CN207699599U - A kind of automatic filling device for cell culture medium - Google Patents

Abstract

The utility model discloses a kind of automatic filling devices for cell culture medium, including container, the bottom end of the container is equipped with multiple supporting racks, the upper end of the container is communicated with feed pipe, check-valves is installed on the feed pipe, the lower end of the container is communicated with outlet pipe, it is fixedly connected with fixed frame on the outer wall of the container, the outlet pipe is fixed on by fixed frame on the outer wall of container, the one end of the outlet pipe far from container is communicated with nozzle, the upper end of the container is installed by organism, the body and reservoir, the upper end of the container is fixedly connected with holder, the upper end of the holder is connected with decelerating motor, the output sleeve of the decelerating motor is equipped with wheel disc, the eccentric part of the wheel disc is rotatably connected to connecting rod.The utility model stable structure, easy to operate, design is scientific and reasonable, and piston can be pushed to carry out feeding filling automatically, a large amount of time and efforts of staff can be saved, be worthy to be popularized.

Description

A kind of automatic filling device for cell culture medium

Technical field

The utility model is related to cell culture medium filling apparatus technical fields more particularly to a kind of for cell culture medium Automatic filling device.

Background technology

Since 21st century, important function of the cell therapy in clinical treatment is increasingly bright and clear, especially Stem cell, because it is with self-renewing, multi-lineage potential be more considered as ideal kind of cell therapy and organizational project it is careful Born of the same parents.Such as mesenchymal stem cell and umbilical cord mesenchymal stem cells can be used for treating autoimmune disease and regenerative medicine, However, source of human stem cell is limited, culture is complicated for operation, and growing environment, which varies slightly, will influence culture effect, and stem cell Application be to be transfused according to the kg body weight of patient, need a large amount of amplification cultivation to can be only achieved quantitative requirement.In cell In the practical application of culture, it is a daily sex work to fill culture medium respectively for a large amount of Tissue Culture Flasks, due to not having also now There is the higher adding set of automation so that the filling of culture medium becomes a hard work.Although needle-based may be used Filter, which fills culture medium, reduces opportunities for contamination, but in mass propgation cell, and the hand of operator can be made by pushing and pulling syringe bolt repeatedly Portion is excessively tired, it is therefore desirable to design a kind of automatic filling device.

Utility model content

Purpose of the utility model is to solve push-and-pull syringe bolt can make the hand of operator repeatedly in the prior art Excessively tired problem, and a kind of automatic filling device for cell culture medium proposed.

To achieve the goals above, the utility model uses following technical solution：

The bottom end of a kind of automatic filling device for cell culture medium, including container, the container is equipped with multiple The upper end of support, the container is communicated with feed pipe, and check-valves is equipped on the feed pipe, and the lower end of the container is communicated with Outlet pipe is fixedly connected with fixed frame on the outer wall of the container, and the outlet pipe is fixed on the outer wall of container by fixed frame On, the one end of the outlet pipe far from container is communicated with nozzle, and organism, the body and container are installed in the upper end of the container Connection, the upper end of the container are fixedly connected with holder, and the upper end of the holder is connected with decelerating motor, the decelerating motor Output sleeve is equipped with wheel disc, and the eccentric part of the wheel disc is rotatably connected to connecting rod, and one end rotation of the connecting rod far from wheel disc connects It is connected to piston rod, the lower end of the piston rod is fixedly connected with piston, and sealing structure is used between piston and the inner wall of body.

Preferably, the holder uses door type structure.

Preferably, the decelerating motor uses planet-gear speed reducing motor.

Preferably, the output shaft of the decelerating motor is coupled by flat key with wheel disc interference fit.

Preferably, the lower end of the piston is fixedly connected with the body of rod, and the one end of the body of rod far from piston is connected with the second work Plug.

Preferably, the height of the nozzle is higher than the height of container.

In the utility model, in use, opening decelerating motor, the output shaft of decelerating motor drives the wheel of connection fixed thereto Disk rotates, and rotationally connected link motion is driven after wheel disc rotation, and connecting rod drives piston rod elevating movement, after piston motion The gas compression for driving container, to make the solution of container be sprayed from nozzle via outlet pipe, since the height of nozzle is higher than appearance Device, this can highly be adjusted according to actual conditions, therefore the liquid of container will not be sprayed from nozzle under normality, and due on water inlet pipe Check-valves is installed, therefore when piston upwards, air inlet in container can be made, is filled repeatedly to realize.The utility model knot Structure is stablized, and easy to operate, design is scientific and reasonable, and piston can be pushed to carry out feeding filling automatically, it is a large amount of can to save staff Time and efforts is worthy to be popularized.

Description of the drawings

Fig. 1 be the utility model proposes a kind of automatic filling device for cell culture medium structural schematic diagram；

Fig. 2 be the utility model proposes a kind of automatic filling device for cell culture medium sectional view.

In figure：1 supporting rack, 2 containers, 3 bodies, 4 holders, 5 wheel discs, 6 outlet pipes, 7 nozzles, 8 connecting rods, 9 piston rods, 10 are lived Plug, 11 bodies of rod, 12 second pistons, 13 feed pipes, 14 check-valves, 15 fixed frames.

Specific implementation mode

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.

In the description of the present invention, it should be understood that term "upper", "lower", "front", "rear", "left", "right", The orientation or positional relationship of the instructions such as "top", "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only The utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specific Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.

Referring to Fig.1-2, a kind of automatic filling device for cell culture medium, including container 2, the bottom end installation of container 2 There are multiple supporting racks 1, the upper end of container 2 to be communicated with feed pipe 13, check-valves 14, the lower end of container 2 are installed on feed pipe 13 It is communicated with outlet pipe 6, fixed frame 15 is fixedly connected on the outer wall of container 2, outlet pipe 6 is fixed on container 2 by fixed frame 15 Outer wall on, the one end of outlet pipe 6 far from container 2 is communicated with nozzle 7, and the height of nozzle 7 is higher than the height of container 2, according to height Water level flows to the separate of low water level, and organism 3 is installed in the upper end of container 2, and body 3 is connected to container 2；

In the utility model, the upper end of container 2 is fixedly connected with holder 4, and holder 4 uses door type structure, holder 4 it is upper End is connected with decelerating motor, and decelerating motor uses planet-gear speed reducing motor, and planet-gear speed reducing motor is small, and rotating speed is slow, Torque is big, and the output sleeve of decelerating motor is equipped with wheel disc 5, and the output shaft of decelerating motor is joined by flat key and the interference fit of wheel disc 5 It connects, interference fit connecting process is simple, and the eccentric part of wheel disc 5 is rotatably connected to connecting rod 8, one end rotation of the connecting rod 8 far from wheel disc 5 It is connected with piston rod 9, the lower end of piston rod 9 is fixedly connected with piston 10, and the lower end of piston 10 is fixedly connected with the body of rod 11, and bar The one end of body 11 far from piston 10 is connected with second piston 12, and the setting of second piston 12 is convenient for further increasing sealing performance, And sealing structure is used between piston 10 and the inner wall of body 3.

The electric elements occurred in this article are electrically connected with extraneous main controller and 220V alternating currents, and main controller can be meter Calculation machine etc. plays the conventionally known equipment of control.

Piston 10 and piston rod 9 are the prior art in the utility model, and details are not described herein again, what the utility model solved Technical problem is：Push-and-pull syringe bolt can make the hand of operator excessively tired repeatedly.

When the utility model is used, decelerating motor is opened, the output shaft of decelerating motor drives the wheel disc 5 of connection fixed thereto Rotation, wheel disc 5 drive rotationally connected connecting rod 8 to move after rotating, and connecting rod 8 drives 9 elevating movement of piston rod, piston 10 to transport The gas compression that container 2 is driven after dynamic, to make the solution of container 2 be sprayed from nozzle 7 via outlet pipe 6, due to the height of nozzle 7 Degree is higher than container 2, this can highly be adjusted according to actual conditions, therefore the liquid of container 2 will not be sprayed from nozzle 7 under normality, and Due to being equipped with check-valves 14 on water inlet pipe 6, when piston 10 moves upwards, air inlet in container 2 can be made, to realize repeatedly Filling.

The preferable specific implementation mode of the above, only the utility model, but the scope of protection of the utility model is not Be confined to this, any one skilled in the art within the technical scope disclosed by the utility model, according to this practicality Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model Within enclosing.

Claims (6)

1. a kind of automatic filling device for cell culture medium, including container (2), which is characterized in that the bottom of the container (2) End is equipped with multiple supporting racks (1), and the upper end of the container (2) is communicated with feed pipe (13), is installed on the feed pipe (13) There are check-valves (14), the lower end of the container (2) to be communicated with outlet pipe (6), be fixedly connected on the outer wall of the container (2) Determine frame (15), the outlet pipe (6) is fixed on by fixed frame (15) on the outer wall of container (2), and the outlet pipe (6) is far from appearance One end of device (2) is communicated with nozzle (7), the upper end installation organism (3) of the container (2), the body (3) and container (2) Connection, the upper end of the container (2) are fixedly connected with holder (4), and the upper end of the holder (4) is connected with decelerating motor, described The output sleeve of decelerating motor is equipped with wheel disc (5), and the eccentric part of the wheel disc (5) is rotatably connected to connecting rod (8), the connecting rod (8) one end far from wheel disc (5) is rotatably connected to piston rod (9), and the lower end of the piston rod (9) is fixedly connected with piston (10), sealing structure and between piston (10) and the inner wall of body (3) is used.

2. a kind of automatic filling device for cell culture medium according to claim 1, which is characterized in that the holder (4) door type structure is used.

3. a kind of automatic filling device for cell culture medium according to claim 1, which is characterized in that the deceleration Motor uses planet-gear speed reducing motor.

4. a kind of automatic filling device for cell culture medium according to claim 1, which is characterized in that the deceleration The output shaft of motor is coupled by flat key with wheel disc (5) interference fit.

5. a kind of automatic filling device for cell culture medium according to claim 1, which is characterized in that the piston (10) lower end is fixedly connected with the body of rod (11), and the one end of the body of rod (11) far from piston (10) is connected with second piston (12).

6. a kind of automatic filling device for cell culture medium according to claim 1, which is characterized in that the nozzle (7) height is higher than the height of container (2).