CN207181240U - For the Clonal diagnostic kit of flow cytometry mesoplasmatocyte - Google Patents
For the Clonal diagnostic kit of flow cytometry mesoplasmatocyte Download PDFInfo
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- CN207181240U CN207181240U CN201721119979.XU CN201721119979U CN207181240U CN 207181240 U CN207181240 U CN 207181240U CN 201721119979 U CN201721119979 U CN 201721119979U CN 207181240 U CN207181240 U CN 207181240U
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- box body
- groove
- clonal
- mesoplasmatocyte
- flow cytometry
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Abstract
The utility model provides one kind and is used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, and kit comprises at least：Kit includes box body,The top surface of box body is openable lid,Box body sets refrigeration groove,Refrigerate groove and bracket plate is set,Some grooves are set on bracket plate,Bracket plate is just covered on refrigeration groove,Refrigerate and insulation material is set in groove,One side of lower box body sets opening to be used to extract out refrigeration groove,Box body includes four sides and top surface,One of side sets openable window,Mirror is set on side corresponding thereto,Lighting unit is set in box body,Lighting unit includes power supply,Illuminating lamp and switch,The closure of window control switch,Illuminating lamp is arranged on the top surface of box body,Reagent in kit comprises at least FMC 7,CD19,CD20,CD22,CD27,CD28,CD34,CD38,CD56,CD117,CD138,cKappa,cLambda,CD45,HLA‑DR,Reagent is placed in groove.The utility model is easy to use, and light-shading effect is good.
Description
Technical field
It the utility model is related to one kind and be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte.
Background technology
Thick liquid cell (plasma cell), also known as effect B cell, it is that the thin of final function phases is arrived in bone-marrow-derived lymphocyte development
Born of the same parents.It is immunoglobulin (immunoglobulin) function that thick liquid cell, which has synthesis, storage antibody, and it is anti-to participate in humoral immunity
Should, it plays irreplaceable effect during humoral immunity.Huppert's disease (multiple myeloma) and slurry
Chronic myeloid leukemia is a kind of the malignant plasma cell dyscrasia, and its tumour cell originates from the thick liquid cell in marrow, thick liquid cell it is Clonal
It is then to judge its whether abnormal critical index.
Wood published an article in 2007 and pointed out, in thick liquid cell, CD38 strongly expresseds, determined that thick liquid cell occupies by the antibody
The ratio of nucleus；Thick liquid cell is differentiated by B cell, and CD38+CD19+/CD38CD19- cells are divided according to CD38-CD19,
Further confirm that whether two groups of cells are abnormal；According to CD38+CD19+/CD38+CD19- groups of cells, it is detected respectively
CKappa/cLambda expressions, it is abnormal if monoclonal.According to antigen groups such as CD56, CD19, CD20, CD27, CD28
Close, be advantageous to the confirmation of the immunophenotype of MM malignant plasma cells, if CD56+, poor prognosis；CD28+, then plasma cell proliferation ability
By force, CD27 expression is increased, and can activate NF-KB, strengthens tumour cell Anti-G value；The high expression of CD20, CD56, CD117, HLA-
DR low expressions help to differentiate plasma cell leukemia and Huppert's disease etc..
Flow cytometry (Flow Cytometry, FCM) is a kind of cell to defiled in liquid stream or other biologies
Particulate (such as microballoon, bacterium, small scale mode biology etc.) carries out the technology of fast quantitative analysis and sorting one by one.Quickly determine single
The biological property of cell or organelle.Be characterized in by quickly determine Kurt resistance, fluorescence, light scattering and light absorbs come
It is many important to quantitative determine cell DNA content, cell volume, protein content, enzymatic activity, cell-membrane receptor and surface antigen etc.
Parameter.Cell of different nature is separated according to these parameters, to obtain for biology and the pure cell colony of medical research.
Highest separation velocity has reached 30,000 cells each second at present.Therefore, thick liquid cell can be examined using flow cytometry
Survey.
At present dedicated for the Clonal diagnostic kit of thick liquid cell, the problem of often existing is that shaded effect is bad, and
The statistics to antibody surplus can not be realized.
Utility model content
In view of the above the shortcomings that prior art, the purpose of this utility model is that providing one kind is used for flow cytometry
The Clonal diagnostic kit of mesoplasmatocyte, for solving deficiency of the prior art.
In order to achieve the above objects and other related objects, the utility model offer one kind is used for flow cytometry mesoplasmatocyte
Clonal diagnostic kit, the kit include box body, and the top surface of box body is openable lid, is set in the box body cold
Groove is hidden, the refrigeration groove sets pop-up frid, sets some grooves on the bracket plate, the bracket plate is just covered in refrigeration
On groove, insulation material is set, and a side of the box body sets opening to be used to extract out refrigeration groove, described in the refrigeration groove
Box body includes four sides and top surface, and one of side sets openable window, mirror is set on side corresponding thereto
Son, the box body is interior to set lighting unit, and the lighting unit includes power supply, illuminating lamp and switch, the power supply, illuminating lamp
And switch surrounds closed-loop path, the closure of the window control switch, the illuminating lamp is arranged on the top surface of box body, the examination
Reagent in agent box comprise at least FMC-7, CD19, CD20, CD22, CD27, CD28, CD34, CD38, CD56, CD117,
CD138, cKappa, cLambda, CD45, HLA-DR, the reagent are placed in groove.
Preferably, the refrigeration groove is regular hexahedron.
Preferably, the kit also includes piezo-resistance and control device, and at least one bottom portion of groove sets pressure-sensitive electricity
Resistance, the control device include display unit and control unit, described control unit connection display unit and piezo-resistance.
Preferably, the groove on the bracket plate is divided into 3 areas, and respectively antibody district, common agents area and antibody is anti-
Answer plate rest area, the groove in the antibody district is arranged in fan-shaped,
Preferably, the piezo-resistance is located at and held in CD56 groove.In general CD56 usage amount is less, only with one
It is secondary, therefore for the statistics of CD56 usage amounts, contribute to the statistics to whole kit usage amount.
Preferably, the cross section of the top surface is inverted V-arrangement.Form similar roof shape structure.
As described above, of the present utility model be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, have following
Beneficial effect：
Because there is provided window and mirror so that without opening kit with regard to the situation of reagent can be observed so that whole
The light-shading effect of kit is preferable, while inside is provided with illuminating lamp, further such that observation becomes apparent from understanding；In kit
Reagent subregion place, therefore arrange rule, reagent should not be taken by mistake；Piezo-resistance, which to use every time, can all be recorded once,
So very easily know the amount of remaining reagent.
Brief description of the drawings
Fig. 1 is shown as box body structure schematic diagram.
Fig. 2 is shown as refrigerating groove schematic diagram.
Fig. 3 is shown as bracket plate structure schematic diagram.
Component label instructions
1 side
101 windows
102 mirrors
103 switches
104 openings
2 refrigeration grooves
3 bracket plates
301 antibody districts
3011 grooves
302 common agents areas
303 antibody response plate rest areas
Embodiment
Embodiment of the present utility model is illustrated by particular specific embodiment below, those skilled in the art can be by this
Content disclosed by specification understands other advantages and effect of the present utility model easily.
Fig. 1 is referred to Fig. 3.It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to
Coordinate the content disclosed in specification, so that those skilled in the art understands and reads, be not limited to the utility model
Enforceable qualifications, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or size
Adjustment, in the case where not influenceing the effect of the utility model can be generated and the purpose that can reach, all should still fall in the utility model
Disclosed technology contents are obtained in the range of covering.Meanwhile in this specification it is cited as " on ", " under ", " left side ",
The term on " right side ", " centre " and " one " etc., understanding for narration is merely convenient to, and it is enforceable to be not used to restriction the utility model
Scope, its relativeness are altered or modified, enforceable when being also considered as the utility model in the case where changing technology contents without essence
Category.
Such as Fig. 1~3, the utility model provides one kind and is used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, institute
Stating kit includes box body, and the box body includes bottom surface, 4 sides 1 and top surface.Two adjacent sides 1 are orthogonal.Top
The cross section in face is inverted V-arrangement and can opened, and forms the structure on similar roof.Therefore so that house is presented in whole box body
Shape, give tester a kind of sensation of family.The top of V-arrangement causes whole kit better heat preservation simultaneously.
The box body sets refrigeration groove 2, and refrigeration groove is hexahedron, and top surface is opening.Bracket plate is set on the refrigeration groove
3, bracket plate is just covered on refrigeration groove, and some grooves 3011 are set on the bracket plate, insulation is set in the refrigeration groove
Material, biological ice can be selected in the present embodiment, can be with multigelation.One side of the lower box body sets opening 104
For refrigeration groove to be extracted out.
One of side sets openable window 101, and mirror 102, the box body are set on side corresponding thereto
Interior setting lighting unit, the lighting unit include power supply, illuminating lamp and switch, and the power supply, illuminating lamp and switch enclose
Into closed-loop path, the closure of the controlling switch 103 of window 101, the illuminating lamp is arranged on the top surface of box body.In the present embodiment
Described in switch select proximity switch, the switch is arranged on side and is located near window, as long as therefore outwardly opening out
Close, you can the opening for completing illuminating lamp is convenient and swift.Now, mode user is clearly clearly visible that the reagent in kit,
It is convenient and swift without opening completely.
In another embodiment, the groove on the bracket plate is divided into 3 areas, respectively antibody district 301, common agents area
302 and antibody response plate rest area 303.Wherein antibody response area to be fan-shaped, be mainly used in FMC-7, CD19, CD20,
The reagents such as CD22, CD27, CD28, CD34, CD38, CD56, CD117, CD138, cKappa, cLambda, CD45, HLA-DR.More
Further, tri- kinds of passages of CD38 and cKappa/cLambda discharge around CD19BV421, and it is thin originating from B both to have highlighted thick liquid cell
Born of the same parents and cKappa/cLambda importance, simultaneously because CD19BV421 antibody bottles are small, it can further save space.Fan-shaped
Outer layer arrangement is according to antibody passage and pipe number successively annular array, and pipe number 1~3 is arranged to innermost layer from outermost layer successively, each
Passage facilitates operating personnel to take, and be not easy confusion according to the arrangement from left to right in below table.
The reagent distributing order of table 1
In another embodiment, the groove inner bottom part for placing CD56 sets piezo-resistance, the piezo-resistance connection control
Device, the control device include display unit and control unit, described control unit connection display unit and piezo-resistance, institute
It is display screen to state display unit, and control unit is single-chip microcomputer.The control device can be arranged on the outside of box body, pass through circuit
It is connected with the piezo-resistance of inside, control device can also be arranged on tray interior.CD56 amount of reagent described in general
It is smaller, therefore only with once.Therefore, often using a CD56 after, can all cause pressure change when placing reagent bottle, record uses
Once, final access times may be by display unit and show so that user knows the amount of remaining reagent in kit
And the amount of the reagent used.The control device is not related to the improvement of software program as just hardware unit.
Further, the antibody response plate rest area be except can place antibody response plate, for example, 96 hole reaction plates it
Suction pipe can also be placed outside, for aspirating reagent.
Further, the common agents area can place erythrocyte cracked liquid reagent, 10xPBS mother liquor reagents, distilled water
Reagent, rupture of membranes solution etc..
So the utility model effectively overcomes various shortcoming of the prior art and has high industrial utilization.
Above-described embodiment only illustrative principle of the present utility model and its effect are new not for this practicality is limited
Type.Any person skilled in the art can all be carried out without prejudice under spirit and scope of the present utility model to above-described embodiment
Modifications and changes.Therefore, such as those of ordinary skill in the art without departing from the essence disclosed in the utility model
God and all equivalent modifications completed under technological thought or change, should be covered by claim of the present utility model.
Claims (6)
1. one kind is used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that the kit at least wraps
Include：Box body, the top surface of box body is openable lid, and the box body sets refrigeration groove (2), and the refrigeration groove (2) sets pop-up
Frid (3), some grooves (3011) are set, the bracket plate (3) is just covered on refrigeration groove (2), institute on the bracket plate
State and insulation material is set in refrigeration groove, a side of the box body sets opening (104) to be used to extract out refrigeration groove, the box
Body includes four sides (1) and top surface, and one of side sets openable window (101), on side corresponding thereto
Mirror (102) is set, lighting unit is set in the box body, the lighting unit includes power supply, illuminating lamp and switch
(103), the power supply, illuminating lamp and switch surround closed-loop path, the closure of window (101) controlling switch, the photograph
Bright lamp is arranged on the top surface of box body, the reagent in the kit comprise at least FMC-7, CD19, CD20, CD22, CD27,
CD28, CD34, CD38, CD56, CD117, CD138, cKappa, cLambda, CD45, HLA-DR, the reagent are placed in groove
In.
2. according to claim 1 be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that：Institute
State hexahedron of the refrigeration groove (2) for rule.
3. according to claim 1 be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that：Institute
Stating kit also includes piezo-resistance and control device, and at least one bottom portion of groove sets piezo-resistance, the control device bag
Include display unit and control unit, described control unit connection display unit and piezo-resistance.
4. according to claim 1 be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that：Institute
State the groove on bracket plate and be divided into 3 areas, respectively antibody district (301), common agents area (302) and antibody response plate is placed
Area (303), the groove on the antibody district (301) are arranged in fan-shaped.
5. according to claim 3 be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that：Institute
Piezo-resistance is stated to be located at for holding in CD56 groove.
6. according to claim 1 be used for the Clonal diagnostic kit of flow cytometry mesoplasmatocyte, it is characterised in that：Institute
The cross section for stating top surface is inverted V-arrangement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721119979.XU CN207181240U (en) | 2017-09-01 | 2017-09-01 | For the Clonal diagnostic kit of flow cytometry mesoplasmatocyte |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721119979.XU CN207181240U (en) | 2017-09-01 | 2017-09-01 | For the Clonal diagnostic kit of flow cytometry mesoplasmatocyte |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207181240U true CN207181240U (en) | 2018-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721119979.XU Active CN207181240U (en) | 2017-09-01 | 2017-09-01 | For the Clonal diagnostic kit of flow cytometry mesoplasmatocyte |
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| CN (1) | CN207181240U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113419066A (en) * | 2021-08-23 | 2021-09-21 | 信纳克(北京)生化标志物检测医学研究有限责任公司 | Reagent composition for screening and/or diagnosing clonal diseases by one-step method and application thereof |
-
2017
- 2017-09-01 CN CN201721119979.XU patent/CN207181240U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113419066A (en) * | 2021-08-23 | 2021-09-21 | 信纳克(北京)生化标志物检测医学研究有限责任公司 | Reagent composition for screening and/or diagnosing clonal diseases by one-step method and application thereof |
| CN113419066B (en) * | 2021-08-23 | 2021-11-02 | 信纳克(北京)生化标志物检测医学研究有限责任公司 | Reagent composition for screening and/or diagnosing clonal diseases by one-step method and application thereof |
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