CN208038466U - A kind of small-sized cell culture unit of pressure controllable - Google Patents
A kind of small-sized cell culture unit of pressure controllable Download PDFInfo
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- CN208038466U CN208038466U CN201820247585.0U CN201820247585U CN208038466U CN 208038466 U CN208038466 U CN 208038466U CN 201820247585 U CN201820247585 U CN 201820247585U CN 208038466 U CN208038466 U CN 208038466U
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- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 230000007246 mechanism Effects 0.000 claims description 33
- 238000009434 installation Methods 0.000 claims description 10
- 238000012549 training Methods 0.000 claims description 9
- 230000005540 biological transmission Effects 0.000 claims description 8
- 239000012780 transparent material Substances 0.000 claims description 4
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000004807 localization Effects 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 12
- 208000010412 Glaucoma Diseases 0.000 abstract description 5
- 238000011160 research Methods 0.000 abstract description 4
- 230000006872 improvement Effects 0.000 description 20
- 210000004027 cell Anatomy 0.000 description 16
- 238000010586 diagram Methods 0.000 description 8
- 230000008859 change Effects 0.000 description 5
- 230000007613 environmental effect Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
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- 230000001360 synchronised effect Effects 0.000 description 2
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- 238000004891 communication Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 210000002592 gangliocyte Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
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Abstract
The utility model discloses a kind of small-sized cell culture units of pressure controllable, including incubator, adjustable air pump, the first adjustable valve, the second adjustable valve, pressure gauge and third adjustable valve, incubator includes babinet, multiple culture spaces for being set in babinet and the case lid for being set to box opening；Adjustable air pump connect after the first adjustable valve by it is multiple enter pipeline connect one to one respectively in multiple culture spaces, each enter and externally connect bye-pass on pipeline and the second adjustable valve is set on bye-pass；Each equal external connection of culture space is arranged pressure gauge, each culture space by it is multiple go out pipeline external connection, and it is multiple go out pipeline collect and connect third adjustable valve.The utility model can carry out the control experiment of different pressures in multiple culture spaces, and the experimental situation of multiple culture spaces can be kept consistent, obtain accurate experimental result, be more advantageous to the influence that research high pressure falls ill to glaucoma.
Description
Technical field
The utility model is related to medical experiment field more particularly to a kind of small-sized cell culture units of pressure controllable.
Background technology
Glaucoma is a kind of irreversible damage with retinal ganglial cells (retinal ganglion cells, RGCs)
Wound is the neurodegenerative disease of pathological characters, is the ophthalmology disease of global blind rate first.Global 40 to 80 years old age bracket blueness
There are about 64,300,000 people by light eye patient, it is contemplated that is up to 76,000,000 people and 100,000,000 1,180 ten thousand people respectively to the year two thousand twenty and the year two thousand forty.Pathology
Property intraocular pressure raising be cause glaucoma occur and progress an important factor for, influence of the in-vitro simulated high pressure to relevant cell is
In the important component of glaucoma morbidity making and research.
Cells in vitro pressure culture is to apply a kind of cell culture skill of different pressures in incubation to cell in vitro
Art, to study influence of the pressure to characteristics of cell biology.Pressure is the important factor in order of cell growth, proliferation, lesion,
In the experiment of cell injuring model, often needs to simulate different pressure environments, study under different pressures, cell and intracellular chemistry
The variation of substance.Many diseases be all as the change of cells survival environmental pressure and caused by, in order to study the hair of such disease
Interpretation of the cause, onset and process of an illness system, we often need to simulate this abnormal pressure environment.
But in existing pressure culture, Technical comparing is single, generally directly uses inflating ball and pressure gauge joint threeway
Pipe is inflated into culture bottle, and disadvantage mainly has at 2 points：The circulation that gas is kept while 1. cannot accomplish to maintain air pressure, with
The progress of cell culture, the CO2 concentration in culture bottle, pH value etc. can have greatly changed, can not situation completely in analogue body；
2. operating complex, in order to create suitable condition, self reliant rebuilding animal pressurizes storehouse to make culture instrument in some laboratories
The time energy of device, consuming is larger.Therefore, novel culture device is developed to facilitating experiment, and then pushes the progress of research
Just it is particularly important.
Utility model content
In view of the deficienciess of the prior art, the purpose of this utility model is to provide a kind of simple in structure, provide mostly empty
Between carry out cells in vitro pressure experiment, and can guarantee the unified small-sized cell culture unit of its dependent variable.
In order to solve the above technical problems, the utility model uses following technical scheme：A kind of minicell of pressure controllable
Culture apparatus, including incubator, adjustable air pump, the first adjustable valve, the second adjustable valve, pressure gauge and third adjustable valve, incubator
Including babinet, the multiple culture spaces being set in babinet and the case lid for being set to box opening；Adjustable air pump connection first
After adjustable valve by it is multiple enter pipeline connect one to one respectively in multiple culture spaces, each enter on pipeline and externally connect
Simultaneously the second adjustable valve is arranged in bye-pass on bye-pass；Pressure gauge is arranged in each equal external connection of culture space, and each culture is empty
Between by it is multiple go out pipeline external connection, and it is multiple go out pipeline collect and connect third adjustable valve.
As an improvement enter pipeline and go out the pipeline two side that be respectively arranged at babinet opposite, pressure gauge and go out pipeline
It is arranged in homonymy.
As an improvement multiple culture spaces are from top to bottom arranged, case lid is opened in the side of babinet, incubator
In be provided with rotating mechanism, dismountable culture tray is arranged in multiple culture spaces, and several culture dishes are placed in culture tray, revolves
Rotation mechanism drives culture tray to be rotated at work.
As an improvement rotating mechanism includes motor, shaft, plane bearing and driving cog, motor is set to babinet
Bottom, the upper lower wall that shaft extends upward through culture space from motor penetrate multiple culture spaces, each culture space
Plane bearing is set on lower wall, which is sheathed in shaft, and driving cog is arranged on plane bearing, which is sheathed on
It links in shaft and with shaft；Culture tray is disk and has a mounting groove, and mounting groove is up and down through culture tray and from training
It supports pallet side and extends to axle center, the center of the lower surface of culture tray is provided with the kerve coordinated with driving cog, kerve
Cell wall there is the internal tooth of transmission of being meshed with driving cog.
As an improvement rotating mechanism includes motor, shaft and plane bearing, motor is set to the bottom of babinet, turns
The upper lower wall that axis extends upward through culture space from motor penetrates multiple culture spaces, is set on the lower wall of each culture space
Horizontalization spherical bearing is used to support culture tray, which is sheathed in shaft, and side channel and jack are provided in shaft；Culture
Pallet is disk and there is a mounting groove, mounting groove through culture tray and to extend to axle center from culture tray side up and down, is pacified
The slot bottom of tankage has inserting column, and installation, side channel is coordinated to limit culture tray, peace at the mounting groove of culture tray and the side channel of shaft
Inserting column is inserted into jack when being attached to.
As an improvement rotating mechanism includes motor, shaft, plane bearing and driving band, case lid has installation cavity, electricity
Machine is set to installation cavity bottom, and shaft upwardly extends setting from motor, setting connection corresponding with culture space quantity in shaft
Dynamic driving band, driving band part stretch out the side wall of case lid；Setting plane bearing is used to support on the lower wall of each culture space
Culture tray, when Box Cover upper box lid, stretch out case lid driving band be in contact with the culture tray in culture space cooperation pass
It is dynamic.
As an improvement the lower surface of culture tray is provided with kerve, kerve matches with plane bearing.
As an improvement being provided with circumferentially evenly distributed several columns in culture tray, confession is provided on column
The holding tank that culture dish is placed and the detent mechanism being set on the outside of holding tank, when culture dish is placed into holding tank by fixed
Position mechanism limits culture dish.
As an improvement detent mechanism includes regulation ring, pushing block and limited block, regulation ring has an internal thread, outside column
Week has external screw thread, and regulation ring is mobilizable to be threaded onto on column, and column periphery is axially provided with the first sliding rail, first cunning
Rail extends to column upper surface, and column upper surface is corresponding with the first sled position is provided with the second radial sliding rail, pushing block
It is installed on above regulation ring by the way that the first sliding block being arranged thereon and the cooperation of the first sliding rail are mobilizable, limited block by setting thereon
The second sliding block set and the second sliding rail cooperation it is mobilizable be installed on column upper surface, pass through angled-lift splits between pushing block and limited block
With being equipped with for oblique sliding rail slip, the rubber layer with culture dish cooperation positioning is provided on limited block；When regulation ring rotates
When rising, pushing block is driven to rise and limited block linkage inwardly sliding.
As an improvement the one side or/and case lid of babinet are set as transparent material.
The advantageous effect that the utility model reaches is：Lacking for single pressure can only be maintained by breaching original cell incubator
It falling into, original cell incubator is closed culture, with the passage of incubation time, PH, P, CO in incubator2、PO2Can it occur
Change, influences the growth of cell；The utility model enables gas to circulate, the environment of more preferable simulation cell real-life,
Reach and kept gas flowing while High pressure culture, has made gas temperature inside and outside cell culture apparatus, humidity, CO2Concentration one
It causes.Adjustable air pump provides air pressure, and can ensure that experimental situation is consistent with external environment, can by the first adjustable valve and third
It adjusts valve in access control integral pressure, the pressure of each culture space is controlled by several second adjustable valves, pressure gauge enables experimenter
Intuitive decompression force, can carry out the control experiment of different pressures, and can keep multiple cultures skies in multiple culture spaces
Between experimental situation it is consistent, obtain accurate experimental result, be more advantageous to the influence fallen ill to glaucoma of research high pressure.Size
What can be designed is smaller, and cell culture apparatus is placed into closed big incubator to control other environmental conditions, improves dress
Set the convenience used and adaptability.
Description of the drawings
Fig. 1 is the structure connection diagram of the small-sized cell culture unit of the utility model.
Fig. 2 is the dimensional structure diagram of the incubator of the utility model.
Fig. 3 be the utility model incubator in rotating mechanism the first embodiment cross-section structure to regarding signal
Figure.
Fig. 4 is the overlooking structure diagram of the first embodiment of the culture tray of the utility model.
Fig. 5 be the utility model incubator in rotating mechanism second of embodiment cross-section structure to regarding signal
Figure.
Fig. 6 is the overlooking structure diagram of second of embodiment of the culture tray of the utility model.
Fig. 7 be the utility model incubator in rotating mechanism second of embodiment cross-section structure to regarding signal
Figure.
Fig. 8 is the enlarged structure schematic diagram at M in Fig. 7.
Fig. 9 is the overlooking structure diagram of the third embodiment of the culture tray of the utility model.
Figure 10 be the detent mechanism of culture dish in the culture tray of the utility model cut-away section in the first state and
Perspective structure schematic diagram.
Figure 11 be the detent mechanism of culture dish in the culture tray of the utility model cut-away section in the second condition and
Perspective structure schematic diagram.
Specific implementation mode
The utility model will be further described with reference to the accompanying drawings and detailed description.
As shown in figs. 1-11, the embodiment of the small-sized cell culture unit of pressure controllable provided by the utility model, the reality
It includes incubator 1, adjustable air pump 2, the first adjustable valve 3, the second adjustable valve 4, pressure gauge 5 and third adjustable valve 6, incubator to apply example
1 includes babinet 11, the multiple culture spaces 12 being set in babinet 11 and the case lid 13 for being set to 11 opening of babinet；It can regulating the qi flowing in the channels
After 2 the first adjustable valves 3 of connection of pump by it is multiple enter pipeline 7 connect one to one respectively in multiple culture spaces 12, each enter
Bye-pass 9 is externally connected on pipeline 7 and the second adjustable valve 4 is set on bye-pass 9；12 equal external connection of each culture space
Be arranged pressure gauge 5, each culture space 12 by it is multiple go out 8 external connection of pipeline, and it is multiple go out pipeline 8 collect and connect third
Adjustable valve 6.
When the utility model is used, can be positioned in existing incubator, environment is adjusted by small space
Variable is convenient for reference to experiment.Adjustable air pump 2 draws external gas, enters pipeline 7 by the first adjustable valve 3, first can
Valve 3 is adjusted to carry out whole control to the air pressure of air inlet；Entering pipeline 7 divides more to connect one to one into culture space 12, then is entering
Pipeline 7 picks out several second adjustable valves 4, and by adjusting the aperture in different second adjustable valves 4, realization is passed through different culture spaces
12 air pressure is different, is adjusted to carry out the control experiment of different pressures under same environment as required.Pressure value is logical
Excess pressure table 5 is read, and experimenter intuitively can control and adjust, go out pipeline 8 and collect connection third adjustable valve 6, with
First adjustable valve 3 is coordinated, and to control whole pressure, keeps the pressure stability in culture space 12.Case lid 13 is closed in lid
Later, multiple culture spaces 12 are closed, culture space 12 is only in communication with the outside by entering pipeline 7 and going out pipeline 8, and can regulating the qi flowing in the channels
Pump 2 be used as inlet, carry out being passed through for gas, the gas for being passed through each culture space 12 is consistent, enable integral experiment in addition to
Environment having the same except pressure is conducive to obtain accurate experimental data；Breaching original cell incubator can only maintain
The defect of single pressure, original cell incubator is closed culture, with the passage of incubation time, PH, P in incubator,
CO2、PO2It can change, influence the growth of cell；The utility model enables gas to circulate, and more preferable simulation cell is true
Grow directly from seeds environment living, has reached and has kept gas flowing while High pressure culture, has made each parameter of gas inside and outside cell culture apparatus
Unanimously.
As an improvement specific implementation mode, enter pipeline 7 and go out pipeline 8 and be respectively arranged at the opposite both sides of babinet 11
Wall, pressure gauge 5 are arranged with pipeline 8 is gone out in homonymy.Arrangement above mode enables the flowing of gas be full of entire culture space 12, gas
It is flowed into from side, other side outflow, pressure is uniform and stable in whole holding culture space 12；Go out 8 side of pipeline as gas stream
Dynamic outlet side has more accurate real-time pressure relative to entrance side, monitors and control convenient for experimenter.
As an improvement specific implementation mode, multiple culture spaces 12 are from top to bottom arranged, and case lid 13 opens up
In the side of babinet 11, rotating mechanism 0 is provided in incubator 1, dismountable culture tray 14 is arranged in multiple culture spaces 12,
Several culture dishes 15 are placed in culture tray 14, rotating mechanism 0 drives culture tray 14 to be rotated at work.Culture space
Air-flow in 12 is flowed, and because of the fixation of entrance, the air-flow environment that different location can keep certain will not change, culture dish
15 might have a little environmental difference in different location, therefore rotating mechanism 0 is arranged, and culture tray 14 is enabled to be driven to rotate, that
The culture dish 15 of each position change location during the experiment, to maintain the mutually system of environment in a culture space 12
One, the cell character having the same in whole culture dish 15 reduces environmental error.
As an improvement specific implementation mode, rotating mechanism 0 include motor 01, shaft 02, plane bearing 03 and pass
Moving teeth 04, motor 01 are set to the bottom of babinet 11, and shaft 02 extends upward through the upper and lower of culture space 12 from motor 01
Wall penetrates multiple culture spaces 12, plane bearing 03 is arranged on the lower wall of each culture space 12, which is sheathed on
In shaft 02, driving cog 04 is set, which is sheathed in shaft 02 and links with shaft 02 on plane bearing 03；Culture
Pallet 14 is disk and there is a mounting groove 141, about 141 mounting groove to prolong through culture tray 14 and from 14 side of culture tray
Axle center is extended to, the center of the lower surface of culture tray 14 is provided with the kerve 142 coordinated with driving cog 04, kerve 142
Cell wall has the internal tooth for the transmission that is meshed with driving cog 04.
As shown in Figure 3,4, it is the embodiment of the first rotating mechanism 0 and corresponding culture tray 14.Motor 01 is arranged
In bottom, the shaft 02 on motor 01 penetrates 12 middle part of culture space being arranged above and below, the upper wall of shaft 02 and culture space 12
Place can be arranged sealing ring and upper down space is isolated, and avoid the pressure influence of different culture spaces 12 as possible；02 top of shaft passes through
One bearing is set to top, and the rotation of a shaft 02 enables 14 synchronous rotary of all culture trays, the unification of hold mode, culture
The lower wall setting plane bearing 03 and driving cog 04, driving cog 04 in space 12 are driven by shaft 02, plane bearing 03 and driving cog
04 supports culture tray 14 together.Culture tray 14 is put into from the case lid 13 that side is opened, the mounting groove 141 such as Fig. 4 of setting
It is shown, it enables shaft 02 enter mounting groove 141 on earth, 14 center of circle of culture tray is made to be overlapped with 02 center of circle of shaft, place culture tray
14 enable the internal tooth and 04 phase card of driving cog of kerve 142, can complete the setting of culture tray 14, stable transmission is not easy to shake.Training
Supporting ware 15 can circumferentially be evenly arranged in culture tray 14, enable all culture dishes 15 when rotation that can be in identical environment,
Ensure the accurate of experimental result.
As an improvement specific implementation mode, rotating mechanism 0 include motor 01, shaft 02 and plane bearing 03, electricity
Machine 01 is set to the bottom of babinet 11, shaft 02 extended upward through from motor 01 culture space 12 upper lower wall penetrate it is multiple
Culture space 12, setting plane bearing 03 is used to support culture tray 14 on the lower wall of each culture space 12, the plane bearing
03 is sheathed in shaft 02, and side channel 05 and jack 06 are provided in shaft 02；Culture tray 14 is disk and has a mounting groove
141, about 141 mounting groove extends to axle center, the slot bottom tool of mounting groove 141 through culture tray 14 and from 14 side of culture tray
There are inserting column 143, the mounting groove 141 of culture tray 14 to limit culture tray with installation, side channel 05 is coordinated at the side channel 05 of shaft 02
14, inserting column 143 is inserted into jack 06 when being installed in place.
As shown in Figure 5,6, it is the embodiment of second of rotating mechanism 0 and corresponding culture tray 14.Motor 01 is arranged
In bottom, the shaft 02 on motor 01 penetrates 12 middle part of culture space being arranged above and below, the upper wall of shaft 02 and culture space 12
Place can be arranged sealing ring and upper down space is isolated, and avoid the pressure influence of different culture spaces 12 as possible；02 top of shaft passes through
One bearing is set to top, and the rotation of a shaft 02 enables 14 synchronous rotary of all culture trays, the unification of hold mode, culture
Plane bearing 03 is arranged in the lower wall in space 12, and plane bearing 03 is used to support culture tray 14.Culture tray 14 is opened from side
Case lid 13 at be put into, the mounting groove 141 of setting is as shown in fig. 6, enable the side channel 05 of shaft 02 enter mounting groove 141, shaft 02
Section it is in the shape of an " I ", both sides are side channel 05, and mounting groove 141 is inserted between both sides side channel 05, lower wall limit training on side channel 05
The upper lower wall for supporting pallet 14, when shaft 02 reaches 141 bottom of mounting groove, the inserting column 143 of slot bottom is inserted into shaft 02
In jack 06, to enable culture tray 14 and shaft 02 coordinate linkage, the 03 good support culture tray of plane bearing of lower section
14, whole stable transmission is not easy to shake.Culture dish 15 can be circumferentially evenly arranged in culture tray 14, be owned when enabling rotation
Culture dish 15 can be in identical environment, ensure the accurate of experimental result.
As an improvement specific implementation mode, rotating mechanism 0 include motor 01, shaft 02, plane bearing 03 and drive
There is installation cavity 131, motor 01 to be set to 131 bottom of installation cavity for dynamic band 07, case lid 13, and shaft 02 upwardly extends from motor 01
It is arranged, case lid 13 is stretched out in the driving band 07 of setting linkage corresponding with 12 quantity of culture space, 07 part of driving band in shaft 02
Side wall；Setting plane bearing 03 is used to support culture tray 14 on the lower wall of each culture space 12, when babinet 11 covers case
When lid 13, the driving band 07 of stretching case lid 13 is in contact with the culture tray 14 in culture space 12 coordinates transmission.
It is the embodiment of the third rotating mechanism 0 and corresponding culture tray 14 as shown in Fig. 7,8,9.Culture space 12
Lower wall plane bearing 03 is set, plane bearing 03 is used to support culture tray 14.Motor 01 is set to the installation of case lid 13
131 bottom of chamber, the shaft 02 on motor 01 are set up, and 02 top of shaft is configured by a bearing, can be had in shaft 02
Another driving wheel is arranged on 13 wall of case lid for the setting driving wheel of body, and driving band 07, which is around on two driving wheels, to be run；Work as case
When lid 13 covers, the side of culture tray 14 can be butted on by stretching out the driving band 07 of the side wall of case lid 13, when motor 01 starts, be turned
Axis 02 drives the operation of driving band 07 to enable culture tray 14 rotate.The above structure is different from first two embodiment, and culture is empty
Between 12 and culture tray 14 setting and cooperation it is simpler, can more culture dishes 15 be set in culture tray 14, certainly
It is also circumferentially to be evenly arranged in culture tray 14, enables all culture dishes 15 when rotation that can be in identical environment, ensure
Experimental result it is accurate.
As an improvement specific implementation mode, the lower surface of culture tray 14 is provided with kerve 142, kerve 142 with
Plane bearing 03 matches.On the basis of the third embodiment, culture tray 14 is positioned over especially by the cooperation of kerve 142
On plane bearing 03, stable placement and transmission rotation are carried out.
As an improvement specific implementation mode, be provided with circumferentially evenly distributed several columns in culture tray 14
144, the holding tank 145 placed for culture dish 15 and the detent mechanism for being set to 145 outside of holding tank are provided on column 144
146, culture dish 15 is limited by detent mechanism 146 when culture dish 15 is placed into holding tank 145.Culture dish 15 especially by
Holding tank 145 on column 144 is placed, and is fixed by detent mechanism 146, and culture dish 15 is more steady when rotation
Fixed, culture tray 14 is also safer and convenient when picking and placeing, and is not in the case where culture dish 15 is fallen.Detent mechanism 146
Can be that the modes such as compression, clamping realize limit function.
As an improvement specific implementation mode, detent mechanism 146 include regulation ring 1461, pushing block 1462 and limited block
1463, there is regulation ring 1461 internal thread, 144 periphery of column to have external screw thread, and regulation ring 1461 is mobilizable to be threaded onto column 144
On, 144 periphery of column is axially provided with the first sliding rail 1441, which extends to 144 upper surface of column, and
144 upper surface of column is corresponding with 1441 position of the first sliding rail to be provided with the second radial sliding rail 1442, and pushing block 1462 passes through it
The first sliding block 1464 and the first sliding rail 1441 of upper setting, which coordinate, mobilizable is installed on the top of regulation ring 1461, limited block 1463
Coordinated by the second sliding block 1465 being arranged thereon and the second sliding rail 1442 and mobilizable is installed on 144 upper surface of column, pushing block
Being equipped with by angled-lift splits 1466 and 1467 slip of oblique sliding rail, sets on limited block 1463 between 1462 and limited block 1463
It is equipped with the rubber layer 1468 with the cooperation positioning of culture dish 15；When regulation ring 1461 rotate rise when, drive pushing block 1462 rise and
Limited block 1463 links inside sliding.
It is the concrete structure of detent mechanism 146 as shown in Figure 10,11, connection is realized by each interstructural ingenious cooperation
Dynamic and engaging function.Specifically when mounted, regulation ring 1461 is first inserted in column 144 from top, reaches the outer spiral shell of column 144
After at line, the screw-internal thread fit external screw thread of regulation ring 1461 screws in downwards, is positioned on column 144；Then by limited block 1463
The second sliding block 1465 from outside be packed into the second sliding rail 1442；Then the angled-lift splits 1466 of pushing block 1462 are packed into from upside and are limited
The oblique sliding rail 1467 of block 1463, the first sliding block 1464 with season pushing block 1462 is directed at the first sliding rail 1441 from top, in pushing block
Seasonal first sliding block 1464 of 1462 downward carry out activities enters the first sliding rail 1441, is placed in this way as pushing block 1462 slips down to bottom
In regulation ring 1461, limited block 1463 is also outer to slide into outermost position, and state is as shown in Figure 10.In use, experimenter exchanges
Section ring 1461 is rotated, and pushing block 1462 is raised movement, the corresponding slid inward of the meeting of limited block 1463, and rubber layer 1468 resists
Culture dish 15 in holding tank 145, which is realized, to be fixed, by the gravity of pushing block 1462 and regulation ring 1461, by each sliding rail and sliding block
Between existing frictional force, by the frictional force of rubber layer 1468 and culture dish 15, internal and external threads can by it is good occlusion and
Realize that positioning, state are as shown in figure 11.Locking to culture dish 15 can be released when reversely screwing off regulation ring 1461.The above positioning
The structure of mechanism 146 is simple, and convenient for manufacture processing, the good fixation for realizing culture dish 15 improves culture tray 14 and picks and places
Convenience and safety.In addition regulation ring 1461 is corresponding is arranged one, and pushing block 1462 and limited block 1463 can be in circles
Uniformly setting 2-4 groups are used on week.
As an improvement specific implementation mode, the one side or/and case lid 13 of babinet 11 are set as transparent material.It is training
In the case of supporting the rotation of pallet 14, the babinet 11 or case lid 13 of transparent material manufacture can enable experimenter intuitively be observed in side
To the concrete condition of each culture dish 15, convenient for being recorded during the experiment, to obtain corresponding data or progress
Debugging meets more experiment demands.
Above are merely preferred embodiments of the utility model, the scope of protection of the utility model is not limited merely to above-mentioned
Embodiment, technical solution belonging to the idea of the present invention belong to the scope of protection of the utility model.It should be pointed out that pair
For those skilled in the art, several improvements and modifications without departing from the principle of the utility model,
These improvements and modifications also should be regarded as the scope of protection of the utility model.
Claims (10)
1. a kind of small-sized cell culture unit of pressure controllable, it is characterised in that：Including incubator (1), adjustable air pump (2),
One adjustable valve (3), the second adjustable valve (4), pressure gauge (5) and third adjustable valve (6), the incubator (1) include babinet (11),
Multiple culture spaces (12) for being set in babinet (11) and the case lid (13) for being set to babinet (11) opening；It is described can regulating the qi flowing in the channels
Pump (2) connect after the first adjustable valve (3) by it is multiple enter pipeline (7) connected one to one respectively into multiple culture spaces (12)
In, each enter externally to connect bye-pass (9) on pipeline (7) and the second adjustable valve (4) is set on bye-pass (9)；Each training
Support space (12) external connection setting pressure gauge (5), each culture space (12) by it is multiple go out pipeline (8) external connection,
And it is multiple go out pipeline (8) collect and connect third adjustable valve (6).
2. a kind of small-sized cell culture unit of pressure controllable according to claim 1, it is characterised in that：It is described enter pipeline
(7) and go out pipeline (8) and be respectively arranged at the opposite two side of babinet (11), the pressure gauge (5) and go out pipeline (8) and set in homonymy
It sets.
3. a kind of small-sized cell culture unit of pressure controllable according to claim 2, it is characterised in that：Multiple trainings
It supports space (12) to be from top to bottom arranged, case lid (13) is opened in the side of babinet (11), is provided in the incubator (1)
Rotating mechanism (0), multiple culture spaces (12) are arranged dismountable culture tray (14), are put on the culture tray (14)
Several culture dishes (15) are set, the rotating mechanism (0) drives culture tray (14) to be rotated at work.
4. a kind of small-sized cell culture unit of pressure controllable according to claim 3, it is characterised in that：The whirler
Structure (0) includes motor (01), shaft (02), plane bearing (03) and driving cog (04), and the motor (01) is set to babinet
(11) bottom, the upper lower wall that the shaft (02) extends upward through culture space (12) at motor (01) penetrate multiple trainings
It supports space (12), setting plane bearing (03), the plane bearing (03) are sheathed on shaft on the lower wall of each culture space (12)
(02) on, driving cog (04) is set, which is sheathed in shaft (02) and and shaft on the plane bearing (03)
(02) it links；The culture tray (14) is disk and there is a mounting groove (141), the mounting groove (141) to run through training up and down
It supports pallet (14) and extends to axle center from culture tray (14) side, the centre bit of the lower surface of the culture tray (14) installs
It is equipped with the kerve (142) with driving cog (04) cooperation, the cell wall of the kerve (142) has the transmission that is meshed with driving cog (04)
Internal tooth.
5. a kind of small-sized cell culture unit of pressure controllable according to claim 3, it is characterised in that：The whirler
Structure (0) includes motor (01), shaft (02) and plane bearing (03), and the motor (01) is set to the bottom of babinet (11), institute
It states shaft (02) and extends upward through the upper lower wall of culture space (12) at motor (01) and penetrate multiple culture spaces (12), often
Plane bearing (03) is set on the lower wall of a culture space (12) and is used to support culture tray (14), which is arranged
In in shaft (02), side channel (05) and jack (06) are provided on the shaft (02)；The culture tray (14) be disk simultaneously
With a mounting groove (141), the mounting groove (141) is run through culture tray (14) and is extended from culture tray (14) side up and down
To axle center, the slot bottom of the mounting groove (141) has inserting column (143), the mounting groove (141) and shaft of the culture tray (14)
(02) installation, side channel (05) is coordinated to limit culture tray (14) at side channel (05), inserting column (143) is inserted into jack when being installed in place
(06)。
6. a kind of small-sized cell culture unit of pressure controllable according to claim 3, it is characterised in that：The whirler
Structure (0) includes motor (01), shaft (02), plane bearing (03) and driving band (07), and the case lid (13) has installation cavity
(131), the motor (01) is set to installation cavity (131) bottom, and the shaft (02) upwardly extends setting at motor (01),
The driving band (07) of setting linkage corresponding with culture space (12) quantity on the shaft (02), the driving band (07) part
Stretch out the side wall of case lid (13)；Setting plane bearing (03) is used to support culture tray on the lower wall of each culture space (12)
(14), the driving band (07) for stretching out case lid (13) when babinet (11) covers case lid (13) and the culture in culture space (12)
Pallet (14), which is in contact, coordinates transmission.
7. a kind of small-sized cell culture unit of pressure controllable according to claim 6, it is characterised in that：The culture support
The lower surface of disk (14) is provided with kerve (142), and the kerve (142) matches with plane bearing (03).
8. a kind of small-sized cell culture unit of pressure controllable according to claim 3 or 4 or 5 or 6 or 7, feature exist
In：It is provided with circumferentially evenly distributed several columns (144) on the culture tray (14), is arranged on the column (144)
There are the holding tank (145) placed for culture dish (15) and the detent mechanism (146) being set on the outside of holding tank (145), works as training
It supports and is limited culture dish (15) by detent mechanism (146) when ware (15) is placed into holding tank (145).
9. a kind of small-sized cell culture unit of pressure controllable according to claim 8, it is characterised in that：The localization machine
Structure (146) includes regulation ring (1461), pushing block (1462) and limited block (1463), and the regulation ring (1461) has internal thread,
Column (144) periphery has external screw thread, and the regulation ring (1461) is mobilizable to be threaded onto on column (144), the column
(144) periphery is axially provided with the first sliding rail (1441), which extends to column (144) upper surface,
And column (144) upper surface is corresponding with the first sliding rail (1441) position is provided with radial the second sliding rail (1442), it is described to push away
Block (1462) is installed on regulation ring by the way that the first sliding block (1464) and the first sliding rail (1441) cooperation being arranged thereon are mobilizable
(1461) top, the limited block (1463) can with the second sliding rail (1442) cooperation by the second sliding block (1465) being arranged thereon
It is movable to be installed on column (144) upper surface, pass through angled-lift splits (1466) between the pushing block (1462) and limited block (1463)
With being equipped with for oblique sliding rail (1467) slip, it is provided on the limited block (1463) and culture dish (15) cooperation positioning
Rubber layer (1468)；When regulation ring (1461) rotate rise when, drive pushing block (1462) rise and limited block (1463) link to
Interior sliding.
10. a kind of small-sized cell culture unit of pressure controllable according to claim 3 or 4 or 5 or 6 or 7, feature exist
In：The one side or/and case lid (13) of the babinet (11) are set as transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820247585.0U CN208038466U (en) | 2018-02-11 | 2018-02-11 | A kind of small-sized cell culture unit of pressure controllable |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820247585.0U CN208038466U (en) | 2018-02-11 | 2018-02-11 | A kind of small-sized cell culture unit of pressure controllable |
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| CN208038466U true CN208038466U (en) | 2018-11-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804549A (en) * | 2019-11-20 | 2020-02-18 | 山东中医药大学附属医院 | Low-pressure and low-oxygen cell experimental device |
-
2018
- 2018-02-11 CN CN201820247585.0U patent/CN208038466U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110804549A (en) * | 2019-11-20 | 2020-02-18 | 山东中医药大学附属医院 | Low-pressure and low-oxygen cell experimental device |
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