CN206680468U - A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell - Google Patents
A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell Download PDFInfo
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- CN206680468U CN206680468U CN201720402457.4U CN201720402457U CN206680468U CN 206680468 U CN206680468 U CN 206680468U CN 201720402457 U CN201720402457 U CN 201720402457U CN 206680468 U CN206680468 U CN 206680468U
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- driven shaft
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Abstract
The utility model discloses a kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell, including bearing block, driven shaft, bottom plate and brushless electric machine, the bearing block and brushless electric machine are arranged on both sides above bottom plate, the driven shaft is arranged in bearing block, the brushless electric machine front end is provided with the drive shaft set in external screw thread, the drive shaft front end is provided with the sleeve pipe set in internal thread, described sleeve pipe is threadedly coupled with drive shaft, on the inside of described sleeve pipe dividing plate is welded with close to drive shaft one end, the dividing plate side is welded with pole, the blade of helically shape is welded with the outside of the pole, described sleeve pipe front end is welded with the collar, neck is set on the inside of the collar, the driven shaft is provided with fixture block close to one end of the collar, the fixture block is connected with neck clamping, described sleeve pipe is provided with rubber stopper close to one end of driven shaft.The separation preparation facilities structural stability of the human umbilical tissue mescenchymal stem cell is high, easy to operate, easy assembly and disassembly.
Description
Technical field
It the utility model is related to a kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell.
Background technology
Umbilical cord mesenchymal stem cells refer to be present in a kind of versatile stem cell in neonatal umbilical cord tissue, and it can break up
Into many kinds of histocytes, there is wide potential applicability in clinical practice.Umbilical cord mesenchymal stem cells are based on human cord blood serum
The method of the umbilical cord mesenchymal stem cells culture amplification of the cultivating system of body：Flow cytomery result shows, attached cell
CD44, CD29 are expressed, low expression CD106, does not express hematopoietic cell phenotype CD14, CD34, CD45 and endothelial cell phenotype
CD31, also not expression of HLA-DR：Cell doubling time is 30h, and cell cycle analysis show, shared by G0~G1 phases and+G2+M phases
Ratio is respectively 78.84% and 11.16%.Conclusion：Can Successful amplification human umbilical cord mesenchymal using inactivation cord serum cultivating system
Stem cell, the cell of culture have the fundamental characteristics of mescenchymal stem cell, are carried to establish mesenchyma stem cell and clinical practice
Theoretical foundation is supplied.
Umbilical cord mesenchymal stem cells（MSCs）With higher differentiation potential, can be broken up to multiple directions.It bone,
There is wide potential applicability in clinical practice in terms of the organizational projects such as cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscle.Have
Report isolates MSCs from people's umbilical cord, and cell content, multiplication capacity are better than bone marrow MSCs, and immunogenicity compares bone marrow MSCs
It is low, and there is convenient material drawing, the advantages that no ethics dispute, therefore increasingly by the concern of research workers.It is dry thin
Mainly application contains animal blood serum to the cultivating system of born of the same parents（Such as hyclone）Culture medium, the stem cell grown under such a environment,
Its internal structure can occur which kind of change it is on the knees of the gods, to avoid the pathogen contamination such as virus and xenogenesis in culture containing animal blood serum
Allergic reaction caused by serum, seminar have carried out the cultivating system cultured and amplified in vitro umbilical cord based on human cord blood serum
The experimental study of mescenchymal stem cell.It is dry for cultivating clinical tissue engineering use to inquire into human cord blood serum's replacement animal blood serum
The feasibility of cell.The characteristics such as umbilical cord mesenchymal stem cells morphology, growth characteristics, cell cycle, immunophenotype are studied simultaneously,
It is intended to establish the enrichment method of umbilical cord mesenchymal stem cells.
, it is necessary to use preparation facilities in the extraction process of human umbilical tissue mescenchymal stem cell, but existing system
Standby apparatus structure stability is not high, inconvenient for operation, it is not easy to assembly and disassembly.
The content of the invention
In view of this, the utility model purpose is to provide a kind of structural stability height, easy to operate, easy assembly and disassembly
Human umbilical tissue mescenchymal stem cell separation preparation facilities.
In order to solve the above-mentioned technical problem, the technical solution of the utility model is：
A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell, including bearing block, driven shaft, bottom plate and brushless
Motor, the bearing block and brushless electric machine are arranged on both sides above bottom plate, and the driven shaft is arranged in bearing block, described brushless
Motor front end is provided with the drive shaft set in external screw thread, and the drive shaft front end is provided with the sleeve pipe set in internal thread, institute
State sleeve pipe to be threadedly coupled with drive shaft, dividing plate, the dividing plate side welding are welded with close to drive shaft one end on the inside of described sleeve pipe
There is pole, the blade of helically shape is welded with the outside of the pole, described sleeve pipe front end is welded with the collar, the collar inner side
Neck is set, and the driven shaft is provided with fixture block close to one end of the collar, and the fixture block is connected with neck clamping, and described sleeve pipe is close
One end of driven shaft is provided with rubber stopper.
Preferably, being provided with axle sleeve between the driven shaft and bearing block, axle sleeve can reduce the abrasion of driven shaft.
Preferably, the axle sleeve connects with bearing block interference fit, axle sleeve can rotate in bearing block.
Preferably, the driven shaft is connected with axle sleeve gap so that driven shaft can move horizontally in axle sleeve.
Preferably, described sleeve pipe is stainless steel sleeve pipe, corrosion-resistant, service life length.
Preferably, being inlaid with glass bar in the middle part of described sleeve pipe, glass bar is made of safety glass.
Preferably, the glass bar is connected with bobbin seal, glass bar carries out close with cannula connection smearing epoxy glue
Envelope.
The utility model has the technical effect that following aspect：The bearing block and brushless electric machine of setting are arranged on bottom plate
Top both sides, driven shaft are arranged in bearing block, and bearing block causes driven shaft slow-roll stabilization so that structural stability is high；Set
Sleeve pipe rubber stopper is installed close to one end of driven shaft, rubber stopper conveniently takes out the raw material in sleeve pipe, keeps easy to operate；If
The sleeve pipe put is threadedly coupled with drive shaft, sets neck on the inside of the collar, and driven shaft is provided with fixture block close to one end of the collar, fixture block and
Neck clamping connects so that the easy assembly and disassembly of sleeve pipe.
Brief description of the drawings
Fig. 1 is a kind of structure chart of the separation preparation facilities of human umbilical tissue mescenchymal stem cell of the utility model.
Fig. 2 is a kind of sleeve pipe profile of the separation preparation facilities of human umbilical tissue mescenchymal stem cell of the utility model.
Embodiment
Below in conjunction with accompanying drawing, specific embodiment of the present utility model is described in further detail, so that the utility model skill
Art scheme is more readily understood and grasped.
As shown in Figure 1-2, a kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell, including it is bearing block 1, driven
Axle 2, bottom plate 3 and brushless electric machine 4, the concrete model of brushless electric machine 4 are 57BL52-212.The bearing block 1 and brushless electric machine 4 are installed
The both sides above bottom plate 3, bearing block 1 and brushless electric machine 4 are welded and fixed with bottom plate 3.The driven shaft 2 is arranged in bearing block 1,
Axle sleeve 14 is installed between the driven shaft 2 and bearing block 1, axle sleeve 14 can reduce the abrasion of driven shaft 2.The axle sleeve 14 with
The interference fit connection of bearing block 1, axle sleeve 14 can rotate in bearing block 1.The driven shaft 2 coordinates with the gap of axle sleeve 14 and connected
Connect so that driven shaft 2 can move horizontally in axle sleeve 14.The front end of brushless electric machine 4 is provided with the drive set in external screw thread
Moving axis 5, the front end of drive shaft 5 are provided with the sleeve pipe 6 set in internal thread, and described sleeve pipe 6 is threadedly coupled with drive shaft 5, institute
It is stainless steel sleeve pipe to state sleeve pipe 6, corrosion-resistant, service life length.
The middle part of described sleeve pipe 6 is inlaid with glass bar 15, and glass bar 15 is made of safety glass.The glass bar 15 and set
Pipe 6 is tightly connected, and glass bar 15 is smeared epoxy glue with the junction of sleeve pipe 6 and sealed, and glass tube 15 is convenient to entering inside sleeve pipe 6
Row observation.The inner side of described sleeve pipe 6 is welded with dividing plate 7 close to the one end of drive shaft 5, and the side of dividing plate 7 is welded with pole 8, described
The outside of pole 8 is welded with the blade 9 of helically shape, and the front end of described sleeve pipe 6 is welded with the collar 10, and the inner side of the collar 10 is set
Neck 11, the driven shaft 2 are provided with fixture block 12 close to one end of the collar 10, and the fixture block 12 is connected with the clamping of neck 11, described
Sleeve pipe 6 is provided with rubber stopper 13 close to one end of driven shaft 2.
When in use, human umbilical tissue is placed in sleeve pipe 6, starts brushless electric machine 4 and sleeve pipe 6 is driven by drive shaft 5（Sleeve pipe
6 is consistent with the steering of drive shaft 5 with the direction that the screw thread of drive shaft 5 screws, and such retaining sleeve 6 will not slip）Rotate, pole 8
The blade 9 of the helically shape of outside welding can carry out broken homogeneous to human umbilical tissue, close brushless electric machine 4, will be driven
Axle 2 is removed in the horizontal direction, you can rotating casing 6 is removed from drive shaft 5, and it is the liquid that can be taken off homogeneous to open rubber stopper 13
Body, stratification can the isolated liquid containing stem cell.
The utility model has the technical effect that following aspect：The bearing block and brushless electric machine of setting are arranged on bottom plate
Top both sides, driven shaft are arranged in bearing block, and bearing block causes driven shaft slow-roll stabilization so that structural stability is high；Set
Sleeve pipe rubber stopper is installed close to one end of driven shaft, rubber stopper conveniently takes out the raw material in sleeve pipe, keeps easy to operate；If
The sleeve pipe put is threadedly coupled with drive shaft, sets neck on the inside of the collar, and driven shaft is provided with fixture block close to one end of the collar, fixture block and
Neck clamping connects so that the easy assembly and disassembly of sleeve pipe.
Certainly, it is representative instance of the present utility model above, in addition, the utility model can also have other a variety of
Embodiment, all technical schemes formed using equivalent substitution or equivalent transformation, all falls within the requires of the utility model protection
Within the scope of.
Claims (7)
- A kind of 1. separation preparation facilities of human umbilical tissue mescenchymal stem cell, it is characterised in that：Including bearing block, driven shaft, Bottom plate and brushless electric machine, the bearing block and brushless electric machine are arranged on both sides above bottom plate, and the driven shaft is arranged on bearing block Interior, the brushless electric machine front end is provided with the drive shaft set in external screw thread, and the drive shaft front end is provided with to be set in internal thread The sleeve pipe put, described sleeve pipe are threadedly coupled with drive shaft, are welded with dividing plate close to drive shaft one end on the inside of described sleeve pipe, it is described every Plate side is welded with pole, and the blade of helically shape is welded with the outside of the pole, and described sleeve pipe front end is welded with the collar, institute State and neck is set on the inside of the collar, the driven shaft is provided with fixture block close to one end of the collar, and the fixture block is connected with neck clamping, institute State sleeve pipe and rubber stopper is installed close to one end of driven shaft.
- 2. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 1, it is characterised in that：It is described Axle sleeve is installed between driven shaft and bearing block.
- 3. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 2, it is characterised in that：It is described Axle sleeve connects with bearing block interference fit.
- 4. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 2, it is characterised in that：It is described Driven shaft is connected with axle sleeve gap.
- 5. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 4, it is characterised in that：It is described Sleeve pipe is stainless steel sleeve pipe.
- 6. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 1, it is characterised in that：It is described Glass bar is inlaid with the middle part of sleeve pipe.
- 7. the separation preparation facilities of human umbilical tissue mescenchymal stem cell according to claim 6, it is characterised in that：It is described Glass bar is connected with bobbin seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720402457.4U CN206680468U (en) | 2017-04-18 | 2017-04-18 | A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720402457.4U CN206680468U (en) | 2017-04-18 | 2017-04-18 | A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell |
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| Publication Number | Publication Date |
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| CN206680468U true CN206680468U (en) | 2017-11-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720402457.4U Active CN206680468U (en) | 2017-04-18 | 2017-04-18 | A kind of separation preparation facilities of human umbilical tissue mescenchymal stem cell |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109998601A (en) * | 2019-04-25 | 2019-07-12 | 河南卓丰生物科技有限公司 | A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue |
-
2017
- 2017-04-18 CN CN201720402457.4U patent/CN206680468U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109998601A (en) * | 2019-04-25 | 2019-07-12 | 河南卓丰生物科技有限公司 | A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue |
| CN109998601B (en) * | 2019-04-25 | 2021-07-20 | 河南卓丰生物科技有限公司 | A supplementary stripping off device that is used for human umbilical cord periphery huatong glue |
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