CN207784037U - A kind of device for measuring low-oxygen environment and lasioderma serricorne growth and development being influenced - Google Patents
A kind of device for measuring low-oxygen environment and lasioderma serricorne growth and development being influenced Download PDFInfo
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- CN207784037U CN207784037U CN201820093192.9U CN201820093192U CN207784037U CN 207784037 U CN207784037 U CN 207784037U CN 201820093192 U CN201820093192 U CN 201820093192U CN 207784037 U CN207784037 U CN 207784037U
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Abstract
The utility model discloses a kind of device that measurement low-oxygen environment influences lasioderma serricorne growth and development, including experiment container, nitrogen cylinder, oxygen cylinder and detecting instrument, the experiment container includes the dryer door of drier and covering on the dryer, drier plug is fixed at the top of the dryer door, the drier is equipped with air inlet and test mouth beyond the Great Wall；The nitrogen cylinder and oxygen cylinder are connected to connection with air inlet after crossing respectively to mixing gas circuit by nitrogen pipeline and oxygen pipeline.There is the device experiment container of leakproofness to handle sample, while set temperature, humidity and oxygen content can be needed according to experiment, meet the requirement of experiment that in the environment of hypoxemia four lasioderma serricorne ovum, larva, pupa and adult worm states are carried out with bioassay.The device is easy to assembly, and performance is stablized, and disclosure satisfy that prolonged experiment demand.
Description
Technical field
The utility model is related to field of bioexperiment technology.More particularly, to a kind of measurement low-oxygen environment to lasioderma serricorne
The device that growth and development influences.
Background technology
Lasioderma serricorne, which is under the jurisdiction of, belongs to coleoptera, and Anobiidae endangers the tobacco leaf of storage in tobacco leaf warehouse, endangers in processing workshop
The evil tobacco products such as cigarette and cigar, huge economic loss is caused to tobacco industry, is the No.1 evil of worldwide tobacco storage
Worm.Lasioderma serricorne feeding habits are extensive, the grain that can also cause harm, tealeaves, beans, dry jujube, oil plant, animal or plant specimen, cocoa bean, leather and rattan
A variety of reserves such as bamboo product；Lasioderma serricorne can enter inner cigarette, moth food pipe tobacco with the pipe tobacco of processing, and moth wears cigarette paper, generation
Worm corpse, frass etc. can pollute tobacco product.
The domestic and international report studied stored product insect growth and development in relation to low-oxygen environment is more, is such as less than in oxygen content
In 1% environment, lethal saw-toothed grain beetle adult death needs the time of 1d or more, and lesser grain borer needs more than 4d, rice weevil then need be more than
14d, red flour beetle and confused flour beetle are required to 7d or more.Boring pest includes lesser grain borer, grain weevil and sitophilus oryzae to different oxygen
The patience of Gas content low-oxygen environment is generally more stronger than rusty grain beetle, saw-toothed grain beetle and red flour beetle.About low-oxygen environment to cigarette
It is less that the growth and development of the careless each worm state of first influences research.
Accordingly, it is desirable to provide a kind of device for measuring low-oxygen environment and lasioderma serricorne growth and development being influenced, to evaluate lasioderma serricorne
Each worm state to the ability of restraining oneself of low-oxygen environment, while carried out in the present apparatus in the environment of hypoxemia to lasioderma serricorne worm's ovum,
The hypoxemia tolerance test of 4 larva, pupa and adult worm states, data branch is provided for the practical application of hypoxemia Prevention Technique
It holds.
Utility model content
The purpose of this utility model is that it is to provide a kind of device that measurement low-oxygen environment influences lasioderma serricorne growth and development,
Set temperature, humidity and oxygen content can be needed according to experiment, and the experiment container with leakproofness handles sample, meet
In the environment of hypoxemia 4 lasioderma serricorne ovum, larva, pupa and adult worm states are carried out with the requirement of experiment of bioassay.
In order to achieve the above objectives, the utility model uses following technical proposals：
A kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development, which includes experiment container, nitrogen cylinder
And oxygen cylinder, the experiment container include the dryer door of drier and covering on the dryer, the top of the dryer door
It is fixed with drier plug, the drier is equipped with air inlet and test mouth beyond the Great Wall；The nitrogen cylinder and oxygen cylinder pass through respectively
Nitrogen pipeline and oxygen pipeline are connected to connection with air inlet after crossing to mixing gas circuit.
Preferably, further include drier collet in the experiment container, the drier collet is arranged in drier
Lower part, there are spaces between the drier collet and drier bottom wall.
Preferably, experimental solutions are equipped between the bottom wall of the drier and drier collet.
Preferably, the experimental solutions are saturated salt solution.According to the equilibrium relative humidity (ERH) table of saturated salt solution, prepare not
The saturated salt solution of required equilibrium relative humidity (ERH) when synthermal, to adjust the humidity in experiment container.
Preferably, the drier collet is equipped with laboratory sample.Laboratory sample is respectively lasioderma serricorne worm's ovum, lasioderma serricorne children
Worm, lasioderma serricorne pupa and lasioderma serricorne adult.
Preferably, the nitrogen pipeline is equipped with nitrogen flow control device, and the oxygen pipeline is equipped with oxygen flow
Control device.Nitrogen flow control device controls the nitrogen flow in nitrogen pipeline, and oxygen flux control device controls oxygen hose
Oxygen flow in road.
Preferably, the gaseous mixture road is equipped with detecting instrument.
Preferably, the detecting instrument is Orsat apparatus.Orsat apparatus can be with Accurate Analysis gaseous mixture
The ingredient and content of body.
Preferably, the detecting instrument is quick oxygen analyser.Quick oxygen analyser can be tested out quickly in mixed gas
Oxygen content.
Preferably, the experiment container is placed in incubator.The utility model using German binder incubators,
Experiment container is placed in the incubator to the temperature to be kept fixed.
The beneficial effects of the utility model are as follows：
As a result of above technical scheme, which there is the utility model the experiment container of leakproofness to handle sample,
Set temperature, humidity and oxygen content can be needed according to experiment simultaneously, is met in the environment of hypoxemia to lasioderma serricorne ovum, children
Four worm, pupa and adult worm states carry out the requirement of experiment of bioassay.The device is easy to assembly, and performance is stablized, and disclosure satisfy that length
The experiment demand of time.
Description of the drawings
Specific embodiment of the present utility model is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 shows a kind of structural representation measuring the device that low-oxygen environment influences lasioderma serricorne growth and development of the utility model
Figure.
Under the conditions of relative humidity 75% in the utility model experimental result, 2% oxygen content handles cigarette beetle
The relationship of ovum 99% dead time and temperature.
Each reference numeral meaning in attached drawing：1 drier, 2 dryer doors, 3 drier plugs, 4 drier collets, 5 experiments
Solution, 6 air inlets, 7 test mouths, 8 nitrogen cylinders, 9 nitrogen flow control devices, 10 oxygen cylinders, 11 oxygen flux control devices, 12
Detecting instrument, 13 laboratory samples.
Specific implementation mode
In order to illustrate more clearly of the utility model, the utility model is done into one with reference to preferred embodiments and drawings
The explanation of step.Similar component is indicated with identical reference numeral in attached drawing.It will be appreciated by those skilled in the art that below
Specifically described content is illustrative and be not restrictive, and should not limit the scope of protection of the utility model with this.
As shown in Figure 1, a kind of device for measuring low-oxygen environment and lasioderma serricorne growth and development being influenced, including experiment container, nitrogen
Gas cylinder 8, oxygen cylinder 10 and detecting instrument 12, the experiment container include drier 1 and the dryer door that is covered on drier 1
2, the top of the dryer door 2 is fixed with drier plug 3, and the drier plug 3 is equipped with air inlet 6 and test mouth 7.Institute
It further includes drier collet 4 to state in experiment container, and the drier collet 4 is arranged in the middle and lower part of drier 1, the drier
There are spaces between 1 bottom wall of collet 4 and drier.
Experimental solutions 5 are equipped between the bottom wall and drier collet 4 of the drier 1.
The experimental solutions 5 are saturated salt solution.According to the equilibrium relative humidity (ERH) table of saturated salt solution, different temperatures is prepared
The saturated salt solution of Shi Suoxu equilibrium relative humidity (ERH)s, to adjust the humidity in experiment container.
The drier collet 4 is equipped with laboratory sample 13.Laboratory sample 13 is respectively lasioderma serricorne worm's ovum, lasioderma serricorne children
Worm, lasioderma serricorne pupa and lasioderma serricorne adult.
The experiment container is placed in incubator.The utility model will be tested using German binder incubators
Container is placed on the temperature to be kept fixed in the incubator.
The nitrogen cylinder 8 and oxygen cylinder 10 crossed respectively to mixing gas circuit by nitrogen pipeline and oxygen pipeline after with air inlet
6 connection connection of mouth.The nitrogen pipeline is equipped with nitrogen flow control device 9, and the oxygen pipeline is equipped with oxygen flow control
Device 11 processed.Nitrogen flow control device controls the nitrogen flow in nitrogen pipeline, and oxygen flux control device controls oxygen hose
Oxygen flow in road.
The gaseous mixture road is equipped with detecting instrument 12.
The detecting instrument 12 is Orsat apparatus.Orsat apparatus can with Accurate Analysis mixed gas at
Point and content.
The detecting instrument 12 is quick oxygen analyser.The oxygen that quick oxygen analyser can quickly test out in mixed gas contains
Amount.
The application method and reality for the device that a kind of measurement low-oxygen environment of the utility model influences lasioderma serricorne growth and development
It is as follows to test process：
1. the air-leakage test of drier 1：Drier 1, dryer door 2 and drier plug 3 are cleaned with deionized water, dried
It is dry, after it is fully cooled, all junctions are uniformly smeared with vaseline.U-tube manometer is connected in constant temperature and pressure environment
Onto air inlet 6, it is filled with appropriate air into drier 1 by testing mouth 7, makes to wait for for positive pressure 1000Pa or so in drier 1
Pressure value is recorded after pressure stability, for 24 hours after, if pressure is unchanged, this drier 1 can be used to test.
2. oxygen concentration is adjusted：Test mouth 7 is closed, the nitrogen cylinder 8 and oxygen cylinder 10 pass through nitrogen pipeline and oxygen respectively
Air pipe is connected to connection with air inlet 6 after crossing to mixing gas circuit, gaseous mixture road setting Orsat apparatus is surveyed with quick
Oxygen instrument.Nitrogen flow control device 9 is set to be constant value, oxygen flux control device 11 is adjusted, uses Orsat apparatus
The oxygen concentration for detecting mixed gas, when mixed gas oxygen concentration is pre-set aimed concn, with quick oxygen analyser
The oxygen concentration of the mixed gas is detected to calibrate quick oxygen analyser, and records the gas of connection nitrogen cylinder 8 and oxygen cylinder 10
The reading of flow flowmeter.After setting gas flowmeter, start to be passed through gaseous mixture into the drier 1 for meeting air-tightness requirement
Body, while being stopped when the oxygen concentration of air inlet 6 reaches setting value with the oxygen concentration at quick oxygen analyser detection air inlet 6
Inflate and close the valve of the valve and oxygen cylinder 10 of nitrogen cylinder 8.
3. temperature and humidity controls：Experiment container is placed in German binder incubators to control temperature；According to full
With the equilibrium relative humidity (ERH) table of salting liquid, the saturated salt solution of required equilibrium relative humidity (ERH) when different temperatures is prepared, drying is placed in
Space between 1 bottom wall of device collet 4 and drier, to adjust humidity.
4. test method：
The processing and observation of 4.1 lasioderma serricorne worm's ovums
By 20 (1 day age) more full primiparity lasioderma serricorne worm's ovum uniformly dispersing the 20mm for being stained with double faced adhesive tape ×
On the coverslip of 20mm, ovum card is made, 3 ovum cards are 1 group and are pasted onto on glass slide, and it is low to be placed on progress in above-mentioned experiment container
Oxygen processing；Blank control is done in other 3 identical ovum card placements in a natural environment.Treated that ovum is placed in that experiment is held for hypoxemia
It cultivates under different experiment conditions in device, observes daily, until all dead or Fu chemical conversions larva stops observation to lasioderma serricorne worm's ovum,
And it records.
4.2 the processing and observation of lasioderma serricorne larva
Diameter 15mm, the lucite pipe of length 50mm, tube wall surrounding is taken to prick out 50 apertures with needle, be packed into plastic tube
Appropriate lasioderma serricorne feed, makes test worm cage, each test worm cage be put into 30 ages in days, uniformity of type lasioderma serricorne larva.3 test worms
Cage is 1 group and is placed on progress hypoxemia processing in above-mentioned experiment container, and in addition 3 identical test worm cages placements are done in a natural environment
Blank control.Treated that test worm cage is placed in experiment container is cultivated under different experimental conditions for hypoxemia, observes daily, until all cigarettes
Careless first larva sprouts wings or stops observation when dead, and records.
The processing and observation of 4.3 lasioderma serricorne pupas
Appropriate lasioderma serricorne feed is packed into test worm cage, it is young that each worm cage is put into 30 ages in days, 4 age lasioderma serricornes of uniformity of type
Worm, it is to wait for that it is pupated in one week or so in the environment that 30 DEG C of relative humidity are 75% to be put into temperature.3 test worm cages are 1 group and are placed on
Hypoxemia processing is carried out in above-mentioned experiment container, blank control is done in addition 3 identical test worm cage placements in a natural environment.Hypoxemia
Treated, and test worm cage is placed under experiment condition different in experiment container cultivates, and observes daily, to the emergence of all lasioderma serricorne pupas
Or stop observation when dead, and record.
The processing and observation of 4.4 lasioderma serricorne adults
The tobacco leaf that a small pieces moisture is 14% is packed into test worm cage, placed in each worm cage sprout wings 2 days it is more active at
Worm 30.3 test worm cages are 1 group and are placed on progress hypoxemia processing in above-mentioned experiment container, and in addition 3 identical test worm cages are placed
Blank control is done in a natural environment.Treated that test worm cage is placed under experiment condition different in experiment container cultivates for hypoxemia,
It observes afterwards for 24 hours, takes out lasioderma serricorne adult, touched one by one with writing brush under stereomicroscope, inactive person is determined as death.
5. data statistics
Toxicity regression curve is calculated using PROC PROBIT, finds out LD50 time LT50 and 99% lethal time
The confidence limit of LT99 and its 95%, b values and its standard error, between different worm states the comparison of LT50 and b values μ tests are respectively adopted
It is test with t.
6. experimental result and analysis
Function and effect of 6.1 low-oxygen environments to lasioderma serricorne worm's ovum
Under conditions of relative humidity 75%, within the scope of 15-35 DEG C of temperature, 2% oxygen content has lasioderma serricorne worm's ovum
Good killing effect obtains good bioassay equation (being shown in Table 1).Temperature handles hypoxemia the effect of lasioderma serricorne worm's ovum
There is significant impact, from the point of view of under different temperatures to lasioderma serricorne worm's ovum lethal time and its confidence interval size, temperature is higher, right
Lasioderma serricorne worm's ovum hypoxemia treatment effect is better.Low temperature can reduce the effect of hypoxemia processing lasioderma serricorne worm's ovum.20 DEG C or more, 2% oxygen
Gas content processing lasioderma serricorne worm's ovum can reach 99% or more the death rate in 10 days.Therefore, cigarette beetle is handled using hypoxemia
When ovum, temperature is unsuitable too low, it is proposed that 25 DEG C or more are handled.
Table 1-2% oxygen contents (15-35 DEG C of relative humidity 75%, temperature) are to the treatment effect of lasioderma serricorne worm's ovum
It is illustrated in figure 2 under the conditions of relative humidity 75%, 2% oxygen content handles the dead time of lasioderma serricorne worm's ovum 99%
With the relationship of temperature, it can be seen that the time for lasioderma serricorne worm's ovum reaches 99% death rate that treated under this condition, with temperature
The reduction of degree linearly increases, and temperature often reduces by 1 DEG C, and hypoxemia processing time accordingly increases by 0.48 day, i.e., about 12h.
Function and effect of 6.2 low-oxygen environments to lasioderma serricorne larva and pupa
In 30 DEG C of temperature, relative humidity 75%, 2% oxygen, under 98% condition of nitrogen gas, hypoxemia handles lasioderma serricorne larva and pupa
10 days, 100% death rate can be reached.Larva bioassay equation is：Y=9.3290lg (x)
- 6.8469, LD50 time LT50 is 5.42 days, and 95% confidence interval is 5.18~5.66 days, and LT99 is
9.62 days, 95% confidence interval was 8.86~10.74 days, refers to table 2.Pupa bioassay equation is：Y=7.71g (x)-
4.67, LD50 time LT50 are 4.03 days, and 95% confidence interval is 3.71~4.29 days, and LT99 is 8.07 days, 95%
Confidence interval be 7.22~9.58 days, refer to table 2.
Table 2-2% oxygen contents (30 DEG C of relative humidity 75%, temperature) imitate the processing of lasioderma serricorne larva and lasioderma serricorne pupa
Fruit
Function and effect of 6.3 low-oxygen environments to lasioderma serricorne adult
From table 3 it can be seen that 30 DEG C of temperature, relative humidity 75%, under the conditions of oxygen content 2%, hypoxemia handles lasioderma serricorne
Adult, bioassay equation are：Y=2.9211lg (x) -2.1930, wherein LD50 time LT50 are 5.63 days, 95%
Confidence interval is 4.92-6.66 days, and LT99 is 35.25 days, and 95% confidence interval is 22.23-79.56 days, refers to table 3.
35 DEG C of temperature, relative humidity 75%, under the conditions of oxygen content 2%, hypoxemia handles lasioderma serricorne adult, bioassay side
Cheng Wei：Y=3.1233lg (x) -1.1966, wherein LD50 time LT50 are 2.42 days, and 95% confidence interval is 1.76-
3.02 days, LT99 was 13.43 days, and 95% confidence interval is 8.32-40.34 days, refers to table 3.
Table 3-2% oxygen contents (25-35 DEG C of relative humidity 75%, temperature) are to the treatment effect of lasioderma serricorne adult
When same oxygen concentration and relative humidity, under the conditions of 25 DEG C of temperature, 2 measurement have been carried out, the results showed that, 2% oxygen
Gas content is analyzed by Probit methods to lasioderma serricorne adult lethal data, cannot obtain bioassay equation, be shown in Table 3.
It finds, through hypoxemia treated adult, to have no oviposition, that is, there is no F1 generation lasioderma serricorne in above-mentioned experiment.
7. experiment conclusion
Hypoxemia has good killing effect to each worm state of lasioderma serricorne.Under the conditions of 30 DEG C of temperature, relative humidity 75%,
2% oxygen content handles in 10 days lasioderma serricorne worm's ovum, lasioderma serricorne larva, lasioderma serricorne pupa, can reach 99% or more death
Rate, the time lethal to lasioderma serricorne adult 99% is about at 22 days.But repeatedly the test result range of variation of adult is larger, needs
Further research verification.
Each worm state of lasioderma serricorne is different to the sensibility of hypoxemia.According to the comparison of median lethal time (LT50), four kinds of lasioderma serricorne
The sensitive sequence of the oxygen content of worm state pair 2% is：Ovum, adult, pupa and larva；According to LT99, four kinds of worm states pair 2% of lasioderma serricorne
The sensitive sequence of oxygen content be：Ovum, pupa, larva, adult.
The effect of 2% oxygen content of temperature pair processing lasioderma serricorne ovum has significant impact.Low temperature can reduce hypoxemia processing cigarette
The effect of careless beetle ovum, under conditions of relative humidity 75%, temperature often lowers 1 DEG C, and 2% oxygen content processing lasioderma serricorne ovum reaches
99% lethal time needed corresponding extension 0.48 day.The influence that temperature handles other worm state hypoxemia needs further to be studied.
Obviously, above-described embodiment of the utility model is only intended to clearly illustrate the utility model example, and simultaneously
Non- is the restriction to the embodiment of the utility model, for those of ordinary skill in the art, in above description
On the basis of can also make other variations or changes in different ways, all embodiments can not be exhaustive here, it is all
It is the guarantor for belonging to obvious changes or variations that the technical solution of the utility model is extended out still in the utility model
Protect the row of range.
Claims (10)
1. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development, which is characterized in that the device includes that experiment is held
Device, nitrogen cylinder (8) and oxygen cylinder (10), the experiment container include drier (1) and the drier that is covered on drier (1)
It covers (2), drier plug (3) is fixed at the top of the dryer door (2), the drier plug (3) is equipped with air inlet (6)
With test mouth (7)；
The nitrogen cylinder (8) and oxygen cylinder (10) crossed respectively to mixing gas circuit by nitrogen pipeline and oxygen pipeline after with air inlet
Mouth (6) connection connection.
2. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 1, feature exist
In further including drier collet (4) in the experiment container, under the drier collet (4) is arranged in drier (1)
Portion, there are spaces between the drier collet (4) and drier (1) bottom wall.
3. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 2, feature exist
In equipped with experimental solutions (5) between the bottom wall and drier collet (4) of the drier (1).
4. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 3, feature exist
In the experimental solutions (5) are saturated salt solution.
5. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 4, feature exist
In the drier collet (4) is equipped with laboratory sample (13).
6. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 1, feature exist
In the nitrogen pipeline is equipped with nitrogen flow control device (9), and the oxygen pipeline is equipped with oxygen flux control device
(11)。
7. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 6, feature exist
In the gaseous mixture road is equipped with detecting instrument (12).
8. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 7, feature exist
In the detecting instrument (12) is Orsat apparatus.
9. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 7, feature exist
In the detecting instrument (12) is quick oxygen analyser.
10. a kind of device for measuring low-oxygen environment and being influenced on lasioderma serricorne growth and development according to claim 5, feature exist
In the experiment container is placed in incubator.
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