CN207733008U - A kind of new structure PCR amplification instrument heating module - Google Patents
A kind of new structure PCR amplification instrument heating module Download PDFInfo
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- CN207733008U CN207733008U CN201721639097.6U CN201721639097U CN207733008U CN 207733008 U CN207733008 U CN 207733008U CN 201721639097 U CN201721639097 U CN 201721639097U CN 207733008 U CN207733008 U CN 207733008U
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Abstract
The utility model discloses a kind of new structure PCR amplification instrument heating modules, heating module includes heat-conducting block, bottom plate, heating plate, heat-conducting block is made from a material that be thermally conductive, the heat-conducting block includes several test tube hole slots formed therein, test tube hole slot is connected with each other by middle part connecting plate, the waist of each test tube hole slot has temperature sensor, adjacent temperature sensor to be not connected to each other；Heat-conducting block lower part is provided with multiple straight slots, and straight slot separates adjacent test tube hole slot, and wiring board is equipped in straight slot, and temperature sensor is connect with wiring board, and test tube hole trench bottom is connect with bottom plate, and heating plate is equipped in bottom plate lower surface.By adding heat-conducting block lower part to open up straight slot and temperature sensor is arranged in straight slot, the temperature in test tube hole slot can be more accurately measured.
Description
Technical field
The utility model is related to a kind of new structure PCR amplification instrument heating module, thermometric and calibration methods.
Background technology
PCR gene amplification instrument is a kind of temperature device, for providing the simulation gene magnification temperature in each stage, and is increased
Add the functions such as low-temperature storage and fluoroscopic examination, is suitable for human genome project, medical jurisprudence, oncology, tissue and group
The polymerase chain of the clinical diagnosises such as the research fields such as biology, paleontology, zoology, botany and virus, tumour, hereditary disease
React fluoremetry detection.
PCR gene amplification instrument currently on the market has the following disadvantages：
1：User can not detect the temperature of 96 reagent wells, only know the temperature of entire module.
2：Since temperature probe is limited, the temperature uniformity that user is seen is caused to have to a certain extent
Deviation.
Utility model content
In order to solve the problems in the existing technology the utility model, devises a kind of new structure PCR amplification instrument and adds
Thermal modules, structurally simple, economical practicality.
To achieve the above object, the technical solution adopted in the utility model is：
A kind of new structure PCR amplification instrument heating module, including heat-conducting block, bottom plate, heating plate, heat-conducting block is by Heat Conduction Material
It is made, the heat-conducting block includes several test tube hole slots formed therein, and test tube hole slot is mutually interconnected by middle part connecting plate
It connects, the waist of each test tube hole slot has temperature sensor, adjacent temperature sensor to be not connected to each other；Heat-conducting block lower part is provided with
Multiple straight slots, straight slot separate adjacent test tube hole slot, and wiring board is equipped in straight slot, and temperature sensor is connect with wiring board, try
Pore trench bottom is connect with bottom plate, and heating plate is equipped in bottom plate lower surface.By adding heat-conducting block lower part to open up straight slot and incite somebody to action
Temperature sensor is arranged in straight slot, can more accurately measure the temperature in test tube hole slot.
As a kind of preferred embodiment of new structure PCR amplification instrument heating module described in the utility model, described is logical
Slot cross-section presents the big trapezium structure in short and small lower end either other polymorphic structures such as square structure or semicircular structure, all belongs to
Within rights protection scope, the side waist in straight slot is arranged in temperature sensor, closer at a distance from sample to be tested, measures more accurate
Really.
Further, the test tube hole slot is distributed in multiple row, and the test tube hole slot lower part of adjacent column is separated by straight slot, same
One piece of wiring board is set in the straight slot of row, multiple pin hole positions are set on wiring board, each pin hole position is correspondingly arranged a sensor,
The quantity of pin hole position is identical as the quantity of test tube hole slot, and the position of pin hole position is corresponding with test tube hole slot.The one-to-one survey of test tube hole slot
Temperature so that measure more accurate.
Preferably, heat insulation structural is additionally provided in straight slot, heat insulation structural shape is corresponding with straight slot structure, and wiring board is along straight slot
Side wall is placed, and then heat insulation structural is filled into straight slot, wiring board can be fixed, ensures that temperature sensor is close to lead to
Slot waist makes the temperature of test tube hole slot control and measure more accurately.
Preferably, further include heat-insulating and sealing circle, the heat-insulating and sealing snare is located at wiring board end and vertical with straight slot
It is affixed.Heat-insulating and sealing circle have allow sealing structure, wiring board by perforation, heat-insulating and sealing circle carries out wiring board and bottom plate
Sealing, wiring board are pierced by from heat-insulating and sealing circle, increase heat insulation and preservation effect.
Further, the heat-conducting block is made of aluminum material.
As a kind of preferred embodiment of new structure PCR amplification instrument heating module described in the utility model, the temperature
Degree sensor is digital temperature sensor, by a single data bus while the multiple temperature sensors of carry.With smaller hardware
Expense realizes the function of multi-point temperature measurement.The number of one side single bus carry temperature sensor is more, the microcontroller of occupancy
Io resources are fewer, the excessive temperature sensor of another aspect single bus carry can cause too high bus load, CPU addressing and
The time cycle of read-write is long, influences efficiency.Preferably, 8 temperature sensors of single data bus carry.
8 temperature sensors hung on the bus are read with one io mouthfuls, after all temperature sensors are parallel with one another
Its data line is connected in some I/O port lines of microprocessor, is first started all temperature sensors and is carried out temperature transition simultaneously
It is shown on liquid crystal display, retransmits reading ROM orders and all temperature sensors of the bus are read and stored, at this time one piece of wiring board
On 8 temperature sensors and liquid crystal display 8 temperature value positions of correspondence vertical setting of types for showing do not correspond to, but random entanglement,
Then soldering iron heating is carried out to first temperature sensor of the wiring board and certain sequence number temperature is detected by the program of setting
The temperature value of sensor is spent in change dramatically, and first display area that vertical setting of types is corresponded on liquid crystal display is assigned to the temperature of permutation row number
Sensor is spent, to complete the position correction of temperature sensor.
Advantageous effect：
New structure PCR amplification instrument heating module described herein, is improved heat-conducting block, and test tube hole slot is one-to-one
Top milling sky is not connected to by thermometric, multizone heating module, and multiple straight slots are formed at lower part, and increase temperature sensing in straight slot
On the one hand device improves heat transfer property, while improving temperature measurement performance.Using multiple heating plates, realize that region is individually controlled
System.
Quantization mean value computation is carried out by real-time collected temperature more accurately to control into trip temperature, it is so very big that reduce
Probe less, coverage area it is small caused by the error come of thermometric and temperature-controlling zone；Facilitate each point in user's real time inspection reaction process
Temperature variations.
Description of the drawings
Fig. 1 is a kind of preferable heating module schematic cross-section.
Fig. 2 is the front schematic view of heating module shown in Fig. 1.
Fig. 3 is the AA schematic cross-sections in Fig. 2.
Fig. 4 is a kind of preferable heating module schematic rear view.
Wherein：01, heat-conducting block 1.1, test tube hole slot 1.2, straight slot 02, wiring board 2.1, temperature sensor 03, filling
Structure 04, bottom plate 05, heating plate 06, heat-insulating and sealing circle
Specific implementation mode
The utility model is described in further detail with reference to the accompanying drawings and detailed description.
Embodiment 1
As shown in Figs 1-4, it is a kind of new structure PCR amplification instrument heating module, heating module includes heat-conducting block 01, bottom plate
04, there is 12*8 to amount to 96 test tube hole slots 1.1 for heating plate 05, wiring board 02 and interstitital texture 03, heat-conducting block 01.
Test tube hole slot 1.1 is connected with each other by middle part connecting plate, and the top milling of each test tube hole slot is empty, can keep in this way
The temperature of the test tube hole slot is preferably preserved, while temperature shadow of the temperature to this test tube hole slot of adjacent test tube hole slot can be reduced
It rings, temperature control is more accurate.
Every piece of wiring board 02 is made of the connector of 8 temperature sensors 2.1 and a 3P, and temperature sensor 2.1 uses
In the circuit board, and the spacing for the spacing and the test tube hole slot on 96 hole heat-conducting blocks arranged is complete for a kind of welded encapsulation of chip type
Unanimously, by wiring board plug to the two rows of gap location of heat-conducting block bottom straight slot 1.2, temperature sensor 2.1 is adjacent to straight slot side on one side
Side designs an interstitital texture 03, just fills up the remaining space in the gap of test tube hole trench bottom two rows.
Heat-conducting block 1 is made from a material that be thermally conductive, and is preferably made of metal thermal conductive material, such as aluminium, obtains required use
Performance that is, constantly in heating temperature-fall period, or in heating down cycles, transmits temperature i.e. adjusted temperature and required temperature soon
Degree be while.
1.1 bottom of test tube hole slot is connect with bottom plate 04, and heating plate 05 is equipped in 04 lower surface of bottom plate.
The big trapezium structure in short and small lower end is presented in 1.2 section of straight slot, can also be square structure, semicircular structure or other
The side waist in through-hole slot structure is arranged in polymorphic structure, temperature sensor 2.1.
1.1 bottom of test tube hole slot flushes, and is connected by bottom plate 04, and heat-conducting block is divided into several heating zones by bottom plate 04
Domain, each bottom plate 04 have heating plate 05, different 05 independent controls of heating plate.
At least one end of wiring board 02 is arranged with heat-insulating and sealing circle 06, and heat-insulating and sealing circle 06 has formed therein
Perforation, can allow wiring board 02 to pass through, while can add sealing structure in junction, and heat-insulating and sealing circle is by wiring board and bottom
Plate is sealed, and wiring board is pierced by from heat-insulating and sealing circle, increases heat insulation and preservation effect.
What temperature sensor 2.1 was selected is a digital temperature sensor, and strong antijamming capability can use a single data
The multiple temperature sensors of carry, the smaller hardware spending of use realize the function of multi-point temperature measurement to bus simultaneously.One side single bus
The number of carry temperature sensor is more, and the io resources of the microcontroller of occupancy are fewer, and another aspect single bus carry is excessive
Temperature sensor can cause too high bus load, CPU addressing and read-write time cycle it is long, influence efficiency.To sum up this production
Product find the best results of single data bus 8 temperature sensors of carry by repeatedly test.
When hanging with multiple temperature sensors on data/address bus, number and each device of the system to device on data/address bus
The Search ROM order for being identified by temperature sensor and algorithm realize.
The present embodiment reads 8 temperature sensors hung on the data/address bus, all temperature sensing with one io mouthfuls
Device it is parallel with one another after its data/address bus be connected in some I/O port lines of microprocessor, outstanding feature be only take up it is micro-
The a port of processor.The key of problem is how to correspond the temperature read and temperature sensor, due to each temperature
Spending sensor internal, there are one unique 64 bit sequence numbers, first can pass host and temperature before system is installed and is worked
Sensor mounts one by one, reads its sequence number respectively and is stored in the FLASH of host, microprocessor can be right according to sequence number
Branched temperature sensor in same bus is identified and controls, and reads their temperature respectively.But this mode has
A drawback：Each temperature sensor will be independently accessed system and read its sequence number and record one by one, this is criticizing product
Big inconvenience can be caused in amount production.
Therefore, we use a kind of completely new calibrating mode, first start all temperature sensors and carry out temperature transition simultaneously
It is shown on liquid crystal display, retransmits reading ROM orders and all temperature sensors of the bus are read and stored, at this time one piece of wiring board
On 8 temperature sensors and liquid crystal display 8 temperature value positions of correspondence vertical setting of types for showing do not correspond to, but random entanglement,
Then soldering iron heating is carried out to first temperature sensor of the wiring board and certain sequence number temperature is detected by the program of setting
The temperature value of sensor is spent in change dramatically, and first display area that vertical setting of types is corresponded on liquid crystal display is assigned to the temperature of permutation row number
Spend sensor.The remaining temperature sensor of the wiring board is corresponded to the display area of liquid crystal display in the method, is completed
Temperature sensor is calibrated.
Although the embodiment of the utility model is illustrated in specification, these embodiments are intended only as carrying
Show, should not limit the scope of protection of the utility model.Various omissions are carried out in the range of not departing from the utility model aims, are set
It changes and change should be included in the scope of protection of the utility model.
Claims (8)
1. a kind of new structure PCR amplification instrument heating module, it is characterised in that：Including heat-conducting block, bottom plate, heating plate, heat-conducting block
It is made from a material that be thermally conductive, the heat-conducting block includes several test tube hole slots formed therein, and test tube hole slot relies on middle part to connect
Fishplate bar is connected with each other, and the waist of each test tube hole slot has temperature sensor, adjacent temperature sensor to be not connected to each other；Heat conduction
Block lower part is provided with multiple straight slots, and straight slot separates adjacent test tube hole slot, and wiring board, temperature sensor and circuit are equipped in straight slot
Plate connects, and test tube hole trench bottom is connect with bottom plate, and heating plate is equipped in bottom plate lower surface.
2. new structure PCR amplification instrument heating module according to claim 1, it is characterised in that：The straight slot section
The big trapezium structure in short and small lower end is presented, the side waist in trapezium structure is arranged in temperature sensor.
3. new structure PCR amplification instrument heating module according to claim 2, it is characterised in that：The test tube hole slot
It is distributed in multiple row, the test tube hole slot lower part of adjacent column is separated by straight slot, and one piece of wiring board, wiring board are arranged in the straight slot of same row
The upper multiple pin hole positions of setting, each pin hole position is correspondingly arranged a sensor, the quantity of pin hole position and the quantity phase of test tube hole slot
Together, the position of pin hole position is corresponding with test tube hole slot.
4. new structure PCR amplification instrument heating module according to claim 3, it is characterised in that：The bottom plate has more
Block, multiple bottom plates are separated from each other and are arranged is correspondingly arranged on heating plate on test tube hole trench bottom, every piece of bottom plate.
5. new structure PCR amplification instrument heating module according to claim 3, it is characterised in that：In the straight slot also
It is provided with interstitital texture.
6. new structure PCR amplification instrument heating module according to claim 3, it is characterised in that：The heat-conducting block by
Aluminum material is made.
7. new structure PCR amplification instrument heating module according to claim 5, it is characterised in that：It further include heat-insulating and sealing
Circle, the heat-insulating and sealing snare are located at wiring board end and vertical with straight slot are affixed.
8. new structure PCR amplification instrument heating module according to claim 3, it is characterised in that：The temperature sensing
Device is digital temperature sensor, by a single data bus while the multiple temperature sensors of carry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721639097.6U CN207733008U (en) | 2017-11-30 | 2017-11-30 | A kind of new structure PCR amplification instrument heating module |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721639097.6U CN207733008U (en) | 2017-11-30 | 2017-11-30 | A kind of new structure PCR amplification instrument heating module |
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| Publication Number | Publication Date |
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| CN207733008U true CN207733008U (en) | 2018-08-14 |
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ID=63089173
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| CN201721639097.6U Active CN207733008U (en) | 2017-11-30 | 2017-11-30 | A kind of new structure PCR amplification instrument heating module |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107949082A (en) * | 2017-11-30 | 2018-04-20 | 南通成时医疗科技有限公司 | A kind of new structure PCR amplification instrument heating module, thermometric and calibration method |
-
2017
- 2017-11-30 CN CN201721639097.6U patent/CN207733008U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107949082A (en) * | 2017-11-30 | 2018-04-20 | 南通成时医疗科技有限公司 | A kind of new structure PCR amplification instrument heating module, thermometric and calibration method |
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