CN207571030U - A kind of specimen holder for being used to measure solid and liquid surface fluorescence - Google Patents
A kind of specimen holder for being used to measure solid and liquid surface fluorescence Download PDFInfo
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- CN207571030U CN207571030U CN201721710418.7U CN201721710418U CN207571030U CN 207571030 U CN207571030 U CN 207571030U CN 201721710418 U CN201721710418 U CN 201721710418U CN 207571030 U CN207571030 U CN 207571030U
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- Prior art keywords
- solid
- specimen holder
- pond
- cell
- backup plate
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- 239000007787 solid Substances 0.000 title claims abstract description 63
- 239000007788 liquid Substances 0.000 title claims abstract description 44
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229920003266 Leaf® Polymers 0.000 claims description 20
- 239000010453 quartz Substances 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 230000005284 excitation Effects 0.000 claims description 7
- 239000004677 Nylon Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000001917 fluorescence detection Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000013461 design Methods 0.000 description 4
- 238000000695 excitation spectrum Methods 0.000 description 4
- 238000002189 fluorescence spectrum Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000004445 quantitative analysis Methods 0.000 description 3
- 238000000295 emission spectrum Methods 0.000 description 2
- 230000005283 ground state Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 235000005811 Viola adunca Nutrition 0.000 description 1
- 235000013487 Viola odorata Nutrition 0.000 description 1
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- 238000002284 excitation--emission spectrum Methods 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
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Abstract
The utility model is related to technical field of fluorescence detection, specifically a kind of specimen holder for being used to measure solid and liquid surface fluorescence, including specimen holder ontology, solid pond, liquid cell, specimen holder ontology includes pedestal, shading bent plate, taper liner block, backup plate, spring leaf, the bottom of shading bent plate is fixed on using screw on pedestal, taper liner block is fixed on the middle of the base using pin and screw, backup plate is fixed on the surrounding of taper liner block, backup plate forms hollow column structure, solid pond or liquid cell are inserted into column structure, and are fastened using spring leaf one.The utility model is compared with the existing technology, solid sample is positioned in solid pond, facilitate fixation, solid pond can very easily be put into specimen holder, and similar cuvette is put into cell rack so simple, multiple solid ponds can be equipped with, facilitate the mutual contrastive test of various samples, while be equipped with liquid cell, for completing liquid surface fluoremetry, it is simple and convenient without removing or adjusting pedestal shelf.
Description
Technical field
The utility model is related to technical field of fluorescence detection, and specifically one kind is used to measure solid and liquid surface is glimmering
The specimen holder of light.
Background technology
Sepectrophotofluorometer is a kind of instrument of fluorescence spectrum that test fluorescent material can be used for be sent out.It can be provided
Including many physical parameters such as excitation spectrum, emission spectrum and fluorescence intensity.It, not only can be with by the measure to these parameters
Do general quantitative analysis, and may also be inferred that the conformation change of molecule under circumstances, so as to illustrate molecular structure with
Relationship between function.
The excitation wavelength scanning range of sepectrophotofluorometer is usually 200~900nm, and launch wavelength scanning range is
200~900nm.It is generally used for the spectral measurement of liquid.
The basic principle of sepectrophotofluorometer is the ultraviolet light and blue violet light sent out by high-pressure sodium lamp or xenon lamp through monochrome
Device light splitting is irradiated in sample cell, the fluorescent material in sample is excited to send out fluorescence, fluorescence is after transmitting monochromator light splitting, quilt
Photomultiplier is received, and is then shown in the form of scheming or is digital.
The generation of substance fluorescence is to become exciting from being under normal conditions after the material molecule of ground state absorbs exciting light
State, these molecules for being in excitation state are unstable, by the energy of a part again with the shape of light during ground state is returned
Formula is released, so as to generate fluorescence.
For different material due to the difference of molecular structure, the distribution of excited level has respectively different features, this
Feature, which is reflected on fluorescence, to be shown as various substances and has its characteristic fluorescence excitation and emission spectra, therefore can use fluorescence excitation
The identification of substance is qualitatively carried out with the difference of emission spectrum.
In the solution, when the concentration of fluorescent material is relatively low, the concentration of fluorescence intensity and the substance usually has good
Proportional relation, i.e. IF=KC can carry out the quantitative analysis of fluorescent material using this relationship, with spectrophotometry
Method is similar, and fluorescence analysis is generally also carried out using calibration curve method.
At present, the sepectrophotofluorometer major design of mainstream carrys out test liquid sample in the market, provisioned in equipment all
It is liquid sample stand.The fluorometric investigation of fluid sample is also the fluorescence signal inside main test liquid, when liquid fluorescence itself
When material concentration is too high or liquid also has other lighttight interfering substances in itself, exciting light cannot penetrate liquid surface
Enter liquid internal position, can cause fluorescent liquid test occur fluorescent quenching or it is other similar the phenomenon that, can not complete
Fluorescent liquid quantifies or qualitative test.
For this kind of fluid sample, can fluoroscopic examination be carried out by the position to fluid sample front surface, realized to fluorescence
The quantitative and qualitative analysis of substance.In actual use, it if necessary to the fluorescence of measurement solid sample, can also be equipped with corresponding
Solid sample holder, to realize the fluorescence measurement of solid sample.But it is required to during using solid sample holder by original liquid
Specimen holder is removed.It makes troubles to user.
Therefore, it is necessary to design a kind of while compatible solid and the fluorimetric specimen holder of liquid surface.
Invention content
The purpose of the utility model is to overcome the deficiencies in the prior art, provide a kind of while compatible solid and liquid surface
Fluorimetric specimen holder.
In order to achieve the above object, the utility model is a kind of for measuring the specimen holder of solid and liquid surface fluorescence,
Including specimen holder ontology, solid pond, liquid cell, it is characterised in that：Specimen holder ontology includes pedestal, shading bent plate, taper liner block, leans on
Plate one, backup plate two, spring leaf one, the bottom of shading bent plate are fixed on using screw on pedestal, and shading bent plate is by two plane bends
It rolls over, a plane is equipped with excitation light window, another plane is equipped with transmitting light window, and taper liner block uses pin and spiral shell
Nail is fixed on the middle of the base, and backup plate one, backup plate two are fixed on the surrounding of taper liner block using screw, and spring leaf one, which is adopted, to be screwed
On the both sides top of backup plate two, backup plate one, backup plate two form hollow column structure, and solid pond or liquid cell are inserted into column structure
It is interior, and fastened using spring leaf one.
The solid pond includes baffle, solid sample pond, briquetting, quartz plate, knob, spring, locating part, coiled hair spiral shell
Nail, baffle are adhered on solid sample pond, and baffle is equipped with oblique angle structure one, and pan head screw is fixed in solid sample pond, disk
It after the end of head screw passes through the side of briquetting and fixes, is connect with knob, spring, briquetting is equipped between pan head screw and briquetting
Opposite side be equipped with oblique angle structure two, quartz plate is stuck between oblique angle structure one and oblique angle structure two, the side in solid sample pond
Equipped with sample cell, quartz plate covers sample cell, and the top in solid sample pond is equipped with locating part.
The briquetting is nylon briquetting.
The liquid cell includes cell rack, cuvette, spring leaf two, and two spring leafs two are mounted on cell rack phase
The top of adjacent both sides, cuvette are inserted into cell rack column structure, and are fastened using spring leaf two.
Compared with the prior art, the utility model designs compatible solid and the fluorimetric samples of liquid surface simultaneously
Frame, solid sample are positioned in solid pond, facilitate fixation, and solid pond can very easily be put into specimen holder, and similar cuvette is put
Enter in cell rack so simply, multiple solid ponds can be equipped with, facilitate the mutual contrastive test of various samples, while be equipped with liquid
Pond, it is simple and convenient without removing or adjusting pedestal shelf for completing liquid surface fluoremetry.The utility model application
In sepectrophotofluorometer.
Description of the drawings
Fig. 1 is the specimen holder structure diagram of the utility model.
Fig. 2 is the specimen holder sectional view of the utility model.
Fig. 3 is the solid pool structure schematic diagram of the utility model.
Fig. 4 is the solid pond sectional view of the utility model.
Fig. 5 is the liquid cell structure diagram of the utility model.
Fig. 6 is the liquid cell vertical view of the utility model.
Specific embodiment
The utility model is described further in conjunction with attached drawing.
Referring to Fig. 1-2, the utility model is a kind of for measuring the specimen holder of solid and liquid surface fluorescence, including sample
Frame ontology, solid pond, liquid cell, specimen holder ontology 1 include pedestal 1.7, shading bent plate 1.5, taper liner block 1.6, backup plate 1,
Backup plate 2 1.3, spring leaf 1, the bottom of shading bent plate 1.5 are fixed on using screw on pedestal 1.7, shading bent plate 1.5 by
Two plane bendings form, and a plane is equipped with excitation light window 1.5.1, another plane is equipped with transmitting light window
1.5.2, other direction light barreds reduce stray light.Taper liner block 1.6 is fixed on using pin 1.1 and screw in pedestal 1.7
Portion, taper liner block 1.6 can rotate, and slewing area is 30 °.Backup plate 1, backup plate 2 1.3 are fixed on taper liner block 1.6 using screw
Surrounding, have certain angle with pedestal 1.7, effectively avoid interference of the scattering of excitation spectrum to fluorescence spectrum signal.Spring leaf
One 1.4 are fixed on the both sides top of backup plate 2 1.3 using screw, ensure solid pond 2 and liquid cell 3 plug accurate positioning every time.
Backup plate 1, backup plate 2 1.3 form hollow column structure, and specimen holder ontology 1 is universal base, solid pond 2 or liquid cell
3 are inserted into column structure, and are fastened using spring leaf 1.
Referring to Fig. 3-4, solid pond 2 include baffle 2.3, solid sample pond 2.2, briquetting 2.4, quartz plate 2.1, knob 2.6,
Spring 2.5, locating part 2.7, pan head screw 12, baffle 2.3 are adhered on solid sample pond 2.2, and baffle 2.3 is equipped with oblique angle knot
Structure one, pan head screw 12 are fixed in solid sample pond 2.2, and the end of pan head screw 12 passes through side and the fixation of briquetting 2.4
Afterwards, it is connect with knob 2.6, spring 2.5 is equipped between pan head screw 12 and briquetting 2.4, the opposite side of briquetting 2.4 is equipped with oblique angle knot
Structure two, quartz plate 2.1 are stuck between oblique angle structure one and oblique angle structure two, and briquetting 2.4 is nylon briquetting, prevents quartz plate 2.1
It being crushed by overexertion, the side in solid sample pond 2.2 is equipped with sample cell 2.2.1, and quartz plate 2.1 covers sample cell 2.2.1, Gu
The top of body sample cell 2.2 is equipped with locating part 2.7.
The position of briquetting 2.4 is adjusted by knob 2.6, allows oblique angle structure one with oblique angle structure two quartz plate 2.1
It is pressed on solid sample pond 2.2, to cover the sample in solid sample slot 2.2.1.When knob 2.6 screws out outward, spring 2.5
Pop up briquetting 2.4, conveniently take out quartz plate 2.1, locating part 2.7, which plays, avoids excessive turn knob 2.6, prevent knob 2.6 because
Excessively rotation and what is fallen happen.
Referring to Fig. 5-6, liquid cell 3 includes cell rack 3.3, cuvette 3.1, spring leaf 2 3.2, two spring leafs two
3.2 are mounted on the top of 3.3 adjacent both sides of cell rack, and cuvette 3.1 is inserted into 3.3 column structure of cell rack, and is used
Spring leaf 2 3.2 fastens, and spring leaf 2 3.2 ensures that 3.1 each insertion position of cuvette is consistent.
The utility model while the measure of compatible solid sample and fluid sample surface fluorescence.Two applications without dismounting and
Pedestal shelf is adjusted, realizes seamless connection.User without replacing test specimens repeatedly because of alternately testing liquid and solid sample
Product frame.
Solid sample is positioned in the solid sample pond 2.2 of exploitation formula, convenient for filling sample, also allows for cleaning and replacing sample.
Solid pond 2 is placed into specimen holder ontology 1, and solid pond 2 is put into specimen holder ontology 1 without auxiliary tools such as screws, directly
Insertion coordinates one 1.4 accurate positioning of spring leaf, easy to use.Solid sample pond and 1 separate design of specimen holder ontology, can
Multiple solid ponds 2 are equipped with, realize solid sample test comparison repeatedly, without repeating dress sample operation.
Fluid sample is put into conventional fluorescent cuvette 3.1, places into liquid cell 3, uses process and conventional fluorescent colorimetric
Ware is the same, and accurate positioning, easily realizes that the surface fluorescence of fluid sample measures.
Specimen holder ontology 1 has certain angle of inclination, and it is flat that the exciting light of sample can be scattered to fluorescence spectrum detection
On face, interference of the scattering of excitation spectrum to fluorescence spectrum signal is effectively avoided.
Claims (4)
- It is 1. special including specimen holder ontology, solid pond, liquid cell kind for measuring the specimen holder of solid and liquid surface fluorescence Sign is：Specimen holder ontology（1）Including pedestal（1.7）, shading bent plate（1.5）, taper liner block（1.6）, backup plate one（1.2）, backup plate Two（1.3）, spring leaf one（1.4）, shading bent plate（1.5）Bottom pedestal is fixed on using screw（1.7）On, shading bent plate （1.5）It is formed by two plane bendings, a plane is equipped with excitation light window（1.5.1）, another plane, which is equipped with, to be emitted Light window（1.5.2）, taper liner block（1.6）Using pin（1.1）Pedestal is fixed on screw（1.7）Middle part, backup plate one（1.2）、 Backup plate two（1.3）Taper liner block is fixed on using screw（1.6）Surrounding, spring leaf one（1.4）Backup plate two is fixed on using screw （1.3）Both sides top, backup plate one（1.2）, backup plate two（1.3）Form hollow column structure, solid pond（2）Or liquid cell （3）It is inserted into column structure, and uses spring leaf one（1.4）Fastening.
- 2. a kind of specimen holder for being used to measure solid and liquid surface fluorescence according to claim 1, it is characterised in that：Institute The solid pond stated（2）Including baffle（2.3）, solid sample pond（2.2）, briquetting（2.4）, quartz plate（2.1）, knob（2.6）, bullet Spring（2.5）, locating part（2.7）, pan head screw（12）, baffle（2.3）It is adhered to solid sample pond（2.2）On, baffle（2.3）On Equipped with oblique angle structure one, pan head screw（12）It is fixed on solid sample pond（2.2）In, pan head screw（12）End pass through briquetting （2.4）Side and after fixing, with knob（2.6）Connection, pan head screw（12）With briquetting（2.4）Between be equipped with spring（2.5）, Briquetting（2.4）Opposite side be equipped with oblique angle structure two, quartz plate（2.1）It is stuck between oblique angle structure one and oblique angle structure two, Gu Body sample cell（2.2）Side be equipped with sample cell（2.2.1）, quartz plate（2.1）Cover sample cell（2.2.1）, solid sample pond （2.2）Top be equipped with locating part（2.7）.
- 3. a kind of specimen holder for being used to measure solid and liquid surface fluorescence according to claim 2, it is characterised in that：Institute The briquetting stated（2.4）For nylon briquetting.
- 4. a kind of specimen holder for being used to measure solid and liquid surface fluorescence according to claim 1, it is characterised in that：Institute The liquid cell stated（3）Including cell rack（3.3）, cuvette（3.1）, spring leaf two（3.2）, two spring leafs two（3.2）Peace Mounted in cell rack（3.3）The top of adjacent both sides, cuvette（3.1）It is inserted into cell rack（3.3）In column structure, and adopt With spring leaf two（3.2）Fastening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721710418.7U CN207571030U (en) | 2017-12-11 | 2017-12-11 | A kind of specimen holder for being used to measure solid and liquid surface fluorescence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721710418.7U CN207571030U (en) | 2017-12-11 | 2017-12-11 | A kind of specimen holder for being used to measure solid and liquid surface fluorescence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207571030U true CN207571030U (en) | 2018-07-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721710418.7U Expired - Fee Related CN207571030U (en) | 2017-12-11 | 2017-12-11 | A kind of specimen holder for being used to measure solid and liquid surface fluorescence |
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| Country | Link |
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| CN (1) | CN207571030U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021098547A1 (en) * | 2019-11-20 | 2021-05-27 | 华南理工大学 | Synchronous fluorescence detection device having fluorescent contaminant interfacial concentration and use method therefor |
-
2017
- 2017-12-11 CN CN201721710418.7U patent/CN207571030U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021098547A1 (en) * | 2019-11-20 | 2021-05-27 | 华南理工大学 | Synchronous fluorescence detection device having fluorescent contaminant interfacial concentration and use method therefor |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180703 |
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| CF01 | Termination of patent right due to non-payment of annual fee |