CN207780033U - Anaphylactogen micro-fluid chip detector - Google Patents
Anaphylactogen micro-fluid chip detector Download PDFInfo
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- CN207780033U CN207780033U CN201820008540.8U CN201820008540U CN207780033U CN 207780033 U CN207780033 U CN 207780033U CN 201820008540 U CN201820008540 U CN 201820008540U CN 207780033 U CN207780033 U CN 207780033U
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- Prior art keywords
- pdms
- microfluid
- anaphylactogen
- micro
- fluid chip
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- 239000012530 fluid Substances 0.000 title claims abstract description 22
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- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims abstract description 42
- 235000013870 dimethyl polysiloxane Nutrition 0.000 claims abstract description 41
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 238000001514 detection method Methods 0.000 claims abstract description 38
- 210000004369 blood Anatomy 0.000 claims abstract description 24
- 239000008280 blood Substances 0.000 claims abstract description 24
- 238000006243 chemical reaction Methods 0.000 claims abstract description 24
- 238000001914 filtration Methods 0.000 claims abstract description 20
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- 210000004027 cell Anatomy 0.000 description 9
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- 210000002966 serum Anatomy 0.000 description 3
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 208000026935 allergic disease Diseases 0.000 description 2
- 230000000172 allergic effect Effects 0.000 description 2
- 230000007815 allergy Effects 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229940127121 immunoconjugate Drugs 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model is related to anaphylactogen micro-fluid chip detectors, the blood filtration for including microfluid PDMS chips and being from left to right implanted into connection successively carries seat, sample collection dividing cell, EGR Delay Valve, detect antibody units, reaction member, capillary pump, output channel and fluorescence reading machine, microfluid PDMS chips are the PDMS membrane DNA chips using Si as base material, it is siphon loading pad that blood filtration, which carries seat, at least 15 groups of sample collection dividing cell compartment of terrain carry seat with the blood filtration and are connected, at least 15 groups of detection group units are arranged in width direction along microfluid PDMS, the output channel is connect with the fluorescence reading machine.The anaphylactogen micro-fluid chip detector of the utility model, using microfluid PDMS chips, one bleed siphon shunting, at least 15 groups of detection group units, every group obtains a corresponding anaphylactogen index, capillary pump microfluid, data are read automatically by reading machine, and test speed is fast, as a result objective, accurate, at low cost, synthesis is cost-effective.
Description
Technical field
The utility model is related to technical field of medical equipment, the especially quick detection device of anaphylactogen micro-fluid chip.
Background technology
Human allergy's original detects, and the Allergic skin test of the prior art generally uses enzyme immunoassay, to patients serum or blood plasma
In anaphylactogen (total IgE, total IgG, specific IgE etc.) carry out qualitative and quantitative detection.It generally will be first by serum and haemocyte
Anaphylactogen reagent strip is added in detection serum by separation, to detect last as a result, whole process is comparatively laborious, the blood volume needed
Greatly, a large amount of reagent is wasted, is easy to be affected by human factors；And the reaction time is long, whole process take around 3-4 hours it is complete
At.And the detection of anaphylactogen is detected using uniCAP, it is the gold standard of Allergic skin test, though it is whole complete by machine
At, reduce human error, but its reaction time be not also obviously shortened；And it detects machine and consumables cost is high, can detect
Quick original seed class is relatively limited, is unfavorable for base's popularization.
Utility model content
Based on this, for the prior art, the technical problem to be solved of the utility model is just to provide a kind of quickly inspection
It surveys, the anaphylactogen micro-fluid chip detector of low cost, high accuracy.
The technical solution of the utility model is：
A kind of anaphylactogen micro-fluid chip detector, including microfluid PDMS chips, blood filtration carry seat, sample collection point
Flow unit, EGR Delay Valve, detection antibody units, reaction member, capillary pump, output channel and fluorescence reading machine, the microfluid
PDMS chips are using Si as the micro-fluid chip of the PDMS cover films of base material, in the microfluid PDMS chips from left to right successively
The blood filtration of implantation connection carries seat, sample collection dividing cell, EGR Delay Valve, detection antibody units, reaction member, capillary
Pump and output channel, it is that siphon loading pad is added dropwise in blood to be measured that the blood filtration, which carries seat, and the blood filtration carries seat as along institute
The width setting serial siphon for stating microfluid PDMS chips carries seat, at least sample collection dividing cell compartment of terrain and institute described in 15 groups
State blood filtration carry seat conducting, the sample collection dividing cell, EGR Delay Valve, detection antibody units, reaction member, capillary pump,
Output channel is the length extension composition detection group unit along the microfluid PDMS chips, along the microfluid PDMS chips
Width direction setting at least detection group unit described in 15 groups, the output channel is connect with the fluorescence reading machine.
The EGR Delay Valve bandwidth of the EGR Delay Valve is 40~50 μm in one of the embodiments,.
The detection antibody units include flow resistance and detection antibody deposition area, the detection in one of the embodiments,
Antibody deposition area is to be located among two flow resistances of two concatenations conducting, flow resistance between two flow resistances conducting line width is 85~
95 μm, the antibody deposition area and the deposition line width of the flow resistance conductive line detected is 50~55 μm.
In one of the embodiments, the reaction line width of the reaction member be 25~30 μm, including capture antibody line and
Control line.
The microfluid PDMS chips include Si chip substrates and PDMS mask lids in one of the embodiments, described
The bottom surface of PDMS mask lids is implanted into the sample collection dividing cell, EGR Delay Valve, detection antibody units, reaction member, capillary pump
And output channel, the PDMS masks lid lid are located in the Si chip substrates.
The EGR Delay Valve is the coated fluorescent grain of anaphylactogen or magnetic bead in one of the embodiments,.
Compared with the prior art, the anaphylactogen micro-fluid chip detector of the utility model, using microfluid PDMS chips,
One bleeds siphon shunting, and at least 15 groups of detection group units, every group obtains a corresponding anaphylactogen index, capillary pump microfluid, logical
It crosses reading machine and reads data automatically, test speed is fast, as a result objective, accurate, at low cost, and synthesis is cost-effective.
Description of the drawings
Fig. 1 is the structural schematic diagram of the anaphylactogen micro-fluid chip detector of the utility model；
Fig. 2 is the reaction process schematic diagram of the allergy fluid detection of the utility model.
In figure, 10-- microfluid PDMS chips；11--Si chip substrates；12--PDMS mask lids；20-- blood filtrations carry
Seat；30-- sample collection dividing cells；40-- EGR Delay Valves；50-- detects antibody units；51-- flow resistances；It is heavy that 52-- detects antibody
Product area；60-- reaction members；61-- captures antibody line；62-- control lines；70-- capillary pumps；80-- exports hole path；α -- prolong
Slow valve bandwidth；Line width is connected in β -- flow resistance；λ -- deposition line is wide；γ -- reaction line width；A-- reactants to be measured；B-- detections are anti-
Body；C-- captures antibody.
Specific implementation mode
Below with reference to the accompanying drawings and in conjunction with the embodiments the utility model is described in detail.It should be noted that not rushing
In the case of prominent, the feature in following embodiment and embodiment can be combined with each other.
Fig. 1 gives the anaphylactogen micro-fluid chip detector of the utility model, and Fig. 2 is reaction process schematic diagram, by scheming
It is found that the anaphylactogen micro-fluid chip detector, including microfluid PDMS chips 10, blood filtration carry seat 20, sample collection point
Unit 30, EGR Delay Valve 40, detection antibody units 50, reaction member 60, capillary pump 70, output channel 80 and fluorescence reading machine are flowed,
Microfluid PDMS chips 10 be using Si as the micro-fluid chip of the PDMS cover films of base material, in microfluid PDMS chips 10 from a left side to
The blood filtration that the right side is implanted into connection successively carries seat 20, sample collection dividing cell 30, EGR Delay Valve 40, detects antibody units 50, is anti-
Unit 60, capillary pump 70 and output channel 80 are answered, it is that siphon loading pad, blood filtration is added dropwise in blood to be measured that blood filtration, which carries seat 20,
Seat 20 is carried as between the width of microfluid PDMS chips 10 setting serial siphon load seat, at least 15 groups of sample collection dividing cells 30
Seat 20 is carried every ground and blood filtration to be connected, sample collection dividing cell 30, EGR Delay Valve 40, detection antibody units 50, reaction member
60, capillary pump 70, output channel 80 are the length extension composition detection group unit along microfluid PDMS chips 10, along microfluid
Width direction setting at least 15 groups of detection group units, the output channel 80 of PDMS chips 10 is connect with fluorescence reading machine.
The EGR Delay Valve bandwidth α of EGR Delay Valve 40 is 40~50 μm.It includes that flow resistance 51 and detection antibody are heavy to detect antibody units 50
Product area 52, detection antibody deposition area 52 are to be located among two flow resistances 51 of two concatenations conducting, the flow resistance conducting between two flow resistances 51
Line width β is 85~95 μm, and the wide λ of deposition line of detection antibody deposition area 52 and flow resistance conductive line is 50~55 μm.Reaction member
60 reaction line width γ is 25~30 μm, including capture antibody line 61 and control line 62.
Microfluid PDMS chips 10 include Si chip substrates 11 and PDMS masks lid 12, and the bottom surface of PDMS masks lid 12 is implanted into
Sample collection dividing cell 30, EGR Delay Valve 40, detection antibody units 50, reaction member 60, capillary pump 70 and output channel 80,
The lid of PDMS masks lid 12 is located in Si chip substrates 11.
EGR Delay Valve 40 is that EGR Delay Valve is the coated fluorescent grain of anaphylactogen or magnetic bead.
Blood filtration carries the reactant A molecule to be measured of seat 20 when flowing through detection antibody units 50, point with detection antibody B
Sub- reaction bonded obtains antibody conjugates.The molecule of antigen-antibody conjugate, when flowing through reaction member 60, be captured antibody C
Molecule trapping.
From the above, it may be appreciated that the anaphylactogen micro-fluid chip detector of the utility model, using microfluid PDMS chips, one
Siphon of bleeding shunts, and at least 15 groups of detection group units, every group obtains a corresponding anaphylactogen index, and capillary pump microfluid passes through
Reading machine reads data automatically, and test speed is fast, as a result objective, accurate, at low cost, and synthesis is cost-effective.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the common of this field
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In the scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (6)
1. a kind of anaphylactogen micro-fluid chip detector, which is characterized in that including microfluid PDMS chips, blood filtration carry seat,
Sample collection dividing cell, EGR Delay Valve, detection antibody units, reaction member, capillary pump, output channel and fluorescence reading machine, institute
It is using Si as the micro-fluid chip of the PDMS cover films of base material, from a left side in the microfluid PDMS chips to state microfluid PDMS chips
The blood filtration for being implanted into connection successively to the right side carries seat, sample collection dividing cell, EGR Delay Valve, detection antibody units, reaction
Unit, capillary pump and output channel, it is that siphon loading pad is added dropwise in blood to be measured that the blood filtration, which carries seat, and the blood filtration carries
Seat carries seat for serial siphon is arranged along the width of the microfluid PDMS chips, at least between sample collection dividing cell described in 15 groups
Every ground and the blood filtration seat is carried be connected, the sample collection dividing cell, EGR Delay Valve, detect antibody units, reaction member,
Capillary pump, output channel are the length extension composition detection group unit along the microfluid PDMS chips, along the microfluid
The width direction setting at least detection group unit described in 15 groups of PDMS chips, the output channel connect with the fluorescence reading machine
It connects.
2. anaphylactogen micro-fluid chip detector according to claim 1, which is characterized in that the EGR Delay Valve of the EGR Delay Valve
Bandwidth is 40~50 μm.
3. anaphylactogen micro-fluid chip detector according to claim 1, which is characterized in that the detection antibody units packet
Flow resistance and detection antibody deposition area are included, the detection antibody deposition area is to be located among two flow resistances of two concatenations conducting, two
Flow resistance conducting line width between the flow resistance is 85~95 μm, the deposition in detection the antibody deposition area and the flow resistance conductive line
Line width is 50~55 μm.
4. anaphylactogen micro-fluid chip detector according to claim 1, which is characterized in that the reaction of the reaction member
Line width is 25~30 μm, including capture antibody line and control line.
5. according to Claims 1 to 4 any one of them anaphylactogen micro-fluid chip detector, which is characterized in that the miniflow
Body PDMS chips include Si chip substrates and PDMS mask lids, and the bottom surface of the PDMS masks lid is implanted into the sample collection shunting
Unit, EGR Delay Valve, detection antibody units, reaction member, capillary pump and output channel, the PDMS masks lid lid are located at the Si
In chip substrates.
6. anaphylactogen micro-fluid chip detector according to claim 5, which is characterized in that the EGR Delay Valve is to detect
The fluorescent grain or magnetic bead of quick primordial covering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820008540.8U CN207780033U (en) | 2018-01-03 | 2018-01-03 | Anaphylactogen micro-fluid chip detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820008540.8U CN207780033U (en) | 2018-01-03 | 2018-01-03 | Anaphylactogen micro-fluid chip detector |
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| Publication Number | Publication Date |
|---|---|
| CN207780033U true CN207780033U (en) | 2018-08-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820008540.8U Expired - Fee Related CN207780033U (en) | 2018-01-03 | 2018-01-03 | Anaphylactogen micro-fluid chip detector |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843729A (en) * | 2018-01-03 | 2018-03-27 | 洪海裕 | Anaphylactogen micro-fluid chip detector |
-
2018
- 2018-01-03 CN CN201820008540.8U patent/CN207780033U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843729A (en) * | 2018-01-03 | 2018-03-27 | 洪海裕 | Anaphylactogen micro-fluid chip detector |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201013 Address after: 519000 collective dormitory of No. 52 Meihua East Road, Xiangzhou City, Zhuhai City, Guangdong Province Patentee after: Hong Haiyu Patentee after: SUN YAT-SEN University Patentee after: THE FIFTH AFFILIATED HOSPITAL OF SUN YAT-SEN University Address before: The big five Mei Xiangzhou District of Xiangzhou city of Zhuhai province Guangdong 519000 China Road No. 52 in the dormitory Patentee before: Hong Haiyu |
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| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180828 |
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| CF01 | Termination of patent right due to non-payment of annual fee |