CN207738757U - Umbilical cord stem cells extraction element - Google Patents
Umbilical cord stem cells extraction element Download PDFInfo
- Publication number
- CN207738757U CN207738757U CN201721881397.5U CN201721881397U CN207738757U CN 207738757 U CN207738757 U CN 207738757U CN 201721881397 U CN201721881397 U CN 201721881397U CN 207738757 U CN207738757 U CN 207738757U
- Authority
- CN
- China
- Prior art keywords
- umbilical cord
- shell
- stem cells
- cord stem
- extraction element
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of umbilical cord stem cells extraction elements, are related to stem cell extraction field, including destroyer and extraction vessel, it is characterised in that：The destroyer includes shell, and shell one end is connect with the extraction vessel, and shell cover is arranged in the other end, and the shell cover is equipped with through-hole, and shell cover is equipped with multiple columnar projections towards shell face；It further include drawing device, the drawing device includes pulling part, lifting rod and broken portion, lifting rod passes through the through-hole, it is installed with broken net in broken portion, the extraction process for realizing stem cell provides a gnotobasis, carry out umbilical cord stem cells extraction that can not only be quick and easy, and the effect using concentration of mycillin can be reduced.
Description
Technical field
The utility model is related to stem cell extractive technique field, especially a kind of umbilical cord stem cells extraction element.
Background technology
Mescenchymal stem cell (MSC) be one kind in marrow other than candidate stem cell (HSC) have height self more
New and multi-lineage potential stem cell.Under different inductive conditions, the tissue that can be divided into other than a variety of hematopoietic cells is thin
Born of the same parents, and have the effects that hematopoiesis support, immunological regulation, tissue repair.Previous mescenchymal stem cell is mainly derived from marrow, but with
The aging at age, mescenchymal stem cell gradually decreases, and Proliferation, Differentiation ability declines significantly, and materials damage donor, limits
The application of mesenchymal stem cell is made.A kind of alternative source newly is found as compeling highly necessary to solve the problems, such as.
Mescenchymal stem cell can be separately cultured out by reporting for the first time within 2000 in Cord blood, be then divided into not from umbilical vein
It has been separately cultured out mescenchymal stem cell in endothelium and subendothelial, umbilical cord Fahrenheit colloid and tissues surrounding vascular, and has established navel
Isolated culture method with mescenchymal stem cell.Brand-new conceptual phase is entered to the stem cell extraction of umbilical cord from this people.
Stem cell extraction in usual umbilical cord removes blood stains using phosphate buffer (PBS) surface washing, after clean umbilical cord is shredded
It is dipped in after being handled in clostridiopetidase A, then gives trypsin digestion processing, liquid phase obtains targeted stem cells by centrifugation.
The prior art can not prevent the biological pollution in extraction process, it is therefore desirable to the mycillin of higher concentration is used,
But the use of the mycillin of high concentration can reduce going back to the nest and Adhesion property in vivo for umbilical cord MSC.Be unfavorable for the later stage scientific research and
It uses.
Utility model content
The utility model provides a kind of umbilical cord stem cells extraction element, and an asepsis ring is provided for the extraction process of stem cell
Border, carry out umbilical cord stem cells extraction that can not only be quick and easy, and the use concentration of mycillin can be reduced.
To realize above-mentioned technique effect, the utility model discloses a kind of umbilical cord stem cells extraction elements, including destroyer
And extraction vessel, wherein:The destroyer includes shell, and shell one end is connect with the extraction vessel, and shell cover is arranged in the other end,
The shell cover is equipped with through-hole, and shell cover is equipped with multiple columnar projections towards shell face；Further include drawing device, the drawing device includes
Pulling part, lifting rod and broken portion, lifting rod pass through the through-hole, broken net are installed in broken portion.
Further, the upper opening of the shell is more than under shed.
Further, the shell is detachably connected with extraction vessel, is detachably connected between shell and shell cover.
Further, the extraction vessel is polytetrafluoroethylene (PTFE) material.
Further, the columnar projections on the inside of the shell cover be rectangular array, the line space of the columnar projections with
Column pitch is identical.
Further, wherein the broken portion is square or rectangular frame, quadrangle is respectively equipped with vertical with frame carry
Pull rod, lifting rod end are fixedly connected with pulling part.
Further, wherein the shell cover is set, there are four through-holes.
Further, wherein：The broken net is square mesh structure, and the broken net meshe is matched with columnar protrusions gap
It closes.
Further, wherein：The material of the broken net is high strength and modulus fiber.
Further, wherein：The shell side is equipped with liquid injection hole.
The extraction element of a kind of umbilical cord stem cells disclosed by the utility model, by the way that umbilical cord extraction sample is placed in broken portion
It is broken online, by lifting pulling part, by the extruding of columnar projections, umbilical cord is made to be broken the broken container for falling into lower section of net
In, enzyme solutions are injected passing through liquid injection hole, realize the simple and quick stem cell extraction of umbilical cord, and it is dry that whole process is totally pollution-free
The extraction process of cell provides a gnotobasis, can reduce the good result using concentration of mycillin.
Description of the drawings
Fig. 1 the utility model umbilical cord stem cells extraction element schematic diagrames.
Fig. 2 the utility model destroyer structural schematic diagrams.
The vertical view of Fig. 3 the utility model shell covers.
The stereogram of Fig. 4 the utility model drawing devices.
1. 6 drawing device of destroyer 2. extraction vessel, 3. shell, 4. shell cover, 5. columnar projections, 61. pulling part 62. carries
Pull rod 63. is crushed portion 64. and is crushed 7. through-hole of net, 8. liquid injection hole.
Specific implementation mode
Umbilical cord stem cells extraction element disclosed by the utility model is specifically solved below in conjunction with specific embodiment
Release explanation.
Embodiment one, a kind of umbilical cord stem cells extraction element, including destroyer and extraction vessel, it is characterised in that：It is described
Destroyer includes shell, and shell one end is connect with the extraction vessel, and shell cover is arranged in the other end, and the shell cover is equipped with through-hole,
Shell cover is equipped with multiple columnar projections towards shell face；Further include drawing device, the drawing device includes pulling part, lifting rod and is crushed
Portion, lifting rod pass through the through-hole, broken net are installed in broken portion.
When in use, umbilical cord sample is placed in the broken online of broken portion, lifting pulling part makes umbilical cord pass through columnar projections
Extruding, so that umbilical cord is broken net attrition crushing into fritter, fall into the extraction vessel of lower section.
Embodiment two, a kind of umbilical cord stem cells extraction element, including destroyer and extraction vessel, it is characterised in that：It is described
Destroyer includes shell, and shell one end is connect with the extraction vessel, and shell cover is arranged in the other end, and the shell cover is equipped with through-hole,
Shell cover is equipped with multiple columnar projections towards shell face；Further include drawing device, the drawing device includes pulling part, lifting rod and is crushed
Portion, lifting rod pass through the through-hole, broken net are installed in broken portion.Shell side is equipped with liquid injection hole.
When in use, umbilical cord sample is placed in the broken online of broken portion, lifting pulling part makes umbilical cord pass through columnar projections
Extruding, so that umbilical cord is broken net attrition crushing into fritter, fall into the extraction vessel of lower section.By the liquid injection hole of side, it is added
Enzyme preparation extracts stem cell.
Embodiment three, a kind of umbilical cord stem cells extraction element, including destroyer and extraction vessel, it is characterised in that：It is described
Destroyer includes shell, and shell one end is connect with the extraction vessel, and shell cover is arranged in the other end, and the shell cover is equipped with through-hole,
Shell cover is equipped with multiple columnar projections towards shell face；Further include drawing device, the drawing device includes pulling part, lifting rod and is crushed
Portion, lifting rod pass through the through-hole, broken net are installed in broken portion.Shell side is equipped with liquid injection hole.Extraction vessel is poly- four
Vinyl fluoride material.
When in use, umbilical cord sample is placed in the broken online of broken portion, lifting pulling part makes umbilical cord pass through columnar projections
Extruding, so that umbilical cord is broken net attrition crushing into fritter, fall into the extraction vessel of lower section.By the liquid injection hole of side, it is added
Enzyme preparation extracts stem cell, and after extraction, cell residue does not end up on extraction vessel wall, and cleaning is simple.
Claims (10)
1. umbilical cord stem cells extraction element, including destroyer and extraction vessel, it is characterised in that：The destroyer includes shell,
Shell one end is connect with the extraction vessel, and shell cover is arranged in the other end, and the shell cover is equipped with through-hole, and shell cover is set towards shell face
There are multiple columnar projections；Further include drawing device, the drawing device includes pulling part, lifting rod and broken portion, and lifting rod passes through institute
Through-hole is stated, broken net is installed in broken portion.
2. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：Under the upper opening of the shell is more than
Opening.
3. umbilical cord stem cells extraction element according to claim 2, it is characterised in that：The shell and extraction vessel are removable
Connection is unloaded, is detachably connected between shell and shell cover.
4. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：The extraction vessel is polytetrafluoroethyl-ne
Alkene material.
5. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：Columnar projections on the inside of the shell cover
Line space for rectangular array, the columnar projections is identical as column pitch.
6. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：The broken portion is square or square
Shape frame, quadrangle are respectively equipped with the lifting rod vertical with frame, and lifting rod end is fixedly connected with pulling part.
7. umbilical cord stem cells extraction element according to claim 6, it is characterised in that：The shell cover is set there are four through-hole.
8. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：The broken net is square mesh knot
Structure, the broken net meshe and columnar protrusions clearance fit.
9. umbilical cord stem cells extraction element according to claim 8, it is characterised in that：The material of the broken net is high-strength
Spend high modulus fibre.
10. umbilical cord stem cells extraction element according to claim 1, it is characterised in that：The shell side is equipped with fluid injection
Hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721881397.5U CN207738757U (en) | 2017-12-28 | 2017-12-28 | Umbilical cord stem cells extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721881397.5U CN207738757U (en) | 2017-12-28 | 2017-12-28 | Umbilical cord stem cells extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207738757U true CN207738757U (en) | 2018-08-17 |
Family
ID=63120455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721881397.5U Expired - Fee Related CN207738757U (en) | 2017-12-28 | 2017-12-28 | Umbilical cord stem cells extraction element |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207738757U (en) |
-
2017
- 2017-12-28 CN CN201721881397.5U patent/CN207738757U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106178134B (en) | A kind of fat transfer material containing highly concentrated adipose-derived stem cells and extracellular matrix and its pure physical preparation method and application | |
| Sensebé et al. | Good manufacturing practices production of mesenchymal stem/stromal cells | |
| US9453200B2 (en) | Apparatus and methods for cell isolation | |
| CN103266081B (en) | Efficient method for isolating and culturing mesenchymal stem cells from umbilical cord | |
| CN101748096B (en) | Sub totipotential stem cell and preparation method and application thereof | |
| CN104762257B (en) | A kind of method preparing mescenchymal stem cell from umbilical cord | |
| CN105238751A (en) | Umbilical cord tissue mesenchymal stem cell isolated culture method | |
| CN106222134A (en) | The cultural method of effective acquisition fat mesenchymal stem cell | |
| CN101591644A (en) | The preparation of umbilical cord-mesenchymal stem cells (UC-MSCs) used in clinical therapy and storage | |
| CN105200007B (en) | The method that Subaerial blue green algae is extracted from placental fetal surface chorion | |
| CN109735490A (en) | A kind of fat stem cell extracting method | |
| CN109486753A (en) | A kind of fat stem cell extracting method | |
| CN101922048A (en) | Method for constructing public library of umbilical mesenchymal stem cells | |
| CN105518126A (en) | Method for culturing mesenchymal stem cells according to cell size | |
| CN106038598A (en) | Method for preparing human-derived stem cell secretion bioactive factor and lysate | |
| CN106434547A (en) | Method for preparing umbilical cord mesenchymal stem cells | |
| US8852923B2 (en) | Flow-stretch-flexure bioreactor | |
| CN108251362A (en) | A kind of preparation method of decidua mescenchymal stem cell | |
| CN207738757U (en) | Umbilical cord stem cells extraction element | |
| CN104152408A (en) | Preparation method of sub-totipotent stem cell | |
| CN102703380B (en) | Sub-totipotent stem cell, preparation method and application thereof | |
| CN106834232A (en) | Pituitary adenoma cell system and application thereof | |
| CN105838604B (en) | For the culture dish of mature fat cell ceiling culture | |
| CN104388385B (en) | A kind of cultural method of human peripheral mescenchymal stem cell and application | |
| CN106109497A (en) | The stem cell medicine for the treatment of liver cirrhosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180817 Termination date: 20201228 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |