CN206621452U - The preparation facilities of lumen organization's construct - Google Patents
The preparation facilities of lumen organization's construct Download PDFInfo
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- CN206621452U CN206621452U CN201621053494.0U CN201621053494U CN206621452U CN 206621452 U CN206621452 U CN 206621452U CN 201621053494 U CN201621053494 U CN 201621053494U CN 206621452 U CN206621452 U CN 206621452U
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Abstract
It the utility model is related to the preparation facilities of lumen organization's construct.Preparation facilities includes：First supporting part, for carrying the outer wall (1) of lumen organization's construct；Second supporting part (4), for carrying the biological construct (2) of lumen organization's construct；And drive mechanism, for making first supporting part and second supporting part (4) to relatively move, so that the biological construct (2) is attached on the inner peripheral surface of the outer wall (1).Using the technical scheme of the application, by being arranged the biological construct (2) comprising bioactive substance in the outer wall (1) of lumen organization's construct, to simulate the biological lumen tissue of organism, therefore the problem of artificial lumen organization can not be adapted to human body environment present in prior art can be improved.
Description
Technical field
Bioartificial tissue field is the utility model is related to, in particular to a kind of preparation of lumen organization's construct
Device.
Background technology
Blood vessel grafting can be used for the blood vessel of narrow, inaccessible, expansion, damage or deformity is rebuild or repaired.It is common
Blood vessel graft source be autologous patient artery or vein, still, autologous patient vascular supply deficiency situation
Under (such as patient with vascular disease or previously had been carried out blood vessel grafting), it is necessary to be made using artificial blood vessel or heterologous blood vessel
For substitute.
Existing artificial blood vessel is made up of polymer fiber (such as nylon, terylene), silk or expanded PTFE.
When carrying out vasotransplantation, blood vessel of problems is rebuild using complete artificial blood vessel, or also utilize sheet
Or the artificial blood vessel such as bulk.Problematic blood vessel is repaired.Although using this artificial blood vessel to lesion or impaired blood
Pipe, which is replaced or repaired, has clinically obtained huge effect, but it still faces insoluble problem, when being included in long
Between be implanted into after thrombus again occur and tube chamber ISR appearance.The basic reason of these problems is caused to be, it is this artificial
The inwall of blood vessel lacks complete endothelial layer.
Have lot of experiments at present to attempt to solve the above problems, correlation technique includes：Adhere in artificial blood vessel's inwall
Inducible factor, attract adhesion, differentiation and the growth of the stem cell (such as endothelial progenitor cells) in blood；Applied in artificial blood vessel's inwall
Biomaterial is smeared, promotes to be planted in the differentiation of stem cell or the adhesion of adult cell and growth thereon.But to being at present
Only, these technologies can not be realized all the time forms complete endothelial layer in artificial blood vessel's inwall, is attached to artificial blood vessel's inwall
Cell it is easy to fall off, it is difficult to normal differentiation and survival, do not possess preferably biological function, may influence vasotransplantation into
Using effect after power and transplanting, thus be difficult to meet clinical demand.
Utility model content
The preparation facilities of the utility model lumen organization construct, to improve artificial lumen organization present in prior art
Do not possess preferably biological function.
According to the one side of the utility model embodiment, the utility model provides a kind of lumen organization construct
Preparation facilities, preparation facilities includes：
First supporting part, for carrying the outer wall of lumen organization's construct；
Second supporting part, for carrying the biological construct of lumen organization's construct；And
Drive mechanism, for enabling the first supporting part and the second supporting part to relatively move, so that biological construct attaches
On the inner peripheral surface of outer wall.
Alternatively, in addition to for being tensioned or expanding the pipe expander of outer wall.
Alternatively, pipe expander includes cavity and the air entry being arranged on cavity, and the first supporting part includes tube-like piece, manages
Shape part is set in the outside of outer wall, and aperture is provided with tube-like piece, and tube-like piece is arranged in cavity.
Alternatively, there is gap between tube-like piece and the inner peripheral surface of cavity, gap along tube-like piece circumferentially.
Alternatively, cavity has opening end, and tube-like piece is inserted in cavity by opening end, the remote opening end of tube-like piece
One end is closing.
Alternatively, cavity has the bottom relative with opening end, and bottom setting is fluted, the remote opening end of tube-like piece
One end plugs in a groove.
Alternatively, one end of the close opening end of tube-like piece is provided with boss, and boss is by the periphery of tube-like piece towards cavity
Inner peripheral surface extension, boss circumferentially, seal is provided between boss and the inner peripheral surface of cavity along tube-like piece.
Alternatively, holding tank is provided with the end face towards cavity of boss, seal is arranged in holding tank.
Alternatively, pipe expander also includes the fluid passage extended along the outer peripheral face of outer wall.
Alternatively, fluid passage extends spirally along the axially extending of outer wall, or fluid passage along the circumference of outer wall.
Alternatively, in addition to for the spray gun for outer wall and/or biological construct spraying adhesive.
Alternatively, the movement that spray gun and outer wall can be relative, the spout of spray gun can be towards the inner surface of outer wall；Or
Spray gun and biological construct can be relative movement, the spout of spray gun can be towards the outer surface of biological construct.
Using the technical scheme of the application, by being arranged in the outer wall of lumen organization's construct comprising bioactive substance
Biological construct, to simulate the biological lumen tissue of organism, artificial lumen organization present in prior art can be improved
Do not possess preferably biological function.
It is of the present utility model other by referring to the drawings to the detailed description of exemplary embodiment of the present utility model
Feature and its advantage will be made apparent from.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the utility model, for those of ordinary skill in the art, before creative labor is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 shows the stereochemical structure signal of the preparation facilities of lumen organization's construct of embodiment of the present utility model
Figure；
Fig. 2 shows the sectional view of the preparation facilities of lumen organization's construct of embodiment of the present utility model；
Fig. 3 shows the explosive view of the preparation facilities of lumen organization's construct of embodiment of the present utility model；And
Fig. 4 shows that the structure of the tube-like piece of the preparation facilities of lumen organization's construct of embodiment of the present utility model is shown
It is intended to.
In figure：1st, outer wall；2nd, biological construct；3rd, tube-like piece；4th, the second supporting part；5th, cavity；6th, air intake duct；7th, it is convex
Platform；8th, seal；9th, aperture；10th, holding tank；11st, air entry.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.The description only actually at least one exemplary embodiment is illustrative to be never used as to this practicality below
New and its application or any restrictions used.Based on the embodiment in the utility model, those of ordinary skill in the art are not having
There is the every other embodiment made and obtained under the premise of creative work, belong to the scope of the utility model protection.
Term " tube chamber " used in this application refers to be shaped as tubulose, has the organ of hollow cavity, such as circulates
Tube chamber, digestive tract cavity, breathing tube chamber, uropoiesis tube chamber or reproduction tube chamber, such as blood vessel, oesophagus, tracheae, stomach, bile duct, enteron aisle (bag
Include small intestine and large intestine, such as duodenum, jejunum, ileum, caecum (including appendix), the colon ascendens, flexura coil dextra, transverse colon, knot
The left song of intestines, colon descendens, sigmoid colon, rectum), fallopian tubal, vas deferens, ureter, bladder or lymphatic vessel).
Term " tissue " used in this application refers to be made up of homomorphosis or similar, function identical cell mass
Cell aggregate, and the material (being referred to as cytoplasm, such as matrix, fiber etc.) generally also comprising acellular form.Tissue
It may include one or more cells.
Term " micro-capsule " used in this application refers to, micro-structural containing cell and biocompatible materials (for example,
Micron order is to millimetre-sized structure), wherein, cell is wrapped in the biocompatible materials.Micro-capsule of the present utility model
(such as 4-37 DEG C, such as pH is between 6-8, such as under the hydrodynamic shear of physiological environment) has stable under physiological environment
Structure.Preferably, micro-capsule has the mechanical strength that will not cause micro-capsule broken in absorption or extruding.
Term " biological construct " used in this application refers to the object built using micro-capsule of the present utility model, its
Can have two dimension or three-dimensional structure, can be used for preparing artificial lumen organization's construct.
Term " biocompatible materials " used in this application refers to such material, its (and its catabolite)
It is avirulent for cell, and in implantation host (such as human body) afterwards and host compatibility, will not causes significant or tight
The side effect of weight, for example, toxic action will not be caused to host (such as tissue), will not cause the immunological rejection of host anti-
Should, allergic reaction or inflammatory reaction etc..
Term " Biodegradable material " used in this application refers to such material, and it can be by cell or biology
Body is degraded and absorbed, and its catabolite is biocompatibility.Such material can be natural origin (such as from
Animals and plants) or it is artificial synthesized.
Fig. 1 shows the dimensional structure diagram of the preparation facilities of lumen organization's construct of the present embodiment；Fig. 2 is shown
The sectional view of the preparation facilities of lumen organization's construct of the present embodiment；Fig. 3 shows lumen organization's construct of the present embodiment
Preparation facilities explosive view.
With reference to shown in Fig. 1 to 3, the preparation facilities of lumen organization's construct of the present embodiment includes being used to carry lumen organization
Second supporting part 4 of the first supporting part of the outer wall 1 of construct and the biological construct 2 for carrying lumen organization's construct,
Second supporting part 4 can move relative to the first supporting part, so that the second supporting part 4 carries biological construct 2 and is attached to outer wall 1
Inner surface.
Can also be that the first supporting part can move relative to the second supporting part 4, so that the second supporting part 4 carries biology
Construct 2 is attached to the inner surface of outer wall 1.
Can also be that the first supporting part can move relative to the second supporting part 4, and the second supporting part 4 can be relative to
First supporting part moves, so that the second supporting part 4 carries the inner surface that biological construct 2 is attached to outer wall 1.
In the present embodiment, by being pasted with the biological structure comprising bioactive substance in the outer wall 1 of lumen organization's construct
Body 2 is built, to simulate the biological lumen tissue of organism, therefore can improve present in prior art that artificial lumen organization can not
The problem of being adapted to the environment of human body so that lumen organization's construct has good biological function.
In the present embodiment, outer wall 1 is the solid support for supporting biological construct 2, and biological construct 2 is attached to outer
On the inner surface of wall 1, biological construct 2 is used for the lumen organization for simulating organism, to improve the artificial tube chamber group of prior art
The problem of knitting the environment that can not be adapted to body.
Wherein, the outer wall 1 of lumen organization's construct can be artificial material.Alternatively, artificial material be nylon, terylene and
One kind in polytetrafluoroethylene.The material of outer wall 1 can also be natural material, such as the lumen organization of animal.
The material preferred biocompatible material of outer wall 1, outer wall 1 and its inside host catabolite for cell
Be it is avirulent, and outer wall 1 after host is implanted into host compatibility, significant or serious side effect will not be caused.
Outer wall 1 is optionally Biodegradable material.Biodegradable material can be natural degradable biomaterial, example
As collagen, gelatin and modified gelatin (such as the cross-linking modified gelatin of dialdehyde starch DAS), chitosan, poly butyric ester (PHB),
Chitin, alginate (such as sodium alginate) and modified alginates (such as oxidized sodium alginate), starch-based bio material
(such as starch and poly amylose), cellulose (such as bacteria cellulose), fibroin, and its any combination.
Biodegradable material is alternatively synthesized degradable material, for example, aliphatic polyester, poly- hydroxyl valerate (PHV),
Poly butyric ester (PHB), poly butylene succinate (PBS)), polyglycolic acid (PGA), polylactic-co-glycolic acid
(PLGA), poe (POE), degradability polyurethane (such as starch conversion polyurethane), polyvinyl alcohol, poly- to dioxocyclohex
Ketone, poly-p-dioxanone, poly- dioxane ketone, polytetramethylene carbonate diol, polyphosphazene and its any combinations.
Alternatively, outer wall 1 is biological non-degradable material, such as nylon, terylene, polypropylene, polyethylene, polytetrafluoroethyl-ne
Alkene, silicon rubber, fluorosioloxane rubber, natural rubber, polyacrylate, aromatic polyester (such as polyethylene terephthalate
(PET)), nondegradation polyurethane, polyether-ether-ketone, polyacrylonitrile, polysiloxanes, polyformaldehyde, polyvinyl chloride and its any group
Close.
Outer wall 1 as the solid support of biological construct 2 is impregnated by mould, electrostatic spinning, extrusion forging, 3D are beaten
Print or spraying are made.
In certain preferred aspects, the method that solid support is impregnated by mould obtains.Preferably, the mould
Tool infusion process comprises the steps of：
(1) by for the material (such as Biodegradable material) for preparing solid support be dissolved in suitable solvent (such as
Organic solvent, such as chloroform, tetrahydrofuran or DMA) in, it is configured to prepare solution；
(2) mould is immersed in the preparation solution, takes out mould, the solvent on mould is volatilized；
(3) repeat step (2) repeatedly, obtains solid support；
Optionally, methods described is further comprising the steps of：
Solid support is dried, shear and/or sterilized, to form the outer wall 1 of tubulose biological tissue construct.
The bioactive substance of biological construct 2 includes micro-capsule, and micro-capsule includes the cell of animal and is wrapped in extracellular
Biocompatible materials.Multiple micro-capsules arrange to form the biological construct of sheet, strip, bulk or tubulose by ranks.
The species for the cell that micro-capsule includes can be selected according to being actually needed, and be not particularly limited.Preferably, it is micro-
Endothelial cell (such as vascular endothelial cell), smooth muscle cell (such as vascular smooth muscle cells) and/or undifferentiated are included in capsule
Cell.
Preferably, the cell in micro-capsule is undifferentiated cell, such as stem cell (such as fat mesenchymal stem cell, bone
Bone marrow-drived mesenchymal stem, induced multi-potent stem cell and embryonic stem cell).
Preferably, undifferentiated cell can be divided into endothelial cell and/or smooth muscle cell.
Preferably, undifferentiated cell be selected from stem cell (such as fat mesenchymal stem cell, mesenchymal stem cells MSCs,
Induced multi-potent stem cell and embryonic stem cell) and progenitor cells (such as endothelial progenitor cells) in one or more.
In the present embodiment, the source for the cell that micro-capsule includes can be selected according to being actually needed, without especially being limited
System.Preferably, the cell that micro-capsule includes is obtained from animal, such as mammal, such as people, ape, monkey, gorilla, ox, pig, dog, silk floss
Sheep and goat.
Preferably, the cell derived is in selected from following tissues：Connective tissue is (for example, loose connective tissue, fine and close knot
Form tissue, elastic fibrous tissue, reticular connective tissue and adipose tissue), musculature (for example, skeletal muscle, smooth muscle and cardiac muscle), secrete
Germinal tissue, gastrointestinal tissue, lung tissue, bone tissue, nerve fiber and epithelial tissue are urinated (for example, on simple epithelium and cladding
Skin), the tissue of endoderm origin, the tissue of the tissue of mesoderma origin and ectodermal origin.
The quantity for the cell that micro-capsule includes can be selected according to being actually needed, and be not particularly limited.For example, this reality
1-10 can be included independently of one another with the stratum nucleare of new micro-capsule6Individual cell.
In the present embodiment, outer wall 1 and biological construct 2 are in a tubular form.Above-mentioned tubulose can be it is circumferentially closed, can also
It is that there is opening in circumference.The biological construct 2 of tubulose is set in outer wall 1, and is attached on the inner surface of outer wall 1.
Preferably, tubulose biological construct is prepared to carry out by the method comprised the steps of：
(1) one or more micro-capsules are provided, its all or part of surface attachment has the first component；Preferably, described
One component is contained in the first reagent；
(2) the second reagent containing the second component is coated with the predeterminable area of interim support surface, wherein, when described
When first component contacts with the second component, adhesion effect can be produced, realizes adhesive effect；Interim holder is tubulose or column
(such as side wall be not open it is round tubular, sidewall opening it is round tubular, it is cylindric or along part-circular periphery set column) thing,
Predeterminable area is located at the curved surface of interim holder；Optionally, before the second reagent is coated with, backing material is coated on interim branch
On the predeterminable area for holding thing surface；
(3) micro-capsule that all or part of surface attachment in step (1) has the first component is positioned over and is coated with second
The predeterminable area of reagent, the first component on micro-capsule surface is contacted with the second component on predeterminable area, produce adhesion effect,
So as to which by micro-capsule assembling (bonding), into the first Rotating fields, the first Rotating fields are tubular structure；
Optionally, the method for preparing tubulose biological construct is further comprising the steps of：
(4) it is being coated with the second reagent on the first Rotating fields caused by step (3)；
(5) micro-capsule that all or part of surface attachment in step (1) has the first component is positioned over previous step production
On the first raw Rotating fields, the first component on the micro-capsule surface is contacted with the second component on the first Rotating fields, produce
Adhesion effect, so as to which on the first Rotating fields, micro-capsule is being assembled into (bonding) into another Rotating fields caused by previous step；
(6) optionally, repeat step (4) and (5) are one or many；For example, at least 1 time, at least 2 times, at least 3 times, at least
4 times, at least 5 times, at least 10 times, at least 15 times, at least 20 times, at least 30 times, at least 40 times, at least 50 times, at least 100 times,
At least 200 times, at least 500 times, or more time；So as to obtain tubulose biological construct.
Optionally, preparing the method for tubulose biological construct also includes：By the round tubular biological construct of sidewall opening
Bonded, obtain the round tubular biological construct that side wall is not open.
Optionally, preparing the method for tubulose biological construct also includes：By tubulose biological construct 2 and interim holder point
From.
Preferably, interim holder is the print platform with curved surface, such as the swingle of biometric print machine.
Preferably, backing material is Thermo-sensitive material, such as gelatin, poly-N-isopropyl acrylamide, poly-N-isopropyl third
Acrylamide-polyethyleneglycol block copolymer, ethylene glycol copolymer (such as Kollicoat IR), poly- hydroxyl second
Base acrylate, agarose, Matrigel, chitosan/sodium glycero-phosphate system or Pluronic F127.
Preferably, interim holder is Thermo-sensitive material (such as gelatin, poly-N-isopropyl acrylamide, poly-N-isopropyl
Acrylamide-polyethyleneglycol block copolymer, ethylene glycol copolymer, polyhydroxyethyl acrylate, agarose, Matrigel,
Chitosan/sodium glycero-phosphate system or Pluronic F127) made of cylinder or circular tube shaped thing.
In certain preferred aspects, interim holder is cylinder.
In certain preferred aspects, interim holder is cylinder, and the predeterminable area is cylinder
Whole side, so as to which the first Rotating fields that step (3) obtains are the round tubular structure that side wall is not open.
In certain preferred aspects, interim holder is cylinder, cylinder of the predeterminable area in expansion
It is a rectangle on the side of shape thing, and side axially through cylinder of the predeterminable area along cylinder, so as to
The first Rotating fields that step (3) obtains are the round tubular structure that side wall is not open.
In certain preferred aspects, the interim holder is cylinder, and the predeterminable area is in expansion
It is a rectangle on the side of cylinder, and radial direction of the predeterminable area along cylinder penetrates column side, so as to
The first Rotating fields that step (3) obtains are the round tubular structure that side wall is not open.
In certain preferred aspects, the interim holder is cylinder, and the predeterminable area is in expansion
It is a rectangle on the side of cylinder, and not radially or axially through cylinder side, is obtained so as to step (3)
First Rotating fields are the round tubular structure of sidewall opening.
In the present embodiment, the second supporting part 4 includes the body of rod, and the body of rod is as above-mentioned interim holder.In the second supporting part 4
The body of rod on print biological construct 2 after, the body of rod carry biological construct 2 enter outer wall 1 inner chamber in so that biology build
Body 2 is attached on the inner surface of outer wall.
Preferably, in step (3), step (2) is positioned in the micro-capsule that all or part of surface attachment is had into the first component
Be coated with after the predeterminable area of the second reagent, stand 0.1-60s；(such as 0.1-1s, 1-5s, 5-10s, 10-15s, 15-
20s, 20-25s, 25-30s, 30-35s, 35-40s, 40-45s, 45-50s, 50-55s, or 55-60s).The standing step is favourable
Fully contact, and interact with the second component on predeterminable area in the first component on the micro-capsule surface, so as to
The micro-capsule is assembled into (bonding) into the first Rotating fields.
Preferably, the method for preparing tubulose biological construct is implemented by biometric print method.
Preferably, biometric print method is carried out using printer (such as 3D biometric prints machine)；Or using automation or
Non-automated mechanical process carries out biometric print method；Or by using placing by hand or manual deposition method (such as uses shifting
Liquid device) carry out biometric print method.
In the method for the present utility model for preparing artificial organ precursor, it is preferable that first component and/or second group
It is divided into biocompatible materials, and/or to be Biodegradable material from the material of biology.
In certain preferred aspects, first component contacts with the second component and caused adhesion effect can use
It is bonded together in by two micro-capsules, forms construct；And the stretch modulus of the construct thus obtained is not less than 10Pa,
Such as not less than 20Pa, not less than 30Pa, not less than 40Pa, not less than 50Pa, not less than 60Pa, not less than 70Pa, it is not less than
80Pa, not less than 90Pa, not less than 100Pa, not less than 200Pa, not less than 300Pa, not less than 400Pa, not less than 500Pa,
Not less than 600Pa, not less than 700Pa, not less than 800Pa, not less than 900Pa, not less than 1000Pa.
Preferably, first component and the second component are to be selected from following combination：
(1) fibrinogen and fibrin ferment；
(2) alginate (such as sodium alginate) or oxidation alginate (such as sodium alginate of oxidation) and contain
Ca2+, Mg2+, Ba2+, Sr2+ or Fe3+ material (such as solution containing Ca2+, Mg2+, Ba2+, Sr2+ or Fe3+ or half
Solid (such as gel))；
(3) molecule containing maleimide base group (such as polyethylene glycol (MAL-PEG) containing maleimide base group)
With the molecule (such as polyethylene glycol (PEG-SH) containing free sulfhydryl group) containing free sulfhydryl group；
(4) material containing anion (such as solution or semisolid (such as gel) containing anion) and alpha-cyano third
Olefin(e) acid ester (such as Mecrilate, α-cyanoacrylate, isobutyl alpha-cyanoacrylate, alpha-cyanoacrylate
Dissident's ester, α-n-octylcyanoacrylate)；
(5) fibrinogen and a-cyanoacrylate (such as Mecrilate, α-cyanoacrylate,
Isobutyl alpha-cyanoacrylate, alpha-cyanoacrylate dissident's ester, α-n-octylcyanoacrylate)；
(6) seralbumin (for example, bovine serum albumin(BSA)) and glutaraldehyde；
(7) molecule of acid esters containing urethane groups (- NHCOO-) or isocyanate groups (- NCO) is (such as containing carbamic acid
The polyethylene glycol of ester group or the polyethylene glycol containing isocyanate groups) and molecule (such as carboxylic poly- second containing active hydrogen
Glycol)；
(8) gelatin-resorcinol and glutaraldehyde；
(9) carbodiimides cross-linked gelatin and L-glutamic acid (PLGA)；With
(10) aminated gelatin and aldehyde radical polysaccharide.
Certainly, biological construct 2 can also be sheet, strip or bulk.The biological construct patch of sheet, strip or bulk
It is attached on the inner surface of outer wall 1.
In the present embodiment, lumen organization's construct is blood vessel.Certain those skilled in the art have the ability the present embodiment
The preparation facilities of lumen organization's construct is used to prepare any tubulose biological tissue.
By setting the biological construct 2 for including bioactive substance in the outer wall 1 of lumen organization's construct so that blood
The endothelialization of biological construct 2 of pipe, advantageously reduce thrombosis, improve the long-term patency rate of blood vessel.Be advantageous to improve existing
Easily there is thromboembolism, doped calcium, narrow or infection after implantation within a patient in artificial blood vessel's substitute present in technology
The problem of.
In the present embodiment, outer wall 1 and biological construct 2 are in a tubular form.Alternatively, the outer wall 1 of lumen organization's construct is
Flexible material, therefore the internal diameter of outer wall 1 can expand in order to which biological construct 2 is set in outer wall 1.
In order that the biological construct 2 of lumen organization's construct can be smoothly set in outer wall 1 to be attached to outer wall 1
Inner surface on, the preparation facilities of lumen organization's construct also includes being used for the pipe expander for being tensioned or expanding outer wall 1.
Pipe expander includes cavity 5 and the air entry 11 being arranged on cavity 5, and the first supporting part includes tube-like piece 3, outer wall
1 is set in tube-like piece 3, and aperture 9 is provided with tube-like piece 3, and tube-like piece 3 is arranged in cavity 5.
Second supporting part 4 includes the body of rod, and the biological construct 2 of lumen organization's construct is set on the body of rod.
Air entry 11 on cavity 5 is connected with air intake duct 6, and air intake duct 6 is connected with suction device.Suction device aspirates air
When, the pressure in aperture 9 diminishes, and the inside pressure of outer wall 1 is more than the pressure in aperture 9, therefore outer wall 1 is in its inside pressure
Drawn close under effect to the inner peripheral surface of tube-like piece 3, to be tensioned or expand outer wall 1.
There is gap between the inner peripheral surface of tube-like piece 3 and cavity 5, gap along tube-like piece 3 circumferentially.Tube-like piece 3
Spreading all on perisporium has aperture 9, and aperture 9 can communicate with air entry 11.
By aspirating the outer wall 1 of the air lumen organization construct between cavity 5 and tube-like piece 3 to tube-like piece 3
Inner peripheral surface is drawn close, to be tensioned or expand outer wall 1 so that biological construct 2 is easy to be set in outer wall 1.
After biological construct 2 is set in outer wall 1, stops the air of swabbing pressure intracavitary or pressure is provided into pressure chamber
Power, so that biological construct 2 fits with outer wall 1.
With reference to shown in Fig. 3 and Fig. 4, in the present embodiment, tube-like piece 3 and the split settings of cavity 5.Cavity 5 has opening end, pipe
Shape part 3 is inserted in cavity 5 by opening end, and one end of the remote opening end of tube-like piece 3 is closing.
Cavity 5 has the bottom relative with opening end, and bottom sets fluted, one end of the remote opening end of tube-like piece 3
Plug in a groove, to fix one end of the remote opening end of tube-like piece 3, so that tube-like piece 3 and cavity 5 are coaxial.
Can also preferably, one end of the remote opening end of tube-like piece 3 is unlimited.The remote opening end of tube-like piece 3
One end is plugged in the groove of the bottom of cavity 5, and potted component is set between the perisporium of groove and the side face of tube-like piece 3, with envelope
Close one end of the remote opening end of tube-like piece 3.
One end of the close opening end of tube-like piece 3 is provided with boss 7, and boss 7 is by the periphery of tube-like piece 3 towards cavity 5
Inner peripheral surface extends, and seal 8 is provided between boss 7 and the inner peripheral surface of cavity.The inner peripheral surface of boss 7 and cavity 5 coordinates can be with
Positioned tubular part 3.Further, seal 8 is used for the gap between sealed tubular part 3 and cavity 5, when aspirating air to improve
The power being applied on the outer wall 1 of lumen organization's construct.
In the present embodiment, boss 7 towards being provided with holding tank 10 on the end face of cavity 5,
Seal 8 is arranged in holding tank 10.Holding tank 10 can play a part of positioning seals 8, in order to manage
Shape part 3 is inserted in cavity 5.
Can also preferably, pipe expander includes the fluid passage extended along the outer peripheral face of outer wall 1, to be imitated using Bernoulli Jacob
The outside pressure of outer wall 1 should be reduced.According to the principle of Bernoulli effect：When fluid velocity is accelerated, boundary that object contacts with fluid
Pressure on face can reduce.By setting the increased fluid of flow velocity in the fluid passage in the outside of outer wall 1 so that outer wall 1
Outside pressure is less than the inside pressure of outer wall 1, so as to which outer wall 1 to be adsorbed to the inner peripheral surface of tube-like piece 3.
Fluid passage can be along the axially extending of outer wall 1, or circumference of the fluid passage along outer wall 1 extends spirally.
Specifically, outer wall 1 is set in tubular body, and body is arranged in the cavity consistent with its bearing of trend, on body
Strip through-hole is provided with, strip through-hole can spirally extend along the axially extending of body or along the circumference of body, to form stream
Body passage.During the flow velocity increase of the fluid in fluid passage, fluid is less than outer wall 1 with the pressure at the contact surface of outer wall 1
Inside pressure, so that outer wall 1 is drawn close to the inner peripheral surface of body, therefore outer wall can be tensioned or be expanded.
Preferably, pipe expander includes being used to fix the fixing device of the first end of outer wall 1 and for the first of outer wall 1
The blower fan of air-flow is provided in the port at end.The air-flow that blower fan is provided is flowed from the first end of outer wall 1 to the second end, and to outer wall
1 circumference is disperseed so that the inside pressure of outer wall 1 is more than the outside pressure of outer wall 1, so as to be tensioned or expand outer wall 1
Internal diameter.
Biological construct 2 can be entered in the inner chamber of outer wall 1 by the second end of outer wall 1.Can also be that outer wall 1 is to biology
Construct 2 is moved to be set on biological construct 2.
The preparation facilities of lumen organization's construct of the present embodiment also includes being used to spray for outer wall 1 and/or biological construct 2
Apply the spray gun of binding agent.
Alternatively, spray gun can extend into the inner chamber of outer wall 1, and spray gun has the spout of the inner surface towards outer wall 1.Enter one
Step ground, spray gun can along outer wall 1 axial movement and/or can be rotated in the inner chamber of outer wall 1 so that spray gun can be outer wall
1 whole inner peripheral surface spraying adhesive.
Alternatively, spray gun can have the appearance towards biological construct 2 along the axial movement of biological construct 2, spray gun
The spout in face.Further, spray gun can be rotated around biological construct 2 so that spray gun can be the outer of whole biological construct 2
Side face spraying adhesive.
According to the another aspect of the application, the present embodiment additionally provides a kind of preparation method of lumen organization's construct, system
Preparation Method includes：
First, there is provided the outer wall 1 of lumen organization's construct and the biological construct 2 comprising bioactive substance；
Secondly, application of adhesive on one in the inner surface of the outer surface of biological construct 2 and outer wall 1, another
The curing agent for solidifying adhesive is coated on individual.On one in the inner surface of the outer surface of biological construct 2 or outer wall 1
When being attached with the material for solidifying binding agent, without in spraying curing agent.For example, when binding agent is biogum, in biology
When on the outer surface of construct 2 or the inner surface of outer wall 1 comprising the anion as the curing agent of biogum, without in outer wall
1 or biological construct 2 spray curing agent.
Then, the biological construct 2 comprising bioactive substance is attached to the inner surface of outer wall 1, to form tube chamber group
Knit construct.
Wherein, the outer wall 1 of lumen organization's construct is artificial material.Artificial material can be nylon, terylene and polytetrafluoro
One kind during second is dilute.The material of outer wall 1 can also be natural material, such as the lumen organization of animal.
In the present embodiment, outer wall 1 and biological construct 2 are in a tubular form.Above-mentioned tubulose can be it is circumferentially closed, can also
It is that there is opening in circumference.Biological construct 2 is set in outer wall 1, and is attached on the inner surface of outer wall 1.
Biological construct 2 can also be sheet, strip or bulk.The biological construct of sheet, strip or bulk is attached to
On the inner surface of outer wall 1.
The step that biological construct 2 comprising bioactive substance is attached in outer wall 1 is included：It is tensioned or expands first
Outer wall 1, in order to which biological construct 2 is set in outer wall 1；Then outer wall 1 and biological construct 2 are along tubulose biological construct
It is axially opposingly mobile, biological construct 2 is attached on outer wall 1.
Tensioning or expansion outer wall 1 include:The outside pressure of outer wall 1 is set to be less than the inside pressure of outer wall 1, outer wall 1 is in inner side
It is tensioned or expands in the presence of pressure.
Allow the outside pressure of outer wall 1 to be less than the method for inside pressure of outer wall 1 to be：First on the outside of outer wall 1
Form pressure chamber；Then the air of swabbing pressure intracavitary so that the outside pressure of outer wall 1 is less than the inside pressure of outer wall 1.
Outer wall 1 is tensioned or expanded in the presence of inside pressure in favor of biological construct 2 is set in outer wall 1, will
After biological construct 2 is set on outer wall 1, stops the air of swabbing pressure intracavitary or pressure is provided into pressure chamber, so that raw
Thing construct 2 is attached on the inner surface of outer wall 1.
Pressure chamber is formed on the outside of outer wall 1 to be included：Tube-like piece 3 with aperture 9 is set on outer wall 1；Will pipe
Shape part 3 is placed in cavity 5, to form pressure chamber between tube-like piece 3 and cavity 5.
Also it may be preferred that making the outside pressure of outer wall 1 include less than the inside pressure of outer wall 1：The shape on the inner side of outer wall 1
Into pressure chamber, then increase the pressure in pressure chamber so that the outside pressure of outer wall 1 is less than the inside pressure of outer wall 1.
For example, the first end of fixed outer wall 1, and provide air-flow in the port of the first end to outer wall 1.Air-flow is by outer wall 1
First end flowed to the second end, and disperse to the circumference of outer wall 1 so that the inside pressure of outer wall 1 is more than the outer side pressure of outer wall 1
Power, so as to be tensioned or expand the internal diameter of outer wall 1.
The outside pressure of outer wall 1 preferably, can also be made include less than the inside pressure of outer wall 1：Set along the outer of outer wall 1
The fluid of side flowing, to reduce the outside pressure of outer wall 1 using Bernoulli effect.
Alternatively, fluid flows along the axial direction of outer wall 1；Or, fluid spirally flows along the circumference of outer wall 1.
For example, outer wall 1 is set in tubular body, body is arranged in the cavity consistent with its bearing of trend, set on body
Strip through-hole is equipped with, strip through-hole can spirally extend along the axially extending of body or along the circumference of body, to form fluid
Passage.During the flow velocity increase of the fluid in fluid passage, fluid is less than the interior of outer wall 1 with the pressure at the contact surface of outer wall 1
Lateral pressure, so that outer wall 1 is drawn close to the inner peripheral surface of body, therefore outer wall can be tensioned or be expanded.
It note that " step 1 " and " step 2 " herein is merely representative of step 1 before step 2, between the two can be with
There is no other steps to arrange other steps.
According to the another aspect of the application, the present embodiment additionally provides a kind of lumen organization's construct, lumen organization's structure
Body includes outer wall 1 and the biological construct 2 comprising bioactive substance, biological construct 2 are attached on the inner surface of outer wall 1.
Alternatively, lumen organization's construct passes through above-mentioned preparation side by the preparation facilities of above-mentioned lumen organization's construct
Method is fabricated.By being pasted with the biological construct 2 of the biological tissue comprising activity in the outer wall 1 of lumen organization's construct,
To simulate the biological lumen tissue of organism, therefore it artificial lumen organization present in prior art can be improved can not be adapted to
The problem of environment of human body so that the biology performance of lumen organization's construct is adapted with the biology performance of host.
Outer wall 1 is biocompatible materials.Outer wall 1 and its catabolite inside host are non-toxic for cell
, and will not cause significant or serious side effect with host compatibility after host is implanted into.
Alternatively, outer wall 1 is Biodegradable material.Outer wall 1 can be degraded and absorbed by the cell of host or organism,
And its catabolite is biocompatibility.
Biodegradable material can be natural degradable biomaterial, such as collagen, gelatin and modified gelatin are (such as double
The cross-linking modified gelatin of starch aldehyde DAS), chitosan, poly butyric ester (PHB), chitin, alginate (such as sodium alginate)
And modified alginates (such as oxidized sodium alginate), starch-based bio material (such as starch and poly amylose), cellulose
(such as carboxymethyl cellulose, oxidized regenerated cellulose and bacteria cellulose), fibroin, and its any combination.
Biodegradable material is alternatively synthesized degradable material, for example, aliphatic polyester, poly- hydroxyl valerate (PHV),
Poly butyric ester (PHB), poly butylene succinate (PBS)), polyglycolic acid (PGA), polylactic-co-glycolic acid
(PLGA), poe (POE), degradability polyurethane (such as starch conversion polyurethane), polyvinyl alcohol, poly- to dioxocyclohex
Ketone, poly-p-dioxanone, poly- dioxane ketone, polytetramethylene carbonate diol, polyphosphazene and its any combinations.
Alternatively, outer wall 1 is biological non-degradable material, such as nylon, terylene, polypropylene, polyethylene, polytetrafluoroethyl-ne
Alkene, silicon rubber, fluorosioloxane rubber, natural rubber, polyacrylate, aromatic polyester (such as polyethylene terephthalate
(PET)), nondegradation polyurethane, polyether-ether-ketone, polyacrylonitrile, polysiloxanes, polyformaldehyde, polyvinyl chloride and its any group
Close.
Alternatively, outer wall 1 is natural material, such as the lumen organization of animal.
In the present embodiment, outer wall 1 and biological construct 2 are in a tubular form；Biological construct 2 is set in outer wall 1, and is attached
On the inner surface of outer wall 1.
Can also preferably, biological construct 2 in the form of sheets, strip, bulk or tubulose, have defective biology for repairing
Lumen organization.
Preferably, bioactive substance includes micro-capsule, and micro-capsule includes the cell of animal and is wrapped in extracellular biofacies
Capacitive material.Multiple micro-capsules arrange to form the biological construct of sheet, strip, bulk or tubulose by ranks.By active material system
It is standby into biological construct 2 so that the biology performance of biological construct 2 is adapted with host, avoids host and biological construct 2
There is the phenomenon to reject.In the present embodiment, biological construct 2 is combined with the inner surface of outer wall 1 by bonding.
Alternatively, lumen organization's construct is alimentary canal lumen organization construct, respiratory tract lumen organization construct, lymph
Pipe lumen organization construct or vessel lumen tissue construct.
Exemplary embodiment of the present utility model is the foregoing is only, it is all in this reality not to limit the utility model
Within new spirit and principle, any modification, equivalent substitution and improvements made etc., it should be included in of the present utility model
Within protection domain.
Exemplary embodiment of the present utility model is these are only, it is all new in this practicality not to limit the utility model
Within the spirit and principle of type, any modification, equivalent substitution and improvements made etc., protection of the present utility model should be included in
Within the scope of.
Claims (12)
- A kind of 1. preparation facilities of lumen organization's construct, it is characterised in that including：First supporting part, for carrying the outer wall (1) of lumen organization's construct；Second supporting part (4), for carrying the biological construct (2) of lumen organization's construct；AndDrive mechanism, for making first supporting part and second supporting part (4) to relatively move, so as to the biology Construct (2) is attached on the inner peripheral surface of the outer wall (1).
- 2. preparation facilities according to claim 1, it is characterised in that also include being used to be tensioned or expand the outer wall (1) Pipe expander.
- 3. preparation facilities according to claim 2, it is characterised in that the pipe expander includes cavity (5) and is arranged on Air entry (11) on the cavity (5), first supporting part include tube-like piece (3), and the tube-like piece (3) is set in described The outside of outer wall (1), aperture (9) is provided with the tube-like piece (3), the tube-like piece (3) is arranged in the cavity (5).
- 4. preparation facilities according to claim 3, it is characterised in that the tube-like piece (3) and the inner circumferential of the cavity (5) There is gap between face, the gap along the tube-like piece (3) circumferentially.
- 5. preparation facilities according to claim 3, it is characterised in that the cavity (5) has opening end, the tube-like piece (3) inserted by the opening end in the cavity (5), one end of the remote opening end of the tube-like piece (3) is closing 's.
- 6. preparation facilities according to claim 5, it is characterised in that the cavity (5) has relative with the opening end Bottom, the bottom sets fluted, and one end of the remote opening end of the tube-like piece (3) is plugged in the groove In.
- 7. preparation facilities according to claim 5, it is characterised in that the close opening end of the tube-like piece (3) One end is provided with boss (7), and the boss (7) is prolonged by the periphery of the tube-like piece (3) towards the inner peripheral surface of the cavity (5) Stretch, the boss (7) along the tube-like piece (3) circumferentially, between the inner peripheral surface of the boss (7) and the cavity (5) It is provided with seal (8).
- 8. preparation facilities according to claim 7, it is characterised in that the end of the direction cavity (5) of the boss (7) Holding tank is provided with face, the seal (8) is arranged in the holding tank.
- 9. preparation facilities according to claim 2, it is characterised in that the pipe expander is also included along the outer wall (1) Outer peripheral face extension fluid passage.
- 10. preparation facilities according to claim 9, it is characterised in that axial direction of the fluid passage along the outer wall (1) Extension, or circumference of the fluid passage along the outer wall (1) extend spirally.
- 11. preparation facilities according to claim 1, it is characterised in that also include being used for for the outer wall (1) and/or life The spray gun of thing construct (2) spraying adhesive.
- 12. preparation facilities according to claim 11, it is characterised in that the spray gun can be relative with the outer wall (1) Movement, the spout of the spray gun can be towards the inner surface of the outer wall (1)；OrThe spray gun and the biological construct (2) can be relative movement, the spout of the spray gun can be towards the biology The outer surface of construct (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621053494.0U CN206621452U (en) | 2016-09-14 | 2016-09-14 | The preparation facilities of lumen organization's construct |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621053494.0U CN206621452U (en) | 2016-09-14 | 2016-09-14 | The preparation facilities of lumen organization's construct |
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| Publication Number | Publication Date |
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| CN206621452U true CN206621452U (en) | 2017-11-10 |
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| Country | Link |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107411845A (en) * | 2016-09-14 | 2017-12-01 | 四川蓝光英诺生物科技股份有限公司 | Lumen organization's construct, lumen organization's structure preparation and its device |
| US20210268716A1 (en) * | 2018-06-19 | 2021-09-02 | Revotek Co., Ltd | Manufacturing method of lumen tissue construct |
| CN113696500A (en) * | 2021-10-29 | 2021-11-26 | 极限人工智能(北京)有限公司 | Device and method for assembling adjustable vertebral joint assembly and flexible tube |
-
2016
- 2016-09-14 CN CN201621053494.0U patent/CN206621452U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107411845A (en) * | 2016-09-14 | 2017-12-01 | 四川蓝光英诺生物科技股份有限公司 | Lumen organization's construct, lumen organization's structure preparation and its device |
| US20210268716A1 (en) * | 2018-06-19 | 2021-09-02 | Revotek Co., Ltd | Manufacturing method of lumen tissue construct |
| CN113696500A (en) * | 2021-10-29 | 2021-11-26 | 极限人工智能(北京)有限公司 | Device and method for assembling adjustable vertebral joint assembly and flexible tube |
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