CN207227441U - A kind of chip type digitizes gene intelligent analysis system - Google Patents
A kind of chip type digitizes gene intelligent analysis system Download PDFInfo
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- CN207227441U CN207227441U CN201720559535.1U CN201720559535U CN207227441U CN 207227441 U CN207227441 U CN 207227441U CN 201720559535 U CN201720559535 U CN 201720559535U CN 207227441 U CN207227441 U CN 207227441U
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Abstract
The utility model is suitable for genetic test field, there is provided a kind of chip type digitizes gene intelligent analysis system, which includes：Electrochemistry gene chip, electrochemica biological sensor, controller and processor, electrochemica biological sensor, for detecting Electrochemistry gene chip in the front and rear electrochemical signals change of hybridization；Controller is used to control detection process of the electrochemica biological sensor to Electrochemistry gene chip, and the analog signal that electrochemica biological sensor is detected is converted into digital signal, is sent to processor and is analyzed；Processor is used to be analyzed according to the digital signal that controller transmits, and sends the real-time adjustment control device of instruction；The utility model embodiment is by detecting conversion of the Electrochemistry gene chip in the front and rear electrochemical signals of hybridization, you can accurately examines DNA, DNA inspections are carried out relative to fluorescent marker, testing cost is low, without carrying out fluorescent marker to sample, operating procedure is reduced, accelerates detection speed.
Description
Technical field
The utility model belongs to genetic test field, more particularly to a kind of intelligent analysis system of chip type digitlization gene point.
Background technology
Genetic chip, also known as DNA chip or biochip, the sequencing principle of genetic chip is to be based on sequencing by hybridization method,
Method i.e. by carrying out nucleic acid sequencing with the nucleic acid probe of one group of known array, known target is secured in one piece of substrate surface
Nucleotide probe, when the nucleic acid probe of correspondence position on the nucleotide sequence that fluorescent marker is carried in solution, genetic chip produces
During complementation matching, the probe location most strong by determining fluorescence intensity, the probe sequence of one group of sequence complete complementary of acquisition, accordingly
The sequence of target nucleic acid can be recombinated out.
Existing all gene detection systems realize gene sequencing by fluorescent marker mostly, and real based on fluorescence mark mark
Existing gene sequencing is therefore, with high costs, it is necessary to Fluorescence Scanner carries out the genetic chip after hybridization fluorescence signal acquisition, this
Outside, before being hybridized, it is also necessary to fluorescent marker is carried out to sample, the operation of fluorescent marker process is not only professional and cumbersome.
Utility model content
The utility model embodiment provides a kind of chip type digitlization gene intelligent analysis system, it is intended to which solution is based on fluorescence
Mark mark realize gene sequencing, it is necessary to Fluorescence Scanner to after hybridization genetic chip carry out fluorescence signal acquisition, it is with high costs,
The problem of operation of fluorescent marker process is not only professional and cumbersome.
The utility model is realized in this way a kind of chip type digitlization gene intelligent analysis system, the system comprises：
The system includes:Electrochemistry gene chip, electrochemica biological sensor, controller and processor, electrochemica biological
Sensor is connected with Electrochemistry gene chip and controller respectively, and controller is connected with processor,
Electrochemica biological sensor, for detecting Electrochemistry gene chip in the front and rear electrochemical signals change of hybridization；Control
Device processed is used to control detection process of the electrochemica biological sensor to Electrochemistry gene chip, and electrochemica biological sensor is examined
The analog signal measured is converted into digital signal, is sent to processor and is analyzed；Processor is used to be transmitted according to controller
The digital signal come is analyzed, and sends the real-time adjustment control device of instruction；
The utility model embodiment detects Electrochemistry gene chip in the front and rear electricity of hybridization by electrochemica biological sensor
The conversion of chemical signal, you can accurately examine DNA, carry out DNA inspections relative to fluorescent marker, testing cost is low, and need not
Fluorescent marker is carried out to sample, operating procedure is reduced, detection speed is accelerated, in addition, being carried out by electrochemica biological sensor
Detection also there is high sensitivity, it is pollution-free, it is reproducible the advantages that.
Brief description of the drawings
Fig. 1 is the structure diagram for the chip type digitlization gene intelligent analysis system that the utility model embodiment provides；
1. Electrochemistry gene chip, 2. electrochemica biological sensors, 3. controllers, 4. processors, 5. chip heating units,
6. temperature sensor.
Embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, is further elaborated the utility model.It should be appreciated that specific embodiment described herein is only explaining
The utility model, is not used to limit the utility model.
Fig. 1 is the structure diagram that the chip type that the utility model embodiment provides digitizes gene intelligent analysis system,
For convenience of description, only show and the relevant part of the utility model embodiment.
The system includes:Electrochemistry gene chip 1, electrochemica biological sensor 2, controller 3 and processor 4, electrochemistry
Biology sensor 2 is connected with Electrochemistry gene chip 1 and controller 2 respectively, and controller 3 is connected with processor 4,
Electrochemica biological sensor 2, for detecting Electrochemistry gene chip 1 in the front and rear electrochemical signals change of hybridization；
Controller 3 is used to control detection process of the electrochemica biological sensor 2 to Electrochemistry gene chip 1, and electrochemica biological is passed
The analog signal that sensor 2 detects is converted into digital signal, is sent to processor 4 and is analyzed；Processor 4 is used for according to control
The digital signal that device 3 is sent is analyzed, and sends the real-time adjustment control device 3 of instruction；
It is in hybridization front and rear that the utility model embodiment by electrochemica biological sensor 2 detects Electrochemistry gene chip 1
The conversion of electrochemical signals, you can accurately examine DNA, carry out DNA inspections relative to fluorescent marker, testing cost is low, and nothing
Need to sample carry out fluorescent marker, reduce operating procedure, accelerate detection speed, in addition, by electrochemica biological sensor 2 into
Row detection also there is high sensitivity, it is pollution-free, it is reproducible the advantages that.
In the utility model embodiment, it is by hybridizing the detection to carry out gene, needs stringent control in crossover process
Temperature processed, therefore, chip type digitlization gene intelligent analysis system further include：
Chip heating unit 5 and temperature sensor 6, chip heating unit 5 respectively with Electrochemistry gene chip 1 and the place
Reason device 4 connects, and temperature sensor 6 is connected with processor 4,
Chip heating unit 5 is used to heat Electrochemistry gene chip 1；
Temperature sensor 6 is used for the temperature for sensing Electrochemistry gene chip 1 in real time, and processor 4 is used for according to temperature sensing
The temperature value that device 6 senses judges whether driving chip heating unit 5.
In the utility model embodiment, Electrochemistry gene chip 1 includes：Chip substrate, using mask spraying plating in chip
The circuit of electrode and each electrode of connection on substrate and the DNA probe with hybridization indicator being fixed on the electrode；
In the utility model embodiment, chip substrate is silicon chip, glass substrate or ceramic chip, by taking silicon chip as an example
Illustrate, on silicon chip after the cleaning, the spraying plating of electrode and circuit (being used to connect each electrode) is carried out using mask, then
By added with the DNA probe of hybridization indicator fix on the electrode, in order to enable electrode and circuit it is more firm be fixed on silicon chip
On, on silicon chip after the cleaning, the spraying plating of adhesion layer is carried out first with mask, the spray of electrode and circuit is carried out on adhesion layer
Plating, will finally fix on the electrode added with the DNA probe of hybridization indicator.
In the utility model embodiment, hybridization indicator refers to the electroactive material that may indicate that hybridization information, such as：Two cyclopentadienyls
Iron and its derivative or methylene blue are common embedded hybridization indicator.
As the preferred embodiment of the utility model, gold is electrode and the optimal depositing materials of circuit, and titanium is adhesion layer
Optimal material, DNA probe end, which is embedded between sulfydryl, with gold electrode, forms Au-S keys, can be put with self assembly to electrode surface.
The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this
All any modification, equivalent and improvement made within the spirit and principle of utility model etc., should be included in the utility model
Protection domain within.
Claims (7)
1. a kind of chip type digitizes gene intelligent analysis system, it is characterised in that the system comprises：Electrochemical gene core
Piece, electrochemica biological sensor, controller and processor, the electrochemica biological sensor respectively with the electrochemical gene core
Piece and controller connection, the controller are connected with the processor,
The electrochemica biological sensor, for detecting Electrochemistry gene chip in the front and rear electrochemical signals change of hybridization；
The controller is used to control detection process of the electrochemica biological sensor to Electrochemistry gene chip, and by described in
The analog signal that electrochemica biological sensor detects is converted into digital signal, is sent to the processor and is analyzed；
The processor is used to be analyzed according to the digital signal that the controller transmits, and sends described in instruction adjusting in real time
Controller.
2. chip type as claimed in claim 1 digitizes gene intelligent analysis system, it is characterised in that the system is also wrapped
Include：
Chip heating unit and temperature sensor, the chip heating unit respectively with the Electrochemistry gene chip and the place
Device connection is managed, the temperature sensor is connected with the processor,
The chip heating unit is used to heat Electrochemistry gene chip；
The temperature sensor is used for the temperature for sensing Electrochemistry gene chip in real time, and processor is used for according to temperature sensor sense
The temperature value answered judges whether driving chip heating unit.
3. chip type as claimed in claim 1 or 2 digitizes gene intelligent analysis system, it is characterised in that the electrochemistry
Genetic chip includes：
Chip substrate, using electrode of the mask spraying plating on chip substrate and each electrode of connection circuit and be fixed on the electricity
The DNA probe with hybridization indicator on extremely.
4. chip type as claimed in claim 3 digitizes gene intelligent analysis system, it is characterised in that the electrochemical gene
Chip further includes：Using the adhesion layer of mask spraying plating on the chip substrate, the adhesion layer is located at the chip base
Between piece and the electrode and circuit.
5. chip type as claimed in claim 4 digitizes gene intelligent analysis system, it is characterised in that the electrode is gold,
The adhesion layer is titanium.
6. chip type as claimed in claim 5 digitizes gene intelligent analysis system, it is characterised in that the DNA probe is
By forming Au-S keys between the sulfydryl and gold electrode of probe molecule end, self assembly is put to electrode surface.
7. chip type as claimed in claim 3 digitizes gene intelligent analysis system, it is characterised in that the hybridization indicator
For Ferrocene and its derivative or methylene blue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720559535.1U CN207227441U (en) | 2017-05-22 | 2017-05-22 | A kind of chip type digitizes gene intelligent analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720559535.1U CN207227441U (en) | 2017-05-22 | 2017-05-22 | A kind of chip type digitizes gene intelligent analysis system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207227441U true CN207227441U (en) | 2018-04-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720559535.1U Expired - Fee Related CN207227441U (en) | 2017-05-22 | 2017-05-22 | A kind of chip type digitizes gene intelligent analysis system |
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| Country | Link |
|---|---|
| CN (1) | CN207227441U (en) |
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2017
- 2017-05-22 CN CN201720559535.1U patent/CN207227441U/en not_active Expired - Fee Related
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| CF01 | Termination of patent right due to non-payment of annual fee |