CN207452077U - A kind of gene sequencing chip loading device - Google Patents

A kind of gene sequencing chip loading device Download PDF

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Publication number
CN207452077U
CN207452077U CN201721280542.4U CN201721280542U CN207452077U CN 207452077 U CN207452077 U CN 207452077U CN 201721280542 U CN201721280542 U CN 201721280542U CN 207452077 U CN207452077 U CN 207452077U
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pump
topping
gene sequencing
reagent
sequencing chip
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CN201721280542.4U
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Inventor
张焕贵
梁亚运
罗杵添
倪鸣
魏栋
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MGI Tech Co Ltd
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Shenzhen BGI Life Science Research Institute
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Abstract

This application discloses a kind of gene sequencing chip loading devices.The gene sequencing chip loading device of the application, including electric control module, several reagent bottles and at least gene sequencing chip charging assembly, gene sequencing chip charging assembly include reagent trough, topping-up pump and positive displacement pump all the way;Reagent trough, which is erect, to be set, and has the cavity that vertical size is adapted with gene sequencing chip, reagent trough top end opening;Topping-up pump is connected by rotary valve with each reagent bottle;Fluid injection pump output terminal is connected with reagent trough liquid injection port;Positive displacement pump is connected with reagent trough leakage fluid dram;Electric control module independent control topping-up pump, positive displacement pump and rotary valve.The gene sequencing chip loading device of the application is loaded using vertical immersion type, it is entirely avoided negative-pressure adsorption is unstable and fluid injection positive pressure excessively high the problem of bringing, and impregnates loading and works well;Also, due to being not required negative pressure adsorption module and additional positive pressure loading device, equipment cost lower.

Description

A kind of gene sequencing chip loading device
Technical field
This application involves gene sequencing chipset field, more particularly to a kind of gene sequencing chip loading device.
Background technology
Existing gene sequencing chip loading device, typically by the absorption of gene sequencing chip level on pedestal, so Sequencing reagent is pumped into the seal cavity of gene sequencing chip using syringe pump afterwards.Wherein, gene sequencing chip absorption exists It is to be adsorbed by negative pressure on pedestal, is often present with because negative pressure module goes wrong, and causes gene sequencing chip can not It is adsorbed or is adsorbed and is not accurate enough;However, the runner hole on gene sequencing chip must runner hole phase corresponding on pedestal It is corresponding, it is necessary to ensure that gene sequencing chip can be accurately located on chip base at this time, it otherwise will be due to positioning not It is accurate and lead to not load.
In addition, the seal cavity of gene sequencing chip, is to realize gene sequencing chip by very thin sheet glass and outline border Sealing, existing gene sequencing chip loading device, when carrying out sequencing reagent pumping using syringe pump, positive pressure easily makes glass Glass piece crushes, and influences normally to be sequenced.In order to solve this problem, the method generally used at present is, additional on the glass sheet to apply One positive pressure, for offsetting the positive pressure of syringe pump, protective glass piece will not be ruptured when injecting reagent;It is to be understood that volume again Add instrument cost outside.
The content of the invention
The purpose of the application is to provide a kind of improved gene sequencing chip loading device of structure.
The application employs following technical scheme:
This application discloses a kind of gene sequencing chip loading device, including electric control module 1, several reagent bottles 21st, 22 and 23 and at least gene sequencing chip charging assembly all the way, wherein per roadbed because sequence testing chip charging assembly all includes Reagent trough 3, topping-up pump 4 and positive displacement pump 5;Reagent trough 3, which is erect, to be set, and has the cavity stood vertically, the size and base of the cavity Because sequence testing chip is adapted, 3 top end opening of reagent trough facilitates gene sequencing Chip Vertical to be inserted into the cavity of reagent trough 3;Topping-up pump 4 are connected by rotary valve 6 with each reagent bottle, and the control topping-up pump 4 of rotary valve 6 is connected or disconnected with each reagent bottle;Topping-up pump 4 Output terminal is connected with the liquid injection port 31 of reagent trough 3, for injecting reagent for the cavity of reagent trough 3, in order to gene sequencing core Piece is impregnated;Positive displacement pump 5 is connected with the leakage fluid dram 32 of reagent trough 3, for the reagent in 3 cavity of reagent trough to be discharged;Electrically Control module 1 is electrically connected respectively with topping-up pump 4, positive displacement pump 5 and rotary valve 6 or signal is connected, independent control topping-up pump 4, row Liquid pump 5 is turned on and off, and the rotation of independent control rotary valve 6 makes selected reagent bottle be connected with topping-up pump 4.Wherein, reagent The number of bottle in a kind of realization method of the application, uses in total depending on reagent to be added needed for gene sequencing chip Three reagent bottles;In the application, erect or stand vertically for being all relative level.
It should be noted that the gene sequencing chip loading device of the application, key is using the side vertically impregnated Formula carries out gene sequencing chip loading, and reagent is held in reagent trough 3, and gene sequencing Chip Vertical is inserted, and impregnates liquid feeding;This Kind load mode, it is entirely avoided negative-pressure adsorption failure is brought without considering the problems of that syringe pump positive pressure is excessive, and is loaded It works well, it is only necessary to adjust the reagent needed for rotary valve selection, adjust the fluid injection speed of topping-up pump, and set what is vertically impregnated Time, whole flow process high degree of automation improve the loading efficiency of gene sequencing chip.Also, the gene of the application Sequence testing chip loading device, due to without the concern for negative pressure and positive pressure, without negative pressure adsorption module, also adding without additional positive pressure It carries and puts, equipment cost can be substantially reduced.
It should also be noted that, the key of the application is vertically to impregnate the loading of gene sequencing chip, as electrical control Module, main function are control topping-up pump 4, positive displacement pump 5 and rotary valve 6, may be referred to existing electric controling mode, herein It is not specifically limited.Gene sequencing chip is inserted and removed from, and can also refer to existing gene sequencing chip loading device, example It is such as inserted and removed from by manually or mechanically hand, is not specifically limited herein.
Preferably, the gene sequencing chip loading device of the application further includes waste collection bottle 7, waste collection bottle 7 and row The output terminal connection of liquid pump 5, for collecting the reagent discharged from reagent trough 3.
Preferably, two layers up and down of the gene sequencing chip loading device point of the application, is separated by panel 8,8 lower section of panel with It is lower floor between bottom plate 9, the top of panel 8 is upper strata;It is propped up between panel 8 and bottom plate 9 by support column 10, electric control module 1 is installed between panel 8 and bottom plate 9, i.e. lower floor;Lower floor's surrounding side plate 11 is crowded around, for protecting electric control module 1;Base Because sequence testing chip charging assembly is installed on 8 upper surface of panel, i.e. upper strata.
Preferably, the upper surface of panel 8 is provided with reagent trough mounting seat 12, reagent trough peace positioned at position in the middle Dress pedestal 12 is propped up reagent trough installation panel 14 by reagent trough pillar 13, and reagent trough 3 is fixedly installed in reagent trough mounting surface On plate 14;Topping-up pump 4 and positive displacement pump 5 are respectively arranged in the both sides of reagent trough 3.
It should be noted that the purpose of reagent trough pillar 13 is primarily to reagent trough 3 is supported, coordination and fluid injection The setting height(from bottom) of pump 4 and positive displacement pump 5, also, reagent trough 3 is independent is supported by reagent trough pillar 13, has also ensured that it is steady It is qualitative, reagent trough 3 is avoided to be influenced to generate vibrations by topping-up pump 4 and positive displacement pump 5, so as to influence gene sequencing chip.
Preferably, topping-up pump 4 is fixedly installed in the one of reagent trough 3 by topping-up pump mounting seat 41 and topping-up pump side plate 42 Side, the setting of topping-up pump side plate 42 are fixed in topping-up pump mounting seat 41, and topping-up pump 4 is fixedly mounted on topping-up pump side plate 42; Positive displacement pump 5 is fixedly installed in the opposite side of reagent trough 3, positive displacement pump side plate by positive displacement pump mounting seat 51 and positive displacement pump side plate 52 52 settings are fixed in positive displacement pump mounting seat 51, and positive displacement pump 5 is fixedly mounted on positive displacement pump side plate 52.
It should be noted that topping-up pump 4 is fixedly mounted on topping-up pump side plate 42, positive displacement pump 5 is fixedly mounted on positive displacement pump On side plate 52, it is on the one hand to the setting height(from bottom) for coordinating reagent trough 3, topping-up pump 4 and positive displacement pump 5, is in order to true on the other hand Protecting syringe pump and positive displacement pump, stabilization is not vibrated in the process of running, and the vibration of syringe pump and positive displacement pump in other words does not interfere with it Its component.
Preferably, gene sequencing chip loading device has six roadbeds because of sequence testing chip charging assembly, six 3 rows of reagent trough In a row, six topping-up pumps 4 are arranged in a row positioned at the one side of reagent trough 3, and six positive displacement pumps 5 are arranged in a row positioned at reagent trough 3 Opposite side.
It should be noted that in a kind of realization method of the application, gene sequencing chip loading device devises six tunnels altogether Gene sequencing chip charging assembly can be carried out at the same time the loading of six road immersion type gene sequencing chips, and the road needed also may be selected Number.Certainly, it can also be designed with more ways according to use demand, be not specifically limited herein.
Preferably, the gene sequencing chip loading device of the application further includes topping-up pump protective cover and positive displacement pump protective cover 16, topping-up pump protective cover covers in six topping-up pumps 4 wherein, for protecting topping-up pump 4;Positive displacement pump protective cover 16 is by six drains Pump 5 covers in wherein, for protecting positive displacement pump 5.
The advantageous effect of the application is:
The gene sequencing chip loading device of the application is loaded, it is entirely avoided negative-pressure adsorption is not using vertical immersion type Stablize and fluid injection positive pressure excessively high the problem of bringing, impregnate loading and work well;Also, since negative pressure adsorption module and volume is not required Outer positive pressure loading device, equipment cost are lower.
Description of the drawings
Fig. 1 is the structure diagram of gene sequencing chip loading device in the embodiment of the present application;
Fig. 2 is the structure diagram at another visual angle of gene sequencing chip loading device in the embodiment of the present application;
Fig. 3 is the structure diagram at another visual angle of gene sequencing chip loading device in the embodiment of the present application;
Fig. 4 is the structure diagram that gene sequencing chip loading device fluid controls in the embodiment of the present application.
Specific embodiment
Gene sequencing chip, is directly inserted into and is immersed in reagent trough by the gene sequencing chip loading device of the application, Reagent is directly pumped into reagent trough by topping-up pump, need not be by base compared with existing gene sequencing chip loading device Because sequence testing chip negative-pressure adsorption is on pedestal, also completely dispenses with and consider the runner hole on gene sequencing chip and the runner on pedestal The problem of hole is alignd, therefore, operates simpler convenience.Also, topping-up pump is that reagent is pumped into reagent trough, and there is no notes The problem of liquid pump positive pressure makes the sheet glass of gene sequencing chip broken.
The application is described in further detail below by specific embodiment.Following embodiment is only to the application into traveling One step illustrates, should not be construed as the limitation to the application.
Embodiment
The gene sequencing chip loading device of this example, is loaded onto available for by DNA sample on gene sequencing chip, can also For carrying out gene sequencing reaction, as shown in Figure 1 to Figure 3, the device of this example include 1, three reagent bottle 21 of electric control module, 22 and 23, switching valve 6,7 and six roadbed of waste collection bottle because of sequence testing chip charging assembly, wherein per roadbed because of sequence testing chip Charging assembly all includes reagent trough 3, topping-up pump 4 and positive displacement pump 5;Reagent trough 3, which is erect, to be set, and has the cavity stood vertically, should The size of cavity is adapted with gene sequencing chip 01, and 3 top end opening of reagent trough facilitates gene sequencing chip 01 to be inserted perpendicularly into examination The cavity of agent slot 3.
Topping-up pump 4, positive displacement pump 5 and reagent trough 3 form fluid passage, as shown in figure 4, topping-up pump 4 is by rotary valve 6 and respectively Reagent bottle connects, and the control topping-up pump 4 of rotary valve 6 is connected or disconnected with each reagent bottle;The output terminal of topping-up pump 4 and reagent trough 3 Liquid injection port 31 connect, for for the cavity of reagent trough 3 inject reagent, in order to be impregnated to gene sequencing chip;Drain Pump 5 is connected with the leakage fluid dram 32 of reagent trough 3, for the reagent in 3 cavity of reagent trough to be discharged.Wherein, liquid injection port 31 is opened in On the side wall on 3 top of reagent trough, liquid injection port 31 is connected with the cavity of reagent trough 3, for fluid injection;Leakage fluid dram 32 is opened in reagent trough On the side wall of 3 lower parts or the bottom of reagent trough 3 is opened in, leakage fluid dram 32 is connected with the cavity of reagent trough 3, for drain.It is useless Liquid receiving flask 7 is connected with the output terminal of positive displacement pump 5, for collecting the reagent discharged from reagent trough 3.Six roadbeds are because of sequence testing chip The waste liquid of the reagent trough of charging assembly can be collected into same waste collection bottle, can also be collected into different give up according to demand In liquid receiving flask or multiple waste collection bottles are used, different types of waste liquid is collected respectively, is not specifically limited herein.
The electric control module 1 of this example is electrically connected respectively with topping-up pump 4, positive displacement pump 5 and rotary valve 6 or signal is connected, solely Vertical control topping-up pump 4, positive displacement pump 5 are turned on and off, and the rotation of independent control rotary valve 6 makes selected reagent bottle and fluid injection 4 connection of pump.The effect of electric control module 1 is to coordinate control topping-up pump 4, positive displacement pump 5 and rotary valve 6, control principle and side Formula may be referred to existing electrical control, be not specifically limited herein.The control six of the electric control module 1 of this example independently Roadbed is because of six topping-up pumps or positive displacement pump of sequence testing chip charging assembly;It can select to open wherein some or certain several fluid injections Pump selects the way needed to carry out gene sequencing chip loading;It can also select to open wherein some or certain several positive displacement pumps, Reagent in corresponding reagent slot is discharged.
The gene sequencing chip loading device of this example, in improved frame mode, whole gene sequence testing chip loading device It two layers above and below point, is separated by panel 8, is lower floor between 8 lower section of panel and bottom plate 9, the top of panel 8 is upper strata;Panel 8 and bottom It is propped up between plate 9 by support column 10, electric control module 1 is installed between panel 8 and bottom plate 9, i.e. lower floor;Lower floor's surrounding side Plate 11 is crowded around, for protecting electric control module 1;Gene sequencing chip charging assembly is installed on 8 upper surface of panel, i.e. upper strata. The upper surface of panel 8 is provided with reagent trough mounting seat 12 positioned at position in the middle, and reagent trough mounting seat 12 passes through reagent Slot pillar 13 props up reagent trough installation panel 14, and six roadbeds are arranged in a row because of six reagent troughs 3 of sequence testing chip charging assembly It is fixedly installed in reagent trough installation panel 14;Topping-up pump 4 and positive displacement pump 5 are respectively arranged in the both sides of reagent trough 3, specifically, Topping-up pump 4 is fixedly installed in the one side of reagent trough 3, six fluid injection pump sides by topping-up pump mounting seat 41 and topping-up pump side plate 42 Plate 42 is erect be fixed in topping-up pump mounting seat 41 respectively, and six roadbeds divide because of six topping-up pumps 4 of sequence testing chip charging assembly It is not fixedly mounted on six topping-up pump side plates 42;Likewise, positive displacement pump 5 passes through positive displacement pump mounting seat 51 and positive displacement pump side plate 52 are fixedly installed in the opposite side of reagent trough 3, and six positive displacement pump side plates 52 are erect be fixed in positive displacement pump mounting seat 51 respectively, Six roadbeds are fixedly mounted on because of six positive displacement pumps 5 of sequence testing chip charging assembly on positive displacement pump side plate 52.Six topping-up pumps 4 are lined up In the one side of reagent trough 3, six positive displacement pumps 5 are arranged in a row the opposite side positioned at reagent trough 3 for one ranking.This example is further improved In mode, topping-up pump protective cover and positive displacement pump protective cover 16 are also provided with, six topping-up pumps 4 are covered in it by topping-up pump protective cover In, for protecting topping-up pump 4;Positive displacement pump protective cover 16 covers in six positive displacement pumps 5 wherein, for protecting positive displacement pump 5.
The gene sequencing chip loading device of this example carries out gene sequencing chip loading, by base using vertical immersion way Because sequence testing chip 01 is inserted into reagent trough, negative-pressure adsorption is not required, adds caused by avoiding negative-pressure adsorption shakiness or inaccuracy Load problem;Also, topping-up pump is directly to inject reagent in reagent trough, and non-implanted gene sequencing chip, therefore, avoids note Influence of the liquid pump positive pressure to chip or sheet glass.In addition, the gene sequencing chip loading device of this example, since negative pressure being not required to inhale Attached module, it is not required that additional positive pressure load-on module, therefore, equipment and maintenance cost are low.The gene sequencing chip of this example adds It carries and puts, different operations, such as injection DNA reagents can carry out gene sequencing chip according to the reagent difference injected, DNA sample is loaded onto on gene sequencing chip or injected sequencing reaction reagent, carries out gene sequencing, is not done herein specific It limits.
The foregoing is a further detailed description of the present application in conjunction with specific implementation manners, it is impossible to assert this Shen Specific implementation please is confined to these explanations.For those of ordinary skill in the art to which this application belongs, do not taking off On the premise of conceiving from the application, several simple deduction or replace can also be made.

Claims (7)

1. a kind of gene sequencing chip loading device, it is characterised in that:Including electric control module (1), several reagent bottles (21,22,23) and at least gene sequencing chip charging assembly, the gene sequencing chip charging assembly include reagent trough all the way (3), topping-up pump (4) and positive displacement pump (5);
The reagent trough (3), which is erect, to be set, and has vertical cavity, the size of the cavity is mutually fitted with the gene sequencing chip Should, reagent trough (3) top end opening facilitates the gene sequencing Chip Vertical to be inserted into the cavity of reagent trough (3);
The topping-up pump (4) is connected by rotary valve (6) with each reagent bottle, rotary valve (6) control topping-up pump (4) and each reagent bottle Connect or disconnect;The output terminal of topping-up pump (4) is connected with the liquid injection port (31) of the reagent trough (3), for for reagent trough (3) Reagent is injected, in order to be impregnated to the gene sequencing chip;
The positive displacement pump (5) connects with the leakage fluid dram (32) of the reagent trough (3), for the reagent in reagent trough (3) to be discharged;
The electric control module (1) is connected respectively with the electrical connection of topping-up pump (4), positive displacement pump (5) and rotary valve (6) or signal, Independent control topping-up pump (4), positive displacement pump (5) are turned on and off, and the rotation of independent control rotary valve (6) makes selected reagent Bottle is connected with topping-up pump (4).
2. gene sequencing chip loading device according to claim 1, it is characterised in that:Waste collection bottle (7) is further included, The waste collection bottle (7) connects with the output terminal of the positive displacement pump (5), for collecting the discharge from the reagent trough (3) Reagent.
3. gene sequencing chip loading device according to claim 1, it is characterised in that:The gene sequencing chip loading Two layers up and down of device point, is separated by panel (8), and being lower floor between bottom plate (9) below panel (8), the top of panel (8) is upper Layer;
It is propped up between panel (8) and bottom plate (9) by support column (10), the electric control module (1) is installed on panel (8) and bottom Between plate (9), i.e. lower floor;Lower floor's surrounding is crowded around with side plate (11), for protecting electric control module (1);
The gene sequencing chip charging assembly is installed on panel (8) upper surface, i.e. upper strata.
4. gene sequencing chip loading device according to claim 3, it is characterised in that:The upper surface of the panel (8), Position in the middle is provided with reagent trough mounting seat (12), reagent trough mounting seat (12) passes through reagent trough pillar (13) Reagent trough installation panel (14) is propped up, the reagent trough (3) is fixedly installed on the reagent trough installation panel (14);Fluid injection Pump (4) and positive displacement pump (5) are respectively arranged in the both sides of the reagent trough (3).
5. gene sequencing chip loading device according to claim 4, it is characterised in that:The topping-up pump (4) passes through note Liquid pump mounting seat (41) and topping-up pump side plate (42) are fixedly installed in the one side of the reagent trough (3), topping-up pump side plate (42) Setting is fixed in topping-up pump mounting seat (41), and topping-up pump (4) is fixedly mounted on topping-up pump side plate (42);The positive displacement pump (5) opposite side of the reagent trough (3), drain are fixedly installed in by positive displacement pump mounting seat (51) and positive displacement pump side plate (52) Pump side plate (52) setting is fixed in positive displacement pump mounting seat (51), and positive displacement pump (5) is fixedly mounted on positive displacement pump side plate (52).
6. gene sequencing chip loading device according to claim 5, it is characterised in that:The gene sequencing chip loading Device has six roadbeds because sequence testing chip charging assembly, six reagent troughs (3) are arranged in a row, and six topping-up pumps (4) are arranged in a row Positioned at the one side of reagent trough (3), six positive displacement pumps (5) are arranged in a row the opposite side positioned at reagent trough (3).
7. gene sequencing chip loading device according to claim 6, it is characterised in that:Further include topping-up pump protective cover and Positive displacement pump protective cover (16), the topping-up pump protective cover covers in six topping-up pumps (4) wherein, for protecting topping-up pump (4);Institute It states positive displacement pump protective cover (16) to cover in six positive displacement pumps (5) wherein, for protecting positive displacement pump (5).
CN201721280542.4U 2017-09-30 2017-09-30 A kind of gene sequencing chip loading device Active CN207452077U (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110734851A (en) * 2018-07-19 2020-01-31 深圳华大生命科学研究院 Liquid changing device and gene sequencer
CN110794566A (en) * 2018-08-01 2020-02-14 深圳华大生命科学研究院 Positioning device, optical imaging system and assembling method thereof
CN110835598A (en) * 2018-08-16 2020-02-25 深圳华大智造科技有限公司 Loading device, loading method and gene sequencing system
WO2020103126A1 (en) * 2018-11-23 2020-05-28 深圳华大智造科技有限公司 Gene sequencing reaction platform, sequencing chip and related method and system
CN112955536A (en) * 2018-08-16 2021-06-11 深圳华大智造科技有限公司 Handle device, positioning device, loading device and gene sequencer
WO2024027121A1 (en) * 2022-08-03 2024-02-08 深圳赛陆医疗科技有限公司 Biochemical reaction apparatus and nucleic acid sequencing device
WO2024098385A1 (en) * 2022-11-11 2024-05-16 深圳华大智造科技股份有限公司 Fluid transportation system, gene sequencer, and fluid transportation method

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110734851A (en) * 2018-07-19 2020-01-31 深圳华大生命科学研究院 Liquid changing device and gene sequencer
CN110734851B (en) * 2018-07-19 2023-03-14 深圳华大生命科学研究院 Liquid changing device and gene sequencer
CN110794566A (en) * 2018-08-01 2020-02-14 深圳华大生命科学研究院 Positioning device, optical imaging system and assembling method thereof
CN110794566B (en) * 2018-08-01 2021-09-07 深圳华大生命科学研究院 Positioning device, optical imaging system and assembling method thereof
CN110835598B (en) * 2018-08-16 2023-04-21 深圳华大智造科技股份有限公司 Loading device, loading method and gene sequencing system
CN110835598A (en) * 2018-08-16 2020-02-25 深圳华大智造科技有限公司 Loading device, loading method and gene sequencing system
CN112955536A (en) * 2018-08-16 2021-06-11 深圳华大智造科技有限公司 Handle device, positioning device, loading device and gene sequencer
CN112955536B (en) * 2018-08-16 2024-05-17 深圳华大智造科技股份有限公司 Handle device, positioning device, loading device and gene sequencer
WO2020103126A1 (en) * 2018-11-23 2020-05-28 深圳华大智造科技有限公司 Gene sequencing reaction platform, sequencing chip and related method and system
CN113242904B (en) * 2018-11-23 2024-04-30 深圳华大智造科技股份有限公司 Gene sequencing reaction platform, sequencing chip, and related methods and systems
CN113242904A (en) * 2018-11-23 2021-08-10 深圳华大智造科技股份有限公司 Gene sequencing reaction platform, sequencing chip, and related method and system
WO2024027121A1 (en) * 2022-08-03 2024-02-08 深圳赛陆医疗科技有限公司 Biochemical reaction apparatus and nucleic acid sequencing device
WO2024098385A1 (en) * 2022-11-11 2024-05-16 深圳华大智造科技股份有限公司 Fluid transportation system, gene sequencer, and fluid transportation method

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