentration of object.
The purpose of this utility model is for the detection sensitivity of traditional micro-fluidic chip and accuracy are low in the prior art
Excessive with micro-fluidic detection device, therefore, the utility model discloses a kind of micro-fluidic chip, including micro-fluidic chip substrate, waits for
It surveys analyte detection room and side throwing polishing is fine；Side throwing polishing fibre is fixed at the surface of micro-fluidic chip substrate, side throwing polishing fibre
Side throwing mill area's opening upwards are arranged on the surface of micro-fluidic chip substrate；Determinand sensing chamber is wrapped in the fine side throwing of side throwing polishing
Grind the periphery in area so that determinand is placed in the side throwing mill area of determinand sensing chamber；Micro-fluidic detection device, including micro-fluidic core
Piece, data processor, luminous component and signal detecting part；The fine input terminal of the side throwing of luminous component and micro-fluidic chip polishing
Connection, luminous component are used for the fine input terminal of the side throwing polishing to micro-fluidic chip and send out light source；Signal detecting part and miniflow
The side throwing for controlling chip polishes fine output end connection, and signal detecting part is used to obtain the defeated of the side throwing polishing fibre of micro-fluidic chip
The optical signal of outlet；Data processor is connect with luminous component and signal detecting part respectively, for obtaining luminous component and letter
The optical signal of number detection part simultaneously carries out analysis calculating to the optical signal of luminous component and signal detecting part.
When in use, the side throwing for determinand, negative sample and positive sample being individually placed in determinand sensing chamber is ground
In area, the input terminal of luminous component offside rubbing down optical fiber sends out light source, and signal detecting part detects the side throwing mill of micro-fluidic chip
The optical signal of the output end of optical fiber, the input terminal of offside rubbing down optical fiber and the optical signal of output end are compared, and are polished from side throwing
Fine input terminal is propagated through the light come and spreads into the fine output end of side throwing polishing after the determinand in rubbing down area, and then is believed
Number detection part detects.
In some embodiments, for the quantitative analysis of determinand, the concentration of the determinand in rubbing down area is higher, side throwing mill
The ratio for the optical signal that the fine input terminal of optical signal and side throwing that the output end of optical fiber detects polishing detects is smaller, by with
The optical signal ratio of master sample compares, and can obtain the concentration of determinand solution.Utilize calculation formula：Testing concentration=side
The output end optical signal of the rubbing down optical fiber/fine input terminal optical signal × concentration factor of side throwing polishing.Wherein, optical signal be light intensity,
Spectrum or optical density.By formulating the standard curve of determinand, the positive control and negative control sample of determinand
Standard curve preparation method：Pass through the input of the positive control of the negative control sample and determinand of determinand
The relationship of output intensity ratio and concentration establishes standard curve, then detects the side throwing polishing of determinand fine input terminal and output
The light intensity ratio at end calculates the concentration of determinand by standard curve, wherein the negative control sample of determinand be without containing
The positive control of the solvent of determinand, determinand is the solvent containing normal concentration determinand.The concentration of different determinands
Coefficient determination is the gradient concentration dilution by preparing determinand, measures various concentration determinand dilution in miniflow of the present invention
The light intensity for controlling the input terminal and output end of detection device is that x values, the determinand are dense with the light intensity ratio of the input terminal and output end
Degree is that y values draw standard curve, and testing concentration coefficient is slope of standard curve.
In some embodiments, for the qualitative analysis of the low determinand of content, concentration of the utility model to determinand
It is amplified, to improve limit and the sensitivity of detection.The antibody or antigen of the inner surface in area, determinand and packet are ground by side throwing
Combined by the antigen or antibody that grind the inner surface in area in side throwing, after unbonded Impurity elution, addition can be coated on side throwing
It grinds the antigen of the inner surface in area or antibody combines and the secondary antibody with fluorophor.Fluorophor in secondary antibody is through light
Fluorescence is sent out after the light excitation for the specific wavelength that source is sent out, is detected by signal detecting part, and the concentration of determinand is divided
Analysis.The brightness of fluorescence is directly proportional to the concentration of test substance, and the concentration information of determinand can be amplified by fluorescence, therefore can root
The concentration of determinand is calculated according to the brightness of the fluorescence of detection, therefore, the utility model can carry out the concentration of determinand special
Property Fluorescence amplification reaction.
In some embodiments, for the qualitative analysis of the low determinand of content, concentration of the utility model to determinand
It is amplified, to improve limit and the sensitivity of detection.It is detected after being amplified by the characteristic of layer of liquid crystal molecule to determinand.
Layer of liquid crystal molecule is coated on the fine side throwing of side throwing polishing and grinds area, before determinand addition, layer of liquid crystal molecule is in tiled state, light
The luminous energy that source is sent out successfully passes through side throwing mill area to reach the fine output end of side throwing polishing, is detected by the detector.It is added to be measured
After object, orderly change occurs for the arrangement mode of liquid crystal molecule, can influence the passability of light, can be added by detecting test substance
Front and back light changes to be measured to the concentration of test substance.Test substance of the variation of Liquid Crystal Molecules Alignment mode to addition
Amount it is very sensitive, micro substance all can make the arrangement mode of liquid crystal molecule that orderly change occur, and influence the passability of light, because
This this method can play the role of the concentration information of test substance being amplified.
It should be noted that plural sample transfer passage is equipped with determinand injection port, negative sample pipeline is equipped with feminine gender
Sample injection port, positive sample pipeline are equipped with positive sample injection port, and determinand, negative sample and positive sample are respectively from correspondence
Injection port injection, first carry out the detection of negative sample, followed by the detection of positive sample is finally the detection of determinand.Hair
The fine input terminal of side throwing polishing of the light component to micro-fluidic chip emits light source, and the transmission in area is ground through side throwing, reaches side throwing polishing
Fine output end, signal detecting part detect the optical signal of the fine output end of side throwing polishing, can obtain the information for beating determinand.
It should be noted that the utility model can detect uric acid, nucleic acid (including DNA and RNA), organic or inorganic object.
A kind of micro-fluidic chip disclosed by the utility model has the advantages that：The micro-fluidic chip of the utility model
Light path is simple, detection speed is fast, setting side throwing grinds area and makes strong antijamming capability, testing result accurate；Due to the letter of its structure
It is single, small, therefore, the utility model manufacturing cost is low, it is easy to carry, easy to operate, can at the scene or family uses.From this
The embodiment of utility model it is found that micro-fluidic detection device is used for the detection of actual determinand, either directly to determinand into
Row detection, or the amplified detection of chromogenic reaction is carried out to determinand, all obtain accurate detection result, the standard of testing result
True property is with spectrophotometer without significant difference (P>0.05), illustrate that the detection sensitivity of the utility model is high, accuracy is high, volume
It is small and easy to carry.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described.
Fig. 1 shows a kind of schematic diagram figure of micro-fluidic chip provided by the utility model；
Fig. 2 shows a kind of structure sectional view of micro-fluidic chip provided by the utility model；
Fig. 3 shows a kind of internal structure chart of micro-fluidic detection device provided by the utility model；
Fig. 4 shows a kind of plane structure chart of micro-fluidic detection device provided by the utility model
Fig. 5 shows that the utility model provides the standard of the concentration and input and output light intensity ratio of the NaCl solution of micro-fluidic chip
Curve；
Fig. 6 shows that the utility model provides the continuous spectrum stacking chart of the pure water and serum of micro-fluidic chip；
Fig. 7 shows that the utility model provides the continuous spectrum figure of the pure water of micro-fluidic chip；
Fig. 8 shows that the utility model provides the continuous spectrum figure of the serum of micro-fluidic chip；
Fig. 9 shows that the utility model provides the spy of cell genomic dna extracted in the HBE cells in the people source of micro-fluidic chip
Levy absorption peak stacking chart；
Wherein, reference numeral, micro-fluidic chip substrate 1, side throwing mill area 2, side throwing polishing fibre 3, determinand sensing chamber 4, hair
Light component 5, signal detecting part 6, processor 7, display 8, sample transfer passage 9, waste liquid reservoir part 10, side throwing polishing are fine
Input terminal A, the fine output end B of side throwing polishing.
Specific implementation mode
The utility model provides a kind of micro-fluidic chip and micro-fluidic detection device, for solving traditional micro-fluidic core
The detection sensitivity of piece and accuracy is low and the excessive technological deficiency of micro-fluidic detection device.
Below by the technical scheme in the utility model embodiment is clearly and completely described, it is clear that described
Embodiment is only the utility model a part of the embodiment, instead of all the embodiments.Based on the implementation in the utility model
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The range of the utility model protection.
Embodiment 1
It please refers to Fig.1 to Fig.4, the micro-fluidic chip of the present embodiment includes micro-fluidic chip substrate 1, determinand sensing chamber 4
With side throwing polishing fibre 3；Side throwing polishing fibre 3 is fixed at the surface of micro-fluidic chip substrate 1, the side throwing mill of side throwing polishing fibre 3
2 opening upwards of area are arranged on the surface of micro-fluidic chip substrate 1；Determinand sensing chamber 4 is wrapped in the side throwing mill of side throwing polishing fibre 3
The periphery in area 2 so that determinand is placed in the side throwing mill area of determinand sensing chamber 4.
Further, the inner surface embedded antibody or antigen in side throwing mill area 2, antibody are specifically bound with determinand；Or, anti-
It is former to be specifically bound with determinand.
Further, the inner surface in side throwing mill area 2 covers liquid crystal layer, and liquid crystal layer by side throwing for that will grind the light source in area 2
Focus on the fine center of 3 side throwings polishing.
Further, micro-fluidic chip substrate 1 is monocrystalline silicon piece, dimethyl silicone polymer, glass, quartz or poly- methyl-prop
One kind in e pioic acid methyl ester.
Further, micro-fluidic chip further includes plural sample transfer passage 9, and plural number sample transfer passage 9 is distinguished
It is connect with determinand sensing chamber 4.
Further, determinand sensing chamber 4 further includes valve, plural sample transfer passage 9 by valve with it is to be measured
Analyte detection room 4 connects.
Further, a plural sample transfer passage 9 includes：Determinand transfer passage, negative sample transfer passage and sun
Property sample transfer passage；Determinand transfer passage, negative sample transfer passage and positive sample transfer passage respectively with determinand
Sensing chamber 4 connects.
Further, further include sample transfer passage controller, sample transfer passage controller and the company of sample transfer passage 9
It connects so that sample channel controller controls determinand transfer passage, negative sample transfer passage and positive sample transfer passage
The flowing of determinand, negative sample and positive sample.
Further, sample transfer passage controller is connect with the valve of determinand sensing chamber so that sample transfer passage
The valve of controller control determinand sensing chamber opens or closes.
Further, further include waste liquid reservoir part 10, waste liquid reservoir part 10 and the plural company of sample delivery channel 9
It connects so that waste liquid reservoir part 10 stores determinand, negative sample and the positive sample of determinand sensing chamber 4.
The present embodiment also discloses a kind of micro-fluidic detection device, including micro-fluidic chip, data processor, luminous component
5 and signal detecting part 6；The input terminal A fine with the polishing of the side throwing of micro-fluidic chip of luminous component 5 is connect, and luminous component 5 is used for
The fine input terminal A of side throwing polishing to micro-fluidic chip sends out light source；Signal detecting part 6 and the side throwing of micro-fluidic chip are polished
Fine output end B connections, signal detecting part 6 are used to obtain the optical signal of the fine output end B of side throwing polishing of micro-fluidic chip；
Data processor is connect with luminous component 5 and signal detecting part 6 respectively, for obtaining luminous component 5 and signal detecting part 6
Optical signal and analysis calculating is carried out to the optical signal of luminous component 5 and signal detecting part 6.
Further, further include display, display 8 is connect with data processor, for display data processor analysis
The result of calculating.
Embodiment 2
In the present embodiment, determinand is the NaCl solution prepared at random, and negative control sample is pure water, positive control sample
This is the NaCl solution of known concentration.
Table 1 makes standard curve using the NaCl solution of various concentration
Fine input terminal is polished using the side throwing of micro-fluidic chip disclosed by the utility model and side throwing polishes fibre respectively
The ratio of the light intensity of output end is converted into concentration value according to standard curve, is the concentration ratio of the determinand of spectrophotometric determination
Right, experiment is repeated 3 times, and testing result is as shown in table 1- tables 2 and Fig. 5.
The testing result for the NaCl solution prepared at random that 2 micro-fluidic chip of table is measured with spectrophotometric
Conclusion：The measurement result of the utility model micro-fluidic chip is similar to the measurement result of spectrophotometer, P>0.05.
Embodiment 3
It is pure that the present embodiment, which uses Quality Control genome sample (0.01g/L) to be used as positive control, negative control sample,
Water, determinand be 5million people source HBE cells in the cell genomic dna that extracts.
Referring to Fig. 9, negative sample, positive sample and each 1 μ l of determinand to be separately added by sample transfer passage 9 and be waited for
It surveys in the side throwing mill area 2 of analyte detection room 4, luminous component emits light source in the fine input terminal of the side throwing polishing of micro-fluidic chip,
Light source is the light source of 260nm absorbing wavelengths, and fine output end is polished by the light intensity and side throwing of the fine input terminal of side throwing polishing
Light intensity draws standard curve.The detection of negative control sample is first carried out, the concentration value of negative control sample is shown in liquid crystal display
On screen；Then the detection of positive control is carried out, the concentration value of positive control is shown on liquid crystal display.This practicality
The novel average detected time from bringing into operation display density value is 3.2s.Positive control is Quality Control genome sample
(0.01g/L), negative control sample are pure water, and determinand (cell genomic dna extracted in the HBE cells in people source) is equally logical
It crosses spectrophotometer to be detected, the use of spectrophotometer is according to manufacturer's operating instruction, when the average detected of spectrophotometer
Between be 4.1s.Negative control sample, positive control and determinand carry out 3 detections, then average statistical and standard deviation,
And the measured value that side throwing is polished to fine micro-fluidic chip is compared with the measured value of spectrophotometer.
The testing result for the pure water that 3 micro-fluidic chip of table is measured with spectrophotometric
The testing result for the Quality Control genome sample that 4 micro-fluidic chip of table is measured with spectrophotometric
The cell genomic dna extracted in the HBE cells in the people source that 5 micro-fluidic chip of table is measured with spectrophotometric
Testing result
Conclusion：The utility model micro-fluidic chip and spectrophotometer are in the detection to sample of nucleic acid, two kinds of detection
As a result close, no significant difference (P>0.05).
Embodiment 4
The present embodiment uses uric acid titer (0.05g/L) to be used as positive control, and negative control sample is pure water, is waited for
Survey object is serum.Negative control sample, positive control and determinand carry out 3 detections, then average statistical and standard
Difference, and the measured value of the fine micro-fluidic chip of side throwing polishing is compared with the measured value of spectrophotometer.
Determinand is before detection with after the first detection reagent hybrid reaction of 5 μ l, and determinand is being placed in this after being handled
The micro-fluidic chip of utility model is detected, and the first detection reagent is iron (III) solution and Phen solution, iron (III)
A concentration of 20mg/L, a concentration of 1g/L of Phen, iron (III) solution and Phen solution are with 1:1 ratio mixing.
Detection wavelength selects 510nm to give tacit consent to wavelength, first carries out the detection of negative control sample, the concentration of negative control sample
Value display is over the display；Then the detection of positive control is carried out, the concentration value of positive control is shown in display
On.Average detected time of the utility model from bringing into operation display density value is 3.2s, spectrophotometer (upper Nereid section
Average detected time 721G) is 4.2s.
6 micro-fluidic chip of table and the testing result for the pure water that spectrophotometric measures are as follows
The continuous spectrum figure for the pure water that micro-fluidic chip measures is as shown in Figure 7.
7 micro-fluidic chip of table and the testing result for the uric acid titer (0.05g/L) that spectrophotometric measures are as follows
8 micro-fluidic chip of table and the Virus monitory result that spectrophotometric measures are as follows
The continuous spectrum figure for the serum that micro-fluidic chip measures is as shown in Figure 8.
Conclusion：The measurement result of the utility model micro-fluidic chip and the measurement result of spectrophotometer, no significant difference
(P>0.05)。
Embodiment 5
The present embodiment uses uric acid titer (0.05g/L) to be used as positive control.Negative control sample is pure water, is waited for
Survey object is serum.Positive control and determinand carry out 3 detections, then average statistical and standard deviation, and side throwing is ground
The measured value of optical fibre micro-fluidic chip is compared with the measured value of spectrophotometer.
Determinand is before detection with after the second detection reagent hybrid reaction of 5 μ l, and determinand is being placed in this after being handled
The micro-fluidic chip of utility model is detected, and the second detection reagent is urate oxidase, peroxidase (POD), 4- amino
Tri- bromo- 3- hydroxybenzoic acids (TBHBA) of antipyrine (4-AAP) and 2,4,6-.Uric acid can be oxidized to hydrogen peroxide by uricase
And allantoin, in the presence of peroxidase (POD), hydrogen peroxide oxidation 4-AA (4-AAP) and 2,4,6-
Three bromo- 3- hydroxybenzoic acids (TBHBA), reaction generate product red quinone imines, can cause the upper of absorbance at 520nm wavelength
It rises, wherein the second detection reagent is configured using existing method.
Detection wavelength selects 510nm to give tacit consent to wavelength, first carries out the detection of negative control sample, the concentration of negative control sample
Value is shown on liquid crystal display；Then the detection of positive control is carried out, the concentration value of positive control is shown in liquid
On crystal display screen.
The testing result for the pure water that 9 micro-fluidic chip of table is measured with spectrophotometric
The testing result for the uric acid titer (0.05g/L) that 10 micro-fluidic chip of table is measured with spectrophotometric
The testing result for the serum that 11 micro-fluidic chip of table is measured with spectrophotometric
The continuous spectrum stacking chart for the pure water and serum that micro-fluidic chip measures is as shown in Figure 6.
Conclusion：The measurement result of the utility model micro-fluidic chip is close with the measurement result of spectrophotometer, without significantly
Difference (P>0.05)
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and
Retouching also should be regarded as the scope of protection of the utility model.
Claims (12)
1. a kind of micro-fluidic chip, which is characterized in that polished including micro-fluidic chip substrate, determinand sensing chamber and side throwing fine；
The side throwing polishing fibre is fixed at the surface of the micro-fluidic chip substrate, and the fine side throwing of the side throwing polishing grinds area
Opening upwards are arranged on the surface of the micro-fluidic chip substrate；
The determinand sensing chamber is wrapped in the periphery in the fine side throwing mill area of the side throwing polishing so that determinand is placed in described wait for
The side throwing for surveying analyte detection room grinds area.
2. micro-fluidic chip according to claim 1, which is characterized in that the inner surface embedded antibody in side throwing mill area or
Antigen, the antibody are specifically bound with determinand；
Alternatively, the antigen is specifically bound with determinand.
3. micro-fluidic chip according to claim 1, which is characterized in that the inner surface in side throwing mill area covers liquid crystal
Layer, the liquid crystal layer are methacrylate compound.
4. micro-fluidic chip according to claim 1, which is characterized in that the micro-fluidic chip substrate be monocrystalline silicon piece,
One kind in dimethyl silicone polymer, glass, quartz or polymethyl methacrylate.
5. micro-fluidic chip according to claim 1, which is characterized in that further include plural sample transfer passage, it is described
Plural sample transfer passage is connect with the determinand sensing chamber respectively.
6. micro-fluidic chip according to claim 5, which is characterized in that the determinand sensing chamber further includes valve, institute
Plural sample transfer passage is stated to connect with the determinand sensing chamber by the valve.
7. micro-fluidic chip according to claim 6, which is characterized in that it is described plural number a sample transfer passage include：It waits for
Survey object transfer passage, negative sample transfer passage and positive sample transfer passage；The determinand transfer passage, the negative sample
This transfer passage and the positive sample transfer passage are connect with the determinand sensing chamber respectively.
8. micro-fluidic chip according to claim 7, which is characterized in that further include sample transfer passage controller, it is described
Sample transfer passage controller is connect with the sample transfer passage so that is waited for described in the sample transfer passage controller control
Survey determinand, negative sample and the sun of object transfer passage, the negative sample transfer passage and the positive sample transfer passage
The flowing of property sample.
9. micro-fluidic chip according to claim 8, which is characterized in that the sample transfer passage controller is waited for described
Survey the valve connection of analyte detection room so that the sample transfer passage controller controls beating for the valve of the determinand sensing chamber
On or off is closed.
10. micro-fluidic chip according to claim 8, which is characterized in that further include waste liquid reservoir part, the waste liquid storage
It deposits component to connect with the plural sample delivery channel so that the waste liquid reservoir part stores the determinand sensing chamber
Determinand, negative sample and positive sample.
11. a kind of micro-fluidic detection device, which is characterized in that include micro-fluidic as described in claims 1 to 10 any one
Chip, data processor, luminous component and signal detecting part；
The luminous component input terminal fine with the polishing of the side throwing of the micro-fluidic chip is connect, and the luminous component is used for institute
The input terminal for stating the side throwing polishing fibre of micro-fluidic chip sends out light source；
The signal detecting part output end fine with the polishing of the side throwing of the micro-fluidic chip is connect, the signal detecting part
Side throwing for obtaining the micro-fluidic chip polishes the optical signal of fine output end；
The data processor is connect with the luminous component and the signal detecting part respectively, for obtaining the illumination region
The optical signal of part and the signal detecting part simultaneously divides the optical signal of the luminous component and the signal detecting part
Analysis calculates.
12. micro-fluidic detection device according to claim 11, which is characterized in that further include display, the display
It is connect with the data processor, the result calculated for showing the data processor for analysis.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109580506A (en) * | 2018-11-28 | 2019-04-05 | 天津瑞生物科技股份有限公司 | A kind of fungal detection system and two Methods for Fungi Detection based on digital microfluidic technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109580506A (en) * | 2018-11-28 | 2019-04-05 | 天津瑞生物科技股份有限公司 | A kind of fungal detection system and two Methods for Fungi Detection based on digital microfluidic technology |
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