CN2068238U - Liquid-phase chemical lightening detecting unit - Google Patents
Liquid-phase chemical lightening detecting unit Download PDFInfo
- Publication number
- CN2068238U CN2068238U CN 90201546 CN90201546U CN2068238U CN 2068238 U CN2068238 U CN 2068238U CN 90201546 CN90201546 CN 90201546 CN 90201546 U CN90201546 U CN 90201546U CN 2068238 U CN2068238 U CN 2068238U
- Authority
- CN
- China
- Prior art keywords
- photomultiplier
- helical flow
- liquid
- chemiluminescence
- pond
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses an instrument unit for detecting the chemical lightening of inorganic, organic and physiochemical substances, particularly a chemical lightening detecting unit. The chemical lightening detecting unit is composed of a mixed reaction tube, a helical flow cell, a double-layer light shield, a photomultiplier, a high-voltage power supply and a digital amplifier. By that the design of the mixed reaction tube, the helical flow cell and a light receiving system is optimized, the general type liquid-phase chemical lighting detecting unit is obtained, and the utility model is characterized in simple structure, high sensitivity, good repeatability and convenient operation. The unit is used for detecting the chemical lighting characteristic of some materials. The utility model plays an important role in the trace determination of inorganic substance, metal, medication and the metabolite of the medicine and physiochemical substance.
Description
The utility model is inorganic, the organic and chemiluminescent instrument of biochemical substances about a kind of mensuration.Usually, the advantage of chemiluminescence detector do not need to be light source, and the influence of having eliminated and parasitic light fixed owing to flashing causes unstability of base line fixed, so its remolding sensitivity fluorescence detector height.Some material carries out chemical reaction at normal temperatures, generates reaction intermediate or the reaction product be in excited state, when they when excited state is returned ground state, be attended by the phenomenon of photo emissions.Because the energy of material excited state is by chemical reaction, rather than (for example illumination, the heating etc.) of other approach acquisitions, so be called chemiluminescence.Produce chemiluminescent reagent two classes are arranged: the same hydroperoxidation of oxalate of (1) band aromatic group, the fluorescence excitation material is luminous; (2) some chemical illuminating reagent such as luminol (Luminol), lucigenin and cumarin etc., under hydrogen peroxide and alkali condition, by trace metal ion catalysis, reaction produces chemiluminescence.
All there are various shortcomings in existing phase chromatography-use chemiluminescence detector, causes that to manipulate effect undesirable.For example nearest two pieces of patent documentations (Jap.P. J63-73136, on April 2nd, 1988; J63-73137, on April 2nd, 1988) in, disclosed phase chromatography-use chemiluminescence detector, the structure of its device is made up of parts such as high-voltage power supply, straight tube shape flow cell, condenser, optical filter, photomultiplier and amplifiers.Its major defect is: (1) complex structure, condenser, optical filter are housed in this detector, and reality does not need, and reduces light intensity on the contrary; (2) the flow cell shape is not good, and the mixing efficiency of straight tube shape flow cell is poor, and light-emitting area is little; (3) photomultiplier is far away apart from flow cell, has reduced light intensity greatly.
The purpose of this utility model is to have done important improvement at the shortcoming of above-mentioned prior art, and a kind of novel flow-type liquid chemiluminescence detecting device is provided, and it is than above-mentioned existing chemiluminescence detector is reasonable more on structural design, use is sensitiveer effectively.
The structure of the novel flow-type liquid chemiluminescence detecting device that the utility model provides is shown in figure one and figure two.Figure one is that detector arrangement is always schemed, and 1 is liquid conduits among the figure, and 2 is cast helical flow pond, and 3 is double-deck light shield, and 4 is photomultiplier, and 5 is amplifier, and 6 is reflective mirror.Figure two is that the detailed structure in helical flow pond 2 among total figure is described.The architectural feature of the utility model flow-type liquid chemiluminescence detecting device is: it is made up of cast helical flow pond, optical pickup apparatus and amplifier three parts, cast helical flow pond wherein, shown in figure two, be to constitute an overall flow pond by liquid conduits 1 and cast helical flow pond 2, optical pickup apparatus is to be made of reflective mirror 6, double-deck light shield 3 and photomultiplier 4.Its working method is: fluid sample enters liquid conduits 1, carries out the hybrid reaction outflow by cast helical flow pond 2, and the light that chemical reaction is launched is directly focused on by reflective mirror 6 on the optical window of photomultiplier 4, and signal is via amplifier 5 outputs.Compared with prior art, the further designing technique feature of this liquid chemiluminescence detecting device also is: (1) cast helical flow pond is selected special shape and volume for use: cast helical flow pond is that hard glass, quartz glass or the lucite pipe coiling of 0.5-1mm forms by internal diameter, the volume magnitude range is between the 150-300 microlitre, to guarantee that in principle flow cell is in fixing volume range, should avoid excessive peak expansion, guarantee enough light-emitting areas again; (2) optical pickup apparatus adopts reflective gathering-device (reflective mirror), and double-deck light shield can prevent that any light from leaking, and photomultiplier is near flow cell, and chemiluminescence is directly focused on the receive window of photomultiplier by reflective mirror; (3) the teflon interface is adopted in Zhuan Zhi glass tube and the connection between the metal tube, and sealing is proper, acid and alkali-resistance and organic solvent; (4) instrument adopts high input impedance and low drifting operating amplifier that faint optical signal is become electric signal, thereby measure faint chemiluminescence, make signal attenuation by changing the amplifier feedback resistance, reach 10 to guarantee the solution concentration scope and the detection sensitivity that cover 2-3 the order of magnitude
-9Gram, instrument is furnished with the high voltage adjusting button, is used to regulate the negative high voltage of photomultiplier, and its scope is-500--1000V that signal shows also and directly is connected with integraph with registering instrument through amplification, numeral.Above-mentioned particular design has guaranteed the abundant mixing and the reaction of reactant liquor, and high-level efficiency collects light and no any light leaks, and consequently remolding sensitivity Jap.P. product exceeds 5-10 doubly, for chemiluminescence provides a kind of novel Sensitive Detection apparatus.
Applicating example
1, the utility model has been measured catecholamine in the blood as the detecting device of high performance liquid chromatography, and sensitivity is 0.1pmol.
2, having measured carbazole compound sensitivity as the high performance liquid chromatography detecting device is 1ng.
3, the detecting device of using as Flow Injection Analysis in conjunction with fixing enzyme process, has been measured glucose and cholesterol in the blood.
4, use this detecting device and develop a kind of new chemical luminous system, i.e. umbelliferone--Na
2CO
3Cu
IIMeasure H
2O
2, sensitivity reaches 1 * 10
-8G.
Claims (4)
1, a kind of liquid chemiluminescence detecting device, it is characterized in that it is by cast helical flow pond, optical pickup apparatus and amplifier three parts are formed, wherein cast helical flow pond is to be made of liquid conduits and cast helical flow pond, optical pickup apparatus is to be made of reflective mirror, double-deck light shield and photomultiplier, the photomultiplier position is near flow cell, the teflon interface is adopted in the glass tube of device and metal tube junction, and amplifier is the operational amplifier of high input impedance and low drift.
2, chemiluminescence detector as claimed in claim 1, it is characterized in that cast helical flow pond is the one-piece construction that is made of liquid conduits and helical flow pond, be that hard glass, quartz glass or lucite pipe coiling by internal diameter 0.5-1mm forms, the glass tube flow cell is to adopt multi-circle spiral flow shape structure, the spiral pipe shape can be square and circular, and its volume is between the 150-300 microlitre.
3, liquid chemiluminescence detecting device as claimed in claim 1, it is characterized in that adopting in the optical pickup apparatus structure of double-deck light shield or double-layer seal magazine device, the position of photomultiplier is near the pond of surging, and chemiluminescence is directly focused on the receiver window of photomultiplier by reflective mirror.
4, as claim 1,2,3 described liquid chemiluminescence detecting devices is characterized in that its high input impedance, low drifting operating amplifier can guarantee that the sample solution concentration range and the detection of a topped 2-3 order of magnitude reach 10
-9The sensitivity of gram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90201546 CN2068238U (en) | 1990-02-15 | 1990-02-15 | Liquid-phase chemical lightening detecting unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90201546 CN2068238U (en) | 1990-02-15 | 1990-02-15 | Liquid-phase chemical lightening detecting unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2068238U true CN2068238U (en) | 1990-12-26 |
Family
ID=4883132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90201546 Withdrawn CN2068238U (en) | 1990-02-15 | 1990-02-15 | Liquid-phase chemical lightening detecting unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2068238U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1330963C (en) * | 2001-09-28 | 2007-08-08 | 株式会社日立制作所 | Luminescence detecting device, luminescence detecting mocroarray plate |
| CN106198386A (en) * | 2015-05-07 | 2016-12-07 | 苏州晋翌生物医学仪器有限公司 | Biochemical reaction device and biochemical reaction system |
| CN106645526A (en) * | 2016-11-03 | 2017-05-10 | 平顶山学院 | High performance liquid chromatography countercurrent flow cell |
-
1990
- 1990-02-15 CN CN 90201546 patent/CN2068238U/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1330963C (en) * | 2001-09-28 | 2007-08-08 | 株式会社日立制作所 | Luminescence detecting device, luminescence detecting mocroarray plate |
| CN106198386A (en) * | 2015-05-07 | 2016-12-07 | 苏州晋翌生物医学仪器有限公司 | Biochemical reaction device and biochemical reaction system |
| CN106645526A (en) * | 2016-11-03 | 2017-05-10 | 平顶山学院 | High performance liquid chromatography countercurrent flow cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | A miniaturized liquid core waveguide-capillary electrophoresis system with flow injection sample introduction and fluorometric detection using light-emitting diodes | |
| Dasgupta et al. | Luminescence detection with a liquid core waveguide | |
| US6538735B1 (en) | Method and apparatus for producing and measuring light and for determining the amounts of analytes in microplate wells | |
| US4372745A (en) | Chemical luminescence amplification substrate system for immunochemistry involving microencapsulated fluorescer | |
| CN101271070B (en) | Microcurrent controlled capillary tube electrophoresis liquid core waveguide fluorescence testing apparatus | |
| CN101576557A (en) | Integrated micro-fluidic chip system | |
| WO2011136485A2 (en) | Centrifugal micro-fluidic device and method for immunoassay | |
| WO1999058955A9 (en) | Multi-analyte diagnostic system and computer implemented process for same | |
| CA2748731C (en) | An arrangement and a method for controlling a measurement head of an optical measurement instrument | |
| CA2256629A1 (en) | Masking background fluorescence and luminescence in optical analysis of biomedical assays | |
| US5087425A (en) | Device for flow-injection analysis | |
| US5833924A (en) | Sampling-assay device and interface system | |
| CA1166133A (en) | Chemical luminescence amplification substrate system for immuno chemistry | |
| CN2068238U (en) | Liquid-phase chemical lightening detecting unit | |
| CN106198499A (en) | A kind of micro-fluidic chip for chemiluminescence detection and detection method thereof | |
| CN208636214U (en) | A kind of luminous POCT detection device of homogeneous chemistry | |
| CN105388131A (en) | Fluorescence detection instrument and system based on micro-fluidic chip | |
| CN108212228B (en) | Whole blood separation micro-fluidic chip, detection device and whole blood detection method | |
| US11874216B2 (en) | Automatic reactive oxygen species content detection system suitable for cell microenvironment | |
| Grayeski et al. | Investigation of Instrument Parameters for the Application of Peroxyoxalate Cheminescence Detection to Microbore Chromatography | |
| CN108027375A (en) | Shone measurement using the biological sample of double reagent | |
| JP2001512827A (en) | Detector | |
| Miller | Luminescence measurements on surfaces | |
| Sepaniak | The clinical use of laser-excited fluorometry. | |
| CN103674934B (en) | Micro-fluidic chip Image-forming instrument and system for chemiluminescence detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |