CN207294796U - A kind of hbv nucleic acid quantitative Diagnosis kit - Google Patents

Abstract

The utility model discloses a kind of hbv nucleic acid quantitative Diagnosis kit, including nucleic acid extraction kit and nucleic acid amplification kit, the nucleic acid extraction kit includes the first sterilizing test tubes body for being marked with surfactant；It is marked with the second sterilizing test tubes body of the buffer solution for adjusting pH；It is marked with the 3rd sterilizing test tubes body of lysozyme or protease；It is marked with the 4th sterilizing test tubes body of phenol, chloroform and isoamyl alcohol mixed liquor；It is marked with the 4th sterilizing test tubes body of superparamagnetism silica nanometer magnetic bead liquid；It is marked with the 5th sterilizing test tubes body of the rinsing liquid that desalts；The first sterilizing test tubes body to the 5th sterilizing test tubes body is mounted on tube body laying rack；The tube body laying rack is fixed on interior constant temperature box body；The hbv nucleic acid quantitative Diagnosis kit of the utility model, step is simple, between each sterilizing test tubes body will not cross-infection, in addition, it can extract nucleic acid well；When needing tyre, directly semiconductor temperature-control device is loaded, it is easy to use.

Description

A kind of hbv nucleic acid quantitative Diagnosis kit

Technical field

It the utility model is related to a kind of detection kit, and in particular to a kind of hbv nucleic acid quantitative Diagnosis reagent Box, belongs to detection kit technical field.

Background technology

Hepatitis B is still one of public health problem of global most serious, and the cause of the death of 10 diseases, hepatitis B account for before the whole world 7th.Show according to the data of the World Health Organization, the population in the whole world about 30%, i.e. 2,000,000,000 people have hepatitis b virus infection Serological evidence, wherein 3.5 hundred million people are chronic infection, every year at least 1,000,000 people die of hepatic failure caused by HBV infection, Hepatic sclerosis and hepatocellular carcinoma；And China is universally acknowledged hepatitis B big country, the hepatitis carrier in China is up to 100,000,002 Million people, chronic hepatitis B hepatitis have 20,000,000, annual therefore dead number nearly 500,000；Prevention for hepatitis B and Diagnosis and treatment are the cardinal tasks of national infectious disease control；At present, domestic existing a variety of hepatitis B based on fluorescent quantitative PCR technique quantify Detection kit is applied in clinical detection, and the hepatitis B nucleic acid extracting method that these kits are provided is mainly the glycogen precipitation method With centrifugal column method；Such kit have the advantages that it is certain, still, there are many deficiencies part；As although the glycogen precipitation method are grasped Make comparisons simple, but can not effectively remove the PCR amplification in complex samples (such as hyperlipidemia, high bilirubin, haemolysis equal samples) Mortifier, is easy to cause appearance detection false negative, causes the missing inspection of sample；Although centrifugal column method is needed without high speed centrifugation Centrifuge tube is frequently replaced, it is cumbersome with duration, it is easy to cause nucleic acid " aerosol " pollution；For this reason, Chinese Patent Application No.： 201310689663.4 disclose a kind of hepatitis type B virus being applied in biomedical clinical diagnostic field（HBV）Nucleic acid Immue quantitative detection reagent box, kit include paramagnetic particle method nucleic acid extraction kit and HBV nucleic acid amplification kits, wherein paramagnetic particle method core Sour extracts kit includes cracking with reference to liquid, rinsing liquid, eluent, magnetic bead liquid；HBV nucleic acid amplification kits include HBV-PCR Reaction solution, enzyme mixation, HBV- internal standards, HBV qualitative reference product 1-4, negative quality-control product, critical positive quality control product, strong positive matter Control product；The invention it is easy to operate it is quick, of low cost, detection sensitivity is high, reproducible, primer and probe conservative is high, special Hypotype different in nature strong, covering hepatitis type B virus is different or mutation, the accuracy for being conducive to improve hepatitis B detection and specificity, draw The problems such as entering mark system in efficient, solve target gene and internal standard while mutually suppressing and disturb caused by expanding, Neng Gougao The whole PCR amplification whole process of monitoring of effect, avoid the occurrence of false negative result；But it is at hbv nucleic acid early period It is general to manage effect, it is not accurate enough to be easy to cause nucleic acid amount to obtain.

Utility model content

To solve the above problems, the utility model proposes a kind of hbv nucleic acid quantitative Diagnosis kit, step It is rapid simple, between each sterilizing test tubes body will not cross-infection, in addition, it can extract nucleic acid well；When needing tyre, directly Connect and load semiconductor temperature-control device, it is easy to use.

The hbv nucleic acid quantitative Diagnosis kit of the utility model, including nucleic acid extraction kit and nucleic acid expand Increase kit, the nucleic acid extraction kit includes the first sterilizing test tubes body for being marked with surfactant；It is marked with and adjusts the slow of pH Second sterilizing test tubes body of fliud flushing；It is marked with the 3rd sterilizing test tubes body of lysozyme or protease；Superparamagnetism silica is marked with to receive 4th sterilizing test tubes body of rice magnetic bead liquid；It is marked with the 5th sterilizing test tubes body of the rinsing liquid that desalts；The first sterilizing test tubes body is extremely 5th sterilizing test tubes body is mounted on tube body laying rack；The tube body laying rack is fixed on interior constant temperature box body；The interior constant temperature box External side bottom is provided with the temperature control groove inwardly concaved；Semiconductor temperature-control device is movably set with the inside of the temperature control groove；Institute State interior constant temperature box portion outside and be provided with outer packing babinet；The interior constant temperature box body top surface has been made into integration seal cover board, described close Capping plate on be provided with test tube body it is embedding go out straight slot；The buffer solution of pH is adjusted by the second sterilizing test tubes body；Adjust pH environment；And Pass through the surfactant of side in the first sterilizing test tubes body；Under certain pH environment, surfactant can split virocyte Solution, protein and polysaccharide precipitation, some metal ion chelation agents (EDTA etc.) in buffer solution can be chelated to nuclease institute Necessary metal ions M g2+, Ca2+, so as to suppress the activity of nuclease, protects nucleic acid not to be degraded；And pass through the 3rd sterilizing The lysozyme of side or protease promote cell rupture in test tube body；Meanwhile during nucleic acid extraction, by adding appropriate enzyme Make unwanted mass degradation, in favor of the isolation and purification of nucleic acid；Adding protease such as in lysate can be with protein degradation Matter, inactivates nuclease；Directly carried out finally by the superparamagnetism silica nanometer magnetic bead liquid of side in the 4th sterilizing test tubes body pure Change, complete after purification, to be rinsed by the rinsing liquid that desalts of side in the 5th sterilizing test tubes body；Magnetic is can obtain after completing rinsing Pearl mixtures of nucleic acids.

As preferred embodiment, side bottom is provided with tube socket in the interior constant temperature box body.

As preferred embodiment, the tube socket bottom surface is seal shape.

As preferred embodiment, the outer packing babinet takes a mouthful carton for lower hollow.

As preferred embodiment, described its power cord of semiconductor temperature-control device by interior constant temperature box body and outer packing babinet it Between interlayer introduce.

As preferred embodiment, its power cord input terminal of the semiconductor temperature-control device is electrically connected storage battery or adaptation Device.

Compared with prior art, the hbv nucleic acid quantitative Diagnosis reagent of the utility model Box, step is simple, between each sterilizing test tubes body will not cross-infection, in addition, it can extract nucleic acid well；When need in addition When, directly semiconductor temperature-control device is loaded, it is easy to use.

Brief description of the drawings

Fig. 1 is the overall structure diagram of the utility model.

Embodiment

Embodiment 1：

Hbv nucleic acid quantitative Diagnosis kit as shown in Figure 1, including nucleic acid extraction kit and nucleic acid expand Increase kit 1, the nucleic acid extraction kit includes the first sterilizing test tubes body 2 for being marked with surfactant；It is marked with and adjusts pH's Second sterilizing test tubes body 3 of buffer solution；It is marked with the 3rd sterilizing test tubes body 4 of lysozyme or protease；It is marked with superparamagnetism oxidation 4th sterilizing test tubes body 5 of silicon nanometer magnetic bead liquid；It is marked with the 5th sterilizing test tubes body 6 of the rinsing liquid that desalts；The first sterilizing examination 2 to the 5th sterilizing test tubes body 6 of tube body is mounted on tube body laying rack 7；The tube body laying rack 7 is fixed on interior constant temperature box body 8；Institute State interior 8 exterior bottom of constant temperature box body and be provided with the temperature control groove 9 inwardly concaved；The inner side of temperature control groove 9 is movably set with half Conductor temperature controller 10；Outer packing babinet 11 is provided with outside the interior constant temperature box body 10；Interior 10 top surface of the constant temperature box body one Be formed with seal cover board 12, be provided with the seal cover board 12 test tube body it is embedding go out straight slot 13；Pass through the second sterilizing test tubes body Adjust the buffer solution of pH；Adjust pH environment；And pass through the surfactant of side in the first sterilizing test tubes body；In certain pH environment Under, surfactant can make virocyte cracking, protein and polysaccharide precipitation, some metal ion chelation agents in buffer solution (EDTA etc.) can be chelated to metal ions M g2+, Ca2+ necessary to nuclease, so as to suppress the activity of nuclease, protected Nucleic acid is not degraded；And cell rupture is promoted by the lysozyme or protease of side in the 3rd sterilizing test tubes body；Meanwhile in nucleic acid In extraction process, make unwanted mass degradation by adding appropriate enzyme, in favor of the isolation and purification of nucleic acid；Such as cracking Protease is added in liquid to inactivate nuclease with protein degradation matter；Finally by the superparamagnetism of side in the 4th sterilizing test tubes body Silica nanometer magnetic bead liquid is directly purified, complete after purification, by the 5th sterilizing test tubes body side desalt rinsing liquid into Row rinsing；Magnetic bead mixtures of nucleic acids is can obtain after completing rinsing.

In another embodiment, interior 8 inside bottom of constant temperature box body is provided with tube socket 14.

In another embodiment, 14 bottom surface of tube socket is seal shape.

In another embodiment, the outer packing babinet 11 takes a mouthful carton for lower hollow.

In another embodiment, described its power cord of semiconductor temperature-control device 10 is by between interior constant temperature box body and outer packing babinet Interlayer introduces.

In another embodiment, its power cord input terminal of the semiconductor temperature-control device 10 is electrically connected storage battery or adapter.

Above-described embodiment, is only the better embodiment of the utility model, therefore all according to the utility model patent application range The equivalent change or modification that construction, feature and the principle is done, is included in the utility model patent application range.

Claims (6)

1. a kind of hbv nucleic acid quantitative Diagnosis kit, including nucleic acid extraction kit and nucleic acid amplification kit, It is characterized in that：The nucleic acid extraction kit includes the first sterilizing test tubes body for being marked with surfactant；It is marked with and adjusts pH's Second sterilizing test tubes body of buffer solution；It is marked with the 3rd sterilizing test tubes body of lysozyme or protease；It is marked with phenol, chloroform and isoamyl alcohol 4th sterilizing test tubes body of mixed liquor；It is marked with the 4th sterilizing test tubes body of superparamagnetism silica nanometer magnetic bead liquid；It is marked with and desalts 5th sterilizing test tubes body of rinsing liquid；The first sterilizing test tubes body to the 5th sterilizing test tubes body is mounted on tube body laying rack； The tube body laying rack is fixed on interior constant temperature box body；It is recessed that the external side bottom of interior constant temperature box is provided with the temperature control inwardly concaved Groove；Semiconductor temperature-control device is movably set with the inside of the temperature control groove；The interior constant temperature box portion outside is provided with outer packing babinet； The interior constant temperature box body top surface has been made into integration seal cover board, be provided with the seal cover board test tube body it is embedding go out straight slot.

2. hbv nucleic acid quantitative Diagnosis kit according to claim 1, it is characterised in that：The interior constant temperature Box body inside bottom is provided with tube socket.

3. hbv nucleic acid quantitative Diagnosis kit according to claim 2, it is characterised in that：The tube socket bottom Face is seal shape.

4. hbv nucleic acid quantitative Diagnosis kit according to claim 1, it is characterised in that：The outer packing Babinet takes a mouthful carton for lower hollow.

5. hbv nucleic acid quantitative Diagnosis kit according to claim 1, it is characterised in that：The semiconductor Its power cord of temperature controller is introduced by the interlayer between interior constant temperature box body and outer packing babinet.

6. hbv nucleic acid quantitative Diagnosis kit according to claim 5, it is characterised in that：The semiconductor Its power cord input terminal of temperature controller is electrically connected storage battery or adapter.