CN207380052U - A kind of vomitoxin quantitatively detects external member and kit - Google Patents
A kind of vomitoxin quantitatively detects external member and kit Download PDFInfo
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- CN207380052U CN207380052U CN201721094727.6U CN201721094727U CN207380052U CN 207380052 U CN207380052 U CN 207380052U CN 201721094727 U CN201721094727 U CN 201721094727U CN 207380052 U CN207380052 U CN 207380052U
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- tube body
- vomitoxin
- external member
- microballoon
- detection
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Abstract
The utility model discloses a kind of vomitoxins quantitatively to detect external member and kit, is related to kit field.Vomitoxin disclosed in the utility model quantitatively detect external member by time-resolved fluorescence microballoon be placed in by diaphragm seal, tube body and in accommodating chamber that inner wall below the annular flange and the bottom wall of tube body are formed, it can prevent from helping avoid the waste of material since powdered time-resolved fluorescence microballoon caused by pitch is distributed to body open or is attached on inboard wall of tube body during transport.
Description
Technical field
The utility model is related to kit fields, and external member and reagent are quantitatively detected in particular to a kind of vomitoxin
Box.
Background technology
Vomitoxin (vomitoxin), also known as deoxynivalenol (DON), chemistry entitled 3 α, 7 α, 15 13
Hydroxyl grass Fusariumsp -9- alkene -8- ketone belongs to trichothecene.It gains the name since it can cause the vomiting of pig, to people
Body has certain damaging effect, and European Union's criteria for classification is three-level carcinogenic substance.
The pollution of vomitoxin has caused the great attention of people, but the feed and feedstuff vomitoxin of many countries
Limit standard and pollution level criterion are still imperfect, also disunity.As the U.S. has formulated feed cereal and its byproduct
(in addition to corn) vomitoxin Permissible limit≤5mg/kg, European Union formulate corn and its byproduct Permissible limit≤1.75mg/kg.
China has formulated the limit standard of vomitoxin in part mixed feed (e.g., pig, fowl and calf), but does not formulate feedstuff
Vomitoxin limit standard in (in addition to DDGS).
The detection method of vomitoxin has thin-layered chromatography (TLC), high performance liquid chromatography (HPLC), column purification method to combine
Gas chromatography (GC/ECD), high performance liquid chromatography tandem mass spectrum method (HPLC-MS/MS), the radioactivity of electron capture detector
Immunoassay (RIA) etc..
Immunochromatography (lateral flow immunoassay, LFIA) Rapid detection test strip is mostly with colloidal gold, colour
Emulsion particle or fluorescein are as label.Quick detection product based on colloidal gold-labeled method exploitation, there are sensitivity
It is low, can only qualitative or sxemiquantitative, the problems such as differences between batches are larger;Although coloured latex particle difference between batch improves to some extent, spirit
Sensitivity is still relatively low, also can only qualitative or sxemiquantitative;Immunochromatography sensitivity based on fluorescein labelling technique has larger carry
Height can also carry out quantitative detection, but due to containing higher autofluorescent background signal in sample, and stock displacements are smaller, it can be right
Detection generates large effect.
Time-resolved fluorescence (Time-resolved Fluorescence, TRF) is a kind of heterotope fluorescent marker,
Compared with common fluorescent, there is the features such as stock displacements are big, and fluorescence lifetime is long, can effectively avoid the background in sample glimmering
The influence of the stray lights such as light and exciting light.It uses lanthanide series labelled antigen or antibody, according to the hair of lanthanide chelate
Light feature measures fluorescence with TIME RESOLVED TECHNIQUE, while two parameters of Detection wavelength and time carry out signal resolution, can be effectively
The interference of non-specific fluorescence is excluded, there is higher sensitivity and antijamming capability compared to common fluorescent.Since it is highly sensitive,
It is clinically widely used, gradually instead of radiommunoassay.
Presently, there are the detection methods combined using time-resolved fluorescence detection technique and immunochromatography technique, are mainly
Antigen or antibody are separately coated on the different position of test strips with time-resolved fluorescence microballoon, when detection adds sample to be tested
Onto test strips, the target substance allowed in sample to be tested is reacted with antigen or antibody in test strips, during chromatography, when
Between resolved fluorometric microballoon be just attached on antigen or antibody, such detection mode be unfavorable for for many kinds of substance while examine
It surveys, and determinand has the problem of reaction is insufficient with antigen or antibody in reaction in test strips.
Utility model content
The purpose of this utility model is to provide a kind of vomitoxins quantitatively to detect external member, which quantitatively detects set
It is housed in part containing the detection microballoon that different vomitoxin antibody are tagged to time-resolved fluorescence microballoon in advance, detection microballoon
It in tube body, is not coated in test strips, so as to when detecting, be easy to react with sample to be tested, ensures that reaction is more abundant.
The purpose of this utility model is to provide a kind of vomitoxin immue quantitative detection reagent box, including above-mentioned vomiting poison
Element quantitatively detects external member.
The utility model is realized in this way:
A kind of vomitoxin quantitatively detects external member, including eight unions and eight detection cards;
Eight unions have eight tube bodies being connected with each other and the pipe cap with the opening cooperation of tube body, and the inside of each tube body is deposited
It is placed with lyophilized in powdered time-resolved fluorescence microballoon, time-resolved fluorescence microballoon includes detection microballoon and Quality Control microballoon,
The fluorescent microsphere that microballoon is vomitoxin antibody mark is detected, Quality Control microballoon is the fluorescent microsphere of biotin labeling;
Detection card includes bottom plate, the nitrocellulose filter being arranged on bottom plate and is overlapped on nitrocellulose filter two respectively
The water absorption pad and sample pad at end are provided with the nature controlling line of coating Avidin and coating vomitoxin antibody on nitrocellulose filter
Detection line;
The circumferencial direction of the inner wall of tube body along tube body extends and the annular flange prominent to the center of circle direction of tube body, convex annular
Diaphragm seal is provided on edge, the periphery of diaphragm seal is connected along the circumferencial direction of tube body with annular flange, diaphragm seal, tube body and position
The bottom wall of inner wall and tube body below annular flange forms to accommodate the accommodating chamber of time-resolved fluorescence microballoon, time point
Distinguish that fluorescent microsphere is placed in accommodating chamber.
Further, in the utility model preferred embodiment, diaphragm seal is set on the surface of the opening of tube body
There is easy tear strip, one end of easy tear strip is fixedly connected with diaphragm seal, and the other end of easy tear strip prolongs to the opening direction of tube body
It stretches.
Further, in the utility model preferred embodiment, the upper surface of diaphragm seal and annular flange passes through viscous stick
Agent connects, and the position that easy tear strip corresponds to the inner wall of the close tube body of annular flange with diaphragm seal is fixedly connected.
Further, in the utility model preferred embodiment, the length L of easy tear strip is more than annular flange and opening
Edge height H.
Further, the sealing-plug for stretching into tubular body is provided in the utility model preferred embodiment, on pipe cap,
Sealing-plug is hemispherical.
Further, in the utility model preferred embodiment, bottom plate is plastic bottom board.
Further, in the utility model preferred embodiment, external member further includes the bottle with bottle cap, eight detections
Card and eight unions are placed in bottle.
A kind of vomitoxin immue quantitative detection reagent box quantitatively detects external member including multiple above-mentioned vomitoxins.
Further, in the utility model preferred embodiment, vomitoxin immue quantitative detection reagent box further includes packaging
Box body, multiple vomitoxins quantitatively detect external member and are contained in Packaging box body.
The beneficial effects of the utility model have:
Vomitoxin provided by the utility model quantitatively detects external member, blocks including eight unions and detection, and will be lyophilized
The fluorescent microsphere and Quality Control microballoon of detection microballoon, that is, vomitoxin antibody mark are placed in the tube body of eight unions, during detection, first will
Sample to be tested is reacted with time-resolved fluorescence microballoon in tube body, and then sample pad that reaction solution is added to detection card carries out layer
Analysis is read the signal of detection line and nature controlling line by instrument, further according to standard curve concentration, you can obtains vomitting poison in sample to be tested
The concentration of element, is reacted in tube body with time-resolved fluorescence microballoon due to sample to be tested rather than is carried out in test strips, so
The fluorescent microsphere that the vomitoxin in sample to be tested is marked with vomitoxin antibody is allowd to come into full contact with reaction, utmostly
The fluorescent microsphere that ground allows vomitoxin to be marked with vomitoxin antibody is combined, and then coated in the detection line being detected on card
Vomitoxin antibody capture is detected, and helps to improve the accuracy of testing result;
In addition, by time-resolved fluorescence microballoon be placed in by diaphragm seal, tube body and below the annular flange in
In the accommodating chamber that the bottom wall of wall and tube body is formed, it can prevent during transport due to powdered caused by pitch
Time-resolved fluorescence microballoon is distributed to body open or is attached on inboard wall of tube body, helps avoid the waste of material, and raising makes
The usage experience effect of user.
Description of the drawings
It, below will be to required use in embodiment in order to illustrate more clearly of the technical solution of the utility model embodiment
Attached drawing be briefly described, it should be understood that the following drawings illustrates only some embodiments of the utility model, therefore should not be by
Regard the restriction to scope as, for those of ordinary skill in the art, without creative efforts, may be used also
To obtain other relevant attached drawings according to these attached drawings.
Fig. 1 is the structure diagram for eight unions that embodiment 1 provides;
Fig. 2 is the cross-sectional view for the single tube body that embodiment 1 provides;
Fig. 3 is the top view of single tube body in Fig. 2;
Fig. 4 is the structure diagram at the first visual angle of the detection card that embodiment 1 provides;
Fig. 5 is the cross-sectional view that card is detected in Fig. 4.
Icon:Eight unions of 100-;110- tube bodies;111- is open;112- annular flanges;113- diaphragm seals;114- accommodating chambers;
The easy tear strips of 115-;120- pipe caps;121- sealing-plugs;200- detection cards;210- bottom plates;220- nitrocellulose filters;221- matter
Control line;222- detection lines;230- water absorption pads;240- sample pads.
Specific embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Attached drawing in type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model part of the embodiment, instead of all the embodiments.Usually here described in attached drawing and
The component of the utility model embodiment shown can configure to arrange and design with a variety of.
Therefore, requirement is not intended to limit to the detailed description of the embodiment of the utility model provided in the accompanying drawings below
The scope of the utility model of protection, but it is merely representative of the selected embodiment of the utility model.Based in the utility model
Embodiment, those of ordinary skill in the art's all other embodiments obtained without creative efforts, all
Belong to the scope of the utility model protection.
It should be noted that:Similar label and letter represents similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined, then it further need not be defined and explained in subsequent attached drawing in a attached drawing.
, it is necessary to explanation in the description of the utility model, term " " center ", " on ", " under ", it is "left", "right", " perpendicular
Directly ", the orientation of the instructions such as " level ", " interior ", " outer ", " clockwise ", " counterclockwise " or position relationship are based on shown in the drawings
Orientation or position relationship or the utility model product using when the orientation usually put or position relationship, merely to just
In description the utility model and simplified description rather than instruction or imply that signified device or element must be with specific sides
Position, with specific azimuth configuration and operation, therefore it is not intended that limitation to the utility model.
In addition, the terms such as term " level ", " vertical ", " pendency " are not offered as requiring component abswolute level or pendency, and
It is that can be slightly tilted.It is not to represent the structure if " level " only refers to that its direction is more horizontal with respect to for " vertical "
It has to fully horizontally, but can be slightly tilted.
In the description of the utility model, it is also necessary to which explanation, unless otherwise clearly defined and limited, term " are set
Put ", " installation ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected,
Or it is integrally connected;Can be mechanical connection or electrical connection;It can be directly connected, intermediary can also be passed through
It is indirectly connected, can be the connection inside two elements.For the ordinary skill in the art, can be managed with concrete condition
Solve concrete meaning of the above-mentioned term in the utility model.
Embodiment 1
With reference to figure 1- Fig. 4, a kind of vomitoxin provided in this embodiment quantitatively detects external member, including:One eight union
100th, eight detection cards 200 and the bottles for being used to receive the union and eight detection cards 200.
In the present embodiment, as depicted in figs. 1 and 2, eight unions 100 have be connected with each other eight tube bodies 110 and with pipe
The pipe cap 120 that the opening 111 of body 110 coordinates.The internal storage of each tube body 110 has lyophilized glimmering in powdered time resolution
Light microballoon.Time-resolved fluorescence microballoon includes detection microballoon and Quality Control microballoon.Microballoon is detected as the glimmering of vomitoxin antibody mark
Light microballoon, Quality Control microballoon are the fluorescent microsphere of biotin labeling.
In the present embodiment, the preparation method for detecting microballoon be can refer into following steps:
(1) time-resolved fluorescence microballoon is subjected to ultrasonic mixing with ultrasonic wave.
(2) 1ml time-resolved fluorescences microballoon is taken to add in 9ml purified waters, adds in 10mg NHS, adds in 5mg EDC, room temperature is anti-
Answer 1 hour.
(3) reactant for obtaining step 1.2 centrifuges, and supernatant is abandoned in 10000rpm, centrifugation 30 minutes, adds in 1ml purifying
Water, mixing.
(4) add in vomitoxin antibody 0.1mg, when normal-temperature reaction 2 is small, add in 1%BSA0.1ml, carry out closing 2 it is small when.
(5) reactant for obtaining step 1.4 centrifuges, and supernatant is abandoned in 10000rpm, centrifugation 30 minutes, is added in 1ml and is preserved
Liquid, mixing obtain the time-resolved fluorescence microspheres solution of vomitoxin antibody mark.Wherein, liquid is preserved by adding in 0.01M PBS
Enter 0.5%BSA and 0.1%Proclin300 (i.e. liquid bio preservative) is formulated.
(6) the time-resolved fluorescence microspheres solution that vomitoxin antibody marks is taken to be put into after freeze dryer freezes to get to use
In the detection microballoon of detection vomitoxin, itself and lyophilized Quality Control microballoon are mixed together, are packed into the tube body of eight unions 100
In 110.
In addition, in the present embodiment, as shown in Figure 4 and Figure 5, detection card 200 includes bottom plate 210, is arranged on bottom plate 210
Nitrocellulose filter 220 and the water absorption pad 230 at 220 both ends of nitrocellulose filter and sample pad 240 are overlapped on respectively, nitric acid is fine
The nature controlling line 221 i.e. C lines for including Avidin and the detection line 222 for being coated with vomitoxin antibody are provided on the plain film 220 of dimension
That is T lines.In the present embodiment, bottom plate 210 is plastic bottom board 210.
During detection, by detected sample (if liquid-type) directly or by the extracting solution obtained after the extraction of certain method
(in the case that sample to be tested is solid type, can according to said method handle sample to be tested), which is added in tube body 110, (to be detected sample volume to add
Entering amount can determine as the case may be, and the 3/5-4/5 using the total volume after addition as the volume of tube body 110 is advisable) it is reacted
(such as 5 minutes) for a period of time, then negate should after liquid be added to detection card 200 sample pad 240 chromatographed, a period of time
Afterwards, the fluorescence signal of the detection line 222 and nature controlling line 221 on detection card 200 is read by instrument, further according to standard curve concentration,
It can obtain the concentration of vomitoxin in sample to be tested.The reaction process is the prior art, and the utility model no longer does this in detail
Thin description.
Carry out antigen-antibody reaction in test strips compared to of the prior art, the utility model due to sample to be tested with
Time-resolved fluorescence microballoon, which is reacted in tube body 110 and is dispersed in liquid, to carry out, so that the vomiting in sample to be tested
The fluorescent microsphere that Venom antigens substance can be marked with vomitoxin antibody come into full contact with reaction, farthest allow vomitoxin
It is combined with the fluorescent microsphere of vomitoxin antibody mark, and then the vomitoxin antibody in the detection line 222 being detected on card 200
Capture is detected, and helps to improve the accuracy of testing result.
In addition, in order to avoid during transport due to powdered time-resolved fluorescence microballoon caused by pitch point
It is scattered to the opening of tube body 110 111 or is attached on 110 inner wall of tube body, the waste of material caused by, in the present embodiment, tube body
110 inner wall along tube body 110 circumferencial direction extension and the annular flange 112 prominent to the center of circle direction of tube body 110 (such as Fig. 1,
Shown in Fig. 2 and Fig. 3).
It is provided with diaphragm seal 113 in annular flange 112, the circumferencial direction and annular of the periphery of diaphragm seal 113 along tube body 110
Flange 112 connects.Diaphragm seal 113, tube body 110 and positioned at the inner wall of the lower section of annular flange 112 and the bottom wall structure of tube body 110
Into for accommodating the accommodating chamber 114 of time-resolved fluorescence microballoon.It is above-mentioned it is lyophilized be in powdered time-resolved fluorescence microballoon
It is placed in the accommodating chamber 114.Diaphragm seal 113 is made of polyethylene, and there is softness easily to tear characteristic.
Diaphragm seal 113 can be prevented effectively during transport due to powdered time resolution caused by pitch
Fluorescent microsphere is distributed to the opening of tube body 110 111 or is attached on 110 inner wall of tube body, helps avoid the waste of material, raising makes
The usage experience effect of user.
In addition, by diaphragm seal 113, tube body 110 and positioned at the inner wall of the lower section of annular flange 112 and the bottom of tube body 110
The limitation of time-resolved fluorescence microballoon can be concentrated on position of the tube body 110 by bottom wall by the accommodating chamber 114 that wall is formed well, be prevented
Only time-resolved fluorescence microsphere powder is attached on the inner wall of 110 higher position of tube body, is conducive to its later stage and sample to be tested
Mixing and contact improve the utilization rate of time-resolved fluorescence microsphere powder.
Diaphragm seal 113 is removed when in use for convenience, and in the present embodiment, diaphragm seal 113 is close to the opening of tube body 110
Easy tear strip 115 is provided on 111 surface, one end of easy tear strip 115 is fixedly connected with diaphragm seal 113, easy tear strip 115
111 direction of opening from the other end to tube body 110 extend.
In use, being removed by pullling easy tear strip 115, and then by diaphragm seal 113, expose time-resolved fluorescence microballoon,
Then sample to be tested is added in, is reacted.It can be convenient for users to removal diaphragm seal by the setting of easy tear strip 115
113。
In the present embodiment, diaphragm seal 113 is connected with the upper surface of annular flange 112 by viscous glutinous agent, easy tear strip 115
The position for corresponding to the inner wall of the close tube body 110 of annular flange 112 with diaphragm seal 113 is fixedly connected.
The tie point of easy tear strip 115 and diaphragm seal 113 is designed into the inner wall in the close tube body 110 of annular flange 112
Position, can enable when tearing diaphragm seal 113 by diaphragm seal 113 entirely remove, avoid remaining diaphragm seal 113 broken
The reaction of piece influence time resolved fluorometric microballoon and sample to be tested.
In the present embodiment, the length L of easy tear strip 115 is more than annular flange 112 and the height H at 111 edge of opening.It should
Design structure causes one end i.e. one end (or free end) away from diaphragm seal 113 of easy tear strip 115, can reach tube body
Outside 110, the free end is directly clamped convenient for user, pulls easy tear strip 115, and then quickly and easily removes diaphragm seal 113,
It efficiently avoids user and extend into the inconvenient problem that the easy tear strip 115 of gripping is brought in tube body 110 with hand or with clip.
It can be determined as the length L of specific easily tear strip 115 according to actual use demand, as long as more than annular flange 112 with opening
The height H at 111 edges of mouth.
Certainly, diaphragm seal 113 and easy tear strip 115 can be used flexible material and be made and can be integrally formed, for example with
Polyethylene film is made, and conveniently tears.In addition, in the present embodiment, eight union 100 is whole to be made of transparent plastic, convenient for seeing
Examine the reaction in tube body 110.
In the present embodiment, the sealing-plug 121 stretched into inside tube body 110 is provided on pipe cap 120, sealing-plug 121 is in half
It is spherical.When sealing-plug 121 is extend into tube body 110, the free end of easy tear strip 115 is presented with flexuosity.Certainly, each
121 quantity of sealing-plug set on pipe cap 120 is corresponded with 110 quantity of tube body.In the situation of 113 occasional breakage of diaphragm seal
Under, by the setting of sealing-plug 121, the time-resolved fluorescence microballoon in tube body 110 can further be prevented to be distributed to tube body 110
Opening 111 at or be attached to caused wasting phenomenon on 110 inner wall of tube body, improve the utilization rate of time-resolved fluorescence microballoon.
Embodiment 2
The present embodiment provides a kind of vomitoxin immue quantitative detection reagent box, including Packaging box body and multiple embodiments 1
The vomitoxin of offer quantitatively detects external member, quantitatively detects eight detection cards in external member and eight unions are contained in bottle, more
A vomitoxin quantitatively detects external member and is contained in again in Packaging box body.
It these are only the preferred embodiment of the utility model, be not intended to limit the utility model, for this field
Technical staff for, various modifications and changes may be made to the present invention.Within the spirit and principle of the utility model,
Any modifications, equivalent replacements and improvements are made should be included within the scope of protection of this utility model.
Claims (10)
1. a kind of vomitoxin quantitatively detects external member, which is characterized in that it includes eight unions and eight detection cards;
Eight union has eight tube bodies being connected with each other and the pipe cap with the opening cooperation of the tube body, each tube body
Internal storage have lyophilized in powdered time-resolved fluorescence microballoon, the time-resolved fluorescence microballoon includes detection microballoon
With Quality Control microballoon, the detection microballoon is the fluorescent microsphere of vomitoxin antibody mark, and the Quality Control microballoon is biotin labeling
Fluorescent microsphere;
The detection card includes bottom plate, the nitrocellulose filter being arranged on bottom plate and is overlapped on the nitrocellulose respectively
The water absorption pad and sample pad at film both ends are provided with the nature controlling line of coating Avidin and coating vomiting poison on the nitrocellulose filter
The detection line of plain antibody;
The circumferencial direction of the inner wall of the tube body along the tube body extends and the convex annular prominent to the center of circle direction of the tube body
Edge, is provided with diaphragm seal in the annular flange, the circumferencial direction and the annular of the periphery of the diaphragm seal along the tube body
Flange connects, the diaphragm seal, the tube body and the bottom wall of the inner wall below the annular flange and the tube body
For accommodating the accommodating chamber of time-resolved fluorescence microballoon, the time-resolved fluorescence microballoon is placed in the accommodating chamber composition.
2. vomitoxin according to claim 1 quantitatively detects external member, which is characterized in that the diaphragm seal is close to the pipe
Easy tear strip is provided on the surface of the opening of body, one end of the easy tear strip is fixedly connected with the diaphragm seal, described easy
The other end of tear strip extends to the opening direction of the tube body.
3. vomitoxin according to claim 2 quantitatively detects external member, which is characterized in that the diaphragm seal and the annular
The upper surface of flange is connected by viscous glutinous agent, and the easy tear strip corresponds to the close institute of the annular flange with the diaphragm seal
The position for stating the inner wall of tube body is fixedly connected.
4. the vomitoxin according to Claims 2 or 3 quantitatively detects external member, which is characterized in that the length of the easy tear strip
It spends L and is more than the annular flange and the height H at the edge of the opening.
5. vomitoxin according to claim 4 quantitatively detects external member, which is characterized in that is provided with and stretches on the pipe cap
The sealing-plug of the tubular body, the sealing-plug are hemispherical.
6. external member is quantitatively detected according to claim 1-3 any one of them vomitoxins, which is characterized in that set on the pipe cap
The sealing-plug for stretching into the tubular body is equipped with, the sealing-plug is hemispherical.
7. external member is quantitatively detected according to claim 1-3 any one of them vomitoxins, which is characterized in that the bottom plate is modeling
Expect bottom plate.
8. external member is quantitatively detected according to claim 1-3 any one of them vomitoxins, which is characterized in that the external member is also wrapped
The bottle with bottle cap is included, eight detection cards and eight union are placed in the bottle.
9. a kind of vomitoxin immue quantitative detection reagent box, which is characterized in that it includes, and multiple claim 1-8 are any described to be vomitted
It spits toxin and quantitatively detects external member.
10. vomitoxin immue quantitative detection reagent box according to claim 9, which is characterized in that the vomitoxin quantifies
Detection kit further includes Packaging box body, and multiple vomitoxins quantitatively detect external member and are contained in the Packaging box body.
Priority Applications (1)
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CN201721094727.6U CN207380052U (en) | 2017-08-29 | 2017-08-29 | A kind of vomitoxin quantitatively detects external member and kit |
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CN201721094727.6U CN207380052U (en) | 2017-08-29 | 2017-08-29 | A kind of vomitoxin quantitatively detects external member and kit |
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