CN206684042U - The microscope mutually amplified based on lock - Google Patents
The microscope mutually amplified based on lock Download PDFInfo
- Publication number
- CN206684042U CN206684042U CN201720393278.9U CN201720393278U CN206684042U CN 206684042 U CN206684042 U CN 206684042U CN 201720393278 U CN201720393278 U CN 201720393278U CN 206684042 U CN206684042 U CN 206684042U
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- China
- Prior art keywords
- microscope
- lock
- amplifier
- light source
- connector
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The utility model discloses a kind of microscope mutually amplified based on lock, the microscope includes light source and photoswitch, wherein the photoswitch includes at least one of mechanical switch, chopper and acousto-optic modulator AOM；The microscope also includes lock-in amplifier and/or computer, it is synchronous with the photoswitch during using the lock-in amplifier and/or computer regulation photodetector gathered data, then lock-in amplifier and/or computer process signal are utilized, useful signal is amplified, suppress noise signal, wherein, the photodetector includes camera and/or photomultiplier.The utility model compared with prior art, in the case where not changing microscope this body structure, can improve the resolution ratio and signal to noise ratio of image, while improve microscopical adaptability, and be not limited under conditions of darkroom and gather image.
Description
Technical field
It the utility model is related to a kind of microscope, and in particular to a kind of microscope with lock phase enlarging function.
Background technology
Microscope, the narrow band light usually sent with certain light source (such as laser) or the wide spectrum by narrow band pass filter
Light is irradiated on sample as exciting light, the fluorescence that excites sample itself, the fluorescence for being fluorescently labeled sample, non-marked
The Raman diffused light of sample, it is amplified and carries out after a series of filter systems, then by object lens and eyepiece or camera
Observation, using different filter systems, we can obtain fluoroscopic image, Raman image, scattered light image, second harmonic respectively
(as used short-pulse laser).The microscope can be used for the research absorption of intracellular matter, transport, the distribution of chemical substance and
Positioning, the cellular morphology of tissue and changing rule etc..It is to carry out biology, cytology, materialogy, science of heredity, immunology, pharmacy
Etc. the important instrument of research field.
Microscopical essential structure be have object lens, eyepiece (or by the replacement of the detector such as CCD, PMT) and lighting source and
The devices such as some annexes (such as optical filter, dichroscope, electrooptical switching) form.It is (filtered that its light source is generally high-pressure sodium lamp
Mating plate group, obtain a series of monochromatic light) or multiple wavelength laser as on light irradiation to sample.Except Rayleigh scattering
In addition, other useful flashlights are all weaker than exciting light, in addition to observing sharp keen scattering, it would be desirable to utilize specific optical filter
Go to observe the signals such as special fluorescence, Raman, second harmonic, while prevent exciting light from entering eyepiece or detector, avoid eye
The damage of eyeball or detector.
Advanced microscope producer of the world today includes Olympus, Nikon, Lycra, Zeiss etc., and the light source of use is main
It is xenon lamp, Halogen lamp LED, high-pressure sodium lamp or laser, high-end microscope even uses femto-second laser, for exciting sample
The flashlights such as the fluorescence of different wave length, Raman diffused light, second harmonic.But the intensity of the flashlight of some samples is especially micro-
Much bigger than the intensity of flashlight of especially fast or ambient light are quenched in weak, fluorescence signal so that we can not accurately obtain sample
Real information.
Utility model content
The utility model provides a kind of low cost and improves image intensity and resolution ratio without changing light source can
Microscope.
The technical solution of the utility model：
A kind of microscope mutually amplified based on lock, include the microscope, photoswitch 2 and control process system of light source；
Described photoswitch 2 is arranged between light source 1 and the microscope for having light source, enters the microscope for having light source for modulating
Interior light；
Described control process system is lock-in amplifier and/or computer 14；
The first scheme：When control process system only includes lock-in amplifier, first connector of lock-in amplifier
It is connected with photoswitch 2, for controlling the folding of photoswitch 2, and then controls the light for entering and having in the microscope of light source；Lock mutually amplifies
Second connector of device is connected with eyepiece or backwards to detector 7, or second connector and forward detection of lock-in amplifier
Device 13 connects；Or second connector of lock-in amplifier and eyepiece or be connected backwards to detector 7, while the of lock-in amplifier
Three connectors are connected with forward detection device 13；
Second scheme：When control process system includes computer, computer replaces lock-in amplifier；
The third scheme：When control process system includes lock-in amplifier and computer, first company of lock-in amplifier
Interface is connected with photoswitch 2, for controlling the folding of photoswitch 2, and then controls the light for entering and having in the microscope of light source；Lock phase
Second connector of amplifier is connected with eyepiece or backwards to detector 7, or second connector and forward direction of lock-in amplifier
Detector 13 connects；Or second connector of lock-in amplifier is connected with eyepiece or backwards to detector 7, while lock-in amplifier
The 3rd connector be connected with forward detection device 13；4th connector of lock-in amplifier is connected with computer.
It is synchronous with the photoswitch using being needed when carrying out data acquisition backwards to detector or forward detection device, after by locking
Phase amplifier and/or computer process signal, useful signal is extracted, suppress noise signal and ambient light signal.
Described photoswitch is mechanical switch, chopper or acousto-optic modulator；
The light source is LASER Light Source and/or continuous light source.
The described microscope for having light source is flying-spot microscope, fluorescence microscope, scanning fluorescence microscope or copolymerization Jiao are swept
Retouch microscope.
Described includes camera and/or photomultiplier backwards to detector or forward detection device.
The utility model is first with shutter (mechanical shutter, chopper or AOM (acousto-optic modulator)) device to incidence
Light is modulated before irradiating to sample so that it becomes the incident light changed with certain frequency f；Secondly, when this incidence
After light is irradiated onto sample, flashlight (including Rayleigh scattering light, fluorescence, second harmonic) of electromagnetic radiation etc. also will be with f frequencies
Rate is to external radiation；Flashlight is received by detectors such as CCD or PMT again, and it will pass through lock-in amplifier or computer during reception
The shutter of detector is modulated, makes it identical with frequency f before and phase.Finally in lock-in amplifier or computer
The signal obtained when shutter is opened subtracts signal when shutter closes so that useful signal is amplified, and noise of instrument or detection
Arriving for the garbage signals such as device noise suppresses, and improves signal to noise ratio.
Therefore, the utility model need not be transformed original device structure and device, only on the basis of original,
A shutter device is added in light source part, while carries out certain synchronization and processing can in the detection phase using software and carries
The indexs such as the resolution ratio of high instrument, sweep speed, sensitivity, reduce improvement cost.
Meanwhile inhibitory action may also function as to ambient light based on the microscope that lock mutually amplifies.It is well known that because ambient light
Wavelength hand over wide, also there is certain frequency sometimes.Microscope many times needs to be operated in the environment in darkroom, to avoid environment
Influence of the light to image, or even the light of display all can have large effect, it is necessary to which display closing is adopted to image sometimes
Collect image.And the utility model can be same in the case where not obtaining dark room conditions under dark room conditions or not image resolution ratio.
The beneficial effects of the utility model：On the basis of original scan is microscopical, shutter is arranged on exciting light to sample
In light path between product, exciting light is modulated, in scanning process, original signal acquisition modulated；Then, will
Each pixel of photodetector will be divided into even signal in original scan, will be visited using lock-in amplifier or computer
Survey the frequency and phase-modulation and shutter synchronization of device.The signal arrived that finally shutter is closed using lock-in amplifier or computer and
The signal that an adjacent shutter is opened makes the difference, and then integrates, and obtains the final signal of this point.
Brief description of the drawings
Fig. 1 is the microscopic structure schematic diagram of the present utility model mutually amplified based on lock.
Fig. 2 is the structural representation of flying-spot microscope involved in the microscope of the present utility model mutually amplified based on lock
Figure.
In figure：1 light source；2 photoswitches；3 collimating and beam expanding systems；4x is to scanning galvanometer；5y is to scanning galvanometer；6 dichroscopes；
7 eyepieces or backwards detector；8 object lens；9 samples；10 speculums；11 optical filters；12 convex lens；13 forward detection devices；14 lock phases
Amplifier and/or computer.
Embodiment
Below in conjunction with accompanying drawing and technical scheme, specific embodiment of the present utility model is further illustrated.
As shown in Figure 1-2, it is of the present utility model based on lock phase zoom microscope some embodiments in, only x to
Scanning galvanometer 4 and y have any different to scanning galvanometer 5 in simple microscope and flying-spot microscope, and other functions are essentially identical.Light source
1 sends exciting light, and exciting light is become into the larger directional light of hot spot by beam-expanding collimation system 3, reflects and swashs by dichroscope 6
Light to object lens 8, sample 9 focused on through object lens 8, the flashlight that sample 9 is sent can be divided into two parts, a part return object lens 8,
Eyepiece is reached through dichroscope 6 or backwards to detector 7；Another part can reflect through sample 9 through reflective mirror 10, optical filter 11
Filter, convex lens 12 reach the collection that forward detection device 13 completes signal after focusing on.The utility model adds on excitation light path
Photoswitch 2, it is then by lock-in amplifier or computer 14 that photoswitch and photodetector is synchronous, by each locus
Signal is divided into even number, and wherein odd number is the signal that detector 7 and/or 13 obtains when photoswitch 3 is closed, and even number is photoswitch
The signal obtained when opening, then handled by lock-in amplifier and/or computer 14 so that useful signal is amplified, nothing
Signal is suppressed, so as to realize the raising of picture quality.
Therefore, compared with existing microscopy, its improvement cost is low, and need not change the structure inside microscope
Existing light source need not more be changed, it is possible to improve image quality, while solve ambient light to microscopical influence, extension
Existing microscopical use range.
Above-described is only preferred embodiment of the present utility model, it is noted that for those skilled in the art
For, on the premise of not departing from the utility model and creating design, some modification and improvement can also be made, these are belonged to
The scope of protection of the utility model.
Claims (10)
1. a kind of microscope mutually amplified based on lock, it is characterised in that the microscope mutually amplified based on lock includes light
Microscope, the photoswitch in source（2）With control process system；
Described photoswitch（2）It is arranged on light source（1）And between having the microscope of light source, enter the microscope for having light source for modulating
Interior light；
Described control process system is lock-in amplifier and/or computer（14）；
When control process system only includes lock-in amplifier, first connector and photoswitch of lock-in amplifier（2）Connection,
For controlling photoswitch（2）Folding, and then control to enter and have light in the microscope of light source；Second company of lock-in amplifier
Interface is with eyepiece or backwards to detector（7）Connection, or second connector of lock-in amplifier and forward detection device（13）Connection；
Or second connector of lock-in amplifier is with eyepiece or backwards to detector（7）Connection, while the 3rd company of lock-in amplifier
Interface and forward detection device（13）Connection；
When control process system only includes computer, first connector and photoswitch of computer（2）Connection, for controlling light to open
Close（2）Folding, and then control to enter and have light in the microscope of light source；Second connector of computer is with eyepiece or backwards to spy
Survey device（7）Connection, or second connector of computer and forward detection device（13）Connection；Or second connector and mesh of computer
Mirror or backwards detector（7）Connection, while the 3rd connector of computer and forward detection device（13）Connection；When control process system
When system includes lock-in amplifier and computer, first connector and photoswitch of lock-in amplifier（2）Connection, for controlling light to open
Close（2）Folding, and then control to enter and have light in the microscope of light source；Second connector of lock-in amplifier and eyepiece or
Backwards to detector（7）Connection, or second connector of lock-in amplifier and forward detection device（13）Connection；Or lock-in amplifier
Second connector and eyepiece or backwards to detector（7）Connection, while the 3rd connector of lock-in amplifier is visited with forward direction
Survey device（13）Connection；4th connector of lock-in amplifier is connected with computer.
2. the microscope according to claim 1 mutually amplified based on lock, it is characterised in that using backwards to detector or forward direction
When detector carries out data acquisition, backwards to detector and forward detection device（13）With described photoswitch（2）Synchronously, by lock after
Phase amplifier and/or computer process signal, useful signal is extracted, suppress noise signal and ambient light signal.
3. the microscope according to claim 1 or 2 mutually amplified based on lock, it is characterised in that described photoswitch（2）For
Mechanical switch, chopper or acousto-optic modulator.
4. the microscope according to claim 1 or 2 mutually amplified based on lock, it is characterised in that the light source is laser light
Source and/or continuous light source.
5. the microscope according to claim 1 or 2 mutually amplified based on lock, it is characterised in that the light source is laser light
Source and/or continuous light source.
6. the microscope according to claim 1 or 2 mutually amplified based on lock, it is characterised in that described has the aobvious of light source
Micro mirror is flying-spot microscope, fluorescence microscope, scanning fluorescence microscope or confocal scanning microscope.
7. the microscope according to claim 3 mutually amplified based on lock, it is characterised in that the described microscope for having light source
For flying-spot microscope, fluorescence microscope, scanning fluorescence microscope or confocal scanning microscope.
8. the microscope according to claim 4 mutually amplified based on lock, it is characterised in that the described microscope for having light source
For flying-spot microscope, fluorescence microscope, scanning fluorescence microscope or confocal scanning microscope.
9. the microscope mutually amplified based on lock according to claim 1,2,7 or 8, it is characterised in that described visits backwards
Surveying device or forward detection device includes camera and/or photomultiplier.
It is 10. according to claim 6 based on the microscope that mutually amplifies of lock, it is characterised in that it is described backwards to detector or
Forward detection device includes camera and/or photomultiplier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720393278.9U CN206684042U (en) | 2017-04-17 | 2017-04-17 | The microscope mutually amplified based on lock |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720393278.9U CN206684042U (en) | 2017-04-17 | 2017-04-17 | The microscope mutually amplified based on lock |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206684042U true CN206684042U (en) | 2017-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720393278.9U Expired - Fee Related CN206684042U (en) | 2017-04-17 | 2017-04-17 | The microscope mutually amplified based on lock |
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| Country | Link |
|---|---|
| CN (1) | CN206684042U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932374A (en) * | 2017-04-17 | 2017-07-07 | 大连理工大学 | Based on the microscope that lock mutually amplifies |
| WO2021089010A1 (en) * | 2019-11-07 | 2021-05-14 | 中国科学院深圳先进技术研究院 | Optical microscopic device for focal point modulation on basis of resonant galvanometer, and method |
-
2017
- 2017-04-17 CN CN201720393278.9U patent/CN206684042U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106932374A (en) * | 2017-04-17 | 2017-07-07 | 大连理工大学 | Based on the microscope that lock mutually amplifies |
| WO2021089010A1 (en) * | 2019-11-07 | 2021-05-14 | 中国科学院深圳先进技术研究院 | Optical microscopic device for focal point modulation on basis of resonant galvanometer, and method |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171128 Termination date: 20200417 |
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| CF01 | Termination of patent right due to non-payment of annual fee |