CN206627441U - A kind of fluorescent confocal microscopy endoscopic imaging system - Google Patents
A kind of fluorescent confocal microscopy endoscopic imaging system Download PDFInfo
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- CN206627441U CN206627441U CN201720371701.5U CN201720371701U CN206627441U CN 206627441 U CN206627441 U CN 206627441U CN 201720371701 U CN201720371701 U CN 201720371701U CN 206627441 U CN206627441 U CN 206627441U
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Abstract
The utility model is applied to optical microphotograph technical field of imaging, there is provided a kind of fluorescent confocal microscopy endoscopic imaging system, the system include：Fluorescence excitation unit, image acquisition unit, spectrum acquiring unit, fluorescence excitation unit, for exciting testing sample to obtain fluorescence, image acquisition unit, for obtaining confocal fluorescent micro-image, spectrum acquiring unit, for obtaining fluorescence spectrum.The utility model is on the basis of existing fluorescent confocal microscopy endoscopic imaging system, the optics that increase can obtain the spectrometer of fluorescence spectrum information and light path is used cooperatively, to realize fluorescence spectrum with being shown while confocal fluorescence microscope images, solve existing fluorescent confocal microscopy endoscopic imaging system not having while be copolymerized burnt spectral analysis capabilities, when the structure for running into some objects is similar, light intensity detection can not be distinguished the problem of discrimination simultaneously to it.
Description
Technical field
The utility model belongs to optical microphotograph technical field of imaging, more particularly to a kind of fluorescent confocal microscopy endoscopic imaging system
System.
Background technology
Laser scanning co-focusing microscope is one that one that twentieth century grows up the eighties has an epoch-marking significance
Item technology, it is to have installed laser scanning device additional on the basis of fluorescence microscope imaging, and image procossing is carried out using computer,
The resolution ratio of optical imagery improves 30%-40%, and can realize optical sectioning imaging to biological sample etc..Confocal microscopy into
As the light that in system, spot light is sent focuses on sample surfaces after object lens, then after being reflected or transmitted by sample through condenser again
Focus on detector.Aperture three before spot light, image objects face, detector is mutual phase conjugate.By sweep mechanism to sample
Product carry out x/y plane scanning can obtain sweeping for sample many levels to obtain a width two dimensional image of sample, then by axial scan
Trace designs picture, and each tomographic image passes through image procossing, can reconstruct the high-resolution three-dimension tomographic map of sample.
The principle of fluorescent confocal microscopy endoscopic imaging is that laser is passed into internal exposure sample by fibre bundle, to excite sample
Product send fluorescence, then pass fluorescence back through fibre bundle original road, fluorescence are obtained by detector, to be observed, analyzed.Existing skill
The fluorescent confocal microscopy endoscopic imaging system that art provides is copolymerized burnt spectral analysis capabilities while not possessing flashlight, when in use
The spectrum of fluorescence is not often known, is unfavorable for sample similar to some structures, that fluorescent intensity signal is similar and analyzes.
Utility model content
The utility model embodiment provides a kind of fluorescent confocal microscopy endoscopic imaging system, it is intended to solves in the prior art
Fluorescent confocal microscopy endoscopic imaging system do not have the problem of spectral analysis capabilities.
The utility model embodiment provides a kind of fluorescent confocal microscopy endoscopic imaging system, it is characterised in that described glimmering
Light confocal microscopy endoscopic imaging system includes：
Fluorescence excitation unit, image acquisition unit, spectrum acquiring unit；
The fluorescence excitation unit, for exciting testing sample, to obtain fluorescence；
Described image acquiring unit, for obtaining confocal fluorescent micro-image；
The spectrum acquiring unit, for obtaining fluorescence spectrum.
It was found from above-mentioned the utility model embodiment, fluorescent confocal microscopy endoscopic imaging system provided by the utility model,
Including：Fluorescence excitation unit, image acquisition unit, spectrum acquiring unit, fluorescence excitation unit, for exciting testing sample to obtain
Obtain fluorescence, image acquisition unit, for obtaining confocal fluorescent micro-image, spectrum acquiring unit, for obtaining fluorescence spectrum.This
The fluorescent confocal microscopy endoscopic imaging system that utility model provides is the base in existing fluorescent confocal microscopy endoscopic imaging system
On plinth, increase can obtain the spectrometer and the optics that is used cooperatively of light path of fluorescence spectrum information, to realize fluorescence spectrum
With being shown while confocal fluorescence microscope images, solves existing fluorescent confocal microscopy endoscopic imaging system without spectrum point
Analysis ability, when the structure for running into some objects is similar, and light intensity detection can not make a distinction discrimination to it the problem of, meanwhile, this reality
Be advantageous to the commercial application of system with the fluorescent confocal microscopy endoscopic imaging system of new offer.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the utility model, for those skilled in the art, without having to pay creative labor,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation for the fluorescent confocal microscopy endoscopic imaging system that the utility model first embodiment provides.
Embodiment
To enable the purpose of utility model, feature, advantage of the utility model embodiment more obvious and understandable, under
The accompanying drawing that face will be combined in the utility model embodiment, is carried out clear, complete to the technical scheme in the embodiment of the utility model
Ground describes, it is clear that described embodiment is only the utility model part of the embodiment, and not all embodiments.Based on this
Embodiment in utility model, the every other reality that those skilled in the art are obtained under the premise of creative work is not made
Example is applied, belongs to the scope of the utility model protection.
Referring to Fig. 1, Fig. 1 is the knot for the fluorescent confocal microscopy endoscopic imaging system that the utility model first embodiment provides
Structure schematic diagram, for convenience of description, it illustrate only the part related to the utility model embodiment.The fluorescent confocal of Fig. 1 examples
Microscopy endoscopic imaging system, mainly includes：
Fluorescence excitation unit, image acquisition unit, spectrum acquiring unit.
Fluorescence excitation unit, for exciting testing sample to obtain fluorescence；Image acquisition unit, for obtaining confocal fluorescent
Micro-image；Spectrum acquiring unit, for obtaining fluorescence spectrum.
Fluorescence excitation unit, be included in the LASER Light Source 101 being sequentially placed in fluorescence excitation light path, exciter filter 102,
Dichroic mirror 103, the first object lens 104, confocal pinhole 105, the second object lens 106, two-dimensional scanner 107, scanning lens 108, Guan Jing
109th, the 3rd object lens 110, fibre bundle 111, microlens 112.
Image acquisition unit, it is included in confocal fluorescent micro-image and obtains microlens 112, the light being sequentially placed in light path
Fine beam 111, the 3rd object lens 110, pipe mirror 109, scanning lens 108, two-dimensional scanner 107, the second object lens 106, confocal pinhole
105th, the first object lens 104, dichroic mirror 103, transmitting optical filter 113, fluorescence beam splitter 114, the first condenser lens 115, detector
116。
Spectrum acquiring unit, be included in fluorescence spectrum obtain light path on be sequentially placed microlens 112, fibre bundle 111,
3rd object lens 110, pipe mirror 109, scanning lens 108, two-dimensional scanner 107, the second object lens 106, confocal pinhole 105, the first thing
Mirror 104, dichroic mirror 103, transmitting optical filter 113, fluorescence beam splitter 114, the second condenser lens 117, spectrometer 118.
LASER Light Source 101 is used to produce exciting light；
Exciter filter 102 is placed in the light path for the exciting light that LASER Light Source 101 is sent；
Dichroic mirror 103 is placed on exciting light in the light path after exciter filter 102；
First object lens 104 are placed on exciting light in the light path after the transmission of dichroic mirror 103；
Confocal pinhole 105 is placed on exciting light in the light path after the convergence of the first object lens 104；
Second object lens 106 are placed on exciting light in the light path after confocal pinhole 105；
Two-dimensional scanner 107 is placed on exciting light in the light path after the second object lens 106 collimation；
Scanning lens 108 is placed on exciting light in the light path after the two-dimensional scan of two-dimensional scanner 107, two-dimensional scanner
107 are placed on the front focal plane of scanning lens 108；
Pipe mirror 109 is placed in the light path after the scanned lens 108 of exciting light, the back focal plane and Guan Jing of scanning lens 108
109 front focal plane overlaps；
3rd object lens 110 are placed on exciting light in the light path after the collimation of pipe mirror 109；
Fibre bundle 111 is placed on exciting light in the light path after the convergence of the 3rd object lens 110, and exciting light is through the 3rd object lens 110
It is coupled into fibre bundle 111；
Microlens 112 is placed on exciting light in the light path after fibre bundle 111；
Transmitting optical filter 113 is placed on fluorescence in the light path after the reflection of dichroic mirror 103, and fluorescence is treated by excitation
Obtained by test sample product 2；
Fluorescence beam splitter 114 is placed in the emitted 113 filtered light path of optical filter of fluorescence；
First condenser lens 115 is placed on fluorescence in the light path after the transmission of fluorescence beam splitter 114；
Detector 116 is placed on fluorescence after the focusing of the first condenser lens 115 in light path, and detector 116 is placed on first
The back focal plane of condenser lens 115；
Second condenser lens 117 is placed on fluorescence in the light path after the reflection of fluorescence beam splitter 114；
Spectrometer 118 is placed on fluorescence after the focusing of the second condenser lens 117 in light path, and spectrometer 118 is placed on second
The back focal plane of condenser lens 117.
Further, the fluorescent confocal microscopy endoscopic imaging system that the utility model first embodiment provides also includes：Eventually
End 119.
Terminal 119 is electrically connected with two-dimensional scanner 107, detector 116, spectrometer 118 respectively.
Further, the pore size of confocal pinhole 105 can be adjusted, for realizing signal to noise ratio, contrast and resolution ratio
Etc. the regulation of parameter, to strengthen practicality.
Fibre bundle 111 includes multifiber.
The operation principle of the fluorescent confocal microscopy endoscopic imaging system is：
Exciting light is sent by LASER Light Source 101 first, the exciting light can be continuous light or pulsed light, and its wavelength is located at fluorescence
The uptake zone of dyestuff or Auto- fluorescence substance；Afterwards, exciting light filters through exciter filter 102, and the light of target wavelength passes through, its
The light of its wavelength is then blocked, to improve signal to noise ratio；Afterwards, through 102 filtered exciting light of exciter filter through dichroic mirror 103
It is transmitted on the first object lens 104, and is converged to by the first object lens 104 in confocal pinhole 105, confocal pinhole 15 is that pore size can
The confocal pinhole of tune, the regulation of the parameters such as signal to noise ratio, contrast and resolution ratio can be achieved, to strengthen practicality；Afterwards, through confocal
Exciting light after pin hole 105 is emitted is collected and collimated by the second object lens 106, into two-dimensional scanner 107；Afterwards, through two dimension
The scanned lens 108 of exciting light that scanner 107 deflects enter pipe mirror 109 and form collimated light beam, from suitable pipe mirror 109,
So that the diameter of the collimated light beam matches with the aperture of pipe mirror 109, to realize optimum focusing effect, resolution ratio is improved；Afterwards, pass through
Exciting light after pipe mirror 109 collimates enters the 3rd object lens 110, and certain root for being coupled into fibre bundle 111 is assembled by the 3rd object lens 110
In optical fiber, this optical fiber can be designated as current time exciting light incident optical；Afterwards, after by the other end outgoing of fibre bundle 111, by showing
Lenticule 112 is focused on sample 2；Afterwards, according to the reversible principle of light path, the fluorescence that sample 2 goes out through excitation is through micro-
Lens 112 are coupled into the current time exciting light incident optical in fibre bundle 111, then through transmission after the 3rd object lens 110
To pipe mirror 109；Scanned lens 108, the object lens 106 of two-dimensional scanner 107 and second enter confocal pinhole 105 successively afterwards, altogether
Burnt pin hole 105 can hinder the fluorescence that other optical fiber in fluorescent light beam, in addition to current time exciting light incident optical are collected into
Gear falls；Afterwards, collimated light is formed through the first object lens 104 by the fluorescence that confocal pinhole 105 filters out, the collimated light is again by dichroic mirror
103 reflex to transmitting optical filter 113, excite light blocking to fall remnants by transmitting optical filter 113, filter out fluorescence；Afterwards, filter out
A fluorescence part transmitted by fluorescence beam splitter 114, focused on via the first condenser lens 115 on detector 116, by detecting
Device 116 obtains confocal fluorescent micro-image；It is radiated at after the transmitting filtering of optical filter 113 glimmering on fluorescence beam splitter 114
Light, another part are reflected by fluorescence beam splitter 114, and are focused on by the second condenser lens 117 on spectrometer 118, by spectrometer
118 obtain fluorescence spectrum.
In the utility model, in order to obtain the fluorescence confocal image of sample 2, two-dimensional scanner 107 often causes exciting light
Light beam deflect a step, by the 3rd object lens 110 focus on be coupled in fibre bundle 111, the optical fiber for importing exciting light also changes therewith,
The position for exciting luminous point of exciting light also corresponds to change in sample 2, to reach the purpose of scanning sample.And in fibre bundle 111 with
The fluorescence that root optical fiber of each position correspondence is collected forms confocal detection with confocal pinhole 15, therefore optical fiber collection is glimmering
Light can filter, and the fluorescence that other optical fiber in fibre bundle 111 are collected then is stopped by confocal pinhole 105.So, relative to tradition
The mode that confocal optical path is realized merely with fiber optic aperture in fibre bundle, the system introduce confocal pinhole 105 and realize really
Conjugate imaging, the interference fluorescence signal that other adjacent fibers in fibre bundle are collected can be filtered out, and tradition using optical fiber from
The mode in body aperture can not then realize the effect.Self solved in addition, confocal pinhole 105 is in scanning optical path, both realize exciting light
Point source effect, a thing detection of luminescence is realized again, compared to double-pore structure, it is simpler, performance is more stable, is advantageous to system production
Industry.
Two-dimensional scanner 107 in the utility model can be 2-D vibration mirror, acousto-optical device or other achievable grid scannings
Or the scanner of random scanning.
Dichroic mirror 103 in the utility model has to be all-trans and high saturating to exciting light and fluorescence to wide field imaging Laser
Characteristic.
Detector 116 in the utility model can be photomultiplier (Photomultiplier Tube, PMT) or its
Its single point detector；
Two-dimensional scanner 107, detector 116, spectrometer 118 in the utility model are all controlled by terminal 119, and by end
The storage of end 119 and display data.
Fluorescent confocal microscopy endoscopic imaging system provided by the utility model, including：Fluorescence excitation unit, image obtain single
Member, spectrum acquiring unit, fluorescence excitation unit, for exciting testing sample to obtain fluorescence, image acquisition unit, for obtaining
Confocal fluorescent micro-image, spectrum acquiring unit, for obtaining fluorescence spectrum.In fluorescent confocal provided by the utility model is micro-
It is on the basis of existing fluorescent confocal microscopy endoscopic imaging system to peep imaging system, and increase can obtain fluorescence spectrum information
The optics that spectrometer and light path are used cooperatively, to realize fluorescence spectrum with being shown while confocal fluorescence microscope images,
Solve existing fluorescent confocal microscopy endoscopic imaging system not having while be copolymerized burnt spectral analysis capabilities, when running into some things
The structure of body is similar, and light intensity detection can not be distinguished the problem of discrimination simultaneously to it, meanwhile, fluorescence provided by the utility model
Confocal microscopy endoscopic imaging system is advantageous to the commercial application of system.
In the above-described embodiments, the description to each embodiment all emphasizes particularly on different fields, and does not have the portion being described in detail in some embodiment
Point, it may refer to the associated description of other embodiments.
It is above the description to fluorescent confocal microscopy endoscopic imaging system provided by the utility model, for this area
Technical staff, according to the thought of the utility model embodiment, there will be changes in specific embodiments and applications,
To sum up, this specification content should not be construed as to limitation of the present utility model.
Claims (8)
- A kind of 1. fluorescent confocal microscopy endoscopic imaging system, it is characterised in that the fluorescent confocal microscopy endoscopic imaging system bag Include：Fluorescence excitation unit, image acquisition unit, spectrum acquiring unit；The fluorescence excitation unit, for exciting testing sample, to obtain fluorescence；Described image acquiring unit, for obtaining confocal fluorescent micro-image；The spectrum acquiring unit, for obtaining fluorescence spectrum.
- 2. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 1, it is characterised in thatThe fluorescence excitation unit, it is included in the LASER Light Source being sequentially placed in fluorescence excitation light path, exciter filter, double-colored It is mirror, the first object lens, confocal pinhole, the second object lens, two-dimensional scanner, scanning lens, Guan Jing, the 3rd object lens, fibre bundle, micro- Mirror.
- 3. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 2, it is characterised in thatDescribed image acquiring unit, be included in confocal fluorescent micro-image obtain light path on be sequentially placed the microlens, The fibre bundle, the 3rd object lens, the Guan Jing, the scanning lens, the two-dimensional scanner, second object lens, institute State confocal pinhole, first object lens, the dichroic mirror, transmitting optical filter, fluorescence beam splitter, the first condenser lens, detector.
- 4. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 3, it is characterised in thatThe spectrum acquiring unit, it is included in fluorescence spectrum and obtains the microlens, the optical fiber being sequentially placed in light path Beam, the 3rd object lens, the Guan Jing, the scanning lens, the two-dimensional scanner, second object lens, the confocal pin Hole, first object lens, the dichroic mirror, the transmitting optical filter, the fluorescence beam splitter, the second condenser lens, spectrometer.
- 5. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 4, it is characterised in thatThe LASER Light Source is used to produce exciting light；The exciter filter is placed in the light path for the exciting light that the LASER Light Source is sent；The dichroic mirror is placed on the exciting light in the light path after the exciter filter；First object lens are placed on the exciting light in the light path after dichroic mirror transmission；The confocal pinhole is placed on the exciting light in the light path after first object lens convergence；Second object lens are placed on the exciting light in the light path after the confocal pinhole；The two-dimensional scanner is placed on the exciting light in the light path after second object lens collimation；The scanning lens is placed on the exciting light in the light path after the two-dimensional scanner two-dimensional scan, and the two dimension is swept Retouch the front focal plane that device is placed on the scanning lens；The Guan Jing is placed on the exciting light in the light path after the scanning lens, the back focal plane of the scanning lens and institute The front focal plane for stating Guan Jing overlaps；3rd object lens are placed on the exciting light in the light path after pipe mirror collimation；The fibre bundle is placed on the exciting light in the light path after the 3rd object lens convergence, and the exciting light is through described the Three object lens are coupled into the fibre bundle；The microlens is placed on the exciting light in the light path after the fibre bundle；The transmitting optical filter is placed on fluorescence in the light path after dichroic mirror reflection, and the fluorescence is by the exciting light Obtained by exciting the testing sample；The fluorescence beam splitter is placed on the fluorescence through in the transmitting filtered light path of optical filter；First condenser lens is placed on the fluorescence in the light path after fluorescence beam splitter transmission；The detector is placed on the fluorescence after first condenser lens focusing in light path, and the detector is placed on institute State the back focal plane of the first condenser lens；Second condenser lens is placed on the fluorescence in the light path after fluorescence beam splitter reflection；The spectrometer is placed on the fluorescence after second condenser lens focusing in light path, and the spectrometer is placed on institute State the back focal plane of the second condenser lens.
- 6. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 5, it is characterised in that the system also includes：Terminal；The terminal is electrically connected with the two-dimensional scanner, the detector, the spectrometer respectively.
- 7. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 6, it is characterised in that the aperture of the confocal pinhole It is sized to adjust.
- 8. fluorescent confocal microscopy endoscopic imaging system as claimed in claim 7, it is characterised in that the fibre bundle includes more Root optical fiber.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107957401A (en) * | 2017-12-18 | 2018-04-24 | 中国科学院西安光学精密机械研究所 | A kind of EO-1 hyperion microscopic imaging apparatus available for insertion type diagnosing tumor |
| CN110464309A (en) * | 2019-08-27 | 2019-11-19 | 深圳大学 | A kind of fluorescent endoscopic imgaing system across scale |
| CN111711750A (en) * | 2020-06-05 | 2020-09-25 | 腾讯科技（深圳）有限公司 | Image processing method, device, equipment and medium based on artificial intelligence |
-
2017
- 2017-04-10 CN CN201720371701.5U patent/CN206627441U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107957401A (en) * | 2017-12-18 | 2018-04-24 | 中国科学院西安光学精密机械研究所 | A kind of EO-1 hyperion microscopic imaging apparatus available for insertion type diagnosing tumor |
| CN110464309A (en) * | 2019-08-27 | 2019-11-19 | 深圳大学 | A kind of fluorescent endoscopic imgaing system across scale |
| CN111711750A (en) * | 2020-06-05 | 2020-09-25 | 腾讯科技（深圳）有限公司 | Image processing method, device, equipment and medium based on artificial intelligence |
| CN111711750B (en) * | 2020-06-05 | 2023-11-07 | 腾讯科技（深圳）有限公司 | Image processing method, device, equipment and medium based on artificial intelligence |
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