CN207933460U - A kind of T cell CAR developed by molecule analysis detection kit - Google Patents
A kind of T cell CAR developed by molecule analysis detection kit Download PDFInfo
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- CN207933460U CN207933460U CN201820111078.4U CN201820111078U CN207933460U CN 207933460 U CN207933460 U CN 207933460U CN 201820111078 U CN201820111078 U CN 201820111078U CN 207933460 U CN207933460 U CN 207933460U
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Abstract
The utility model discloses a kind of T cell CAR developed by molecule to analyze detection kit,The device includes box body,The box body is made of damp-proof layer and insulating layer,The insulating layer is located on the inside of damp-proof layer,The top side of the box body is hinged with box cover by hinge,The box cover inside top is equipped with soft rubber cushion,The intracavity bottom of the box body is equipped with shockproof decompression layer,The top of the shockproof decompression layer is equipped with built-in box,The built-in box both sides are connected with box body both sides inner wall respectively by spring,The built-in box is fixed plate interval and forms several chambers,The top of each chamber is equipped with cork,Each chamber is internally provided with protective layer,The protective layer is equipped with groove,Reagent bottle is equipped in the groove,The reagent bottle is followed successively by buffering agents bottle,APC CD3 reagent bottles,Biotin Protein L reagent bottles and SA PE reagent bottles.The detection kit is reasonable for structure, water, shock, and heat insulation effect is good, and great convenience is brought to user's use.
Description
Technical field
The utility model is related to technical field of molecular biology, and in particular to a kind of T cell CAR developed by molecule analysis detection
Kit.
Background technology
In recent years, growing with tumor incidence, tumour immunotherapy has obtained more and more concerns and has answered
With the especially appearance of CAR-T technologies makes the mankind obtain landmark development to the control of tumour, it is not only to one
A little solid tumor such as lymthomas, neuroblastoma have good therapeutic effect, or even in acute or chronic lymphocytic
It can work miracle in blood disease.CAR-T (Chimeric Antigen Receptor T-Cell Immunotherapy, it is embedding
Close antigen receptor T cell immunotherapy) it is to obtain to carry identification specific for tumour antigen receptor T lymphocytes by genetic modification
Personalized treatment method in recent years, splendid treatment effect has been shown in multiple clinical tests for CAR-T therapies
Fruit, right and wrong are often with potential treatment of cancer new way.Wherein, CAR genes can express, the height of expression efficiency, CAR-T it is thin
The activity and purity of born of the same parents can all directly affect the elimination efficiency of CAR-T cells on cancer cells, therefore usually need in the prior art
Analysis detection is carried out to the expression of T cell CAR molecules, generally requires to use a variety of reagents in detection process, needs multiple examinations
Agent container dispenses, to carrying and storage brings great inconvenience, and most of the cell origin of CAR-T cell clinical tests
It is all taken from the periphery blood T cell of patient itself, blood process is taken extremely to be not easy.There are a variety of kits on the market at present, so
And performance is relatively simple, functionalized use is not apparent enough, simple in structure, it is difficult to ensure to carry the safety for shaking reagent bottle on the way.
Utility model content
The purpose of this utility model is to provide a kind of T cell CAR developed by molecule to analyze detection kit, has effective
The advantages that detection T cell CAR molecules are with avoiding reagent bottle from colliding on the way because of carrying or shake rupture, and there is preferably moisture-proof guarantor
Temp effect, and it is easy to carry.
To achieve the above objectives, the technical scheme adopted by the utility model is that：
Box body, the box body are made of damp-proof layer and insulating layer, and the insulating layer is located on the inside of damp-proof layer, the box body
Top side is hinged with box cover by hinge, and the box cover inside top is equipped with soft rubber cushion, and the intracavity bottom of the box body is equipped with
Shockproof decompression layer, the top of the shockproof decompression layer are equipped with built-in box, the built-in box both sides by spring respectively with box body two
Side inner wall is connected, and the built-in box is fixed plate interval and forms several chambers, and the top of each chamber is equipped with cork, each
The chamber is internally provided with protective layer, and the protective layer is equipped with multiple grooves, and being equipped with buffer solution in each groove tries
One kind in agent bottle, APC-CD3 reagent bottles, Biotin-Protein L reagent bottles and SA-PE reagent bottles.
Based on the above technical solution, the quantity of the groove is at least four.
Based on the above technical solution, the box cover outside top is equipped with handle, and inside is surrounded by seal washer.
Based on the above technical solution, the box body is equipped with coded lock.
Based on the above technical solution, it is equipped with thermometer at the top of the inboard wall of cartridge side and box cover inner wall.
Based on the above technical solution, all reagent bottles include body and bottle cap, are led between body and bottle cap
Cross threaded connection.
Based on the above technical solution, further include at least one in multiple grooves for accommodating the spare of spare reagent bottle
Groove.
Based on the above technical solution, the buffering agents bottle, APC-CD3 reagent bottles, Biotin-Protein
The tag slot for identifying title is equipped on L reagent bottles and SA-PE reagent bottles.
Based on the above technical solution, all reagent bottles are brown reagent bottle.
Compared with prior art, the utility model has the advantage of：
The detection kit is reasonable for structure, multiple functional, by the way that damping spring, soft rubber cushion, protective layer are arranged in box body
With shockproof decompression layer, reagent bottle can be effectively prevented from and collide when carrying and rupture, while reducing reagent bottle itself
Shaking, or even when reagent babinet surprisingly falls on the ground upper, also can be well protected the safety of reagent bottle, will not be hit because of violent
It hits and is crushed.In addition, the design of moisture-proof insulating layer ensures the stabilization of tray interior environment, it is ensured that reagent will not in certain time
Go bad because of environmental change, in the way transported and stored, great convenience is brought to user's use.
Description of the drawings
Fig. 1 is the structural schematic diagram in the utility model embodiment.
In figure：1- box bodys, 10- damp-proof layers, 11- insulating layers, 2- box covers, 20- soft rubber cushions, 21- handles, the shockproof decompressions of 3-
Layer, box built in 4-, 40- fixed plates, 41- chambers, 42- corks, 43- protective layers, 44- buffering agents bottles, 45-APC-CD3 examinations
Agent bottle, 46-Biotin-Protein L reagent bottles, 47-SA-PE reagent bottles, 5- springs, 6- thermometers.
Specific implementation mode
The embodiments of the present invention are described in further detail below in conjunction with attached drawing.
Embodiment one
Shown in Figure 1, the utility model embodiment provides a kind of T cell CAR developed by molecule analysis detection kit, packet
Box body 1 is included, box body 1 is made of damp-proof layer 10 and insulating layer 11, and wherein insulating layer 11 is located at 10 inside of damp-proof layer, and this structure is set
Count the stabilization for 1 internal temperature of invasion and box body for making box body 1 that can prevent extraneous aqueous vapor on to greatest extent.Box
Body 1 can be any appropriate shape, the convenience used for user and daily use habit, and the shape of box body 1 is herein
Preferably cuboid.The top side of box body 1 is hinged with box cover 2 corresponding with 1 opening shape of box body by hinge, in box cover 2
Side roof part is equipped with soft rubber cushion 20, and soft rubber cushion 20 has certain thickness, during transport or carrying, can play shock buffering
Effect.The outside top of box cover 2 is equipped with handle 21, and inside is surrounded by seal washer, can shape after box cover 2 is fastened with box body 1
At preferable sealed environment.Due to may relate to maintain secrecy for the reagent of detection, box cover 2 is connect by coded lock with box body
It fastens, in use, corresponding password can be set according to actual needs.The intracavity bottom of box body 1 is additionally provided with anti-
Shake decompression layer 3, shockproof decompression layer 3 can be any suitable material with shockproof decompression, and foamed material it is light, it is firm,
It is shock-absorbing, the low moisture absorption, easily molded and the features such as possess good water resistance, thermal insulation, low price, be widely used in packaging,
The fields such as heat preservation, waterproof, heat-insulated, damping are one of most widely used plastics in the world today, can be applied to electric appliance, instrument
The packaging etc. of instrument, the Shockproof packaging of craftwork and other rapid wear valuables and fast food, shockproof decompression layer 3 exist
This is preferably PVC foamed materials.In addition, being equipped with thermometer 6 at the top of 1 inner wall side of box body and 2 inner wall of box cover, facilitate user
Check box body temperature.
Shown in Figure 1, the top of shockproof decompression layer 3 is equipped with built-in box 4, and built-in box 4 can be any suitable material,
Due to PVC antiseptic properties are good, heat-insulated, waterproof, resistance to compression bending resistance, it is cheap, built-in box 4 herein be preferably PVC material.Built-in box
4 both sides are connected with 1 both sides inner wall of box body respectively by spring 5, when transport or carry shake on the way when, spring 5 can be with
It the direction that built-in box 4 shakes and gives cushion effect, mitigate the shaking of reagent bottle.Built-in box 4 be fixed plate 40 interval be divided into it is several
The inside of a chamber 41, each chamber 41 is equipped with protective layer 43, and protective layer 43 is preferably herein PVC foamed materials, and is had
Certain thickness, has the function of bumper and absorbing shock.Protective layer 43 is equipped with groove, and is equipped with accordingly in corresponding groove
Buffering agents bottle 44, APC-CD3 reagent bottles 45, Biotin-Protein L reagent bottles 46 and SA-PE reagent bottles 47, it is each recessed
Slot places a reagent bottle, is separated by fixed plate 40 and protective layer 43 between reagent bottle, when needing to take reagent, by reagent
Bottle takes out from groove.Since the reagent for detecting used is more, buffering agents bottle 44, APC-CD3 reagent bottles 45, Biotin-
It is equipped with the tag slot for identifying title on Protein L reagent bottles 46 and SA-PE reagent bottles 47, prevents user from obscuring and causes
Detection failure.In order to further facilitate the detection of user, it is more with standby user placement that protective layer 43 is equipped with two spare grooves
Remaining reagent bottle.All reagent bottles are brown, reagent can be made to be kept in dark place, and reagent bottle includes body and bottle cap, body and bottle
It is connected through a screw thread between lid, can guarantee that reagent will not be spilt inside bottle, it is ensured that reagent bottle has good sealing performance.
The top of each chamber 41 is equipped with cork 42, and what cork 42 can be tighter is tucked into the opening of each cavity 41, and cork 42
Bottom is just in contact with the bottle cap of reagent bottle, and centre does not have redundant space, prevents reagent bottle from being shaken in cavity, can prevent yet
In the case where box cover 2 is opened, reagent bottle is fallen from groove.It fills in and extracts on cavity in order to facilitate cork 42, it is preferred that
The top of cork 42 is more slightly higher than the top of cavity.
Operation principle when detection is as follows：T cell to be detected is washed with one milliliter of Buffer first, carries out three
Centrifugally operated per minute is enclosed in the 1000 of minute, and three hectolambda buffer are added and are resuspended, and are added to different measuring samples
Five microlitres of Biotin-Protein L are placed 40 minutes at a temperature of four degrees Celsius, then carry out three minutes 1,000 circles often
The centrifugally operated of minute is used in combination one milliliter of Buffer to carry out carrying out washing treatment, then different measuring samples is added with seven microlitres of SA-
PE and four microlitre of CD3-APC carries out being protected from light processing, places at a temperature of four degrees Celsius 40 minutes, carries out three minutes again
1,000 circle centrifugally operateds per minute, be used in combination one milliliter of Buffer to carry out carrying out washing treatment, three hectolambda buffer is added and weigh
It hangs, proceeds by flow cytometer detection.
The utility model is not only limited to above-mentioned preferred forms, anyone can obtain under the enlightenment of the utility model
Go out other various forms of products, however, make any variation in its shape or structure, it is every to have and the utility model phase
Same or close technical solution, within its protection domain.
Claims (9)
1. a kind of T cell CAR developed by molecule analyzes detection kit, which is characterized in that it includes：Box body (1), the box body
(1) it is made of damp-proof layer (10) and insulating layer (11), the insulating layer (11) is located on the inside of damp-proof layer (10), the box body (1)
Top side box cover (2) is hinged with by hinge, box cover (2) inside top is equipped with soft rubber cushion (20), the box body (1)
Intracavity bottom be equipped with shockproof decompression layer (3), it is described it is shockproof decompression layer (3) top be equipped with built-in box (4), it is described built in box
(4) both sides are connected with box body (1) both sides inner wall respectively by spring (5), and the built-in box (4) is fixed plate (40) interval and is formed
The top of several chambers (41), each chamber (41) is equipped with cork (42), and being internally provided with for each chamber (41) is anti-
Sheath (43), the protective layer (43) are equipped with multiple grooves, buffering agents bottle (44), APC- are equipped in each groove
One kind in CD3 reagent bottles (45), Biotin-Protein L reagent bottles (46) and SA-PE reagent bottles (47).
2. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：The groove
Quantity be at least four.
3. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：The box cover
(2) outside top is equipped with handle (21), and inside is surrounded by seal washer.
4. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：The box body
(1) it is equipped with coded lock.
5. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：The box body
(1) it is equipped with thermometer (6) at the top of inner wall side and box cover (2) inner wall.
6. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：It is all described
Reagent bottle includes body and bottle cap, is connected through a screw thread between body and bottle cap.
7. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：Multiple grooves
In further include at least one for accommodating the spare groove of spare reagent bottle.
8. a kind of T cell CAR developed by molecule as described in claim 1 analyzes detection kit, it is characterised in that：The buffering
On liquid reagent bottle (44), APC-CD3 reagent bottles (45), Biotin-Protein L reagent bottles (46) and SA-PE reagent bottles (47)
It is equipped with the tag slot for identifying title.
9. analyzing detection kit according to a kind of any T cell CAR developed by molecule of claim 1-8, it is characterised in that：
All reagent bottles are brown reagent bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820111078.4U CN207933460U (en) | 2018-01-23 | 2018-01-23 | A kind of T cell CAR developed by molecule analysis detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820111078.4U CN207933460U (en) | 2018-01-23 | 2018-01-23 | A kind of T cell CAR developed by molecule analysis detection kit |
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| CN207933460U true CN207933460U (en) | 2018-10-02 |
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| CN201820111078.4U Active CN207933460U (en) | 2018-01-23 | 2018-01-23 | A kind of T cell CAR developed by molecule analysis detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112545130A (en) * | 2020-11-26 | 2021-03-26 | 胡燕玲 | Portable reagent box for medical detection |
-
2018
- 2018-01-23 CN CN201820111078.4U patent/CN207933460U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112545130A (en) * | 2020-11-26 | 2021-03-26 | 胡燕玲 | Portable reagent box for medical detection |
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