CN206638663U - A kind of zebra fish temperature-sensitivbehavior behavior liveness detector - Google Patents

A kind of zebra fish temperature-sensitivbehavior behavior liveness detector Download PDF

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Publication number
CN206638663U
CN206638663U CN201720450124.9U CN201720450124U CN206638663U CN 206638663 U CN206638663 U CN 206638663U CN 201720450124 U CN201720450124 U CN 201720450124U CN 206638663 U CN206638663 U CN 206638663U
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CN
China
Prior art keywords
pond
water tank
camera device
zebra fish
thermostat
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Expired - Fee Related
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CN201720450124.9U
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Chinese (zh)
Inventor
付先军
彭维兵
王振国
李学博
于华芸
周玲晓
陈聪
李佼阳
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Shandong University of Traditional Chinese Medicine
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Shandong University of Traditional Chinese Medicine
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Abstract

The utility model discloses a kind of zebra fish temperature-sensitivbehavior behavior liveness detector, including water tank and camera device;The water tank includes at least two thermostat modules, and at least provided with three ponds in each thermostat module, the pond in different thermostat modules is separate, and water temperature is different;The upper opening in pond, camera device are arranged at the upper end of water tank, and camera is set towards the opening direction in pond.Pond in different thermostat modules is separate, and the water environment for making different warm areas is relative closure, will not produce the flowing of water and influence accurate temperature controlling.Camera device is set in the top in pond, camera can record the liveness of zebra fish in pond, and then can react influence of the Chinese medicine micro constitutent fever and chills property of medicine to zebra fish liveness.

Description

A kind of zebra fish temperature-sensitivbehavior behavior liveness detector
Technical field
The utility model is specifically related to a kind of zebra fish temperature-sensitivbehavior behavior liveness detector.
Background technology
There are the devices such as rat fever and chills approach behavior monitoring to the objective characterisation pattern of the Chinese medicinal material extract property of medicine at present, But these devices can not record the movement track at a temperature of animal varying environment and be analyzed, it is impossible to observe Chinese medicine micro constitutent Fever and chills effect, the material base of the Chinese medicine fever and chills property of medicine can not be verified.Zebra fish is kind of a model organism, due to the gene of zebra fish Reach 87% with the similarity of human gene, this means that does result obtained by drug study as a rule with it It is also applied for human body.Moreover, the embryo of zebra fish is transparent, so being readily observed shadow of the medicine to its intracorporeal organ Ring.In addition, female zebra fish can lay eggs 200 pieces, embryo can develop shaping in 24h, and this allows in the same generation fish body It is upper to carry out different experiments.The medication of zebra fish is micro, has some superiority for the drug efficacy study of Chinese medicine micro constitutent.
Although having had zebra fish temperature-sensitivbehavior action trail monitoring device to record the action trail of zebra fish, can not remember The active degree of zebra fish behavior is recorded, can not such as contrast work during zebra fish feeding different properties traditional Chinese medicine ingredients at the same temperature Jerk, the active degree of the zebra fish of the identical property of medicine traditional Chinese medicine ingredients of feeding at different temperatures can not be also contrasted, and then can not examined Survey influence of the fever and chills property of medicine of Chinese medicine micro constitutent to zebra fish liveness.Meanwhile test where zebra fish in water tank not equality of temperature The flowing of degree section water easily causes the unstable of water temperature and influences result.
Utility model content
For above-mentioned problems of the prior art, the utility model provides a kind of zebra fish temperature-sensitivbehavior behavior liveness inspection Survey instrument.Water tank is divided into different constant temperature sections, multiple water is set in each constant temperature section by the detector using principle is split Pond, the zebra fish of feeding different properties traditional Chinese medicine ingredients is placed respectively in the pond in each constant temperature section, in observing and record Influence of the fever and chills property of medicine of medicine micro constitutent to the liveness of zebra fish;It is identical that feeding is placed in the pond in different constant temperature sections The zebra fish of traditional Chinese medicine ingredients, it is comprehensive to observe and record influence of this kind of traditional Chinese medicine ingredients at different temperatures to zebra fish liveness Close influence of the fever and chills property of medicine of analysis traditional Chinese medicine ingredients to zebra fish liveness, and then the fever and chills medicine of reasonable prediction Chinese medicine micro constitutent Influence of the property to human body.
In order to solve the above technical problems, the technical solution of the utility model is:
A kind of zebra fish temperature-sensitivbehavior behavior liveness detector, including water tank and camera device;The water tank includes at least two Individual thermostat module, at least provided with three ponds in each thermostat module, and temperature control module is provided with each thermostat module, Temperature control module includes the multiple temperature sensors being respectively arranged in different ponds, the multiple heating heated respectively to different ponds Device and controller, temperature sensor and heater are connected with controller;The upper opening in pond, camera device are arranged at water tank Upper end, the camera of camera device sets towards the opening direction in pond.
Pond in different thermostat modules is separate, and the water environment for making different warm areas is relative closure, will not produce The flowing of water and influence accurate temperature controlling.Because the liveness of the zebra fish of feeding different properties traditional Chinese medicinal components is different, so easily Cause the lost speed of heat of the pond reclaimed water in same thermostat module different, it is right respectively herein by the multiple heaters of setting Different ponds are heated, and ensure that the water temperature in same thermostat module in pond keeps constant.
Camera device is set in the top in pond, camera can record the liveness of zebra fish in pond, and then can be with React influence of the Chinese medicine micro constitutent fever and chills property of medicine to zebra fish liveness.
Further, it is interconnected in same thermostat module between pond, connectivity part is provided with netted obstacles.
Pond in same thermostat module is interconnected, temperature is identical between may further ensure that pond, netted resistance Block thing zebra fish is limited in respective scope of activities, be easy to observe and shoot, to avoid confusion.
Further, it is provided with thermal baffle between adjacent thermostat module.Thermal baffle can prevent different temperatures pond Between temperature transmission, further prevent the transmission that heat occurs between the water of different temperatures, be easy to the accurate control of temperature.
Further, it is separate structure between the pond and water tank, pond is cylindrical container, the opening of cylindrical container End is provided with flange, and cylindrical groove is provided with water tank, and cylindrical container is positioned in cylindrical groove, and the bottom of cylindrical groove is set Heater.
After experiment, cylindrical container can be carried away by the top flange of cylindrical container, directly pull zebra fish out And outwell water, when avoiding the direct operation in water tank, the water spilt produces not to water in tank parts or other ponds Profit influences.
Further, the camera device is arranged on the top of water tank by support, and the both sides of support are separately fixed at water On two relative sides of case.
Camera device is fixed on above water tank by support, and support is fixed on water tank, makes water tank, camera device and support As an entirety, it is easy to manufacture and moves.
Further, the support is portal trestle, including the vertical pole of both sides and is fixed on two vertical poles The cross bar at end, the camera device are arranged on the cross bar.
Further, the height of two of the support vertical poles is identical, and the camera device is movably arranged on institute State on cross bar, realize camera device and moved along the cross bar.
Because water tank includes different thermostat modules, so water tank has certain length and width, if camera device is fixed In same place, then it is difficult to the behavior situation to the zebra fish in each pond and records.By the way that camera device activity is installed, Adjust the position of camera device, you can realize and the behavior situation of zebra fish in different ponds is recorded.
Further, two of the support vertical poles are movably arranged on two relative sides of water tank, make branch Frame realizes the translation along water tank side.
The translation of support is easier to realize the movement to the position of camera device, is easy to preferably record.
Further, two of the support vertical poles are telescopic pole.
Telescopic pole can adjust the height of vertical pole, so adjust camera device relative to pond opening away from From to meet different to shoot with video-corder requirement.
Further, the pond is cylinder.
Further, temperature control system is provided with each thermostat module, temperature control system includes heater, temperature Sensor and controller, temperature sensor are arranged inside pond, and are connected with controller, and controller is connected with heater, institute State the bottom that heater is arranged at pond.
Further, three thermostat modules are provided with the water tank, each thermostat module includes three ponds.
Further, three ponds in each thermostat module are in one line, and the pond in different thermostat modules is parallel Set.
The beneficial effects of the utility model are:
1st, the pond in different thermostat modules is separate, and the water environment for making different warm areas is relative closure, will not produce The flowing of unboiled water and influence accurate temperature controlling.Camera device is set in the top in pond, camera can record zebra fish in pond Liveness, and then influence of the Chinese medicine micro constitutent fever and chills property of medicine to zebra fish liveness can be reacted.
2nd, the position of support and height can be adjusted, and position of the camera device on support can be adjusted, it is possible to achieve be taken the photograph Recording device water tank top position adjustment, in easy water tank in each pond zebra fish behavior record.
Brief description of the drawings
Fig. 1 is the analysis system schematic diagram of zebra fish temperature-sensitivbehavior behavior liveness detector of the present utility model;
Fig. 2 is the structural representation of zebra fish temperature-sensitivbehavior behavior liveness detector of the present utility model;
Fig. 3 is the electric operation control circuit figure of zebra fish temperature-sensitivbehavior behavior liveness detector of the present utility model;
Fig. 4 is zebra fish behavior analysis system architecture figure.
Wherein, 1, camera device, 2, support, 3, pond, 4, thermal baffle, 5, heater, 6, control circuit.
Embodiment
It is noted that described further below is all exemplary, it is intended to provides further instruction to the application.It is unless another Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative It is also intended to include plural form, additionally, it should be understood that, when in this manual using term "comprising" and/or " bag Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
Temperature sensitive behavior:Refer to reaction and Behavioral change caused by the change of temperature.
As shown in figures 1 and 3, analysis system of the present utility model includes temperature control device, multicell closing water environment, can take good care of oneself Recording device and analysis system, analysis process are the temperature in temperature control device adjustment multicell closing water environment, make different temperatures section In water environment temperature it is different, the zebra fish behavior and liveness in corresponding water environment are different.Pass through adjustable camera device The behavior and liveness, the parameter of adjustable camera device for recording zebra fish are adjusted by control circuit 6, and are by analyzing System analysis zebra fish behavior and the influence factor of liveness.
As shown in Fig. 2 zebra fish behavior liveness detector of the present utility model includes water tank and camera device 1;It is described Water tank includes at least two thermostat modules, is provided with three ponds 3 in each thermostat module, three water in each thermostat module Pond 3 is in one line, and the pond 3 in different thermostat modules is be arranged in parallel, and temperature control module, temperature control are provided with each thermostat module Multiple temperature sensors that module includes being respectively arranged in different ponds, multiple heaters that different ponds are heated respectively and Controller, temperature sensor and heater are connected with controller.The upper opening in pond 3, camera device 1 are arranged at water tank Upper end, camera are set towards the opening direction in pond 3, and camera device 1 is arranged on the top of water tank by support 2, support 2 Both sides are separately fixed on two relative sides of water tank.The support 2 is portal trestle, including the vertical pole of both sides and solid The cross bar of two vertical pole upper ends is scheduled on, the height of two vertical poles of the support 2 is identical, the activity peace of camera device 1 On cross bar, realize camera device 1 and moved along cross bar.The vertical pole of two of support is movably arranged on relative two of water tank On individual side, support 2 is set to realize the translation along water tank side.Meanwhile two vertical poles of support 2 are telescopic pole, just In the height of regulation camera device 1.
Camera device 1 is movably arranged on cross bar, and its mode has a variety of, and cross bar such as is arranged into cylindrical bar, shoots with video-corder dress Setting through hole is put, the aperture of through hole can adjust, and be enclosed on cross bar by through hole, it is necessary to during mobile camera device 1, by through hole Aperture tune up, camera device 1 is moved along cross bar, when being moved to certain position, the aperture of through hole turned down, by camera device It is fixed on cross bar.
Such as, groove slide rail is set on cross bar, projection is set on camera device, projection is fastened in groove slide rail, Camera device is movably arranged on cross bar.
The mode that the vertical pole of two of support 2 is movably arranged on water tank side is also a variety of, as long as mesh can be realized .
The zebra fish of feeding different properties traditional Chinese medicine ingredients is placed in the pond 3 of same thermostat module, is centering herein Medicine micro constitutent fever and chills property of medicine effect carries out detection evaluation, so, herein in three ponds of same thermostat module respectively Place the cold composition zebra fish of feeding, the hot composition zebra fish of feeding and normal zebra fish (not feeding traditional Chinese medicine ingredients, blank pair According to), as shown in figure 4, by the video input analysis module of acquisition, transformation assay is carried out by video data conversion module, by counting Corresponding data are exported according to export module.The identical property of medicine traditional Chinese medicine ingredients of feeding can also be placed in the pond of different thermostat modules Zebra fish, to observe and record influence of this kind of traditional Chinese medicine ingredients at different temperatures to zebra fish liveness, in comprehensive analysis Influence of the fever and chills property of medicine of medicine composition to zebra fish liveness.
The preferred embodiment of the application is the foregoing is only, is not limited to the application, for the skill of this area For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.

Claims (10)

  1. A kind of 1. zebra fish temperature-sensitivbehavior behavior liveness detector, it is characterised in that:Including water tank and camera device;The water tank bag At least two thermostat modules are included, are provided with each thermostat module at least provided with three ponds, and each in thermostat module Temperature control module, temperature control module include be respectively arranged in different ponds multiple temperature sensors, different ponds are heated respectively Multiple heaters and controller, temperature sensor and heater are connected with controller;The upper opening in pond, camera device The upper end of water tank is arranged at, the camera of camera device is set towards the opening direction in pond.
  2. 2. detector according to claim 1, it is characterised in that:It is interconnected in same thermostat module between pond, even Logical place is provided with netted obstacles.
  3. 3. detector according to claim 1, it is characterised in that:It is separate structure between the pond and water tank, water Pond is cylindrical container, and the openend of cylindrical container is provided with flange, cylindrical groove is provided with water tank, cylindrical container is positioned over post In connected in star, the bottom of cylindrical groove sets heater.
  4. 4. detector according to claim 1, it is characterised in that:The camera device is arranged on the upper of water tank by support Side, the both sides of support are separately fixed on two relative sides of water tank.
  5. 5. detector according to claim 1, it is characterised in that:The support is portal trestle, including both sides is vertical Pole and the cross bar for being fixed on two vertical pole upper ends, the camera device are arranged on the cross bar.
  6. 6. detector according to claim 5, it is characterised in that:The height of the vertical pole of two of the support is identical, The camera device is movably arranged on the cross bar, is realized camera device and is moved along the cross bar.
  7. 7. detector according to claim 6, it is characterised in that:The vertical pole of two of the support is movably arranged on water On two relative sides of case, support is set to realize the translation along water tank side.
  8. 8. detector according to claim 7, it is characterised in that:The vertical pole of two of the support is telescopic branch Bar.
  9. 9. detector according to claim 1, it is characterised in that:Thermal baffle is provided between different thermostat modules.
  10. 10. detector according to claim 1, it is characterised in that:Three thermostat modules are provided with the water tank, each Thermostat module includes three ponds.
CN201720450124.9U 2017-04-26 2017-04-26 A kind of zebra fish temperature-sensitivbehavior behavior liveness detector Expired - Fee Related CN206638663U (en)

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CN201720450124.9U CN206638663U (en) 2017-04-26 2017-04-26 A kind of zebra fish temperature-sensitivbehavior behavior liveness detector

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Application Number Priority Date Filing Date Title
CN201720450124.9U CN206638663U (en) 2017-04-26 2017-04-26 A kind of zebra fish temperature-sensitivbehavior behavior liveness detector

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111528143A (en) * 2020-05-26 2020-08-14 大连海洋大学 Fish shoal feeding behavior quantification method, system, device and storage medium

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111528143A (en) * 2020-05-26 2020-08-14 大连海洋大学 Fish shoal feeding behavior quantification method, system, device and storage medium
CN111528143B (en) * 2020-05-26 2021-08-20 大连海洋大学 Fish shoal feeding behavior quantification method, system, device and storage medium

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171114

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