CN207516633U - Glass slide - Google Patents
Glass slide Download PDFInfo
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- CN207516633U CN207516633U CN201720931360.2U CN201720931360U CN207516633U CN 207516633 U CN207516633 U CN 207516633U CN 201720931360 U CN201720931360 U CN 201720931360U CN 207516633 U CN207516633 U CN 207516633U
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- China
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- shrinkage pool
- glass slide
- region
- coverslip
- glossy clear
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a kind of glass slide, including：Glossy clear region and frosting region, the glossy clear region are located at frosting region side, and the glossy clear regional center is equipped with shrinkage pool.The utility model carries glossy clear region and is equipped with shrinkage pool, and cell liquid is added dropwise at shrinkage pool so that cell compartment easily identifies, easy to operation when adding probe mixture and DAPI (4,6 two miaow base, 2 xenyl indoles).Coverslip is caught in shrinkage pool simultaneously, and coverslip will not be with movement when 100 × oil mirror or so observes fluorescence signal.The glass slide prepared is inserted into slide box when 20 DEG C of refrigerators preserve, and coverslip is without departing from origin-location.Region is added dropwise to fix, convenient for Quality Control.
Description
Technical field
The utility model is related to biological culture pipe manufacturing fields, and in particular to a kind of glass slide.
Background technology
Fluorescence in situ hybridization (fluorescence in situ hybridization, FISH) is in the 1980s
A kind of on-radiation molecular cytogenetics technology that end grows up on the basis of radioactive in situ hybridization technology, with fluorescence mark
A kind of new in-situ hybridization method kept in mind for isotope labelling and formed, probe first with certain reporter molecule (reporter
Molecule) combine, after hybridization again by immunocytochemical procedures connect upper fluorescent dye .FISH basic principle be by
DNA (or RNA) probes are marked with special nucleic acid molecule, are then sliced probe direct cross to chromosome or DNA fiber
On, then specifically bound with probe molecule with the monoclonal antibody that is coupled with fluorescein molecule to detect DNA sequence dna in chromosome
Or qualitative, positioning on DNA fiber slice, relative quantitative assay .FISH have safety, quick, high sensitivity, probe can be long-term
It preserves, can show the advantages that multiple color simultaneously, can not only show division phases, moreover it is possible to be shown in interphase nucleus simultaneously glimmering
Multicolor FISH technology and chromatin fiber fluorescence in situ hybridization technique are developed again on the basis of light in situ hybridization.
Glass slide is to be rectangle in above-mentioned experiment for placing the sheet glass of experiment material, thicker, have it is preferable thoroughly
Photosensitiveness.The shortcomings that common glass slide：1) since fluorescence in situ hybridization experiment operates in darkroom, cell compartment naked eyes are not easy to know
Not, to probe and DAPI is added to bring difficulty.2) since mirror oil has certain viscosity, when 100 × oil mirror observes cell fluorescence signal,
It is easy to stick together with coverslip during moving left and right, leads to the original position change of coverslip, influence the interpretation of signal.3)
The glass slide prepared is inserted into slide box when -20 DEG C of refrigerators preserve, and coverslip is readily moved to wave carrier piece one with mirror oil
Side.So that when requirement of experiment observes this glass slide again, need to take off mirror oil, be capped new coverslip again, it is time-consuming
Arduously.4) cell suspension is dripped to that the position on glass slide is different by different technicians, leads to the glass slide finally prepared
Disunity is not easy to Quality Control.
Utility model content
The utility model is in view of the above-mentioned problems, propose a kind of glass slide, the detection zone for solving existing glass slide
The defects of being not fixed, be difficult to observe, easily sliding.
The technical solution that the utility model is taken is as follows：
A kind of glass slide, including：Glossy clear region and frosting region, the glossy clear region are located at frosting area
Domain side, the glossy clear regional center are equipped with shrinkage pool.The utility model is equipped with shrinkage pool in glossy clear region, at shrinkage pool
Cell liquid is added dropwise so that cell compartment easily identifies, when adding probe mixture and DAPI (4,6- bis- miaow base -2- xenyls indoles)
It is easy to operation.Coverslip is caught in shrinkage pool simultaneously, and coverslip will not be with movement when 100 × oil mirror or so observes fluorescence signal.
The glass slide prepared is inserted into slide box when -20 DEG C of refrigerators preserve, and coverslip is without departing from origin-location.Region is added dropwise to consolidate
It is fixed, convenient for Quality Control.
Optionally, the shrinkage pool is circle.
The distance of upper and lower side of the center of circle of the optional shrinkage pool to glass slide is consistent.
The diameter of the optional shrinkage pool is more than the distance of the center of circle to the upper end of glass slide of shrinkage pool.
The depth of the optional shrinkage pool is 0.2mm.
The beneficial effects of the utility model are：The utility model carries glossy clear region and is equipped with shrinkage pool, is added dropwise at shrinkage pool
Cell liquid so that cell compartment easily identifies, and is convenient for when adding probe mixture and DAPI (4,6- bis- miaow base -2- xenyls indoles)
Operation.Coverslip is caught in shrinkage pool simultaneously, and coverslip will not be with movement when 100 × oil mirror or so observes fluorescence signal.It prepares
Good glass slide is inserted into slide box when -20 DEG C of refrigerators preserve, and coverslip is without departing from origin-location.Region is added dropwise to fix, just
In Quality Control.
Description of the drawings：
Fig. 1 is the main structure diagram of the utility model glass slide.
Each reference numeral is in figure：
1st, glossy clear region；2nd, frosting region；3rd, shrinkage pool.
Specific embodiment：
With reference to each attached drawing, the utility model is described in detail.
The utility model discloses a kind of glass slide (see attached drawing 1), including：Glossy clear region 1 and frosting region 2,
The glossy clear region 1 is located at 2 side of frosting region, and 1 center of glossy clear region is equipped with shrinkage pool 3.This practicality is new
Type is equipped with shrinkage pool 3 in glossy clear region 1, cell liquid is added dropwise at shrinkage pool 3 so that cell compartment easily identifies, and probe is added to mix
It closes easy to operation when object and DAPI (4,6- bis- miaow base -2- xenyls indoles).Coverslip is caught in shrinkage pool 3 simultaneously, 100 × oil mirror
Coverslip will not be with movement during the observation fluorescence signal of left and right.The glass slide prepared is inserted into slide box and is preserved in -20 DEG C of refrigerators
When, coverslip is without departing from origin-location.Region is added dropwise to fix, convenient for Quality Control.
The shrinkage pool 3 is circle.The distance of upper and lower side of the center of circle of the shrinkage pool 3 to glass slide is consistent.
The diameter of the shrinkage pool 3 is more than the distance of upper end of the center of circle of shrinkage pool 3 to glass slide.The depth of the shrinkage pool 3 is
0.2mm。
When the utility model is implemented, 20 μ l cell suspensions are drawn with liquid-transfering gun, blood disease fluorescence is highly dropped in about 10cm
In the circular shrinkage hole (glass slide must be bright and clean, and thickness is uniform, without apparent autofluorescence) of in situ hybridization glass slide, and dry in the air in room temperature
It is dry.
The above is only the preferred embodiment of the present invention, not thereby limits the patent protection of the utility model
Range, every equivalent structure transformation made with the utility model specification and accompanying drawing content, is directly or indirectly used in it
His relevant technical field similarly includes within the protection scope of the present utility model.
Claims (4)
1. a kind of glass slide, which is characterized in that including：Glossy clear region and frosting region, the glossy clear region position
In frosting region side, the glossy clear regional center is equipped with shrinkage pool；The depth of the shrinkage pool is 0.2mm.
2. glass slide as described in claim 1, which is characterized in that the shrinkage pool is circle.
3. glass slide as described in claim 1, which is characterized in that the distance of the upper and lower side in the center of circle of the shrinkage pool to glass slide
Unanimously.
4. glass slide as claimed in claim 3, which is characterized in that the diameter of the shrinkage pool is more than the center of circle of shrinkage pool to glass slide
Upper end distance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720931360.2U CN207516633U (en) | 2017-07-28 | 2017-07-28 | Glass slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720931360.2U CN207516633U (en) | 2017-07-28 | 2017-07-28 | Glass slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207516633U true CN207516633U (en) | 2018-06-19 |
Family
ID=62531198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720931360.2U Active CN207516633U (en) | 2017-07-28 | 2017-07-28 | Glass slide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207516633U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111257302A (en) * | 2020-03-20 | 2020-06-09 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
-
2017
- 2017-07-28 CN CN201720931360.2U patent/CN207516633U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111257302A (en) * | 2020-03-20 | 2020-06-09 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
| CN111257302B (en) * | 2020-03-20 | 2021-05-04 | 中国科学院微生物研究所 | Liquid sample pool for detecting aerobic or facultative anaerobic pathogenic microorganisms by micro-Raman spectrum |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 311100 rooms 106 and 203, building 1, No. 181, Wuchang Avenue, Wuchang Street, Yuhang District, Hangzhou, Zhejiang Province Patentee after: Hangzhou qianmai medical laboratory Co.,Ltd. Address before: 311100 building 4, Huazheng science and Technology Park, Jinxing Second Road, Yuhang street, Yuhang District, Hangzhou City, Zhejiang Province Patentee before: HANGZHOU CHAIN MEDICAL LABS CO.,LTD. |