CN206778636U - A kind of liquid nitrogen grinding device for being used to extract RNA - Google Patents
A kind of liquid nitrogen grinding device for being used to extract RNA Download PDFInfo
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- CN206778636U CN206778636U CN201720561113.8U CN201720561113U CN206778636U CN 206778636 U CN206778636 U CN 206778636U CN 201720561113 U CN201720561113 U CN 201720561113U CN 206778636 U CN206778636 U CN 206778636U
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- liquid nitrogen
- mortar
- refrigerating plant
- grinding device
- extract rna
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Abstract
A kind of liquid nitrogen grinding device for being used to extract RNA is the utility model is related to, belongs to use for laboratory lapping device technical field of structures.Including the mortar being made of ceramic materials, refrigerating plant, for conveying the liquid nitrogen conveyer of liquid nitrogen into mortar and refrigerating plant, wherein, mortar mutually spins with refrigerating plant and mortar is located in refrigerating plant, the top of mortar is higher than the upper surface of refrigerating plant, liquid nitrogen conveyer includes being used for the splendid attire vessel for containing liquid nitrogen, the bottom for containing vessel is provided with liquid nitrogen output pipe and the valve for controlling liquid nitrogen output pipe break-make, the switching knob for being passed through liquid nitrogen is offered on refrigerating plant, the stopping means for fixing liquid nitrogen output pipe is equipped with refrigerating plant and mortar.The utility model easily adds liquid nitrogen, prevents from causing sample overflow, prevent liquid nitrogen from quickly volatilizing when adding liquid nitrogen, reduce addition liquid nitrogen number, prevent sample from spilling, accelerate grinding rate, facilitate operator to use, and improves the protective effect to operator.
Description
Technical field
A kind of liquid nitrogen grinding device for being used to extract RNA is the utility model is related to, belongs to use for laboratory lapping device structure
Technical field.
Background technology
In molecular biology, RNA extractive techniques are a very important basic operation technologies, many experiments need from
RNA is extracted in sample tissue, RNA quality is concerned in follow-up reverse transcription, PCR, gene expression detection, MicroRNA high fluxs
The success or failure of the experiments such as sequencing and accuracy.Because RNA is most susceptible to the degraded of RNase, and RNase has very strong activity,
And it is ubiquitous, therefore in order to suppress RNase activity to greatest extent, sample tissue must be ground brokenly in liquid nitrogen
It is broken.
Laboratory is using the method for more liquid nitrogen grinding tissue：Liquid nitrogen is poured into the mortar for having removed RNase,
Precooling is carried out to mortar, then the tissue of -80 DEG C of preservations or flesh tissue are added in mortar, using having removed RNase
Pestle carry out smashing grinding to pieces, be continuously added in the process liquid nitrogen keep tissue sample be constantly in liquid nitrogen.Traditional grinds
Alms bowl is that internal diameter is 6 centimetres（It is equipped with a diameter of 2cm pestle head）Or 8 centimetres（It is equipped with a diameter of 3cm pestle head）, depth
For 3-4 centimetres of bowl structure, liquid nitrogen can vapor away quickly after adding, and when smashing tissue to pieces with liquid nitrogen early stage, smash to pieces
Cheng Zhong, the small tissue blocks smashed to pieces are easy to spill, and liquid nitrogen is also easily spilt during smashing to pieces, so not only causes sample
Representative decline, and easily make operator's frostbite.Because liquid nitrogen vapors away quickly, liquid is ceaselessly added in tissue grinder
Nitrogen, operator can be made in a rush, extend RNA extraction time, add the risk of RNA degradeds.Adding liquid nitrogen simultaneously
During, when tissue grinder it is thinner when, it is easy to overflowed together with liquid nitrogen, cause sample waste and also reduce sample
Representativeness.
It would therefore be highly desirable to design a kind of easily addition liquid nitrogen, prevent from causing sample to overflow, prevent that liquid nitrogen is quick when adding liquid nitrogen
Volatilization, addition liquid nitrogen number is reduced, prevents sample from spilling, accelerates grinding rate, facilitates operator to use, improved to operator's
The liquid nitrogen grinding device of protective effect.
The content of the invention
The purpose of this utility model is solve above-mentioned the shortcomings of the prior art part, there is provided a kind of easy annex solution
Nitrogen, prevent from causing sample overflow, prevent liquid nitrogen from quickly volatilizing when adding liquid nitrogen, reduces add liquid nitrogen number, prevent sample from spilling,
Accelerate grinding rate, facilitate operator to use, improve the liquid nitrogen grinding dress for being used to extract RNA of the protective effect to operator
Put.
The utility model is achieved through the following technical solutions：
A kind of liquid nitrogen grinding device for being used to extract RNA, it is characterized in that the mortar including being made of ceramic materials
1st, refrigerating plant, the liquid nitrogen conveyer for conveying liquid nitrogen into mortar 1 and refrigerating plant made of stainless steel material, its
In, mortar 1 mutually spins with refrigerating plant and mortar 1 is located in refrigerating plant, and the top of mortar 1 is higher than the upper table of refrigerating plant
Face, liquid nitrogen conveyer include being used to contain the splendid attire vessel 2 of liquid nitrogen, contain vessel 2 bottom be provided with liquid nitrogen output pipe 3 and
For controlling the valve 4 of the break-make of liquid nitrogen output pipe 3, the switching knob for being passed through liquid nitrogen is offered on refrigerating plant, is freezed
The stopping means for fixing liquid nitrogen output pipe 3 is equipped with device and mortar 1；
As a kind of preferable technical scheme of the utility model, the top of the mortar 1 is provided with lid 5, is opened up on lid 5
Have for the through hole 6 through grinding rod；
As a kind of preferable technical scheme of the utility model, the refrigerating plant include be made up of stainless steel material and
Inside is loaded with the refrigeration housing 7 of liquid nitrogen, and refrigeration housing 7 is provided with heat preservation sandwich layer 8, and the top of refrigeration housing 7, which offers, to be used for
Through the circular mounting hole 9 of mortar 1, internal thread is laid with the inwall of mounting hole 9；
As a kind of preferable technical scheme of the utility model, the mortar 1 includes hollow cylindrical bowl and located at alms bowl
Bowl-shape alms bowl bottom below body, bowl are structure as a whole with alms bowl bottom, and the outer wall of bowl is provided with to be made with the screw-internal thread fit of mounting hole 9
External screw thread；
As a kind of preferable technical scheme of the utility model, the switching knob includes being opened in the refrigeration upper table of housing 7
Trepanning 10 on face and the knob 11 to be spun with the phase of trepanning 10, the inwall of trepanning 10 are provided with internal thread, the outer wall of knob 11
It is provided with the external screw thread used with the screw-internal thread fit of trepanning 10；
As a kind of preferable technical scheme of the utility model, the stopping means includes n-type ring I 12, n-type ring II 13,
Wherein n-type ring I 12 is installed vertically on opening position of the upper surface of lid 5 close to through hole 6, and n-type ring II 13 is installed vertically on close open
Close the opening position of knob；
As a kind of preferable technical scheme of the utility model, the lower surface of the refrigerating plant is provided with improved mat 14；
As a kind of preferable technical scheme of the utility model, the splendid attire vessel 2 are included by double-layer stainless steel material system
Into kettle shell, heat-insulation layer is provided with kettle shell, the back side of kettle shell is provided with for splendid attire vessel to be hung on into wall
Suspension member 15 on face or support frame；
As a kind of preferable technical scheme of the utility model, the side for containing vessel 2, which is provided with, to be used to hang liquid nitrogen
The support 16 of output pipe 3；
As a kind of preferable technical scheme of the utility model, the height of the mortar 1 is 8-10 centimetres, and mortar 1 is higher than
2-4 centimetres of refrigerating plant upper surface.
A kind of liquid nitrogen grinding apparatus structure for extracting RNA of the present utility model is ingenious in design, has below beneficial to effect
Fruit：
1st, liquid nitrogen conveyer makes liquid nitrogen be easier to add, and liquid is directly added into mortar relative to liquid nitrogen vacuum flask
Nitrogen, the phenomenon of liquid nitrogen spilling and liquid nitrogen together with one piece of spilling of sample will not be produced；
2nd, by the way that mortar is placed in refrigerating plant, and liquid nitrogen is put into refrigerating plant, liquid nitrogen volatilization can be substantially reduced
Speed, reduce liquid nitrogen and add number, shorten milling time, while improving operating efficiency, substantially reduce the wind that RNA is degraded
Danger；
3rd, mortar is increased, and is capped, and is avoided during smashing to pieces and tissue occurs and collapse the phenomenon, while prevents liquid nitrogen from adding
During entering, the phenomenon of liquid nitrogen and sample spilling；
4th, each part is used cooperatively, it is easier to is operated, is advantageous to protect operator, and shorten sample processing time, reduces
The risk of RNA degradeds.
In summary, the utility model smart structural design, in laboratory, field of milling has good application prospect.
Brief description of the drawings
Fig. 1：The utility model is used for the structural representation for extracting RNA liquid nitrogen grinding device.
In figure：1st, mortar, 2, contain vessel, 3, liquid nitrogen output pipe, 4, valve, 5, lid, 6, through hole, 7, system
Cooled case, 8, heat preservation sandwich layer, 9, mounting hole, 10, trepanning, 11, knob, 12, n-type ring I, 13, n-type ring II, 14, slipmat
Piece, 15, suspension member, 16, support.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are obtained under the premise of no creative work
The every other embodiment obtained, belong to the scope of the utility model protection.
Embodiment 1：Refer to the attached drawing 1.The liquid nitrogen grinding device for being used to extract RNA of the present embodiment, including following structure：Grind
Alms bowl 1, vessel 2 are contained, liquid nitrogen output pipe 3, valve 4, lid 5, through hole 6, freeze housing 7, heat preservation sandwich layer 8, mounting hole 9, rotation
Hole 10, knob 11, n-type ring I 12, n-type ring II 13, improved mat 14, suspension member 15, support 16.
Installation and annexation：The liquid nitrogen grinding device for being used to extract RNA of the present embodiment, mortar 1 and refrigerating plant phase
Spin and mortar 1 is located in refrigerating plant, the top of mortar 1 is higher than the upper surface of refrigerating plant, and the height of mortar 1 is 8-10 lis
Rice, mortar 1 are higher than 2-4 centimetres of refrigerating plant upper surface, and liquid nitrogen conveyer includes being used for the splendid attire vessel 2 for containing liquid nitrogen, contained
The bottom of dress vessel 2 is provided with liquid nitrogen output pipe 3 and the valve 4 for controlling the break-make of liquid nitrogen output pipe 3, is opened on refrigerating plant
Provided with the switching knob for being passed through liquid nitrogen, it is equipped with refrigerating plant and mortar 1 for fixing the spacing of liquid nitrogen output pipe 3
Device；The top of mortar 1 is provided with lid 5, is offered on lid 5 for the through hole 6 through grinding rod；Refrigerating plant is included by not
Rust Steel material is made and the internal refrigeration housing 7 for being loaded with liquid nitrogen, and refrigeration housing 7 is provided with heat preservation sandwich layer 8, refrigeration housing 7
Top is offered for the circular mounting hole 9 through mortar 1, and internal thread is laid with the inwall of mounting hole 9；During mortar 1 includes
Hollow-cylindrical bowl and the bowl-shape alms bowl bottom below bowl, bowl are structure as a whole with alms bowl bottom, the outer wall of bowl be provided with
The external screw thread that the screw-internal thread fit of mounting hole 9 uses；Switching knob include the trepanning 10 that is opened on refrigeration housing 7 upper surface with
And the knob 11 to be spun with the phase of trepanning 10, the inwall of trepanning 10 are provided with internal thread, the outer wall of knob 11 is provided with and trepanning 10
The external screw thread that uses of screw-internal thread fit；Stopping means includes n-type ring I 12, wherein n-type ring II 13, the right angle setting of n-type ring I 12
Opening position in the upper surface of lid 5 close to through hole 6, n-type ring II 13 are installed vertically on the opening position close to switching knob；Refrigeration dress
The lower surface put is provided with improved mat 14；Containing vessel 2 includes kettle shell, kettle shell made of double-layer stainless steel material
Interior to be provided with heat-insulation layer, the back side of kettle shell is provided with the suspension member for splendid attire vessel to be hung on metope or support frame
15；The side for containing vessel 2 is provided with the support 16 for being used for hanging liquid nitrogen output pipe 3.
Application method：
1st, add liquid nitrogen in vessel are contained, according to processing sample number, determine add liquid nitrogen volume, in case subsequently
Use；
2nd, refrigerating plant is placed on；
3rd, the mortar for having removed RNase is tightened in refrigerating plant；
4th, the liquid nitrogen that mortar volume half is added in mortar carries out precooling, during liquid feeding nitrogen, by the outlet of liquid nitrogen output pipe 3
Inserted in after through n-type ring I 12 in through hole 6, open valve 4, liquid nitrogen is passed through into mortar；
5th, add liquid nitrogen in refrigerating plant, during liquid feeding nitrogen, open knob 11, by the outlet of liquid nitrogen output pipe 3 through n
Inserted in after type ring II 13 in trepanning 10, open valve 4, liquid nitrogen is passed through into refrigerating plant；
6th, transfer the sample into mortar；
7th, lid is covered to smash to pieces；
8th, it is ground；
9th, take appropriate ground sample to be transferred in the centrifuge tube of no RNase, liquid nitrogen flash freezer, be transferred to -80 DEG C of refrigerators
It is standby；
10th, liquid nitrogen Frostbite preventing gloves are worn, former mortar is taken out；
11st, the mortar more renewed, the next sample of start to process.
Preferred embodiment of the present utility model is the foregoing is only, it is all in this practicality not to limit the utility model
Within new spirit and principle, any modification, equivalent substitution and improvements made etc., guarantor of the present utility model should be included in
Within the scope of shield.
Claims (10)
1. a kind of liquid nitrogen grinding device for being used to extract RNA, it is characterised in that including the mortar being made of ceramic materials（1）, by
Refrigerating plant made of stainless steel material, for mortar（1）And the liquid nitrogen conveyer of liquid nitrogen is conveyed in refrigerating plant, its
In, mortar（1）Mutually spun with refrigerating plant and mortar（1）In refrigerating plant, mortar（1）Top higher than refrigerating plant
Upper surface, liquid nitrogen conveyer include being used for the splendid attire vessel for containing liquid nitrogen（2）, contain vessel（2）Bottom be provided with liquid nitrogen it is defeated
Go out pipeline（3）And for controlling liquid nitrogen output pipe（3）The valve of break-make（4）, offer on refrigerating plant for being passed through liquid nitrogen
Switching knob, refrigerating plant and mortar（1）On be equipped with for fixing liquid nitrogen output pipe（3）Stopping means.
2. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the mortar（1）
Top be provided with lid（5）, lid（5）On offer for the through hole through grinding rod（6）.
3. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the refrigerating plant
Including being made up of stainless steel material and the internal refrigeration housing for being loaded with liquid nitrogen（7）, freeze housing（7）It is provided with heat preservation sandwich layer
（8）, freeze housing（7）Top offer for through mortar（1）Circular mounting hole（9）, mounting hole（9）Inwall on cloth
Provided with internal thread.
4. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 3, it is characterised in that the mortar 1 wraps
Include hollow cylindrical bowl and the bowl-shape alms bowl bottom below bowl, bowl are structure as a whole with alms bowl bottom, set on the outer wall of bowl
Have and mounting hole（9）The external screw thread that uses of screw-internal thread fit.
5. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the switching knob
Including being opened in refrigeration housing（7）Trepanning on upper surface（10）And and trepanning（10）The knob mutually to spin（11）, trepanning
（10）Inwall be provided with internal thread, knob（11）Outer wall be provided with and trepanning（10）The outer spiral shell that uses of screw-internal thread fit
Line.
6. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the stopping means
Including n-type ring I（12）, n-type ring II（13）, wherein n-type ring I（12）It is installed vertically on lid（5）Upper surface is close to through hole（6）'s
Opening position, n-type ring II（13）It is installed vertically on the opening position close to switching knob.
7. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1 or 6, it is characterised in that refrigerating plant
Lower surface be provided with improved mat（14）.
8. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the splendid attire vessel
2 include the kettle shell made of double-layer stainless steel material, are provided with heat-insulation layer in kettle shell, the back side of kettle shell is provided with
For the suspension member that splendid attire vessel are hung on metope or support frame（15）.
9. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1 or 8, it is characterised in that the splendid attire
Vessel（2）Side be provided be used for hang liquid nitrogen output pipe（3）Support（16）.
10. according to a kind of liquid nitrogen grinding device for being used to extract RNA described in claim 1, it is characterised in that the mortar（1）
Height be 8-10 centimetres, mortar（1）Higher than 2-4 centimetres of refrigerating plant upper surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720561113.8U CN206778636U (en) | 2017-05-19 | 2017-05-19 | A kind of liquid nitrogen grinding device for being used to extract RNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720561113.8U CN206778636U (en) | 2017-05-19 | 2017-05-19 | A kind of liquid nitrogen grinding device for being used to extract RNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206778636U true CN206778636U (en) | 2017-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720561113.8U Expired - Fee Related CN206778636U (en) | 2017-05-19 | 2017-05-19 | A kind of liquid nitrogen grinding device for being used to extract RNA |
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| Country | Link |
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| CN (1) | CN206778636U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801732A (en) * | 2018-06-15 | 2018-11-13 | 王阳 | A kind of cryogenic sample crushing device |
-
2017
- 2017-05-19 CN CN201720561113.8U patent/CN206778636U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801732A (en) * | 2018-06-15 | 2018-11-13 | 王阳 | A kind of cryogenic sample crushing device |
| CN108801732B (en) * | 2018-06-15 | 2021-01-12 | 李建锋 | Low temperature sample crushing device |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171222 |
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| CF01 | Termination of patent right due to non-payment of annual fee |