CN208026751U - A kind of glycosylated albumin detection kit - Google Patents
A kind of glycosylated albumin detection kit Download PDFInfo
- Publication number
- CN208026751U CN208026751U CN201820551801.0U CN201820551801U CN208026751U CN 208026751 U CN208026751 U CN 208026751U CN 201820551801 U CN201820551801 U CN 201820551801U CN 208026751 U CN208026751 U CN 208026751U
- Authority
- CN
- China
- Prior art keywords
- bottle
- reagent
- bottom wall
- detection kit
- glycosylated albumin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a kind of glycosylated albumin detection kit, which includes box body and be covered on the box cover of box body, further includes fixture and three reagent bottles.Wherein, fixture is set in box body, and fixture is set there are three storage tank；Correspondence is positioned over three storage tanks and is adapted to storage tank three reagent bottles respectively；Reagent bottle includes bottle cap and bottle, and the inner surface of the bottom wall of bottle is in inclined-plane, and bottle cap corresponds to the lower setting of bottom wall inner surface.Technical solutions of the utility model improve the structure at reagent bottle bottom, improve the accuracy of testing result.
Description
Technical field
The utility model is related to detection device technology field, more particularly to a kind of glycosylated albumin detection kit.
Background technology
Diabetes are a kind of common endocrine metabolism diseases, can cause the lesions such as retina, kidney, nerve and it is various simultaneously
Disease is sent out, it is the effective means treated diabetes and prevent its various complication strictly to control blood glucose level.Glycosylated albumin
(GA) it can be employed as the index of glycemic control with glycosylated hemoglobin (HbA1c), glycated protein (GSP).
GA is the early stage glycation product that glucose in blood occurs that nonenzymatic glycosylation is reacted with albumin.Because albumin is in body
Interior half-life period is shorter (about 17d), and the combination speed of albumin and blood glucose is faster than hemoglobin, so GA is to blood glucose in a short time
Variation is than HbA1c sensitivities, it is generally recognized that GA measures the glycemic control situation that can reflect patient recent (about 2 weeks).GA has become at present
Clinically to apply wide blood sugar monitoring index.
The method of earliest detection GA is high performance liquid chromatography, has the characteristics that accuracy is high, but due to detect needed for
Cost is higher, and the sample size of single treatment is small, so that being failed to answer extensively by larger limitation in clinical quick detection
With.Ketoamine oxidase method detection GA (LucicaGA-L) can realize fast reaction, and have with enzyme process Fast Detection Technique
There is detection result highly sensitive, that specificity is good.Reagent bottle in current used kit, bottom of bottle is plane, needs to try
The inclination of agent bottle could use reagent completely.However be easy to shake reagent generation bubble in this way, to influence testing result.
Utility model content
The main purpose of the utility model is to provide a kind of glycosylated albumin detection kit, it is intended to improve reagent bottle bottom
Structure improves the accuracy of testing result.
To achieve the above object, the utility model proposes glycosylated albumin detection kit, including box body and be covered on
The box cover of the box body further includes：Fixture is set in the box body, and the fixture is set there are three storage tank；Three reagents
Bottle, correspondence is positioned over three storage tanks and is adapted to the storage tank three reagent bottles respectively；The reagent bottle packet
Bottle cap and bottle are included, the bottle has side wall and bottom wall, and the inner surface of the bottom wall is in inclined-plane, and the bottle cap corresponds to the bottom
The lower of wall inner surface is arranged.
Optionally, there are the bottle opposite both ends, the both ends of the bottle bottom wall to be equipped with support leg.
Optionally, the sectional area of the bottle gradually successively decreases from one end of the bottle to the other end.
Optionally, the inner surface of the bottle bottom wall is from the relatively small one end of the bottle sectional area to the bottle section
The relatively large one end of product tilts.
Optionally, in the relatively small one end of the bottle sectional area, the support leg from the outer end of the bottle bottom wall to
Interior extension.
Optionally, the bottle is relatively set with recessing groove, and the recessing groove is upwardly extended from the bottom end of the side wall
It is formed.
Optionally, the reagent bottle is in dark-brown.
Optionally, the fixture is cystosepiment.
Technical solutions of the utility model are in the reagent bottle on inclined-plane by using bottom wall inner surface, and bottle cap is made to correspond in bottom wall
The lower on surface is arranged, in use, reagent is flowed downwards along inner surface, and by direct sample at bottle cap, no
It can only make reagent using completely, avoid reagent waste.Meanwhile prevent in order to by reagent using completely to the shaking of reagent,
The generation for avoiding bubble effectively improves the accuracy of testing result to reduce error.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, the structure that can also be shown according to these attached drawings obtains other attached drawings.
Fig. 1 is a kind of structural schematic diagram of one embodiment of glycosylated albumin detection kit of the utility model；
Fig. 2 is the positive structure schematic of reagent bottle in Fig. 1；
Fig. 3 is the upward view of reagent bottle in Fig. 1；
Drawing reference numeral explanation：
| Label | Title | Label | Title |
| 1 | Glycosylated albumin detection kit | 10 | Box cover |
| 20 | Box body | 30 | Reagent bottle |
| 31 | Bottle cap | 32 | Bottle |
| 33 | Support leg | 34 | Recessing groove |
| 40 | Fixture |
The embodiments will be further described with reference to the accompanying drawings for the realization, functional characteristics and advantage of the utility model aim.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describing, it is clear that described embodiment is only a part of the embodiment of the utility model, rather than all
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative work premise
Lower obtained every other embodiment, shall fall within the protection scope of the present invention.
It is to be appreciated that the directional instruction (such as up, down, left, right, before and after ...) of institute in the utility model embodiment
It is only used for explaining relative position relation, the motion conditions etc. under a certain particular pose (as shown in the picture) between each component, such as
When the fruit particular pose changes, then directionality instruction also correspondingly changes correspondingly.
In addition, the description for being related to " first ", " second " etc. in the present invention is used for description purposes only, and cannot understand
To indicate or implying its relative importance or implicitly indicate the quantity of indicated technical characteristic.Define as a result, " first ",
The feature of " second " can explicitly or implicitly include at least one of the features.In addition, the technical side between each embodiment
Case can be combined with each other, but must can be implemented as basis with those of ordinary skill in the art, when the combination of technical solution
Conflicting or cannot achieve when occur will be understood that the combination of this technical solution is not present, also not in the requires of the utility model
Protection domain within.
The utility model proposes a kind of glycosylated albumin detection kits.
Referring to figs. 1 to 3.
In the utility model embodiment, the glycosylated albumin detection kit 1 is including box body 20 and is covered on box body 20
On box cover 10, further include fixture 40 and three reagent bottles 30.Wherein, fixture 40 is set in box body 20, and fixture 40 is set
There are three storage tanks；Correspondence is positioned over three storage tanks and is adapted to storage tank three reagent bottles 30 respectively；Reagent bottle 30 includes
Bottle cap 31 and bottle 32, bottle 32 have side wall and bottom wall, and the inner surface of bottom wall is in inclined-plane, and bottle cap 31 corresponds to bottom wall inner surface
Lower is arranged.
Specifically, which includes mainly two parts, and a part is packaging, i.e. box body 20 and lid
Box cover 10 on box body 20；Another part is the reagent used needed for detection, i.e. reagent bottle 30.It is aoxidized with ketoamine
Enzyme process is detected glycosylated albumin, and it includes specific proteases, Ketoamine oxidase and peroxidase to need reagent to be used,
Therefore the kit is set there are three reagent bottle 30.In order to ensure placement of the reagent bottle 30 in kit, avoid colliding or
It tilts, fixture 40 is provided in box body 20, fixture 40 is made to be equipped with storage tank, and the shape of storage tank and reagent bottle 30
Adaptation, to ensure the steady placement of reagent bottle 30.
Reagent bottle 30 includes bottle cap 31 and bottle 32.Under normal conditions, the inner surface of 30 bottom wall of reagent bottle is plane, in this way
Design need to make bottle 32 to tilt in use so that reagent is flowed to same direction, so as to use reagent
Completely, it avoids wasting.However so operation is easy to cause to shake to reagent, makes to generate bubble in reagent, is generated to testing result
It influences so that the error of detected value increases.Therefore, the inner surface of 32 bottom wall of bottle is set as inclined-plane, with difference in height, made
Used time, reagent can flow to the lower of bottle 32 naturally along bottom wall inner surface without tilting and rocking.Meanwhile bottle cap 31 being made to correspond to
The lower of bottom wall is arranged, and in the lower direct sample of 32 bottom wall of bottle, not only avoids wasting, and prevent the generation of bubble, have
Effect improves the accuracy of testing result.
In addition, can also be by the way that support construction be arranged in the bottom of bottle 32, to increase between 32 bottom wall of bottle and placed side
Contact area so that bottle 32 is placed steadily.For example, the outer surface of bottom wall is made to be plane, shape between outer surface and inner surface
At hollow, bottle 32 can steadily be placed by outer surface.Or support leg 33 is set in the bottom wall of bottle 32, pass through support leg 33
The placing height for adjusting bottom wall is allowed to be in identical height, steady to place.
In practical applications, the amount of capacity of reagent bottle 30 is customized according to specific needs.Three reagent bottles 30 are solid
Arrangement on earnest 40 can be laid out in parallel, can also be staggered, so that compact-sized, while disclosure satisfy that production makes
Standard and requirement.
Technical solutions of the utility model are in the reagent bottle 30 on inclined-plane by using bottom wall inner surface, and bottle cap 31 is made to correspond to bottom
The lower of wall inner surface is arranged, in use, reagent is flowed downwards along inner surface, and by directly being taken at bottle cap 31
Sample can not only make reagent using completely, avoid reagent waste.Meanwhile it preventing to use reagent completely to reagent
It shakes, avoids the generation of bubble, to reduce error, effectively improve the accuracy of testing result.
In the present embodiment, as shown, making bottle 32 that there are opposite both ends, the both ends of 32 bottom wall of bottle to be equipped with support leg
33.By support leg 33, on the other hand the contact area on the one hand increasing when reagent bottle 30 is placed and placed side adjusts bottle
32 balance is allowed to be in the same horizontal position, realizes steady place.
Further, the sectional area of bottle 32 is made gradually to successively decrease from one end of bottle 32 to the other end.Make the body of bottle 32
Product is gradually reduced from one end to the other side, and the inner wall of bottle 32 can form the effect of the precompressed to reagent, and reagent is faster from bottom wall
The eminence of inner surface flows downwards.On this basis, the inner surface of 32 bottom wall of bottle from 32 sectional area of bottle it is relatively small one
It holds to the relatively large one end of 32 sectional area of bottle and tilts.So that flow to space big and low for reagent small one end in space out of bottle 32
The other end at place.This is because the bottom wall lower of bottle 32 has big space, reagent is can avoid because flowing velocity is more than sampling
When speed, reagent is flowed out from bottleneck, is caused to waste and be polluted.Also, make the top bottle mouth position of bottle 32 that there is big area, just
In the assembly of bottle cap 31 and bottle 32.
In addition, in the relatively small one end of 32 sectional area of bottle, support leg 33 extends internally from the outer end of 32 bottom wall of bottle.With
The area for increasing the support leg 33 of bottle 32 small one end of bottom wall sectional area, make reagent bottle 30 by the placement of support leg 33 more
Surely.
As shown, bottle 32 is relatively set with recessing groove 34, recessing groove 34 upwardly extends to be formed from the bottom end of side wall.With
The side wall of bottle 32 is set to be recessed inwardly to form screens.In test, tester is consolidated reagent bottle 30 by the recessing groove 34
It is fixed, easy to operation and detection.Further, since test reagent is light sensitive, in order to avoid the reagent in reagent bottle 30 is shown in
Photodegradation makes reagent bottle 30 provide the stability of reagent in dark-brown to meet the preservation requirement of reagent.
As shown, fixture 40 is cystosepiment.Reagent bottle 30 is fixed by cystosepiment, prevents reagent bottle 30 because of collision
Caused by damage.It of courses, except foam-expelling plate, foam-rubber cushion also can be used in fixture 40.
In addition, in the present embodiment, three reagent bottles 30 are respectively provided with R1 reagents, R2 reagents, R3 reagents, wherein R1
Include Proteinase K in reagent, content is 9500~10500U/L；Include Ketoamine oxidase in R2 reagents, content is 25~
30U/ml；Peroxidase, content are 90~95U/ml；Include bromocresol green in R3 reagents, content is 1.8 × 10-4mol/
L.Specifically, R1 reagents include：Three-hydroxymethyl aminomethane buffer solutions (Tris-HCl), a concentration of 50mmol/L；Protease
K, content 10000U/L；CaCl2, a concentration of 3mmol/L；4-AA, content 2.5mmol.R2 is tried
Agent includes：Tris-HCl, a concentration of 15mmol/L；- 3 meta-aminotoluene propanesulfonate (Toos) of 2- hydroxyls, it is a concentration of
10mmol/L；Ketoamine oxidase, content 28U/ml；Peroxidase, content 92U/ml.R3 reagents include：Amber
Acid buffer, a concentration of 0.05mol/L；Bromocresol green, a concentration of 1.8 × 10-4mol/L.In test, make reagent R1 and sample
This is reacted, and the glycosylated albumin in sample is catalytically decomposed with Proteinase K, generates glycated amino acid.Reagent 2 is added,
Glycated amino acid is set to be catalytically decomposed under the action of Ketoamine oxidase, generation arabino-hexosone, amino acid and hydrogen peroxide, and
Under the action of peroxidase, hydrogen peroxide is replaced with 4- amino to react than woods and Toos generations Trinder, generates bluish violet production
Object.Then coloration occurs with bromocresol green in reagent 3, by measuring absorbance, you can obtain the white egg of saccharification in sample
White concentration.
The above is only the preferred embodiment of the present invention, and it does not limit the scope of the patent of the present invention,
It is every under the inventive concept of the utility model, equivalent structure made based on the specification and figures of the utility model becomes
It changes, or directly/be used in other related technical areas indirectly and be included in the scope of patent protection of the utility model.
Claims (8)
1. a kind of glycosylated albumin detection kit, including box body and the box cover for being covered on the box body, which is characterized in that also wrap
It includes：
Fixture is set in the box body, and the fixture is set there are three storage tank；
Three reagent bottles, correspondence is positioned over three storage tanks and is adapted to the storage tank three reagent bottles respectively；
The reagent bottle includes bottle cap and bottle, and the bottle has side wall and bottom wall, and the inner surface of the bottom wall is in inclined-plane, the bottle
The lower setting of the corresponding bottom wall inner surface of lid.
2. glycosylated albumin detection kit as described in claim 1, which is characterized in that the bottle has opposite two
The both ends at end, the bottle bottom wall are equipped with support leg.
3. glycosylated albumin detection kit as claimed in claim 2, which is characterized in that the sectional area of the bottle is described in
Gradually successively decrease to the other end one end of bottle.
4. glycosylated albumin detection kit as claimed in claim 3, which is characterized in that the inner surface of the bottle bottom wall is certainly
The relatively small one end of the bottle sectional area is tilted to the relatively large one end of the bottle sectional area.
5. glycosylated albumin detection kit as claimed in claim 3, which is characterized in that relatively small in the bottle sectional area
One end, the support leg extends internally from the outer end of the bottle bottom wall.
6. glycosylated albumin detection kit as described in claim 1, which is characterized in that the bottle is relatively set with resigning
Slot, the recessing groove upwardly extend to be formed from the bottom end of the side wall.
7. glycosylated albumin detection kit as described in claim 1, which is characterized in that the reagent bottle is in dark-brown.
8. glycosylated albumin detection kit as described in claim 1, which is characterized in that the fixture is cystosepiment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820551801.0U CN208026751U (en) | 2018-04-17 | 2018-04-17 | A kind of glycosylated albumin detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820551801.0U CN208026751U (en) | 2018-04-17 | 2018-04-17 | A kind of glycosylated albumin detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208026751U true CN208026751U (en) | 2018-10-30 |
Family
ID=63910767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820551801.0U Active CN208026751U (en) | 2018-04-17 | 2018-04-17 | A kind of glycosylated albumin detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208026751U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110687306A (en) * | 2019-10-30 | 2020-01-14 | 深圳上泰生物工程有限公司 | Direct-connection on-board hemolytic enzyme method double-reagent glycosylated hemoglobin detection kit |
-
2018
- 2018-04-17 CN CN201820551801.0U patent/CN208026751U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110687306A (en) * | 2019-10-30 | 2020-01-14 | 深圳上泰生物工程有限公司 | Direct-connection on-board hemolytic enzyme method double-reagent glycosylated hemoglobin detection kit |
| CN110687306B (en) * | 2019-10-30 | 2023-04-25 | 深圳上泰生物工程有限公司 | Double-reagent glycosylated hemoglobin detection kit for direct on-machine hemolysis enzyme method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8721853B2 (en) | Fructosyl peptidyl oxidase | |
| US20030068666A1 (en) | Dual glucose-hydroxybutyrate analytical sensors | |
| CN208026751U (en) | A kind of glycosylated albumin detection kit | |
| CN101484809A (en) | Methods for assaying percentage of glycated hemoglobin | |
| EP1726955A1 (en) | Method of stabilizing oxidizable color-assuming reagent | |
| CN109239059A (en) | A kind of glycated serum protein assay kit and its preparation method and application | |
| CN107870170B (en) | A kind of kit of luminol chemiluminescence analysis measurement glycated albumin | |
| WO2007072941A1 (en) | Method for determination of glycosylated protein | |
| JP2013074877A (en) | Multilayer test piece for assaying glycosylated hemoglobin and assay method using the same | |
| KR20210014754A (en) | Blood sample assay method | |
| CN110333356A (en) | A kind of compound Quality Control object of urine special proteins and preparation method | |
| CN1489693B (en) | Method and devices for quantitation of glycated protein | |
| Toyota et al. | Determination of total protein in serum using a tyrosinase enzyme electrode | |
| CN109946352A (en) | Reaction reagent and its application for potential type biosensor | |
| CN105784616A (en) | Method for detecting cysteine or acetylcysteine on basis of bimetal nanometer clusters | |
| CA2658023A1 (en) | Biosensor | |
| CN102384971A (en) | Spittle alcoholicity test strip and production method thereof | |
| Kano | Redox potentials of proteins and other compounds of bioelectrochemical interest in aqueous solutions | |
| CN109212238A (en) | A kind of glycosylated albumin assay kit | |
| US20110207160A1 (en) | Method for measuring low density lipoprotein cholesterol | |
| CN211318294U (en) | Electrochemical biosensor capable of rapidly detecting | |
| CN202351241U (en) | Saliva alcohol content testing strip | |
| WO2017096373A1 (en) | Systems and methods for interference correction from hemoglobin variants | |
| KR101593114B1 (en) | Biosensor | |
| WO2023219148A1 (en) | Fluid device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |