CN206809830U - Separate the moving bed imitation chromatogram separation facility of more mixtures - Google Patents

Abstract

Separate the moving bed imitation chromatogram separation facility of more mixtures, including one group of chromatographic separation device being made up of chromatographic column, boost pump, connecting pipe, connection valve, charging/discharging valve, input and output material pipeline.The import of each chromatographic column is connected with eluant, eluent valve, and it is connected to eluant, eluent house steward；Expect valve, it is connected to material house steward；Circulation fluid valve, it is connected to circulation pipe branch road.Each column outlet is connected with extract solution valve, and it is connected to out extract solution house steward；Raffinate valve, it is connected to raffinate house steward.Go out extract solution house steward and be divided into 2 branch roads：B branch roads, it is connected to B liquid baths；C branch roads, it is connected to C liquid baths.Raffinate house steward is divided into 3 branch roads：Circulation pipe branch road, when circulating valve is opened, feed liquid flows into circulation pipe branch road, and circulation fluid replaces part eluant, eluent；D branch roads, it is connected to D liquid baths；E branch roads, it is connected to E liquid baths.

Description

Separate the moving bed imitation chromatogram separation facility of more mixtures

Technical field

It the utility model is related to technical field of chromatography separation, and in particular to separate the Simulation moving bed color of more mixtures Compose separator.

Background technology

SMBC separation can be divided into continous way and program mode.

Continous way SMBC separate, all logistics include charging, enter eluant, eluent, go out extract solution, go out raffinate, Interior recycle etc., it is continuous.Different according to the purpose of separation, the flow velocity of input and output material and interior recycle can accurate adjustment. The position of input and output material changes over time, continuous repetitive cycling, completes the process of separation.

Continous way SMBC separation since the sixties in last century can commercial Application, be initially to apply in stone Change in industry.

Program mode SMBC separates, and is as the development of computer, automatic control technology gets up.

Program mode SMBC separates, and not all logistics is all continuous.Including three basic segments：Feed zone, Elute section and circulation section.In feed zone, mixture to be separated enters chromatographic column, while raffinate is eluted out.Section is being eluted, Eluant, eluent is added into chromatographic column, and extract is eluted out.In circulation section, material and eluant, eluent do not enter chromatographic column, while There is no product to be extracted, only make interior circulate.

CN203139686U, CN101940850B and CN203220780 are to program mode moving bed imitation chromatogram separation facility It is described.

CN203139686U is only capable of the two components separation for solving fast component and slow component；CN203220780 provides a kind of become The program mode moving bed imitation chromatogram separation facility control method of frequency transformation.It is similar with CN203139686U, CN101940850B As separation method, processing procedure and control technology, it is external since 2005 i.e. be applied to industrial production, country facilities from Start within 2010, be also widely used in glucose/fructose separation, glucose/mannose separation, arabinose/xylose separation etc. In sugar alcohol, food and pharmaceutical products.

CN101940850B except solve two components separation in addition to, additionally provide be easy to scene from four posts, six posts, eight posts it Between switching.But it is this switch in real use and impracticable and have larger defect it is residual in pipeline because after valve transfer The feed liquid stayed can go bad, switch after pipeline and the unnecessary part of valve can increase dead volume, influence separating effect etc..

EP2742982A1 provides a kind of method of efficient SMBC separation, uses 15 ~ 100 μm small The adsorbent of grain is to improve absorption property.After short grained adsorbent, in chromatographic column after pressure drop increase, by shortening color Composing the length of post reduces pressure drop.This method is more practical for the midget plant of use for laboratory, is not suitable for large-scale Industrial production.US7229558B2 provides a kind of program mode chromatography separating method, in several embodiments of this method, has a large amount of Circulation component Recycle produce, embodiment description in circulate component Recycle processing, be use it for dissolving charging Raw material.But in general production, either crystalline mother solution such as molasses, alcohol crystalline mother solution etc., or the chemistry such as acidolysis, enzymolysis or biology The liquid glucose being converted, initial concentration is not high, into chromatographic separation device before also need to concentrate.Circulation component in this way Recycle must collect, handle, concentrate after could be mixed with charge raw material, add process, add energy consumption.

The content of the invention

More component mixing can not be separated well for existing continous way and program mode moving bed imitation chromatogram separation facility The deficiency of thing, the utility model provides the moving bed imitation chromatogram separation facility for separating more mixtures, and is separating During, the direct Returning utilization of eluent that will be circulated in part, reduce the dosage of eluant, eluent.

The moving bed imitation chromatogram separation facility of the more mixtures of separation of the present utility model, by taking 4 chromatographic columns as an example Illustrate, but be not limited to 4 chromatographic columns, can be 6,8,12.

Device forms one group by 4 Coupled columns, there is boost pump, isolating valve, and connecting pipe between each chromatographic column. In addition with feed system, enter the conventional auxiliary equipment such as eluent system, discharging storage tank, pressure measxurement, flow measurement.

The length of each chromatographic column is identical with diameter.

Each chromatographic column import is connected with eluant, eluent valve VW1 ~ VW4, and eluant, eluent valve is connected to eluant, eluent house steward, when unlatching is washed During de- agent valve, chromatographic column input eluant, eluent.

Each chromatographic column import is connected with material valve VF1 ~ VF4, and material valve is connected to material house steward, when valve is expected in unlatching, chromatographic column Input material liquid.

Eluant, eluent house steward and material house steward are controlled by entering eluent system and feed system, are always maintained at eluant, eluent house steward and material House steward has certain pressure and flow, can input eluant, eluent or raw material into chromatographic column at any time.

Each chromatographic column import is also associated with circulation fluid valve VR1 ~ VR4, and circulation fluid valve is connected to circulation pipe branch road, works as circulation When liquid main valve VR is opened, feed liquid flows into circulation pipe branch road, through one in circulation fluid valve VR1 ~ VR4, flows into chromatographic column, this circulation Liquid replaces part eluant, eluent.

Each column outlet is connected with extract solution valve VM1 ~ VM4, and extract solution valve is connected to out extract solution house steward, extract solution Equipped with flow instrument, flow control valve on house steward, for detecting, adjusting flow；Extract solution house steward is divided into 2 branch roads：B branch roads pass through It is connected after VB valves with B liquid baths, C branch roads are connected after VC valves with C liquid baths.

Each column outlet is connected with raffinate valve VN1 ~ VN4, and raffinate valve is connected to raffinate house steward；Raffinate is total Equipped with flow instrument, flow control valve on pipe, for detecting, adjusting flow；Raffinate house steward is divided into 3 branch roads：Circulation fluid is total Valve VR forms a branch road with circulation pipe branch road, and circulation pipe branch road connects with the circulation fluid valve VRn of each chromatographic column；D branch roads pass through It is connected after VD valves with D liquid baths；E branch roads are connected after VE valves with E liquid baths.

In order to apparent, following title is explained at this：

Circulation fluid：It is that feed zone flows out, return for the feed liquid instead of part eluant, eluent；

Interior circulation fluid：The feed liquid of interior circulation when device does not feed, do not enter eluant, eluent, do not discharge；

B liquid：Elution section flows out, through going out extract solution house steward, VB valves, into the feed liquid of B liquid baths；

C liquid：Elution section flows out, through going out extract solution house steward, VC valves, into the feed liquid of C liquid baths；

D liquid：What feed zone flowed out, through raffinate house steward, VD valves, into the feed liquid of D liquid baths；

E liquid：What feed zone flowed out, through raffinate house steward, VE valves, into the feed liquid of E liquid baths；

Large period：Plant running step 1 ~ step 6 is a cycle, runs four cycles altogether, is a large period.

When B liquid valves VB is opened, feed liquid flows into B liquid baths through B branch roads.

When C liquid valves VC is opened, feed liquid flows into C liquid baths through C branch roads.

When D liquid valves VD is opened, feed liquid flows into D liquid baths through D branch roads.

When E liquid valves VE is opened, feed liquid flows into E liquid baths through E branch roads.

It is big for detecting, adjusting the flow of extract solution of outflow equipped with flow instrument, flow control valve on extract solution house steward It is small；Equipped with flow instrument, flow control valve, the uninterrupted of the raffinate for detecting, adjusting outflow on raffinate house steward.

In order to reduce eluting agent, circulation fluid is used directly as eluant, eluent.Each chromatographic column import, which removes, to be connected with Outside eluant, eluent valve VW1 ~ VW4 and material valve VF1 ~ VF4, circulation fluid valve VR1 ~ VR4 is also connected with, when circulation fluid main valve VR is opened, is opened Open the circulation fluid valve of some chromatographic column import, you can elute extract solution with circulation fluid.By this part circulation fluid Returning utilization, take For part eluant, eluent, the dosage of eluant, eluent can be reduced.The amount of the circulation fluid of this part is accurate measurement, so that its composition is with washing De- agent approaches, without bringing raffinate followed by into elution section.

Device of the present utility model is controlled by computer, automatic running.Plant running comprises at least three processes：Charging, Elution, interior circulation.Complete these three processes and be referred to as a cycle.Three processes of a cycle are made up of 6 steps again.

4 chromatographic columns are divided into Z1 areas, Z2 areas, Z3 areas, Z4 areas.

Z1 areas are elution section, and eluant, eluent enters in chromatographic column from Z1 areas top, goes out extract solution from Z1 areas bottom.Extract solution according to Secondary is B liquid and C liquid.B liquid and C liquid flow into B liquid baths and C liquid baths through respective branch path B branch roads and C branch roads.

Z2 areas are mixing section.

Z3 areas are feed zone, and mixture to be separated enters in chromatographic column from this section of top, goes out circulation fluid from Z4 areas bottom.Follow Ring liquid returns to Z1 areas and is used as eluant, eluent.

Z4 areas are compartmented.

After flowing out a certain amount of circulation fluid from Z4 areas bottom, switch to go out raffinate from Z3 areas bottom, be followed successively by D liquid and E liquid. D liquid and E liquid flow into D liquid baths and E liquid baths through respective branch path road D branch roads and E branch roads.

Now, feed zone and elution section are to disconnect, and charging and elution are carried out simultaneously.

After 3 ~ 5 large periods of plant running, that is, reach poised state.Under poised state, each cycle reruns, each The B liquid of outflow, C liquid, D liquid, E liquid, the volume of circulation fluid, purity, mass percent concentration are stable in chromatographic column.It is any In period, input equal with the volume of material amount of outflow chromatographic column.

Isolating valve, between chromatographic column is opened, and Z1 ~ Z4 areas are together in series, and forms interior circulate.Feed and enter during interior circulation Eluant, eluent stops, and is also flowed out without material out of post, and system makees interior circulate.During interior circulation, these four materials of B, C, D, E due to They have different migration velocities in polymeric adsorbent, the Adsorption and desorption effect through polymeric adsorbent in chromatographic column, gradually quilt Separate.

Eluant, eluent includes：Water, the aqueous solution of methanol, the aqueous solution of ethanol, diluted acid etc..

Eluant, eluent should be able to dissolve with incoming mixture, and be easy to subsequent treatment.Because most sugars are soluble in water, and water It is relatively convenient in post processing, so water is the eluant, eluent commonly used in carbohydrate separation.

Adsorbing medium is selected according to the property of mixture to be separated.Adsorbing medium can be Cation adsorption resin or Anion-adsorption resin or cationic molecule sieve.Polymeric adsorbent may be selected weakly acidic, can also select highly acid.

Separation temperature is selected according to the absorption feature of the property of mixture to be separated and adsorbent to mixture.As mixed Compound is heat-sensitive substance, then separation temperature is preferably low, and separation temperature on the contrary may be selected higher.But separation temperature is too high, to inhaling The life-span of attached resin can have an impact.Separation temperature is advisable at 20 ~ 90 DEG C.

Pressure in system is as caused by boost pump, charging, intake pump.Pressure can be 0.1 ~ 1MPa in system.

Linear velocity of the material in system is influential on adsorbing and freeing.The linear velocity of material is 1 ~ 5m/ in system h。

The utility model can be used for following material but be not limited only to the separation of following material：The sulfurous method boiling of papermaking Liquid, the various molasses containing monosaccharide and disaccharide and polysaccharide, fructose syrup, gluco-manno sugar polysaccharide liquid, inverted sugar mixture, maltose Slurry, maltitol liquor, mannitol sorbierite mixed liquor, production of sugar polyol crystalline mother solution, organic acid, plant extraction liquid etc..

Incoming mixture in the utility model is not limited to include four kinds of materials, can be four or more material composition Mixture.Tetra- kinds of materials containing B, C, D, E in the charging mixed liquor of the utility model description.Adsorbent used is to these four things The absorption affinity of matter is followed successively by by strong to weak：C、B、 E、D.

Volume, the volume of C liquid, the volume of D liquid, the volume of E liquid of separation product B liquid, the volume of circulation fluid Amount, be measured via flow instrument, accumulate after gained, and opened with the control of its volume corresponding VMn, VNn, VC, VD, VE, VR Open and close.Meanwhile these volumes are also the foundation of the corresponding inlet amount of control, inflow or circulation fluid back amount.System The amount of interior circulation be also measured via flow instrument, accumulate after gained, this amount be used for control system interior circulation volume.Enter The amount for entering some chromatographic column is identical with the amount for going out the chromatographic column, therefore only detects and control load.

Feed liquid containing a variety of mixtures, it can be separated well in a device, crux is：Suitable column length Rational material distribution, suitable adsorbent, suitable absorbent particles size, feed liquid suitable linear speed in post in degree, post Degree etc..Accurate metering and control also play a very important role.

The utility model provides a kind of moving bed imitation chromatogram separation facility for separating more mixtures, contains in mixture There is the material of 4 kinds or more than 4 kinds, select suitable adsorbent and separation condition, by different material separating-purifyings.This reality simultaneously Part eluant, eluent is replaced using circulation fluid with new, the dosage of eluant, eluent can be saved.

Brief description of the drawings

Fig. 1 is the moving bed imitation chromatogram separation facility installation diagram for separating more mixtures.

One ~ step 3 of the step of Fig. 2 is cycle one.

Black matrix shows have material to flow through in chromatographic column.

Four ~ step 6 of the step of Fig. 3 is cycle one.Black matrix shows have material to flow through in chromatographic column.

Each part is as follows in accompanying drawing：Chromatographic column 1, boost pump 2, connecting tube 3, flow instrumentation 4, flow control valve 5, every Disconnected valve 6, eluant, eluent house steward 11, expects house steward 12, expects valve VF1 ~ VF4, eluant, eluent valve VW1 ~ VW4, circulation fluid valve VR1 ~ VR4, extraction Liquid valve VM1 ~ VM4, raffinate valve VN1 ~ VN4, go out extract solution house steward 31, raffinate house steward 32, circulation fluid main valve VR, circulation pipe branch Road 311；C liquid valve VC, C branch road 312；D liquid valve VD, D branch road 313；E liquid valve VE, E branch road 314；B branch roads 315；B liquid baths, C liquid Groove, D liquid baths, E liquid baths.

Embodiment

Embodiment one

Device of the present utility model is described in further detail below in conjunction with the accompanying drawings.

Fig. 2 is six steps of a cycle, referred to as cycle one.

In each step in cycle one, No. 1 chromatographic column is Z1 areas, and No. 2 chromatographic columns are Z2 areas, and No. 3 chromatographic columns are Z3 areas, No. 4 Chromatographic column is Z4 areas.

Step 1：Z1 enters eluant, eluent（Open VW1 valves）, Z1 dischargings（Open VM1 valves, VB valves）, B liquid is total through going out extract solution Pipe 31, B branch roads 315 are discharged into B liquid baths；Z3 is fed（Open VF3 valves）, Z4 dischargings（Open VN4 valves, VE valves）By upper a cycle pipe The a small amount of feed liquid retained in road is discharged into E liquid baths.Time used in this process is very short, will can be stayed in a cycle pipeline within 1 ~ 3 second The a small amount of feed liquid deposited is drained, then operating procedure 2.

Step 2：Z3 is fed（Open VF3 valves）, Z4 dischargings（Open VN4 valves, VR valves）By circulation fluid through circulation pipe branch road 311st, VR1, Z1 is inputted；Z1 discharges（Open VM1 valves, VB valves）, by B liquid through going out extract solution house steward 31, B branch roads 315 are discharged into B liquid Groove.

Step 3：Z3 is fed（Open VF3 valves）, Z3 dischargings（Open VN3 valves, VD valves）By D liquid through raffinate house steward 32, VD Valve, D branch roads 312, are discharged into D liquid baths；Z1 is intake（Open VW1 valves）, Z1 dischargings（Open VM1 valves, VB valves）, by B liquid through going out extraction Liquid house steward 31, VB valves, B branch roads 315 are discharged into B liquid baths.

Step 4：Z3 is fed（Open VF3 valves）, Z3 dischargings（Open VN3 valves, VD valves）By D liquid through raffinate liquid material house steward 31, VD valves, D branch roads 313, it is discharged into D liquid baths；Z1 enters eluant, eluent（Open VW1 valves）, Z1 dischargings（Open VM1 valves, VC valves）, C liquid is passed through Go out extract solution house steward 31, VC valves, C branch roads 312 are discharged into C liquid baths.

When operating procedure 3 and step 4, in accordance with the principle for arriving first first grade.I.e. step 3 when, if B liquid measures first reach setting Value, then Z1 areas stop waiting, until D liquid measures reach setting value, step 4 of reruning；If D liquid measures first reach setting value, Z3 Area stops waiting, until after B liquid measures reach setting value, step 4 of reruning.

Another situation is, before D liquid measures reach setting value, inlet amount F has reached setting value, and now Z3 stops waiting, directly After into step 4, C liquid measures reach setting value, operating procedure 4-1.

Step 4-1：Z1 enters eluant, eluent（Open VM1 valves, VC valves）, by C liquid through going out extract solution house steward 31, VC valves, C branch roads 312 are discharged into C liquid baths.

Step 5：Z1 enters eluant, eluent（Open VW1 valves）, Z3 dischargings（Open VN3 valves, VE valves）, by E liquid through raffinate house steward 32nd, VE valves, E branch roads 314 are discharged into E liquid baths.

Step 6：Material does not pass in and out system, carries out interior circulate.

After the completion of cycle one, the cycle of operation two.Cycle two is that tetra- areas of Z1, Z2, Z3, Z4 advance to next chromatographic column One step, the running of step 1 ~ step 6 is identical with the cycle one, but all toward going a step further before previous chromatographic column.Run successively Cycle three and cycle four.

After the completion of cycle four is run, return period one, circulation is carried out.One ~ cycle of cycle four forms a large period.Operation After 3 ~ 5 large periods, system reaches poised state.

After SMBC separates, 4 kinds of separation products are obtained：B liquid, C liquid, D liquid and E liquid.System reaches balance Afterwards, this 4 kinds of components have metastable volume, weight percent concentration, a purity, can detect according to a conventional method out. By B liquid, C liquid, the amount of D liquid and E liquid, the opening and closing of VB, VC, VD, VE valve are controlled, B liquid, C liquid, D liquid and E liquid can flow Enter corresponding basin.

As further improvement of the utility model, raffinate can further be segmented.Now, extract solution is：B liquid, C Liquid, raffinate are D liquid, E liquid, F liquid.

Embodiment two

Following examples are with the moving bed imitation chromatogram separation facility of a pilot-scale come to device of the present utility model It is further described.

Device is made up of 4 chromatographic columns, is connected between each chromatographic column by boost pump with pipeline, each chromatographic column connection There is input and output material valve；Also feed system, water inlet system, flow pressure detect regulating system and discharge system simultaneously.Device Installation diagram is as shown in Figure 1.

Each chromatogram column length is 3m, internal diameter 0.072m.Polymeric adsorbent is filled in each chromatographic column.Resin is highly acid Cationic ion-exchange resin, sodium form, the mol/ml of volume full exchange capacity >=1.8.Resin is equal grain spheroid, diameter 0.32mm.From Pure water is as eluant, eluent.

Raw material is molasses, the accessory substance from soybean processing, contains oligosaccharide（Gossypose+stachyose）, glucose, fruit More components such as sugar, sucrose.The mass percent concentration of raw material is 60.1%.Separation temperature is at 60 ~ 65 DEG C.Molasses raw material composition is such as Shown in table 1.

The molasses raw material component list of table 1

Cycle one runs following steps：

Step 1：0.02L molasses raw materials input Z3, and 0.02L out-feed liquids are discharged into E liquid baths；0.02L water inputs Z1,0.02L's B liquid is discharged into B liquid baths.

Step 2：0.3L molasses raw materials input Z3,0.3L circulation fluid input Z1；0.3L B liquid is discharged into B liquid baths.

Step 3:0.48L molasses raw materials input Z3, and 0.48L feed liquid is discharged into D liquid baths；0.6L water inputs Z1,0.6L B Liquid is discharged into B liquid baths.

Step 4：0.4L molasses raw materials input Z3, and 0.48L feed liquid is discharged into D liquid baths；0.58L water inputs Z1,0.58L material Liquid is discharged into C liquid baths.

Step 5：1.4L water inputs Z1, and 1.4L feed liquid is discharged into E liquid baths.

In step 6, material does not pass in and out system, carries out interior circulate.The amount of interior circulation is 3.6L.

Respective 6 steps in two ~ cycle of cycle four are identical with the cycle 1.After the completion of cycle four, the repetition period one is gone back to.

One ~ cycle of cycle four forms a large period.

After running 3 ~ 5 large periods, system reaches poised state.After system balancing, separating obtained each component is taken, with height Effect liquid phase chromatogram is discussed（HPLC）Detection, oligosaccharide（Gossypose+stachyose）Component, glucose component, fructose component, sucrose component Concentration, purity and yield as shown in table 2, table 3.

It is for the separation product B liquid described, C liquid, D liquid and E liquid, its corresponding relation：D liquid oligosaccharide（Gossypose+water Threose）, B liquid glucose, C juicy fruits sugar, E liquid sucrose.

The concentration and yield of the separation product of table 2

The purity of the separation product of table 3

After separation, oligosaccharide in oligosaccharide component（Gossypose+stachyose）Purity 90.16%DS, product are available for diabetes People eats；Glucose purity 82.6%DS in glucose component, available for production crystal glucose product；Fructose in fructose component It purity 88.1%DS, can be compounded with F42 fructose syrups, produce F55 fructose syrup products；Sucrose purity 79.77%DS in sucrose component, Available for production candy product.

Embodiment of the present utility model is not to limitation of the present utility model.Those skilled in the art can be by this reality Implement changing form for various ways with new method.

Claims (5)

1. the moving bed imitation chromatogram separation facility of the more mixtures of separation, one group is formed by the connection of single chromatographic column, often There are boost pump, isolating valve, and connecting pipe between individual chromatographic column, each chromatographic column is respectively connected with material valve, eluant, eluent valve, circulation fluid Valve, extract solution valve, raffinate valve, also discharge basin, it is characterised in that：

(1) described device forms one group by multiple chromatographic columns, and each chromatographic column import is connected with eluant, eluent valve VW1 ~ VWn, material valve VF1 ~ VFn, circulation fluid valve VR1 ~ VRn；The eluant, eluent valve and material valve connect with eluant, eluent house steward (11) and material house steward (12) respectively Connect；The circulation fluid valve VR1 ~ VRn is connected with circulation pipe branch road (311)；N=chromatographic column quantity；

(2) each column outlet of described device is connected with extract solution valve VM1 ~ VMn and raffinate valve VN1 ~ VNn；The extraction Liquid valve is connected with going out extract solution house steward (31)；The raffinate valve VN1 ~ VNn is connected with raffinate house steward (32)；

(3) go out described in equipped with flow instrumentation F2, flow control valve on extract solution house steward (31), for detecting, adjusting flow； Extract solution house steward (31) is divided into 2 branch roads：B branch roads (315) are connected after VB valves with B liquid baths, C branch roads (312) after VC valves with C Liquid bath connects；

(4) equipped with flow instrumentation F1, flow control valve on the raffinate house steward (32), for detecting, adjusting flow；Carry Extraction raffinate house steward (32) is divided into 3 branch roads：Circulation fluid main valve VR forms a branch road, the circulation pipe with circulation pipe branch road (311) Branch road (311) connects with the circulation fluid valve VRn of each chromatographic column；D branch roads (313) are connected after VD valves with D liquid baths；E branch roads (314) it is connected after VE valves with E liquid baths；

（5）Plant running process comprises at least three steps：Charging, elution, interior circulation；

（6）Circulation fluid returns after circulation fluid main valve VR from circulation pipe branch road (311), for directly substituting part eluant, eluent.

2. the moving bed imitation chromatogram separation facility of the more mixtures of separation according to claim 1, it is characterised in that Described device is made up of 4 ~ 12 Coupled columns；Wherein there is a circular flow instrumentation between any two chromatographic columns FR。

3. the moving bed imitation chromatogram separation facility of the more mixtures of separation according to claim 1, it is characterised in that The volume of extract solution house steward (31) outflow measure via flow instrumentation F2 and obtained by after accumulating；Raffinate house steward (32) volume of outflow measure via flow instrumentation F1 and obtained by after accumulating；The volume of interior circulation fluid is via following Gained after circulation instrumentation FR is measured and accumulated；The cumulant of flow, for control corresponding valve opening and close from And the beginning and stopping circulated in the disengaging of control material, or control.

4. the moving bed imitation chromatogram separation facility of the more mixtures of separation according to claim 1, it is characterised in that Fill adsorbing medium in the chromatographic column, the polymeric adsorbent is highly acidic cation polymeric adsorbent, or weakly-basic anion is inhaled Attached resin, or acrylic acid Anion-adsorption resin, or cationic molecule sieve.

5. the moving bed imitation chromatogram separation facility of the more mixtures of separation according to claim 1, it is characterised in that The eluant, eluent is pure water or methanol aqueous solution or ethanol water or organic acid soln or inorganic acid solution.