Animal organism sample collection box and its suit
Technical field
The utility model belongs to biological technical field, especially belongs to animal cloning technology field such as animal somatic cell gram
Grand technical field, is related to a kind of biological sample collection box, for example can be used for cloning animal somatic cell more particularly to a kind of
For the collecting cassette of biological sample collection.The application further relate to include above-mentioned biological sample collection box for such as animal gram
The biological sample collection suit of grand such as cloning animal somatic cell.
Background technology
Animal cloning is a kind of technology that vegetative propagation is carried out by nuclear transfer process.Without sexual reproduction, and
It is the technology that genetic structure and cell nucleus donor same animals individual are produced by nuclear transfer, is just called animal cloning.
J.B.S.Haldane in 1963 is adopted in the speech of entitled " human race is in the biological possibility 20,000 years following "
With the term of " clone (Clone) ", the animal embryo cell of mesoderm growing early stage or the body cell of adults are moved through micrurgy
After planting in the egg mother cell for remove nucleus, under suitable condition, normal fetus can be developed into again.This embryo
It is transplanted among parent similar in the cyclostage, intact animal individual can be budded into.What is generated by nuclear transfer is dynamic
Object, genetic structure are identical with cell nucleus donor.
The birth of clone sheep " more profits " has started clone's research boom in the whole world, then, the report in relation to cloned animal
In rapid succession;1 month after being born in March, 1997, i.e. " more jasmines ", the U.S., TaiWan, China and Australian Scientists are delivered respectively
The message of their successful clone monkeys, pig and ox.But, they are cloned using embryonic cell, and meaning cannot
Compared with " more jasmines ".July in the same year, Roslyn research institute and the declaration of PPL companies are with the fetal fibroblast gram of genetic modification
It is grand go out in the world the first headband have the Transgenic Sheep " Polly " (Polly) of human gene.This achievement shows clone technology
Huge applications value in terms of transgenic animals are cultivated.
In July, 1998, Hawaii, America university Wakayama etc. reports, by mouse ovarian cumulus cell clone 27 survive it is small
Mouse, wherein 7 simply by the offspring of Cloning of mouse's time cloning again, this is after " more jasmines " later second batch mammalian somatic cell
Nuclear transfer offspring.In addition, Wakayama et al. is employed, different from " more jasmines ", new, relatively simple and success rate is higher
Clone technology, this technology is named as " Honolulu technology " with the university location.
Hereafter, the states such as the U.S., France, Holland and South Korea scientist also reports Somatic cell cloned calf and successfully disappears in succession
Breath;The research enthusiasm of Japanese Scientists is particularly surprising, in July, 1998 in April, 1999, Tokyo Agricultural Univ., nearly Ji universities, family
It is numerous and confused to raise improvement cause group, local domestic animal test site and private enterprise (such as maximum Snow Brand Milk Products of milk goods companies of Japan)
It reports, they use the achievement of the mammary glandular cell clened cows extracted in the ears of an ox or cow portion, buttocks muscles, cumulus cell and colostrum.
To the end of the year 1999, the whole world has 6 type cells --- and fetal fibroblast, mammary glandular cell, cumulus cell, fallopian tubal/
The somatic cell clone offspring of uterine epithelial cell, muscle cell and ear skin cell is successfully born.
In June, 2000, NW China agriculture and forestry science and technology university are gone out two " clone sheep " using adult goat somatic cell clone, but
Wherein one is early died young due to respiratory system depauperation.It is introduced that used clone technology is oneself research institute of the seminar
, entirely different with the technology of clone " more jasmines ", this shows that China scientist has also grasped the sophisticated technology of somatic cell clone.
Nuclear transfer experiments, which are carried out, in different inter-species also achieves some gratifying results, in January, 1998, University of Wisconsin-Madison
For the scientists of one Madison university of star using the ovum of ox as receptor, successful clone goes out five kinds of pig, ox, sheep, mouse and macaque lactations
The embryo of animal, this result of study show that the unfertilized egg of some species can be the same as the mature cell core for being derived from many animals
It is combined.Although these embryos miscarry, it has made good try to the possibility of interspecies cloning.1999, the U.S.
Science family expenses ox ovum clones the embryo of rare animal argali;China scientist has also cloned the early stage of giant panda with rabbit ovum
Embryo, these achievements illustrate that clone technology is likely to become protection and saves a new way for animals on the brink of extinction.
Cloning animal somatic cell is a technology with huge research and commercial value, may be used on therapeutic cloning,
Disease model, human organ transplantation, Animal Transgenic research, endangered animal varieties protection, excellent domestic animal kind amplification etc.
Aspect.Particularly, cloning animal somatic cell is mainly reflected in the application in terms of its following four:(1) cultivate it is excellent poultry kind and
Productive experiment animal;(2) transgenic animals are produced;(3) produce human embryo stem cell and be used for cell and tissue replacement therapy;(4)
Animal species in imminent danger are replicated, preserve and propagate animal species resource.
It carries out cultivating excellent poultry kind and productive experiment animal using animal somatic cell, can be widely applied for actual life
In, such as the breeding to domestic pets, and with huge market potential.
The collecting cassette having disclosed at present has following several:One kind is for gathering and storing Cord blood, umbilical cord and placenta
Collecting cassette, be separated into upper chamber and lower chambers including box body, box cover, umbilical cord box and placenta box, tray interior, be respectively used to
Umbilical cord box and placenta box are stored, this structure is more complicated, is also not easy to medical staff's operation, it is often more important that, tray interior
There is no refrigeration effect, the collecting cassette is made to be not suitable for storage bleeding of the umbilicus, umbilical cord and placenta for a long time, it is also not readily transportable.Another kind is
For gathering, storing the collecting cassette of Cord blood and/or umbilical cord, which includes lower box body, box cover and inner liner support body, liner
Supporter is placed in lower box body, is provided with multiple rectangular recess in inner liner support body, groove for place acquisition Cord blood and/or
The utensil of umbilical cord;The collecting cassette does not have a carry hand, not readily portable and transport, also without refrigeration effect inside collecting cassette, and can only
Store Cord blood and umbilical cord, 3, also have some collecting cassettes in the market using drawer-type structure, structure is more complicated, inner matter
Product are not very clear, and for the collecting cassette, unfamiliar medical staff uses unusual inconvenience.
Therefore, a kind of biological sample collection box is provided for animalsomaticcellcloningtechnology field, be especially to provide a kind of dynamic
The collecting cassette of tissue sample acquisition is used in object cell clone, to improve the efficiency of cloning animal somatic cell work and reliable
Property, it is still that those skilled in the art urgently expect.
Utility model content
The purpose of this utility model is to provide a kind of biological sample collection boxes, and it is thin to be especially to provide a kind of such as animal body
For the collecting cassette of tissue sample acquisition in born of the same parents clone, with the efficiency of expectation raising cloning animal somatic cell work and reliably
Property.It has been found that being designed by the application, the one or more of these desired effects can be realized.The application consequently found that and
It is accomplished.
For this purpose, this application provides a kind of (such as available for cloning animal somatic cell) biological sample collection box, the lifes
Object sample collection box includes:Box body is renovated and separates elastomer;It is characterized in that,
Described renovate is covered including main cover and pair, and an edge on the first edge of main cover and edge on the box body is in that can open
It is connected with the integral type of closure, second edge corresponding with first edge and an edge of the secondary lid are in that can open in main cover
It is connected with the integral type of closure, so that main cover secondary lid when covering the box body can further make box body and renovate
The two covers conjunction closely;
The separation elastomer is embedded in the box body, is provided in the separation elastomer and is respectively used to fix following object
The groove of product:Two blue ice bag slots, sample cleaning tube seat, temperature controller slot, sample storage tube seat and sterile sampler slot;
The sterile sampler slot is arranged at the edge of the separation elastomer;
One indigo plant ice bag slot, sample cleaning tube seat, temperature controller slot, sample storage tube seat, another blue ice bag slot five
A groove, which is arranged in order, to be arranged in the separation elastomer, and being provided between five grooves makes the perforation of five connections recessed
Slot, the perforation groove cause the blue ice bag placed in blue ice bag slot to absorb the heat in remaining groove.
By the design of the application, the design of groove is penetrated through especially by the application so that the application biological sample is adopted
It is each in collection box to need to control temperature or the regional connectivity for monitoring temperature, so as to so that the application biological sample
It is each in collecting cassette to need to control temperature or readily make equalized temperature for monitoring the region of temperature.
According to the biological sample collection box of the application, which is characterized in that can also place nothing in the sterile sampler slot
Bacteria sampler and disposable sterilized gloves.These articles need not control temperature, and particularly these articles are no longer after sampling
It inserts in the biological sample collection box of the application.
On the other hand, this application provides a kind of (such as available for cloning animal somatic cell) biological sample collection sets
Dress, including biological sample collection box and the following article being positioned in the biological sample collection box:Two blue ice bags, samples
Cleaning pipe, temperature controller, sample memotron, sterile sampler slot;The biological sample collection box includes:Box body is renovated and separated
Elastomer;It is characterized in that,
Described renovate is covered including main cover and pair, and an edge on the first edge of main cover and edge on the box body is in that can open
It is connected with the integral type of closure, second edge corresponding with first edge and an edge of the secondary lid are in that can open in main cover
It is connected with the integral type of closure, so that main cover secondary lid when covering the box body can further make box body and renovate
The two covers conjunction closely;
The separation elastomer is embedded in the box body, is provided in the separation elastomer and is respectively used to fix two indigo plants
Ice bag, sample cleaning pipe, temperature controller, sample memotron, the groove of sterile sampler:Two blue ice bag slots, sample cleaning tube seat,
Temperature controller slot, sample storage tube seat and sterile sampler slot;
The sterile sampler slot is arranged at the edge of the separation elastomer;
One indigo plant ice bag slot, sample cleaning tube seat, temperature controller slot, sample storage tube seat, another blue ice bag slot five
A groove, which is arranged in order, to be arranged in the separation elastomer, and being provided between five grooves makes the perforation of five connections recessed
Slot, the perforation groove cause the blue ice bag placed in blue ice bag slot to absorb the heat in remaining groove.
It is set with according to the biological sample collection of the application, which is characterized in that be also placed with one in the sterile sampler slot
Secondary property sterile gloves.
In some embodiments of the application, separate elastomer and be separated out several grooves, be effectively protected in groove
Placed article makes it be hardly damaged in transportational process.The utensil placed in groove, after the completion of biological sample collection, sample
This memotron is played back in corresponding groove, and absorbing heat by blue ice bag makes biological sample may be at low temperature environment
In.
In some embodiments of the application, temperature controller for recording in real time, the temperature in preservation biological sample collection box
Degrees of data.
In some embodiments of the application, the various utensils placed in groove are respectively equipped with discrimination label, distinguish mark
Label are affixed directly to the outer surface of above-mentioned utensil, and medical staff or biological sample collection personnel is made to be not easy disorder in operation.
In some embodiments of the application, for blue ice bag as cold-storage device, cold accumulation effects are good, and biological sample is made to exist
Certain temperature is kept in transportational process, is hardly damaged.
In some embodiments of the application, on box body and/or box cover can configuration information posting field, for recording quilt
The identity information of acquisition target.
The advantageous effect of biological sample collection box provided by the utility model and acquisition suit is:The utility model bag
Cold-storage device is included, biological sample is made to keep certain temperature in transportational process, is hardly damaged;Penetrate through groove and device for monitoring temperature
Setting, can more effectively ensure the activity of biological sample;Discrimination label can be sticked on the article placed in groove, makes doctor
Shield personnel are not easy disorder in operation;On babinet and box body can configuration information posting field, for recording the body of gathered person
Part information, will not entanglement even if having multiple biological sample collections.
Description of the drawings
Fig. 1:The stereogram of the closure state of the application biological sample collection box.
Fig. 2:The side view of the closure state of the application biological sample collection box.
Fig. 3:The schematic diagram of object distributed areas in the application biological sample collection box.
Specific embodiment
The utility model can be further described by the following examples, however, the model of the utility model
It encloses and is not limited to following embodiments.One of skill in the art is it is understood that in the spirit and scope without departing substantially from the utility model
On the premise of, various change and modification can be carried out to the utility model.The utility model is to the material that is arrived used in experiment
And test method carries out general and/or specific description.Although to realize many materials used in the utility model aim
Material and operating method are it is known in the art that still the utility model is still described in detail as far as possible herein.
The various embodiments of the utility model are described in detail with reference to the accompanying drawings.
Fig. 1 depicts the stereogram of the closure state of the application biological sample collection box, and Fig. 2 depicts the application biology sample
The side view of the closure state of product collecting cassette, Fig. 3 depict the signal of object distributed areas in the application biological sample collection box
Figure.
Complex chart 1, Fig. 2 and Fig. 3 are discribed, on the one hand, in life of the application for example available for cloning animal somatic cell
In one exemplary implementation scheme of object sample collection box, which includes:Box body 11 is renovated 12 and is separated
Elastomer 13;Wherein:
It is described to renovate 12 and include main cover 121 and secondary lid 122, the first edge 123 of main cover 121 and edge on the box body 11
One edge connect with the integral type being closed in can open, in main cover 121 with 123 corresponding second edge 124 of first edge and
One edge of the pair lid 122 is in that can open to connect with the integral type being closed, so that main cover 121 is covering the box body
The pair lid 122 can further be such that box body 11 is closed with both 12 lids closely are renovated when 11;
The separation elastomer 13 is embedded in the box body 11, is provided in the separation elastomer 13 and is respectively used to fix
The groove of following article:Two blue ice bag slots 131, sample cleaning tube seat 132, temperature controller slot 133,134 and of sample storage tube seat
Sterile sampler slot 135;
The sterile sampler slot 135 is arranged at the edge of the separation elastomer 13;
It is one indigo plant ice bag slot 131, sample cleaning tube seat 132, temperature controller slot 133, sample storage tube seat 134, another
A indigo plant ice bag 131 5 grooves of slot, which are arranged in order, to be arranged in the separation elastomer 13, and is provided between five grooves
Make the perforation groove 136 of five connections, the perforation groove 136 is so that the blue ice bag placed in blue ice bag slot 131 is absorbed from it
Heat in remaining groove.
Complex chart 1, Fig. 2 and Fig. 3 are discribed, on the other hand, in one embodiment of the application, provide a kind of example
Biological sample collection such as available for cloning animal somatic cell is set with, and including biological sample collection box 1 and is positioned over the life
Following article in object sample collection box 1:Two blue ice bags, sample cleaning pipe, temperature controller, sample memotron, sterile samplers
(these articles are the articles of this field routine and can be obtained by commercially available or self-control that they do not carry out figure in this application to slot
Show and draw);The biological sample collection box 1 includes:Box body 11 renovates 12 and separates elastomer 13;Wherein:
It is described to renovate 12 and include main cover 121 and secondary lid 122, the first edge 123 of main cover 121 and edge on the box body 11
One edge connect with the integral type being closed in can open, in main cover 121 with 123 corresponding second edge 124 of first edge and
One edge of the pair lid 122 is in that can open to connect with the integral type being closed, so that main cover 121 is covering the box body
The pair lid 122 can further be such that box body 11 is closed with both 12 lids closely are renovated when 11;
The separation elastomer 13 is embedded in the box body 11, is provided in the separation elastomer 13 and is respectively used to fix
Two blue ice bags, sample cleaning pipe, temperature controller, sample memotron, the grooves of sterile sampler:Two blue ice bag slots 131, samples
Clean tube seat 132, temperature controller slot 133, sample storage tube seat 134 and sterile sampler slot 135;
The sterile sampler slot 135 is arranged at the edge of the separation elastomer 13;
It is one indigo plant ice bag slot 131, sample cleaning tube seat 132, temperature controller slot 133, sample storage tube seat 134, another
A indigo plant ice bag 131 5 grooves of slot, which are arranged in order, to be arranged in the separation elastomer 13, and is provided between five grooves
Make the perforation groove 136 of five connections, the perforation groove 136 is so that the blue ice bag placed in blue ice bag slot 131 is absorbed from it
Heat in remaining groove.
In some embodiments of the application, disposable sterilized gloves are also placed in the sterile sampler slot 135.
In some embodiments of the application, separate elastomer and be separated out several grooves, be effectively protected in groove
Placed article makes it be hardly damaged in transportational process.The utensil placed in groove, after the completion of biological sample collection, sample
This memotron is played back in corresponding groove, and absorbing heat by blue ice bag makes biological sample may be at low temperature environment
In.
In some embodiments of the application, temperature controller for recording in real time, the temperature in preservation biological sample collection box
Degrees of data.
In some embodiments of the application, the various utensils placed in groove are respectively equipped with discrimination label, distinguish mark
Label are affixed directly to the outer surface of above-mentioned utensil, and medical staff or biological sample collection personnel is made to be not easy disorder in operation.
In some embodiments of the application, for blue ice bag as cold-storage device, cold accumulation effects are good, and biological sample is made to exist
Certain temperature is kept in transportational process, is hardly damaged.
In some embodiments of the application, on box body and/or box cover can configuration information posting field, for recording quilt
The identity information of acquisition target.
Biological sample collection suit and biological sample collection box of the application for example available for cloning animal somatic cell are usual
Following method can be shone to use:Biological sample collection suit and biological sample collection box have been configured according to application scheme;Right
The blue ice bag is put in refrigerator-freezer in advance before animal progress biological sample collection and is freezed, the sample in being set with using biological sample collection
This cleaning pipe, sample memotron, sterile sampler carry out animal in the acquisition of biological sample, the sample through preliminary treatment after acquisition
Product are placed in sample memotron, are placed in biological sample collection box, while the blue ice bag freezed in advance is put back to biological sample and is adopted
Collect box in, sealing, when 24 is small it is interior be delivered to biological sample deeply handle and for example cloning animal somatic cell laboratory carry out
Processing.The biological sample collection suit and biological sample collection box of the application can make the biological sample of acquisition low what can be monitored
Time when 24 is small is placed at temperature.
By the design of the application, the design of groove is penetrated through especially by the application so that the application biological sample is adopted
It is each in collection box to need to control temperature or the regional connectivity for monitoring temperature, so as to so that the application biological sample
It is each in collecting cassette to need to control temperature or readily make equalized temperature for monitoring the region of temperature.
According to the biological sample collection box of the application, which is characterized in that can also be placed in the sterile sampler slot 135
Sterile sampler and disposable sterilized gloves.These articles need not control temperature, and particularly these articles are not after sampling
It inserts again in the biological sample collection box of the application.
In the description of the utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width
Degree ", " thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outer ", " suitable
The orientation or position relationship of the instructions such as hour hands ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " are based on orientation shown in the drawings
Or position relationship, be for only for ease of description the utility model and simplify description rather than instruction or imply signified device or
Element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that limit to the utility model
System.
In addition, term " first ", " second " are only used for description purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more this feature.In the description of the utility model, " multiple " are meant that two or two
More than, unless otherwise specifically defined.
In the utility model, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " Gu
It is fixed " etc. terms should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or integral;Can be
Mechanical connection or electrical connection;It can be directly connected, can also be indirectly connected by intermediary, can be two
The interaction relationship of connection or two elements inside element.It for the ordinary skill in the art, can basis
Concrete condition understands concrete meaning of the above-mentioned term in the utility model.
In the utility model, unless otherwise clearly defined and limited, fisrt feature is "above" or "below" second feature
Can be that the first and second features contact directly or the first and second features pass through intermediary mediate contact.Moreover, first is special
Sign second feature " on ",
" top " and " above " but fisrt feature are directly over second feature or oblique upper or are merely representative of fisrt feature
Level height is higher than second feature.Fisrt feature second feature " under ", " lower section " and " below " can be that fisrt feature exists
Immediately below second feature or obliquely downward is merely representative of fisrt feature level height less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the utility model.In the present specification, to the schematic table of above-mentioned term
Identical embodiment or example need not be directed to by stating.Moreover, particular features, structures, materials, or characteristics described can be
It is combined in an appropriate manner in any one or more embodiments or example.In addition, without conflicting with each other, this field
Technical staff can carry out the different embodiments described in this specification or example and different embodiments or exemplary feature
With reference to and combination.
Embodiment described above is only the preferred embodiment to absolutely prove the utility model and being lifted, the utility model
Protection domain it is without being limited thereto.The equivalent substitute or change that those skilled in the art are made on the basis of the utility model
It changes, within the scope of protection of the utility model.The scope of protection of the utility model is subject to claims.