CN206956042U - A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum - Google Patents
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum Download PDFInfo
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- CN206956042U CN206956042U CN201720801494.2U CN201720801494U CN206956042U CN 206956042 U CN206956042 U CN 206956042U CN 201720801494 U CN201720801494 U CN 201720801494U CN 206956042 U CN206956042 U CN 206956042U
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Abstract
The technical field of the utility model category waste water control, it is related to a kind of preparation facilities of penicillin resistant class amonifying bacteria microbial inoculum, it includes water tank inlet, A/O reactors, second pond, and the A/O reactors are divided into four rooms of field word length cube shape be arrangeding in parallel, cell partitions are provided between each room；Agitator is provided with anoxic compartment, the first aerobic compartment, the second aerobic compartment, the 3rd aerobic compartment are respectively equipped with sand head aeration head and dissolved oxygen meter, using the utility model device, can effectively remove the organic nitrogen in waste water produced from penicillin production, sanitary sewage.Microbial inoculum rapid development simultaneously, obvious processing effect is adaptable, energy fast enriching, beneficial to the application in actual motion.And microbial inoculum oxygen demand during production application is low, aeration quantity is reduced, operating cost is saved, organic nitrogen removal efficiency high, there is great environmental benefit and economic benefit.
Description
Technical field
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum is the utility model is related to, it is applied in penicillins system
The application of denitrogenation processing in medicine waste water and municipal sewage, belong to the technical field to waste water control using microorganism.
Background technology
China is penicillin medicine big producer, and waste water produced from penicillin production, which is that the high concentration of a kind of difficult for biological degradation is nitrogenous, to be had
Machine waste water, the principal mode of nitrogen has organic nitrogen and ammoniacal nitrogen in waste water, and typically based on organic nitrogen, it mainly includes albumen
Matter, polypeptide, amino acid nucleic acids etc..Biological denitrificaion mainly includes three ammonification, nitrification and denitrification processes, and wherein ammonifying process is
Amonifying bacteria is converted into organic nitrogen the process of ammonia nitrogen, is the beginning of whole denitrification process, directly affects the denitrification effect in later stage；
Nitrifying process is by NH first by ammonia oxidation bacteria4 +- N is oxidized to NO2 -- N's, followed by by NOB by NO2 -- N oxygen
Turn to NO3 -- N nitrite nitrogen oxidation stage；Denitrification process is by caused NO3 -- N is under scarce/anaerobic condition by anti-
Nitrobacteria is converted into nitrogenous gas (nitrous oxide or nitrogen).Traditional biological denitrification process mainly includes nitrification and denitrification
Two stages, but containing macromolecular nitrogen substances such as a large amount of protein in pharmacy waste water, these materials are in traditional denitrification process
Difficulty is removed, water outlet total nitrogen concentration is higher；Organic nitrogen removal is inefficient simultaneously and sewage effluents total nitrogen is difficult to further
The principal element of reduction, these water outlets cause nutrients in rivers and lakes to increase, eutrophication occur.There is researcher to carry
Go out, organic nitrogen is controlled in below 3mg/L, can effectively control body eutrophication.Late nineteen nineties in last century, in environment
Antibiotic cause most attention in the world, as a result show antibiotic generally existing in different environments, including surface water, life
Sewage, underground water and drinking water, the presence of these antibiotic reduce the efficiency of denitrogenation flora, are surface water body eutrophication
One of major reason.
There is the research work in terms of the culture and microbial inoculum development of many document reports nitrification and denitrification bacterium.Such as：
Chinese invention patent (application number：200910058558.4) disclose a kind of autotrophy heterotrophism symbiosis ammonia oxidation for handling ammonia nitrogen waste water
Microbial inoculum and application thereof, the microbial inoculum are formed by mixed culture such as nitrosomonas, hot monad, pseudomonad, achromobacters；In
State's patent of invention (application number：200610020315.8) disclose a kind of preparation side for handling the microbial bacterial agent of ammonia nitrogen in waste water
Method and application thereof, the microbial inoculum are made up of 3 plants of heterotrophic ammonia-oxidizing bacterium；Chinese invention patent (application number：200610128300.3) public
Opened it is a kind of containing nitrobacteria, denitrifying bacteria, photosynthetic bacteria, polyP bacteria, bacillus, saccharomycete microorganism complex,
And the method using the complexing agent Treating Municipal Sewage, but the research of amonifying bacteria microbial inoculum is less.This microbial inoculum has efficient resistance to green grass or young crops
Mycin ammoniation and the decomposition efficiency that organic nitrogen can be significantly improved, while there is nitrification and denitrification performance.The microbial inoculum is used
In strengthening wastewater biological denitrificaion, accelerate the decomposition of algae in earth's surface eutrophication water, it is significant to reduce nitrate pollution.
The content of the invention
The utility model is the deficiency to solve the above problems, there is provided a kind of dress for preparing penicillin resistant class amonifying bacteria microbial inoculum
Put.The amonifying bacteria microbial inoculum can be solved under the conditions of containing penicillin in waste water, and traditional biological denitrogenation method is to machine nitrogen Ammoniation efficiency
The problem of not high, the microbial inoculum can greatly improve startup time and the treatment effeciency of nitrogenous effluent biological treatment.
The utility model is achieved using following technical scheme：
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum of the utility model, including water tank inlet, A/O reaction
Device, second pond, the A/O reactors are divided into four rooms of field word length cube shape be arrangeding in parallel, cell partitions are provided between each room,
Four rooms are respectively anoxic compartment, the first aerobic compartment, the second aerobic compartment, the 3rd aerobic compartment；Set in anoxic compartment
There is agitator, mixing arm area is 18cm2, the first aerobic compartment, the second aerobic compartment, set respectively in the 3rd aerobic compartment
There is a sand head aeration head, the first aerobic compartment, the second aerobic compartment, be respectively equipped with dissolved oxygen meter in the 3rd aerobic compartment；Sand
The internal diameter of head aeration head is 4cm.
The A/O reactors total measurement (volume) is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment has
Effect volume is 4L.
Anoxic compartment, the first aerobic compartment AB cell partitions and A/O reactor walls between tip position be provided with the length of side
For the AB cell partitions square upper ports of reactor wall length 1/10th, the top of AB cell partitions square upper ports goes out
Mouth is apart from top 10mm.
The first aerobic compartment, the second aerobic compartment BC cell partitions and A/O reactor walls between tip position be provided with
The length of side is the BC cell partitions square lower ports of reactor wall length 1/10th, under BC cell partitions square lower ports
Portion is exported apart from bottom 10mm.
The second aerobic compartment, the 3rd aerobic compartment CD cell partitions and A/O reactor walls between tip position be provided with
The length of side be reactor wall length 1/10th CD cell partitions square upper ports, CD cell partitions square upper ports it is upper
Portion outlet is located at top 10mm.
The water tank inlet top is provided with water inlet switch, and bottom is provided with effluent switch, and water tank inlet passes through effluent switch, entered
The water inlet switch of the anoxic compartment of water pump, water inlet pipe and A/O reactors is sequentially connected, and anoxic compartment side walls are additionally provided with return duct and opened
Close, carry out sludge reflux, be provided with agitator in anoxic compartment, the first aerobic compartment, the second aerobic compartment, in the 3rd aerobic compartment
It is respectively equipped with sand head aeration head, the first aerobic compartment, the second aerobic compartment, is respectively equipped with DO determination in the 3rd aerobic compartment
Instrument, the first aerobic compartment and the 3rd aerobic compartment are sequentially connected gas flowmeter and air pump by pipeline respectively；Anoxic compartment sides
The return duct switch of wall is connected by pipeline through the bottom of sludge reflux pump and second pond, and leads to sludge from final clarifier mouth；It is described
The outer wall of 3rd aerobic compartment is connected with A/O reactor outlet pipes, is connected through pipeline with the middle part of second pond side, second pond
Another upper lateral part is provided with secondary clarifier effluent pipe, the excess sludge of sludge from final clarifier mouth discharge, the penicillin resistant as produced in batches
Class amonifying bacteria microbial inoculum.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the second pond be top be two sections of external diameters not
Same, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth is located at the tapering bottom of cone.
The remarkable advantage that the utility model has compared with prior art is：
(1) the amonifying bacteria microbial inoculum prepared using the utility model device ammonia in the waste water of the 10mgL-1 containing penicillin
It is very high to change efficiency.Highest ammonification rate reaches as high as 30.5mg organic nitrogens/(g SSh) in 24h, and organic nitrogen degradation effect is excellent
It is good, far beyond the ammonification effect that microbial inoculum is reported in other documents.
(2) the amonifying bacteria microbial inoculum auxotype is heterotroph, grows rapidly, can quickly increase under the conditions of containing penicillin
Grow, it is possible to reduce initial cost and dosage.
(3) the utility model device making amonifying bacteria microbial inoculum cost is low, small investment, beneficial to a large amount of productions.
(4) the utility model device is applicable not only to the processing of the waste water containing penicillins, it may also be used for other class antibiotic
The improvement of dirty (useless) water containing organic nitrogen such as waste water, city domestic sewage, enriched surface water, has a extensive future, with compared with
High social benefit.
Brief description of the drawings
Fig. 1, the utility model apparatus structure schematic diagram.
Fig. 2 is the schematic diagram of four cell partitions of A/O reactors in the utility model device.
Embodiment
Specific embodiment of the present utility model is described in detail with reference to the accompanying drawings.
Referring to Fig. 1 to Fig. 2.
Each part numbers are in figure：
1. the water inlet switch 7. of 5. water inlet pipe of water inlet switch 2. water tank inlet, 3. effluent switch, 4. intake pump 6. flows back
Pipe switch 8.A/O reactor 10. air pumps of 9.A/O reactors outlet pipe 11. sand head aeration head, 12. agitators 13. 2 are heavy
The secondary clarifier effluent pipe 19 2 of pond 14. gas flowmeter, 15. dissolved oxygen meter, 16 sludge reflux pump, 17. return duct 18 is heavy
Pond mud discharging mouth.
Anoxic compartment a, the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d；
On AB cell partitions square upper port a1, BC cell partitions square lower port b1, CD cell partitions squares
Port c1.
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum, including water tank inlet 2, A/O reactors 8, second pond
13, four rooms of field word length cube shape that the A/O reactors 8 divide to be arranged in parallel are provided with cell partitions between each room, and described four
Individual room is respectively anoxic compartment a, the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d；It is provided with anoxic compartment a
Agitator 12, mixing arm area A=18cm2, interior point of the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d
Not She You sand head aeration head 11, the first aerobic compartment b, the second aerobic compartment c, be respectively equipped with dissolved oxygen in the 3rd aerobic compartment d
Analyzer (15)；The internal diameter of sand head aeration head 11 is 4cm.
The total measurement (volume) of A/O reactors 8 is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment has
Effect volume is 4L.
Anoxic compartment a, the first aerobic compartment b AB cell partitions and A/O reactor walls between tip position be provided with side
AB cell partitions square upper port a1, the AB cell partitions square upper ports a1's of a length of reactor wall length 1/10th
Upper outlet is apart from top 10mm.
The first aerobic compartment b, the second aerobic compartment c BC cell partitions and A/O reactor walls between tip position set
There are BC cell partitions square lower port b1, BC the cell partitions square lower ports that the length of side is reactor wall length 1/10th
B1 lower part outlet is apart from bottom 10mm.
Aerobic compartment c, aerobic compartment d CD cell partitions and A/O reactor walls between tip position be provided with the length of side
CD cell partitions square upper port c1, the CD cell partitions square upper ports c1 of reactor wall length 1/10th top
Outlet is located at top 10mm.
The top of water tank inlet 2 is provided with water inlet switch 1, and bottom is provided with effluent switch 3, and water tank inlet 2 is opened by water outlet
Close 3, intake pump 4, the anoxic compartment a water inlet switch 6 of water inlet pipe 5 and A/O reactors 8 is sequentially connected, anoxic compartment a side walls are also
Provided with return duct switch 7, carry out sludge reflux, be provided with agitator 12 in anoxic compartment a, the first aerobic compartment b, second it is aerobic every
It is respectively equipped with sand head aeration head 11 in room c, the 3rd aerobic compartment d, it is the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic
Dissolved oxygen meter 15 is respectively equipped with compartment d, and (Fig. 1 is structural representation, does not all show the first aerobic compartment b, second
Have sand head aeration head 11 and dissolved oxygen meter 15 in the aerobic aerobic compartment d of compartment c and the 3rd), the first aerobic compartment b and
Three aerobic compartment d are sequentially connected gas flowmeter 14 and air pump 10 by pipeline respectively；The return duct switch of anoxic compartment a side walls
7 are connected by pipeline through sludge reflux pump 16 with the bottom of second pond 13, and lead to sludge from final clarifier mouth 19；Described 3rd is aerobic
Compartment d outer wall is connected with A/O reactors outlet pipe 9, is connected through pipeline with the middle part of the side of second pond 13, second pond 13 it is another
One upper lateral part is provided with secondary clarifier effluent pipe 18, the excess sludge that sludge from final clarifier mouth 19 is discharged, the resistance to mould as produced in batches
Plain class amonifying bacteria microbial inoculum.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the second pond 13 be top be two sections of external diameters not
Same, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth 19 is located at the tapering bottom of cone.
The method that penicillin resistant class amonifying bacteria microbial inoculum is prepared using described device, is comprised the following steps：
A. amonifying bacteria enriched medium is prepared：Each material composition of amonifying bacteria enriched medium is：Peptone 5g, NaCl
0.25g, FeSO4·7H2O 0.01g, K2HPO40.5g, MgSO4·7H2O 0.5g, trace element solution 2ml, ultra-pure water 1L,
pH 7.2；Each material is sequentially placed into triangular flask, triangular flask is put into high-pressure sterilizing pot, sterilize 30min at 121 DEG C, obtains
It is standby to amonifying bacteria enriched medium；
The micro- solution mass, which forms, is：EDTA 0.4g, ZnSO4·7H2O 0.22g, CoCl
6H2O0.25g, MnCl20.25g, CuSO4·5H2O 0.1g, Na2MoO4·2H2O 0.15g, H3BO30.15g, ultra-pure water 1L；
B. the domestication culture of amonifying bacteria:Domestication culture for the first time, step a 100mL is added in 250mL shaking flask one
The amonifying bacteria enriched medium of sterilizing, then measure 50mL activated sludge (North China pharmacy waste water produced from penicillin production A/O handling process
Second pond activated sludge), it is added in shaking flask one, adds 0.01g penicillin, it is primary domestication liquid to obtain mixed liquor；At 25 DEG C,
Cultivate 24h under 150r/min oscillating conditions, shaking flask preferably oxygen condition, obtain first time sludge acclimatization bacterium solution；Second of domestication training
Support, take step a amonifying bacteria enriched medium 100mL to be fitted into shaking flask two again, take 50mL first time sludge acclimatization bacterium solutions
Supernatant be transferred in shaking flask two, cultivate 24h under 25 DEG C, 150r/min oscillating conditions, the preferably oxygen condition of shaking flask two, obtain
Second of sludge acclimatization bacterium solution；Third time domestication culture, take step a amonifying bacteria enriched medium 100mL to load again and shake
In bottle three, the supernatant of 50mL first time sludge acclimatization bacterium solutions is taken to be transferred in shaking flask three, in 25 DEG C, 150r/min oscillating conditions
Lower culture 24h, the preferably oxygen condition of shaking flask three, obtains third time sludge acclimatization bacterium solution；Domestication culture repeats five times, obtains the 5th time
Sludge acclimatization bacterium solution, as amonifying bacteria sludge acclimatization bacterium solution, its mixed liquor volatile suspended solid, MLVSS concentration (MLVSS) concentration are
4000mg/L。
C, microbial inoculum is prepared by A/O reactors 8；Four compartments add 200mL the 5th time ammonification in A/O reactors 8
Bacteria sludge tames bacterium solution, opens water inlet switch 6, and sewage enters anoxic compartment a, and agitator 12 is stirred, and sewage enters successively
Enter the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d, while open the return duct switch of anoxic compartment a side walls
7, sludge reflux is carried out, 75%, sewage passes sequentially through AB cell partitions square upper ports in the reactor for reflux ratio control
A1, BC cell partitions square lower port b1, CD cell partitions square upper port c1 flow to the 3rd aerobic compartment d rooms, port
Position it is different, prevent from forming short stream；Treated liquid is discharged into second pond 13 by A/O reactors outlet pipe 9, and supernatant leads to
Cross secondary clarifier effluent pipe 18 to discharge, excess sludge is discharged by sludge from final clarifier mouth 19, thin so as to which penicillin resistant class ammonification be made
Bacteria agent is penicillin resistant class amonifying bacteria microbial inoculum.
To sum up, the penicillin resistant amonifying bacteria microbial inoculum is gradual by enrichment, domestication, culture ammonification performance under aerobic conditions
Improve, can effectively remove the organic nitrogen in waste water produced from penicillin production, sanitary sewage.Microbial inoculum rapid development simultaneously, treatment effect
Significantly, it is adaptable, energy fast enriching, beneficial to the application in actual motion.And microbial inoculum is aerobic during production application
Measure it is low, reduce aeration quantity, save operating cost, organic nitrogen removal efficiency high, there is great environmental benefit and economic benefit.
Claims (4)
1. a kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, including water tank inlet (2), A/O reactors
(8), second pond (13), the A/O reactors (8) are divided into four rooms of field word length cube shape be arrangeding in parallel, are provided between each room
Cell partitions, four rooms are respectively anoxic compartment (a), the first aerobic compartment (b), the second aerobic compartment (c), the 3rd aerobic
Compartment (d)；Agitator (12) is provided with anoxic compartment (a)；It is first aerobic compartment (b), the second aerobic compartment (c), the 3rd aerobic
Sand head aeration head (11), the first aerobic compartment (b), the second aerobic compartment (c), the 3rd aerobic compartment are respectively equipped with compartment (d)
(d) dissolved oxygen meter (15) is respectively equipped with；
A/O reactors (8) total measurement (volume) is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment is effective
Volume is 4L；
Anoxic compartment (a), the first aerobic compartment (b) AB cell partitions and A/O reactor walls between tip position be provided with side
The AB cell partitions square upper ports (a1) of a length of reactor wall length 1/10th, AB cell partitions square upper ports
(a1) upper outlet is apart from top 10mm；
The first aerobic compartment (b), aerobic compartment (c) BC cell partitions and A/O reactor walls between tip position be provided with side
The BC cell partitions square lower ports (b1) of a length of reactor wall length 1/10th, BC cell partitions square lower ports
(b1) lower part outlet is apart from bottom 10mm；
The second aerobic compartment (c), the 3rd aerobic compartment (d) CD cell partitions and A/O reactor walls between tip position set
There are the CD cell partitions square upper ports (c1) that the length of side is reactor wall length 1/10th, CD cell partitions squares upper end
The upper outlet of mouth (c1) is located at top 10mm；
Water tank inlet (2) top is provided with water inlet switch 1, and bottom is provided with effluent switch (3), and water tank inlet (2) passes through water outlet
Switch (3), intake pump (4), the water inlet switch (6) of water inlet pipe (5) and the anoxic compartment (a) of A/O reactors (8) are sequentially connected,
Anoxic compartment (a) side wall is additionally provided with return duct switch (7), carries out sludge reflux, agitator (12) is provided with anoxic compartment (a),
First aerobic compartment (b), the second aerobic compartment (c), it is respectively equipped with sand head aeration head (11) in the 3rd aerobic compartment (d), first
Aerobic compartment (b), the second aerobic compartment (c), dissolved oxygen meter (15) is respectively equipped with the 3rd aerobic compartment (d), first is good
Oxygen compartment (b) and the 3rd aerobic compartment (d) are sequentially connected gas flowmeter (14) and air pump (10) by pipeline respectively；Anoxic every
The return duct switch (7) of room (a) side wall is connected by pipeline through sludge reflux pump (16) with the bottom of second pond (13), and is led to
Sludge from final clarifier mouth (19)；The outer wall of the aerobic compartment (d) is connected with A/O reactors outlet pipe (9), through pipeline and second pond
(13) the middle part connection of side, another upper lateral part of second pond (13) are provided with secondary clarifier effluent pipe (18), sludge from final clarifier mouth
(19) excess sludge of discharge, the penicillin resistant class amonifying bacteria microbial inoculum as produced in batches.
2. the device as claimed in claim 1 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, the second pond
(13) it is that top is two sections of external diameter differences, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth (19) is located at circle
The tapering bottom of cone.
3. the device as claimed in claim 1 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that,
Agitator (12) blade area is 18cm2。
4. the device as claimed in claim 1 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that,
The internal diameter of the sand head aeration head (11) is 4cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720801494.2U CN206956042U (en) | 2017-07-04 | 2017-07-04 | A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720801494.2U CN206956042U (en) | 2017-07-04 | 2017-07-04 | A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum |
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| Publication Number | Publication Date |
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| CN206956042U true CN206956042U (en) | 2018-02-02 |
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| CN201720801494.2U Expired - Fee Related CN206956042U (en) | 2017-07-04 | 2017-07-04 | A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum |
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Granted publication date: 20180202 Termination date: 20190704 |