CN2066028U - Automatic gene augmentor - Google Patents
Automatic gene augmentor Download PDFInfo
- Publication number
- CN2066028U CN2066028U CN 89220720 CN89220720U CN2066028U CN 2066028 U CN2066028 U CN 2066028U CN 89220720 CN89220720 CN 89220720 CN 89220720 U CN89220720 U CN 89220720U CN 2066028 U CN2066028 U CN 2066028U
- Authority
- CN
- China
- Prior art keywords
- bath
- water
- temperature
- control
- thermostatic control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an automatic device conducting DNA polymerase chain reaction and implementing gene amplification. The automatic device is characterized in that three aqueous thermostats compose integration and replace manual operation via micro computer controlling manipulators, automatically completing polymerase chain third step heat cycle heat reaction and implementing gene amplification automation. Therefore, the utility model can solve the problems of high work intensity, excessive human power consumption, easy error, etc. in the manual operation of the prior art. The utility model belongs to an instrumental innovation applied to biological medicine and genetic engineering.
Description
The utility model belongs to the innovation of the instrument of biomedicine and genetic engineering.
The prior art of carrying out gene amplification is: adopt temperature to be respectively three waters bath with thermostatic control (1,2,3) of 92 ℃, 42 ℃ and 72 ℃, method of operating by hand, to the sample disc (11) of sample be housed, in each water-bath, soak certain hour, change another water-bath again over to, so circulate 20-60 time.
The purpose of this utility model is a kind of automatic gear of finishing DNA gene amplification reliably of design, provides a kind of convenience self-reacting device accurately for carrying out archaeal dna polymerase chain reaction (PCR).
Contour structures of the present utility model such as Fig. 1.Instrument comprises water bath with thermostatic control (1,2,3), sample disc (11) and mechanical manipulator (4) three parts.Realize the technical solution of the utility model such as Fig. 2.Three water-baths are combined in the cabinet, and respectively as sample sex change water bath with thermostatic control (1), annealing water bath with thermostatic control (2) and primer prolong water bath with thermostatic control (3).The temperature of water-bath is by corresponding radiator valve (7) difference closed loop temperature control.Each water-bath is equipped with agitator (5) and stirring rod (6).The temperature of each water-bath is shown by temperature digital display (8).Mechanical manipulator (4) is made up of vertical transmission rig (9) and horizontal transmission rig (10).Its action is subjected to minicomputer (14) control and passes through vertical control interface circuit (12) and horizontal control interface circuit (13), make topworks be with sample disc (11) to make vertical displacement movement and horizontally rotate motion, thereby make sample disc finish the stop of the certain hour in each water-bath and the circulation of certain number of times according to the different parameters that sets, reach every requirement of polymerase chain reaction (PCR), realize DNA cloning.
Main points of the present utility model are, adopt the mode of mechanical manipulator, realize sample disc rising and falling automatically and moving in each water-bath.The cycle index that time that the temperature of each water-bath, sample disc stop in water-bath and sex change, annealing, primer prolong can be set up on their own by the user as required.
The utility model is combined into one three waters bath with thermostatic control and replaces manual operation by microcomputerized control machinery hand, realized DNA gene amplification automatization, thus solved the labour intensity that the prior art manual operations brings big, consume many, the problem such as make mistakes easily of manpower.
Embodiment of the present utility model is as follows:
1. with sex change water bath with thermostatic control (1), annealing water bath with thermostatic control (2) and primer prolong water bath with thermostatic control (3) and are combined in the cabinet.Water-bath is adopted 400 watts, and 220 volts exchange heating collar (27) heating and temperature automatically controlled by corresponding radiator valve (7), circuit such as Fig. 5 of radiator valve (7).D
1For PN junction type temperature sensor, this transmitter is placed water-bath, as temperature detection and feedback element.W1 is the preset temperature potentiometer, and by regulating this potentiometer, bath temperature can be set arbitrarily 30 ℃～100 ℃ scopes.The PID that is made up of 741 type operational amplifiers and ZK-50 type scr voltage converter regulates, and silicon controlled rectifier closed loop temperature-adjusting circuit is realized the water bath with thermostatic control temperature auto control by the voltage that changes input heating collar (27).The real time temperature of water-bath is shown by JKT-5612 type three bit digital panel tables (8) behind the waver gating.
2. the stirring of water-bath produces rotatingfield by magnetic stirrer (5) and drives the stirring rod (6) that the soft iron core in the water-bath makes and carry out.Fig. 3 is the concrete structure figure of magnetic stirrer (5).It is made up of magnet (15), magnet frame (16), electric machine support (17), ND-D reversible electric machine (18) and pillar (19).
3. concrete structure such as Fig. 4 of mechanical manipulator (4), wherein horizontal rotating arm (20), turning axle (21) and stepped start-stop system 55BF type horizontally rotate drive-motor (22) and have constituted and horizontally rotate transmission rig (10); Lifting shaft (23), slide block (24), fork (25) and reciprocating type 20 type VTOL (vertical take off and landing) drive-motor (26) have been formed vertical transmission rig (9).Fig. 4 gives the mutual relationship of mechanical manipulator (4) and sample disc (11) and water-bath (1,2,3) in addition.
4. Fig. 6 is the electrical schematic diagram of vertical control interface circuit of minicomputer (12) and horizontal control interface circuit (13).Give the PIO interface sheet of TP801 minicomputer (14) and concrete annexation between interface circuit (12) and (13) and the concrete annexation between interface circuit (12), (13) and execution unit (22) and (26) among the figure.
Claims (4)
1, a kind of novel gene-amplificative instrament, by three waters bath with thermostatic control [1,2,3] and sample disc [11] form, it is characterized in that sex change water bath with thermostatic control [1], annealing water bath with thermostatic control [2], primer extension water bath with thermostatic control [3] is combined into one, three water-baths drive by magnetic stirrer [5] that stirring rod [6] stirs so that in the water-bath temperature even, temperature is by radiator valve [7] control automatically, temperature value shows by temperature digital display [8], the sample mouth dish [11] that is inserted with sample hose is made vertical displacement movement and is horizontally rotated motion under the drive of mechanical manipulator [4], the exercises of mechanical manipulator are by minicomputer [14] control automatically.
2, according to the amplification of the automatic gene in the claim 1 instrument, it is characterized in that three waters bath with thermostatic control (1,2,3) are combined in the cabinet, the temperature of each water-bath can be set arbitrarily 30 ℃～100 ℃ scopes as required, each water-bath and the magnetic stirrer (5) that is provided with are made up of motor (18) and magnet (15), and stirring rod (6) is made by soft iron core.
3, according to the amplification of the automatic gene in the claim 1 instrument, it is characterized in that radiator valve (7) adopts PIO to regulate, silicon controlled rectifier closed loop temperature control, temperature is shown by digital indicator (8).
4, according to the amplification of the automatic gene in the claim 1 instrument, it is characterized in that mechanical manipulator (4) is made up of vertical transmission rig (9) and horizontal transmission rig (10), minicomputer (14) drives sample disc (11) by interface circuit (12) and (13) control mechanical manipulators (4) and horizontally rotates and vertical displacement movement, and its execution unit is motor (22) and (26).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89220720 CN2066028U (en) | 1989-12-12 | 1989-12-12 | Automatic gene augmentor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 89220720 CN2066028U (en) | 1989-12-12 | 1989-12-12 | Automatic gene augmentor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2066028U true CN2066028U (en) | 1990-11-21 |
Family
ID=4875342
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 89220720 Withdrawn CN2066028U (en) | 1989-12-12 | 1989-12-12 | Automatic gene augmentor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2066028U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102176125A (en) * | 2011-02-22 | 2011-09-07 | 武汉馨世生物科技有限公司 | General intelligent control device for polymerase chain reaction |
| CN102242051A (en) * | 2010-05-13 | 2011-11-16 | 私立中原大学 | Polymerase chain reaction device |
| CN106399087A (en) * | 2016-10-27 | 2017-02-15 | 杭州安杰思生物科技有限公司 | Single-pore temperature switching device of molecular diagnosis system |
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
-
1989
- 1989-12-12 CN CN 89220720 patent/CN2066028U/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242051A (en) * | 2010-05-13 | 2011-11-16 | 私立中原大学 | Polymerase chain reaction device |
| CN102176125A (en) * | 2011-02-22 | 2011-09-07 | 武汉馨世生物科技有限公司 | General intelligent control device for polymerase chain reaction |
| CN106399087A (en) * | 2016-10-27 | 2017-02-15 | 杭州安杰思生物科技有限公司 | Single-pore temperature switching device of molecular diagnosis system |
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103897987A (en) | Automatic extraction device and method of nucleic acid based on nano magnetic beads | |
| CN2066028U (en) | Automatic gene augmentor | |
| CN204097451U (en) | Multifunctional fermentation tank | |
| US20210162356A1 (en) | Device and method for mixing and bubble removal | |
| CN107930716B (en) | Automatic rotary gradient constant-temperature water bath kettle for detection chamber and use method thereof | |
| CN207210391U (en) | A kind of lifting structure and temperature metal bath | |
| CN102160760A (en) | Stirrer | |
| CN217173749U (en) | Self-detection constant-temperature closed enzymatic hydrolysis device with temperature regulation function | |
| CN2820312Y (en) | Integrated oil bath electromagnetic stirring heater | |
| CN215877906U (en) | Constant-temperature production reaction device for leather auxiliaries | |
| RU71118U1 (en) | UNIVERSAL AUTOMATED INSTALLATION FOR CULTIVATION OF PHOTO-DEPENDENT MICROORGANISMS | |
| CN215139192U (en) | Agitated vessel and battery production line | |
| CN107354085A (en) | A kind of lifting structure, temperature metal bath and the method for realizing temperature difference | |
| CN208878332U (en) | It is a kind of environmental protection tubing production use raw material blending device | |
| CN2738978Y (en) | Protective-cover | |
| CN207805652U (en) | Sensing chamber's automatic rotary gradient constant-temperatureactivation water-bath | |
| CN208245076U (en) | A kind of no brush stator automatic paint dripping machine | |
| CN202212138U (en) | Constant-temperature magnetic stirring device | |
| CN214654969U (en) | Biological assay is with drawing operation panel that can carry out control by temperature change | |
| CN206103856U (en) | Modified asphalt high speed shearing machine | |
| CN2436222Y (en) | Premixing tank for liquid mixing and agitating | |
| CN218659826U (en) | Experimental cement paste mixer | |
| CN212770745U (en) | Full-automatic gene amplification instrument | |
| CN209438523U (en) | Temperature control magnetic stirring apparatus | |
| CN215856123U (en) | Electric heating constant temperature incubator capable of accurately controlling temperature |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Designer after: Zhu Yichuan Designer after: Liu Yaoqing Designer after: Zhang Jiaxuan Designer after: Zhao Shuqian Designer after: Xu Shikun Designer after: Zhang Jing Designer after: Gu Shengli Designer after: Gao Hong Designer after: Qi Li Designer after: Ma Liren Designer before: Zhu Yichuan Designer before: Zhang Jiaxuan Designer before: Zhao Shuqian Designer before: Xu Shikun Designer before: Zhang Jing Designer before: Gu Shengli |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: DESIGNER; FROM: ZHU YICHUAN; ZHANG JIAXUN; ZHAO SHUQIAN; XU SHIKUN; ZHANG JING; GU SHENGLITO: ZHU YICHUAN; ZHANG JIAXUN; ZHAO SHUQIAN; XU SHIKUN; ZHANG JING; GU SHENGLI; GAO JIANG; QI LI; MA LIREN; LIU YAOQING |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C15 | Extension of patent right duration from 15 to 20 years for appl. with date before 31.12.1992 and still valid on 11.12.2001 (patent law change 1993) | ||
| RN01 | Renewal of patent term | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |